Veterinary Sciences最新文献

{"title":"Disseminated <i>Neospora caninum</i> Encephalomyelitis and Myositis in a 3-Month-Old Cane Corso Puppy.","authors":"Abigail L English, Joshuah B Klutzke, Stephanie A Thomovsky, Nobuko Wakamatsu","doi":"10.3390/vetsci11110544","DOIUrl":"10.3390/vetsci11110544","url":null,"abstract":"<p><p>We present a case of fatal disseminated neosporosis in a 3-month-old Cane Corso puppy. The puppy presented to the Purdue Veterinary Hospital emergency service with a 1-week history of progressive left hindlimb lameness. On presentation, the puppy was nonambulatory paraparetic. Signs progressed over two days to nonambulatory tetraparesis, and decreased to absent limb reflexes, cranial nerve deficits, and dull mentation. Blood work showed eosinophilia, elevated ALT, and extremely elevated creatine kinase at 36,000 IU/L (reference interval 22-491 IU/L). Two days after diagnostics were performed, the puppy experienced acute cardiac arrest, and the body was submitted for necropsy. At necropsy, skeletal muscle in all four limbs was diffusely pale tan. Tan streaks were disseminated through the diaphragm, abdominal wall, and myocardium. Histologically, Skeletal myocytes and cardiomyocytes were frequently degenerative, with abundant lymphohistiocytic inflammation and fibrosis. White matter within the brain and spinal cord was inflamed and contained frequent dilated myelin sheaths and spheroids. A few protozoal cysts were within the brain and skeletal myocytes. Antemortem antibody titers were positive for IgG against <i>Neospora caninum</i> (dilution titer of 1:4096, baseline 1:32), confirming protozoal cysts as <i>Neospora caninum</i>. Disseminated neosporosis is an uncommon but important clinical differential for ascending paresis in young dogs.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11599034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142732993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunologic Effects of a Novel Bovine Lactoferrin-Derived Peptide on the Gut and Clinical Perspectives.","authors":"Haiyue Cui, Huan Yang, Xiaoxi Qi, Yang Zhao, Tianle Huang, Liguang Miao","doi":"10.3390/vetsci11110545","DOIUrl":"10.3390/vetsci11110545","url":null,"abstract":"<p><p>Bovine lactoferrin is a natural iron-binding glycoprotein known for its antimicrobial, antiviral, antitumor, anti-inflammatory, and immunomodulatory properties. In this study, we artificially recombined a fragment of bovine lactoferrin with immunomodulatory and antimicrobial properties to create a novel peptide named LF-MQL. The primary objective was to investigate the effects of LF-MQL on the intestinal tract and immune cells in animals. First, we assessed the in vitro activation effects of LF-MQL on mouse peritoneal macrophages. The results indicated that LF-MQL enhanced the macrophage phagocytic activity and increased IL-1β mRNA expression without significantly affecting IL-6 mRNA levels. Next, we examined the effects of LF-MQL on mucosal immunity by administering LF-MQL orally at doses of 300 mg/kg, 30 mg/kg, and 3 mg/kg to mice. The results demonstrated that different doses of LF-MQL modulated IL-6 and IL-10 mRNA levels in the small intestine. Low doses enhanced the intestinal immune response, while higher doses reduced the inflammatory response. In conclusion, LF-MQL exerts immunomodulatory effects rather than simply boosting immune activity in animal models.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11599047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142732303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-Genome Analysis of Porcine Epidemic Diarrhea Virus from Yunnan, China.","authors":"Runting Zhang, Gefen Yin, Yunhua Wang, Yongneng Li, Xinxian Wang, Junlong Bi, Guishu Yang, Kaixing Qu, Libo Gao","doi":"10.3390/vetsci11110548","DOIUrl":"10.3390/vetsci11110548","url":null,"abstract":"<p><p>Porcine epidemic diarrhea virus (PEDV) is a member of the genus α-coronavirus and causes severe diarrhea in piglets, leading to enormous economic losses in the pig industry. To understand the epidemic variation of PEDV strains in Yunnan province, three PEDV strains (YN2021, YNLP 2022, and YNBS 2022) and one commercially available attenuated vaccine strain (Attenuated AJ1102-R) that were previously isolated were sequenced and compared with the representative PEDV strains. NJ phylogenetic analysis showed that YN2021 strain and Attenuated CV777 strain were clustered into GI-b subtype, while YNLP 2022 and YNBS 2022 belong to GII-b subtype, accompanying ZJCZ4 and Attenuated AJ1102-R. RDP analysis revealed that YNLP 2022 was a genome recombination from both GII-b strain PEDV-7C and GII-a strain YN1, of which the recombination region is in the range nt4994-7605. YNBS 2022 strain was another recombination originated from GII-b subtype strain 17GXZC-1ORF3c and GII-a subtype strain PEDV-CHZ, of which the counterpart is in the range nt16399-22326. The Yunnan strain of PEDV was analyzed for the first time from the whole-genome perspective, and comprehensive analysis showed that the Yunnan strains have high genetic variation. This study may shed new light on the current PEDV infections in Yunnan and pave the way toward further control of PEDV infections.