Tissue engineering and regenerative medicine最新文献

Synthesis of Autotaxin-Inhibiting Lipid Nanoparticles to Regulate Autophagy and Inflammatory Responses in Activated Macrophages. 合成抑制 Autotaxin 的脂质纳米颗粒，以调节活化巨噬细胞的自噬和炎症反应。

IF 4.4 4区医学

Tissue engineering and regenerative medicine Pub Date : 2025-06-01 Epub Date: 2025-02-25 DOI: 10.1007/s13770-025-00705-0

So Won Jeon, Jun Kwon, Hee Gyeong Ko, Jong Sang Yoon, Yun A Kim, Ju-Ro Lee, Min-Ho Kang, Han Young Kim

{"title":"Synthesis of Autotaxin-Inhibiting Lipid Nanoparticles to Regulate Autophagy and Inflammatory Responses in Activated Macrophages.","authors":"So Won Jeon, Jun Kwon, Hee Gyeong Ko, Jong Sang Yoon, Yun A Kim, Ju-Ro Lee, Min-Ho Kang, Han Young Kim","doi":"10.1007/s13770-025-00705-0","DOIUrl":"10.1007/s13770-025-00705-0","url":null,"abstract":"Background: Autotaxin (ATX), an ENPP2 enzyme, regulates lipid signaling by converting lysophosphatidylcholine to lysophosphatidic acid (LPA). Dysregulation of the ATX/LPA axis promotes inflammation and disease progression. BMP-22, a lipid ATX inhibitor, effectively reduces LPA production. However, its clinical utility is hampered by limitations in solubility and pharmacokinetics. To overcome these limitations, we developed BMP-22-incorporated lipid nanoparticles (LNP-BMP) to improve utility while maintaining ATX inhibition efficacy.Methods: LNP-BMP was synthesized by incorporating DOTAP, DOPE, cholesterol, 18:0 PEG2000-PE, and together with BMP-22. The formulation of LNP-BMP was optimized and characterized by testing different molar ratios of BMP-22. The autophagy recovery and anti-inflammatory effects of LNP-BMP via ATX inhibition were evaluated in both macrophage cell line and mouse-derived primary macrophages.Results: LNP-BMP was shown to retain its functionality as an ATX inhibitor and maintain the physical characteristics upon BMP-22 integration. Synthesized LNP-BMP exerted superior ability to inhibit ATX activity. When applied to M1-induced macrophages, LNP-BMP exhibited substantial anti-inflammatory effects and successfully restored autophagy activity.Conclusion: The results demonstrate that LNP-BMP effectively inhibits ATX, achieving both anti-inflammatory effects and autophagy restoration, highlighting its potential as a standalone immunotherapeutic agent. Furthermore, the capacity to load therapeutic drugs into this formulation offers promising opportunities for further therapeutic strategies.","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"397-408"},"PeriodicalIF":4.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143493534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of Chitosan Oligosaccharide-Coated Nanostructured Lipid Carriers for the Sustained Release of Strontium Ranelate. 壳聚糖包被纳米脂质载体的构建及其对雷奈酸锶缓释的影响。

IF 4.4 4区医学

Tissue engineering and regenerative medicine Pub Date : 2025-06-01 Epub Date: 2025-03-12 DOI: 10.1007/s13770-025-00713-0

Hayeon Lim, Yoseph Seo, Sung Jun Min, Daehyeon Yoo, Dong Nyoung Heo, Il Keun Kwon, Taek Lee

