Materials Science & Engineering C-Materials for Biological Applications最新文献

{"title":"Near-infrared-responsive Ag/CuO@PDA nanowire platform for photothermal-amplified chemodynamic/ion-synergistic antibacterial therapy and infected wound healing.","authors":"Silu Sun, Yujiang Liu, Yuhui Shi, Yiting Liu, Hailiang Pei, Di Huang, Zhiyuan Feng","doi":"10.1016/j.bioadv.2026.215134","DOIUrl":"https://doi.org/10.1016/j.bioadv.2026.215134","url":null,"abstract":"<p><p>Biofilm-associated wound infections and antimicrobial resistance urgently require non-antibiotic therapies that integrate efficient antibacterial activity with tissue repair. Herein, we developed a sandwich-like trilayer Ag/CuO@PDA nanoplatform composed of one-dimensional CuO nanowires, Ag nanoparticles, and a polydopamine (PDA) photothermal shell. Under 980 nm near-infrared (NIR) irradiation, PDA-mediated local heating enhanced CuO-driven reactive oxygen species (ROS) generation and significantly promoted Ag⁺/Cu²⁺ release, as verified by ICP-MS analysis. Ag/CuO@PDA combined with NIR showed potent broad-spectrum antibacterial activity against Methicillin-resistant Staphylococcus aureus (MRSA)and Escherichia coli (E. coli) and effectively disrupted mature biofilms by damaging bacterial membranes, increasing membrane permeability, and inducing intracellular component leakage. Meanwhile, the nanoplatform exhibited favorable cytocompatibility and supported wound-healing-related cellular behaviors, including cell migration and endothelial tube formation. In an Staphylococcus aureus (S. aureus) -infected wound model, Ag/CuO@PDA + NIR markedly reduced bacterial burden, alleviated inflammation, promoted collagen deposition and angiogenesis, and accelerated wound closure without obvious systemic toxicity. Overall, this trilayer nanoplatform integrates photothermal conversion, ROS generation, and metal ion-mediated antibacterial action, offering a promising non-antibiotic strategy for treating biofilm-associated infected wounds while promoting tissue repair.</p>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"190 ","pages":"215134"},"PeriodicalIF":6.0,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148889205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Database-guided identification of high-performance cell line for robust human cell-derived ECM hydrogel fabrication.","authors":"Hongju Xu, Chen Li, Linqiang Wu, Jiaqi Yang, Junyi Ji, Shaoyu Liu, Yaowen Wang, Hao Zheng, Yuyan Zhu, Yijun Zheng, Jiesi Luo","doi":"10.1016/j.bioadv.2026.215138","DOIUrl":"https://doi.org/10.1016/j.bioadv.2026.215138","url":null,"abstract":"<p><p>Extracellular matrix (ECM) hydrogels are essential for recapitulating native microenvironments in fundamental biomedical research, yet conventional animal tissue-derived products face challenges of cross-species variability or donor-related inconsistency. Human cell-derived matrix (hCDM) fabricated via cell sheet technology offers a promising alternative; however, its efficient fabrication remains constrained by the limited expansion capacity and inconsistent ECM deposition behavior of commonly used primary cells. To facilitate rational cell-source selection, we established a database-guided screening strategy integrating extracellular matrix-related expression profiles, proliferative characteristics, and commercial accessibility. Using primary human dermal fibroblasts (HDFs) as a functional reference, Hs 578 T cells emerged as a top-ranked candidate exhibiting strong ECM deposition potential together with robust proliferative capacity. In vitro validation confirmed that Hs 578 T cells exhibited ECM deposition capacity significantly exceeding that of HDFs. Under optimized serum-reduced conditions, Hs 578 T cells formed cohesive, protein-rich cell sheets that were successfully processed into structurally stable hCDM hydrogels retaining abundant collagens and other critical ECM components. Functional assessment demonstrated that the resulting hCDM hydrogel supports endothelial cell culture and three-dimensional vascular network formation at levels comparable to collagen type I hydrogel. These findings establish a database-guided workflow for rational seed cell selection, providing a strategy that bridges cell sheet cultivation with the efficient fabrication of human ECM biomaterials. STATEMENT OF SIGNIFICANCE: Developing human ECM biomaterials via cell sheet technology is frequently constrained by the inherent variability and limited expansion of primary seed cells. This study introduces a database-guided screening strategy integrating transcriptomic profiles, growth kinetics, and commercial availability to systematically identify high-performance human cell lines for matrix fabrication. By targeting cells with superior biosynthetic and proliferative traits, we demonstrate that a representative cell line, Hs 578 T, can produce ECM-rich substrates with enhanced efficiency and consistency compared to conventional fibroblasts. Under optimized conditions, Hs 578 T cells formed protein-rich cell sheets processed into ECM hydrogels retaining native complexity and pro-vascular bioactivity. This workflow enables rational seed cell prioritization, bridging cell sheet technology with the efficient fabrication of bioactive human ECM materials.