Materials Science & Engineering C-Materials for Biological Applications最新文献

{"title":"Functional hollow porous microfiber scaffold with BMP-2 for macrophages polarization and bone regeneration","authors":"Long Yang ,&nbsp;Yufei Liu ,&nbsp;Yong Yang ,&nbsp;Yuhu Zhou ,&nbsp;Zihao Zou ,&nbsp;Min He ,&nbsp;Changlei Yang ,&nbsp;Daizhu Yuan ,&nbsp;Jieheng Wu ,&nbsp;Chuan Ye","doi":"10.1016/j.bioadv.2025.214425","DOIUrl":"10.1016/j.bioadv.2025.214425","url":null,"abstract":"<div><div>Artificial bone substitutes have garnered significant attention within the field of biomaterials, and the use of tissue engineering technology to repair bone defects has emerged as a promising alternative. However, when artificial bone substitutes are implanted in the body, they can induce many adverse cellular and tissue immune responses. In this study, we use coaxial electrospinning technology to fabricate microfiber with controllable diameter, excellent mechanical properties and surface porous structure. Poly(3-hydroxybutyrate-<em>co</em>-4-hydroxybutyrate) (P34HB) and polyethylene glycol (PEG) were used as shell solutions and polyvinyl alcohol (PVA) and bone morphogenetic protein-2 (BMP-2) peptides as core solutions. Compared to PVA/P34HB-PEG (P/P-P) scaffolds, PVA-BMP-2/P34HB-PEG (P-B/P-P) scaffolds polarized more human monocytic leukemia cell line (THP-1 cells) toward alternatively activated macrophages (M2), as demonstrated by increased M2 and decreased classically activated macrophage (M1) phenotypic marker expression in the cells. P-B/P-P microfibers have the potential to regulate the polarization state of macrophages, induce them to differentiate into an M2 phenotype and inhibit the inflammatory response of surrounding tissues. In addition, microfibers with a hollow porous structure facilitate the controlled release of BMP-2 peptides by regulating the fiber diameter, enhancing the differentiation of pre-osteoblastic cell line derived from mouse calvaria (MC3T3-E1) cells in an osteogenic direction and facilitating the repair of bone defects in vivo. This study presents microfibers essential for modulating the osteoimmune microenvironment, facilitating efficient bone regeneration.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"177 ","pages":"Article 214425"},"PeriodicalIF":6.0,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144724627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Customized high-throughput microfluidic production of multifunctional porous microspheres for bone repair","authors":"Guangyu Hu ,&nbsp;Shuyun Zeng ,&nbsp;Tianao Shao ,&nbsp;Yong Li ,&nbsp;Yaoxian Han ,&nbsp;Jiawei Tu ,&nbsp;Jiahui Mao ,&nbsp;Yanbin Xiao ,&nbsp;Lei Zhang","doi":"10.1016/j.bioadv.2025.214426","DOIUrl":"10.1016/j.bioadv.2025.214426","url":null,"abstract":"<div><div>The development of bone repair materials has become a critical focus in addressing bone defects. Existing methods, such as autografts and allografts, are limited by issues like immune rejection and insufficient local anti-infection. Despite the promise of biocompatible scaffolds in drug and cell delivery, challenges persist in creating uniform, stable and injectable scaffolds with multifunctional properties for effective bone repair. Here, we present a customized coaxial microfluidic system designed for the high-throughput fabrication of PDTH porous microspheres (PDTH PMs). This system ensures precise control over microsphere size and structure, producing uniform, stable microspheres with interconnected pores that significantly enhance drug encapsulation efficiency. The PDTH PMs, composed of PLGA and functionalized with PDA and a hydrogel containing TOB and HA nanoparticles, demonstrate remarkable biocompatibility, ROS scavenging, and antibacterial properties. <em>In vitro</em>, these microspheres effectively promote BMSC proliferation, migration, and osteogenic differentiation. <em>In vivo</em>, using a rat calvarial bone defect model, PDTH PMs show significant improvement in bone regeneration, with increased bone mineral density and new bone formation. Biosafety <em>in vivo</em> assessments <em>via</em> histological examination and blood analysis confirm no adverse effects on major organs. Our work introduces a versatile method for bone defect repair, highlighting the potential of microfluidic-prepared multifunctional microspheres in bone regeneration strategies.