Tissue engineering and regenerative medicine最新文献

{"title":"Stepwise Administration of Bone-Targeted Lipid Nanoparticles Encapsulating Valproic Acid and TUDCA Facilitates In Vivo Direct Reprogramming for Osteoporosis Treatment.","authors":"Hyoeun Park, Woong Jin Cho, Jiseong Kim, Hyejong Choi, Inho Baek, Youngjin Kim, Deogil Kim, Byoung Ju Kim, Yoshie Arai, Soo-Hong Lee","doi":"10.1007/s13770-025-00738-5","DOIUrl":"https://doi.org/10.1007/s13770-025-00738-5","url":null,"abstract":"<p><strong>Background: </strong>The ultimate goal of regenerative medicine is to restore damaged tissues to a healthy state in the body. Direct reprogramming, also referred to as transdifferentiation, holds significant therapeutic potential by converting abundant somatic cells, such as fibroblasts, into functionally distinct cell types for tissue regeneration. Despite its potential applications in regenerative medicine, direct reprogramming faces major challenges, including low efficiency and poor In vivo applicability. In this study, we propose a novel therapeutic strategy for osteoporosis based on In vivo direct reprogramming using a stepwise delivery approach that first enhances cellular stemness and subsequently induces osteogenic transdifferentiation. Enhancing stemness in lineage-committed cells facilitates their conversion into other functional cell types.</p><p><strong>Method: </strong>To investigate the efficiency of direct reprogramming via stepwise delivery, we utilized valproic acid (VPA) and tauroursodeoxycholic acid (TUDCA) as reprogramming and bone-stimulating factors, respectively. VPA increased the expression of stemness genes, including Oct4, Nanog, and Sox2, and subsequent treatment of TUDCA enhanced the expression of osteogenic genes in the mouse fibroblast. Targeted delivery of these factors to fibroblasts surrounding bone tissue, enabling subsequent direct reprogramming into osteoblasts, was achieved using bisphosphonate (BP)-conjugated lipid nanoparticles as carriers.</p><p><strong>Results: </strong>Our findings demonstrate that sequential induction of cell reprogramming and tissue regeneration through stepwise administration of VPA and TUDCA significantly enhances therapeutic efficacy in a mouse model of osteoporosis compared to their simultaneous administration.</p><p><strong>Conclusion: </strong>This stepwise bone-targeted drug delivery system presents a promising strategy for osteoporosis treatment via In vivo direct reprogramming.</p>","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotype-Preserving Co-culture of Osteoblasts and Chondrocytes Enhances Bone-Cartilage Interface Integration in a PRP-Augmented Scaffold.","authors":"Sunhyung Lee, Jinwoo Nam, Hong Seok Kim, Jeong Joon Yoo","doi":"10.1007/s13770-025-00727-8","DOIUrl":"https://doi.org/10.1007/s13770-025-00727-8","url":null,"abstract":"<p><strong>Background: </strong>Effective bone-cartilage integration remains a challenge in orthopedic surgery. Conventional methods often fail to reconstruct the native osteochondral interface. This study explores a scaffold-mediated approach utilizing co-cultured osteoblasts and chondrocytes, with platelet-rich plasma (PRP) as a potential promotor for bone-cartilage interface healing.</p><p><strong>Methods: </strong>We developed a co-culture system integrating both osteoblasts and chondrocytes on PLGA scaffolds, either with or without PRP supplementation. Cell phenotype maintenance was evaluated by RT-PCR, while morphological analysis was performed by scanning electron microscopy and fluorescence microscopy. To assess healing potential, we created a gap-mimic construct comprising bone, scaffold, and cartilage layers, which was implanted subcutaneously in BALB/c-nude mice. Gap healing was evaluated at 4 and 8 weeks through macroscopic examination, quantitative adhesion analysis, and histological assessment of cellular invasion.</p><p><strong>Results: </strong>Co-cultured osteoblasts and chondrocytes maintained their phenotypes on PLGA scaffolds, with PRP significantly enhancing cell adhesion (215% increase for chondrocytes, 120% for osteoblasts) and proliferation. In vivo, cell-containing scaffolds demonstrated significantly greater attachment at the bone-cartilage interface compared to acellular constructs. PRP-treated scaffolds exhibited higher attachment rates (82.3% vs 76.7%) and cellular invasion (5/6 vs 3/6 constructs) at 8 weeks, with invasion observed as early as 4 weeks in the PRP group, suggesting accelerated remodeling.