Microchemical Journal最新文献

{"title":"Label-free photoelectrochemical detection of carboxylesterase activity based on carboxylesterase-induced amide bond hydrolysis","authors":"Jun Chen,&nbsp;Jing Hu,&nbsp;Yuting Ding,&nbsp;Rong Liao,&nbsp;Chao Chen,&nbsp;Wenfang Deng,&nbsp;Yueming Tan,&nbsp;Qingji Xie","doi":"10.1016/j.microc.2025.114625","DOIUrl":"10.1016/j.microc.2025.114625","url":null,"abstract":"<div><div>Carboxylesterase (CE), a promising biomarker for hepatocellular carcinoma (HCC), demands rapid and precise detection for clinical diagnosis. However, current detection methods are hampered by significant technical challenges. This study presents a novel label-free photoelectrochemical (PEC) biosensor based on amide-bond interactions between photoactive materials to detect CE activity. We constructed a Cd_0.6Zn_0.4S/In₂O₃ heterojunction with optimized band alignment, which enabled efficient separation of photogenerated electron-hole pairs (electron lifetime as long as 0.127 s) and significantly enhanced photocurrent generation efficiency (incident photon-to-electron conversion efficiency (IPCE) reaching 15.9 %). In this biosensing system, Cd_0.6Zn_0.4S nanoparticles were covalently linked to In₂O₃ nanospheres via amide bonds crosslinking. Upon the introduction of CE, enzymatic hydrolysis of the amide bond induced the detachment of Cd_0.6Zn_0.4S from the electrode surface, leading to a proportional decrease in the photoanodic signal. Experimental results showed that the photocurrent response exhibited a linear relationship with the logarithm of CE activity within the range of 10⁻⁵ to 0.4 U L⁻¹, with a detection limit of 3.2 × 10⁻⁶ U L⁻¹. Furthermore, the proposed PEC detection platform exhibits high selectivity and good stability for the detection of CE activity in human serum samples, demonstrating a spike recovery of 94 % to 102 % for CE determination in human serum. This label-free strategy enables sensitive detection of CE by modulating interfacial charge transport, providing a simplified yet effective approach for the early diagnosis of hepatocellular carcinoma.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"216 ","pages":"Article 114625"},"PeriodicalIF":4.9,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144710989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of lactic acid sensor based on Pd@PPyC-Bi2S3 nanocomposites by linear sweep voltammetry","authors":"M. Faisal ,&nbsp;M.M. Alam ,&nbsp;Jahir Ahmed ,&nbsp;Muneera S.M. Al-Saleem ,&nbsp;Amjad E. Alsafrani ,&nbsp;Jari S. Algethami ,&nbsp;Jehan Y. Al-Humaidi ,&nbsp;Abdulkarim Albishri ,&nbsp;Farid A. Harraz ,&nbsp;Mohammed Muzibur Rahman","doi":"10.1016/j.microc.2025.114631","DOIUrl":"10.1016/j.microc.2025.114631","url":null,"abstract":"<div><div>The efficient and selective detection of lactic acid (LA) holds significant importance across the various sectors, such as clinical diagnostics, food quality monitoring, biomedical implementation, and environmental analysis. This study presents the development of an innovative electrochemical sensor probe utilizing Pd@PPyC-Bi₂S₃ nanocomposites (NCs) applied to a flat glassy carbon electrode (GCE) by PEDOT:PSS chemical binder for the precise and targeted detection of LA under ambient conditions. The Pd@PPyC-Bi₂S₃ NCs were ultrasonically synthesized and fully characterized by using various techniques, including X-ray Diffraction (XRD), Brunauer-Emmett-Teller (BET), Field Emission Scanning Electron Microscopy (FESEM), High-Resolution Transmission Electron Microscopy (HR-TEM), Ultraviolet–visible Spectroscopy (UV–vis), and X-ray Photoelectron Spectroscopy (XPS). The Pd@PPyC-Bi₂S₃ NCs/PEDOT:PSS/GCE demonstrated remarkable electro-catalytic performance for the oxidation of LA, featuring an extensive linear detection range (LDR; 1.0 ⁓ 70.0 μM), a lower limit of detection (LOD; 0.40 ± 0.02 μM), and higher sensitivity (248.48 μAμM⁻¹ cm⁻²). The fabricated Pd@PPyC-Bi₂S₃ NCs/PEDOT:PSS/GCE sensor probe exhibited exceptional selectivity when faced with typical interferents, rendering it appropriate for practical applications. Moreover, the developed electrochemical sensor was effectively utilized for the measurement of LA across the different real sample matrices, such as rabbit serum, rat serum, and human urine, demonstrating its practical validity. The facile synthesis, exceptional performance, and wide-ranging applicability of the Pd@PPyC-Bi₂S₃ NCs/PEDOT:PSS/GCE-based electrochemical sensor position it as a highly promising analytical probe for the sensitive and selective detection of LA, contributing to safety in healthcare and biomedical fields on a broad scale.