Materials Science & Engineering C-Materials for Biological Applications最新文献

{"title":"Microfluidic-engineering Prussian blue hydrogel microspheres for enhanced osteoarthritis antioxidant therapy","authors":"Wangrui Peng ,&nbsp;Jie Lan ,&nbsp;MeeiChyn Goh ,&nbsp;Meng Du ,&nbsp;Zhiyi Chen","doi":"10.1016/j.bioadv.2025.214345","DOIUrl":"10.1016/j.bioadv.2025.214345","url":null,"abstract":"<div><div>Osteoarthritis (OA), a degenerative joint disorder and leading cause of global disabilty, imposes substantial societal and familial burdens. Current antioxidant therapies for OA are hindered by poor targeting and transient efficacy, failing to address the excessive reactive oxygen species (ROS)-driven pathogenesis. Herein, we innovatively integrate Prussian blue (PB) nanozymes with alginate-hyaluronic acid (HA) hydrogel microspheres through microfluidic engineering, creating the injectable AlgHA@PB platform that synergizes dual therapeutic mechanisms: ROS scavenging and oxygen generation via PB nanozymes, and sustained intra-articular retention and mechanical compatibility enabled by the hydrogel microsphere architecture. In vitro studies demonstrated that AlgHA@PB scavenged all of intracellular ROS while continuously releasing oxygen within. In a rat OA model, AlgHA@PB exhibited prolonged joint retention and reduced cartilage degeneration. Critically, the microspheres demonstrated a stable friction coefficient, enabling smooth intra-articular motion without mechanical irritation. This study establishes AlgHA@PB as a multifunctional OA therapeutic platform that integrates antioxidative defense, anti-inflammatory action, and biomechanical compatibility. The microfluidic-engineered design ensures scalable production, aligning with clinical translation requirements.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"176 ","pages":"Article 214345"},"PeriodicalIF":5.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound - assisted microfluidics based microbubble mediated synthesis of nanoparticle - reinforced dual porous scaffolds for tissue regeneration","authors":"Aditya Teja Guduru ,&nbsp;Dhiraj Bhatia ,&nbsp;Mohan Edirisinghe ,&nbsp;Sameer Dalvi","doi":"10.1016/j.bioadv.2025.214347","DOIUrl":"10.1016/j.bioadv.2025.214347","url":null,"abstract":"<div><div>The development of biomimetic scaffolds with optimized porosity and mechanical properties is critical for tissue regeneration applications. This study aimed at production of nanoparticle reinforced dual porous scaffolds using a combination of ultrasound and microfluidics. Microfluidic T-junction device helped to achieve uniform primary pores through microbubble generation while ultrasound facilitated the fragmentation of microbubbles, resulting in formation of smaller secondary pores. The primary pores helped enhance nutrient and oxygen supply throughout the scaffold while the secondary pores provided a high surface area for cellular adhesion and cell distribution. The hierarchical pore size distribution was confirmed using Confocal microscopy and Scanning electron microscopy (SEM). Mechanical testing performed using a Universal Testing Machine (UTM) confirmed that the mechanical strength of the scaffolds closely matches to that of biological soft tissues. In vitro assays performed on the scaffolds using Human Embryonic Kidney (HEK 293) cells revealed enhanced cellular proliferation and uniform distribution of cells in scaffolds. The results suggested that synthesized scaffolds match physicochemical, mechanical, and biological properties of the native human tissues and can be used for tissue regeneration applications.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"176 ","pages":"Article 214347"},"PeriodicalIF":5.