{"title":"Construction of Chitosan Oligosaccharide-Coated Nanostructured Lipid Carriers for the Sustained Release of Strontium Ranelate.","authors":"Hayeon Lim, Yoseph Seo, Sung Jun Min, Daehyeon Yoo, Dong Nyoung Heo, Il Keun Kwon, Taek Lee","doi":"10.1007/s13770-025-00713-0","DOIUrl":"10.1007/s13770-025-00713-0","url":null,"abstract":"Background: Strontium ranelate (SR) is an effective bone regeneration drug; however, its low bioavailability and strong hydrophilicity cause a strong cytotoxicity, venous thrombosis, and allergic reactions when administered in its free form. This study aims to enhance the SR bioavailability by utilizing nanostructured lipid carriers (NLC) as a drug delivery system (DDS).Methods: To improve the drug delivery efficiency and sustained release of the NLC, their surfaces were coated with chitosan oligosaccharide (COS), a natural polymer. The synthesis of COS-NLC was confirmed by measuring particle size and zeta potential, while surface morphology was evaluated using atomic force microscopy (AFM). SR loading efficiencies and release profiles were analyzed via reversed-phase high-performance liquid chromatography (RP-HPLC), and cytotoxicity was evaluated in mouse fibroblast L929 cells.Results: Particle characterization indicated that the COS coating slightly increased the particle size (i.e., from 128.99 ± 2.77 to 131.46 ± 2.13 nm) and zeta potential (i.e., from - 13.94 ± 0.49 to - 6.58 ± 0.32 mV) of the NLC. The COS-NLC exhibited a high SR-loading efficiency of ~ 86.31 ± 3.28%. An in vitro release test demonstrated an improved sustained release tendency of SR from the COS-NLC compared to that from the uncoated NLC. In cytotoxicity assays using L929 cells, the COS coating reduced the cytotoxicity of the formulated DDS, and the SR-COS-NLC exhibited a 1.4-fold higher cell regeneration effect than SR alone.Conclusion: These findings suggest that the developed COS-NLC serve as an effective and biocompatible DDS platform for the delivery of poorly bioavailable drugs.","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"425-440"},"PeriodicalIF":4.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovations in Vascular Repair from Mechanical Intervention to Regenerative Therapies. 血管修复从机械干预到再生疗法的创新。

IF 4.4 4区医学

Tissue engineering and regenerative medicine Pub Date : 2025-06-01 Epub Date: 2025-02-08 DOI: 10.1007/s13770-024-00700-x

Hye-Min Park, Chae-Lin Kim, Dasom Kong, Seon-Hee Heo, Hyun-Ji Park

{"title":"Innovations in Vascular Repair from Mechanical Intervention to Regenerative Therapies.","authors":"Hye-Min Park, Chae-Lin Kim, Dasom Kong, Seon-Hee Heo, Hyun-Ji Park","doi":"10.1007/s13770-024-00700-x","DOIUrl":"10.1007/s13770-024-00700-x","url":null,"abstract":"Background: Vascular diseases, including atherosclerosis and thrombosis, are leading causes of morbidity and mortality worldwide, often resulting in vessel stenosis that impairs blood flow and leads to severe clinical outcomes. Traditional mechanical interventions, such as balloon angioplasty and bare-metal stents, provided initial solutions but were limited by restenosis and thrombosis. The advent of drug-eluting stents improved short-term outcomes by inhibiting vascular smooth muscle cell proliferation, however, they faced challenges including delayed reendothelialization and late-stage thrombosis.Methods: This review highlights the progression from mechanical to biological interventions in treating vascular stenosis and underscores the need for integrated approaches that combine mechanical precision with regenerative therapies.Results: To address long-term complications, bioresorbable stents were developed to provide temporary scaffolding that gradually dissolves, yet they still encounter challenges with mechanical integrity and optimal degradation rates. Consequently, emerging therapies now focus on biological approaches, such as gene therapy, extracellular vesicle treatments, and cell therapies, that aim to promote vascular repair at the cellular level. These strategies offer the potential for true vascular regeneration by enhancing endothelialization, modulating immune responses, and stimulating angiogenesis.Conclusion: Integrating mechanical precision with regenerative biological therapies offers a promising future for treating vascular stenosis. A comprehensive approach combining these modalities could achieve sustainable vascular health.","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"551-567"},"PeriodicalIF":4.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant Peptide-Based Nanocarriers for Delivering Wound Healing Agents. 基于抗氧化肽的伤口愈合剂纳米载体。

IF 4.4 4区医学

Tissue engineering and regenerative medicine Pub Date : 2025-06-01 Epub Date: 2025-02-10 DOI: 10.1007/s13770-025-00701-4