</p><p><strong>Conclusion: </strong>This study demonstrates the feasibility of developing transplantable scaffolds containing co-cultured osteoblasts and chondrocytes while preserving their phenotypes. These scaffolds exhibit significant potential in promoting healing at the bone-cartilage interface, with PRP further enhancing proliferation and improving the scaffold's ability to promote bone-cartilage interface healing.</p>","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Effect of Stromal Vascular Fraction and Lymph Node Transfer in a Rabbit Hindlimb Model.","authors":"Jaemin Lee, Jihyun Kim, Jeong-Hyun Cheon, Hyung-Chul Lee, Jae-Ho Chung, Eul-Sik Yoon","doi":"10.1007/s13770-025-00728-7","DOIUrl":"https://doi.org/10.1007/s13770-025-00728-7","url":null,"abstract":"<p><strong>Background: </strong>Adipose-derived stem cells (ADSCs) promote lymphangiogenesis, though their integration with vascularized lymph node transfer (VLNT) is not well-explored. Unlike ADSCs, the stromal vascular fraction (SVF) can be obtained intraoperatively without the need for cell culture, making it ideal for incorporation into VLNT in a single-stage surgical procedure. This study evaluates the impacts of combined VLNT and SVF therapy using a rabbit hindlimb model.</p><p><strong>Method: </strong>New Zealand white rabbits were divided into four groups: control, VLNT only, SVF only, and combined VLNT plus SVF. The VLNT procedure involved transferring a pedicled lymph node flap, while the SVF was harvested and injected into the perinodal tissue. Postoperative assessments included measuring edema volume, performing ICG lymphography, conducting histological analysis, and measuring VEGF-C and LYVE-1 expression.</p><p><strong>Results: </strong>Initial increases in hindlimb edema volume were noted, but a significant decrease occurred by week 4, particularly in the VLNT group and VLNT plus SVF group compared to the control group. Histological evaluations indicated that the combined treatment group preserved superior structural integrity of the lymph nodes, with a decreased proportion of fibroadipose tissue compared to the VLNT-only group. Elevated VEGF-C expression was observed in the SVF-treated groups, as confirmed by both RT-PCR and ELISA analyses at week 4. Additionally, the combined VLNT plus SVF group showed increased LYVE-1 expression by week 8.</p><p><strong>Conclusion: </strong>The results suggest that SVF can be effectively integrated with VLNT in a single-stage procedure, enhancing the viability and structural integrity of vascularized lymph nodes. These results highlight the potential of this combined approach as a promising therapeutic strategy for advanced-stage lymphedema, meriting further exploration in clinical trials.</p>","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144294963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strategies for the Patient-Specific Implant Angle of Bone Scaffolds Using Optimization.","authors":"Jun Won Choi, Jung Jin Kim","doi":"10.1007/s13770-025-00730-z","DOIUrl":"https://doi.org/10.1007/s13770-025-00730-z","url":null,"abstract":"<p><strong>Background: </strong>Bone scaffolds are artificial structures used for restoring bone functionality via the reconstruction and repair of bone tissue. Although these scaffolds interact seamlessly with the surrounding tissue, conventional scaffold designs often fail to consider the microstructure of the surrounding bone, leading to reduced mechanical performance. This study proposed an implantation angle optimization approach for bone scaffolds that considers the microstructures around the implant, thus improving the mechanical properties of commonly used scaffolds.</p><p><strong>Method: </strong>This study proposed a novel method for optimizing the implantation angle of bone scaffolds, thereby enhancing their mechanical performance and integration with the surrounding bone tissue. A finite element model based on the imaging data of the bone scaffold within the skeletal system was constructed. Then, the structural behavior under external load was analyzed to determine the optimal implantation angle by rotating the bone scaffold.