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"216 ","pages":"Article 114631"},"PeriodicalIF":4.9,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144686937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon-coated UiO-66(Zr)@MoS2 for the electrochemical measurement of urea in sweat","authors":"Daidai Wang ,&nbsp;Ying Cao ,&nbsp;Moustafa Zahran ,&nbsp;Xinchun Liu ,&nbsp;Xiaoyue Hui ,&nbsp;Ahmed S. Abou- Elyazed ,&nbsp;Hui-Ying Qu ,&nbsp;Xiaolin Li","doi":"10.1016/j.microc.2025.114615","DOIUrl":"10.1016/j.microc.2025.114615","url":null,"abstract":"<div><div>This study reports a novel electrochemical sensor based on a metal-organic framework (MOF) derivative to measure urea in sweat. The MOF structure, UiO-66(Zr), was greenly synthesized using a solvent-free thermal method, functionalized with molybdenum disulfide (MoS₂), and then thermally treated to form carbon-coated UiO-66(Zr)@MoS₂. The MOFs were characterized by X-ray diffraction, an N₂ adsorption instrument, scanning electron microscopy, and an X-ray photoelectron spectrometer. Among the prepared MOFs, the carbon-coated UiO-66(Zr)@MoS₂ showed many crystal defects, providing more active sites required to improve the electrocatalytic activity, and high carbon content to enhance the electrical conductivity of the stainless steel (SS) electrode. The electrocatalytic activity of the MOFs was evaluated using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and square wave voltammetry (SWV). CV studies showed poor catalytic activity of bare SS electrode and its modifiers, UiO-66(Zr), carbon-coated UiO-66(Zr), and UiO-66(Zr)@MoS₂, towards urea (0.1 mM) oxidation. In contrast, the carbon-coated UiO-66@MoS₂ modifier exhibited a urea oxidation peak with a current value of 5 μA, confirming its high electrocatalytic activity. Compared to CV, SWV exhibited a higher oxidation peak current (7.5 μA). Additionally, the EIS showed a 38.4 % reduction in the electron transfer resistance after SS electrode modification with the carbon-coated UiO-66(Zr)@MoS₂. The SWV sensor exhibited lower detection and quantification limits of 0.4 and 1.4 μM, respectively. Additionally, it showed high selectivity in the presence of possible interfering substances, such as glucose, lactic acid, ascorbic acid, cortisol, and sodium chloride. Moreover, its efficacy for measuring urea in artificial sweat samples was proved with acceptable recovery values.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"216 ","pages":"Article 114615"},"PeriodicalIF":4.9,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144686938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential of Bacillus subtilis: innovative applications in energy harvesting and biosensing technologies","authors":"Sara Moradi ,&nbsp;Morteza Hosseini ,&nbsp;Elyor Berdimurodov","doi":"10.1016/j.microc.2025.114633","DOIUrl":"10.1016/j.microc.2025.114633","url":null,"abstract":"<div><div>The advancement of technology has significantly boosted the use of microorganisms in biotechnological applications. The term microbial fuel cells refer to systems that produce electrical energy by using microorganisms as biocatalysts, utilizing organic materials as substrates or nutrients for these microorganisms. Among the diverse types of microorganisms, <em>Bacillus subtilis</em>, a Gram-positive, rod-shaped bacterium, has attracted significant interest from researchers due to its remarkable characteristics, including spore and biofilm formation, metabolic versatility, signal amplification abilities, and electroactive effects. This review article examines the applications of <em>Bacillus subtilis</em> in two main domains of biotechnology. The first pertains to biosensing, where the bacterium's natural affinity for various substrates and its ability to produce specific biomolecules are leveraged for the detection of environmental pollutants, pathogens, and biomolecules such as glucose. In this review, biosensors based on <em>Bacillus subtilis</em> are classified according to the type of signal they produce, including electrochemical, luminescent, and colorimetric outputs, providing a structured overview of their mechanisms and applications. The second aspect relates to energy harvesting, where this bacterium is utilized to transform chemical energy into electrical energy, representing a promising approach for generating renewable and environmentally friendly