Microchemical Journal最新文献

{"title":"Recent advances in bacterial lysis techniques for environmental monitoring: A review","authors":"Jeongeun Lee ,&nbsp;Beelee Chua ,&nbsp;Ahjeong Son","doi":"10.1016/j.microc.2024.111865","DOIUrl":"10.1016/j.microc.2024.111865","url":null,"abstract":"<div><div>Bacterial lysis is a critical step in bioassays and serves as a pretreatment to extract genomic material before various genomic bioassays. These assays are used for microbial profiling, identification, or quantification. In the realm of environmental monitoring, the effective management of microbial environments, such as soil with nitrogen-fixing bacteria or lakes and rivers with algal blooms, is crucial. To achieve successful microbial detection and environmental monitoring, we explored various existing bacterial lysis techniques. Initially, we discuss the general techniques for chemical and physical bacterial lysis that have been optimized and integrated into commercial DNA extraction kits, the current gold standard for bacterial lysis. Next, we introduce recent advancements in lysis technologies, particularly in the context of rapid, portable, and on-site implementation, where traditional DNA extraction kits may be time consuming or impractical. Finally, we provide a concise overview of developments in portable devices and systems designed for bacterial cell lysis. This comprehensive review of the bacterial lysis techniques research will improve analytical methods in environmental microbiology and biotechnology.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111865"},"PeriodicalIF":4.9,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in the application of nanomaterials for separation and enrichments of small molecule active ingredients of traditional Chinese medicine","authors":"Yi-zhen Sun,&nbsp;Ying Zhou,&nbsp;Xu-zhao Li,&nbsp;Shuai-nan Zhang","doi":"10.1016/j.microc.2024.111827","DOIUrl":"10.1016/j.microc.2024.111827","url":null,"abstract":"<div><div>In this review, we focused on the nanomaterials and applications used to separate and enrich the small molecule active components of traditional Chinese medicine (TCM) in the past 5 years (2018–2023), including magnetic molecularly imprinted polymers (MMIPs), magnetic surface functionalized dummy template molecularly imprinted polymers (magnetic surface functionalized DMIPs), borate functionalized magnetic solids (BFMS), metal–organic framework materials (MOFs) and molecularly imprinted mesoporous silica (MIP-MS). This review provides a reference for further innovation and development of separation and enrichment technology of pharmacodynamic substances in TCM. Among them, MMIPs have been applied more in the active ingredients of small molecules in TCM, mainly for flavonoids, terpenoids, alkaloids and organic acids, and this technology is more advanced. Magnetic surface functionalized DMIPs have high specificity and selectivity for target analytes, which can shorten the separation and enrichment time and increase the yield. The use of this manomaterial for the separation and enrichment of some natural active ingredients can effectively make up for the shortcomings of traditional MIPs. In addition, studies in the past 5 years have also shown some progress in the separation and enrichment of small molecule components by BFMS, MOFs and MIP-MS. For example, BFMS can be used to separate glycosides, MOFs to separate flavonoids, and MIP-MS to separate organic acids. However, the application of these materials for small molecules of TCM is still insufficient, including fewer application examples in the field of chromatography, and some of the materials have never even been utilized. Meanwhile, most of them have only been initially applied to solid-phase extraction in the pre-treatment of chromatography. Therefore, we believe that we can try to apply these nanomaterials more to the separation and enrichment of the active ingredients of small molecules in TCM afterwards, thus advancing the development of chromatography.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111827"},"PeriodicalIF":4.9,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanomaterials as signal amplifiers in CRISPR/Cas biosensors: A path toward multiplex point-of-care diagnostics","authors":"Fareeha Arshad,&nbsp;Bong Jing Yee,&nbsp;Koo Pey Ting,&nbsp;Arifah Arina Syairah Janudin,&nbsp;Muhammad Nadzre Adzremeen bin Amir,&nbsp;Minhaz Uddin Ahmed","doi":"10.1016/j.microc.2024.111826","DOIUrl":"10.1016/j.microc.2024.111826","url":null,"abstract":"<div><div>With the recent shift in perspective in compacting diagnostic laboratories to recreate miniaturised and portable diagnostic tools that can perform rapid, on-site detection, novel nanomaterials have been explored for their application in developing unique point-of-care systems. Upon integration with CRISPR/Cas, it can perform large-scale screening and potentially be applied even