Small Methods最新文献_第6页

Filter-Assisted ICP-MS Tumor Liquid Biopsy Enabled by Dual-Target-Regulated Functional DNA Nanospheres Cascade Amplification. 双靶标调控的功能DNA纳米球级联扩增实现过滤器辅助ICP-MS肿瘤液体活检。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-03-22 DOI: 10.1002/smtd.202500314

Yiman Wu, Yue Wang, Li Yan, Ke Huang, Kai Chang, Piaopiao Chen

{"title":"Filter-Assisted ICP-MS Tumor Liquid Biopsy Enabled by Dual-Target-Regulated Functional DNA Nanospheres Cascade Amplification.","authors":"Yiman Wu, Yue Wang, Li Yan, Ke Huang, Kai Chang, Piaopiao Chen","doi":"10.1002/smtd.202500314","DOIUrl":"https://doi.org/10.1002/smtd.202500314","url":null,"abstract":"An ultrasensitive ICP-MS aptasensor is developed utilizing a label-free, simple filter membrane-assisted separation technique combined with nucleic acid signal amplification for the analysis of circulating tumor cells (CTCs) in lung cancer clinical samples. The approach is based on the high-affinity interaction between aptamers and PD-L1 and mucin 1, which are overexpressed on the cell surface, in conjunction with functional Y-DNA nanospheres and catalytic hairpin assembly amplifications, enabling the simultaneous detection of two proteins. Additionally, a four-armed nanostructure with significant spatial site resistance is self-assembled by introducing streptavidin with biotinylated-hairpin structures, improving the separation efficiency of the filter membrane. This structural design enables the effective isolation of biotin-T-Hg2+-T and biotin-C-Ag+-C from free Hg2+ and Ag+, facilitating highly sensitive dual-protein detection via ICP-MS. The limits of detection reached ag mL-1 levels for proteins and single-cell levels for A549 cells. CTCs are extracted from whole blood samples of lung cancer patients within 45 min through a simple centrifugation procedure. Quantification of CTCs is performed in 37 clinical samples, demonstrating results consistent with clinical diagnoses. The assay exhibits a specificity of 100% and a sensitivity of 94.5%.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500314"},"PeriodicalIF":10.7,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143676669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced Fabrication of Ultrathin Ruthenium Films Using Synergistic Atomic Layer Deposition and Etching. 利用协同原子层沉积和蚀刻技术制备超薄钌薄膜。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-03-21 DOI: 10.1002/smtd.202402166

Jeongbin Lee, Jung-Tae Kim, Jieun Oh, Dongjun Lee, Seo-Hyun Lee, Hyekyung Kim, Jiwoo Oh, Younseon Wang, Woo-Hee Kim

{"title":"Advanced Fabrication of Ultrathin Ruthenium Films Using Synergistic Atomic Layer Deposition and Etching.","authors":"Jeongbin Lee, Jung-Tae Kim, Jieun Oh, Dongjun Lee, Seo-Hyun Lee, Hyekyung Kim, Jiwoo Oh, Younseon Wang, Woo-Hee Kim","doi":"10.1002/smtd.202402166","DOIUrl":"https://doi.org/10.1002/smtd.202402166","url":null,"abstract":"Atomic-level surface preparation, using additive and subtractive atomic layer processes, has gradually become crucial for the more active process variations and highly selective process requirements. Precise control of surface roughness and coverage is a critical consideration in the fabrication of metal thin films. Herein, the fabrication of ultrathin, smooth Ru films with a thickness reduced to below 3 nm is reported. This process involves etching back after depositing a thick Ru film using a synergistic combination of atomic layer deposition (ALD) and atomic layer etching (ALE) techniques. The surface smoothing effect, while preserving surface coverage, is validated by initially performing the ALD process for Ru with (ethylbenzyl)(1-ethyl-1,4-cyclohexadienyl)Ru(0) precursor and O2 gas, followed by the ALE process with 2,4-pentanedione and O2 radicals. Under optimized conditions for atomically flat Ru surfaces, the surface quality of Ru films processed by ALD, and the combined ALD/ALE methods are compared. Consequently, it is demonstrated for the first time that the combined ALD/ALE process effectively reduces both thickness and asperities while smoothing the surface and maintaining nearly complete surface coverage down to the ≈1 nm scale. This approach enables the production of advanced electronic devices with precise control over surface properties at the Ångström level.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2402166"},"PeriodicalIF":10.7,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143672963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Fused Diketopyrrolopyrrole Derivative for Single-Crystal Visible-NIR Organic Phototransistor. 单晶可见光-近红外有机光电晶体管的熔融双酮吡咯衍生物。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-03-21 DOI: 10.1002/smtd.202402250

Tianqiang Cui, Nan Luo, Zhilei Zhang, Jie Liu, Yanan Sun, Dawei Liu, Yamin Zhang, Xiangfeng Shao, Hao-Li Zhang, Lang Jiang, Zitong Liu

