Strontium-Doped Marine Collagen Membranes Promote Osteogenesis by Inducing M2 Macrophage Polarization.

IF 4.4 4区 医学 Q2 CELL & TISSUE ENGINEERING
Hao Xu, Xin Li, Wenxue Wang, Li Zhen, Baodong Zhao
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引用次数: 0

Abstract

Background: The design of bone biomaterials has shifted from promoting bone differentiation to "immune osteogenic coupling". Macrophages play a key role in immune regulation, with their polarization state critically shaping the bone tissue immune microenvironment. While collagen membranes, as classic guided bone regeneration (GBR) barriers, offer excellent biocompatibility and degradability, they lack inherent bone induction and immune regulation capabilities, limiting their use in complex bone defect repair.

Methods: In this study, we proposed a novel optimization strategy utilizing phase-transited lysozymes (PTL) incorporating strontium (Sr2+) into marine collagen membranes (Sr-PTL-MCM) and investigate their osteoimmunomodulatory effect through a series of experiments.

Results: Sr-PTL-MCM were successfully synthesized via the PTL technique and continuously released Sr2+ ions over 7 days. Sr-PTL-MCM can effectively induce macrophage polarization from the M0 to M2 phenotype, suppresses the secretion of inflammatory cytokines, thereby enhancing mBMSCs osteogenic differentiation. RNA-sequence analysis reveals that Sr-PTL-MCM promotes M2 polarization via JAK-STAT and MAPK signaling pathways. In vivo experiments confirm its ability to create a favorable bone immune microenvironment, promoting bone growth and regeneration.

Conclusion: In conclusion, incorporating Sr ions into collagen via PTL technique represents a promising approach for developing collagen membranes with immunomodulatory characteristics, thereby providing a novel and effective strategy for bone defect repair.

掺锶海洋胶原膜通过诱导M2巨噬细胞极化促进成骨。
背景:骨生物材料的设计已经从促进骨分化转向“免疫成骨耦合”。巨噬细胞在免疫调节中发挥着关键作用,其极化状态对骨组织免疫微环境的形成至关重要。虽然胶原膜作为经典的引导骨再生(GBR)屏障,具有良好的生物相容性和可降解性,但其缺乏固有的骨诱导和免疫调节能力,限制了其在复杂骨缺损修复中的应用。方法:在本研究中,我们提出了一种新的优化策略,利用结合锶(Sr2+)的相转移溶菌酶(PTL)进入海洋胶原膜(Sr-PTL-MCM),并通过一系列实验研究其骨免疫调节作用。结果:通过PTL技术成功合成了Sr-PTL-MCM,并在7天内连续释放Sr2+。Sr-PTL-MCM能有效诱导巨噬细胞从M0表型向M2表型极化,抑制炎性细胞因子的分泌,从而增强mBMSCs成骨分化。rna序列分析表明,Sr-PTL-MCM通过JAK-STAT和MAPK信号通路促进M2极化。体内实验证实其能够创造良好的骨免疫微环境,促进骨生长和再生。结论:综上所述,通过PTL技术将Sr离子注入胶原蛋白是一种很有前途的方法,可以开发具有免疫调节特性的胶原膜,从而为骨缺损修复提供一种新颖有效的策略。
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来源期刊
Tissue engineering and regenerative medicine
Tissue engineering and regenerative medicine CELL & TISSUE ENGINEERING-ENGINEERING, BIOMEDICAL
CiteScore
6.80
自引率
5.60%
发文量
83
审稿时长
6-12 weeks
期刊介绍: Tissue Engineering and Regenerative Medicine (Tissue Eng Regen Med, TERM), the official journal of the Korean Tissue Engineering and Regenerative Medicine Society, is a publication dedicated to providing research- based solutions to issues related to human diseases. This journal publishes articles that report substantial information and original findings on tissue engineering, medical biomaterials, cells therapy, stem cell biology and regenerative medicine.
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