Small Methods最新文献_第8页

Precisely Targeting TGF-β1 Promoter G-Quadruplex for Expediting Maturation of Tumor Dendritic Cells to Overcome Immune Evasion. 精确靶向TGF-β1启动子g -四重体加速肿瘤树突状细胞成熟克服免疫逃避

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-05-29 DOI: 10.1002/smtd.202500650

Chenglong Ma, Yue Sun, Heying Yuan, Junlin Ya, Chunyu Wang, Chuanqi Zhao, Xiaogang Qu

{"title":"Precisely Targeting TGF-β1 Promoter G-Quadruplex for Expediting Maturation of Tumor Dendritic Cells to Overcome Immune Evasion.","authors":"Chenglong Ma, Yue Sun, Heying Yuan, Junlin Ya, Chunyu Wang, Chuanqi Zhao, Xiaogang Qu","doi":"10.1002/smtd.202500650","DOIUrl":"https://doi.org/10.1002/smtd.202500650","url":null,"abstract":"The maturation of dendritic cells (DCs) represents a pivotal determinant in tumor immunotherapy efficacy. In advanced tumors, the immune-suppressive transforming growth factor-beta 1 (TGF-β1) is frequently overexpressed and plays a central role in immune suppression. Elevated TGF-β1 levels significantly impair DCs maturation, leading to reduced expression of major histocompatibility complex (MHC) molecules and inflammatory cytokines, consequently diminishing DCs-mediated T cell activation. Here, this work first validates the presence of a stable G-quadruplex (G4) structure within the TGF-β1 promoter region. To minimize the off-target effects, this work establishes a system precisely targeting TGF-β1 promoter G4. Through CRISPR-mediated precise targeting of TGF-β1 promoter G4 by G4 ligand biotin-labeled pyridodicarboxamide (Bio-PDC), this work demonstrates its functional role in downregulating TGF-β1 expression. By combing ZIF-8 as the carrier of Bio-PDC, the downregulation of TGF-β1 is enhanced in a dual pathway, which subsequently facilitated DCs maturation. These results highlight the key regulatory role of TGF-β1 promoter G4 in DCs maturation and suggest its therapeutic potential as a synergistic target for enhancing immunotherapy efficacy by counteracting tumor immune evasion mechanisms.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500650"},"PeriodicalIF":10.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accelerated Crystallization of Zeolites under Ambient Conditions via Microplasma Electrochemistry. 环境条件下沸石的微等离子体电化学加速结晶。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-05-29 DOI: 10.1002/smtd.202500404

Nan Zhao, Liyuan Zheng, Yulin Sun, Luxi Zhang, Qi Qing, Shuang Liu, Zhe Wang, Xiao-Gen Xiong, Xiangke Wang, Yuexiang Lu

{"title":"Accelerated Crystallization of Zeolites under Ambient Conditions via Microplasma Electrochemistry.","authors":"Nan Zhao, Liyuan Zheng, Yulin Sun, Luxi Zhang, Qi Qing, Shuang Liu, Zhe Wang, Xiao-Gen Xiong, Xiangke Wang, Yuexiang Lu","doi":"10.1002/smtd.202500404","DOIUrl":"https://doi.org/10.1002/smtd.202500404","url":null,"abstract":"Zeolites are typically synthesized with hydrothermal methods at high temperatures with long reaction time, making the process energy-intensive. Here a convenient strategy is introduced for rapid (<60 min) synthesis of high crystallinity NaA zeolites under ambient conditions with microplasma electrochemistry (MIPEC). The resultant NaA zeolites enhance surface area and more structural defects compared to those produced by hydrothermal methods. The electron paramagnetic resonance study demonstrates that the microplasma anode can generate a hundred times more hydroxyl free radicals (·OH) than the microplasma cathode, thereby expediting the zeolite crystallization process. The density function theory calculations also suggest that the abundant ·OH radicals will lower the free energy barrier with the cooperative effect to accelerate the synthesis. The NaA zeolites demonstrate superior performance as adsorbents for heavy metal ions, boasting higher adsorption capacities and exceptional stability when compared with those prepared hydrothermally. Additionally, they serve as excellent supports of cobalt catalysts for Fischer-Tropsch synthesis, achieving low CH4 conversion rates and a high olefin/paraffin ratio in C2-4 products. With its operational simplicity, accessible equipment, rapid synthesis capabilities, and enhanced material properties, MIPEC stands out as a highly promising technique for the efficient production of zeolites, underscoring its extensive potential for widespread applications.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500404"},"PeriodicalIF":10.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Manifesting p-d Orbital Hybridization Through Strategic D-Band Engineering: A Pathway to Boosted Bifunctional HER/OER Electrochemical Performance in Self-Templated Co-SnO₂ Grown Over Co-SnS₂ Nanosheets. 通过战略性d波段工程实现p-d轨道杂化：在Co-SnS2纳米片上生长的自模板Co-SnO2提高双功能HER/OER电化学性能的途径

