Silvia Carta, Riccardo Chessa, Roberto Rubattu, Anna Nudda, Gianni Battacone
{"title":"The Use of Grape By-Products as a Feed Additive Enhances the Oxidative Stability of Rabbit Meat.","authors":"Silvia Carta, Riccardo Chessa, Roberto Rubattu, Anna Nudda, Gianni Battacone","doi":"10.3390/vetsci12020148","DOIUrl":"10.3390/vetsci12020148","url":null,"abstract":"<p><p>The use of agro-industrial by-products in livestock nutrition is of increased interest to the feed industry. Some of the by-products are rich in bioactive compounds that could lead to an increase in the quality of animal meat products. This study aimed to evaluate the effects of dietary incorporation of grape pomace (GP), a by-product rich in polyphenols, on growth and on the oxidation status and the fatty acid profile of rabbit meat. Forty-eight male rabbits (<i>Oryctolagus cuniculus</i>) (aged 55 ± 3 d and ca 2.3 kg) divided into three groups of 16 were used in the trial. The rabbits were housed in pairs in wire-mesh cages (26 × 46 × 35 cm) giving n = 8 for each diet treatment. The first group was fed a basal diet (CTR) (ca 18% protein and ca 4% fat), the second was given a basal diet supplemented with 5 g GP per head per day (low-GP) and the third group was fed a basal diet supplemented with 10 g GP per head per day (high-GP). The trial lasted three weeks, and the animals were weighed weekly. The rabbits were slaughtered, and the weight and the pH of the carcasses was measured immediately after slaughter and after 24 h of cooling at 4 °C. The pH of the stomach and cecum was measured. A sample of <i>Longissimus Dorsi</i> (LD) was collected and stored at -80 °C for the analysis of proximate composition, thiobarbituric acid reactive substances (TBARs) and fatty acid profile (FA). The diet did not influence the growth or the pH of the stomach and cecum of the rabbits. The chemical composition of the meat was similar in all groups, with a mean protein of 22.3% and fat of 0.95%. The fatty acid profiles of the LD were not influenced by the diet, and the most abundant FAs were C16:0, C18:2n6 and C18:1c9. Feeding GP to the rabbits gave a linear decrease of TBARs in the meat, indicating a reduction in oxidative stress. The TBARs' reduction was 60% in the meat of the rabbits given high-GP compared to CTR. In conclusion, GP could be used in rabbit diets without any negative influence on meat quality but with an increase in oxidation stability that could enhance meat shelf-life.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11860478/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Peng Li, Isadora Machado, Thomas Petznick, Emily Pratt, Jinnan Xiao, Chris Sievers, Paul Yeske, Swami Jayaraman, Daniel C A Moraes, Guilherme Cezar, Mafalda Mil-Homens, Hao Tong, Kelly Will, Darwin Reicks, Jason Kelly, Onyekachukwu H Osemeke, Gustavo S Silva, Daniel C L Linhares
{"title":"PRRSV RNA Detection and Predictive Values Between Different Sow and Neonatal Litter Sample Types.","authors":"Peng Li, Isadora Machado, Thomas Petznick, Emily Pratt, Jinnan Xiao, Chris Sievers, Paul Yeske, Swami Jayaraman, Daniel C A Moraes, Guilherme Cezar, Mafalda Mil-Homens, Hao Tong, Kelly Will, Darwin Reicks, Jason Kelly, Onyekachukwu H Osemeke, Gustavo S Silva, Daniel C L Linhares","doi":"10.3390/vetsci12020150","DOIUrl":"10.3390/vetsci12020150","url":null,"abstract":"<p><p>Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), a major challenge for the swine industry, can be transmitted both vertically and horizontally. Common sample types, including processing fluid, serum, and family oral fluid, can neither determine whether PRRSV infection originates vertically or horizontally nor directly reflects the sow's PRRSV status. At around 90 days post live-virus inoculation (LVI), 555 sows were sampled by Tonsil-oral-scrubbing (TOSc) 2 weeks pre-farrowing and tested for PRRSV RNA. From these, 59 PRRSV-positive sows, and 88 PRRSV-negative sows matched by parity were conveniently selected. TOSc from sows, blood swabs from live piglet, and tongue fluid (TF) plus serum from dead piglet were collected individually from all study litters within 12 h post-farrowing. The pre-farrowing TOSc samples had significantly higher PRRSV positivity than TF, serum and blood swab pools, while dead piglet serum had significantly lower mean Ct values than all other sample types. TOSc samples had 25% positive predictive value (PPV) for \"live litter\" PRRSV status while the pre-farrow and post-farrow TOSc had 87.2% and 89.0% negative predictive value (NPV), respectively. In conclusion, we characterized PRRSV RNA detection among all sub-populations within a litter with easy-to-use TOSc samples and neonatal litter samples, suggesting the occurrence of vertical transmission 90 days post-LVI in sows. TOSc samples from sows had low PPV and high NPV for their respective litter's PRRSV status.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11861758/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Areej Arif, Nousheen Zahoor, Jianqiang Tang, Meihui Tang, Liyue Dong, Sardar Zarq Khan, Guojun Dai
