Microchemical Journal最新文献

{"title":"pH sensitive indicator film based intelligent packaging systems of perishables: Developments and challenges of last decade","authors":"Anika Panwar ,&nbsp;Satish Kumar ,&nbsp;Atul Dhiman ,&nbsp;Vikas Kumar ,&nbsp;Dharmesh Gupta ,&nbsp;Ajay Sharma","doi":"10.1016/j.microc.2024.111732","DOIUrl":"10.1016/j.microc.2024.111732","url":null,"abstract":"<div><div>Consumers’ demand for novel and non-destructive systems to assess food freshness in real time has led to the development of halochromic or pH-sensitive dyes based freshness indicators (FI) as affordable technique for intelligent monitoring of perishable food. However, the genuine concern regarding the toxicity and deleterious health effect of incorporating conventional synthetic dyes in commercially available halochromic freshness indicators (i.e. fresh tag, checkpoint, and sensorQ) and their sole purpose of monitoring the freshness status of muscle foods (i.e. fish, meat, beef, and shrimp) have led to the revolutionary idea of developing a non-toxic, chemical-free, and sustainable FI based on natural pH-sensitive dyes and polymer matrix. This review delves into significant advances in the chemistry, composition, production, food interaction, stabilization and broad applications of pH-sensitive dyes in the food sector. Unfortunately, the pH-sensitive dyes have reported limited applications in fresh fruit and vegetable industry due to challenges like erratic fluctuations in pH as influenced by factors i.e. high respiration, faster ripening, and environmental changes, inability to distinguish ripening from spoilage, and complex standardization process for specific commodities. These issues merit the demand for further investigations to ensure the utilization of these smart monitoring systems in the modern industry for perishables. The use of FI can potentially play a significant role in reforming food industry, enhance consumer safety, reduce the risk of foodborne illnesses, and aid in the development of a more efficient and sustainable food supply system. Exploration of new natural ingredients and high sensitivity indicators for both animal and plant food products shall drive the innovations in FI for the food industry.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111732"},"PeriodicalIF":4.9,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorine and boron co doped porous carbon materials as an electrochemical platform in trace-level sodium nitrite sensing","authors":"Nuerbiya Yalikun,&nbsp;Hongzhou gong,&nbsp;Chaofan Liu,&nbsp;Yingshuang Zhang","doi":"10.1016/j.microc.2024.111769","DOIUrl":"10.1016/j.microc.2024.111769","url":null,"abstract":"<div><div>Sodium nitrite (NaNO₂) is a common chemical oilfield tracer in the field of reservoir geological analysis. A simple, sensitive, and accurate electrochemical detection method for detection of NaNO₂ has been studied. The electrode carbon porous material was based on coal tar pitch n-pentane insoluble extractants (CTPNI) and doped with the elements of fluorine (F) and boron (B) and labeled as FB-CTPNI-PC and an electrochemical sensor modified with FB-CTPNI-PC material was constructed for rapid detection of NaNO₂. The characterization results of SEM and TEM indicate that this material has certain number of pore structures, also the BET analysis showed that has main pore size distribution is around the range of 2–20 nm, with abundant mesoporous structure. Moreover, the modified electrode has a good electrocatalytic effect on NaNO₂. The electrochemical reaction mechanism of NaNO₂ on the surface of the modified electrode was a diffusion-controlled process involving protons. The detection range of NaNO₂ can reach 0.1 mM–9.5 mM. When the S/N is 3, the sensitivity is 219.8592 A·L·cm²·mol⁻¹ and the detection limit (LOD) is 0.011 μM. The constructed electrochemical sensor has been applied to the rapid detection of NaNO₂ in real oilfield water, with recovery rates ranging from 99.7 % to 103.4 %. It is also roughly consistent with the results of ion chromatography (IC) in detecting NaNO₂ in oilfield water, indicating that the sensor has high accuracy. This has provided a new prospect for oil filed tracer and detection technologies.