Small Methods最新文献_第6页

Ultralow-Noise Perovskite Photodiodes Enabling High-Fidelity Full-Color Imaging 实现高保真全彩成像的超低噪声钙钛矿光电二极管。

IF 8.7 2区材料科学

Small Methods Pub Date : 2026-10-07 Epub Date: 2026-07-12 DOI: 10.1002/smtd.70853

Chun-Jen Shih, Galing Murokinas, Meng-Chi Li, Shun-Wei Liu

{"title":"Ultralow-Noise Perovskite Photodiodes Enabling High-Fidelity Full-Color Imaging","authors":"Chun-Jen Shih, Galing Murokinas, Meng-Chi Li, Shun-Wei Liu","doi":"10.1002/smtd.70853","DOIUrl":"10.1002/smtd.70853","url":null,"abstract":"<div>\u0000 \u0000 Perovskite photodiodes have attracted increasing attention for next-generation imaging and sensing; however, their practical deployment remains hindered by elevated noise, device-to-device variability, and limited operational reliability compared to established silicon technologies. These limitations largely originate from interfacial defects and uncontrolled carrier recombination in solution-processed perovskite films. Here we present a vacuum-engineered perovskite photodiode architecture that enables ultralow-noise operation with high reproducibility and imaging-level performance. By constructing the perovskite structure through a controlled multilayer design, interfacial defect density is effectively suppressed, leading to reduced electrical noise and stable charge transport. The resulting devices exhibit an ultralow dark current density of 2.31 × 10−11 A cm−2 at 0.2 V, external quantum efficiency exceeding 80% across the visible spectrum, and a wide linear dynamic range of 130 dB, together with a −3 dB bandwidth of 0.33 MHz. These performance characteristics enable high-fidelity full-color imaging using a single-pixel scanning approach, demonstrating accurate image reconstruction with low noise. The devices further maintain over 80% of their initial performance after 500 h of continuous illumination, highlighting their operational stability. This work establishes a general strategy for achieving low-noise and reliable perovskite photodetectors, providing a pathway toward scalable, high performance imaging systems beyond conventional silicon-based technologies.\u0000 </div>","PeriodicalId":229,"journal":{"name":"Small Methods","volume":"10 19","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148429509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solid-Liquid Interdiffusion Bonding Enabling Heterogeneous Integration on Flexible and Shapeable Polymer Platforms. 在柔性和可塑聚合物平台上实现非均质集成的固液互扩散键合。

IF 8.7 2区材料科学

Small Methods Pub Date : 2026-10-06 DOI: 10.1002/smtd.71031

Yeji Lee, Vineeth Kumar Bandari, Daniil Karnaushenko, Dmitriy D Karnaushenko, Thomas Blaudeck, Chongxian Wang, Uwe Zschenderlein, Nathanael Jöhrmann, Bernhard Wunderle, Oliver G Schmidt

{"title":"Solid-Liquid Interdiffusion Bonding Enabling Heterogeneous Integration on Flexible and Shapeable Polymer Platforms.","authors":"Yeji Lee, Vineeth Kumar Bandari, Daniil Karnaushenko, Dmitriy D Karnaushenko, Thomas Blaudeck, Chongxian Wang, Uwe Zschenderlein, Nathanael Jöhrmann, Bernhard Wunderle, Oliver G Schmidt","doi":"10.1002/smtd.71031","DOIUrl":"https://doi.org/10.1002/smtd.71031","url":null,"abstract":"The integration of rigid microscale electronics onto soft and flexible polymer substrates remains a key challenge for flexible electronics, biointegrated systems, and body-first microrobotics, where the mechanical body, material architecture, morphology, and primary functions are designed first, while the electronic processing unit (e.g., a Si CMOS chiplet), acting as the brain to control the robot´s functionalities, is integrated later. Existing approaches, including anisotropically conductive adhesives and transfer printing, rely on non-patternable or particle-based interconnects, thick bonding layers, or complex processing, constraining interconnect density, scalability, and compatibility with mechanical transformations such as bending, rolling, or folding. Here, we present a patterned solid-liquid interdiffusion (SLID) bonding approach enabling deterministic, fine-pitch heterogeneous integration on ultrathin polymer substrates compatible with standard microfabrication. Electroplated Cu/Sn pillar bumps fabricated on 5 µm polyimide are systematically characterized to define geometric, electrical, and mechanical process window for reliable SLID bonding. The resulting interconnects exhibit controlled solder reflow, low electrical resistance, high mechanical robustness, and minimum inter-pillar spacings of 10 µm without adhesives or conductive particles. Microscale light-emitting diodes bonded by SLID remain functional during self-rolling, bending and folding-driven three-dimensional assembly with integrated silicon microchips. This work provides a scalable framework for mechanically reconfigurable microrobotic and flexible microsystems.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e71031"},"PeriodicalIF":8.7,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149101555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding Peptide Conformational Ensembles at Gold Interfaces for Sequence-Encoded Bio-Interface Engineering. 在金界面上解码肽构象集成的序列编码生物界面工程。