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"177 ","pages":"Article 214426"},"PeriodicalIF":5.5,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144679139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boosted breast cancer treatment with cell membrane-coated PLGA nanocarriers: Investigating the interactions with various cell types","authors":"Laís Ribovski ,&nbsp;Paula Maria Pincela Lins ,&nbsp;Bruna J. Moreira ,&nbsp;Luana C. Antonio ,&nbsp;Juliana Cancino-Bernardi ,&nbsp;Valtencir Zucolotto","doi":"10.1016/j.bioadv.2025.214420","DOIUrl":"10.1016/j.bioadv.2025.214420","url":null,"abstract":"<div><div>Nanomaterials inspired by nature and applied in the medical field have exhibited remarkable potential to diagnose and treat diseases. However, further improvements are required to enhance therapeutic effectiveness, particularly in terms of targeting. In this study, we formulated paclitaxel (PTX)-loaded poly lactic-<em>co</em>-glycolic (PLGA) nanocarriers (PLGA-PTX NCs) and coated with cancer cell membrane derived from MCF-7 breast cancer cells, mPLGA-PTX NCs. By leveraging the homotypic adhesion between cells, we enhance the treatment's effectiveness, which is associated with increased accumulation of mPLGA NCs in MCF-7 cells. Additionally, the cellular uptake of mPLGA NCs is investigated in cell types similar to MCF-7, which may also promote homotypic adhesion through specific adhesion molecules. This study includes A549 lung cancer cells, HDFn dermal fibroblasts, and MCF-10A non-tumorigenic breast cells. Our results show higher uptake for all cell lines, indicating homologous binding and common cell adhesion molecules, regardless of specificity. The treatment's efficacy, as evidenced by cellular metabolic activity, implies that both the percentage of NC-positive cells and uptake levels should be considered when evaluating therapeutic potency. All findings together emphasize the importance of thorough analysis of nanocarrier-cell interactions covering various cell types and understanding the nature of these interactions.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"177 ","pages":"Article 214420"},"PeriodicalIF":5.5,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porous hydrogel of siloxane-modified polyvinyl alcohol/polydopamine containing berberine chloride, exhibiting robust antibacterial activity and promoting the healing of MRSA-infected wounds","authors":"Amirhossein Kiumarsi ,&nbsp;Mohammad Reza Farahpour ,&nbsp;Zohreh Ghazi Tabatabaei ,&nbsp;Hamed Hamishehkar","doi":"10.1016/j.bioadv.2025.214418","DOIUrl":"10.1016/j.bioadv.2025.214418","url":null,"abstract":"<div><div>Advanced and novel effective wound dressings that decrease wound infections are essential for clinical application. In this study, a multifunctional hydrogel was engineered based on polyvinyl alcohol (PVA), polydopamine (PDA), and porous silica (PSi), loaded with berberine chloride (Bbn), for the treatment of MRSA-infected wounds. The PVA@PSi/PDA-Bbn hydrogel was fabricated via sol-gel and self-polymerization methods, forming a porous, adhesive, and photothermal-responsive matrix. The hydrogel demonstrated excellent swelling behavior, mechanical strength, and sustained Bbn release under NIR irradiation. The efficacy of the hydrogels was evaluated through antibacterial activity, pathological assessments, and the expression of key proteins involved in cell proliferation and wound healing. These proteins included cyclin D1, c-Myc, Max, FGF-1, β-catenin, and WNT-1, and COL1A. The physicochemical properties of the hydrogels confirmed their successful synthesis and suitability for biomedical applications. The results for swelling, rheological properties, adhesive strength, safety, and antibacterial activity confirmed the hydrogels to be appropriate. The PVA@PSi40/PAD-Bbn hydrogel could enhance the healing process through its antibacterial activity and modulation of the expressions of cyclin D1, c-Myc, Max, FGF-1, β-catenin, and COL1-A. In sum, PVA@PSi40/PAD-Bbn could compete with the mupirocin ointment® and accelerate the MRSA-wound healing process. It showed better performance than conventional treatments by providing controlled drug release, strong tissue adhesion, and effective MRSA inhibition to promote wound healing.