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111769"},"PeriodicalIF":4.9,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142357863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Fourier infrared spectroscopy for noninvasive diagnosis of diabetes mellitus: Analysis and prospects for application based on blood, urine and saliva samples","authors":"Jing Yin ,&nbsp;Guangwei Wang ,&nbsp;Xianwen Zhang ,&nbsp;Zhushanying Zhang ,&nbsp;Yuan Gao ,&nbsp;Wenchao Pan ,&nbsp;Huimin Cao","doi":"10.1016/j.microc.2024.111764","DOIUrl":"10.1016/j.microc.2024.111764","url":null,"abstract":"<div><div>Diabetes, a prevalent metabolic disorder, is typically diagnosed through the analysis of blood samples. Biochemical assays predicated on blood analysis, while affording a high degree of accuracy, inherently involve an invasive procedure and present logistical inconveniences for patients, thereby necessitating the exploration of alternative diagnostic modalities. In recent years, Fourier transform infrared spectroscopy (FTIR) has emerged as a promising tool for the rapid, sensitive, cost-effective, and non-invasive investigation of diagnostic modalities for diabetes mellitus, offering a novel avenue for advancing diabetes management strategies. This review offers a comprehensive summary of the current research landscape in recent years, focusing on the application of FTIR analysis for the detection of biological body fluids in the context of diabetes diagnosis. It encompasses an introductory overview of the research methodology employed for diagnosing diabetes through the analysis of three distinct body fluids, along with a forward-looking perspective on its potential implications and applications. The review commences with a comprehensive overview of the introduction and utilization of blood, urine, and saliva samples in the context of diabetes research. A meticulous comparison of existing research findings underscores the efficacy of Fourier Transform Infrared (FTIR) spectroscopy as a discriminating tool for differentiating body fluid samples derived from diabetic patients and those from healthy individuals. This advanced technique exhibits remarkable capability in identifying and distinguishing among diabetic patients by precisely quantifying the intensity and frequency of characteristic spectral peaks corresponding to specific biochemical components present within body fluids. FTIR spectroscopy holds promising clinical applications for diabetes diagnosis in body fluids, aiming to enable early detection and non-invasive monitoring via rapid biomarker identification.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111764"},"PeriodicalIF":4.9,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142357859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A sensitive Fe3O4@MnO2 nanocatalyst designed for visual determination of total antioxidant capacity in fruit juice","authors":"Hong Chen ,&nbsp;Junyan Liu ,&nbsp;Mengna Wan ,&nbsp;Shangyi Zhao ,&nbsp;Xiaolong Li ,&nbsp;Chuan Liu ,&nbsp;Xuanping Zhao ,&nbsp;Junjie Li ,&nbsp;Qingzhen Ma ,&nbsp;Cuixiang Li ,&nbsp;Xueqin Wang","doi":"10.1016/j.microc.2024.111709","DOIUrl":"10.1016/j.microc.2024.111709","url":null,"abstract":"<div><div>Fruit juice is kind of popular beverage food worldwidely, and its total antioxidant capacity (TAC) is generally considered as an important index for people’s rational diet, thus accurate evaluation of its TAC is essential for human health guidance. Herein, we constructed a sensitive Fe₃O₄@MnO₂ nanocatalyst that was delicately cored with magnetite nanoparticles (Fe₃O₄ NPs) and encapsulated with manganese dioxide (MnO₂) nanoshells for fast and accurate TAC colorimetric analysis in fruit juice. The data showed the fabricated Fe₃O₄@MnO₂ nanocatalysts performed with remarkable oxidase mimetic activities and could catalyze oxidization of colorimetric substrate without additional H₂O₂, and the limit of detection (LOD) was about 0.39 μM for ascorbic acid (AA) evaluation. Applied in commercial fruit juice products, the fabricated Fe₃O₄@MnO₂ nanocatalysts showed a reliable and visual assessment for the accurate TAC analysis. In addition, the fabricated Fe₃O₄@MnO₂ nanocatalysts could be easily recycled due to their intrinsic superparamagnetic property. Together, an intellegent Fe₃O₄@MnO₂ nanocatalyst was well developed for visual TAC analysis in fruit juice with convenient applications in future.