Small MethodsPub Date : 2025-06-05DOI: 10.1002/smtd.202500662
Huaqing Du, Weipeng Chen, Xiang-Yu Kong, Liping Wen
{"title":"2D Carbon Material-Based Bio-Abiotic Interfaces.","authors":"Huaqing Du, Weipeng Chen, Xiang-Yu Kong, Liping Wen","doi":"10.1002/smtd.202500662","DOIUrl":"https://doi.org/10.1002/smtd.202500662","url":null,"abstract":"<p><p>2D carbon materials emerge as transformative platforms for bio-abiotic interface engineering, leveraging their distinctive physicochemical properties - including exceptional surface-to-volume ratios, outstanding mechanical strength, tunable electrical conductivity, and inherent biocompatibility. These attributes collectively enable breakthrough innovations across biosensing architectures, targeted therapeutic delivery systems, biomimetic tissue scaffolds, and electrophysiological modulation technologies. Contemporary research yields significant advances in synthesis and fabrication techniques for 2D carbon materials. The flexibility of these materials ensures compatibility with dynamic tissues, while their surface modifiability allows functionalization for specific applications. Emerging implementations span three critical domains of modern bioengineering: implantable devices, wearable devices, and human-machine interfaces. This review summarizes fabrication strategies correlating nanoscale architectures with the macroscopic performance of 2D carbon materials and their applications across the three fields. By establishing critical design principles and addressing current challenges in the transformation of scientific and technological achievements, this work aims to provide a foundational framework for developing next-generation intelligent bio-abiotic interface systems with enhanced functionality and biological fidelity.</p>","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500662"},"PeriodicalIF":10.7,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144232812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Small MethodsPub Date : 2025-06-05DOI: 10.1002/smtd.202500448
So Young Choi, Jieun Kim, Eun Ho Song, Eunhye Park, Jingrui Wu, Juyeon Yoo, Jwa-Min Nam
{"title":"Design, Mechanisms, and Applications of DNA-Mediated Dynamically Reconfigurable Plasmonic Gold Nanostructures.","authors":"So Young Choi, Jieun Kim, Eun Ho Song, Eunhye Park, Jingrui Wu, Juyeon Yoo, Jwa-Min Nam","doi":"10.1002/smtd.202500448","DOIUrl":"https://doi.org/10.1002/smtd.202500448","url":null,"abstract":"<p><p>Unlike the fixed formations of static gold nanostructures (AuNSs), reconfigurable AuNSs offer versatility when designing nanomaterials and biosensors because they can dynamically respond to external stimuli in a tunable manner. These dynamic systems enable in situ reaction monitoring and cyclic switching functions. An appropriate design of the architecture of the nanostructures is crucial because it dictates the operational principles of the system. This review explores the design and working principles of reconfigurable gold-based plasmonic nanostructures by modulating highly programmable DNA and through chemical and physical stimuli such as temperature, light, pH, and metal ions. Methods are discussed to control these factors and use them as actuation handles for affecting the reconfigurability of these structures. In addition, the utilization of these properties and functions in bio-applications and functional hybrid materials is illustrated, which demonstrates the practical applications of reconfigurable AuNSs in advanced materials science and biomedicine.</p>","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500448"},"PeriodicalIF":10.7,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144232814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Collapsing Carbon Nanotube Enhances Its Phonon Transport.","authors":"Taocheng Yu, Md Azimul Haque, Derya Baran, Hanying Li, Wee-Liat Ong","doi":"10.1002/smtd.202401662","DOIUrl":"https://doi.org/10.1002/smtd.202401662","url":null,"abstract":"<p><p>Carbon nanotubes (CNTs) radially deform when they interact with the surrounding matrix in heterostructures or metal electrodes in electronic devices, affecting their electrical properties. As thermal management becomes increasingly important for high-performance CNT-based nanoelectronics, understanding how such deformations affect the thermal conductivity (κ) of CNT-based devices has emerging significance. The investigation shows that the CNT's radially malleable nature enables the CNT to collapse, allowing atoms across the circumference to couple directly and enhance its thermal transport. Through solving the phonon Boltzmann transport equation at 300 K, the κ of a long (6,6) CNT increases up to six times upon radial compression to 18 GPa. The carbon-carbon bonds become stretched but the acoustic and optical phonons of non-longitudinal polarizations are surprisingly stiffened. This stiffening weakens the anharmonicity, leading to an increase in the phonon relaxation time and κ. However, for CNTs shorter than 10<sup>3</sup> nm, a peak in κ occurs with increasing stress. This peak is produced as the increased phonon-boundary scatterings in shorter CNTs offset the increased phonon relaxation time at high stress. Hence, an optimal stress level can increase the κ of CNTs, optimizing the performances of radially-deformed CNT heterostructures.