Veterinary Sciences最新文献

{"title":"Prognostic Value of Clinicopathological Parameters Assessed During Admission of Foals with Neonatal Encephalopathy and Comorbidities Developed During Hospitalization.","authors":"Alexandra Vilela E Maia, José Pimenta, Mário Cotovio","doi":"10.3390/vetsci11110534","DOIUrl":"10.3390/vetsci11110534","url":null,"abstract":"<p><p>Neonatal encephalopathy represents a broad neurological syndrome which encompasses newborn foals presenting a variety of non-infectious neurologic signs and/or abnormal behaviors in the immediate postpartum period. It is recognized as the most predominant neurological disorder in neonatal foals. Prognostic factors can guide clinicians in medical decision-making, aiding in the establishment of survival probabilities. The aim of this work was to scrutinize the prognostic value of clinical and laboratorial findings upon admission and posterior comorbidities developed during hospitalization, assessing their influence on the outcome of foals with NE. For this purpose, the medical records of 61 neonatal foals with a primary diagnosis of NE were retrospectively analyzed. The overall survival rate was 57.4%. Most foals presented recumbency at admission, which was associated with higher mortality rates (<i>p</i> = 0.002). Hypothermic foals at admission were 4.85 times more likely to succumb (<i>p</i> = 0.015). The presence of hypoglycemia at admission was associated with higher mortality rates (<i>p</i> = 0.002). Foals with hypercreatinemia at admission had 6.67 times greater odds of dying. The development of seizures contributed to 4.14 greater odds of dying. Foals that developed comorbidities during hospitalization had 40.1 times greater odds of dying, with pneumonia and sepsis being the most relevant comorbidities. In foals with NE, rectal temperature, hematocrit, blood glucose, and creatinine concentrations are simple, quick-to-measure parameters that may have prognostic value during admission.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598894/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of the Fire Hose Square Knot Browser as a Foraging Enrichment Device on the Behavior of Captive <i>Macaca fascicularis</i>.","authors":"Puji Rianti, Tamara M Anisa, Huda S Darusman","doi":"10.3390/vetsci11110535","DOIUrl":"10.3390/vetsci11110535","url":null,"abstract":"<p><p>Effective management of captive <i>M. fascicularis</i> (long-tailed macaques) is crucial for maintaining high-quality research models, necessitating strategies to promote their welfare. This study evaluated the impact of a foraging enrichment device, the \"fire hose square knot browser\", on the behavior of 32 long-tailed macaques at the Primate Research Center of IPB University in Bogor, Indonesia. We observed and analyzed daily behaviors across various food types over 288 h using scan and instantaneous sampling methods. Statistical analyses, including ANOVA and Kruskal-Wallis tests, revealed significant behavioral changes in the presence of the foraging enrichment device. The foraging enrichment device notably increased eating and affiliative behaviors, while reducing resting, agonistic, and stereotypic behaviors. No significant differences in eating, auto-grooming, resting, sexual, agonistic, or stereotypic behaviors were observed between the morning and afternoon. However, mobility and affiliation behaviors varied between these periods. The highest percentage of eating behavior occurred with the enrichment foraging device and a mixture of fruits. Furthermore, a three-way non-parametric analysis suggests a significant effect of food type and treatment on behaviors such as mobility and stereotyping. These findings underscore the positive impact of foraging enrichment devices on promoting active behavior and enhancing animal welfare in captive long-tailed macaques.