IF 8.7 2区材料科学

Small Methods Pub Date : 2026-10-06 DOI: 10.1002/smtd.71094

Jinhua He, Hao Ma, Xiaohan Xi, Haofei Geng, Qingling Zhou, Yaxiang Wang, Sen Yan, Lei Zhang, Jiayi Wang, Mingyu Chen, Enhui Ma, Xiang Wang, Bin Ren

{"title":"Decoding Peptide Conformational Ensembles at Gold Interfaces for Sequence-Encoded Bio-Interface Engineering.","authors":"Jinhua He, Hao Ma, Xiaohan Xi, Haofei Geng, Qingling Zhou, Yaxiang Wang, Sen Yan, Lei Zhang, Jiayi Wang, Mingyu Chen, Enhui Ma, Xiang Wang, Bin Ren","doi":"10.1002/smtd.71094","DOIUrl":"https://doi.org/10.1002/smtd.71094","url":null,"abstract":"Understanding how biomolecules organize at metal interfaces is of central importance for a wide range of sensing and biointerfacial technologies, yet it is still challenging to decipher their microscopic organization because conventional macroscopic measurements cannot reveal the hidden assembly states of flexible adsorbates. Here, a multiscale analytical strategy integrates electrochemical active surface area (ECSA), gap-mode surface-enhanced Raman spectroscopy (SERS), and ensemble-based all-atom molecular dynamics simulations to connect macroscopic adsorption behavior with microscopic assembly states on gold surfaces. Tyr-Tyr-Tyr (YYY), an extraordinarily adhesive peptide with an unresolved mechanism for its strong interfacial affinity, is used as a model system. The results show that fundamentally distinct microscopic organizations can exhibit nearly identical macroscopic adsorption states. Systematic sequence mutants further reveal that consecutive Tyr residues sustain the hydrogen-bond-driven lateral aggregation, from which Tyr-Phe-Tyr (YFY) emerges as a compact anchoring motif that retains strong gold affinity while suppressing aggregation. Beyond resolving this peptide-gold assembly problem, our work advances an ensemble-based perspective for interpreting interfacial Raman spectra and provides a foundation for programmable bio-interface engineering, with potential applications in biosensing, peptide/protein arrays, and other functional bio-metal platforms.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e71094"},"PeriodicalIF":8.7,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149101433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Microbe Transcriptomics Reveal Functional Heterogeneity in Sediment Microbiomes. 单微生物转录组学揭示沉积物微生物组的功能异质性。