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"177 ","pages":"Article 214418"},"PeriodicalIF":6.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144722952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A customizable micropatterned platform for osteosarcoma spheroid generation, imaging, and drug screening","authors":"Maria Veronica Lipreri ,&nbsp;Marilina Tamara Totaro ,&nbsp;Ilaria Raimondi ,&nbsp;Margherita Cortini ,&nbsp;Nicola Baldini ,&nbsp;Sofia Avnet","doi":"10.1016/j.bioadv.2025.214419","DOIUrl":"10.1016/j.bioadv.2025.214419","url":null,"abstract":"<div><div>Spheroids are three-dimensional cell clusters that serve as reliable in vitro models for cancer drug screening, mimicking tumor microarchitecture and chemoresistance. Despite their potential, current spheroid culture systems lack essential but highly challenging features, such as automatic real-time imaging, treatment response assessment, and detailed live image capturing without disturbing the spheroid structure. To address these challenges, we developed a custom culture dish with a micro-patterned agarose structure, fabricated from a 3D-printed mold. This innovative tool facilitates spheroid growth and immobilization, enabling automated high-throughput imaging and data collection. It allows the microscope objective to approach the spheroid closely (within micrometers) while it floats in the culture medium, in a mapped position. Furthermore, the platform is compatible with several imaging systems, including standard, confocal and dual-photon microscopy. We successfully demonstrated the effectiveness of our platform by culturing and treating osteosarcoma spheroids with different concentrations of a standard chemotherapeutic agent and by capturing confocal images of extracellular matrix antigens in live spheroids. Additionally, we showed that the platform is compatible with viability and metabolic assays (e.g. Alamar blue and acid phosphatase), with minimal reagent consumption and cost-effective, simultaneous imaging-based assays.</div><div>In conclusion, we propose an innovative platform for the study of tumor spheroids and other three-dimensional cellular structures, which allows tracking of size, shape, antigen expression, viability, and indirect monitoring of cell count over time. This advancement enhances our capacity to conduct in-depth investigations of cell behavior and therapeutic responses, contributing significantly to cancer research and drug development.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"177 ","pages":"Article 214419"},"PeriodicalIF":5.5,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring bioactive bone cement pastes: A promising solution for orthopedic surgeries","authors":"Afsaneh Jahani ,&nbsp;Mehrnoush Nakhaei ,&nbsp;Ali Moradi ,&nbsp;Mohammad Hossein Ebrahimzadeh ,&nbsp;Mahnaz sadat Mirbagheri ,&nbsp;Azar Gharib ,&nbsp;Fatemeh Koohzad ,&nbsp;Nafiseh Jirofti","doi":"10.1016/j.bioadv.2025.214416","DOIUrl":"10.1016/j.bioadv.2025.214416","url":null,"abstract":"<div><div>Bone cement is a biomaterial that has been used effectively in orthopedic surgery for almost 50 years. The cement hardens after injection in the defect site without needing any external agent. Bone cement is commonly used in implant fixation, joint replacement, trauma surgery, osteoporosis management, and antibiotic delivery. Since the bone anatomy of patients is different, the final hardening of bone cement in the body corrects the abnormalities and gaps between the implant and the bone. The most universal injectable bone cements are based on ceramic or polymer compounds. The chemical composition of ceramic cement is similar to that of bone and hardens by chemical reaction, while polymerization promotes hardening in polymer-based bone cement. Despite the significant progress of bone cement in orthopedic surgery, its limitations and accompanying considerations must be acknowledged. Long-term degradation potentially leads to loosening of the implant and an exothermic reaction during hardening, leading to tissue necrosis. To address these limitations various approaches have been continuously evaluated. The major objective of this review is to comprehensively evaluate ceramic and polymer-based bone cement as a promising approach in orthopedic surgery. Adequate knowledge can lead to the induction of strategies to improve the performance of bone cement.