under remote conditions, especially in sudden outbreaks and pandemics. Despite the high <em>trans</em>-cleavage activity of the CRISPR/Cas system to give a rapid fluorometric response, their application towards detecting target analytes from complex samples suffers from limited stability and sensitivity. On the contrary, nanomaterials demonstrate robust activity and versatile properties, including rapid optical and electrocatalytic activity, which places them in an ideal position to be applied as signal amplifier systems along with CRISPR/Cas. With the integration of <em>trans</em>-cleavage activity of CRISPR/Cas and nanomaterials, it is possible to develop the next generation of diagnostics that can detect various kinds of biomarkers using different optical and electrochemical transduction methods, which play a crucial role in personalised medicine and precision agriculture. Through this review, we aim to explore the recent advances in the field of CRISPR/Cas – nanomaterial-based biosensors that offer the promising potential to be applied in the highly sensitive and selective detection of multiple target analytes. The potential of applying such biosensors towards developing multiplex biosensors will also be explored, followed by the outlook of this promising diagnostic technology.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111826"},"PeriodicalIF":4.9,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging trends: Smartphone-assisted aptasensors enabling detection of pathogen and chemical contamination","authors":"Priyanka Garg ,&nbsp;Ritika Gupta ,&nbsp;Nitesh Priyadarshi ,&nbsp;Poonam Sagar ,&nbsp;Vishakha Bisht ,&nbsp;Naveen K. Navani ,&nbsp;Nitin Kumar Singhal","doi":"10.1016/j.microc.2024.111736","DOIUrl":"10.1016/j.microc.2024.111736","url":null,"abstract":"<div><div>The timely and rapid detection of pathogens and environmental contaminants is critical in safeguarding human health and preventing crises. Pathogens, antibiotic residues, pesticides, and heavy metals pose significant threats to human lives and the economy due to their rapid spread. Developing effective methods for detecting and preventing contaminant spread remains a major challenge. Researchers have been exploring innovative sensing approaches to enable easy, portable, and cost-effective detection using smartphones as versatile sensing tools in recent years. Integrating smartphones with detection assays allows for convenient result acquisition through a simple photograph. Aptamers, with their high affinity and selectivity, have gained substantial interest in biosensing, and they are increasingly employed in the design of novel detection assays based on optical or electrochemical principles. Additionally, smartphone applications can be developed to process images and analyze results based on optical or electrochemical signals, accurately determining pathogen loads. Apart from aptamers, smartphone-based detection platforms also utilize other sensing elements, such as antibodies, carbohydrates, and proteins. Coupling smartphones with detection assays simplifies the detection procedure, making it portable and highly sensitive. This review focuses on the latest technological advancements in smartphone-based devices for biosensing applications, highlighting their potential for rapid pathogen and contaminant detection.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111736"},"PeriodicalIF":4.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review of state-of-the-art biosensors for detection of allergenic substance lysozyme: A structured recent review and update","authors":"Alireza Ebrahimi ,&nbsp;Reza Abedi-Firoozjah ,&nbsp;Ali Ehsani ,&nbsp;Hamed Hamishehkar ,&nbsp;Milad Tavassoli","doi":"10.1016/j.microc.2024.111805","DOIUrl":"10.1016/j.microc.2024.111805","url":null,"abstract":"<div><div>Lysozyme (LZM) is a major allergenic protein found in different foods, such as chicken eggs, primarily in the egg white. It can cause adverse reactions in sensitive individuals, leading to symptoms ranging from mild hives to life-threatening anaphylactic shock. Therefore, it’s crucial for those with allergies to avoid any contact with LZM and foods containing it. Reliable analytical methods are essential to ensure consumer safety and improve food production through hazard analysis and good manufacturing practices. The following review article provides an overview of recent progress in biosensors used for the detection of LZM. This article developed a comprehensive review of the main applications of LZM detection, emphasizing the advantages and disadvantages of common analytical techniques such as conventional methods (DNA-based methods and ELISA/Immunochromatography assay/Immunoassay/Mass spectrometry) and modern methods (biosensors such as electrochemical, optical, molecularly imprinted polymers and magnetoelastic sensing). Subsequently, it delves into the developments in biosensors pertaining to the detection mechanisms, including the utilization of nanomaterials as signal probes and carriers for loading signal probes, while also addressing their sensitivity and performance evaluation. Lastly, it discusses the challenges and potential opportunities in the advancement of biosensors for LZM detection.