Microchemical Journal最新文献_第5页

Fabricating a sensitive electrochemical aptasensor based on HOF/MXene nanocomposites for monitoring trace ampicillin

IF 4.9 2区化学

Microchemical Journal Pub Date : 2025-04-11 DOI: 10.1016/j.microc.2025.113625

Shan Zhang , Jie Xi , Xuan Yu , Hongming He , Xinyue Yang , Yatian Zhao , Xinyi Wang , Mingkai Xu , Shuting Du , Cheng-Peng Li , Fuxing Sun , Yongchang Ling

引用次数: 0

Advances in electrochemical biosensors for the diagnosis of tuberculosis: Perspectives for control and eradication of the disease

IF 4.9 2区化学

Microchemical Journal Pub Date : 2025-04-11 DOI: 10.1016/j.microc.2025.113622

Amando Oliveira Matias , Geanderson Emílio de Oliveira , Emanuel Airton de Oliveira Farias , Joseph Wang , Carla Eiras , Anderson Nogueira Mendes

{"title":"Advances in electrochemical biosensors for the diagnosis of tuberculosis: Perspectives for control and eradication of the disease","authors":"Amando Oliveira Matias , Geanderson Emílio de Oliveira , Emanuel Airton de Oliveira Farias , Joseph Wang , Carla Eiras , Anderson Nogueira Mendes","doi":"10.1016/j.microc.2025.113622","DOIUrl":"10.1016/j.microc.2025.113622","url":null,"abstract":"<div><div>Tuberculosis is a severe contagious disease caused by <em>Mycobacterium tuberculosis</em> (Mtb), whose epidemic has persisted since its discovery in 1882. Despite global efforts to combat tuberculosis, which include financial support for prevention, diagnosis, and treatment, relapses of latent patients and an increase in cases of multidrug-resistant tuberculosis contribute to the non-eradication of the disease. Therefore, overcoming the difficulties in developing assays for point-of-care diagnosis has become a necessary priority in the fight against this pathogen. In this sense, substantial research efforts have been devoted to advancing electrochemical biosensors for detecting Mycobacterium tuberculosis and diagnosing Tuberculosis in centralized and decentralized settings. Thus, this work sought to address the main <em>Mycobacterium tuberculosis</em> antigens that have been screening targets during the development of immunosensors and electrochemical genosensors for diagnosing tuberculosis, analyzing the manufacturing strategies, validation, and analytical characteristics of these devices. Here, an in-depth discussion is provided in pursuit of understanding the main advances and challenges in overcoming limitations in tuberculosis diagnosis, including cases of latent tuberculosis or co-infected patients. Finally, we present a technological prospection, where the main patents filed, and technological products related to tuberculosis diagnosis were evaluated. Therefore, this study’s scientific and technological survey revealed that biosensors aimed at diagnosing tuberculosis still need to overcome some difficulties for practical and safe use. Furthermore, the new insights described in this work reveal valuable information about monitoring biointeractions that can support the development of new methods for diagnosis of tuberculosis.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"213 ","pages":"Article 113622"},"PeriodicalIF":4.9,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Au@Cu2O@SDS composite nanorods for dual-mode sensing of histidine

IF 4.9 2区化学

Microchemical Journal Pub Date : 2025-04-11 DOI: 10.1016/j.microc.2025.113621

Chuang Wen, Zhongwei Lin, Yilin Wang

{"title":"Au@Cu2O@SDS composite nanorods for dual-mode sensing of histidine","authors":"Chuang Wen, Zhongwei Lin, Yilin Wang","doi":"10.1016/j.microc.2025.113621","DOIUrl":"10.1016/j.microc.2025.113621","url":null,"abstract":"<div><div>The content of histidine (His) in urine is one of the important reference indicators for the diagnosis of certain diseases. The research on using nanomaterials as substitutes for organic fluorescent probes and natural enzymes is receiving increasing attention. In this study, Au@Cu<sub>2</sub>O composite nanorods (CNRs) coated with sodium dodecyl sulfate (SDS) (Au@Cu<sub>2</sub>O@SDS CNRs) were prepared. It was found that the obtained Au@Cu<sub>2</sub>O@SDS CNRs emits weak fluorescence at 372 nm when excited at 280 nm, and the presence of His leads to an enhanced fluorescence. On the other hand, the Au@Cu<sub>2</sub>O@SDS CNRs exhibits oxidase-like activity, which can catalyze the oxidation of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) without the need of H<sub>2</sub>O<sub>2</sub> to form blue-colored oxidized TMB (TMBox). However, the addition of His results in a decrease in absorbance at 652 nm. Thus, a fluorescence and colorimetric dual-mode sensing system responsive to His was successfully constructed. Under the optimum conditions, the increase in fluorescence (Δ<em>F</em>) and decrease in absorbance (Δ<em>A</em>) of the system are closely related to His concentration ranging from 0.5-60 μM and 0.3–25 μM with the detection limit of 0.22 and 0.26 μM, respectively. More importantly, the dual-mode sensing system was utilized for the detection of His in human urine samples with satisfactory recovery (90.1 % ∼109.4 %), confirming its good potential for application.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"213 ","pages":"Article 113621"},"PeriodicalIF":4.9,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lanzhou lily as nutraceuticals: Identification of active metabolites via the UHPLC-Q-exactive orbitrap mass spectrometer