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-05-29 DOI: 10.1002/smtd.202500712

Manash P Nath, Tanmoy Kalita, Suvankar Deka, Manju Kumari Jaiswal, Abhishek Borborah, Dhruba Jyoti Kalita, Lakshi Saikia, Biswajit Choudhury

{"title":"Manifesting p-d Orbital Hybridization Through Strategic D-Band Engineering: A Pathway to Boosted Bifunctional HER/OER Electrochemical Performance in Self-Templated Co-SnO2 Grown Over Co-SnS2 Nanosheets.","authors":"Manash P Nath, Tanmoy Kalita, Suvankar Deka, Manju Kumari Jaiswal, Abhishek Borborah, Dhruba Jyoti Kalita, Lakshi Saikia, Biswajit Choudhury","doi":"10.1002/smtd.202500712","DOIUrl":"https://doi.org/10.1002/smtd.202500712","url":null,"abstract":"In this study, Co-doped SnO2 is synthesized atop the hexagonal CoS template (CoSS) via direct air calcination of as-synthesized Co-doped SnS2 (CoS) nanosheets. The structural evolution facilitated the emergence of Co2+ and Co3+ states, complemented by surface-adsorbed sulfur oxyanions (SO4 2-, HSO3 -, SO3 2-). CoSS deposited over carbon cloth (CoSS/CC) exhibited superior bifunctional HER and OER, demonstrating higher stability and efficiency than their CoS/CC counterparts. Notably, CoSS/CC||CoSS/CC shows the overall water splitting at a minimum cell voltage of 1.5 V, significantly lower than CoS/CC||CoS/CC. Mechanistically, the Co3+ states serve as catalytically active sites that enhance OER, while the synergistic interaction between Co3+ and the sulfur oxyanions promotes HER activities. Density functional theory (DFT) calculations revealed an upshifted d-band centre (ɛd) and enhanced metal-oxygen covalency (Δ) in CoSS, with superior charge transfer and p-d hybridization. ATR-FTIR, Raman, and XPS investigations confirmed surface reconstruction of CoSS/CC electrodes with enhanced electrical conductivity. It is related that a highly strained system of VO-CoSS has more unfilled electronic states near the Fermi level (ɛF) to facilitate a stable interaction with HER/OER intermediates. Overall, this study underscores the superior bifunctional electrocatalytic efficiency of CoSS/CC over CoS/CC, establishing it as a promising candidate for efficient overall water splitting.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500712"},"PeriodicalIF":10.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lego-Inspired Splicing of Modularized Vascular Channels. 受乐高启发的模块化血管通道拼接。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-05-29 DOI: 10.1002/smtd.202401928

Xiaoyan Zou, Shilu Zhu, Yifeng Xia, Jie Gao, Jiading Chen, Peng Yao, Min Ye, Shuwei Shen, Ronald X Xu