Inseo Lee, Woo Hyun Kwon, Joo-Young Kim, Ha Kyeong Kim, Ji-Eun Kim, Yong-Beom Lim, Woo-Jin Jeong, Jun Shik Choi

{"title":"Antioxidant Peptide-Based Nanocarriers for Delivering Wound Healing Agents.","authors":"Inseo Lee, Woo Hyun Kwon, Joo-Young Kim, Ha Kyeong Kim, Ji-Eun Kim, Yong-Beom Lim, Woo-Jin Jeong, Jun Shik Choi","doi":"10.1007/s13770-025-00701-4","DOIUrl":"10.1007/s13770-025-00701-4","url":null,"abstract":"Background: Curcumin, a well-known wound healing agent, faces clinical limitations due to its poor water solubility, rapid degradation, and short plasma half-life. To address these challenges, we developed a self-assembling peptide incorporating an antioxidant sequence (YGDEY), which is capable of not only delivering curcumin but also exhibiting additional bioactivity to enhance wound healing.Methods: An antioxidant nanocarrier was developed via peptide self-assembly. To design an amphiphilic peptide for the nanocarrier assembly, antioxidant peptide sequence (YGDEY) as the hydrophilic segment and the hydrophobic block (WLWL) were incorporated to single peptide molecule. The peptide's self-assembly behavior and curcumin encapsulation were initially analyzed. Subsequent evaluations included cytocompatibility, cellular uptake, and antioxidant activity.Results: Driven by strong interactions among their hydrophobic blocks (WLWL), the peptides formed well-defined nanostructures exhibiting high thermal stability. Furthermore, the encapsulation of curcumin within the micelle significantly improved its cellular penetration efficiency. When applied to fibroblast cells, the peptide-curcumin nanocomplexes exhibited synergistically enhanced antioxidant activity, which notably outperformed free curcumin and free peptide in scavenging reactive oxygen species.Conclusion: These findings highlight the potential of the designed peptide-based nanocarrier to overcome intrinsic limitations of curcumin and enhance its therapeutic efficacy, providing a promising strategy for advanced wound healing applications.","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"441-451"},"PeriodicalIF":4.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143383361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polynucleotide and Hyaluronic Acid Mixture for Skin Wound Dressing for Accelerated Wound Healing. 多核苷酸和透明质酸混合物用于皮肤伤口敷料，加速伤口愈合。

IF 4.4 4区医学

Tissue engineering and regenerative medicine Pub Date : 2025-06-01 Epub Date: 2025-02-26 DOI: 10.1007/s13770-025-00712-1

Tae-Hyun Heo, Bon Kang Gu, Kyungeun Ohk, Jeong-Kee Yoon, Young Hoon Son, Heung Jae Chun, Dae-Hyeok Yang, Gun-Jae Jeong

{"title":"Polynucleotide and Hyaluronic Acid Mixture for Skin Wound Dressing for Accelerated Wound Healing.","authors":"Tae-Hyun Heo, Bon Kang Gu, Kyungeun Ohk, Jeong-Kee Yoon, Young Hoon Son, Heung Jae Chun, Dae-Hyeok Yang, Gun-Jae Jeong","doi":"10.1007/s13770-025-00712-1","DOIUrl":"10.1007/s13770-025-00712-1","url":null,"abstract":"Background: Skin wound healing is a complex process requiring coordinated cellular and molecular interactions. Polynucleotides (PN) and hyaluronic acid (HA) have emerged as promising agents in regenerative medicine due to their ability to enhance cellular proliferation, angiogenesis, and extracellular matrix (ECM) remodeling. Combining PN and HA offers potential synergistic effects, accelerating wound repair.Methods: PN and HA hydrogels were prepared and evaluated for viscosity and gel stability. Their effects on human dermal fibroblasts (HDF) and keratinocytes (HaCaT) were assessed using migration, proliferation assays, and gene expression analyses for vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9), and matrix metalloproteinase-10 (MMP-10). In vivo studies were conducted using a mouse wound model to observe wound closure and tissue regeneration over 14 days.Results: The PN-HA mixture demonstrated superior mechanical stability compared to individual components. In vitro, PN-HA significantly enhanced HDF and HaCaT migration, proliferation, and upregulated VEGF, MMP-9, and MMP-10 expression. In vivo, PN-HA treatment accelerated wound closure, improved dermal thickness, and enhanced ECM remodeling, as evidenced by histological analyses.Conclusion: The PN-HA combination synergistically accelerates wound healing by promoting angiogenesis, cellular migration, and ECM remodeling. These findings highlight its potential as an advanced wound dressing for acute and chronic wound management.","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"515-526"},"PeriodicalIF":4.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Branched Polymer Architecture for Modulating Interactions in Material-Bio Interface. 用于调节材料-生物界面相互作用的支化聚合物结构。

