Au@Cu2O@SDS composite nanorods for dual-mode sensing of histidine

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal Pub Date : 2025-04-11 DOI:10.1016/j.microc.2025.113621

Chuang Wen, Zhongwei Lin, Yilin Wang

{"title":"Au@Cu2O@SDS composite nanorods for dual-mode sensing of histidine","authors":"Chuang Wen, Zhongwei Lin, Yilin Wang","doi":"10.1016/j.microc.2025.113621","DOIUrl":null,"url":null,"abstract":"<div><div>The content of histidine (His) in urine is one of the important reference indicators for the diagnosis of certain diseases. The research on using nanomaterials as substitutes for organic fluorescent probes and natural enzymes is receiving increasing attention. In this study, Au@Cu<sub>2</sub>O composite nanorods (CNRs) coated with sodium dodecyl sulfate (SDS) (Au@Cu<sub>2</sub>O@SDS CNRs) were prepared. It was found that the obtained Au@Cu<sub>2</sub>O@SDS CNRs emits weak fluorescence at 372 nm when excited at 280 nm, and the presence of His leads to an enhanced fluorescence. On the other hand, the Au@Cu<sub>2</sub>O@SDS CNRs exhibits oxidase-like activity, which can catalyze the oxidation of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) without the need of H<sub>2</sub>O<sub>2</sub> to form blue-colored oxidized TMB (TMBox). However, the addition of His results in a decrease in absorbance at 652 nm. Thus, a fluorescence and colorimetric dual-mode sensing system responsive to His was successfully constructed. Under the optimum conditions, the increase in fluorescence (Δ<em>F</em>) and decrease in absorbance (Δ<em>A</em>) of the system are closely related to His concentration ranging from 0.5-60 μM and 0.3–25 μM with the detection limit of 0.22 and 0.26 μM, respectively. More importantly, the dual-mode sensing system was utilized for the detection of His in human urine samples with satisfactory recovery (90.1 % ∼109.4 %), confirming its good potential for application.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"213 ","pages":"Article 113621"},"PeriodicalIF":4.9000,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchemical Journal","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0026265X25009750","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

The content of histidine (His) in urine is one of the important reference indicators for the diagnosis of certain diseases. The research on using nanomaterials as substitutes for organic fluorescent probes and natural enzymes is receiving increasing attention. In this study, Au@Cu₂O composite nanorods (CNRs) coated with sodium dodecyl sulfate (SDS) (Au@Cu₂O@SDS CNRs) were prepared. It was found that the obtained Au@Cu₂O@SDS CNRs emits weak fluorescence at 372 nm when excited at 280 nm, and the presence of His leads to an enhanced fluorescence. On the other hand, the Au@Cu₂O@SDS CNRs exhibits oxidase-like activity, which can catalyze the oxidation of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) without the need of H₂O₂ to form blue-colored oxidized TMB (TMBox). However, the addition of His results in a decrease in absorbance at 652 nm. Thus, a fluorescence and colorimetric dual-mode sensing system responsive to His was successfully constructed. Under the optimum conditions, the increase in fluorescence (ΔF) and decrease in absorbance (ΔA) of the system are closely related to His concentration ranging from 0.5-60 μM and 0.3–25 μM with the detection limit of 0.22 and 0.26 μM, respectively. More importantly, the dual-mode sensing system was utilized for the detection of His in human urine samples with satisfactory recovery (90.1 % ∼109.4 %), confirming its good potential for application.

Abstract Image

查看原文本刊更多论文

尿液中组氨酸（His）的含量是诊断某些疾病的重要参考指标之一。利用纳米材料替代有机荧光探针和天然酶的研究正受到越来越多的关注。本研究制备了涂有十二烷基硫酸钠（SDS）的 Au@Cu2O 复合纳米棒（CNRs）（Au@Cu2O@SDS CNRs）。研究发现，所获得的 Au@Cu2O@SDS CNR 在 280 纳米波长处激发时会在 372 纳米波长处发出微弱的荧光，而 His 的存在会导致荧光增强。另一方面，Au@Cu2O@SDS CNRs 具有类似氧化酶的活性，无需 H2O2 即可催化 3，3′，5，5′-四甲基联苯胺（TMB）的氧化，形成蓝色的氧化 TMB（TMBox）。然而，加入 His 后，652 纳米波长处的吸光度会降低。因此，我们成功地构建了一个对 His 有反应的荧光和比色双模式传感系统。在最佳条件下，该系统的荧光增加（ΔF）和吸光度降低（ΔA）与 His 浓度密切相关，分别为 0.5-60 μM 和 0.3-25 μM，检测限分别为 0.22 和 0.26 μM。更重要的是，该双模式传感系统用于检测人体尿液样本中的 His，回收率令人满意（90.1 % ∼109.4 %），证实了其良好的应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Microchemical Journal 化学-分析化学

CiteScore

8.70

自引率

8.30%

发文量

1131

审稿时长

1.9 months

期刊介绍： The Microchemical Journal is a peer reviewed journal devoted to all aspects and phases of analytical chemistry and chemical analysis. The Microchemical Journal publishes articles which are at the forefront of modern analytical chemistry and cover innovations in the techniques to the finest possible limits. This includes fundamental aspects, instrumentation, new developments, innovative and novel methods and applications including environmental and clinical field. Traditional classical analytical methods such as spectrophotometry and titrimetry as well as established instrumentation methods such as flame and graphite furnace atomic absorption spectrometry, gas chromatography, and modified glassy or carbon electrode electrochemical methods will be considered, provided they show significant improvements and novelty compared to the established methods.