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111709"},"PeriodicalIF":4.9,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142357860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescent aptasensor based on competitive recognition strategies and point-of-care testing system for brain natriuretic peptide detection","authors":"Ziyue Qin,&nbsp;Shouzhe Deng,&nbsp;Jiawang Wang,&nbsp;Jie Fu,&nbsp;Fangying Xiong,&nbsp;Qiya Gao,&nbsp;Shuang Li","doi":"10.1016/j.microc.2024.111742","DOIUrl":"10.1016/j.microc.2024.111742","url":null,"abstract":"<div><div>Brain natriuretic peptide (BNP) is the preferred biomarker for clinical analysis, widely used in early screening and prognostic monitoring of cardiovascular and neurological diseases. Due to the low concentration and short half-life of BNP in the blood, its sensitive detection remains a challenge that must be overcome. With the rapid development of molecular diagnosis and point-of-care testing (POCT), developing a BNP biosensor with high sensitivity, good stability, accuracy, speed, and low-cost is of great significance for clinical applications and emergency diagnosis. However, BNP in human blood exists in various forms, and traditional fluorescence sensors may lead to overestimation of the concentration. In this work, we constructed an “on” fluorescent aptasensor based on competitive recognition strategy for quantitative detection of BNP, and built a fluorescence sensing detection system based on smartphone to achieve rapid on-site detection. We designed a specific aptamer labeled with carboxyfluorescein (FAM) with a simple structure and high biological affinity for selective capture of BNP, and utilized the large specific surface area and excellent fluorescence quenching effect of carboxylated graphene oxide (GO-COOH) as a carrier and quencher for the aptasensor. By optimizing experimental parameters such as aptamer and GO-COOH concentrations, as well as quenching/recovery time, linear sensitive detection of BNP was achieved in the concentration range of 0.01 ng/mL–10 ng/mL, with a detection limit as low as 10 pg/mL, good specificity, and excellent application potential in artificial serum spiking experiments. Additionally, a stable and sensitive fluorescence signal collection system has been developed to meet the needs of portable detection, breaking through the usage environment of traditional fluorescence detection equipment and filling the gap of portable fluorescence biosensing. In summary, we have creatively proposed a fluorescent aptasensor that enables rapid and sensitive detection of BNP and the development of low-cost fluorescent biosensors. The combination with POCT has shown broad application prospects and also provides some ideas for the fluorescence detection of other protein biomarkers.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111742"},"PeriodicalIF":4.9,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intelligent and automatic characterization of ignitable liquid residues by using total ion spectrum and machine learning","authors":"Augosto Asor Misolas,&nbsp;Marta Ferreiro-González,&nbsp;Miguel Palma","doi":"10.1016/j.microc.2024.111757","DOIUrl":"10.1016/j.microc.2024.111757","url":null,"abstract":"<div><div>The identification and classification of ignitable liquid residues (ILRs) are among the most challenging steps in a fire investigation. Traditionally, fire debris samples are analyzed by GC–MS according to ASTM E1618 guidelines, where ILRs are determined via chromatographic pattern comparison with reference samples and classified according to ASTM classes. This complex and partially subjective task relies heavily on an experienced analyst and does not lend itself to automation. This study aims to provide alternative methods for the automatic data classification based on the total ion spectrum (TIS) combined with machine learning (ML) algorithms.<!--> <!-->We evaluated and compared thermal desorption of ILRs from activated charcoal strips (ACS samples) and direct headspace analysis of different fire debris samples to develop automated discrimination of different ILRs. Hierarchical cluster analysis (HCA) and supervised ML algorithms like linear discriminant analysis (LDA), support vector machines (SVM), and random forest (RF) were employed. The samples tended to initially cluster according to the presence/absence of ILRs followed by the type of ILR. Three datasets were tested (1) ILRs presence/absence, (2) ILR classification, and (3) combination of both. For ACS samples, all the datasets achieved 100% classification accuracies. For direct headspace analysis, dataset (1) resulted in 100% accuracies when the three algorithms were used. Models using dataset (2) and (3) achieved less than 100%. A prototype web-based application for the automatized discrimination of ACS samples was created using the SVM model. The application of ML algorithms demonstrated the effectiveness of the developed methods as supplementary to traditional ASTM method. These methods provide significant potential for automating the classification of ILRs therefore they can be useful to the experts in interpreting fire debris data.