{"title":"Cryopreservation Strategies for Poultry Semen: A Comprehensive Review of Techniques and Applications.","authors":"Areej Arif, Nousheen Zahoor, Jianqiang Tang, Meihui Tang, Liyue Dong, Sardar Zarq Khan, Guojun Dai","doi":"10.3390/vetsci12020145","DOIUrl":"10.3390/vetsci12020145","url":null,"abstract":"<p><p>Poultry semen preservation is crucial for sustaining genetic diversity, improving production efficiency, and facilitating various breeding initiatives. This review underscores the importance and challenges associated with different preservation techniques. We investigate effective techniques for semen collection and evaluation, focusing on vital parameters such as volume, concentration, motility, and morphology. Preservation strategies are categorized into short-term approaches, such as dilution with extenders, and mid-to-long-term strategies, like freezing and the use of cryoprotectants. Additionally, we explore several factors affecting semen quality, including male age and genetics, seasonal impacts, and stress during handling. Assessing the quality of preserved semen is critical, particularly regarding post-thaw motility. The applications of these preservation techniques in artificial insemination, genetic enhancement, the conservation of endangered breeds, and biobanking are highlighted. This review identifies critical research opportunities, including the development of improved cryoprotectants, refining freezing protocols, comprehending the mechanisms of semen damage, and innovating novel preservation technologies. Addressing these challenges will enhance poultry semen preservation and contribute to sustainable poultry farming.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11861739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sze-Min Chan, Ching-Li Tseng, Wei-Hsiang Huang, Chung-Tien Lin
{"title":"Therapeutic Assessment of Diverse Doxycycline-Based Formulations in Promoting Deep Corneal Wound Healing: Evidence from a Rat Model.","authors":"Sze-Min Chan, Ching-Li Tseng, Wei-Hsiang Huang, Chung-Tien Lin","doi":"10.3390/vetsci12020143","DOIUrl":"10.3390/vetsci12020143","url":null,"abstract":"<p><p>Doxycycline (Dxy), a broad-spectrum antibiotic with anti-inflammatory effects, is commonly used in ophthalmology but is unstable as a topical eyedrop, degrading quickly into inactive forms and requiring frequent application. To address this, gelatin nanoparticles (GNPs) loaded with Dxy (DNPs) were developed as a stable ophthalmic nanomedicine for enhancing corneal wound healing by inhibiting matrix metalloproteinases (MMPs). In this study, female Sprague-Dawley rats underwent lamellar keratectomy, and various Dxy formulations-oral, conventional eyedrops, and DNP-containing eyedrops-were evaluated for corneal wound repair. Clinical assessments included fluorescein staining, slit-lamp biomicroscopy, spectral-domain optical coherence tomography (SD-OCT) imaging, histopathology, and immunohistochemistry for MMP-2, MMP-9, and α-SMA. The DNP group (0.01% Dxy in DNPs, applied twice daily) demonstrated faster corneal thickness recovery and epithelial healing on days 7 and 14 compared to 0.1% Dxy eyedrop treatments applied twice or four times daily. DNP-treated eyes also showed reduced angiogenesis intensity and lower MMP-2 and MMP-9 immunoreactive scores, with enhanced stromal recovery and reduced neovascularization. These results highlight DNPs' potential as a superior treatment for corneal wounds, providing effective healing with less frequent dosing and lower drug concentrations. This study supports DNPs' potential for clinical application as a stable and efficient therapeutic agent in ophthalmology.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11860526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eduardo Valdez-Romero, Lucía Delgadillo-Ruiz, Rómulo Bañuelos-Valenzuela, Eladio Delgadillo-Ruiz, Carlos Meza-López, Marisa Mercado-Reyes, Leticia Adriana Ramírez-Hernández, Francisco G Echavarría-Chairez, Luz Adriana Arias-Hernández, Benjamín Valladares-Carranza, Rodrigo Flores-Garivay, Héctor Emmanuel Valtierra-Marín
{"title":"Bromatological Composition and In Vitro Ruminal Digestibility of Vaines of <i>Neltuma</i> spp. in Three Regions of the State of Zacatecas, México.","authors":"Eduardo Valdez-Romero, Lucía Delgadillo-Ruiz, Rómulo Bañuelos-Valenzuela, Eladio Delgadillo-Ruiz, Carlos Meza-López, Marisa Mercado-Reyes, Leticia Adriana Ramírez-Hernández, Francisco G Echavarría-Chairez, Luz Adriana Arias-Hernández, Benjamín Valladares-Carranza, Rodrigo Flores-Garivay, Héctor Emmanuel Valtierra-Marín","doi":"10.3390/vetsci12020142","DOIUrl":"10.3390/vetsci12020142","url":null,"abstract":"<p><p><i>Neltuma</i> spp. are an important resource in arid and semiarid ecosystems of the country and serve, among other purposes, as an economic source of food for livestock. By analyzing the