2023 - 2025年南疆猪繁殖与呼吸综合征病毒分子流行病学及遗传进化分析

IF 2.3 2区 农林科学 Q2 VETERINARY SCIENCES
Shuhua Liu, Mengzhe Hou, Xin Chen, Baihe Ma, Zhen Zhang, Meiliang Guo, Yunlai Chen, Lianrui Li
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引用次数: 0

摘要

猪繁殖与呼吸综合征病毒(PRRSV)是造成全球养猪业重大经济损失的主要病原体,本文对其在新疆南部地区的流行特征进行了研究。对2023 - 2025年从13个猪场采集的632份临床标本进行RT-PCR检测,总体阳性率为18.35%(116/632),其中PRRSV-2单一感染占97.41% (113/116),PRRSV-1单一感染占1.72%(2/116),合并感染占0.86%(1/116)。在阳性样品获得的38条ORF5序列中,亚谱系1.8 (nadc30样)占优势,占成功测序菌株的97.14%。分子分析显示,PRRSV-1分离株携带6个氨基酸突变,包括A129V(与中国毒株CN/FJFQ-1/2023一致),而PRRSV-2分离株具有中和逃逸突变Q13R、毒力相关位点K151R和异常疫苗标记A137等关键突变。此外,在NADC30和VR-2332之间鉴定出重组菌株XJLETUQ2025-7,其断点位于NSP2和NSP10。血清学监测显示,2043头接种猪的总抗体阳性率为83.0%(1696/2043),年阳性率从72.3%(2023)和75.4%(2024)上升到91.3%(2025)。综上所述,南疆PRRSV流行主要由PRRSV-2 nadc30样毒株驱动,重组事件和GP5表位变异给疾病控制带来挑战。这些发现增强了对该地区PRRSV流行病学的认识,并为疫苗开发和预防策略提供了有价值的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular Epidemiology and Genetic Evolution Analysis of Porcine Reproductive and Respiratory Syndrome Virus in Southern Xinjiang, China, from 2023 to 2025.

Porcine reproductive and respiratory syndrome virus (PRRSV), a major pathogen causing substantial economic losses in the global swine industry, was studied in southern Xinjiang to characterize its local epidemic features. Based on 632 clinical samples collected from 13 pig farms between 2023 and 2025, quantitative RT-PCR detection showed an overall positivity rate of 18.35% (116/632), with PRRSV-2 single infection accounting for 97.41% (113/116), PRRSV-1 single infection for 1.72% (2/116), and co-infection for 0.86% (1/116). Among 38 ORF5 sequences obtained from positive samples, Sublineage 1.8 (NADC30-like) was dominant, comprising 97.14% of successfully sequenced strains. Molecular analysis revealed that PRRSV-1 isolates carried six amino acid mutations including A129V (consistent with the Chinese strain CN/FJFQ-1/2023), while PRRSV-2 strains exhibited key variations such as the neutralization escape mutation Q13R, virulence-associated site K151R, and an anomalous vaccine marker A137. Furthermore, a recombinant strain (XJLETUQ2025-7) between NADC30 and VR-2332 was identified with breakpoints in NSP2 and NSP10. Serological surveillance of 2043 vaccinated pigs showed an overall antibody positive rate of 83.0% (1696/2043), with increasing annual rates from 72.3% (2023) and 75.4% (2024) to 91.3% (2025). In conclusion, the PRRSV epidemic in southern Xinjiang is primarily driven by NADC30-like strains of PRRSV-2, with recombination events and GP5 epitope variations posing challenges to disease control. These findings enhance the epidemiological understanding of PRRSV in the region and provide valuable insights for vaccine development and prevention strategies.

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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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