Small Methods最新文献_第7页

Self-Disassembling Macroporous Metal-Organic Framework-Based Micromotors with Magnetically Controlled Motion for Sequential Drug Release. 基于磁控运动的自拆卸大孔金属-有机框架微电机的顺序药物释放。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-06-03 DOI: 10.1002/smtd.202500724

Javier Bujalance Fernández, Víctor de la Asunción-Nadal, Beatriz Jurado Sánchez, Alberto Escarpa

{"title":"Self-Disassembling Macroporous Metal-Organic Framework-Based Micromotors with Magnetically Controlled Motion for Sequential Drug Release.","authors":"Javier Bujalance Fernández, Víctor de la Asunción-Nadal, Beatriz Jurado Sánchez, Alberto Escarpa","doi":"10.1002/smtd.202500724","DOIUrl":"https://doi.org/10.1002/smtd.202500724","url":null,"abstract":"Herein, the synthesis of macroporous zeolitic imidazole framework (ZIF)-based magnetic micromotors (MMs) for the dual encapsulation of 5-fluorouracil (5-FU) and doxorubicin (DOX), with pH-triggered release is described. The MMs are synthesized in methanolic solutions, using superparamagnetic iron oxide nanoparticles (Fe₃O₄) as seeding agents and magnetic engines. The macroporous structure facilitates the loading of high doses of 5-FU, while DOX is docked on the surface of the MMs. This results in release rates of 72.8 µg mg MMs⁻¹ of 5-FU and 3 µg mg MMs⁻¹ of DOX. The magnetic motion of the MMs enables targeted delivery to predefined locations, allowing interaction with cancer cells in the culture medium. Under acidic conditions, DOX becomes protonated and is released from the surface of the micromotors, demonstrating rapid-release kinetics. Simultaneously, the ZIF-8 structure degrades under these conditions, enabling the sustained release of 5-FU for continuous treatment. This dual-delivery, pH-induced mechanism is demonstrated in vitro using Caco-2 cells as a relevant biological model, revealing a decrease in viability and diffusion of the drugs released into the cells. This is the first magnetic metal-organic framework (MOF)-based MM that is pH-sensitive and capable of sequentially releasing two drugs.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500724"},"PeriodicalIF":10.7,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regularized Gradient Statistics Improve Generative Deep Learning Models of Super Resolution Microscopy. 正则化梯度统计改进超分辨率显微镜的生成深度学习模型。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-06-02 DOI: 10.1002/smtd.202401900

Meri Abgaryan, Xinning Cui, Nandu Gopan, Gabriel Della Maggiora, Artur Yakimovich, Ivo F Sbalzarini

{"title":"Regularized Gradient Statistics Improve Generative Deep Learning Models of Super Resolution Microscopy.","authors":"Meri Abgaryan, Xinning Cui, Nandu Gopan, Gabriel Della Maggiora, Artur Yakimovich, Ivo F Sbalzarini","doi":"10.1002/smtd.202401900","DOIUrl":"https://doi.org/10.1002/smtd.202401900","url":null,"abstract":"It is shown that regularizing the signal gradient statistics during training of deep-learning models of super-resolution fluorescence microscopy improves the generated images. Specifically, regularizing the images in the training data set is proposed to have gradient and Laplacian statistics closer to those expected for natural-scene images. The BioSR data set of matched pairs of diffraction-limited and super-resolution images is used to evaluate the proposed regularization in a state-of-the-art generative deep-learning model of super-resolution microscopy, the Conditional Variational Diffusion Model (CVDM). Since the proposed regularization is applied as a preprocessing step to the training data, it can be used in conjunction with any supervised machine-learning model. However, its utility is limited to images for which the prior is appropriate, which in the BioSR data set are the images of filamentous structures. The quality and generalization power of CVDM trained with and without the proposed regularization are compared, showing that the new prior yields images with clearer visual detail and better small-scale structure.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2401900"},"PeriodicalIF":10.7,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Controllable Liquid Metal Microparticles Production and Patterning by Miniaturized Filter-Sieve Generators. 可控制液态金属微粒的微型过滤-筛发生器生产与成形。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-06-02 DOI: 10.1002/smtd.202500301

Qingtian Zhang, Hongda Lu, Yipu Guo, Xiangbo Zhou, Liping Gong, Zexin Chen, Jialu Wang, Haiping Du, Shi-Yang Tang, Weihua Li