</p>","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2401662"},"PeriodicalIF":10.7,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Small MethodsPub Date : 2025-06-04DOI: 10.1002/smtd.202500178
Yang Bai, Jie Qu, Shuxuan Li, Juntao Zhou, Zhonghui Shen, Xin Zhang
{"title":"Force-Driven In Situ Growth of Nanostructured Silica Fillers in Polyetherimide Nanocomposites.","authors":"Yang Bai, Jie Qu, Shuxuan Li, Juntao Zhou, Zhonghui Shen, Xin Zhang","doi":"10.1002/smtd.202500178","DOIUrl":"https://doi.org/10.1002/smtd.202500178","url":null,"abstract":"<p><p>Inorganic nanofillers have been extensively employed to tailor or enhance the properties of polymer nanocomposites, wherein the morphology of the nanofillers plays a pivotal role. Here, the use of a directional force is demonstrated to drive the in situ growth of inorganic silica nanofillers with controllable morphologies, including silica spherical nanoparticles, nanofibers, and nanosheets, within the polyetherimide (PEI) nanocomposites. The in situ grown nanofillers exhibit exceptional interfacial compatibility and uniform dispersion in the PEI matrix. Furthermore, the high aspect ratios of the silica nanofillers, combined with the interfacial effects, confer significantly enhanced mechanical strength, thermal stability, and electrical insulation upon the PEI nanocomposites, thereby unlocking substantial potential for capacitive energy storage applications. This is evidenced by the attainment of an ultrahigh dielectric energy density of 8.9 J cm<sup>-3</sup> (150 °C) and 5.6 J cm<sup>-3</sup> (200 °C) with an energy efficiency exceeding 90%, outperforming existing dielectric nanocomposites fabricated via conventional methods. The force-driven growth of nanostructured inorganic fillers within polymer matrices proposes a facile and universal approach for developing high-performance polymer nanocomposites with multi-functionality.</p>","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500178"},"PeriodicalIF":10.7,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Small MethodsPub Date : 2025-06-04DOI: 10.1002/smtd.202401760
Ping Liu, Masayuki Honda, Ryuji Kawano
{"title":"Determination of the position of DNA Methylation and Base Modifications Using a Biological Nanopore.","authors":"Ping Liu, Masayuki Honda, Ryuji Kawano","doi":"10.1002/smtd.202401760","DOIUrl":"https://doi.org/10.1002/smtd.202401760","url":null,"abstract":"<p><p>A method for detecting DNA methylation and modifications is developed using biological nanopores. By exploiting the interaction between bases and acidic amino acids within the entrance and neck region of the α-hemolysin nanopore, we determined the position and frequency of 5-methylcytosine in oligonucleotides. Furthermore, the detection of demethylation intermediates is optimized by examining various ion species and concentrations in the electrolyte. Efforts are also made to employ commercial nanopore devices for high-throughput detection. This approach offers the potential for direct detection of DNA methylation and modifications using biological nanopores.</p>","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2401760"},"PeriodicalIF":10.7,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Small MethodsPub Date : 2025-06-04DOI: 10.1002/smtd.202500693
Björn Altenburger, Joachim Fritzsche, Christoph Langhammer
{"title":"Femtoliter Batch Reactors for Nanofluidic Scattering Spectroscopy Analysis of Catalytic Reactions on Single Nanoparticles.","authors":"Björn Altenburger, Joachim Fritzsche, Christoph Langhammer","doi":"10.1002/smtd.202500693","DOIUrl":"https://doi.org/10.1002/smtd.202500693","url":null,"abstract":"<p><p>Macroscopic batch reactors are a core concept in chemical synthesis and catalysis due to their ability to ensure high conversion rates of the used reactants. At the nanoscale, such reactors hold promise due to their potential to enable chemistry in confinement under well-controlled mass transport conditions, and as enablers for the characterization of catalytic reactions on tiny active surface areas, such as single