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111757"},"PeriodicalIF":4.9,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142326384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous determination of florfenicol, its metabolite and three fluoroquinolone residues in poultry muscles via high-performance liquid chromatography with fluorescence detection","authors":"Lu Hong ,&nbsp;Yun Liu ,&nbsp;Yawen Guo ,&nbsp;Pengfei Gao ,&nbsp;Hao Ding ,&nbsp;Junjie Xu ,&nbsp;Xing Xie ,&nbsp;Kaizhou Xie","doi":"10.1016/j.microc.2024.111754","DOIUrl":"10.1016/j.microc.2024.111754","url":null,"abstract":"<div><div>A high-performance liquid chromatography with fluorescence detection (HPLC<em>–</em>FLD) procedure was developed and refined to detect florfenicol (FF), its metabolite florfenicol amine (FFA), and three fluoroquinolone (FQ) residues present in poultry muscles concurrently. Sample pretreatment was conducted through liquid–liquid extraction (LLE). Ethylenediaminetetraacetic acid (EDTA), 90 % acetonitrile and acetonitrile were used as extraction solvents. The stationary phase employed was an XBridge BEH C18 column (5 μm, 4.6 mm × 150 mm), and the mobile phase consisted of a 0.01 mol/L solution of sodium dihydrogen phosphate and acetonitrile (65:35, V/V); the former contained 0.005 mol/L sodium dodecyl sulfate and 0.1 % triethylamine (pH adjusted to 4.8 with phosphoric acid). The limits of detection (LODs) and limits of quantification (LOQs) were 0.03–1.5 µg/kg and 0.1–5.0 μg/kg, respectively. The linear relationships were satisfactory within the corresponding concentration ranges, with determination coefficients (R²) ≥ 0.9997. The mean recoveries ranged from 70.34 to 94.98 %, and the intraday relative standard deviations (RSDs) and interday RSDs were 1.46–6.24 % and 1.60–7.31 %, respectively. This verified method is applicable and reliable for analyzing actual samples.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111754"},"PeriodicalIF":4.9,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142326387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilizing HPLC-DAD, multivariate analysis, molecular docking, and in vitro validation methods to investigate phenolic compounds with potential antidiabetic properties in Fraxinus mandshurica","authors":"Jianjin Guo ,&nbsp;Chi-Tang Ho ,&nbsp;Naisheng Bai","doi":"10.1016/j.microc.2024.111771","DOIUrl":"10.1016/j.microc.2024.111771","url":null,"abstract":"<div><div>This comprehensive study utilized response surface methodology (RSM) to optimize extraction conditions for total flavonoids and phenols from <em>F. mandshurica</em>. Optimal conditions for flavonoids were 75 % ethanol, 63 °C, and 84 min, yielding 14.98 ± 0.13 mg/g. For phenols, it was 70 % ethanol, 66 °C, and 87 min, resulting in 26.04 ± 0.22 mg/g. UPLC-Q-TOF-MS/MS identified 68 constituents in <em>F. mandshurica</em>, categorized into groups. HPLC-DAD established a method for quantitative analysis of 12 target compounds. Geographical distribution analysis highlighted the northeast region as the main production area. Fingerprinting of 30 samples and multivariate analysis identified five active compounds. Hierarchical cluster analysis (HCA) showed differences in active compounds based on origin. In <em>vitro</em> antidiabetic evaluation demonstrated α-glucosidase inhibitory effects. Molecular docking studies were conducted between 12 active compounds and the antidiabetic-related protein 8A27. Esculetin had the highest binding affinity. The study examined extraction methods, analyzed samples, and explored antidiabetic effects, providing data support and insights for <em>F. mandshurica</em> as a natural industrial product and for extracts and active compounds as antidiabetic sources.