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole Blood Transcriptome Analysis in Dairy Ewes Fed a Dietary Grape Pomace Supplementation.","authors":"Andrea Ianni, Francesca Bennato, Camillo Martino, Maria Antonietta Saletti, Francesco Pomilio, Giuseppe Martino","doi":"10.3390/vetsci11110536","DOIUrl":"10.3390/vetsci11110536","url":null,"abstract":"<p><p>The present study aims to evaluate the effect of a dietary supplementation with 10% grape pomace (GP) on the whole blood transcriptome of lactating ewes. By applying a log₂FC higher than 0.5 or lower than -0.5 and a false discovery rate (FDR) <0.05, the down-regulation of genes coding for plexin C1, ethanolamine kinase 1, tax1-binding protein 1, transmembrane 9 superfamily member 2, and Beclin-1 was observed in animals that received the dietary supplementation. This aspect was also accompanied by a reduction in the blood activity of matrix metalloproteinase 9 (MMP-9; <i>p</i> < 0.05), a gelatinase commonly involved in both acute and chronic pathological events. The ELISA test on other factors involved in inflammatory processes, interleukin 1 (IL-1) and tumor necrosis factor α (TNF-α), as well as in the antioxidant response, glutathione peroxidase (GPx), and catalase (CAT), did not reveal any significant changes (<i>p</i> > 0.05). Overall, the introduction of GP in the diet of ewes gave indications of greater efficacy in preserving animal welfare, with interesting cues regarding the valorization of a by-product with a high biological value.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11599069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Social Housing of Previously Single-Caged Adult Male Cynomolgus Macaques (<i>Macaca fascicularis</i>).","authors":"Fitriya N Dewi, Permanawati, Suryo Saputro, Adinda D Azhar, Wahyu Putriyani, Jeffrey D Wyatt, Irma H Suparto, Huda S Darusman","doi":"10.3390/vetsci11110538","DOIUrl":"10.3390/vetsci11110538","url":null,"abstract":"<p><p>Cynomolgus macaques (<i>Macaca fascicularis; Mf</i>) serve an essential role in the advancement of biomedical research. Aged macaques, in particular, are highly valued as animal models for the study of geriatric diseases. While social housing has become the default for nonhuman primates (NHPs), socially housing sexually mature males poses a unique challenge. Moreover, socially housing aged males that have been previously kept in single housing may pose even greater challenge and risk due to a higher likelihood of aggression. Temperament assessment plays an integral part in determining the success of social housing arrangements of NHPs. In this paper, we report our work at the Primate Research Center of IPB University in integrating behavior observation and veterinary management to socially house adult, male <i>Mf.</i> We describe our experience in successfully housing young-adult, male <i>Mf</i> (<i>n =</i> 8-10, aged 6 years) for over 2.5 years, and the multiple efforts to socially house a small group of previously singly housed aged, male <i>Mf</i> (<i>n</i> = 6, aged >15 years). The temperament of each <i>Mf</i> was considered in the planning and implementation of social housing attempts. While a simplified behavioral observation was performed for the young adults, a more rigorous PAIR-T assessment was performed on the aged <i>Mf</i>. A group formation was initially attempted, aiming to achieve a small group of six aged, male <i>Mf</i>. While this group at first showed promising outcomes, significant incidents of aggression required regrouping as pairs or triads wherein the subject combinations were determined based on temperament and dominance. A total of three attempts were made to house these aged <i>Mf</i> in a small group throughout the course of 17 months, and the results showed that at our facility, aged, male <i>Mf</i> are best housed as triads or pairs, with selections based on their temperament and dominance compatibility. We concluded that behavioral assessments and veterinary management are pivotal in supporting the social housing efforts of adult, male <i>Mf</i> in order to optimize their well-being.