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144090673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lyophilised reservoirs in combination with hydrogel-forming microarray patches for transdermal delivery of isoniazid and pyridoxine hydrochloride","authors":"Qonita Kurnia Anjani ,&nbsp;Aaron R.J. Hutton ,&nbsp;Akmal Hidayat Bin Sabri ,&nbsp;Febri Annuryanti ,&nbsp;Helen O. McCarthy ,&nbsp;Ryan F. Donnelly","doi":"10.1016/j.bioadv.2025.214343","DOIUrl":"10.1016/j.bioadv.2025.214343","url":null,"abstract":"<div><div>Tuberculosis remains a major global health concern, presenting as either active disease or latent infection, the latter carrying a risk of activation, particularly in immunocompromised individuals. Prolonged isoniazid monotherapy is the standard preventive treatment, often supplemented with pyridoxine to mitigate isoniazid-induced pyridoxine depletion, as recommended by the US Centers for Disease Control and Prevention. This present study investigates an alternative transdermal approach using hydrogel-forming microarray patches (MAPs) incorporating lyophilised isoniazid and pyridoxine wafers. The MAPs were formulated with a novel poly(vinylpyrrolidone) and poly(vinyl alcohol) hydrogel, supplemented with sorbitol and adipic acid. <em>In vitro</em> studies demonstrated that approximately 15 % of isoniazid (8 mg) and 10 % of pyridoxine HCl (5 mg) permeated neonatal porcine skin over 24 h. In Sprague Dawley rats, MAPs provided significantly greater systemic exposure to isoniazid compared to oral administration (11,485 ± 1297 ng·mL⁻¹·day <em>vs.</em> 9538 ± 656 ng·mL⁻¹·day). A similar trend was observed for pyridoxine HCl, with MAPs yielding higher systemic exposure than the oral control (6118 ± 1185 ng·mL⁻¹·day <em>vs.</em> 823 ± 322 ng·mL⁻¹·day). These findings suggest that hydrogel-forming MAPs, which bypass first-pass metabolism and reduce hepatic exposure, hold promise as an effective alternative for the long-term management of latent tuberculosis.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"176 ","pages":"Article 214343"},"PeriodicalIF":5.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing compliance and extracellular matrix properties of tissue-engineered vascular grafts through pulsatile bioreactor culture","authors":"Angus Weekes ,&nbsp;Jordan W. Davern ,&nbsp;Nigel Pinto ,&nbsp;Jason Jenkins ,&nbsp;Zhiyong Li ,&nbsp;Christoph Meinert ,&nbsp;Travis J. Klein","doi":"10.1016/j.bioadv.2025.214346","DOIUrl":"10.1016/j.bioadv.2025.214346","url":null,"abstract":"<div><div>Biofabrication techniques represent a promising avenue for the production of small diameter vascular grafts. However, while current tissue-engineered vascular grafts (TEVGs) fulfil certain functional requirements of native blood vessels, most exhibit very poor mechanical compliance, directly reducing patency <em>in vivo</em>. Here, highly compliant TEVGs were cultured in a dynamic pulsatile bioreactor which ensured enhanced compliance, using biomimetic melt electrowritten (MEW) tubular scaffolds as substrates for tissue growth. Through 6-week <em>in vitro</em> culture, we investigated differences in extracellular matrix (ECM) production and mechanical performance of TEVGs cultured with placental mesenchymal stem cells (MSCs) and smooth muscle cells (SMCs) in static and dynamic conditions. Pulsatile stimulation successfully maintained the high compliance (12.4 ± 0.8 % per 100 mmHg) of our biomimetic scaffolds, substantially greater than existing small diameter grafts. Dynamic TEVGs demonstrated physiologically relevant burst pressure (1125 ± 212 mmHg) and suture pull-out force (3.0 ± 0.4 N), while also accumulating greater ECM components than static TEVGs. To assess off-the-shelf suitability, grafts were decellularized and lyophilised to produce d-TEVGs, which exhibited negligible loss of mechanics or ECM integrity. Finally, rehydrated d-TEVGs were seeded with endothelial cells <em>in vitro</em>, with an intimal endothelial lining forming after 7 days. These findings demonstrate the production of TEVGs with specifically engineered mechanical compliance which has been maintained by dynamic <em>in vitro</em> culture, supporting continued work toward biofabrication of the next generation of vascular grafts.