IF 4.9 2区化学

Microchemical Journal Pub Date : 2025-04-11 DOI: 10.1016/j.microc.2025.113626

Zi-Yi An , Jun-Min Jin , Zi-Jian Tan , Yong-Fei Wang , Ting Wang , Zi-Kai Dong , Jian-Jun Chen , You-Cai Xiong , Wei-Lin Jin

{"title":"Lanzhou lily as nutraceuticals: Identification of active metabolites via the UHPLC-Q-exactive orbitrap mass spectrometer","authors":"Zi-Yi An , Jun-Min Jin , Zi-Jian Tan , Yong-Fei Wang , Ting Wang , Zi-Kai Dong , Jian-Jun Chen , You-Cai Xiong , Wei-Lin Jin","doi":"10.1016/j.microc.2025.113626","DOIUrl":"10.1016/j.microc.2025.113626","url":null,"abstract":"<div><div>Lanzhou lily/Baihe (<em>Lilium davidii</em> var. <em>unicolor</em>) is recognized as a traditional medicinal plant in China and is consumed as an edible bulbous vegetable, known for its numerous health benefits. However, comprehensive studies on its chemical composition remain limited. Therefore, in this study, we employed ultra-high-performance liquid chromatography-Q Exactive Orbitrap mass spectrometry (UHPLC-Q Exactive Orbitrap-MS) to systematically analyze and identify the chemical constituents of Lanzhou lily.</div><div>A total of 210 compounds were identified from Lanzhou lily, including 43 glycosides and carbohydrates, 39 phenylpropanoids, 30 terpenes, 21 flavonoids, 19 amino acids and derivatives, 11 fatty acyl,9 phenols, 7 organic acids and their derivatives, 5 carboxylic acids and their derivatives,4 pyridines and derivatives, 5 alkaloids,3 steroids compounds and other phytochemicals, of which 184 were reported for the first time in Lanzhou lily. These active ingredients support the nutritional benefits and medicinal properties of Lanzhou lily. This study provides insight into the chemical properties of Lanzhou lily and may help further research to identify the factors that lead to the health benefits of this plant.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"213 ","pages":"Article 113626"},"PeriodicalIF":4.9,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single probe-based sensor array integrated with 3D-printed smartphone platform for the identification of guanine nucleotides, anthrax biomarkers and their derivatives 基于单探针的传感器阵列与 3D 打印智能手机平台集成，用于鉴定鸟嘌呤核苷酸、炭疽生物标记物及其衍生物