{"title":"Controllable Liquid Metal Microparticles Production and Patterning by Miniaturized Filter-Sieve Generators.","authors":"Qingtian Zhang, Hongda Lu, Yipu Guo, Xiangbo Zhou, Liping Gong, Zexin Chen, Jialu Wang, Haiping Du, Shi-Yang Tang, Weihua Li","doi":"10.1002/smtd.202500301","DOIUrl":"https://doi.org/10.1002/smtd.202500301","url":null,"abstract":"Liquid metal microparticles (LMMPs) with excellent conductivity and reactivity hold significant promise for applications in flexible electronics and sensors. However, current LMMP production methods face critical challenges, including achieving smaller particle sizes with low energy consumption, streamlining processes, and enhancing productivity. Herein, leveraging the tunable surface tension of LM droplets, a compact platform called the miniaturized filter-sieve generator (MFSG) is presented, for scalable, energy-efficient, and controllable LMMP production. The MFSG demonstrates high energy efficiency (average power consumption of 0.21 W) and productivity (6.51 × 105 particles per minute) while enabling precise control of microparticle sizes (2-300 µm). Furthermore, the MFSG can uniformly produce LMMPs with varied compositions. Harnessing these capabilities, a reconfigurable platform integrating the MFSG is developed to enable complex droplet patterning and an LMMP-based humidity sensor with high sensitivity. This innovative platform for on-demand LMMP production with low energy consumption will drive significant advancements in electronic devices and sensing systems.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500301"},"PeriodicalIF":10.7,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the Impact of Conductive Additives on the EPR Spectra of Hard Carbon Anodes. 导电添加剂对硬碳阳极EPR谱影响的评价。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-06-02 DOI: 10.1002/smtd.202500786

Wanli Wang, Yujie Xu, Yuqi Li, Yi Sun, Alistair J Fielding, Pawin Iamprasertkun, Junwei Yang, Bin Wang, Qiang Li, Mingbo Wu, Han Hu

{"title":"Evaluation of the Impact of Conductive Additives on the EPR Spectra of Hard Carbon Anodes.","authors":"Wanli Wang, Yujie Xu, Yuqi Li, Yi Sun, Alistair J Fielding, Pawin Iamprasertkun, Junwei Yang, Bin Wang, Qiang Li, Mingbo Wu, Han Hu","doi":"10.1002/smtd.202500786","DOIUrl":"https://doi.org/10.1002/smtd.202500786","url":null,"abstract":"Hard carbon (HC), a prime anode candidate for sodium-ion batteries, exhibits unresolved charge-state-microstructure debates reveal critical sodium storage mechanism gaps. This study employs electron paramagnetic resonance (EPR) spectroscopy as a principal characterization tool, capitalizing on its unique capability to probe electronic configurations and detect subtle structural transformations in carbon matrices. Through systematic EPR investigations of sodium storage dynamics in varied carbon architectures, the quasi-metallic state of sodium ions stored in closed pores exhibits distinct signal characteristics due to size effect-induced structural confinement, compared to surface storage mechanisms. Furthermore, the underappreciated influence of conductive carbon additives, a ubiquitous component in electrode formulations is specifically addressed, on spectroscopic interpretations. This findings reveal that sodium's distinctive storage states (ionic vs quasi-metallic) and their spatial distribution within carbon matrices induce quantifiable modifications in EPR spectral parameters, including characteristic linewidth broadening and lineshape evolution. The comparative analysis demonstrates that trace amounts (≤10 wt.%) of conductive additives can substantially distort ex situ EPR measurements, with interference patterns exhibiting strong material-dependent behavior. Therefore, the application of conductive additives demands rigorous consideration in EPR-based investigations of energy-storing carbon materials, given their methodological implications.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500786"},"PeriodicalIF":10.7,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Macrophage Vesicles-Loaded NIR-II AIEgens for Precise In Situ Fluorescence Imaging of Atherosclerosis. 巨噬细胞小泡装载NIR-II抗原用于动脉粥样硬化的精确原位荧光成像。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-06-02 DOI: 10.1002/smtd.202500486

Zhiheng Luo, Xipeng Li, Caifa You, Laiping Fang, Delong Yang, Yunxia Wu, Zhijin Fan, Qin Zeng, Xu Chen, Hua Ma, Junhui Zhao, Shuo Sang, Hongju Yang, Dong Wang, Judun Zheng, Yuhui Liao