{"title":"Lego-Inspired Splicing of Modularized Vascular Channels.","authors":"Xiaoyan Zou, Shilu Zhu, Yifeng Xia, Jie Gao, Jiading Chen, Peng Yao, Min Ye, Shuwei Shen, Ronald X Xu","doi":"10.1002/smtd.202401928","DOIUrl":"https://doi.org/10.1002/smtd.202401928","url":null,"abstract":"Vascular channels embedded within tissue-engineered hydrogels play a crucial role in replicating natural physiological environments and facilitating the delivery of nutrients and removal of metabolic byproducts. Although present techniques provide diverse strategies to create vascular channels, the flexible and scalable construction of these channels within hydrogels remains a challenge. Here, inspired by Lego assembly, an innovative modular construction strategy is introduced for developing perfusable vascular channels within hydrogels. This approach involves the customized design and fabrication of individual modules featuring diverse vascular channel architectures, which can be flexibly assembled into large-scale hydrogel constructs with hierarchical vascular channels through splicing. As a proof of concept, gelatin-based constructs with vascular channels are spliced across multiple dimensions-1D, 2D, and 3D-to validate the flexibility and scalability of the splicing technique. These vascular hydrogel constructs are successfully perfused, and the interfacial strengths of the different spliced constructs are characterized. Furthermore, a functionalized construct capable of mimicking the vascular barrier function of human umbilical vein endothelial cells (HUVECs) is established, and determined arterial endothelial cell integrity and functionality under flow conditions. The innovative splicing technique offers new insights into the construction of large-scale in vitro vascularized tissues, paving the way for addressing specific tissue engineering needs.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2401928"},"PeriodicalIF":10.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cu/Li Heterotopic Doping to Enable Long-Term Cyclicity for P2-Type Nickel Manganese Based Sodium‑Ion Batteries. Cu/Li异位掺杂使p2型镍锰基钠离子电池具有长期循环性能。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-05-29 DOI: 10.1002/smtd.202500478

Xu Wang, Zixiang Yang, Mikhail Pugach, Ning Fu, Yujia Cai, Qinghua Zhang, Yang Hou, Zhizhen Ye, Jianguo Lu

{"title":"Cu/Li Heterotopic Doping to Enable Long-Term Cyclicity for P2-Type Nickel Manganese Based Sodium‑Ion Batteries.","authors":"Xu Wang, Zixiang Yang, Mikhail Pugach, Ning Fu, Yujia Cai, Qinghua Zhang, Yang Hou, Zhizhen Ye, Jianguo Lu","doi":"10.1002/smtd.202500478","DOIUrl":"https://doi.org/10.1002/smtd.202500478","url":null,"abstract":"P2-Na0.67Ni0.33Mn0.67O2 is regarded as a viable cathode material for sodium ion batteries due to its high theoretical energy density. However, it also has challenges that impair its electrochemical performance, like the P2-O2 phase transition, the strong Jahn-Teller distortion effect, and the Na+/vacancy ordering at high voltage. Here, an approach is proposed to use the Cu/Li binary doping modification of P2-Na0.67Ni0.33Mn0.67O2, which stabilizes the cathode structure by introducing Li into alkali metal sites and Cu at transition metal sites. Due to its anti-site doping, Li acts as an interlayer stationary point that suppresses the relative slip of the TMO2 layer under high pressure and produces a solid solution reaction that is nearly zero phase transition. The assembled full-cell devices with Na0.67Li0.1Ni0.18Cu0.05Mn0.67O2 cathode and commercial hard carbon anode can deliver a high energy density of 379.3 Wh kg-1. In addition, due to the proposed unique dual-site doping, the full-cell also exhibits excellent cycling stability, which maintains a capacity retention rate of 84% and 71% over 200 and 1000 cycles at 1 C and 10 C, respectively. As a result, the proposed doping technique offers an effective approach to designing cathode materials with excellent cycling stability.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500478"},"PeriodicalIF":10.7,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Introducing an Experimental Route to Identify and Unify Lab-Scale Redox-Flow Battery Cell Performances via Molar Fluxes and Cell Constants. 介绍了一种通过摩尔通量和电池常数来鉴定和统一实验室规模氧化还原液流电池性能的实验路线。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-05-28 DOI: 10.1002/smtd.202401670

