Research progress on quantitative detection of foodborne pathogenic bacteria based on lateral flow assays

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal Pub Date : 2025-09-09 DOI:10.1016/j.microc.2025.115237

Yan Pan , Shijian Liu , Wenjin Ma , Xiaoqiong Li , Xiaoqi Tao

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Abstract

The lateral flow assay (LFA) provides critical technical support for rapid, low-cost, on-site quantitative detection of foodborne pathogenic bacteria. This article reviews common strategies for enriching pathogenic bacteria in food matrices and explains the fundamental principles of detecting foodborne pathogenic bacteria using LFA. Furthermore, the article summarizes the quantitative LFA platform for detecting foodborne pathogenic bacteria, such as colorimetry, fluorescence, surface-enhanced Raman spectroscopy (SERS), and photothermal effect methods (PTE), along with their recent advancements in bacterial quantification. Finally, the future prospects and challenges of LFA for foodborne pathogenic bacteria detection are discussed. The construction of a standardized production system, the development of low-cost and high-stability materials, the optimization of regulatory pathways, and the innovation of portable intelligent reading devices are expected to promote the large-scale application of LFA in on-site detection and point-of-care diagnosis of foodborne pathogenic bacteria.

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基于横向流动法的食源性致病菌定量检测研究进展

横向流动试验（LFA）为快速、低成本、现场定量检测食源性致病菌提供了关键的技术支持。本文综述了在食品基质中富集致病菌的常用方法，并阐述了LFA检测食源性致病菌的基本原理。此外，本文总结了用于检测食源性致病菌的定量LFA平台，如比色法、荧光法、表面增强拉曼光谱法（SERS）和光热效应法（PTE），以及它们在细菌定量方面的最新进展。最后，讨论了LFA在食源性致病菌检测中的应用前景和面临的挑战。标准化生产体系的构建、低成本高稳定性材料的开发、调控途径的优化以及便携式智能阅读设备的创新，有望推动LFA在食源性致病菌现场检测和护理点诊断中的大规模应用。

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来源期刊

Microchemical Journal 化学-分析化学

CiteScore

8.70

自引率

8.30%

发文量

1131

审稿时长

1.9 months

期刊介绍： The Microchemical Journal is a peer reviewed journal devoted to all aspects and phases of analytical chemistry and chemical analysis. The Microchemical Journal publishes articles which are at the forefront of modern analytical chemistry and cover innovations in the techniques to the finest possible limits. This includes fundamental aspects, instrumentation, new developments, innovative and novel methods and applications including environmental and clinical field. Traditional classical analytical methods such as spectrophotometry and titrimetry as well as established instrumentation methods such as flame and graphite furnace atomic absorption spectrometry, gas chromatography, and modified glassy or carbon electrode electrochemical methods will be considered, provided they show significant improvements and novelty compared to the established methods.