power. In this review, energy harvesting applications are classified based on the bacterial form employed—such as spores, biofilms, or multispecies consortia—highlighting their roles in enhancing system stability, efficiency, and power output. The integration of novel nanomaterials significantly improves the functionality of these platforms, including the augmentation of energy generation in energy harvesting systems and the heightened selectivity and sensitivity in biosensors, resulting in the creation of highly efficient systems. This review paper elucidates the role of <em>Bacillus subtilis</em> and the rationale behind its extensive utilization in biotechnological applications. It classifies the primary applications of this bacterium in the biotechnology sector based on established criteria and presents recent studies within each category, thereby offering valuable insights and highlighting existing gaps for future research.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"216 ","pages":"Article 114633"},"PeriodicalIF":4.9,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144710986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3-(Bis(2-methyl-1H-indol-3-yl)methyl)benzene-1,2-diol (BID) as signal amplifiers in electrochemical DNA biosensors for Alexandrium minutum detection","authors":"Devika Nokarajoo,&nbsp;Siti Aishah Hasbullah,&nbsp;Nurul Huda Abd. Karim,&nbsp;Yook Heng Lee,&nbsp;Emma Izzati Zakariah","doi":"10.1016/j.microc.2025.114630","DOIUrl":"10.1016/j.microc.2025.114630","url":null,"abstract":"<div><div>Indole derivatives with strong electron-donating and electron-withdrawing functional groups hold significant promise in biological applications due to their exceptional ability to interact with DNA through non-covalent binding. Such features facilitate their functions in molecular biology, including studies of DNA binding, biosensor development, and drug design. In this study, an indole derivative, 3-(bis(2-methyl-1H-indol-3-yl)methyl)benzene-1,2-diol (<strong>BID</strong>), was synthesised and characterised as a new compound. The structure of <strong>BID</strong> was determined by CHNS elemental analysis, FTIR and NMR spectroscopy. For the first time, <strong>BID</strong> was used to enhance labelling in a DNA biosensor for the detection of <em>Alexandrium minutum</em> (<em>A. minutum</em>) DNA. A combination of UV–vis absorption, luminescence titrations, viscosity measurements, circular dichroism, and molecular docking procedures were used to explore the binding interaction of <strong>BID</strong> to <em>A. minutum</em> DNA. <strong>BID</strong> binds strongly to DNA through intercalation mode, indicating its potential for targeted applications. To evaluate the biosensing applications of <strong>BID</strong>, the aminated DNA probe was covalently immobilised on the poly(n-butyl acrylate-<em>co</em>-N-acryloxysuccinimide) (poly(nBA-<em>co</em>-NAS)) microspheres coated onto carbon screen-printed electrodes (SPE) with deposited gold nanoparticles. The response of the biosensor towards the target <em>A. minutum</em> DNA was measured using differential pulse voltammetry after the intercalation with <strong>BID</strong>. The biosensor demonstrated a broad linear range towards the target DNA of <em>A. minutum</em> from 1 × 10⁻⁵ to 1 × 10⁻¹⁵ M (R² = 0.9973) with a very low minimum detection limit of 3.33 × 10⁻¹⁷ M. Additionally, the biosensor retained 86 % of its initial response to toxic algae after 23 days of storage at 4 °C, demonstrating robust stability. Overall, these results highlight the effectiveness of <strong>BID</strong> as a labelling agent in DNA hybridisation assays and underscore its potential as a critical component for the sensitive and selective detection of <em>A. minutum</em> DNA in biosensing applications.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"216 ","pages":"Article 114630"},"PeriodicalIF":4.9,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facile fabrication of V2CTx MXene/In2O3 heterostructure for selective chlorine detection at room temperature","authors":"Guangji Zhang ,&nbsp;Ziwei Zhang ,&nbsp;Weixiao Hou ,&nbsp;Jifan Hu ,&nbsp;Kewei Zhang ,&nbsp;Zhigang Sun ,&nbsp;Jiangwei Ma","doi":"10.1016/j.microc.2025.114608","DOIUrl":"10.1016/j.microc.2025.114608","url":null,"abstract":"<div><div>Taking advantage of the poor solubility of MXene in tetrahydrofuran (THF), MXene is unable to compensate for its large surface energy in the non-solvated state. In₂O₃ nanoparticles can be assembled on the