{"title":"A Fused Diketopyrrolopyrrole Derivative for Single-Crystal Visible-NIR Organic Phototransistor.","authors":"Tianqiang Cui, Nan Luo, Zhilei Zhang, Jie Liu, Yanan Sun, Dawei Liu, Yamin Zhang, Xiangfeng Shao, Hao-Li Zhang, Lang Jiang, Zitong Liu","doi":"10.1002/smtd.202402250","DOIUrl":"https://doi.org/10.1002/smtd.202402250","url":null,"abstract":"Organic phototransistors (OPTs) have garnered significant attention due to their potential in wearable and flexible electronics. However, achieving high carrier mobility and broadband response in organic semiconductors for OPTs remains a challenge. In this work, a new fused diketopyrrolopyrrole (FDPP) derivative is reported, 2,9-bis(4-hexylphenyl)-7H,14H-thieno[3',2':7,8]indolizino[2,1-a]thieno[3,2-g]indolizine-7,14-dione (FDPP-p-C6), synthesized through N-cyclization of DPP with an adjacent thiophene unit. This N-cyclization ensures backbone planarity, while the hexyl side chains remain distant from the fused core, minimizing steric hindrance and promoting efficient intermolecular stacking. Consequently, single crystals of FDPP-p-C6 exhibit a planar backbone and a typical herringbone packing arrangement, facilitating charge transport. The single-crystal organic field-effect transistors (OFETs) demonstrate p-type charge transport, achieving maximum mobility of 0.20 cm2 V-¹ s-¹. Additionally, the single-crystal OPTs show promising performance, with high responsivity across a broad spectral range and a photoresponsivity of 2.2 × 103 A W-¹ along with a specific detectivity, derived from the noise current, of 2.8 × 1010 Jones. This study highlights the potential of FDPP as a key material for advancing single-crystal OFET and OPT technologies, propelling relevant research forward.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2402250"},"PeriodicalIF":10.7,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143672942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral Delivery Strategies for Biological Drugs. 生物药物口服给药策略。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-03-21 DOI: 10.1002/smtd.202401355

Huiling Zeng, Mingyang Ma, Xiuju Peng, Yangyang Xie, Angel Xie, Bo Deng, Jiang Ouyang, Wei Tao, Pei Yang, Wei He

{"title":"Oral Delivery Strategies for Biological Drugs.","authors":"Huiling Zeng, Mingyang Ma, Xiuju Peng, Yangyang Xie, Angel Xie, Bo Deng, Jiang Ouyang, Wei Tao, Pei Yang, Wei He","doi":"10.1002/smtd.202401355","DOIUrl":"https://doi.org/10.1002/smtd.202401355","url":null,"abstract":"Biologics have low toxicity and are highly specific and biocompatible, offering advantages over small-molecule drugs. The administration of biologics in oral form provides a significant benefit in improving patient compliance. However, oral administration faces the challenge of a harsh gastrointestinal environment, including low pH, enzyme degradation, and poor intestinal epithelium permeability, which limits the bioavailability of biologics. As a result, the administration of biologics remains primarily in the parenteral form. This review introduces the physiological barriers encountered by oral biologics delivery, describes the oral biologics currently on the market or under clinical trials, as well as oral biologics-based technologies, and discusses the recent progress on novel oral delivery technologies such as nanoparticle-delivery systems, ionic liquids, and microneedles. Specifically, colon-targeted approaches for oral biologics delivery are also explored, as the colon could be a more optimal absorption site due to having less diverse proteolytic enzymes and relatively limited digestibility compared to the upper gastrointestinal tract (GIT). Lastly, the future research directions for oral biologics are highlighted and it is concluded that with an in-depth study of biological drugs and advancement in delivery methods, oral biologics can pioneer new opportunities.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2401355"},"PeriodicalIF":10.7,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

H-Embedding Induced Electron Localization in Pd Lattice for Improving Electrochemical Hydrogen Purification. 包氢诱导Pd晶格中的电子定位改善电化学氢净化。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-03-21 DOI: 10.1002/smtd.202500249

Xuanwei Yin, Cong Wei, Chongyang Tang, Zenan Bian, Bo Liu, Xinqiang Wang, Yaxiong Yang, Yanyan Fang, Hongge Pan, Gongming Wang

{"title":"H-Embedding Induced Electron Localization in Pd Lattice for Improving Electrochemical Hydrogen Purification.","authors":"Xuanwei Yin, Cong Wei, Chongyang Tang, Zenan Bian, Bo Liu, Xinqiang Wang, Yaxiong Yang, Yanyan Fang, Hongge Pan, Gongming Wang","doi":"10.1002/smtd.202500249","DOIUrl":"https://doi.org/10.1002/smtd.202500249","url":null,"abstract":"Electrochemical hydrogen purification (EHP) technology with high-efficiency and easy-operation holds great potential in blended hydrogen transportation, which is currently restricted to proton exchange membrane system and Pt-based catalysts. As promising candidates used in alkaline anion exchange membrane system, Pd-based catalysts are hampered by the intense interaction between H* and delocalized 4d electrons, resulting in unsatisfactory catalytic activity. In this study, a marked enhancement of the alkaline membrane-based EHP performance is achieved, with hydrogen purity up to 99.96% separated from a CH4-H2 mixture, by strategically incorporating interstitial H atoms into Pd lattices for improving the anodic hydrogen oxidation reaction. Detailed characterizations and density functional theory calculations elucidate that the presence of interstitial H localizes free electrons into Pd-H covalent bonds, thereby weakening the interaction between surface-adsorbed H* and the catalytic surface. Moreover, operando spectroscopies and ab initio molecular dynamic simulations reveal that the enhanced interaction between the catalyst surface and interfacial water by electron delocalization, facilitates the desorption of H* to the interfacial water layer during catalysis. This research highlights the pivotal role of electronic localization in modulating the adsorption strength of key reaction intermediates for the design of efficient Pd-based catalysts.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500249"},"PeriodicalIF":10.7,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Challenges and Opportunities of Uranium Extraction From Seawater: a Systematic Roadmap From Laboratory to Industry (Small Methods 3/2025) 海水提铀的挑战与机遇：从实验室到工业的系统路线图（小方法3/2025）