{"title":"Macrophage Vesicles-Loaded NIR-II AIEgens for Precise In Situ Fluorescence Imaging of Atherosclerosis.","authors":"Zhiheng Luo, Xipeng Li, Caifa You, Laiping Fang, Delong Yang, Yunxia Wu, Zhijin Fan, Qin Zeng, Xu Chen, Hua Ma, Junhui Zhao, Shuo Sang, Hongju Yang, Dong Wang, Judun Zheng, Yuhui Liao","doi":"10.1002/smtd.202500486","DOIUrl":"https://doi.org/10.1002/smtd.202500486","url":null,"abstract":"Precise diagnosis of atherosclerosis (AS) is a crucial strategy for reducing acute cardiovascular events. Herein, a macrophage membrane (MM)-camouflaged biomimetic vesicle loaded with near-infrared-II (NIR-II) aggregation-induced emission luminogens (AIEgens) (P4TT@MM) is proposed to achieve localized in vivo imaging of atherosclerosis. The AIEgens characteristics of P4TT@MM effectively compensate for the fluorescence intensity decrease caused by the intramolecular torsional charge transfer (TICT) effect, even in foam cell-rich lesions. Benefiting from the biomimetic camouflage of MM and its strong affinity for atherosclerotic plaques, P4TT@MM enables precise targeting of lesion sites, successfully achieving high-contrast imaging of atherosclerotic plaques in the carotid arteries and aortas of the AS mice model. Compared with free P4TT, the NIR-II fluorescence intensity of P4TT@MM is increased by 140% in carotid arteries and 152% in aortas, respectively. Moreover, the P4TT@MM demonstrates robust biocompatibility combined with significant clinical translation potential. This study may present a promising tool for the clinical diagnosis of AS.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500486"},"PeriodicalIF":10.7,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A General Approach to Predict and Tailor the Nanoscale Permeability of Comb-Shaped Polymer Coatings. 预测和定制梳状聚合物涂层纳米级渗透率的一般方法。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-06-02 DOI: 10.1002/smtd.202500140

Nicole Drossis, Marc A Gauthier, Hendrick W de Haan

{"title":"A General Approach to Predict and Tailor the Nanoscale Permeability of Comb-Shaped Polymer Coatings.","authors":"Nicole Drossis, Marc A Gauthier, Hendrick W de Haan","doi":"10.1002/smtd.202500140","DOIUrl":"https://doi.org/10.1002/smtd.202500140","url":null,"abstract":"Comb-shaped polymers such as poly(oligoethylene glycol monomethyl ether) methacrylate) (pOEGMA) are used to produce molecular sieving coatings on proteins. The mechanisms and phenomena implicated in the experimentally observed sieving properties have been recently characterized by simulation. These result from an interplay between steric hindrance, microenvironmental effects, and modified protein dynamics. Steric hindrance, in particular, is expected to vary considerably as a function of the geometric parameters of the system (protein size, polymer size, grafting density, etc.). In this work, the steric and size-selective permeability characteristics of comb-polymer coatings are systematically explored across a very broad parameter space to gain a universal and predictive view of molecular sieving, for application to systems with different dimensions. All features of the data can be understood when three distinct regimes are considered: i) no-interactions, ii) weak-interactions, and iii) strong-interactions between adjacent polymer chains. Primary and secondary considerations are provided for tuning coating properties to adjust the size threshold of molecular sieving. The corresponding qualitative physical pictures and quantitative analyses give a robust framework to understand molecular sieving that will accelerate the development of future coatings.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500140"},"PeriodicalIF":10.7,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disorder-Induced Localization With on-Device Tunability in Asymmetric Molecular Semiconductors. 非对称分子半导体中具有器件内可调性的无序诱导定位。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-06-01 DOI: 10.1002/smtd.202500589

Kuakua Lu, Qijing Wang, Zhonglin Zhang, Xinglong Ren, Ian E Jacobs, Jingsi Qiao, Yi Shi, Yun Li, Henning Sirringhaus

引用次数: 0

Self-Assembled Fluorescent Peptide Nanoprobes for Disease Diagnosis. 自组装荧光肽纳米探针用于疾病诊断。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-06-01 DOI: 10.1002/smtd.202500518

Ruichen Wang, Tianwen Xi, Kaixuan Li, Chenyang Wang, Xing Gao, Hui Yang, Jianhua Zou, Xiaoyuan Chen, Leming Sun