IF 4.4 4区医学

Tissue engineering and regenerative medicine Pub Date : 2025-06-01 Epub Date: 2025-03-08 DOI: 10.1007/s13770-024-00699-1

Fahimeh Taghavimandi, Min Gyu Kim, Mingyu Lee, Kwangsoo Shin

{"title":"Branched Polymer Architecture for Modulating Interactions in Material-Bio Interface.","authors":"Fahimeh Taghavimandi, Min Gyu Kim, Mingyu Lee, Kwangsoo Shin","doi":"10.1007/s13770-024-00699-1","DOIUrl":"10.1007/s13770-024-00699-1","url":null,"abstract":"Background: Branched polymers, including star, dendrimers, comb, and biomimetic polymers, are increasingly recognized for their potential in tissue engineering. Their unique architectures and functional properties contribute to enhanced mechanical strength, bioactivity, and adaptability of scaffolds and hydrogels.Objective: This review explores the diverse applications of branched polymers in tissue engineering and regenerative medicine, emphasizing their role in mimicking the extracellular matrix (ECM) and modulating interactions at the material-bio interface. The structural features of branched polymers, including branching density and functional group distribution, are highlighted for their influence on drug delivery, mechanical properties, and cellular interactions.Results: Branched polymers offer distinct advantages in tissue engineering: Star polymers: Provide tunable elasticity and facilitate long-range mechanical networking. Dendrimers: Enable precise functionalization for targeted drug delivery and cell signaling. Comb polymers: Enhance porosity and nutrient exchange in scaffolds. Biomimetic polymers: Mimic natural biological systems, promoting cellular adhesion, proliferation, and differentiation.Conclusion: Branched polymers represent a versatile and promising platform for tissue engineering and regenerative medicine. Their ability to modulate biological interactions and adapt to diverse functional requirements underscores their potential to advance the field.","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"481-504"},"PeriodicalIF":4.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunomodulation Effects of Porcine Cartilage Acellularized Matrix (pCAM) for Osteoarthritis Treatment. 猪软骨脱细胞基质对骨关节炎的免疫调节作用。

IF 4.4 4区医学

Tissue engineering and regenerative medicine Pub Date : 2025-06-01 Epub Date: 2025-01-09 DOI: 10.1007/s13770-024-00687-5

Ji Seob Kim, Hyeon Jae Kwon, In Sun Hwang, Young Hwa Lee, Kyung-Noh Yoon, Hee-Woong Yun, Jae-Hyeok Jang, Seo Jeong Kim, Zhoodatova Aiana, Seungwoo Kim, Minhee Moon, Bongki Kim, Byoung Ju Kim, Byung-Hyun Cha

