Microchemical Journal最新文献

{"title":"From nanotechnology to AI: The next generation of CRISPR-based smart biosensors for infectious disease detection","authors":"Irkham ,&nbsp;Abdullahi Umar Ibrahim ,&nbsp;Chidi Wilson Nwekwo ,&nbsp;Pwadubashiyi Coston Pwavodi ,&nbsp;Salma Nur Zakiyyah ,&nbsp;Mehmet Ozsoz ,&nbsp;Yeni Wahyuni Hartati","doi":"10.1016/j.microc.2024.112577","DOIUrl":"10.1016/j.microc.2024.112577","url":null,"abstract":"<div><div>Infectious diseases caused by pathogens such as viruses, bacteria, fungi and parasites continues to be a burden to global healthcare sector. Early and accurate detection of these pathogens are crucial for timely and appropriate treatment. Several conventional techniques have been developed for the detection of these diseases; however, these techniques have several trade-offs which include low accuracy and sensitivity, laborious, time consuming, expensive, multiple steps, the use of hazardous chemicals and reagent and the requirement of sophisticated devices, lack of point of care testing as well challenges in automation. The integration of nanotechnology in biosensing technology offer several advantages which include rapid, sensitive and accurate detection of pathogenic disease. The increase demand for high specific, point-of-care (POC) and deployable biosensors has led to the advent of CRISPR-based biosensors which are initially discovered in microorganism as part of their adaptive immune system and subsequently programmed as gene editing tool. CRISPR/Cas systems have become a powerful tool for the detection of infectious diseases due to its specificity. The biosensing platform relies on the specificity of Cas system couple with NA amplification techniques which include recombinase polymerase amplification (RPA), polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP) etc. The integration of smart technology into biosensing techniques can enhance efficiency and data sharing. In this review, we will briefly discuss about the general characteristics and mechanisms of the CRISPR-Cas systems, overview conventional diagnostic approaches and biosensors, nanobiosensors, smart sensing technology, CRISPR-based biosensing strategies and prospect of smart CRISPR-based biosensors for POC testing.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112577"},"PeriodicalIF":4.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A fluorescent probe for selective detection of Pd2+ combined with fluorescent composite membranes and bioimaging","authors":"Haohu Li ,&nbsp;Wen Li ,&nbsp;Xinjie Yang ,&nbsp;Li Liu ,&nbsp;Haiyang Guo ,&nbsp;Xijuan Chai ,&nbsp;Linkun Xie ,&nbsp;Kaimeng Xu ,&nbsp;Guanben Du ,&nbsp;Lianpeng Zhang","doi":"10.1016/j.microc.2024.112370","DOIUrl":"10.1016/j.microc.2024.112370","url":null,"abstract":"<div><div>Palladium, as a transition metal of the platinum group elements, plays an indispensable role in chemical, pharmaceutical, and industrial production. However, its potential toxicity can pose risks to our health and the environment. In this work, a novel parallel alkyne-amine naphthalene ring fluorescent probe LNA based on Pd²⁺ coordination has been developed. The basic structure and detection mechanism of the probe have been confirmed by nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FTIR), and liquid chromatography-mass spectrometry (LC-MS). The probe LNA has a fluorescence-off response to the Pd²⁺ with high sensitivity (as fast as 60 s) and excellent selectivity (with a detection limit as low as 0.12 μM). Due to the outstanding fluorescence properties of LNA and the low cytotoxicity of its ligand complexes, it has been successfully applied to carboxymethyl cellulose-based fluorescent composite membranes as well as Pd²⁺ imaging of living cells.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112370"},"PeriodicalIF":4.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143095614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A simple and expeditious RP-HPLC method for the simultaneous determination of commercially available seven pesticides formulations","authors":"R. Singh,&nbsp;J. Dhalani","doi":"10.1016/j.microc.2024.112292","DOIUrl":"10.1016/j.microc.2024.112292","url":null,"abstract":"<div><div>For the purpose of simultaneously determination, a quick, easy, accurate and precise, high-performance liquid chromatographic analytical technique was validated for tolfenpyrad, pyraclostrobin, trifloxystrobin, azoxystrobin, fipronil, thiamethoxam, thiophanate-methyl, and azoxystrobin in their respective commercially accessible technical product compositions. Active components were separated chromatographically from additives and co-formulations using isocratic elution