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"175 ","pages":"Article 214346"},"PeriodicalIF":5.5,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inactivation of antibiotic resistant bacteria by ruthenium-doped carbon dots capable of photodynamic generation of intracellular and extracellular reactive oxygen species","authors":"Weibo Xia ,&nbsp;Jia Shan ,&nbsp;Vladyslav Lutsenko ,&nbsp;Zhang Cheng ,&nbsp;Yu Liu ,&nbsp;Jinjia Xu ,&nbsp;Shiqiang Yu ,&nbsp;Zheng Peng ,&nbsp;Heyang Yuan ,&nbsp;Wenfei Hu","doi":"10.1016/j.bioadv.2025.214344","DOIUrl":"10.1016/j.bioadv.2025.214344","url":null,"abstract":"<div><div>Wound infections caused by methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) present a significant challenge to wound healing. This has motivated the development of novel antibiotic-free agents. In this study, ruthenium-doped carbon dots (Ru-CDs) with photodynamic antibacterial activity were synthesized to treat MRSA-infected skin wounds. The Ru-CDs were prepared <em>via</em> a hydrothermal method using Ru-Aphen as the nitrogen source and citric acid as the carbon source, resulting in uniform spherical nanoparticles with an average size of 2.7 ± 0.8 nm. Singlet oxygen generation was observed when the Ru-CDs were exposed to light. <em>In vitro</em> experiments showed concentration- and light-dependent antibacterial activity of the Ru-CDs against MRSA, with 99.9 % bacterial reduction when treated with 100 μg/mL Ru-CDs under light for 10 min. A significant level of intracellular ROS was observed, and microscopy confirmed bacterial membrane disruption. Biocompatibility tests showed no significant toxicity, and <em>in vivo</em> studies on rabbit wound models demonstrated effective antibacterial activity under light conditions and enhanced wound healing compared to controls. The results collectively highlight the potential of Ru-CDs as an antibiotic-free agent for treating antibiotic resistant bacterial infections through photodynamic generation of extracellular ROS and induction of intracellular ROS.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"176 ","pages":"Article 214344"},"PeriodicalIF":5.5,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AuPt-loaded Fe-N-C flower-like nanocascade reactor with self-supplied reaction substrates and multiple enzyme activities for the study of hypoxic tumors","authors":"Yang Guo ,&nbsp;Xinyu Gao ,&nbsp;Fang He ,&nbsp;Sihan Wang ,&nbsp;Jingting Li ,&nbsp;Fan Yang ,&nbsp;Zihan Zhou ,&nbsp;Changsong Dai ,&nbsp;Zhaohui Wen","doi":"10.1016/j.bioadv.2025.214341","DOIUrl":"10.1016/j.bioadv.2025.214341","url":null,"abstract":"<div><div>When nano-enzymes are used in tumor therapy research, they usually exhibit only a single enzyme activity, which limits their therapeutic effect in hypoxic tumors. This paper designs a hyaluronic acid (HA)-modified Au-Pt-Fe trimetallic flower-like nanomaterial for tumor cascade catalysis integrated with low-temperature photothermal therapy. GOx activity exhibited by AuPt nanoparticles converts glucose in tumor cells into gluconic acid and H₂O₂. This process not only deprives tumor cells of their energy source but also produces H₂O₂. Iron single-atom nanomaterials can exhibit excellent CAT and POD activities, catalyzing H₂O₂ reactions in tumor cells. Among them, a portion of H₂O₂ is converted to O₂ by the activity of nanozyme CAT, which is used to alleviate tumor tissue hypoxia and serve as a substrate for the GOx-like activity reaction of AuPt NPs. The H₂O₂ is continuously produced by the glucose catalytic reaction, and a portion of it can further decompose into toxic <img>OH through POD activity, contributing to tumor cell destruction. The glucose-H₂O₂-O₂ cycle reaction is realized, that is, the self-supply of H₂O₂ and O₂ in the catalytic process within tumor cells. The introduction of AuPt nanoparticles enhances the photothermal conversion ability of the material. By using near-infrared light irradiation, mild-temperature photothermal therapy (m-PTT) can be achieved, which further improves the treatment effect while protecting surrounding tissues. This work expands the application of multi-metal nanomaterials in cascade catalysis combined with low-temperature photothermal therapy, providing a novel strategy for integrated tumor treatment.