Sebastian Fricke, Luuk Kortekaas, Martin Winter, Mariano Grünebaum

{"title":"Introducing an Experimental Route to Identify and Unify Lab-Scale Redox-Flow Battery Cell Performances via Molar Fluxes and Cell Constants.","authors":"Sebastian Fricke, Luuk Kortekaas, Martin Winter, Mariano Grünebaum","doi":"10.1002/smtd.202401670","DOIUrl":"https://doi.org/10.1002/smtd.202401670","url":null,"abstract":"Redox flow batteries (RFBs) are a promising technology for grid energy storage based on their high potential for scalability, design flexibility, high efficiency, and long durability, hence great effort has been invested in this area of research. However, due to the large differences in lab-scale RFB cell design and construction as well their operational performance, fundamental studies on innovative RFB components (e.g., active materials, separators, additives) compare poorly due to the lack of standard setups, settings, and procedures. This work introduces an experimental calibration route for aqueous as well as nonaqueous RFBs based on a simple mass transport model using molar fluxes, enabling one to compare dissimilar lab-scale RFB cell setups by introducing several RFB parameters: First, K1, which summarizes the operating parameters of an RFB to identify the critical ratio (K1critical) needed for efficient charge-discharge cycling using a simple overvoltage and charge efficiency evaluation; second, the RFB cell constant ζ, quantifying the influence of a lab-scale RFB setup on its performance; and finally, K2, ultimately enabling full comparison of (idealized) K1critical operating parameters across RFB cell setups.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2401670"},"PeriodicalIF":10.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporal Interrogation of Single Spheroids Using Multiplexed Nanoplasmonic-Fluorescence Imaging. 利用多路纳米等离子体荧光成像对单个球体进行时空探测。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-05-28 DOI: 10.1002/smtd.202500106

Saeid Ansaryan, Yung-Cheng Chiang, Yen-Cheng Liu, Jiayi Tan, Luis Francisco Lorenzo-Martín, Matthias P Lutolf, Genrich Tolstonog, Hatice Altug

{"title":"Spatiotemporal Interrogation of Single Spheroids Using Multiplexed Nanoplasmonic-Fluorescence Imaging.","authors":"Saeid Ansaryan, Yung-Cheng Chiang, Yen-Cheng Liu, Jiayi Tan, Luis Francisco Lorenzo-Martín, Matthias P Lutolf, Genrich Tolstonog, Hatice Altug","doi":"10.1002/smtd.202500106","DOIUrl":"https://doi.org/10.1002/smtd.202500106","url":null,"abstract":"Advances in organoid models, as ex vivo mini-organs, and the development of screening imaging technologies have continuously driven each other forward. A complete understanding of organoids requires detailed insights into the intertwined intraorganoid and extraorganoid activities and how they change across time and space. This study introduces a multiplexed imaging platform that integrates label-free nanoplasmonic biosensing with fluorescence microscopy to offer simultaneous monitoring of dynamics occurring within and around arrays of single spheroids with spatiotemporal resolution. The label-free module employs nanoplasmonic biosensors with extraordinary optical transmission to track biomolecular secretions into the surroundings, while concurrent fluorescence imaging enables structural analysis and viability assessment. To perform multiparametric interrogation of the data from different channels over extended periods, a deep-learning-augmented image analysis is incorporated. The platform is applied to tumor spheroids to investigate vascular endothelial growth factor A secretion alongside morphometric changes and viability, showcasing its ability to capture variations in secretion and growth dynamics between untreated and drug-treated groups. This integrated approach advances comprehensive insights into organoid models and can complement existing technologies to accelerate discoveries in disease modeling and drug development.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500106"},"PeriodicalIF":10.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid Nanoparticles for Delivery of CRISPR Gene Editing Components. 用于递送CRISPR基因编辑组件的脂质纳米颗粒。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-05-28 DOI: 10.1002/smtd.202401632

Fan Wu, Nei Li, Yudian Xiao, Rohan Palanki, Hannah Yamagata, Michael J Mitchell, Xuexiang Han

