Engineered small-diameter vascular graft with tailored degradation profile transform into a living blood vessel after implantation in a murine model

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications Pub Date : 2025-09-30 DOI:10.1016/j.bioadv.2025.214533

Do Wan Kim , Mukhammad Kayumov , Reverien Habimana , Jiae Seong , YeongEun Jo , Hwa-Jin Cho , Obiweluozor Francis Onyekachi , In Seok Jeong

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引用次数: 0

Abstract

Current vascular replacements available on the market often require frequent interventions. In situ tissue engineering (TE), which utilizes biodegradable grafts that transform into autologous vascular tissue at the implantation site, presents a promising solution to these limitations. In this study, electrospun small-diameter scaffolds (2 mm ø) composed of polycaprolactone (PCL) and polydioxanone (PDO), reinforced with a 3D-printed PCL anti-kinking coil, were implanted in a rat abdominal aortic replacement model. Grafts were explanted after 3 and 12 months, and the results were compared with native rat aortic vessels of similar age. The implanted grafts were evaluated for patency, extracellular matrix (ECM) formation, degradation, and remodeling. At 3 months, the grafts were partially resorbed and replaced by vascular neo-tissue due to the degradation of PDO nanofibers. By 12 months, nearly complete graft resorption was observed, with the newly formed vessel exhibiting structural and functional characteristics similar to the native aorta, including elastin deposition, contractile marker expression, and endothelial lining formation. No cases of dilation, rupture, or aneurysmal formation were observed. Patency of the implanted grafts was confirmed by OCT (≈100 %), comparable to native controls.

This work employed a small animal model to illustrate the transformation of a synthetic, biodegradable vascular scaffold into a functioning, compliant neo-vessel in a rat aortic replacement.

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在植入小鼠模型后，具有定制降解特征的工程化小直径血管移植物转化为活血管

目前市场上可用的血管置换术通常需要频繁的干预。原位组织工程（TE）利用可生物降解的移植物在植入部位转化为自体血管组织，为解决这些限制提供了一个有希望的解决方案。本研究将由聚己内酯（PCL）和聚二恶酮（PDO）组成的小直径（2 mm ø）静电纺丝支架，用3d打印PCL抗扭结线圈加固，植入大鼠腹主动脉置换模型。分别在3个月和12个月后移植物外植，并与相近年龄的天然大鼠主动脉血管进行比较。评估移植的移植物的通畅、细胞外基质（ECM）的形成、降解和重塑。3个月时，由于PDO纳米纤维的降解，移植物被部分吸收并被血管新生组织取代。到12个月时，观察到移植物几乎完全吸收，新形成的血管具有与天然主动脉相似的结构和功能特征，包括弹性蛋白沉积、收缩标志物表达和内皮内膜形成。没有观察到扩张、破裂或动脉瘤形成的病例。OCT证实移植物通畅（≈100%），与天然对照相当。这项工作采用了一个小动物模型来说明在大鼠主动脉置换术中将合成的、可生物降解的血管支架转化为功能良好的、顺应性的新血管。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Materials Science & Engineering C-Materials for Biological Applications 工程技术-材料科学：生物材料

CiteScore

17.80

自引率

0.00%

发文量

501

审稿时长

27 days

期刊介绍： Biomaterials Advances, previously known as Materials Science and Engineering: C-Materials for Biological Applications (P-ISSN: 0928-4931, E-ISSN: 1873-0191). Includes topics at the interface of the biomedical sciences and materials engineering. These topics include: • Bioinspired and biomimetic materials for medical applications • Materials of biological origin for medical applications • Materials for "active" medical applications • Self-assembling and self-healing materials for medical applications • "Smart" (i.e., stimulus-response) materials for medical applications • Ceramic, metallic, polymeric, and composite materials for medical applications • Materials for in vivo sensing • Materials for in vivo imaging • Materials for delivery of pharmacologic agents and vaccines • Novel approaches for characterizing and modeling materials for medical applications Manuscripts on biological topics without a materials science component, or manuscripts on materials science without biological applications, will not be considered for publication in Materials Science and Engineering C. New submissions are first assessed for language, scope and originality (plagiarism check) and can be desk rejected before review if they need English language improvements, are out of scope or present excessive duplication with published sources. Biomaterials Advances sits within Elsevier''s biomaterials science portfolio alongside Biomaterials, Materials Today Bio and Biomaterials and Biosystems. As part of the broader Materials Today family, Biomaterials Advances offers authors rigorous peer review, rapid decisions, and high visibility. We look forward to receiving your submissions!