Small Methods最新文献_第5页

Ultralow-Noise Perovskite Photodiodes Enabling High-Fidelity Full-Color Imaging (Small Methods 19/2026) 实现高保真全彩成像的超低噪声钙钛矿光电二极管（Small Methods 19/2026）

IF 8.7 2区材料科学

Small Methods Pub Date : 2026-10-07 DOI: 10.1002/smtd.71000

Chun-Jen Shih, Galing Murokinas, Meng-Chi Li, Shun-Wei Liu

引用次数: 0

Rational Sulfur Vacancy Design Enhances the Electrochemical and Nonlinear Optical Properties of V2CTx/WS2 合理的硫空位设计提高了V2CTx/WS2的电化学和非线性光学性能。

IF 8.7 2区材料科学

Small Methods Pub Date : 2026-10-07 Epub Date: 2026-09-02 DOI: 10.1002/smtd.71016

Fei Hu, Jinhe Wei, Xinyu Quan, Zixuan Jiang, Qiuyun Ouyang

{"title":"Rational Sulfur Vacancy Design Enhances the Electrochemical and Nonlinear Optical Properties of V2CTx/WS2","authors":"Fei Hu, Jinhe Wei, Xinyu Quan, Zixuan Jiang, Qiuyun Ouyang","doi":"10.1002/smtd.71016","DOIUrl":"10.1002/smtd.71016","url":null,"abstract":"<div>\u0000 \u0000 The concurrent optimization of electrochemical energy storage and third-order nonlinear optical (NLO) performance within a single material system remains a fundamental challenge. Here, V2CTx/WS2 composites were fabricated via a solvothermal method on carbon cloth substrates, and (V2CTx/WS2)x/PMMA were prepared for optical measurements. Interfacial interactions between V2CTx surface functional groups and WS2 promote the formation of sulfur vacancies and interfacial charge redistribution during compositing. The enlarged interlayer spacing, three-dimensional WS2 nanoflower architecture, and defect-rich structure collectively increase accessible active sites and facilitate charge transport. The V2CTx/WS2 electrode delivers a specific capacity of 2251.95 F·g−1 at 1 A·g−1, retaining 94.80% of its initial capacity after 10 000 cycles. The assembled supercapacitor achieves an energy density of 47.93 Wh·kg−1 at a power density of 983.45 W·kg−1. For NLO performance, the (V2CTx/WS2)8/PMMA sample exhibits a nonlinear absorption coefficient β of 232 cm·GW−1, a nonlinear refraction coefficient γ of −9.29 × 10−4 cm2·GW−1, and an optical limiting threshold of 1.09 J·cm−2. The results demonstrate that V2CTx/WS2 exhibits excellent electrochemical and NLO properties through composite structure design and defect engineering.\u0000 </div>","PeriodicalId":229,"journal":{"name":"Small Methods","volume":"10 19","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148872589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Label-Free Profiling of Immunoglobulin Isotypes Using Solid-State Nanopores 利用固态纳米孔对免疫球蛋白同型进行无标记分析。

IF 8.7 2区材料科学

Small Methods Pub Date : 2026-10-07 Epub Date: 2026-06-02 DOI: 10.1002/smtd.70758