IF 4.9 2区化学

Microchemical Journal Pub Date : 2025-04-11 DOI: 10.1016/j.microc.2025.113629

Yan-Xin Guo, Lei Wang, Fei Gao, Xin-Yue Chen

{"title":"Single probe-based sensor array integrated with 3D-printed smartphone platform for the identification of guanine nucleotides, anthrax biomarkers and their derivatives","authors":"Yan-Xin Guo, Lei Wang, Fei Gao, Xin-Yue Chen","doi":"10.1016/j.microc.2025.113629","DOIUrl":"10.1016/j.microc.2025.113629","url":null,"abstract":"<div><div>Sensor array has gained significant attention in the sensing field owing to its unique properties, which allow it to simultaneously detect and distinguish multiple targets. The sensor array mainly relies on a variety of sensing elements to build multiple detection channels so as to function. Among these, the design of multiple sensing elements is relatively complicated. In this paper, a novel strategy was proposed to construct sensor array, which could generate multiple signals through a single probe without the need to build multiple sensing elements, thereby enabling the fast and efficient identification of various analytes. A fluorescence sensor based on ZIF-8@GSH-AuNCs-Tb was constructed by encapsulating glutathione-capped gold nanoclusters (GSH-AuNCs) and terbium ions (Tb<sup>3+</sup>) within zeolitic imidazolate framework-8 (ZIF-8) for the simultaneous discrimination of guanine nucleotides, anthrax biomarkers and their derivatives. Due to its special properties, Tb<sup>3+</sup> could produce three characteristic emission wavelengths of 492, 547, and 585 nm when excited at 256 nm. Surprisingly, each output signal could be combined with the emission signal of GSH-AuNCs to form ratio fluorescence for the quantification of targets, thus obtaining a three-channel sensor array. A large amount of data was collected to complete the calculation of principal component analysis (PCA), hierarchical cluster analysis (HCA), and other statistical methods, so as to realize the identification of multiple targets. Besides, with the help of 3D printing technology, a portable smartphone platform integrated with the testing paper was developed, showing excellent sensing and recognition ability in serum sample detection.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"213 ","pages":"Article 113629"},"PeriodicalIF":4.9,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-isotope calibration strategy for trace elements determination in whole blood by inductively-coupled plasma mass spectrometry

IF 4.9 2区化学

Microchemical Journal Pub Date : 2025-04-11 DOI: 10.1016/j.microc.2025.113628

Poliana Borges de Oliveira , Maiara Krause , Luiza Valli Vieira , Luana Santos Moreira , Alex Virgilio , Geisamanda Pedrini Brandão , Maria Tereza Weitzel Dias Carneiro

{"title":"Multi-isotope calibration strategy for trace elements determination in whole blood by inductively-coupled plasma mass spectrometry","authors":"Poliana Borges de Oliveira , Maiara Krause , Luiza Valli Vieira , Luana Santos Moreira , Alex Virgilio , Geisamanda Pedrini Brandão , Maria Tereza Weitzel Dias Carneiro","doi":"10.1016/j.microc.2025.113628","DOIUrl":"10.1016/j.microc.2025.113628","url":null,"abstract":"<div><div>This study proposes the multi-isotopic calibration strategy (MICal) for determining Cd, Cr, Hg, Mo, Ni, Pb, Se, Sn, and Zn in whole blood samples by inductively coupled plasma mass spectrometry. Isotopes were monitored in only two solutions: A: (5 % (v/v) sample + 95 % (v/v) standard) and B (5 % (v/v) sample + 95 % (v/v) blank). Different standard concentrations were tested within MICal to improve linearity (R<sup>2</sup> > 0.95), enhancing model robustness. Calibration curves were built by plotting signals from solutions A and B on the x and y axes, respectively. Analyte concentrations were determined from the slope and standard concentrations in solution A. Slopes near 0.5 were achieved, and ANOVA and Tukey’s test assessed significant differences. MICal has the unique advantage of identifying spectral interferences by observing outlier values in the calibration curve, which were detected for Cr, Ni, and Se. MICal exhibited superior performance compared to external calibration (EC) and standard addition (SA) for Cr, Hg, Mo, Sn, and Zn, achieving improved sensitivity and accuracy. Additionally, MICal uniquely enables the identification of spectral interferences by detecting outliers in the calibration curve, as observed for Cr, Ni, and Se. The method demonstrated low limits of quantification (LOQ) and agreement values within an acceptable range, comparable to the SA strategy, which is typically used for complex matrices. MICal is a robust and efficient calibration approach for whole blood analysis, offering advantages such as reduced analysis time, lower cost, simplicity, and interference detection.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"213 ","pages":"Article 113628"},"PeriodicalIF":4.9,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strategical design of ZnWO4@g-C3N4 nanocomposite: An affordable and efficient photoelectrochemical sensor for the detection of Candida albicans