surface of V₂CT_x MXene via van der Waals interactions, which minimis the surface energy and forms heterostructures to stabilize the MXene. The surface and layers of V₂CT_x MXene exhibit a uniform dispersion of In₂O₃ nanoparticles, which effectively prevents the agglomeration of these nanoparticles as well as the structural collapse of MXene. The sensor based on V₂CT_x MXene/In₂O₃ (2:1) composite exhibits excellent gas response and stability to chlorine (Cl₂) gas at room temperature. The response value for Cl₂ at a concentration of 0.5 ppm is 4.11 %. The response time and recovery time of the sensor are 153 s and 527 s, respectively. Additionally, the integration of In₂O₃ with V₂CT_x MXene improves specific surface area of the material. The creation of an n-n junction between In₂O₃ and V₂CT_x MXene facilitates a higher rate of charge transfer, effectively resolving the problems of inadequate electrical conductivity and elevated operational temperatures.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"216 ","pages":"Article 114608"},"PeriodicalIF":4.9,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polydopamine-coated aptamer-modified gold nanoparticles for enhanced Pb2+ sensing via molecular imprinting","authors":"Tai Ye ,&nbsp;Xiuxiu Wu ,&nbsp;Caiwei Su,&nbsp;Mengyuan Huang,&nbsp;Long Bai,&nbsp;Mei Xue,&nbsp;Min Yuan,&nbsp;Hui Cao,&nbsp;Liling Hao,&nbsp;Fengqin Yin,&nbsp;Fei Xu","doi":"10.1016/j.microc.2025.114607","DOIUrl":"10.1016/j.microc.2025.114607","url":null,"abstract":"<div><div>The moderate recognition capability of the aptamer at the interface due to its unpredictable conformation. Methods to regulate the recognition conformation of aptamer is the essential to improve sensing performance. Herein, we proposed polydopamine layer to fix the lead ion (Pb²⁺)-binding conformation of aptamer (T30695) at the surface of gold nanoparticle. Through the ion imprinting process, the polydopamine coated aptamer modified gold nanoparticle (IIP) exhibited better binding affinity and kinetics than that of aptamer modified gold nanoparticle (Au-T30695). The polydopamine layer stabilized the conformation of aptamer was also confirmed through the molecular dynamic simulation. In addition, the peroxidase-like activity of gold nanoparticles was further increased through the specific binding between Pb²⁺ and aptamer, which catalyze hydrogen peroxide (H₂O₂) mediated oxidization of Amplex UltraRed (AUR). Under the optimum condition, the limit of detection of Pb²⁺ was estimated to be 37 pM. The proposed IIP exhibited excellent selectivity and anti interference in the presence of interferences ions. The satisfactory recoveries of proposed IIP in the spiked river water sample demonstrated its promising practical performance in complex environment. This study offers new insights into the design of nanozyme probes with both target specificity and enhanced catalytic performance.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"216 ","pages":"Article 114607"},"PeriodicalIF":4.9,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZIF-67 derived hollow HEDP-Co nanopolyhedra for electrochemical sensing of dopamine","authors":"Meng Lin,&nbsp;Yanjun Lyu,&nbsp;Pengfei Cao,&nbsp;Nan Wang,&nbsp;Chuanxu Yang","doi":"10.1016/j.microc.2025.114627","DOIUrl":"10.1016/j.microc.2025.114627","url":null,"abstract":"<div><div>For the sensitive and selective electrochemical detection of dopamine (DA), a hollow etidronic acid–cobalt nanopolyhedra (HEDP–Co) was synthesized using zeolitic imidazolate framework-67 (ZIF-67) as a template. ZIF-67 was etched by etidronic acid (HEDP) to form a hollow nanostructure, during which the released cobalt ions coordinated with HEDP to generate a complex. The formation and structure of the resulting hollow HEDP–Co were confirmed by transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS). A glassy carbon electrode (GCE) modified with the HEDP–Co nanomaterial was fabricated for the sensitive and accurate electrochemical detection of dopamine. Owing to the synergistic effect between HEDP and cobalt ions, the hollow nanostructure significantly enhanced the electrochemical response toward dopamine. In phosphate buffer solution, the developed sensor exhibited a linear response to dopamine in the concentration range of 0.01–1.50 mM, with a detection limit of 4.0 μM. Furthermore, the sensor demonstrated good selectivity, stability, and reproducibility, indicating its potential for practical applications in dopamine sensing.