with 0.1 % orthophosphoric acid and acetonitrile in a 30:70 (v/v) at a flow rate of 1.0 mL per min on an Inertsil ODS-3 column (250 × 4.6 mm; 3.5 µm) with UV detection at 190 nm. With a 20-minute run time, the retention times for thiamethoxam, thiophanate-methyl, azoxystrobin, fipronil, pyraclostrobin, trifloxystrobin, and tolfenpyrad were found to be 2.96, 3.68, 5.98, 8.39, 11.31, 13.74, and 16.38 min, respectively. SANCO guidelines for precision, linearity, robustness, accuracy, LOD, specificity and selectivity were followed to approve the efficiency of this technique. Because all analyses were conducted using a single method and constant chromatographic settings, the suggested method offers several advantages, including rapid, sustainable, and eco-friendly analysis, as well as overall low costs. Developed method can be applied to water, food and different sources samples.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112292"},"PeriodicalIF":4.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143095618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A ratiometric fluorescent probe quickly responding for Pb2+ with the biggest red-shift stokes based on a new bimetallic MOF","authors":"Wei-Min Chen,&nbsp;Juan-Juan Shao,&nbsp;Fang-Ming Wang","doi":"10.1016/j.microc.2024.112333","DOIUrl":"10.1016/j.microc.2024.112333","url":null,"abstract":"<div><div>Here firstly report a novel 3D bimetallic MOF. It can be used as a fast ratiometric fluorescent probe and fast adsorbent for Pb²⁺ ions with its greatly fluorescence enhancement and a large red-shift stokes of 166 nm, which is the biggest shift of references. Moreover, it is also the first reported MOF material that can quantitatively analyze the lanthanide metal element Tb³⁺ among LMOFs. The limits of detection are calculated as 4.12 × 10⁻⁶ M and 2.94 × 10⁻⁵ M. ICP-OES testing and calculation showed that the maximum adsorption capacity of <strong>LMOF-1</strong> for Pb²⁺ was calculated to be 23.75 mg/g at five minutes.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112333"},"PeriodicalIF":4.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143096066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bimetallic zeolitic imidazolate frameworks-derived bar-shaped structure for electrochemical determination of H2O2","authors":"Minghong Fan ,&nbsp;Zhiyu Gong ,&nbsp;Jiayu Zheng ,&nbsp;Lu Zhou ,&nbsp;Beibei Qi ,&nbsp;Tianzuo Liu ,&nbsp;Zhengfei Yang","doi":"10.1016/j.microc.2024.112399","DOIUrl":"10.1016/j.microc.2024.112399","url":null,"abstract":"<div><div>Unique nanostructures of bimetallic materials derived from zeolitic imidazolate frameworks (ZIFs) are promising candidates for electrochemical sensor applications. In this study, different bimetallic bar-shaped ZIFs (B-ZIFs) were synthesized by replacing some cobalt ions with different metals ions (Ni²⁺, Ce²⁺, Cu²⁺, Mn²⁺, or Fe³⁺). Then their derivatives were prepared via carbonization, respectively. Upon conducting thorough material characterizations and assessment of the electrochemical properties, the most suitable candidate for electrode modification was identified as bar-shaped Co/Cu-based carbon materials (B-Co/Cu/Cs). This choice facilitated the construction of an innovative electrochemical sensor tailored for the determination of H₂O₂. The furnished B-Co/Cu/Cs-based sensor showed good linearity for H₂O₂ analysis within concentrations ranged from 0.5-4000 μM and 4000–11000 μM, with a detection limit of 0.44 μM. All these results suggested that the prepared material would be a competitive candidate for the non-enzymatic H₂O₂ sensing.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112399"},"PeriodicalIF":4.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143096313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in microfluidic platforms for rapid biomarker diagnostics of infectious diseases","authors":"Asma Vafadar ,&nbsp;Sara Takallu ,&nbsp;Shayan Khalili Alashti ,&nbsp;Sajad Rashidi ,&nbsp;Sonia Bahrani ,&nbsp;Amir Tajbakhsh ,&nbsp;Esmaeil Mirzaei ,&nbsp;Amir Savardashtaki","doi":"10.1016/j.microc.2024.112296","DOIUrl":"10.1016/j.microc.2024.112296","url":null,"abstract":"<div><div>Infectious diseases, caused by pathogenic microorganisms, such as bacteria, viruses, parasites, or fungi, continue to present a significant global threat. The need for accurate and sensitive detection techniques is underscored by the widespread impact of these diseases. Microfluidics, with its capacity to manipulate minute volumes of fluids within microscale channels, has facilitated the rapid development of sensitive assays for detecting disease biomarkers. This review focuses on recent advancements in