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111805"},"PeriodicalIF":4.9,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developments and trends in mycotoxin analysis: A review with bibliometric research","authors":"Chuang Wang ,&nbsp;Ying Huang ,&nbsp;YunYun Wang ,&nbsp;Jing Zhang ,&nbsp;MengYue Guo ,&nbsp;ChangYong Chen ,&nbsp;HuiRu Zhang ,&nbsp;BinYang Yue ,&nbsp;DanDan Kong ,&nbsp;JiaoYang Luo ,&nbsp;MeiHua Yang","doi":"10.1016/j.microc.2024.111774","DOIUrl":"10.1016/j.microc.2024.111774","url":null,"abstract":"<div><div>Food safety is crucial for human health, and mycotoxins, produced by fungi, can contaminate food, posing a serious health threat. Understanding mycotoxin production, toxicity mechanisms, and accurate detection is essential for food safety. While many studies have explored these aspects, there is still room for improvement in detection methods for mycotoxins. Taking mycotoxins as an entry point, this paper systematically collected and analyzed mycotoxin-related literatures from mainstream databases by combining bibliometric analysis, revealing the development trend and research focuses in this field, confirming mycotoxin detection methods as the core of this review, and further discussing chromatography-based, spectrometry-based, immunoassay-based, biosensor-based, and other. Research advances and limitations of mycotoxin detection methods, such as high sensitivity versus cumbersome processing for chromatography, low cost for spectroscopy, and high specificity for immunoassays, were further discussed. Emerging technologies like immunoassays and biosensors show promise for rapid and portable detection. Challenges in sample preparation and matrix effects are also discussed. Meanwhile, by analyzing the development trend of mycotoxin detection methods, it is predicted that the future research focus will be mainly on the two aspects of highly sensitive and accurate detection and rapid and accurate on-site detection. This review aims to provide insights for the field’s development, predict future trends in mycotoxin analysis, and enhance food safety and public health protection.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111774"},"PeriodicalIF":4.9,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic profiling of plant and other natural products: Platforms, applications and quality evaluation","authors":"Adeyemi O. Adeeyo ,&nbsp;Isaac O. Amao ,&nbsp;Yvan A.T. Ngandjui ,&nbsp;Mercy A. Alabi ,&nbsp;Titus A.M. Msagati","doi":"10.1016/j.microc.2024.111779","DOIUrl":"10.1016/j.microc.2024.111779","url":null,"abstract":"<div><div>This review unveils the possibility of metabolomics in natural product study and documents available techniques, strategies, and platforms for metabolite profiling. Metabolomics process flow including experimental designs, sample preparations, data acquisition, mining, and results interpretation are presented. The leveraging of metabolomics for maximizing natural product development and sustainable plant use was explored. The extensive review and presentation of details in metabolomics, lead medicine discoveries and frameworks for quality control, fill important research gaps and expand frontiers of knowledge within the field of study.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111779"},"PeriodicalIF":4.9,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An advanced early warning technology for coral bleaching crisis based on the electrochemical biosensing of coral-symbiotic zooxanthellae eDNA","authors":"Chaoxin Zhang ,&nbsp;Hongjie Liu ,&nbsp;Shaopeng Wang ,&nbsp;Man Zhang ,&nbsp;Jiayuan Liang ,&nbsp;Hao Fu ,&nbsp;Yingmei Wei ,&nbsp;Kefu Yu ,&nbsp;Liwei Wang","doi":"10.1016/j.microc.2024.111775","DOIUrl":"10.1016/j.microc.2024.111775","url":null,"abstract":"<div><div>The symbiotic <em>zooxanthellae</em> in corals are extremely sensitive to the marine environment, and corals bleach rapidly when the symbiotic <em>zooxanthellae</em> are detached from their coral hosts. Currently, ecological monitoring techniques face many problems in coral health assessment, especially coral reef destruction during sampling. In this paper, an electrochemical eDNA biosensor based on ECM/MoS₂ nanocomposites constructed with specific DNA probes was developed to detect the environmental DNA of free <em>zooxanthellae</em> at a low limit of detection (LOD = 0.223 pM) and limit of quantification (LOQ = 0.743 pM) under the experimentally optimized conditions, demonstrating excellent specificity and steadiness. Over and above, the electrochemical response of the biosensor to <em>zooxanthellae</em> DNA was significantly higher than that of other interferent targets, proving its high selectivity. On this basis, the dependability and precision of the biosensor were further verified by comparing the biosensor with ddPCR (<em>P</em> &gt; 0.05, RSD &lt; 5 %). Hence, this electrochemical biosensor can dynamically monitor community changes in <em>zooxanthellae</em> without destroying coral reefs and may serve as a non-invasive method for early warning of coral reef crises. The technique can also be upgraded with portable capabilities in future studies to provide greater convenience in the field analysis of coral bleaching.