IF 8.7 2区材料科学

Small Methods Pub Date : 2026-10-06 DOI: 10.1002/smtd.71082

Lingxiao Zhu, Xiao Xiong, Quantao Zhang, Wenjie Cai, Yongcheng Wang

{"title":"Single-Microbe Transcriptomics Reveal Functional Heterogeneity in Sediment Microbiomes.","authors":"Lingxiao Zhu, Xiao Xiong, Quantao Zhang, Wenjie Cai, Yongcheng Wang","doi":"10.1002/smtd.71082","DOIUrl":"https://doi.org/10.1002/smtd.71082","url":null,"abstract":"Sediment microbiomes drive global biogeochemical cycling, but their functional heterogeneity and transcriptional activity are hardly resolved at single-cell resolution. We optimized a scalable single-microbe RNA sequencing workflow for environmental muddy sediment samples, achieving high-throughput single cell sequencing across environmental gradients. The method integrates iohexol-based microbial enrichment, optimized enzymatic digestion step, droplet-based single-cell barcoding, and RNA sequencing, then generating a transcriptomic atlas of 55 859 high-quality microbial cells from 8 composite samples covering freshwater, estuarine and nearshore marine habitats. Comparison with metagenomic data showed a high consistency in species composition, supporting the reliability of single-microbe RNA sequencing in community profiling. Taxonomic analysis revealed site-specific microbial communities associated with methane oxidation, sulfur cycling and anaerobic nitrogen metabolism. 15 distinct functional clusters were identified transcriptionally, showing functional heterogeneity within sedimentary microbiome. Co-expression network analysis further resolved coordinated gene modules, with module activities varying across samples. Overall, this work establishes a practical framework for single-microbe transcriptomics in sediment systems and demonstrates the potential of single-cell resolution to uncover functional heterogeneity within complex microbial communities.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e71082"},"PeriodicalIF":8.7,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149101515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthetic Methodologies and Structural Engineering of Biomass-Derived Adsorbents for CO₂ Capture. 生物质吸附剂捕获二氧化碳的合成方法和结构工程。

IF 8.7 2区材料科学

Small Methods Pub Date : 2026-10-06 DOI: 10.1002/smtd.71059

Song-Chan Pak, Fengyu Gao, Myong-Hyok Paek, Yifan Wang, Yaxin Niu, Kai Chen, Yuansong Zhou, Shunzheng Zhao, Xiaolong Tang, Honghong Yi

{"title":"Synthetic Methodologies and Structural Engineering of Biomass-Derived Adsorbents for CO2 Capture.","authors":"Song-Chan Pak, Fengyu Gao, Myong-Hyok Paek, Yifan Wang, Yaxin Niu, Kai Chen, Yuansong Zhou, Shunzheng Zhao, Xiaolong Tang, Honghong Yi","doi":"10.1002/smtd.71059","DOIUrl":"https://doi.org/10.1002/smtd.71059","url":null,"abstract":"Developing sustainable, scalable, low-cost adsorbents constitutes a core prerequisite to realize practically viable CO2 capture technologies. Biomass-derived porous carbons represent attractive candidate sorbents owing to renewable raw feedstocks, tailorable pore architecture, intrinsic hydrophobic character, and moderate regeneration energy demand. This review adopts a methodology- and engineering-driven perspective to examine rational design principles, scalable fabrication routes, and structural engineering strategies of biomass-derived adsorbents for high-efficiency CO2 capture. Material-level links between manufacturing pathways, physicochemical features, and resultant CO2 adsorption performance are critically analyzed, covering a broad suite of carbonization, activation, and surface-modification approaches. Beyond material characteristics, engineering-relevant aspects are addressed, including pelletization of powdered sorbents, reactor configuration, and practical implementation within PSA (pressure swing adsorption), VSA (vacuum swing adsorption), and TSA (temperature swing adsorption) cyclic adsorption workflows for industrial-scale deployment. Key existing bottlenecks are outlined, such as lack of standardized synthesis workflows, insufficient anti-contamination performance, barriers for large-scale manufacturing, and absence of unified performance-assessment benchmarks. Corresponding future research avenues are suggested to promote the translation of lab-scale biomass-derived adsorbent research toward real-world CO2-capture practice.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e71059"},"PeriodicalIF":8.7,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149101739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Robust Reconstruction of Atomic Force Microscopy Tip Shape From Force-Indentation Curves. 基于力压痕曲线的原子力显微镜尖端形状鲁棒重建。

IF 8.7 2区材料科学

Small Methods Pub Date : 2026-10-06 DOI: 10.1002/smtd.71081

Logan J Kirsch, Jason P Killgore, Gregory J Rodin, Loukas F Kallivokas, Filippo Mangolini

引用次数: 0

Effect of MXene Surface Structures on the Absorption Component in Reflection-Dominated Electromagnetic Interference Shielding. MXene表面结构对反射主导电磁干扰屏蔽中吸收分量的影响。

IF 8.7 2区材料科学

Small Methods Pub Date : 2026-10-06 DOI: 10.1002/smtd.71084

Yuecheng Lai, Yuanyuan Tan, Chengkai Bao, Shuming Zhou, Guang Mo, Yunpeng Liu, Xueqing Xing, Zhongjun Chen, Yifang Song, Ke Li, Mengmeng Wang, Haiying Wang, Lanhong Dai, Zhonghua Wu