</p>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"190 ","pages":"215138"},"PeriodicalIF":6.0,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148867794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunosuppressive microparticles for allogeneic transplantation in nerve repair.","authors":"Holly N Harrison, Matthew Whitty, Gedion Girmahun, Omar Matar, Eesha Mehrotra, Gareth R Williams, James B Phillips, Victoria H Roberton","doi":"10.1016/j.bioadv.2026.215129","DOIUrl":"https://doi.org/10.1016/j.bioadv.2026.215129","url":null,"abstract":"<p><p>Peripheral nerve damage affects millions of people each year, possibly resulting in long-term functional deficits and chronic pain. Severe nerve injury is largely treated with autologous grafting, which produces donor site morbidity and has limited supply. Although cell therapies and living allografts are promising alternatives, their clinical translation is hampered in part due to their immunogenicity. Local delivery of immunosuppressants via controlled-release microparticles could protect both allografts and cellular constructs, while potentially avoiding the detrimental effects of systemically administered immunosuppressants. To produce immunosuppressant-loaded engineered nerve grafts, tacrolimus-loaded microparticles were encapsulated into Schwann cell-laden Engineered Neural Tissue (EngNT) constructs. We investigated cell viability and alignment, then used these constructs to repair 10 mm nerve defects in major histocompatibility complex (MHC) mismatched rats. Schwann cell viability and alignment in EngNT remained robust in the presence of the microparticles, although CD4⁺ and CD8⁺ T cell infiltration into the grafts was not significantly reduced by the tacrolimus-loaded material. To produce immunosuppressant-coated nerve allografts, tacrolimus microparticles were incorporated into alginate to form a coating. We studied the mechanical behaviour of native and coated nerve grafts, then implanted these grafts in 25 mm nerve defects in MHC-I and II mismatched rats. The coating did not significantly alter tensile nerve stiffness, and we observed a significant reduction in CD8⁺ T cell infiltration into the allografts with a tacrolimus-loaded microparticle coating. These findings demonstrate the feasibility of preparing allogeneic nerve grafts pre-loaded with local immunosuppressant and the potential for these biomaterials to locally modulate the immune response, enhancing the translational potential for allogeneic grafting in nerve repair.</p>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"190 ","pages":"215129"},"PeriodicalIF":6.0,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148867779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and functional validation of groove structures differentially regulating endothelial/smooth muscle cell behavior.","authors":"Juan Yan, Songhao Liu, Caixia Li, Yang Si, Liang Gao, Hongxia Yang, Mengyu Gao","doi":"10.1016/j.bioadv.2026.215104","DOIUrl":"https://doi.org/10.1016/j.bioadv.2026.215104","url":null,"abstract":"<p><p>Drug-eluting stents inhibit smooth muscle cell proliferation but simultaneously impede endothelial repair, leaving restenosis risk still prominent. Surface microstructures, as a physical modulation approach, hold promise for improving cell selectivity; however, a systematic study on how to select the optimal topography from multiple configurations to precisely balance the behaviors of two cell types is lacking. In this work, we designed six microstructures and performed a systematic screening. We found that groove structure V (with a groove width of ~3.73 μm, depth of ~1.00 μm, and ridge width of ~2.33 μm) not only significantly promoted the proliferation of human coronary artery endothelial cells (HCAECs) but also maximally inhibited the excessive proliferation of human coronary artery smooth muscle cells (HCASMCs), thereby achieving the greatest differentiation in proliferative responses between the two cell types and enabling a differential regulation favoring endothelialization. After transferring this optimal structure onto nickel‑titanium alloy surfaces, we further verified that it enhanced the adhesion, migration, and competitive growth of human umbilical vein endothelial cells (HUVECs) and induced necrosis of smooth muscle cells (HUVSMCs), significantly accelerating the re-endothelialization process. By combining microstructure screening with nickel‑titanium alloy application, this study demonstrates that metallic surface topography can actively modulate differential cellular responses, providing a new basis for the biocompatible design of vascular stents.