引用次数: 0

StableTi₃C₂T_x MXene Ink Formulation and High-Resolution Aerosol Jet Printing for High-Performance MXene Supercapacitors. 用于高性能MXene超级电容器的StableTi3C2Tx MXene油墨配方和高分辨率气溶胶喷射打印。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-05-28 DOI: 10.1002/smtd.202500499

Fereshteh Rajabi Kouchi, Tony Valayil Varghese, Hailey Burgoyne, Naqsh E Mansoor, Myeong-Lok Seol, Nicholas McKibben, Shruti Nirantar, Karthik Chinnathambi, Josh Eixenberger, Olivia Maryon, Christopher E Shuck, Yury Gogotsi, Jessica E Koehne, David Estrada

{"title":"StableTi3C2Tx MXene Ink Formulation and High-Resolution Aerosol Jet Printing for High-Performance MXene Supercapacitors.","authors":"Fereshteh Rajabi Kouchi, Tony Valayil Varghese, Hailey Burgoyne, Naqsh E Mansoor, Myeong-Lok Seol, Nicholas McKibben, Shruti Nirantar, Karthik Chinnathambi, Josh Eixenberger, Olivia Maryon, Christopher E Shuck, Yury Gogotsi, Jessica E Koehne, David Estrada","doi":"10.1002/smtd.202500499","DOIUrl":"https://doi.org/10.1002/smtd.202500499","url":null,"abstract":"Lightweight energy storage devices are essential for developing compact wearable and distributed electronics, and additive manufacturing offers a scalable, low-cost approach to fabricating such devices with complex geometries. However, additive manufacturing of high-performance, on-demand energy storage devices remains challenging due to the need for stable, multifunctional nanomaterial inks. Herein, the development of 2-dimensional (2D) titanium carbide (Ti3C2Tx MXene) ink that is compatible with aerosol jet printing for energy storage applications is demonstrated. The developed MXene ink demonstrates long-term chemical and physical stability, ensuring consistent printability and achieving high-resolution prints (≈45 µm width lines) with minimal overspray. The high-resolution aerosol-jet printed MXene supercapacitor achieves an areal capacitance of 122 mF cm-2 and a volumetric capacitance of 611 F cm-3, placing them among the highest-performing printed supercapacitors reported to date. These findings highlight the potential of aerosol jet printing with MXene inks for on-demand, scalable, and cost-effective fabrication of printed electronic and electrochemical devices.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500499"},"PeriodicalIF":10.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interpretable Differential Abundance Signature (iDAS). 可解释差异丰度特征（iDAS）。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-05-27 DOI: 10.1002/smtd.202500572

Lijia Yu, Yingxin Lin, Xiangnan Xu, Pengyi Yang, Jean Y H Yang

{"title":"Interpretable Differential Abundance Signature (iDAS).","authors":"Lijia Yu, Yingxin Lin, Xiangnan Xu, Pengyi Yang, Jean Y H Yang","doi":"10.1002/smtd.202500572","DOIUrl":"https://doi.org/10.1002/smtd.202500572","url":null,"abstract":"Single-cell technologies have revolutionized the understanding of cellular dynamics by allowing researchers to investigate individual cell responses under various conditions, such as comparing diseased versus healthy states. Many differential abundance methods have been developed in this field, however, the understanding of the gene signatures obtained from those methods is often incomplete, requiring the integration of cell type information and other biological factors to yield interpretable and meaningful results. To better interpret the gene signatures generated in the differential abundance analysis, iDAS is developed to classify the gene signatures into multiple categories. When applied to melanoma single-cell data with multiple cell states and treatment phenotypes, iDAS identified cell state- and treatment phenotype-specific gene signatures, as well as interaction effect-related gene signatures with meaningful biological interpretations. The iDAS model is further applied to a longitudinal study and spatially resolved omics data to demonstrate its versatility in different analytical contexts. These results demonstrate that the iDAS framework can effectively identify robust, cell-state specific gene signatures and is versatile enough to accommodate various study designs, including multi-factor longitudinal and spatially resolved data.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500572"},"PeriodicalIF":10.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0