{"title":"Effect of MXene Surface Structures on the Absorption Component in Reflection-Dominated Electromagnetic Interference Shielding.","authors":"Yuecheng Lai, Yuanyuan Tan, Chengkai Bao, Shuming Zhou, Guang Mo, Yunpeng Liu, Xueqing Xing, Zhongjun Chen, Yifang Song, Ke Li, Mengmeng Wang, Haiying Wang, Lanhong Dai, Zhonghua Wu","doi":"10.1002/smtd.71084","DOIUrl":"https://doi.org/10.1002/smtd.71084","url":null,"abstract":"MXene nanoflakes have attracted significant attention due to their outstanding electromagnetic interference (EMI) shielding effectiveness (SE). However, understanding the relationship between shielding performance and complex surface microstructures of MXene nanoflakes remains challenging. In this study, flexible selfstanding Ti3C2Tx films were synthesized under various preparation conditions, achieving an ultrahigh EMI shielding effectiveness exceeding 70 dB. The morphology and microstructure were characterized using scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HRTEM), synchrotron radiation X-ray diffraction (SR-XRD), X-ray absorption fine structure spectroscopy (XAFS), and X-ray photoelectron spectroscopy (XPS). Based on the layered structure of MXene and the nearest- and next-nearest-neighbor coordination numbers around Ti atoms, an XAFS-based model was developed to estimate the surface Ti-vacancy rate (p) and average surface Tx-termination number (m). The results reveal that the shielding performance is jointly influenced by p and m through nonlinear coupling, with m exerting a more pronounced influence. Furthermore, the correlations among EMI absorption shielding performance, electrical conductivity, attenuation constant, surface structural parameters, and synthesis conditions were systematically analyzed, and the mechanism by which the surface structures affect the absorption shielding effectiveness was briefly discussed. We anticipate that this work will contribute to the surface engineering and optimization of advanced MXene-based shielding materials.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e71084"},"PeriodicalIF":8.7,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149101510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stage-Controlled Synthesis of MXene/MAX Heterostructures With Local Atomic Disorder for Enhanced Lithium-Ion Storage. 阶段控制合成具有局部原子无序的MXene/MAX异质结构以增强锂离子存储。

IF 8.7 2区材料科学

Small Methods Pub Date : 2026-10-06 DOI: 10.1002/smtd.71093

Xiaoyu Han, Kefu Zhu, Jialin Shi, Wei Jiang, Shiqiang Wei, Lunhua He, Li Song

{"title":"Stage-Controlled Synthesis of MXene/MAX Heterostructures With Local Atomic Disorder for Enhanced Lithium-Ion Storage.","authors":"Xiaoyu Han, Kefu Zhu, Jialin Shi, Wei Jiang, Shiqiang Wei, Lunhua He, Li Song","doi":"10.1002/smtd.71093","DOIUrl":"https://doi.org/10.1002/smtd.71093","url":null,"abstract":"Molten-salt etching has emerged as an effective and environmentally friendly strategy for converting MAX phases into MXene, while also offering opportunities to access novel intermediate heterostructures through precise process control. However, previous studies have mainly focused on the complete transformation from MAX phases to MXene, whereas the controllable synthesis and structural elucidation of intermediate products remain largely unexplored. Here, by tuning the etching time in a CuCl2-based molten-salt system, controllable structural evolution from Nb2GaC to Nb2CTx is achieved. A series of intermediate products, denoted as Nb2GaC-n, are successfully isolated. With increasing etching time, the interlayer Cu and Ga contents gradually decrease, enabling precise tuning of the interlayer atomic configuration. Synchrotron x-ray absorption fine structure spectroscopy reveals that the controlled etching process induces electronic-structure modulation and short-range structural disorder. Benefiting from the optimized local atomic environment, the intermediate heterostructure delivers enhanced lithium-storage performance, including a high reversible capacity of 281 mAh g-1 at 0.1 A g-1 and remarkable cycling stability, with 177 mAh g-1 retained after 2000 cycles at 1.0 A g-1. This work establishes a viable strategy for engineering MXene-based heterostructures with tailored local atomic configurations.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e71093"},"PeriodicalIF":8.7,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149101637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical Photothermal Infrared Signatures Distinguish Organelle Identity and Health in Single Cells. 光学光热红外特征识别单细胞细胞器身份和健康。