{"title":"Immunomodulation Effects of Porcine Cartilage Acellularized Matrix (pCAM) for Osteoarthritis Treatment.","authors":"Ji Seob Kim, Hyeon Jae Kwon, In Sun Hwang, Young Hwa Lee, Kyung-Noh Yoon, Hee-Woong Yun, Jae-Hyeok Jang, Seo Jeong Kim, Zhoodatova Aiana, Seungwoo Kim, Minhee Moon, Bongki Kim, Byoung Ju Kim, Byung-Hyun Cha","doi":"10.1007/s13770-024-00687-5","DOIUrl":"10.1007/s13770-024-00687-5","url":null,"abstract":"Background: Pain reduction, immunomodulation, and cartilage repair are key therapeutic goals in osteoarthritis (OA) treatment. In this study, we evaluated the therapeutic effects of porcine cartilage acellularized matrix (pCAM) derived from naive tissue and compared it with the synthetic material polynucleotides (PN) for OA treatment.Methods: pCAM was produced from porcine cartilage through physicochemical processing. LC-MS protein profiling identified the key proteins. In vitro experiments involved treating human synovial cell with pCAM and PN to assess cell viability and reductions in pro-inflammatory cytokines (IL-1β and IL-6). In vivo studies utilized a rat DMM-induced OA model. Pain was evaluated in weight-bearing tests, and inflammation reduction was confirmed using specific macrophage markers of CD68, CD86, and CD163 in immunohistochemical staining of synovial tissue. Cartilage regeneration was evaluated by histopathological analyses.Results: The major protein components of pCAM include factors integral to cartilage and ECM integrity. They also contain proteins that help reduce inflammation. In vitro studies revealed a decrease in pro-inflammatory cytokines and survival of synovial cells were observed. In vivo treatment with pCAM resulted in a reduction of pain and inflammation, while promoting cartilage regeneration, thereby accelerating the healing process in OA.Conclusion: Our findings suggest that pCAM may contribute to the treatment of OA by alleviating synovial inflammation and supporting cartilage regeneration, thereby addressing both the inflammatory and degenerative aspects of the disease.","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"453-467"},"PeriodicalIF":4.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Injectable Endoplasmin-Loaded Lipid Nanoparticles-Hydrogel Composite for Cartilage Regeneration. 可注射内质酶负载脂质纳米颗粒-水凝胶复合材料用于软骨再生。

IF 4.4 4区医学

Tissue engineering and regenerative medicine Pub Date : 2025-06-01 Epub Date: 2025-02-24 DOI: 10.1007/s13770-024-00698-2

Sumi Choi, Hyeongrok Choi, Jin Woong Chung, Su-Hwan Kim

{"title":"Injectable Endoplasmin-Loaded Lipid Nanoparticles-Hydrogel Composite for Cartilage Regeneration.","authors":"Sumi Choi, Hyeongrok Choi, Jin Woong Chung, Su-Hwan Kim","doi":"10.1007/s13770-024-00698-2","DOIUrl":"10.1007/s13770-024-00698-2","url":null,"abstract":"Background: Endoplasmin (ENPL), a heat shock protein 90 family member, promotes chondrogenic differentiation of stem cells by inhibiting ERK1/2 phosphorylation and inducing endoplasmic reticulum stress. However, its large size limits cellular uptake and therapeutic potential. To overcome this challenge, a cationic lipid nanoparticle (C_LNP) system was designed to deliver ENPL intracellularly, enhancing its effects on human tonsil-derived mesenchymal stem cells (hTMSCs).Methods: ENPL-loaded cationic lipid nanoparticles (ENPL_C_LNP) were synthesized to facilitate intracellular ENPL delivery. The delivery efficiency and cytotoxicity were assessed in vitro using hTMSCs. Additionally, ENPL_C_LNPs were incorporated into a hyaluronic acid and chondroitin sulfate-based injectable hydrogel and tested for chondrogenic differentiation potential in a mouse subcutaneous model.Results: ENPL_C_LNP achieved over 80% intracellular protein delivery efficiency with no cytotoxic effects. Co-cultured hTMSCs exhibited increased glycosaminoglycans (GAGs) and collagen expression over 21 days. In vivo, the hydrogel-embedded ENPL_C_LNP system enabled stable cartilage differentiation, evidenced by abundant cartilage-specific lacuna structures in regenerated tissue.Conclusion: Combining ENPL_C_LNP with an injectable hydrogel scaffold supports chondrogenic differentiation and cartilage regeneration, offering a promising strategy for cartilage tissue engineering.","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"409-424"},"PeriodicalIF":4.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Implantable Dental Barrier Membranes as Regenerative Medicine in Dentistry: A Comprehensive Review. 作为牙科再生医学的可植入牙屏障膜：全面回顾。