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"177 ","pages":"Article 214416"},"PeriodicalIF":5.5,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An early rapamycin-releasing nerve wrap with dual function for nerve regeneration and adhesion prevention","authors":"Naiyu Wang ,&nbsp;Shiqi Kang ,&nbsp;Qi Zeng ,&nbsp;Wu Xu ,&nbsp;Yuanyuan Zhao ,&nbsp;Xudong Shi ,&nbsp;Ruijun Li","doi":"10.1016/j.bioadv.2025.214411","DOIUrl":"10.1016/j.bioadv.2025.214411","url":null,"abstract":"<div><div>Limited ability of nerve regeneration and the formation of nerve adhesions result to poor outcomes of peripheral nerve injury (PNI) following surgical nerve repair. Currently, there is no efficient therapy for the improvement of functional recovery after surgical repair. Rapamycin (Rapa) has been found useful for promoting nerve regeneration through removing cellular debris at the early stages after PNI. However, its lower oral bioavailability and significant adverse effects limit its clinical application. In this study, we developed a biocompatible nerve wrap based on biocompatible monomethoxy poly (ethylene gly<em>co</em>l)-poly (lactic-<em>co</em>-glycolic acid) (mPEG-PLGA) for the local delivery of Rapa. The Rapa-mPEG-PLGA nerve wrap exhibited a rough surface and good hydrophilicity with strong mechanical strength. In vitro release studies revealed an initial rapid phase within the first 8 days, followed by a stable release about 10 days. The released Rapa remained its bioactivity to induce autophagy and promote cell migration. In a rat model of sciatic nerve transection and immediate repair, the implantation of Rapa-mPEG-PLGA nerve wrap significantly enhanced axonal regeneration and myelination. Furthermore, the nerve wraps effectively prevented the occurrence of nerve adhesions. Improved functional recovery was observed with Rapa-mPEG-PLGA membrane wrap post-surgery 4 weeks and 8 weeks. In summary, the Rapa-loaded mPEG-PLGA nerve wrap promoted nerve regeneration by releasing Rapa at the early stage after injury, and acted as a physical barrier to reduce nerve adhesions. This local dual-action strategy is a promising candidate for clinical translation in the treatment of PNI following surgical repair.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"177 ","pages":"Article 214411"},"PeriodicalIF":5.5,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Poly-L-lactic acid nanofiber/polyamidoamine composite hydrogel as novel strategy for in vitro neuroregeneration and neuroprotection","authors":"Sofia Treccani ,&nbsp;Irene Bonadies ,&nbsp;Paolo Ferruti ,&nbsp;Jenny Alongi ,&nbsp;Enrico Scarpa ,&nbsp;Paola Laurienzo ,&nbsp;Maria Grazia Raucci ,&nbsp;Ines Fasolino ,&nbsp;Elisabetta Ranucci","doi":"10.1016/j.bioadv.2025.214415","DOIUrl":"10.1016/j.bioadv.2025.214415","url":null,"abstract":"<div><div>A purposely designed PLLA/H-AGMA₂₀ composite hydrogel incorporating an electrospun poly-L-lactic acid (PLLA) mat in a cell-adhesive polyamidoamine hydrogel (H-AGMA₂₀) was investigated as a novel biomaterial with neuroregenerative and neuroprotective properties. A PLLA mat was first functionalized by exposure to ethylenediamine vapors, introducing amine functions through ester group aminolysis, and then impregnated with an α,ω-acrylamide-terminated oligomer (AGMA₂₀) aqueous solution. The system was finally cured through UV-induced radical polymerization. The resulting H-AGMA₂₀ matrix was covalently grafted onto the PLLA nanofibers since a portion of the AGMA₂₀ acrylamide terminals reacted with their surface amine groups, leading to the soft and pliable PLLA/H-AGMA₂₀ composite. Biological investigations performed on preneuronal and immune cell lines demonstrated that PLLA/H-AGMA₂₀ exhibits promising neuroregenerative properties, effectively promoting both cell proliferation and neuronal differentiation. In addition, the composite demonstrated significant neuroprotective effects in vitro, counteracting neurotoxin 1-methyl-4-phenylpyridinium (MPP⁺) and proinflammatory agent lipopolysaccharide (LPS)-induced cytotoxicity. These protective results appear to be mediated by modulation of inflammatory pathways, highlighting the combined capacity of biomaterial to support neuronal development while reducing neuroinflammatory damage.