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111775"},"PeriodicalIF":4.9,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142357865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-mode colorimetric–fluorometric immunoassay for foodborne parasitic infection","authors":"Yao Yu ,&nbsp;Xiaolei Liu ,&nbsp;Ning Xu ,&nbsp;Lin Li ,&nbsp;Yaming Yang ,&nbsp;Mingyuan Liu ,&nbsp;Lianjing Zhao ,&nbsp;Xue Bai","doi":"10.1016/j.microc.2024.111778","DOIUrl":"10.1016/j.microc.2024.111778","url":null,"abstract":"<div><div>Immunoassays are the primary method used for detection of foodborne parasitic infection. The nanomaterials used in these assays often present challenges such as complex preparation requirements and low stability during long-term storage and transport. Herein, based on cost-effective K₃[Fe(CN)₆]-mediated in-situ generated CuFe Prussian blue analogues (CuFe-PBA) and quinoxaline derivatives with high fluorescence, a dual-mode immunoassay was proposed for foodborne parasitic infection. This platform is relied on the restoration of K₃[Fe(CN)₆] by ascorbic acid (AA). In addition to this process, K₄[Fe(CN)₆]-triggered the generation of CuFe-PBA, and the enhanced fluorogenic reaction of dehydrogenated AA and <em>o</em>-phenylenediamine (OPD) occur sequentially. Without complex probes preparation, the assay enabled the rapid on-site detection of ALP and foodborne parasitic infection through a colorimetric mode accessible via smartphone and offered enhanced sensitivity through a fluorescent mode. The method was used for the detection of ALP, achieving detection limits of 0.38 U/L and 0.03 U/L for colorimetric and fluorescence modes, respectively. Subsequently, the dual-mode immunoassay was applied to <em>Trichinella spiralis</em> infection. The sensitivity and specificity of both the colorimetric and fluorescence modes were 100 % in 950 pig serum samples. This immunosensor provides a universal analysis tool for the accurate, cost-effective and highly sensitive detection of foodborne parasitic infection.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111778"},"PeriodicalIF":4.9,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142357866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combination aquaphotomics study and LF-NMR to monitor the drying process of honey pills","authors":"Mengyin Tian ,&nbsp;Xiaobo Ma ,&nbsp;Lian Li ,&nbsp;Aoli Wu ,&nbsp;Xue Gao ,&nbsp;Zhaoqing Meng ,&nbsp;Guiyun Cao ,&nbsp;Yi Luo ,&nbsp;Lei Nie ,&nbsp;Hengchang Zang","doi":"10.1016/j.microc.2024.111772","DOIUrl":"10.1016/j.microc.2024.111772","url":null,"abstract":"<div><div>As one of the factors affecting the quality of honey pills (HP), there are few researches on its quality control and solutions. But water can directly affect the appearance, stability and potency of the HP. In this study, near-infrared spectroscopy was used to monitor the moisture content of HP with different drying conditions, and multiple linear regression analysis was carried out to establish the moisture content prediction model, and the R² of the model prediction is 0.9532. The visualization of HP drying process were carried out by aquaphotomics method characterizes the status of the water molecular network at different drying stages. At the same time, low-field nuclear magnetic resonance (LF-NMR) information was carried out to analyze the water distribution in the drying process, and further verified the water solvent shell action in the water balance process of aquaphotomics analysis. The surface water of the pill disperses to the air, and the internal water diffuses outwards. We adopted the techniques such as rheology to comprehensively characterize the state of HP, and to evaluate the quality of them by using the dissolution test. The quality state of the pills was analyzed by the aquaphotomics analysis of water species. The above research system provided the scientific basis for revealing the drying process of HP from the viewpoint of aquaphotomics, as well as showed potential for expansion and application to other Chinese traditional medicine products.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111772"},"PeriodicalIF":4.9,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142357867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}