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-03-20 DOI: 10.1002/smtd.202570020

Tao Wang, Binbin Tao, Bin Zuo, Guoze Yan, Shaoqing Liu, Ruoyu Wang, Zhongzhou Zhao, Feifei Chu, Zhengtong Li, Yusuke Yamauchi, Xingtao Xu

引用次数: 0

Strain-Engineered 2D Materials: Challenges, Opportunities, and Future Perspectives (Small Methods 3/2025) 应变工程二维材料：挑战、机遇和未来展望（Small Methods 3/2025）

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-03-20 DOI: 10.1002/smtd.202570018

Ajit Kumar Katiyar, Jong-Hyun Ahn

引用次数: 0

Self-Powered Hydrogen Production via Laser-Coordinated NiCoPt Alloy Catalysts in an Integrated Zn-Hydrazine Battery with Hydrazine Splitting (Small Methods 3/2025) 基于激光协调NiCoPt合金催化剂的一体化锌-肼电池自供电制氢（小型方法3/2025）

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-03-20 DOI: 10.1002/smtd.202570016

Akash Prabhu Sundar Rajan, Raja Arumugam Senthil, Cheol Joo Moon, Anuj Kumar, Ahreum Min, Mohd Ubaidullah, Myong Yong Choi

引用次数: 0

Integrating Metabolic RNA Labeling-Based Time-Resolved Single-Cell RNA Sequencing with Spatial Transcriptomics for Spatiotemporal Transcriptomic Analysis (Small Methods 3/2025) 整合基于代谢RNA标记的时间分辨单细胞RNA测序与空间转录组学进行时空转录组学分析（Small Methods 3/2025）

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-03-20 DOI: 10.1002/smtd.202570019

Xiaoyong Chen, Shichao Lin, Honghai You, Jinyuan Chen, Qiaoyi Wu, Kun Yin, Fanghe Lin, Yingkun Zhang, Jia Song, Chenyu Ding, Dezhi Kang, Chaoyong Yang

引用次数: 0

Neutrophil Hitchhiking-Mediated Delivery of ROS-Scavenging Biomimetic Nanoparticles for Enhanced Treatment of Atherosclerosis. 中性粒细胞搭便车介导的ros清除仿生纳米颗粒增强动脉粥样硬化治疗。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-03-20 DOI: 10.1002/smtd.202402019

Ming Wu, Mengjuan Chen, Yuzhen Zhao, Xijun Zhang, Xiao Ding, Jianjun Yuan, Jinjin Shi, Wenyan Yu, Haohui Zhu

{"title":"Neutrophil Hitchhiking-Mediated Delivery of ROS-Scavenging Biomimetic Nanoparticles for Enhanced Treatment of Atherosclerosis.","authors":"Ming Wu, Mengjuan Chen, Yuzhen Zhao, Xijun Zhang, Xiao Ding, Jianjun Yuan, Jinjin Shi, Wenyan Yu, Haohui Zhu","doi":"10.1002/smtd.202402019","DOIUrl":"https://doi.org/10.1002/smtd.202402019","url":null,"abstract":"Atherosclerosis (AS), a chronic inflammatory disease and a leading cause of cardiovascular morbidity and mortality worldwide, is a significant contributor to disability. Neutrophil extracellular traps (NETs) have been closely associated with the progression of AS and plaque vulnerability. However, developing a treatment strategy that specifically targets neutrophils and effectively reduces NET release at the lesion site remains a major challenge. In this study, a biomimetic nanosystem with neutrophil-targeting properties is engineered. Coating Prussian blue nanoparticles with bacterial biomimetic membranes (MPB NPs) enables specific recognition and internalization by neutrophils. By hitching onto neutrophils, the MPB NPs scavenge intracellular reactive oxygen species (ROS) and suppress NET formation at the lesion site. Importantly, MPB NPs reduce the size of atherosclerotic plaques by 3.29-fold, from 22.53% to 6.85%, stabilize the plaques, and halt their progression in atherosclerotic mouse models. These findings suggest that MPB NPs offer a promising therapeutic strategy for atherosclerosis, and provide a versatile platform for the treatment of NET-associated diseases.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2402019"},"PeriodicalIF":10.7,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143661865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0