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11599152/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Retrospective Report on the Infestation and Distribution of Chiggers on an Endemic Rodent Species (<i>Apodemus latronum</i>) in Southwest China.","authors":"Qiao-Yi Liu, Xian-Guo Guo, Rong Fan, Wen-Yu Song, Pei-Ying Peng, Ya-Fei Zhao, Dao-Chao Jin","doi":"10.3390/vetsci11110547","DOIUrl":"10.3390/vetsci11110547","url":null,"abstract":"<p><p>Chiggers are the exclusive vector of <i>Orientia tsutsugamushi</i>, which is the causative agent of scrub typhus. Based on the field surveys in southwest China between 2001 and 2022, this paper retrospectively reported the chigger infestation and distribution on the large-eared field mouse (<i>Apodemus latronum</i>), an endemic rodent species in the region, for the first time. A total of 933 chiggers were collected from 501 mice, and these chiggers were identified as 2 families, 8 genera, and 61 species. The overall infestation prevalence (<i>P_M</i>) and mean abundance (<i>MA</i>) of <i>A. latronum</i> with chiggers reached 19.76% and 1.86 mites/per mouse, respectively. The chigger infestation indices on adult <i>A. latronum</i> (<i>P_M</i> = 38.28%, <i>MA</i> = 5.11) were higher than those on juvenile mice (<i>P_M</i> = 12.63%, <i>MA</i> = 0.97) with <i>p</i> < 0.01, showing an age bias of infestation. The relative fatness (<i>K</i>) was used to reflect the nutrition status of the mouse host. The mouse hosts with good nutrition (<i>K</i> = 3.4 ± 0.89 g/cm³) harbored fewer chiggers than the hosts with poor nutrition (<i>K</i> = 2.2 ± 0.90 g/cm³) (<i>p</i> < 0.01). The infestation indices of chiggers on <i>A. latronum</i> obviously fluctuated along different altitude gradients (<i>p</i> < 0.01). With the increase in altitudes, the <i>β</i> diversity of the chigger community showed a gradually increasing tendency. The spillover chord diagram, which was based on indices of PAC (potential for apparent competition), revealed high spillover potentials of dominant chigger species dispersing from high altitude gradients to the lowest one. The chigger abundance was positively correlated with the mean monthly temperature (tmp), mean monthly humidity (hum), the mean monthly precipitation (pre), and the human footprint (hfp), and it was negatively correlated with the altitude (ele) (<i>p</i> < 0.05). The temperature and humidity are the most important factors which influence the chigger infestation.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598831/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142732917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Seroprevalence Study of Feline Leukemia and Feline Immunodeficiency Infections Among Cats in Algiers (Algeria) and Associated Risk Factors.","authors":"Fatima Yahiaoui, Moustafa Kardjadj, Meriem Hind Ben-Mahdi","doi":"10.3390/vetsci11110546","DOIUrl":"10.3390/vetsci11110546","url":null,"abstract":"<p><p>Feline leukemia and feline immunodeficiency virus infections are two widespread diseases that affect the health of domestic cats all over the world. In Algeria, despite the existence of a large population of cats, there are no published data regarding the epidemiological status of feline retroviruses. Therefore, the objective of the present study was to estimate the seroprevalence of FIV and FeLV infections in cats in Algiers and to assess the main factors associated with the infection. A cross-sectional study was conducted in which 71 cats were sampled from several private veterinary clinics, and their obtained sera were tested using an immunochromatography analysis (SNAP Duo FIV/FeLV Test; Virbac Laboratories, France). The seroprevalence was about 32.39% and 22.53% for FIV and FeLV, respectively. Overall, 11.26% were positive for both infections. The present study showed that FIV seropositivity was associated with a younger age and health status (<i>p</i> < 0.05), while FeLV seropositivity was associated with the sex, reproductive status, housing, age, and health status of the cats. This preliminary study, which utilized rapid tests, provides important initial data revealing a relatively high prevalence of retroviruses in cats in Algeria. The results underscore the urgent need for implementing both preventive and management measures to control the spread of these retrovirus diseases. However, to enhance the reliability of future findings, it is essential to expand the sample size and incorporate reference diagnostic methods.