microfluidics-based detection of infectious disease biomarkers, elucidating the critical role played by microfluidic techniques. The various configurations of microfluidic platforms discussed include Continuous-Flow Microfluidics, Droplet-Based Microfluidics, Digital Microfluidic, Paper-based Microfluidic, Centrifugal Microfluidics, Optofluidic, and Magnetofluidics. The article explores the advantages of microfluidics-based diagnostics, emphasizing attributes, such as reduced sample volume, swift analysis time, and enhanced sensitivity and specificity. Additionally, the challenges impeding the widespread adoption of microfluidics-based diagnostics in clinical settings are highlighted, providing insights into areas that require attention and innovation. In summary, this article underscores the potential of microfluidics technology to revolutionize healthcare by enabling early detection and personalized medicine in infectious disease diagnosis. The exploration of recent advancements and challenges aims to contribute to the ongoing dialogue surrounding the integration of microfluidics into clinical practice.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112296"},"PeriodicalIF":4.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143096493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micromolar-level glucose detection using a colorimetric sensor based on imprinted polymer-coated Fe3O4 nanoparticles","authors":"Ly Tan Nhiem ,&nbsp;Che Huynh Quang Quy ,&nbsp;Huynh Nguyen Anh Tuan ,&nbsp;Minh Huy Do ,&nbsp;Jin-Seo Noh ,&nbsp;Qui Thanh Hoai Ta","doi":"10.1016/j.microc.2024.112347","DOIUrl":"10.1016/j.microc.2024.112347","url":null,"abstract":"<div><div>The glucose concentration in the blood, commonly referred to as blood sugar level, typically ranges from 3.9 to 5.6 mmol/L. It is one of the most critical physiological parameters in the human body, requiring strict regulation to maintain proper bodily function. There is a strong demand for glucose sensors that are highly sensitive, user-friendly, and exhibit high selectivity towards glucose molecules, even in challenging environments. In this study, a colorimetric sensor based on imprinted polymer and Fe₃O₄ nanoparticles (NPs) was developed, enabling the detection of glucose at micromolar levels, observable by the naked eye. Fe₃O₄ NPs were prepared using a sol–gel method and served as cores for the deposition of polydopamine (PDA) via in situ polymerization. An imprinting process created glucose-imprinted PDA, resulting in molecular cavities on the PDA surface, with the polymeric layer approximately 25 nm thick. The surface of the Fe₃O₄ NPs facilitated the degradation reaction of H₂O₂ to produce hydroxyl radicals (•OH), which subsequently oxidized 3,3′,5,5′-Tetramethylbenzidine (TMB) to its oxidized form (TMB<strong>⁺</strong>), causing a color change from colorless to blue. The cavities played a crucial role in the sensing mechanism, connecting the catalytic surface of the Fe₃O₄ NPs to the surrounding environment. When glucose molecules occupied the cavities, the catalytic activity decreased, leading to a corresponding variation in color change depending on glucose concentration. Leveraging these advantages, the imprinted PDA was applied to detect glucose in aqueous solutions and human plasma with a limit of detection as low as 10 µM. The sensor exhibited high selectivity towards glucose over other common analytes in human blood, such as lysine, Ca²⁺, and K⁺, along with reproducibility, maintaining approximately 73 % of its sensing signal after six cycles of reuse. This study demonstrates the detection of glucose by the naked eye at micromolar levels. The detection procedure can be conducted outside of laboratory settings, presenting a highly promising approach for glucose detection in real-world applications, particularly in remote areas.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112347"},"PeriodicalIF":4.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143095616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant properties from Tagete filifolia essential oil with high estragole + anethole content by analysis of thermal stability and oxidation: chemical, volatile and sensory determinations","authors":"Gisela Kay Guerberoff Enemark ,&nbsp;Maria Elisa Mariani ,&nbsp;Carolina Florencia Cravero Ponso ,&nbsp;Nicolle Stefani Juncos ,&nbsp;Rubén Horacio Olmedo","doi":"10.1016/j.microc.2024.112393","DOIUrl":"10.1016/j.microc.2024.112393","url":null,"abstract":"<div><div><em>Tagete filifolia</em> essential oil (TEO) with a high content of estragole and anethole is thermally stable and protects lipids from oxidation, which allows it to be potentially used as an