nanoparticles. However, their practical implementation and the readout of reaction products if used for the study of catalytic reactions is challenging due to their tiny volume and the requirement of being able to transiently open and close such nanoscopic batch reactors. Here, a liquid phase nanofluidic batch reactor with a volume of 4.8 femtoliters is introduced, which conveniently can be opened and closed using a bypassing N<sub>2</sub> gas stream. In combination with nanofluidic scattering spectroscopy (NSS) readout it enables the characterization of a catalytic reaction on a single nanoparticle inside the reactor, as demonstrated on the example of the catalytic reduction of fluorescein by sodium borohydride on a Au catalyst.</p>","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500693"},"PeriodicalIF":10.7,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Small MethodsPub Date : 2025-06-04DOI: 10.1002/smtd.202500729
Taeha Lee, Yeongjun Seo, Dain Kang, Seung Hyeon Oh, Sang Won Lee, Insu Park, Gyudo Lee
{"title":"The 96-Puddle Paper Plate as a Versatile Lab-on-Paper Platform.","authors":"Taeha Lee, Yeongjun Seo, Dain Kang, Seung Hyeon Oh, Sang Won Lee, Insu Park, Gyudo Lee","doi":"10.1002/smtd.202500729","DOIUrl":"https://doi.org/10.1002/smtd.202500729","url":null,"abstract":"<p><p>Microfluidic paper-based analytical devices (µPADs) have facilitated the detection of a wide range of analytes, spanning human health indicators and environmental contaminants. However, their diverse designs necessitate specialized analytical equipment, which complicates standardization, limits productivity, impedes universal analysis, and hinders the acquisition of reliable data. To address these issues, a 96-Puddle Paper Plate (96-PPP) is presented, a versatile lab-on-paper platform that is fully compatible with microplate readers. The 96-PPP offers significant advantages in high-throughput analysis, enabling both qualitative assessments using visual inspection and smartphone cameras, as well as quantitative measurements of absorbance and fluorescence. The versatility of this platform is demonstrated by its ability to detect a wide range of key analytes, including acetone, magnesium ions (Mg<sup>2+</sup>), ascorbic acid, and ketone, which is pertinent to clinical diagnostics, metabolic research, and environmental monitoring. Additionally, 96-PPP enables the study of amyloid deposition (variation within 3.5% across 480 puddles) and proteolysis in cellulose networks that resemble extracellular matrix structures, a capability not achievable with conventional 96-well plates. Overall, 96-PPP replicates the functionality of 96-well plates and concurrently addresses the limitations of conventional µPADs, such as low productivity, versatility, and reliability. Furthermore, it provides unprecedented scalability via a stable, cellulose-based sheet.</p>","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500729"},"PeriodicalIF":10.7,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Small MethodsPub Date : 2025-06-04DOI: 10.1002/smtd.202500122
Mingming Cao, Alexander Brennan, Ciaran M Lee, So-Hyun Park, Gang Bao
{"title":"Deep Learning Based Models for CRISPR/Cas Off-Target Prediction.","authors":"Mingming Cao, Alexander Brennan, Ciaran M Lee, So-Hyun Park, Gang Bao","doi":"10.1002/smtd.202500122","DOIUrl":"https://doi.org/10.1002/smtd.202500122","url":null,"abstract":"<p><p>CRISPR/Cas genome editing technologies enable effective and controlled genetic modifications; however, off-target effects remain a significant concern, particularly in clinical applications. Experimental and in silico methods are developed to predict potential off-target sites (OTS), including deep learning based methods, which can automatically and comprehensively learn sequence features, offer a promising tool for OTS prediction. Here, this work reviews the existing OTS prediction tools with an emphasis on deep learning methods, characterizes datasets used for deep learning training and testing, and evaluates six deep learning models -CRISPR-Net, CRISPR-IP, R-CRISPR, CRISPR-M, CrisprDNT, and Crispr-SGRU -using six public datasets and validates OTS data from the CRISPRoffT database. Performance of these models is assessed using standardized metrics, such as Precision, Recall, F1 score, MCC, AUROC and PRAUC. This work finds that incorporating validated OTS datasets into model training enhanced overall model performance, and improved robustness of prediction, particularly with highly imbalanced datasets. While no model consistently outperforms other models across all scenarios, CRISPR-Net, R-CRISPR, and Crispr-SGRU show strong overall performance. This analysis demonstrates the importance of integrating high-quality validated OTS data with advanced deep learning architectures to improve CRISPR/Cas off-target site predictions, ensuring safer genome editing applications.</p>","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500122"},"PeriodicalIF":10.7,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Small MethodsPub Date : 2025-06-04DOI: 10.1002/smtd.202500374