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"176 ","pages":"Article 214341"},"PeriodicalIF":5.5,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered bioactive glass-chitosan hybrid for dual tissue and bone regeneration multifunctional healing","authors":"Yu-Chien Lin ,&nbsp;Susaritha Ramanathan ,&nbsp;Huey-Yuan Wang ,&nbsp;Ying-Chun Lin ,&nbsp;Wai-Ching Liu ,&nbsp;Julian R. Jones ,&nbsp;Nam-Joon Cho ,&nbsp;Chih-Chien Hu ,&nbsp;Ren-Jei Chung","doi":"10.1016/j.bioadv.2025.214340","DOIUrl":"10.1016/j.bioadv.2025.214340","url":null,"abstract":"<div><div>The treatment of complex wounds, particularly those arising from conditions such as diabetes or trauma, presents a significant clinical challenge. These wounds often necessitate long-term care for soft tissue and bone repair, creating an urgent need for multifunctional wound dressings. This study introduces a chitosan-silica hybrid dressing enhanced with bioactive glass (BG), specifically designed to address this need. The hybrid material achieves molecular bonding through coupling agents, with the organic chitosan component providing swelling and hemostatic effects, while the inorganic silica and BG release ions (Si⁴⁺, Ca²⁺), promoting tissue regeneration and bone healing. With a controlled degradation profile (lasting 3 to 6 months <em>in-vitro</em>), the dressing is ideal for chronic wound management. Experimental results demonstrate excellent biocompatibility, no inflammatory response, and strong hemostatic properties due to the positively charged chitosan and cations. Adding BG significantly enhances bone regeneration, positioning this chitosan-silica hybrid as a promising solution for the simultaneous repair of soft tissue and bone.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"176 ","pages":"Article 214340"},"PeriodicalIF":5.5,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alginate-based hydrogels for sustained antimicrobial peptide delivery to enhance wound healing in diabetes","authors":"Jessica Da Silva ,&nbsp;Ermelindo C. Leal ,&nbsp;Ana Gomes ,&nbsp;Paula Gomes ,&nbsp;Daniela Calheiros ,&nbsp;Teresa Gonçalves ,&nbsp;Eugénia Carvalho ,&nbsp;Eduardo A. Silva","doi":"10.1016/j.bioadv.2025.214337","DOIUrl":"10.1016/j.bioadv.2025.214337","url":null,"abstract":"<div><div>Diabetic foot ulcers (DFUs) are the leading cause of non-traumatic amputations, and its efficient management remains a clinical challenge, particularly in treating severe infections. Current treatment strategies often fail to address the multifactorial nature of DFUs. Combining antimicrobial peptides (AMPs) with the intrinsic properties of alginate hydrogels offers a promising solution for handling the complex etiology of DFUs. In this study, we designed alginate-based hydrogels for delivery of AMPs, namely the AMPs human β-defensin 2 (hBD-2) and PP4-3.1, to enhance diabetic wound healing. The hydrogels exhibited high storage modulus, low swelling ratio, and a nanometric porous structure, enabling sustained AMP release for over three days. Rheology analyses further confirmed their stability across pH 6 to 8. <em>In vitro</em>, hBD-2 hydrogels displayed excellent biocompatibility and promoted better cell migration than PP4-3.1 hydrogels, for up to 48 h. Thus, hBD-2 hydrogels were used in a streptozotocin-induced diabetic mouse model of wound healing. The hBD-2 hydrogels significantly accelerated wound closure and improved wound maturation, enhancing re-epithelialization and tissue remodeling, compared to controls. Furthermore, hBD-2 hydrogels reduced the microbial load from the wounds and attenuated inflammation at the wound site by decreasing the number of M1-like macrophages, M1/M2 ratio, and CD3⁺ cells. Lastly, a pro-reparative environment was promoted through a decrease in reactive oxygen species (ROS) levels, and an increase in neovascularization and collagen deposition. Altogether, these findings suggest that hBD-2 alginate hydrogels hold promise as a novel therapeutic option for managing DFUs, offering a combined anti-inflammatory, ROS-scavenging and tissue-regenerative effect.