antioxidant. The objective of this research is to assess the TEO with high content (+95 %) of estragole and anethole (22.8 % and 77.0 % respectively) as a possible crop for industrial application, considering its potential antioxidant activity. The thermal stability of TEO (60 °C − 28 days) was determined by analysing its volatile chemical composition (SPME-GC–MS). Indirect antioxidant activity of TEO was performed with Free Radical Scavenging Activity (FRSA-DPPH) and Total Phenol Content (TPC). Direct measurement used an accelerated oxidation test at 60 °C for 14 days in concentrations of 0.02, 0.10 and 0.20 %w/w of EO in sunflower vegetable oil (chemical, volatile and sensory oxidation compounds were analysed). The results showed that anethole and estragole are thermally resistant, and together amount to 99.8 % after 28 days. Estragole decreased from 24.3 % to 21.3 %, while anethole increased from 75.1 % to 78.5 % between day 0 and day 28 of the trial. Indirect measurement revealed low FRSA (11.1 % of DPPH inhibition) and TPC (2.1 mg/g) activity. In the direct determination, protection against the control was observed (TOTOX Value Control &gt; TEO &gt; BHT0.02 %; 186, ≈ 150 and 134, respectively); However, the antioxidant activity did not show a direct correlation between the dose level used and the expected response. The results indicated that the essential oil could be applied as an antioxidant in a variety of products.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112393"},"PeriodicalIF":4.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143096284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel selective ionic liquid membrane-coupled microfluidic chip for heavy metal separation and analytical strategies","authors":"Yang Yuan ,&nbsp;Hui Jia ,&nbsp;Jibao Liu ,&nbsp;Fei Gao ,&nbsp;Xu Zhu ,&nbsp;Zihan An ,&nbsp;Jie Wang","doi":"10.1016/j.microc.2024.112321","DOIUrl":"10.1016/j.microc.2024.112321","url":null,"abstract":"<div><div>The development of new low-cost, highly selective and efficient methods for the separation and detection of heavy metal ions in aqueous environments is of great importance for water safety and biological health. In this study, a novel ion separation membrane was prepared for the separation of copper ions in cadmium ion detection solution. Compared with the commercial membrane, the ion mobility was increased by 64.02 %, and further separated by selective complexation of thiacalix[4]arene-p-tetrasulfonate (TCAS), and the separation factor for Cu/Cd reached 34.66. This allowed Cd to be detected preferentially, and reduced the interference of other ions in the detection. The joint electric field environment was regulated to separate the organics and reduce the interference to the detection. The sensing performance was enhanced by modifying the screen-printed electrodes. The proposed sensing platform allows for simultaneous pre-processing and detection and has a detection limit of 0.19 μg-L⁻¹ (S/N = 3) for Cd²⁺.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112321"},"PeriodicalIF":4.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143096285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-assemblies of porphyrin tubular cage/polylysine based 1D nanoribbons with controllable handedness for enantioselective detection of theanine and mechanism revealing","authors":"Yuying Jiang,&nbsp;Jingxiu Bi,&nbsp;Rui Gao,&nbsp;Pingxiang Liu,&nbsp;Chao Zhang,&nbsp;Tong Zhao,&nbsp;Xuexia Yuan,&nbsp;Yutao Wang","doi":"10.1016/j.microc.2024.112365","DOIUrl":"10.1016/j.microc.2024.112365","url":null,"abstract":"<div><div>1D nanoribbons with supramolecular controllable handedness are constructed by the co-assemblies of porphyrin tubular cage (PTC) with polylysine (PL) upon multiple intermolecular forces at the air/water interface. Depending on the outstanding stimuli-responsive performance of aggregating hierarchical nanoribbons, the enantiomers of theanine are clearly discriminated through the relative fluorescence intensity of co-assembling system. It is revealed that the enantioselective mechanism originate from the quite disparate intermolecular interactions between co-assemblies and theanine isomers induced selective adsorption by means of experimental characterization combined with molecular simulations. This study not only helps to further understand the multiple interaction within co-assembling system, but also provides a novel strategy for enantioselective sensing platform with controllable handedness.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112365"},"PeriodicalIF":4.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143096311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}