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11599010/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment with Leflunomide in Conjunction with Glucocorticoids for Dogs with Immune-Mediated Polyarthritis Is Not Associated with Improved Outcomes: A Retrospective Cohort Study of 93 Dogs from Australia (2017-2024).","authors":"Remon Wilson, Inar Swift, Mikaela Groth-Semple, Sabrina Lee, Tamara Dann, Ahmed Arafa, Curtis Poyton, Mary Thompson","doi":"10.3390/vetsci11110537","DOIUrl":"10.3390/vetsci11110537","url":null,"abstract":"<p><p>Immune-mediated polyarthritis (IMPA) has a relatively high relapse rate compared to other immune-mediated diseases. Leflunomide is frequently used to treat dogs with IMPA in conjunction with prednisolone. This retrospective cohort study aimed to evaluate the therapeutic efficacy of leflunomide as an adjunctive therapy to prednisolone in reducing relapse and mortality rates in dogs diagnosed with IMPA in Australia. The medical records of client-owned dogs diagnosed with IMPA at a specialist referral hospital in Southeast Queensland from 2017 to 2024 were reviewed. A total of 93 dogs were included in this study, divided into two groups based on the treatment received: Group PRED, consisting of 53 dogs treated with prednisolone as the sole immunosuppressive agent, and Group L+PRED, consisting of 40 dogs that received leflunomide as adjunctive therapy alongside prednisolone. Data collected included breed, age, weight, sex, serum C-reactive protein concentration, results of synovial fluid analysis and microbial culture, treatment protocol, relapse rates and time to relapse, and mortality rates. There was no difference in relapse or mortality rates, time to relapse, nor time to discontinue prednisolone between the PRED and L+PRED groups. The L+PRED group had higher body weights and lower prednisolone dose rate at discharge compared to those in the PRED group. This study demonstrated that the use of leflunomide as an adjunctive therapy to prednisolone for the treatment of dogs with IMPA had no improved outcomes, reduced relapse rates, or shortening in the duration of prednisolone therapy when compared to dogs receiving prednisolone monotherapy.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11599048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Evaluation of a Novel Chimeric Genotype VII Newcastle Disease Vaccine: Overcoming Maternal Antibody Interference and Spray Administration.","authors":"Xiaoquan Wang, Yao Yao, Wenhao Yang, Xiaolong Lu, Ruyi Gao, Kaituo Liu, Yu Chen, Min Gu, Jiao Hu, Shunlin Hu, Xiufan Liu, Xiaowen Liu","doi":"10.3390/vetsci11110532","DOIUrl":"10.3390/vetsci11110532","url":null,"abstract":"<p><p>Newcastle disease virus (NDV) poses a significant threat to the poultry industry, with the emergence of genotype VII NDV leading to extensive outbreaks and economic losses. Vaccination is the primary means of controlling NDV, but the presence of maternal antibodies (MDAs) can interfere with the immunological effect of live virus vaccines. Thus, we constructed a chimeric NDV live virus vaccine, LX-OAI4S, by replacing the extracellular regions of the F and HN genes of the NDV LX strain with the corresponding regions of the A-VII vaccine strain. The chimeric vaccine LX-OAI4S demonstrated high genetic stability, good safety, and strong reproductive capacity in chicken embryos. The LX-OAI4S vaccine induced rapid antibody production in specific pathogen-free (SPF) and commercial chickens via the intranasal and intraocular (IN/IO) routes, with hemagglutination inhibition (HI) antibody titers reaching 4.71 ± 1.03 log₂ at 7 days post-vaccination (dpv), significantly higher than those of the two classical vaccine strains La Sota and VG/GA. The LX-OAI4S vaccine group provided effective protection against the challenge of genotype VII NDV virulent strain JS2/06 and inhibited viral shedding. When administered via spray, the LX-OAI4S vaccine elicited high systemic immunity against NDV in both SPF and commercial chickens, effectively protecting against clinical disease and reducing viral shedding. The chickens were exposed to high-dose vaccine for spray vaccination, and no adverse reactions were observed after vaccination. Despite the presence of anti-NDV MDAs in chickens, the NDV-specific antibody titers were significantly greater in the vaccinated groups than in the unvaccinated group. The vaccine exhibited high immunogenicity and the potential to overcome maternal antibody interference. The LX-OAI4S vaccine is a promising candidate for an ND vaccine. Its administration via spray can effectively prevent the occurrence of ND, making it a valuable tool for the poultry industry.