IF 4.9 2区化学

Microchemical Journal Pub Date : 2025-04-11 DOI: 10.1016/j.microc.2025.113619

Babu Shobana , Kathirvel Renugadevi , Periakaruppan Prakash

{"title":"Strategical design of ZnWO4@g-C3N4 nanocomposite: An affordable and efficient photoelectrochemical sensor for the detection of Candida albicans","authors":"Babu Shobana , Kathirvel Renugadevi , Periakaruppan Prakash","doi":"10.1016/j.microc.2025.113619","DOIUrl":"10.1016/j.microc.2025.113619","url":null,"abstract":"<div><div>Detecting fungi is crucial for addressing serious health risks, as early identification facilitates prompt treatment, reducing complications and enhancing patient diagnosis. Accurate detection of specific fungi is vital for targeted therapy, particularly in the case of <em>Candida species</em>, a prevalent pathogen requiring precise identification for effective management of conditions like invasive candidiasis in hospitalized patients. Conventional methods for detecting <em>Candida albicans (C. albicans)</em> face challenges such as extended processing times, limited sensitivity, and the potential for false results. Polymerase Chain Reaction (PCR), encounters issues like contamination and variations in DNA concentration. We introduce a highly sensitive photoelectrochemical sensor (PEC) for detecting <em>C. albicans</em> at low levels, using a zinc tungstate −doped graphitic carbon nitride nanocomposite (ZnWO<sub>4</sub>@g-C<sub>3</sub>N<sub>4</sub> nanocomposite). This nanocomposite efficiently captures and transduces signals associated with the fungus, enabling rapid and accurate detection. The nanocomposite, synthesized through co-precipitation, underwent thorough characterization. Notably, the research achieved an unprecedented small detectable limit (LOD: 1 CFU mL<sup>−1</sup>, LOQ: 4 CFU mL<sup>−1</sup>) with detection range from 1 to 15 CFU mL<sup>−1</sup>. The PEC sensor demonstrated outstanding specificity, consistency, replicability, and sustained durability over an extended period, validated through testing on various food samples. The use of ZnWO<sub>4</sub>@g-C<sub>3</sub>N<sub>4</sub> nanocomposite in PEC techniques not only contributes to scientific understanding but also holds practical implications for healthcare industries, emphasizing the potential of this innovative approach in addressing health challenges associated with fungal infections.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"213 ","pages":"Article 113619"},"PeriodicalIF":4.9,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vitamin determination in pet food: A fast and sensitive online solid-phase extraction and heart-cutting 2D liquid chromatography method 宠物食品中维生素的测定：快速灵敏的在线固相萃取和切心二维液相色谱法

IF 4.9 2区化学

Microchemical Journal Pub Date : 2025-04-11 DOI: 10.1016/j.microc.2025.113616

Yang Li , Jitong Wang , Ping Gong , Yuchao Feng , Ting Yao , Xing Gao , Wei Guo , Xue Chen , Jie Cui , Xia Fan

{"title":"Vitamin determination in pet food: A fast and sensitive online solid-phase extraction and heart-cutting 2D liquid chromatography method","authors":"Yang Li , Jitong Wang , Ping Gong , Yuchao Feng , Ting Yao , Xing Gao , Wei Guo , Xue Chen , Jie Cui , Xia Fan","doi":"10.1016/j.microc.2025.113616","DOIUrl":"10.1016/j.microc.2025.113616","url":null,"abstract":"<div><div>An automatic method that combines online solid-phase extraction (online SPE) and heart-cutting two-dimensional liquid chromatography (2D-LC) was developed to determine seven fat-soluble vitamins simultaneously and was validated in pet food. The saponified sample solution was injected directly into our combined online SPE and heart-cut 2D-LC system for enrichment, purification, separation, and quantification. Vitamin A and E isomers were analyzed using pentafluorophenyl (PFP) as the first column. Vitamins D<sub>2</sub> and D<sub>3</sub> were analyzed using C<sub>18</sub> as the second column. The results showed good linearity (R<sup>2</sup> > 0.9964) for the analytes and good reproducibility (RSD < 3 %, n = 3). The limits of quantification for vitamins A, D<sub>2</sub>, and D<sub>3</sub>, and α −, β-, γ-, and δ-E (calculated using S/N = 10:1) were 0.24, 0.09, 0.09, 2.4, 2.4, 2.4 and 2.4 μg/g, respectively. The spiked recoveries were from 80.6 to 104.0 %. The proposed method considerably simplifies the analytical workflow of standard methods for the analysis of fat-soluble vitamins and improves efficiency.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"213 ","pages":"Article 113616"},"PeriodicalIF":4.9,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A dual-mode sensing system based on carbon dots and MnO2 nanosheets with peroxidase-like activity for the detection of alkaline phosphatase and its inhibitors

IF 4.9 2区化学

Microchemical Journal Pub Date : 2025-04-11 DOI: 10.1016/j.microc.2025.113624

Sikai Wang , Xia Long , Lei Wang , Lin Lv , Yue Li , Hongmei Liu , Jingwen He , Xiaojun Luo , Guoqi Zhang , Yan Zhao