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Application of a TaqMan RT-qPCR for the Detection of Foot-and-Mouth Disease Virus in Pigs.","authors":"Changying Dong, Xingyu Xiao, Meiqi Wang, Yajuan Sun, Hui Jin, Yongzhe Zhang, Hongri Zhao, Qianyue Cao, Yanran Yang, Rui Yin","doi":"10.3390/vetsci11110541","DOIUrl":"10.3390/vetsci11110541","url":null,"abstract":"<p><p>The global livestock industry is facing a serious threat from a widespread foot-and-mouth disease virus (FMDV) epidemic. The timely detection of FMDV can significantly mitigate its harmful effects. This study aimed to establish and evaluate a TaqMan fluorescence quantitative PCR assay to assess its sensitivity, specificity, reproducibility, and stability. The standard curve equation range is 6.43 × 10⁹-6.43 × 10¹ copies/µL, with an R² value of 0.996 and a standard curve equation of y = -3.586x + 36.245. The method successfully detected 64.3 copies/µL of the target gene for FMDV and exhibited high specificity for FMDV. Repeatability tests demonstrated low coefficients of variation within and between groups (<2%), indicating good reproducibility. The clinical samples analyzed using this method showed results consistent with those of the SYBR Green I RT-qPCR assay, confirming the reliability of the method. Overall, the developed test method displayed high sensitivity, specificity, reproducibility, and stability, making it suitable for the rapid diagnosis of foot-and-mouth disease in clinical settings.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598863/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Longitudinal Study of Transcriptomic Changes Occurring over Six Weeks of CHOP Treatment in Canine Lymphoma Identifies Prognostic Subtypes.","authors":"Miles W Mee, Sydney Faulkner, Geoffrey A Wood, J Paul Woods, Dorothee Bienzle, Brenda L Coomber","doi":"10.3390/vetsci11110540","DOIUrl":"10.3390/vetsci11110540","url":null,"abstract":"<p><p>The majority of canine lymphoma patients treated with the standard of care, the CHOP chemotherapy protocol, initially achieve remission but eventually relapse with a multi-drug-resistant phenotype. This study assesses gene expression profiles of canine lymphoma tumor cell populations using RNA-Seq data from 15 matched patient samples taken prior to treatment and again six weeks into treatment with CHOP. Two distinct clusters were present in the t-SNE dimensionality reduction of the gene expression profiles. There was a significant difference in progression-free survival (PFS) between the cluster groups, with a median of 43.5 days in a group of six patients and 185 days in another group of nine patients. Comparing the group with shorter PFS to the group with longer PFS, we identified 265 significantly enriched GO:BP terms in 3874 significantly up-regulated genes and 740 significantly enriched GO:BP terms in 3236 significantly down-regulated genes. Comparing the six-week timepoint against the initial timepoint, in the group with longer PFS, we identified 277 significantly enriched GO:BP terms in 413 significantly up-regulated genes and 222 significantly enriched GO:BP terms in 267 significantly down-regulated genes. In the group with shorter PFS, we only identified 27 significantly differentially expressed genes, for this comparison. We found DNA damage response genes to be enriched in the down-regulated genes in both comparisons. These results identify and characterize two transcriptionally distinct groups of canine lymphoma patients with significantly different responses to CHOP chemotherapy.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11599011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142732426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling Antimicrobial Resistance in <i>Mycoplasma hyopneumoniae</i>: Genetic Mechanisms and Future Directions.","authors":"Raziallah Jafari Jozani, Mauida F Hasoon Al Khallawi, Darren Trott, Kiro Petrovski, Wai Yee Low, Farhid Hemmatzadeh","doi":"10.3390/vetsci11110542","DOIUrl":"10.3390/vetsci11110542","url":null,"abstract":"<p><p>Antimicrobial resistance (AMR) in <i>Mycoplasma hyopneumoniae</i>, the causative agent of Enzootic Pneumonia in swine, poses a significant challenge to the swine industry. This review focuses on the genetic foundations of AMR in <i>M. hyopneumoniae</i>, highlighting the complexity of resistance mechanisms, including mutations, horizontal gene transfer, and adaptive evolutionary processes. Techniques such as Whole Genome Sequencing (WGS) and multiple-locus variable number tandem repeats