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11599132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feline Hemotropic <i>Mycoplasma</i> Species of Apparently Healthy Domestic Cats in Konya Province of Türkiye.","authors":"Ceylan Ceylan, Muhammed Hudai Culha, Gonca Sonmez, Muhammed Ahmed Selcuk, Merve Ider, Ayşe Evci, Sule Yılmaz, Ferda Sevinc, Onur Ceylan","doi":"10.3390/vetsci11110530","DOIUrl":"10.3390/vetsci11110530","url":null,"abstract":"<p><p>Feline hemotropic mycoplasmosis is an emerging infectious disease in domestic cats caused by a group of hemotropic <i>Mycoplasma</i> species, including three main species: <i>Mycoplasma hemofelis</i> (<i>Mhf</i>), <i>Candidatus</i> Mycoplasma hemominutum (<i>C</i>Mhm), and <i>Candidatus</i> Mycoplasma turicensis (<i>C</i>Mt). The primary objective of this investigation was to ascertain the molecular prevalence and characterization of hemotropic mycoplasmas in domestic cats from twenty-three districts of Konya province in Türkiye. For this purpose, 384 apparently healthy cats belonging to different breeds were enrolled in this study. The molecular prevalence of <i>Mycoplasma</i> spp. in cats was 9.4%, and the identified species included <i>Mhf</i>, <i>Mycoplasma hemocanis</i> (<i>Mhc</i>), <i>C</i>Mt, and <i>C</i>Mhm. The molecular prevalence was not significantly associated with district, breed, or gender (<i>p</i> > 0.05) but was significantly associated with age and ownership status (<i>p</i> < 0.05). The study found that all cats with <i>Mycoplasma</i> spp. were older than one year and that the infection had a higher prevalence in stray cats than in owned cats (<i>p</i> < 0.05). Although there are studies conducted in different provinces, data on the molecular and phylogenetic characterization of the species causing feline hemotropic mycoplasmosis are scarce in Türkiye. This study, which provides updated data concerning the molecular characterization and phylogeny of hemotropic <i>Mycoplasma</i> species of cats in Türkiye, revealed the <i>Mycoplasma</i> spp. molecular prevalence in Konya province for the first time and provided remarkable findings that will fill the scientific gap in the country. The molecular characterization of these hemotropic mycoplasmas is crucial for understanding their epidemiology and developing effective diagnostic and treatment strategies for feline hemotropic mycoplasmosis. It is anticipated that the data from the study will raise awareness among pet owners, veterinarians, and healthcare professionals of feline hemotropic <i>Mycoplasma</i> agents with zoonotic potential.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11599055/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction Network Characterization of Infectious Bronchitis Virus Nsp2 with Host Proteins.","authors":"Mengmeng Wang, Zongyi Bo, Chengcheng Zhang, Mengjiao Guo, Yantao Wu, Xiaorong Zhang","doi":"10.3390/vetsci11110531","DOIUrl":"10.3390/vetsci11110531","url":null,"abstract":"<p><p>Infectious bronchitis (IB) is a highly contagious acute viral disease that leads to substantial economic losses in the poultry industry. Previous research conducted in our laboratory has indicated that Nsp2 may serve as a key virulence factor within the IBV genome, as evidenced by its pronounced divergence between the field strain and its attenuated counterpart. Understanding the interaction between Nsp2 and host proteins is crucial to elucidating the role of the Nsp2 protein in the pathogenesis and proliferation of IBV. Currently, much remains to be uncovered regarding the host proteins that interact with the IBV Nsp2 protein. In this study, 10 host proteins, including COX1, COX3, NFIA, ITGA1, ATP1B1, ATP1B3, ABCB1, ISCA1, DNAJA1, and IREB2, were screened to interact with IBV Nsp2 through yeast two-hybrid experiments and molecular docking simulations. Furthermore, the interaction of Nsp2 with ATP1B3, DNAJA1, and ISCA1 proteins was further validated through co-immunoprecipitation and confocal experiments. The GO, KEGG, and PPI databases revealed that the host proteins interacting with Nsp2 are primarily associated with ATPase activation, Fe-S cluster binding, ion homeostasis, and innate immune regulation. The examination of the expression levels of these Nsp2-interacting host proteins during IBV infection demonstrated the significant downregulation of COX3, COX1, ATP1B1, and ATP1B3, while NFIA, DNAJA1, and IREB2 showed significant upregulation. Moreover, our study identified that IBV enhances viral replication by upregulating DNAJA1 expression, although the underlying mechanism requires further investigation. These findings provide valuable insights into the potential role of the Nsp2 protein in the pathogenesis of IBV.