IF 4.4 4区医学

Tissue engineering and regenerative medicine Pub Date : 2025-06-01 Epub Date: 2025-02-24 DOI: 10.1007/s13770-025-00704-1

Siyuan Chen, Zhenzhen Wu, Ziqi Huang, Chao Liang, Sang Jin Lee

{"title":"Implantable Dental Barrier Membranes as Regenerative Medicine in Dentistry: A Comprehensive Review.","authors":"Siyuan Chen, Zhenzhen Wu, Ziqi Huang, Chao Liang, Sang Jin Lee","doi":"10.1007/s13770-025-00704-1","DOIUrl":"10.1007/s13770-025-00704-1","url":null,"abstract":"Background: Periodontitis and bone loss in the maxillofacial and dental areas pose considerable challenges for both functional and aesthetic outcomes. To date, implantable dental barrier membranes, designed to prevent epithelial migration into defects and create a favorable environment for targeted cells, have garnered significant interest from researchers. Consequently, a variety of materials and fabrication methods have been explored in extensive research on regenerative dental barrier membranes.Methods: This review focuses on dental barrier membranes, summarizing the various biomaterials used in membrane manufacturing, fabrication methods, and state-of-the-art applications for dental tissue regeneration. Based on a discussion of the pros and cons of current membrane strategies, future research directions for improved membrane designs are proposed.Results and conclusion: To endow dental membranes with various biological properties that accommodate different clinical situations, numerous biomaterials and manufacturing methods have been proposed. These approaches provide theoretical support and hold promise for advancements in dental tissue regeneration.","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"527-549"},"PeriodicalIF":4.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143484014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing Skin Regeneration Efficacy of Human Dermal Fibroblasts Using Carboxymethyl Cellulose-Coated Biodegradable Polymer. 利用羧甲基纤维素包覆的生物可降解聚合物增强人真皮成纤维细胞的皮肤再生功效

IF 4.4 4区医学

Tissue engineering and regenerative medicine Pub Date : 2025-06-01 Epub Date: 2024-11-23 DOI: 10.1007/s13770-024-00681-x

You Bin Lee, Dong-Hyun Lee, Youn Chul Kim, Suk Ho Bhang

{"title":"Enhancing Skin Regeneration Efficacy of Human Dermal Fibroblasts Using Carboxymethyl Cellulose-Coated Biodegradable Polymer.","authors":"You Bin Lee, Dong-Hyun Lee, Youn Chul Kim, Suk Ho Bhang","doi":"10.1007/s13770-024-00681-x","DOIUrl":"10.1007/s13770-024-00681-x","url":null,"abstract":"Background: Polylactic acid (PLA) is extensively used in the medical and cosmetic industries for skin regeneration and as a dermal filler due to its biocompatibility and biodegradability. However, the effectiveness of PLA as a cosmetic filler is limited by its slow degradation rate and poor cell attachment properties. Recent studies have focused on enhancing the performance of PLA by combining it with other materials. This study aimed to evaluate the performance of carboxymethyl cellulose (CMC), known for its high biocompatibility, in comparison with the widely used hyaluronic acid (HA).Methods: Two types of PLA-based particles, HA-PLA and CMC-PLA were synthesized by combining PLA with HA and CMC, respectively. After characterizing the particles, we evaluated cell adhesion and viability using human dermal fibroblasts and analyzed gene and protein expression related to cell attachment and angiogenic paracrine factors.Results: The CMC-PLA particles maintained a more uniform size distribution than the HA-PLA particles and exhibited superior cell adhesion properties. Cells attached on the CMC-PLA particles showed enhanced secretion of angiogenic paracrine factors, suggesting a potential improvement in therapeutic efficacy.Conclusion: CMC-PLA particles demonstrated superior cell adhesion and secretion capabilities compared with HA-PLA particles, indicating their potential for application in skin regeneration and tissue recovery. Further research, including in vivo studies, is required to fully explore and validate the therapeutic potential of CMC-PLA particles.","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"505-513"},"PeriodicalIF":4.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0