</p><p><strong>Result: </strong>Bone scaffolds with optimized angles show up to 7.53% strain energy difference between the scaffold and native bone, which improves load transfer and supports more natural bone remodeling. These results suggest that this approach enhances scaffold stability and reduces the risk of implant failure.</p><p><strong>Conclusion: </strong>The results highlight the potential of the proposed approach to optimize the implantation angle considering the bone microstructure, thus significantly enhancing scaffold performance. The combination of these strategies shows significant potential for advancing bone-repair solutions and improving patient outcomes in orthopedic surgeries.</p>","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144286536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanofiber-Based Biomimetic Platforms for Chronic Wound Healing: Recent Innovations and Future Directions.","authors":"Mina Kwon, Ki Su Kim","doi":"10.1007/s13770-025-00729-6","DOIUrl":"https://doi.org/10.1007/s13770-025-00729-6","url":null,"abstract":"<p><strong>Background: </strong>Wound healing remains a significant challenge in healthcare, particularly for complex and chronic wounds where conventional treatments often fail to provide effective solutions. Recent advances in nanofiber technology have opened new avenues for wound management by offering biomimetic structures that support tissue regeneration. Due to their high surface area-to-volume ratio and porosity, nanofibers closely resemble the extracellular matrix, facilitating an optimal environment for cell adhesion, proliferation, and differentiation.</p><p><strong>Methods: </strong>This review examines the role of nanofiber-based wound dressings, highlighting their unique advantages in drug delivery, moisture retention, and antimicrobial protection. Additionally, emerging trends such as smart wound dressings responsive to environmental stimuli and multifunctional nanofiber systems are discussed.</p><p><strong>Results and conclusion: </strong>Nanofiber technology has demonstrated significant potential in enhancing wound healing outcomes by providing an advanced platform for therapeutic delivery and tissue regeneration. Furthermore, the integration of nanofibers with artificial intelligence and biotechnology offers promising directions for future research. As these innovations continue to evolve, nanofiber-based wound dressings may revolutionize wound care by enabling more personalized and effective treatment strategies.</p>","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144286535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current Status of Stromal Vascular Fraction from Adipose Tissue in the Clinical Application for Osteoarthritis Treatment.","authors":"Gun-Il Im","doi":"10.1007/s13770-025-00722-z","DOIUrl":"https://doi.org/10.1007/s13770-025-00722-z","url":null,"abstract":"<p><strong>Background: </strong>Recently, regenerative medicine based on cell-based therapies has emerged as a therapeutic possibility for the management of osteoarthritis (OA). Stromal vascular fraction (SVF) is a cellular mixture obtained from lipoaspirate processed through either mechanical or enzymatic separation. SVF has been applied in several countries to treat OA patients without robust supporting evidence or comprehensive evaluation.</p><p><strong>Methods: </strong>This review purposes to summarize clinical evidence regarding SVF as a therapeutic for OA and to introduce the author's perspective. Eleven studies were found suitable for this review; out of these, seven were randomized clinical trials and four were cohort studies.</p><p><strong>Results: </strong>A review of controlled studies suggests that SVF may offer better symptomatic relief than placebo or hyaluronic acid in the long term, and the effect of SVF is comparable to that of bone marrow aspirate concentrates.</p><p><strong>Conclusion: </strong>Prospective studies with improved control over the cell isolation method, dosage, and patient selection are necessary to provide convincing evidence of the benefits of SVF in treating OA.