</p>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"190 ","pages":"215104"},"PeriodicalIF":6.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148882475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced strategies for biofunctionalizing cardiovascular implants using cell-derived materials.","authors":"Md Lemon Hasan, Mohammad Faysal Al Mazid, Yeasin Khan, Shihab Uddin, Md Anwarul Hasan, Khandoker Asiqur Rahaman","doi":"10.1016/j.bioadv.2026.215131","DOIUrl":"https://doi.org/10.1016/j.bioadv.2026.215131","url":null,"abstract":"<p><p>Functionalization of cardiovascular implants is advancing to improve hemocompatibility, endothelialization, anti-inflammatory effects, and long-term patency. Conventional materials, including polymers, chemical grafts, and bioactive molecules, provide important biological benefits but do not fully replicate the complex biochemical and physiological cues of the native vascular microenvironment. Cell-derived materials, including decellularized extracellular matrix (dECM), cell membranes, extracellular vesicles (EVs), and secretomes, offer significant opportunities by preserving native structural proteins, adhesion molecules, lipid interfaces, and paracrine signaling factors. These biologically derived materials enhance endothelial repair, modulate inflammation, reduce neointimal hyperplasia, and promote vascular remodeling. Despite these advantages, substantial translational challenges remain, as many biofunctionalized implants exhibit inadequate coating adhesion, limited shear resistance, poor durability under pulsatile flow, and susceptibility to fatigue, delamination, or structural failure. This review summarizes recent advances in the functionalization and fabrication of cardiovascular implants using cell-derived materials, providing a systematic analysis of fabrication strategies, biological performance, mechanical properties, and device-specific challenges. Finally, we discuss common failure mechanisms, translational barriers, and future directions for developing scalable, mechanically robust, and clinically translatable cell-derived biomaterials for next-generation cardiovascular implants.</p>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"190 ","pages":"215131"},"PeriodicalIF":6.0,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148876665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-functional β-TCP based injectable bone grafts functionalized with peptides for enhanced osteogenesis and broad-spectrum biofilm inhibition","authors":"Eda Bilgiç ,&nbsp;Şevval Özkaya ,&nbsp;Duygu Gençer ,&nbsp;Ozan Karaman ,&nbsp;Günnur Pulat","doi":"10.1016/j.bioadv.2026.214739","DOIUrl":"10.1016/j.bioadv.2026.214739","url":null,"abstract":"<div><div>Bone defects with irregular geometries and high infection risk remain a major clinical challenge. Injectable bone grafts (IBGs) offer minimally invasive and moldable solutions, yet conventional β-tricalcium phosphate (β-TCP)-based formulations often lack sufficient mechanical strength and antimicrobial activity. Here, a dual-functional β-TCP-based putty-form IBG was developed by combining powdered and sintered granules at optimized ratios to enhance mechanical stability, osteogenic potential, and handling properties. Antimicrobial peptides (AMPs), KR-12 and its anti-MRSA analog KR-12-a5, were covalently immobilized onto β-TCP surfaces via cold atmospheric plasma (CAP), which created reactive sites without compromising structural integrity to ensure stable peptide conjugation and sustained antimicrobial activity. The AMP-functionalized IBGs demonstrated potent anti-biofilm activity against <em>Staphylococcus aureus</em>, <em>Escherichia coli</em>, multidrug-resistant <em>Pseudomonas aeruginosa</em>, and MRSA with KR-12-a5, while KR-12 more effectively promoted human mesenchymal stem cell (hMSC) viability, osteogenic differentiation, and extracellular matrix deposition. Osteogenic markers were analyzed using alkaline phosphatase (ALP) activity and collagen deposition to assess protein levels, and the expression of OCN, OPN, COL1, ALP and RUNX2 genes was evaluated by quantitative PCR (qPCR). To our knowledge, this is the first injectable bone graft that simultaneously integrates osteogenic and broad-spectrum anti-biofilm functionalities for treating complex, infection-prone, and irregularly shaped bone defects.