analysis (MLVA) have provided insights into the genetic diversity and resistance mechanisms of <i>M. hyopneumoniae</i>. The study underscores the role of selective pressures from antimicrobial use in driving genomic variations that enhance resistance. Additionally, bioinformatic tools utilizing machine learning algorithms, such as CARD and PATRIC, can predict resistance traits, with PATRIC predicting 7 to 12 AMR genes and CARD predicting 0 to 3 AMR genes in 24 whole genome sequences available on NCBI. The review advocates for a multidisciplinary approach integrating genomic, phenotypic, and bioinformatics data to combat AMR effectively. It also elaborates on the need for refining genotyping methods, enhancing resistance prediction accuracy, and developing standardized antimicrobial susceptibility testing procedures specific to <i>M. hyopneumoniae</i> as a fastidious microorganism. By leveraging contemporary genomic technologies and bioinformatics resources, the scientific community can better manage AMR in <i>M. hyopneumoniae</i>, ultimately safeguarding animal health and agricultural productivity. This comprehensive understanding of AMR mechanisms will be beneficial in the adaptation of more effective treatment and management strategies for Enzootic Pneumonia in swine.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598952/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Clinical Application of Dynamic Contrast-Enhanced MRI in Canine Masses of Mesenchymal and Epithelial Origin: A Preliminary Case Series.","authors":"Chang-Hyeon Cho, Jaehwan Kim, Kidong Eom","doi":"10.3390/vetsci11110539","DOIUrl":"10.3390/vetsci11110539","url":null,"abstract":"<p><p>Evaluating masses of mesenchymal and epithelial origin accurately using computed tomography (CT) has several limitations in dogs. This study aimed to present dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) parameters to aid in improving the diagnostic accuracy for masses of mesenchymal and epithelial origin in veterinary medicine. Four dogs diagnosed with benign and malignant soft tissue sarcoma (STS), cholesteatoma, or squamous cell carcinoma underwent CT, conventional MRI, and DCE-MRI. K^trans is a quantitative DCE-MRI parameter representing vascular permeability and tissue perfusion and is related to the potential for malignancy. Hemangiopericytomas (Grade II, STS) showed a higher K^trans than normal muscle tissue and myxosarcoma (Grade I, STS). Squamous cell carcinoma (a malignant epithelial tumor) also showed a higher K^trans than normal muscle tissue and cholesteatoma (a mass originating from keratinized squamous epithelium). These results suggest that higher K^trans values may indicate a greater likelihood that a lesion is more malignant. In conclusion, K^trans might be useful as a biomarker for evaluating the malignancy of a mass and as an indicator of lesion characteristics in dogs.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neutralizing VHH Antibodies Targeting the Spike Protein of PEDV.","authors":"Li Zhang, Wei Miao, Mo Zhou, Miao Lin, Changyao Fu, Zhi Wu, Xinnuo Lei, Jialong Xu, Shinuo Cao, Shanyuan Zhu","doi":"10.3390/vetsci11110533","DOIUrl":"10.3390/vetsci11110533","url":null,"abstract":"<p><p>Porcine epidemic diarrhea virus (PEDV) is a highly contagious coronavirus that infect pigs' intestinal epithelial cells, causing high morbidity and mortality. Due to the rapid mutation of PEDV, vaccine efficacy is uncertain, prompting exploration of alternative treatments. Nanobodies, also known as variable heavy chain domains of heavy chain-only antibodies (VHHs), offer significant potential in biomedical applications due to their small size and high specificity. In this study, yeast two-hybrid technology was employed to screen for eight specific VHH sequences targeting the PEDV S protein from a synthetically constructed nanobody yeast library. The VHH genes were then cloned into expression plasmids for recombinant protein production, and the resulting VHHs (termed PEDV S-VHHs) were purified. Indirect immunofluorescence assay (IFA) and Western blotting analysis confirmed that these VHHs specifically bind to both PEDV and its S protein. Neutralization assays demonstrated that seven PEDV S-VHHs exhibited potent neutralizing activity against PEDV. Additionally, a combination of these seven antibodies showed enhanced antiviral effects. Preliminary predictions were also made regarding the binding sites between these VHHs and PEDV. The PEDV S-VHHs described in this study hold potential as candidates for the prevention and treatment of PEDV infection.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598873/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142732863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}