{"title":"A dual-mode sensing system based on carbon dots and MnO2 nanosheets with peroxidase-like activity for the detection of alkaline phosphatase and its inhibitors","authors":"Sikai Wang , Xia Long , Lei Wang , Lin Lv , Yue Li , Hongmei Liu , Jingwen He , Xiaojun Luo , Guoqi Zhang , Yan Zhao","doi":"10.1016/j.microc.2025.113624","DOIUrl":"10.1016/j.microc.2025.113624","url":null,"abstract":"<div><div>Alkaline phosphatase (ALP) is widely recognized as an important biomarker for clinical diagnosis. Herein, an innovative fluorometric and colorimetric dual-mode sensing method using MnO<sub>2</sub> nanosheets and carbon dots (CDs) was developed to determine ALP activity. In the presence of H<sub>2</sub>O<sub>2</sub>, MnO<sub>2</sub> nanosheets exhibited remarkable catalytic activity towards the oxidation reaction of catechol to produce <em>o</em>-benzoquinone. The generated <em>o</em>-benzoquinone effectively quenched the fluorescence of CDs through mechanisms of fluorescence resonance energy transfer and photo-induced electron transfer. ALP can catalyze the hydrolysis of L-ascorbic acid-2-phosphate to generate ascorbic acid, and the generated ascorbic acid can reduce MnO<sub>2</sub> nanosheets to Mn<sup>2+</sup>, thereby inhibiting the oxidation of catechol to <em>o</em>-benzoquinone. This leads to both fluorescence recovery of CDs and a decrease in UV absorption. Using the developed fluorometric and colorimetric dual-mode sensing approaches, ALP activity was determined within the range of 0.5–100 U/L and 4–60 U/L, respectively, with corresponding detection limits of 0.13 and 1.18 U/L. Moreover, the proposed sensing approach was successfully applied to detect ALP in human serum samples and screen potential ALP inhibitors, demonstrating its applicability for medical diagnosis and pharmaceutical development applications.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"213 ","pages":"Article 113624"},"PeriodicalIF":4.9,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Automated liquid-phase microextraction into alkyl polyglucoside-based supramolecular solvent: Determination of tetracyclines in saliva

IF 4.9 2区化学

Microchemical Journal Pub Date : 2025-04-11 DOI: 10.1016/j.microc.2025.113617

Aleksei Pochivalov, Mark Filipp Zhavoronok, Aleksandr Purvin, Evgenia Safonova, Andrey Bulatov

{"title":"Automated liquid-phase microextraction into alkyl polyglucoside-based supramolecular solvent: Determination of tetracyclines in saliva","authors":"Aleksei Pochivalov, Mark Filipp Zhavoronok, Aleksandr Purvin, Evgenia Safonova, Andrey Bulatov","doi":"10.1016/j.microc.2025.113617","DOIUrl":"10.1016/j.microc.2025.113617","url":null,"abstract":"<div><div>This work presents for the first time aflow-based approach for theautomation of theliquid-phase microextraction process into environmentally benign alkyl polyglucoside-based supramolecular solvent formed <em>in situ</em> under coacervation with fatty acid. The procedure entails spontaneous phase separation without centrifugation within a mixing chamber of a flow system. Electrolytes were employed to increase density of the aqueous sample phase so that the extract separated on the top of the mixing chamber. It allowed faster and better phase separation (4 min required), dispensing the sample phase, and aspirating the exact volume of the supramolecular solvent phase conveniently and reproducibly. Alkyl polyglucosides C<sub>8</sub>-C<sub>10</sub> and C<sub>8</sub>-C<sub>14</sub>, decyl and coco glucosides were investigated as amphiphiles for automated microextraction. The microextraction of tetracycline antibiotics from saliva samples after deproteinization followed by determination using high-performance liquid chromatography with ultraviolet detection was performed as an example. The supramolecular solvent based on decyl glucoside and 1-hexanoic acid provided good extraction performance (extraction recovery values up to 92 %) along with low viscosity. The limits of detection, calculated from blank tests based on 3<em>σ</em>, were determined to be 10 μg L<sup>-1</sup> for minocycline and oxytetracycline along with 20 μg L<sup>-1</sup> for tetracycline and doxycycline.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"213 ","pages":"Article 113617"},"PeriodicalIF":4.9,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0