bromatological composition and in vitro fermentation of pods in three regions of the state of Zacatecas, Mexico, the viability as a food alternative in sheep is sought. Twenty-seven pod samples were collected from nine points in each region of the state. The samples were obtained when the pods were in the immature stage. The bromatological analysis of the pods was conducted and mathematical equations were used to calculate the megacalories per kilogram provided by these fruits. Statistical analysis was performed using Spearman's correlation coefficient. Bromatological analysis showed that crude protein averaged 20.86 ± 1.37% in the central region, while non-fiber carbohydrates averaged 26.78 ± 1.32% in the southern region. <i>Neltuma</i> spp. pods demonstrate potential as an alternative feed for sheep, provided that an adequate diet is formulated, since they present acceptable levels of protein, carbohydrates and fiber. It is recommended to consider the state of maturity of the pods when incorporating them into the diet of sheep.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11860705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"STEAP3 Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication by Regulating Fatty Acid and Lipid Droplet Synthesis.","authors":"Chenyang Yuan, Kaifeng Guan, Gaiping Zhang","doi":"10.3390/vetsci12020147","DOIUrl":"10.3390/vetsci12020147","url":null,"abstract":"<p><p>Porcine Reproductive and Respiratory Syndrome (PRRS) is a contagious disease that impacts swine health worldwide. Lipid metabolism plays a vital role in energy production and is regulated by various genes involved in lipogenesis and lipolysis. In this study, we found that PRRSV infection significantly reduced the protein expression of STEAP3. The overexpression of STEAP3 can notably inhibit PRRSV replication. Additionally, we utilized transcriptomics and metabolomics to examine the effects of STEAP3 on PRRSV replication, identifying important pathways associated with energy metabolism and lipogenesis. We subsequently found that STEAP3 can suppress PRRSV replication by regulating fatty acid synthesis and enhancing lipid droplet formation. Overall, these findings indicate that STEAP3 could be a potential target for developing strategies to manage PRRSV infection by modulating lipid metabolism.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11861627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Prevalence of Tick-Borne Encephalitis Virus in the Ticks and Humans of China from 2000 to 2023: A Systematic Review and Meta-Analysis.","authors":"Hongyu Qin, Xiu Xin, Qichao Tang, Xujing Feng, Baishuang Yin","doi":"10.3390/vetsci12020146","DOIUrl":"10.3390/vetsci12020146","url":null,"abstract":"<p><p>This study presents a systematic review and meta-analysis of studies on tick-borne encephalitis virus (TBEV) prevalence in ticks and human hosts in China, published between 2000 and 2023. Extensive searches were conducted on four databases-PubMed, CNKI, VIP, and Wan Fang. The findings indicated overall pooled prevalence estimates of TBEV infection in ticks and humans of 5.8% and 9.0%, respectively. The prevalence of TBEV in ticks was 4.8% (95%CI, 4.5-7.1%) during 2000-2010 and increased to 6.3% (95%CI, 4.7-7.8%) during 2011-2023, and was mainly distributed in Jilin (13.4%; 95%CI, 8.3-18.4%) and Inner Mongolia (4.5%; 95%CI, 1.8-7.1%). The prevalence of TBEV was higher in <i>Dermacentor silvarum</i> than in other tick species (8.1%; 95%CI, 3.2-12.9%). The seroprevalence of TBEV in humans increased from 4.7% (95%CI, 2.9-6.4%) during 2000-2010 to 17.6% (95%CI, 11.3-23.8%) during 2011-2023. Simultaneously, the analysis results of the population characteristics showed that females, forestry workers, military personnel, and farmers were found to be susceptible, and the highest seroprevalence was noted among homemakers and unemployed individuals. There were certain differences in the seroprevalence among populations in different provinces, especially in Xinjiang and Heilongjiang. This study can provide a reference for a more comprehensive and in-depth investigation of ticks and humans infected with TBEV in China.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11861706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jemberu Alemu Megenas, Mengistu Legesse Dadi, Tesfu Kassa Mekonnen, James W Larrick, Gezahegne Mamo Kassa