</p>","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144235323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of Autotaxin-Inhibiting Lipid Nanoparticles to Regulate Autophagy and Inflammatory Responses in Activated Macrophages.","authors":"So Won Jeon, Jun Kwon, Hee Gyeong Ko, Jong Sang Yoon, Yun A Kim, Ju-Ro Lee, Min-Ho Kang, Han Young Kim","doi":"10.1007/s13770-025-00705-0","DOIUrl":"10.1007/s13770-025-00705-0","url":null,"abstract":"<p><strong>Background: </strong>Autotaxin (ATX), an ENPP2 enzyme, regulates lipid signaling by converting lysophosphatidylcholine to lysophosphatidic acid (LPA). Dysregulation of the ATX/LPA axis promotes inflammation and disease progression. BMP-22, a lipid ATX inhibitor, effectively reduces LPA production. However, its clinical utility is hampered by limitations in solubility and pharmacokinetics. To overcome these limitations, we developed BMP-22-incorporated lipid nanoparticles (LNP-BMP) to improve utility while maintaining ATX inhibition efficacy.</p><p><strong>Methods: </strong>LNP-BMP was synthesized by incorporating DOTAP, DOPE, cholesterol, 18:0 PEG₂₀₀₀-PE, and together with BMP-22. The formulation of LNP-BMP was optimized and characterized by testing different molar ratios of BMP-22. The autophagy recovery and anti-inflammatory effects of LNP-BMP via ATX inhibition were evaluated in both macrophage cell line and mouse-derived primary macrophages.</p><p><strong>Results: </strong>LNP-BMP was shown to retain its functionality as an ATX inhibitor and maintain the physical characteristics upon BMP-22 integration. Synthesized LNP-BMP exerted superior ability to inhibit ATX activity. When applied to M1-induced macrophages, LNP-BMP exhibited substantial anti-inflammatory effects and successfully restored autophagy activity.</p><p><strong>Conclusion: </strong>The results demonstrate that LNP-BMP effectively inhibits ATX, achieving both anti-inflammatory effects and autophagy restoration, highlighting its potential as a standalone immunotherapeutic agent. Furthermore, the capacity to load therapeutic drugs into this formulation offers promising opportunities for further therapeutic strategies.</p>","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"397-408"},"PeriodicalIF":4.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122970/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143493534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of Chitosan Oligosaccharide-Coated Nanostructured Lipid Carriers for the Sustained Release of Strontium Ranelate.","authors":"Hayeon Lim, Yoseph Seo, Sung Jun Min, Daehyeon Yoo, Dong Nyoung Heo, Il Keun Kwon, Taek Lee","doi":"10.1007/s13770-025-00713-0","DOIUrl":"10.1007/s13770-025-00713-0","url":null,"abstract":"<p><strong>Background: </strong>Strontium ranelate (SR) is an effective bone regeneration drug; however, its low bioavailability and strong hydrophilicity cause a strong cytotoxicity, venous thrombosis, and allergic reactions when administered in its free form. This study aims to enhance the SR bioavailability by utilizing nanostructured lipid carriers (NLC) as a drug delivery system (DDS).</p><p><strong>Methods: </strong>To improve the drug delivery efficiency and sustained release of the NLC, their surfaces were coated with chitosan oligosaccharide (COS), a natural polymer. The synthesis of COS-NLC was confirmed by measuring particle size and zeta potential, while surface morphology was evaluated using atomic force microscopy (AFM). SR loading efficiencies and release profiles were analyzed via reversed-phase high-performance liquid chromatography (RP-HPLC), and cytotoxicity was evaluated in mouse fibroblast L929 cells.</p><p><strong>Results: </strong>Particle characterization indicated that the COS coating slightly increased the particle size (i.e., from 128.99 ± 2.77 to 131.46 ± 2.13 nm) and zeta potential (i.e., from - 13.94 ± 0.49 to - 6.58 ± 0.32 mV) of the NLC. The COS-NLC exhibited a high SR-loading efficiency of ~ 86.31 ± 3.28%. An in vitro release test demonstrated an improved sustained release tendency of SR from the COS-NLC compared to that from the uncoated NLC. In cytotoxicity assays using L929 cells, the COS coating reduced the cytotoxicity of the formulated DDS, and the SR-COS-NLC exhibited a 1.4-fold higher cell regeneration effect than SR alone.</p><p><strong>Conclusion: </strong>These findings suggest that the developed COS-NLC serve as an effective and biocompatible DDS platform for the delivery of poorly bioavailable drugs.</p>","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"425-440"},"PeriodicalIF":4.