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142732317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Empowering Veterinary Herd Health Management: Insights into Education, Implementation, and Regulation Across Europe.","authors":"Marina Marić, Vidhi Manghnani, Jarkko K Niemi, Tarmo Niine, Nancy De Briyne, Wiebke Jansen","doi":"10.3390/vetsci11110528","DOIUrl":"10.3390/vetsci11110528","url":null,"abstract":"<p><p>In recent years, integrated veterinary herd health management (VHHM), improving animal health, welfare, food safety, public health, farm management, and economics, has become ever-more important in the European Union due to structural and regulatory advancements. A mixed-methods study analyzed the current state and gaps in VHHM education and training across Europe. Data from 41 European veterinary institutions showed that 83% (<i>n</i> = 34/41) offered VHHM education, either as standalone courses and/or integrated into other subjects. An online survey for veterinarians, primarily working with cattle, poultry, pigs, and small ruminants (75%; <i>n</i> = 86/114), assessed perceived training knowledge gaps and needs. The majority of respondents were unaware of VHHM training programs (69.4%, <i>n</i> = 43/62) or projects (59.6%, <i>n</i> = 59/99) and reported the biggest gaps in VHHM training certification (14%, <i>n</i> = 7/49), followed by soft skills (12%, <i>n</i> = 6/52) and demonstrating VHHM benefits to their clients (9%, <i>n</i> = 5/53). To conclude, while undergraduate VHHM education was generally adequate, enhancing post-graduate certified multi-species training opportunities, incl. soft skills VHHM training were perceived as essential. Our findings also underscored the urgent need for robust economic evaluations of preventive strategies across various animal species to allow veterinarians to better demonstrate VHHM's direct and indirect benefits to farmers.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of the First Case of Dedifferentiated Liposarcoma in Amphibians: Insights from <i>Maculopaa medogensis</i>.","authors":"Runliang Zhai, Tianyu Qian, Yonghong Wang, Bin Wang, Cheng Li, Feng Xie, Wei Zhu, Jianping Jiang","doi":"10.3390/vetsci11110526","DOIUrl":"10.3390/vetsci11110526","url":null,"abstract":"<p><p>Dedifferentiated liposarcoma (DDLPS) is a rare and aggressive type of cancer primarily reported in humans, with no documented cases in animals. In this study, we present the first case of DDLPS in a wild amphibian species, <i>Maculopaa medogensis</i>. The tumor was discovered during a routine examination and diagnosed through a combination of advanced diagnostic methods, including micro-CT imaging, gross anatomical inspection, histological analysis, and immunohistochemistry. The tumor exhibited both well-differentiated and dedifferentiated components, characteristic of DDLPS, with evidence of tissue invasion and multiple metastases. Immunohistochemical analysis revealed the strong positive expression of markers such as S100A4, CDK4, MDM2, and CD34, while Leptin expression was negative, further confirming the diagnosis. This is the first reported case of DDLPS in a non-human species, expanding our understanding of cancer in wildlife and underscoring the significance of amphibians as environmental indicators. These findings provide valuable insights for veterinary medicine and wildlife conservation, particularly regarding the role of environmental stressors in cancer development. This study highlights the need for comprehensive diagnostic approaches in wildlife pathology.</p>","PeriodicalId":23694,"journal":{"name":"Veterinary Sciences","volume":"11 11","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11598943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142732300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}