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"183 ","pages":"Article 214739"},"PeriodicalIF":6.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146081884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoceria-mediated redox modulation for periodontal management: Mechanisms, applications, and challenges","authors":"Xin Chen ,&nbsp;Song Chen ,&nbsp;Yi Hou","doi":"10.1016/j.bioadv.2026.214752","DOIUrl":"10.1016/j.bioadv.2026.214752","url":null,"abstract":"<div><div>Periodontitis is caused by dental plaque that triggers the host immune responses by the dysregulation of reactive oxygen species (ROS), leading to the destruction of local tissues such as gingiva, periodontal ligament, and alveolar bone. With its high prevalence, periodontitis impacts the oral health of billions worldwide. Clinical therapy for periodontitis relies on mechanical debridement and adjunctive antibiotics, strategies that often result in incomplete efficacy and a high recurrence rate. The development of cerium oxide nanoparticles (nanoceria), which exhibits enzyme-like catalytic activity and biocompatibility, enables targeted redox modulation to restore ROS balance, showing promise for clinical treatment. Based on above, this article focuses on the pathogenesis of periodontitis and the regulatory functions of ROS, and summarizes the design principles, functional engineering, and therapeutic mechanisms of nanoceria for periodontal therapy. Furthermore, the review outlines preventive strategies against periodontitis based on nanoceria. It then discusses the associated clinical challenges and future prospects. Overall, this work provides a comprehensive overview of nanoceria as the redox-based strategy for periodontal management.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"183 ","pages":"Article 214752"},"PeriodicalIF":6.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146138120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-mediated blood-brain barrier opening for targeted neurological drug delivery","authors":"Zibo Qin ,&nbsp;Zhangbaihe Wang ,&nbsp;Cancan Gao ,&nbsp;Xueqing Yong ,&nbsp;Yue Hua ,&nbsp;Ying Zhou ,&nbsp;Jinbing Xie","doi":"10.1016/j.bioadv.2026.214754","DOIUrl":"10.1016/j.bioadv.2026.214754","url":null,"abstract":"<div><div>Neurological disorders represent a devastating global health crisis, and the blood-brain barrier (BBB) remains a major obstacle for their treatment. Conventional strategies for BBB opening, including direct intracranial injection, osmotic disruption, receptor-mediated transcytosis, and nanoparticle carriers, often suffers from surgical invasiveness, systemic toxicity, poor biodistribution, and off-target effects. Ultrasound-mediated drug delivery has emerged as a revolutionary non-invasive technology for transient and targeted BBB opening, enabling enhanced penetration of therapeutic agents into the central nervous system. This review comprehensively summarizes the mechanisms underlying ultrasound-based delivery with focus on current delivery platforms including microbubble (MB)-assisted, nanoparticle-based, and MB-nanoparticle composite strategies. Furthermore, we highlight recent advances in the application of focused ultrasound (FUS) combined with MBs for the treatment of Alzheimer's disease, Parkinson's disease, and glioma. Finally, we discuss emerging technologies such as sonodynamic therapy and ultrasound-controlled magnetic nanorobots, while also addressing current challenges in this field. This review underscores the transformative potential of ultrasound-mediated drug delivery as a versatile platform for precision neurology. It also prospects future directions for advancing multidisciplinary research and clinical translation.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"183 ","pages":"Article 214754"},"PeriodicalIF":6.