IF 8.7 2区材料科学

Small Methods Pub Date : 2026-10-05 DOI: 10.1002/smtd.71080

Michael J Burke, Victor S Batista, Caitlin M Davis

{"title":"Optical Photothermal Infrared Signatures Distinguish Organelle Identity and Health in Single Cells.","authors":"Michael J Burke, Victor S Batista, Caitlin M Davis","doi":"10.1002/smtd.71080","DOIUrl":"https://doi.org/10.1002/smtd.71080","url":null,"abstract":"Cells maintain homeostasis by dynamically reorganizing their organelles to tune metabolism in response to stress. Fluorescence microscopy maps organelle structures with subcellular resolution but provides limited chemical information. Infrared (IR) imaging offers a label-free alternative for probing intrinsic molecular vibrations that report on lipids, carbohydrates, and nucleic acids. Here, we combine submicron optical photothermal IR imaging with machine learning to classify subcellular structures in fixed U-2 OS cells in a cell state dependent manner. Using fluorescent-labeled organelles as ground truth, we trained and evaluated random forest (RF) classifiers and U-Net convolutional neural networks to identify organelles from IR spectra in healthy cells. The classifiers accurately identified multiple organelles, including the endoplasmic reticulum, Golgi apparatus, mitochondria, nucleus, nucleolus, and stress granules. Classifiers trained in U-2 OS cells transferred without retraining to HEK 293 cells, consistent with conserved organelle biochemical composition across cell types. Conversely, the classifiers failed under cellular stress which indicates sensitivity to stress-induced changes in organelle state. Together, these results establish a scalable, label-free strategy for high-resolution mapping of organelle biochemical composition and provide a foundation for subcellular biomarker discovery and disease-state diagnostics.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e71080"},"PeriodicalIF":8.7,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149101564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prediction of Oxidative Stress- and Anticancer Drug-Induced Cellular Senescence Using Holotomography and Interpretable Attention-Based Contrastive Learning. 利用全息断层扫描和可解释的基于注意的对比学习预测氧化应激和抗癌药物诱导的细胞衰老。

IF 8.7 2区材料科学

Small Methods Pub Date : 2026-10-05 DOI: 10.1002/smtd.71091

Avinash Yaganapu, Amarnath Singam, Sai Phani Parsa, Calvinique Lee, Jennifer Zheng, Sunjeet Saha, Jeong Hee Kim, Seungman Park, Mingon Kang

{"title":"Prediction of Oxidative Stress- and Anticancer Drug-Induced Cellular Senescence Using Holotomography and Interpretable Attention-Based Contrastive Learning.","authors":"Avinash Yaganapu, Amarnath Singam, Sai Phani Parsa, Calvinique Lee, Jennifer Zheng, Sunjeet Saha, Jeong Hee Kim, Seungman Park, Mingon Kang","doi":"10.1002/smtd.71091","DOIUrl":"https://doi.org/10.1002/smtd.71091","url":null,"abstract":"Quantitative assessment of the complex morphological and structural changes of cells and organelles during senescence is critical for understanding the level of senescence, which is a key step toward developing anti-aging therapeutics and preventing aging-related diseases. Despite its importance, a robust quantitative method for evaluating cellular senescence is still lacking. Recent advances in holotomography (HT), a label-free imaging technique, address these challenges by enabling high-contrast cell imaging with subcellular specificity, which enables accurate detection and characterization of senescent cells, without the constraints of fluorescence-based imaging. In this study, we introduce an artificial intelligence (AI)-powered Biologically Interpretable Contrastive and Attention-based Network integrated with HT, called BICAN-HT, which leverages high-resolution images to analyze nucleus-cytoplasm interactions for biologically interpretable and quantitative assessment of cellular senescence with enhanced predictive power. We conducted in-depth experiments inducing cellular senescence using three stressors, including hydrogen peroxide (H2O2), doxorubicin (Dox), and palbociclib (Pal), across two different cell lines. BICAN-HT consistently outperformed current state-of-the-art models across multiple stress conditions and cell types, while capturing the morphological features and spatial relationships of cells and organelles within the nucleus and cytoplasm during senescence. BICAN-HT has the potential to transform aging research and accelerate the development of anti-aging interventions.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e71091"},"PeriodicalIF":8.7,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149101530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0