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"216 ","pages":"Article 114627"},"PeriodicalIF":4.9,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144686935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Near-infrared dye conjugated polysaccharide nanogel-based contrast agents for M2 macrophage-targeting photoacoustic imaging","authors":"Risako Miura ,&nbsp;Akio Tsutsumi ,&nbsp;Yu Kimura ,&nbsp;Kazunari Akiyoshi ,&nbsp;Teruyuki Kondo","doi":"10.1016/j.microc.2025.114618","DOIUrl":"10.1016/j.microc.2025.114618","url":null,"abstract":"<div><div>Cancer immunotherapy provides high anti-tumor effects with minimal side effects by harnessing the patient's immune systems. However, immunosuppressive tumors, called cold tumors, are unresponsive to immunotherapy, mainly owing to the high density of surrounding M2 macrophages, the exclusion of T cells, and the low expression of PD-L1 molecules. Thus, early identification of cold tumors through differences in the immunological environments of tumors would be promising to determine the optimal cancer treatment for each patient. Here, we developed a contrast agent targeting M2 macrophages to identify cold tumors by photoacoustic imaging. The polysaccharide-based contrast agent (denoted PMI) was synthesized by conjugating pullulan with mannose (M2 macrophage-targeting moiety) and IR-820 (near-infrared dye). In an aqueous solution, PMI formed a nanogel with a diameter of less than 100 nm via hydrophobic interactions involving IR-820 molecules. Owing to the IR-820 molecules, PMI nanogel absorbed near-infrared light, providing contrast for fluorescence and photoacoustic imaging. Moreover, M2 polarized RAW264.7 macrophage cells interacted with PMI nanogel but did not interact with IR-820 molecules and nanogels without mannose modification, indicating specific ligand–receptor interactions between mannose and CD206, a mannose receptor on M2 macrophages. Finally, PMI nanogel injected into colon26 tumor-bearing mice provided contrast for photoacoustic imaging of cold tumors. Therefore, PMI nanogel successfully targeted M2 macrophages to provide high contrast for photoacoustic imaging of cold tumors, demonstrating its potential as a platform for imaging and therapy of cold tumors.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"216 ","pages":"Article 114618"},"PeriodicalIF":4.9,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144686934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphologically tunable nickel metal-organic frameworks for highly selective and anti-fouling dopamine biosensing","authors":"Meng Nie ,&nbsp;Lijuan Chen ,&nbsp;Fan Chen ,&nbsp;Jinhao Wang ,&nbsp;Yu Bao ,&nbsp;Wei Wang ,&nbsp;Li Niu","doi":"10.1016/j.microc.2025.114616","DOIUrl":"10.1016/j.microc.2025.114616","url":null,"abstract":"<div><div>Metal-organic frameworks (MOFs) have emerged as promising candidates for electrochemical sensing due to their high specific surface area and tunable structural properties. However, achieving enhanced selectivity and stability remains a significant challenge. In this study, four distinct morphological variants of Ni-MOFs were successfully synthesized by modulating solvents and organic linkers, and subsequently evaluated for their potential in neurotransmitter dopamine (DA) biosensing. Structural characterization and electrochemical performances reveal that the spherical morphology of Ni-MOF-2 exhibits a significantly larger electrochemically active surface area and superior electrochemical oxidation activity towards DA compared to the other three bulkier Ni-MOFs. The limit of detection of Ni-MOF-2 for DA in simulated physiological fluids, specifically PBS, artificial sweat, artificial urine, and artificial cerebrospinal fluid (ACSF), were determined to be 36, 30, 58 and 43 nM, respectively, within the concentration range of 500 nM to 70 μM. FT-IR and Raman spectroscopy demonstrate that Ni-MOF-2 possessed excellent electrochemical stability and anti-fouling ability after continuous DA biosensing. Furthermore, Ni-MOF-2 exhibits outstanding long-term stability, excellent repeatability, and remarkable batch-to-batch consistency, demonstrating its potential as a reliable sensing material for practical electrochemical applications. These findings reveal the importance of morphological control in optimizing the performance of MOF-based electrochemical biosensors.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"216 ","pages":"Article 114616"},"PeriodicalIF":4.9,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}