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146159058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomodulatory and pro-mineralizing effects of an injectable baicalein-loaded methacrylated gelatin hydrogel for vital pulp therapy","authors":"Beatriz Ometto Sahadi ,&nbsp;Igor Paulino Mendes Soares ,&nbsp;Chloe Gifford ,&nbsp;Caroline Anselmi ,&nbsp;Pedro Henrique Chaves de Oliveira ,&nbsp;Renan Dal-Fabbro ,&nbsp;Maedeh Rahimnejad ,&nbsp;Marcelo Giannini ,&nbsp;Marco C. Bottino","doi":"10.1016/j.bioadv.2026.214768","DOIUrl":"10.1016/j.bioadv.2026.214768","url":null,"abstract":"<div><div>This study first investigated the biological function of baicalein (BA) and then developed a photocrosslinkable methacrylated gelatin (GelMA) hydrogel incorporating BA-loaded, carboxylated mesoporous silica nanospheres (MSNs-COOH-BA) for vital pulp therapy. Initially, BA (1–20 μM) was tested for cytocompatibility, <em>in vitro</em> mineralized nodule formation as an early indicator of odontogenic potential, and antioxidant/anti-inflammatory functionality on dental pulp stem cells (DPSCs) and macrophages. Then, 15% (w/v) GelMA was formulated with MSNs-COOH-BA (10 or 20 mg/mL). Hydrogels were characterized by SEM/EDS for their microstructure morphology and chemical composition, as well as for compression, swelling, degradation, and BA release. Biological assessments included DPSC cytocompatibility and early mineralization responses under or without LPS stimulation, macrophage cytokine modulation, and <em>in vivo</em> subcutaneous biocompatibility in rats. Statistical analysis used ANOVA/post-hoc tests (α = 5%). BA was non-cytotoxic (≥70% viability at 24 h), enhanced mineralized nodule formation under both basal and inflammatory conditions, reduced intracellular ROS levels, and suppressed TNF-α, IL-1α, and IL-6 production in a dose-responsive manner. GelMA maintained its porous architecture after MSN incorporation. Although BA-functionalized MSNs showed some nanosphere clustering, they reinforced mechanical performance, with MSNs-COOH-BA (20 mg/mL) increasing Young's modulus and ultimate compressive strength relative to GelMA and outperforming MSNs without BA. MSNs-containing hydrogels displayed moderated swelling and slower enzymatic mass loss <em>versus</em> GelMA alone. BA was released over 10 days, and eluates remained non-cytotoxic (&lt;30% reduction <em>vs</em> control). Under LPS challenge, 20 mg/mL MSNs-COOH-BA induced the highest 21-day mineralized nodule formation in DPSCs, and hydrogel extracts reduced macrophage synthesis of TNF-α and IL-1α. <em>In vivo</em>, all groups exhibited an acute infiltrate at 7 days, which significantly declined by 28 days, with no differences observed among formulations at either time point. The GelMA/MSNs-COOH-BA hydrogel paired sustained BA delivery with mechanical integrity, cytocompatibility, anti-inflammatory activity, and early pro-odontogenic cellular responses, supporting its promise as an injectable biomaterial with clinically relevant therapeutic properties for preserving pulp vitality while supporting the dentin-pulp complex's intrinsic repair and development abilities.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"183 ","pages":"Article 214768"},"PeriodicalIF":6.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146191617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coaxial bioprinting of microsphere bioink to engineer heterogeneous vascularized lung cancer model","authors":"Qiulei Gao ,&nbsp;Zhongwei Guo ,&nbsp;Shiqiang Zhang ,&nbsp;Jingjing Xia ,&nbsp;Junfu Li ,&nbsp;Tianying Yuan ,&nbsp;Jiyu Chen ,&nbsp;Yongcong Fang ,&nbsp;Jingjiang Qiu ,&nbsp;Ronghan Wei","doi":"10.1016/j.bioadv.2026.214741","DOIUrl":"10.1016/j.bioadv.2026.214741","url":null,"abstract":"<div><div>3D bioprinting is a promising strategy for engineering in vitro tumor models. However, replicating the intratumoral parenchyma-stroma heterogeneity remains challenging due to the poor formability of biomimetic bioinks. In this study, we developed a method to enable the direct extrusion of low-concentration gelatin-methacrylate (GelMA)/Matrigel by overcoming its rheological limitations. The bioink was then incorporated within a coaxial bioprinting system to engineer a defined tumor parenchyma-stroma interface. The coaxial lung cancer model featured a dual-layer tubular structure. In this structure, the inner microsphere bioink was designed to mimic the tumor parenchyma, and the surrounding HAMA/Fibrin hydrogel was used to reproduce the stroma. The model not only established the spatial heterogeneity but also recapitulated biological function such as fibroblast-driven angiogenesis, as demonstrated by a 3.4-fold increase in microvascular density and a 2.3-fold extension in total vessel length. Furthermore, the model exhibited 50-fold increase in drug resistance compared to two-dimensional (2D) cultures. Additionally, the long-term cryopreservation stability and scalability endowed the model with the potential to be a tool for on-demand use. This work provides a potential platform for drug screening and mechanistic investigation of tumor biology.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"183 ","pages":"Article 214741"},"PeriodicalIF":6.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146081867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}