{"title":"Self-Assembled Fluorescent Peptide Nanoprobes for Disease Diagnosis.","authors":"Ruichen Wang, Tianwen Xi, Kaixuan Li, Chenyang Wang, Xing Gao, Hui Yang, Jianhua Zou, Xiaoyuan Chen, Leming Sun","doi":"10.1002/smtd.202500518","DOIUrl":"https://doi.org/10.1002/smtd.202500518","url":null,"abstract":"Benefiting from the advantages of simple reactions, low energy consumption, and uniform properties, self-assembly has been widely utilized for preparation of nanoprobes. Peptides have been chosen as \"bricks\" for self-assembling nano-biomaterials due to their easy drug formation, highly variable sequence, good biocompatibility and biodegradability. Peptides can efficiently self-assemble through noncovalent interactions, such as hydrogen bonding, electrostatic interactions, π-π stacking, hydrophobic interactions, and van der Waals forces. Based on this, many ex situ and in situ self-assembly strategies are developed. The former self-assembles into stable nanomaterials beforehand, while the latter undergoes in situ self-assembly at the target site in response to its specific stimulus-responsive modules. As probe sizes are miniaturized to the nanometer scale, they gain high sensitivity, low detection limits, and the capability for in situ detection. Nanoprobes created through self-assembly, usually possess targeted accumulation in specific tissues, prolonged elimination half-life, and multimodal imaging capabilities, making them highly effective for disease diagnosis. This review introduces the types of self-assembled fluorescent peptide nanoprobes according to the source of fluorescence properties and summarizes the progress of their application in disease diagnosis, such as cancer, neurodegenerative disease, and bacterial infection. In addition, their limitations will be discussed, and new strategies will be proposed for the development of advanced peptide-based fluorescent self-assembling nanoprobes, aimed at improving their potential for clinical translation.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500518"},"PeriodicalIF":10.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bi-Continuous W-Rich Refractory High Entropy Alloy-Cu Composite: Toward Material Innovation of Nuclear Reactor Coolant System. 双连续富w耐火高熵合金- cu复合材料：核反应堆冷却剂系统材料创新之路。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-05-30 DOI: 10.1002/smtd.202500667

Kook Noh Yoon, Il Hwan Kim, Ji Young Kim, Peter Hosemann, Eun Soo Park

{"title":"Bi-Continuous W-Rich Refractory High Entropy Alloy-Cu Composite: Toward Material Innovation of Nuclear Reactor Coolant System.","authors":"Kook Noh Yoon, Il Hwan Kim, Ji Young Kim, Peter Hosemann, Eun Soo Park","doi":"10.1002/smtd.202500667","DOIUrl":"https://doi.org/10.1002/smtd.202500667","url":null,"abstract":"Refractory high-entropy alloys (RHEAs) are considered promising candidate materials for next-generation nuclear reactors due to their superior mechanical strength, irradiation resistance, and thermal stability at high temperatures. However, the significant positive heat of mixing between refractory alloying elements and Cu, commonly used in cooling systems, poses challenges in forming composite structures. This study addresses the issue using a liquid metal dealloying (LMD) process. A precursor alloy (WTaVTi) with a directional dendrite-interdendrite structure is fabricated and reacted with molten Cu at 1200 °C for 96 h. This approach produced a RHEA-Cu composite with a stable interface between RHEA (W31.5Ta30.9V21.4Ti14.3) and Cu, featuring a spontaneously formed W-rich interlayer that enhances interfacial bonding. The composite showed excellent irradiation resistance, with 30% less swelling under α-ion irradiation than pure W. It also exhibited low thermal conductivity at room temperature, but reached ≈120 W m-1·K-1 at ≈650 °C, surpassing pure W. This temperature-dependent rise in κ, with a positive gradient of +0.075 W m-1·K- 2, is attributed to decreasing diffuse mismatch at elevated temperatures. The large-scale reaction and stable microstructure achieved through LMD process highlight its industrial potential. This work offers a strategy for developing high-performance materials by combining RHEA's radiation resistance with Cu's thermal conductivity for extreme environments.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500667"},"PeriodicalIF":10.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Atom Cu Anchoring on Heterocyclic Ring of Metal-Organic Frameworks for Photocatalytic Nitrogen Fixation. 金属-有机骨架杂环单原子铜锚定光催化固氮。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-05-30 DOI: 10.1002/smtd.202500493

Jingyi Qu, Zhexiao Zhu, Xiaolu Xu, Jiahui Lin, Yangben Chen, Can Sun, Shouxin Zhu, Zijie Fang, Min Jiang, Hui Zheng

引用次数: 0