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122978/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovations in Vascular Repair from Mechanical Intervention to Regenerative Therapies.","authors":"Hye-Min Park, Chae-Lin Kim, Dasom Kong, Seon-Hee Heo, Hyun-Ji Park","doi":"10.1007/s13770-024-00700-x","DOIUrl":"10.1007/s13770-024-00700-x","url":null,"abstract":"<p><strong>Background: </strong>Vascular diseases, including atherosclerosis and thrombosis, are leading causes of morbidity and mortality worldwide, often resulting in vessel stenosis that impairs blood flow and leads to severe clinical outcomes. Traditional mechanical interventions, such as balloon angioplasty and bare-metal stents, provided initial solutions but were limited by restenosis and thrombosis. The advent of drug-eluting stents improved short-term outcomes by inhibiting vascular smooth muscle cell proliferation, however, they faced challenges including delayed reendothelialization and late-stage thrombosis.</p><p><strong>Methods: </strong>This review highlights the progression from mechanical to biological interventions in treating vascular stenosis and underscores the need for integrated approaches that combine mechanical precision with regenerative therapies.</p><p><strong>Results: </strong>To address long-term complications, bioresorbable stents were developed to provide temporary scaffolding that gradually dissolves, yet they still encounter challenges with mechanical integrity and optimal degradation rates. Consequently, emerging therapies now focus on biological approaches, such as gene therapy, extracellular vesicle treatments, and cell therapies, that aim to promote vascular repair at the cellular level. These strategies offer the potential for true vascular regeneration by enhancing endothelialization, modulating immune responses, and stimulating angiogenesis.</p><p><strong>Conclusion: </strong>Integrating mechanical precision with regenerative biological therapies offers a promising future for treating vascular stenosis. A comprehensive approach combining these modalities could achieve sustainable vascular health.</p>","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"551-567"},"PeriodicalIF":4.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant Peptide-Based Nanocarriers for Delivering Wound Healing Agents.","authors":"Inseo Lee, Woo Hyun Kwon, Joo-Young Kim, Ha Kyeong Kim, Ji-Eun Kim, Yong-Beom Lim, Woo-Jin Jeong, Jun Shik Choi","doi":"10.1007/s13770-025-00701-4","DOIUrl":"10.1007/s13770-025-00701-4","url":null,"abstract":"<p><strong>Background: </strong>Curcumin, a well-known wound healing agent, faces clinical limitations due to its poor water solubility, rapid degradation, and short plasma half-life. To address these challenges, we developed a self-assembling peptide incorporating an antioxidant sequence (YGDEY), which is capable of not only delivering curcumin but also exhibiting additional bioactivity to enhance wound healing.</p><p><strong>Methods: </strong>An antioxidant nanocarrier was developed via peptide self-assembly. To design an amphiphilic peptide for the nanocarrier assembly, antioxidant peptide sequence (YGDEY) as the hydrophilic segment and the hydrophobic block (WLWL) were incorporated to single peptide molecule. The peptide's self-assembly behavior and curcumin encapsulation were initially analyzed. Subsequent evaluations included cytocompatibility, cellular uptake, and antioxidant activity.</p><p><strong>Results: </strong>Driven by strong interactions among their hydrophobic blocks (WLWL), the peptides formed well-defined nanostructures exhibiting high thermal stability. Furthermore, the encapsulation of curcumin within the micelle significantly improved its cellular penetration efficiency. When applied to fibroblast cells, the peptide-curcumin nanocomplexes exhibited synergistically enhanced antioxidant activity, which notably outperformed free curcumin and free peptide in scavenging reactive oxygen species.</p><p><strong>Conclusion: </strong>These findings highlight the potential of the designed peptide-based nanocarrier to overcome intrinsic limitations of curcumin and enhance its therapeutic efficacy, providing a promising strategy for advanced wound healing applications.</p>","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"441-451"},"PeriodicalIF":4.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122397/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143383361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}