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111771"},"PeriodicalIF":4.9,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and multipurpose applications of nitrogen-sulfur doped carbon dots fluorescent probe for Ga3+ and riboflavin (VB2) detection","authors":"Chan Huang,&nbsp;Le Liang,&nbsp;Yong-qing Liu,&nbsp;Mei Han,&nbsp;Xue-lin Wang,&nbsp;Xiao-hua Cai,&nbsp;Guang-long Zou","doi":"10.1016/j.microc.2024.111759","DOIUrl":"10.1016/j.microc.2024.111759","url":null,"abstract":"<div><div>Designing versatile carbon quantum dots (CDs) with excellent sensitivity and unique function as efficient fluorescent probe for detection of specific substance from cheap and readily available substrates is still a crucial challenge. A novel nitrogen-sulfur doped carbon dots (N, S-CDs) with blue-green fluorescent emission derived from lactose and 4-amino-3-hydroxy-1-naphthalenesulfonic acid (AHNS) was facilely synthesized by one-step hydrothermal process. The obtained N, S-CDs with graphene structure exhibited good water solubility, excellent biocompatibility, satisfactory stability and striking optical properties. The surface of N, S-CDs is modified by various functional groups which are helpful to combine with Ga³⁺ in the form of complexes N, S-CDs-Ga³⁺ to considerably enhance the fluorescence emission peak of N, S-CDs, further improving the selectivity and sensitivity of Ga³⁺ detection with the detection limit of 0.133 μM. At the meantime, N, S-CDs can cause yellow fluorescence quenching of riboflavin (VB₂) with the detection limit of 0.121 μM under the concentration range from 1.000-20.000 μM. The possible mechanism is the functional groups at the surface of N, S-CDs combined with the hydroxyl, amino and carbonyl group of VB₂ by protonation and hydrogen bonds. Further investigations for practical applications demonstrated that N, S-CDs has good prospects in Ga³⁺ananlysis and detection of VB₂ in food. Moreover, N, S-CDs can be used to determine whether there is Ga³⁺ in transferrin, which can indirectly inhibit the growth of cancer cells through the influence on transporting iron ion. Therefore, it is profitable for preliminary diagnosis and treatment of cancer cells in the field of biomedicine.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111759"},"PeriodicalIF":4.9,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142357868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel sulfonylurea fluorescence probes for antiproliferative activity, detection and imaging of fluoride ions in living cells","authors":"Thi–Dan Thach ,&nbsp;Le–Kim–Thuy Nguyen ,&nbsp;T. Duy–Nguyen Nguyen ,&nbsp;T. My–Thao Nguyen ,&nbsp;Van–Su Dang ,&nbsp;Dinh Tri Mai ,&nbsp;Van–Dung Le ,&nbsp;Chi–Hien Dang ,&nbsp;Quynh Nguyen Nhu Le ,&nbsp;Phuong Thuy Viet Nguyen ,&nbsp;Minh Nam Nguyen ,&nbsp;Van Kieu Thuy Nguyen ,&nbsp;Nguyen–Nguyen Pham–Tran ,&nbsp;Thanh–Danh Nguyen","doi":"10.1016/j.microc.2024.111760","DOIUrl":"10.1016/j.microc.2024.111760","url":null,"abstract":"<div><div>In this study, four novel triphenylpyrazoline 4-toluenesulfonylureas (PYSUs) were synthesized to develop potential anticancer drugs and fluorescent probes for the detection of F⁻ ions. The compounds were synthesized via a two-step microwave-assisted method. The antiproliferative activity of the PYSUs was tested against two cancer cell lines: HepG2 and MDA-MB-231. Among the compounds, <strong>3a</strong> exhibited strong activity against both cell lines, while <strong>3b</strong> showed strong activity against MDA-MB-231 cells but weaker inhibition of HepG2 cells. Furthermore, compound <strong>3b</strong> was identified as the best fluorescent probe, with an increase in emission intensity in the presence of F⁻ ions from tetrabutylammonium fluoride (TBAF). In DMSO, the emission wavelength was found to be 518 nm (green) with an excitation wavelength of 469 nm. The limit of detection (LOD) was calculated to be 0.269 mM in the linear range of 5–10 mM. The interaction mechanism between F⁻ ions and compound 3b was further explored via DFT/TDDFT calculations. Additionally, fluorescent imaging of TBAF-incubated MDA-MB-231 and HepG2 cells confirmed the potential of PYSUs as fluorescent sensors for F⁻ ions in living cancer cells.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111760"},"PeriodicalIF":4.9,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142326391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}