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"177 ","pages":"Article 214415"},"PeriodicalIF":5.5,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered PDMS microtopography: Precision regulation of tendon stem cell behavior for tendon regeneration","authors":"Ya-Jing YE,&nbsp;Ya-Bo HOU,&nbsp;Zi-Xu ZHAO,&nbsp;Chang-Xing LI,&nbsp;Da-Chuan YIN,&nbsp;Dan-Bo SU","doi":"10.1016/j.bioadv.2025.214409","DOIUrl":"10.1016/j.bioadv.2025.214409","url":null,"abstract":"<div><div>Microtopography critically regulates tendon stem cell (TSC) migration for repair, yet its precise roles remain unclear. We engineered polydimethylsiloxane (PDMS) scaffolds with precisely defined micron-scale pits and columns (diameters 7–21 μm, spacings 13–47 μm) via ultraviolet lithography and etching. High-throughput screening to promote TSC viability identified six optimal topographies: three pit-based topographies (diameters/spacings: 7 μm/47 μm, 11 μm/18 μm, 21 μm/26 μm) and three column-based topographies (diameters/spacings: 8 μm/14 μm, 13 μm/13 μm, 18 μm/18 μm). Strikingly, TSCs exhibited distinct mechanoresponses: bypassing pits while wrapping around columns, with both inducing cytoskeletal alignment (40 % reduced orientation angle, 2-fold increased polarization compared to smooth substrates). Live-cell tracking revealed migration distance correlated with filopodia density, and crucially, migration rate peaked at a specific topological area proportion (AP ≈ 0.1). Critically, pits induced filopodia branching, whereas columns triggered pseudopodial wrapping and substrate deformation, revealing topology-specific mechanotransduction pathways. This study establishes microtopography as a potent designer signal for TSC mechanobiology and provides the quantitative principle for fabricating tendon-regenerative scaffolds with tailored topological gradients, offering a novel strategy to enhance repair efficiency.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"177 ","pages":"Article 214409"},"PeriodicalIF":5.5,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of the physical properties and biocompatibility assessments of low nickel stainless steel loaded with different graphene oxide content for safe biomedical applications","authors":"Doaa A. Abu Muslim ,&nbsp;Amal S. Shahat ,&nbsp;A.B. El Basaty ,&nbsp;A. Hassen ,&nbsp;A. Abou Elfadl ,&nbsp;Ahmed I. Ali ,&nbsp;A. Tayel","doi":"10.1016/j.bioadv.2025.214417","DOIUrl":"10.1016/j.bioadv.2025.214417","url":null,"abstract":"<div><div>The mechanical properties and biological responses are critical factors in evaluating surgical implants and medical devices. This study investigates the structure, mechanical performance, oxidative stress, proinflammatory cytokines, and histopathological outcomes of low-nickel stainless steel with varying graphene oxide (GO) additive concentrations. The results revealed that increasing the GO content decreases the material's density. Notably, the material containing 0.5 wt% GO demonstrated superior mechanical properties, including enhanced Young's modulus, shear modulus, and bulk modulus. Biocompatibility was assessed through in vivo studies using albino rats, with 36 animals divided into six groups. Oxidative stress levels showed no significant differences between the groups, irrespective of the GO concentration. Similarly, the assessment of proinflammatory cytokines TNF-α and IL-6 indicated no statistically significant differences (<em>P</em> &gt; 0.05), except for the group treated with low-nickel stainless steel powder (S0), which exhibited a slight variation in inflammatory markers. Histopathological examination of knee joints post-sacrifice revealed normal tissues and joints across all groups except for group S0, which showed mild edema. These findings suggest that incorporating GO into low-nickel stainless steel enhances its mechanical properties and confirms its potential as a biologically safe material for implants and medical devices.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"177 ","pages":"Article 214417"},"PeriodicalIF":5.5,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}