{"title":"Seroprevalence of West Nile Fever and Associated Risk Factors in Livestock of Afar Region, Northeast Ethiopia.","authors":"Jemberu Alemu Megenas, Mengistu Legesse Dadi, Tesfu Kassa Mekonnen, James W Larrick, Gezahegne Mamo Kassa","doi":"10.3390/vetsci12020141","DOIUrl":"10.3390/vetsci12020141","url":null,"abstract":"<p><p>Sub-Saharan Africa has long been prone to widespread mosquito-borne diseases affecting both humans and animals. However, the presence and impact of West Nile virus (WNV) among livestock in Ethiopia have not been thoroughly investigated. The objective of this study was to investigate the seroprevalence of West Nile virus in livestock in the Afar region using serological methods. A total of 736 serum samples were collected from 224 cattle, 155 camels, 144 sheep, 121 goats, and 92 donkeys in the Amibara and Haruka districts of the Afar region selected using haphazard sampling. Among 736 tested livestock serum samples, 50.7% (373/736) showed anti-WNV IgG antibodies evaluated using the ID screen<sup>®</sup> WNV competition multispecies ELISA kits (95% CI: 47-54.4%; <i>p</i> < 0.01). The seroprevalence was higher (<i>p</i> < 0.01) in donkeys (76.1%), followed by camels (69.1%), cattle (52.2%), goats (34.7%), and sheep (25.7%). The study showed a statistically significant difference of WNV seropositivity between species of animals AOR (1.5), 95% CI (1.038-2.212) (<i>p</i> < 0.01). Compared with sheep, donkeys had a seven-fold higher chance of being seropositive for WNV infection (OR: 6.447, 95% CI = 3.888-10.688) (<i>p</i> < 0.01). This study emphasizes how common WNV infection is in Ethiopia's pastoral Afar region. It is imperative to consider consistent surveillance of WNV infection and prompt management of identified WNV disease in clinical practice. A clear need exists to build additional research capacity regarding WNV infections among both humans and animals.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11860265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Assessment of Resistance of <i>Varroa destructor</i> to Formic and Lactic Acid Treatment-A Systematic Review.","authors":"Yvonne Kosch, Christoph Mülling, Ilka U Emmerich","doi":"10.3390/vetsci12020144","DOIUrl":"10.3390/vetsci12020144","url":null,"abstract":"<p><p><i>Varroa destructor</i> represents one of the most significant pathogens affecting <i>Apis mellifera</i>. A multitude of treatment modalities exist, including those based on pharmaceutical and biotechnological principles. A few years after the first use of synthetic acaricides, <i>Varroa destructor</i> developed resistance to these substances, becoming a significant concern. The present review assesses the potential risk of resistance development to formic and lactic acid. This is performed by evaluating sources in the literature over the past 30 to 40 years following the PRISMA 2020 guidelines. Median annual efficacies are determined and evaluated over time. Independent of pharmaceutical form, mite populations are considered non-resistant for efficacies higher than 70%. After the assessment of outliers for lactic acid, the remaining limited data are insufficient to draw conclusions on resistance. The reported unusually low efficacy values for formic acid can be explained by inadequate evaporation rates and study settings. Notably, for both acids, the findings are limited by a low number of efficacy values, and further, more detailed and standardized studies are needed.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11860394/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143503803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rosalie Fortin-Choquette, Jason B Coe, Cathy A Bauman, Lori M Teller
{"title":"Promoters and Detractors Identify Virtual Care as \"Worlds Better than Nothing\": A Qualitative Study of Participating Veterinarians' Perception of Virtual Care as a Tool for Providing Access.","authors":"Rosalie Fortin-Choquette, Jason B Coe, Cathy A Bauman, Lori M Teller","doi":"10.3390/vetsci12020136","DOIUrl":"10.3390/vetsci12020136","url":null,"abstract":"<p><p>(1) Background: Veterinary virtual care holds the potential to alleviate some barriers to accessing care, yet concerns within the profession exist. Understanding veterinarians' perspectives and identifying the potential opportunities and challenges that virtual care poses for access to veterinary care are thus needed. (2) Methods: Semi-structured interviews were conducted virtually with 22 companion-animal veterinarians practicing across Canada and the United States. Interviews were accompanied by an electronic survey, with which a Net Promoter Score (NPS) was calculated for each participant. Using their NPS, participants were categorized as a \"promoter\" or \"detractor\", with respect to their perspective on veterinary virtual care. A thematic analysis was conducted on verbatim transcripts of the interviews. (3) Results: A total of 11 detractors and 11 promoters were interviewed. Four subthemes were identified, including the following: (1) there are limitations to virtual care, (2) virtual care plays a role in access to care, (3) \"virtual care is better than no care\" and (4) virtual care offers specific value in supplementing in-person care. (4) Conclusion: When no other option for care delivery exists, virtual care was viewed as a way to increase access to veterinary care.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"12 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11860569/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}