Hong Chen, Roland Schoch, Jean-Noel Chotard, Yannick M Thiebes, Kerstin Wissel, Rainer Niewa, Matthias Bauer, Oliver Clemens
{"title":"Revealing an Intercalation Nature of High-Capacity Conversion Cathode Materials for Fluoride-Ion Batteries by Operando Studies.","authors":"Hong Chen, Roland Schoch, Jean-Noel Chotard, Yannick M Thiebes, Kerstin Wissel, Rainer Niewa, Matthias Bauer, Oliver Clemens","doi":"10.1002/smtd.202500374","DOIUrl":"https://doi.org/10.1002/smtd.202500374","url":null,"abstract":"<p><p>To improve the performance of high-energy-density electrode materials for all-solid-state fluoride-ion batteries (ASSFIBs), it is important to understand the structure and phase evolution during operation, which is closely correlated to capacity fading. In this study, an operando cell is designed compatible with laboratory X-ray diffraction (XRD) to monitor real-time structural changes of bismuth trifluoride (BiF<sub>3</sub>) cathodes and degradation of the ionic conductor BaSnF<sub>4</sub> under negative potentials at 100 °C. Supported by ex-situ XRD, our results reveal a multi-step defluorination of BiF<sub>3</sub>: from orthorhombic (o-BiF<sub>3</sub>) to cubic (c-BiF<sub>3</sub>), then to distorted orthorhombic (o'-BiF<sub>3</sub>), and finally to metallic bismuth (Bi), indicating partial intercalation-type character. Formation of bismuth oxidefluoride (BiOF) beyond 200 mAh g<sup>-1</sup> is attributed to oxygen impurities introduced via solid-state synthesis. operando X-ray absorption spectroscopy (XAS) confirms a continuous reduction of Bi<sub>3+</sub> to Bi<sub>0</sub> with intermediate phases. Rietveld refinement quantifies the phase fractions and structural transitions, enabling a model for BiF<sub>3</sub> defluorination. Comparison of operando XRD and XAS reveals that BaSnF<sub>4</sub> contributes transport of both fluoride and oxygen impurities, leading to BiOF formation. BaSnF<sub>4</sub> also exhibits a broad stability window, with degradation occurring below -200 mV, rather than the expected -50 mV vs. Sn/SnF<sub>2</sub>.</p>","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500374"},"PeriodicalIF":10.7,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"All-Solid-State S-Scheme Heterojunction TiO<sub>2</sub>/Cu/MoSi<sub>2</sub>N<sub>4</sub> for Efficient Visible-Light Driven Water Splitting.","authors":"Qixing Wang, Shengjia Li, Gang Yuan, Zhengwang Cheng, Zhihui Yang, Hui Lv, Zhuo Peng, Changcun Han, Wei Zou, Fhulufhelo Nemangwele, Jiyan Liu, Xinguo Ma","doi":"10.1002/smtd.202402265","DOIUrl":"https://doi.org/10.1002/smtd.202402265","url":null,"abstract":"<p><p>In the fields of new energy and environmental protection, the development of highly efficient, low-cost, eco-friendly, and stable photoelectrocatalysts has drawn significant interest. Inspired by the high redox potential of S-scheme heterojunctions and the structural advantage of all-solid-state Z-scheme junctions, a novel all-solid-state S-scheme heterojunction TiO<sub>2</sub>/Cu/MoSi<sub>2</sub>N<sub>4</sub> nanorod (NR) array is designed and prepared using hydrothermal and magnetron sputtering methods. Under the synergistic effect of the built-in electric field, high redox potential, and conductive Cu medium, light absorption is extended to the visible-light region, and the separation and transfer efficiencies of the photogenerated carriers are significantly improved. As a result, under > 420 nm visible-light irradiation, the photocurrent density is enhanced by 2.91 times to -18.24 mA cm<sup>-2</sup> at -1.39 V versus reversible hydrogen electrode, and the surface photovoltage is also increased by 7.77 times. Furthermore, the photoelectrochemical (PEC) H<sub>2</sub> evolution rate of TiO<sub>2</sub>/Cu/MoSi<sub>2</sub>N<sub>4</sub> is improved to 1.76 µmol cm<sup>-2</sup> h<sup>-1</sup> and exhibits robust stability. The enhancement mechanism of the PEC performance is systematically explored by combining the experimental results with first-principles calculations. The findings indicate that the construction of an all-solid-state S-scheme heterojunction is a promising strategy to improve PEC performance and can be applied to other photoelectrocatalysts.</p>","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2402265"},"PeriodicalIF":10.7,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}