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"175 ","pages":"Article 214337"},"PeriodicalIF":5.5,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143936522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of selenium-doped black phosphorus nanosheets wrapped with biomimetic tumor cell membrane for prostate cancer immunotherapy","authors":"Xingjian Yan ,&nbsp;Han Dong ,&nbsp;Liyin Gao ,&nbsp;Mengqi Liu ,&nbsp;Chunxi Wang","doi":"10.1016/j.bioadv.2025.214339","DOIUrl":"10.1016/j.bioadv.2025.214339","url":null,"abstract":"<div><div>Prostate cancer (PCa) is commonly considered a “cold tumor” due to its immunosuppressive microenvironment. Cold tumors are typically identified by the absence of T-cell infiltration within the tumor, while other immune populations and myeloid cells can be observed in these tumors. To achieve light-heat combined immunotherapy checkpoint inhibitor treatment for castration-resistant prostate cancer, we aimed to transforming “cold tumors” into “hot tumors”. We designed and synthesized a two-dimensional material, selenium-doped black phosphorus (BP), to enhance the photothermal conversion efficiency, and formed Se@BPNSs by liquid-phase exfoliation. To address the issue of enhanced permeability and retention effect, and to achieve efficient targeting, we coated the Se@BPNSs with RM-1 cell membrane derived from mouse prostate cancer cells. By injecting a certain dose of Se@BPNSs into the tumor and irradiating with a 808 nm laser, the Se@BPNSs converted light energy into heat to kill tumor cells at high temperatures while releasing antigens captured by dendritic cells. In addition, we combined the immunotherapy checkpoint inhibitor anti-PD1 to enhance the immune response and promote immune cell infiltration. The successful preparation of Se@BPNSs was verified through material characterization, cell-level and animal-level experiments, and the antitumor effect was meanwhile verified, which further provided guidance for prostate cancer treatment by photothermal synergistic immunotherapy.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"176 ","pages":"Article 214339"},"PeriodicalIF":5.5,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Matrix-bound nanovesicles recapitulate tissue-specific angiogenic properties of parent extracellular matrix with distinct miRNA profiles","authors":"Hee-Woong Yun ,&nbsp;Mijin Kim ,&nbsp;Dong Il Shin ,&nbsp;Hyeon Jae Kwon ,&nbsp;In-Su Park ,&nbsp;Do Young Park ,&nbsp;Byoung-Hyun Min","doi":"10.1016/j.bioadv.2025.214338","DOIUrl":"10.1016/j.bioadv.2025.214338","url":null,"abstract":"<div><div>Decellularized extracellular matrix (dECM) exhibits tissue-specific pro- or anti-angiogenic effects. Previous studies have demonstrated that matrix-bound nanovesicles (MBVs) act as key bioactive components of dECM, replicating various biological functions such as anti-inflammatory and immunomodulatory effects. Building on this evidence, this study hypothesized that MBVs derived from cartilage and small intestinal submucosa (SIS) modulate angiogenesis through the selective packaging of miRNAs. Cartilage-derived MBVs (cMBVs) and SIS-derived MBVs (sMBVs) were isolated, characterized, and analyzed for their miRNA profiles using RNA sequencing and RT-qPCR validation. The interactions between MBVs and human umbilical vein endothelial cells (HUVECs) were assessed by examining proliferation, adhesion, migration, and tube formation in comparison to the parent ECM. Angiogenic modulation was further evaluated using a mouse Matrigel plug assay and a rabbit corneal neovascularization (NV) model. Our results demonstrated that anti-angiogenic miRNAs (e.g., miR-140-3p, miR-455-5p, and miR-148a-5p) were predominant in cMBVs, suppressing endothelial cell activity and angiogenesis, while pro-angiogenic miRNAs (e.g., miR-143-3p, miR-181a, and miR-21-5p) were prevalent in sMBVs, enhancing vessel formation. In vivo, cMBVs significantly inhibited vascular invasion and neovessel formation, whereas sMBVs promoted angiogenesis in both models. These findings confirm that MBVs reflect the tissue-specific angiogenic regulatory functions of their parent ECM, and highlight their potential as therapeutic tools for targeted modulation of angiogenesis in regenerative medicine and tissue engineering.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"175 ","pages":"Article 214338"},"PeriodicalIF":5.5,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}