Iris Baffour Ansah, Kevin J. Freedman

{"title":"Label-Free Profiling of Immunoglobulin Isotypes Using Solid-State Nanopores","authors":"Iris Baffour Ansah, Kevin J. Freedman","doi":"10.1002/smtd.70758","DOIUrl":"10.1002/smtd.70758","url":null,"abstract":"<div>\u0000 \u0000 Accurate profiling of antibody isotypes is essential to monitor immune responses, diagnose disease, and guide therapy, yet existing immunoassays remain limited in capturing their conformational dynamics with molecular precision. Nanopore sensing offers a label-free route to such resolution, but most implementations rely on fragmentation, probes, or functionalization, leaving intact isotypes inaccessible. Here, a nanopore-based assay that directly discriminates full-length IgG, monomeric IgA, and IgM under non-denaturing conditions, resolving intrinsic molecular signatures in real time is presented. In a lithium chloride electrolyte, glass nanopipettes operate in an electroosmotic-flow–dominated regime where flow-driven transport and nanoscale confinement produce distinct ionic fingerprints for each isotype. Affinity-based signal modulation with protein G, which selectively binds the IgG Fc domain, further introduces a tunable contrast mechanism that sharpens IgG-IgA separability while preserving native structure and binding functionality. Quantitative analysis demonstrates robust separation (p < 0.0001) in binary and ternary mixtures with low misclassification. Evaluation in diluted human serum with a physiologically representative IgG:IgA:IgM ratio (7:2:1) shows that isotype-specific fingerprints remain distinguishable in a serum-derived background. The EOF-governed assay establishes a scalable framework for direct, multiplexed immunoprofiling and extends label-free nanopore analysis beyond antibodies toward broader proteomic targets.\u0000 </div>","PeriodicalId":229,"journal":{"name":"Small Methods","volume":"10 19","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148142156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transfer-Stacked and Pre-Tunable Bilayer Photoresist for High-Yield Dry Lift-off and Multiscale Patterning 用于高产量干式脱模和多尺度图案的转移堆叠和预可调双层光刻胶。

IF 8.7 2区材料科学

Small Methods Pub Date : 2026-10-07 Epub Date: 2026-09-01 DOI: 10.1002/smtd.71008

Quan Wang, Xiaoxue Bi, Shaopeng Yu, Huikang Liang, Yanting Lv, Lei Chen, Bo Feng, Huigao Duan

{"title":"Transfer-Stacked and Pre-Tunable Bilayer Photoresist for High-Yield Dry Lift-off and Multiscale Patterning","authors":"Quan Wang, Xiaoxue Bi, Shaopeng Yu, Huikang Liang, Yanting Lv, Lei Chen, Bo Feng, Huigao Duan","doi":"10.1002/smtd.71008","DOIUrl":"10.1002/smtd.71008","url":null,"abstract":"<div>\u0000 \u0000 Photolithography, a cornerstone of semiconductor manufacturing, faces persistent challenges in lift-off processes, particularly regarding solvent usage, sidewall adhesion, and limited pattern fidelity. While dry lift-off and bilayer resist schemes provide promising alternatives, conventional approaches often rely on multiple materials and exhibit uncontrollable undercut profiles. Here, we report a universal bilayer photoresist strategy that uses a single material and enables solvent-free, high-yield dry lift-off. By integrating surface modification and UV pre-exposure (222 nm), our method allows precise control of the undercut structure and maintains full compatibility with subsequent processes such as sputtering and dry lift-off. Experiments demonstrate robust multiscale patterning capability, achieving from wafer-scale down to 17 nm features, with a 100% yield, high density (line width: spacing = 5:4), and clean sidewalls without residue. This strategy also enables high-resolution quantum dot patterning and is compatible with the fabrication of pixelated perovskite micro-LEDs, highlighting its potential for optoelectronic applications including displays, optical encryption, and anti‑counterfeiting.\u0000 </div>","PeriodicalId":229,"journal":{"name":"Small Methods","volume":"10 19","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148863140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Filtration-Based SERS With In Situ Gold Growth in Bacterial Microenvironment for Rapid Detection of Low-Level Bacteria 细菌微环境中原位金生长的基于过滤的SERS快速检测低水平细菌。

IF 8.7 2区材料科学

Small Methods Pub Date : 2026-10-07 Epub Date: 2026-08-16 DOI: 10.1002/smtd.70972

Eun-Bi Jang, Ji Young Lee, Hoeil Chung, Min-Young Lee

{"title":"Filtration-Based SERS With In Situ Gold Growth in Bacterial Microenvironment for Rapid Detection of Low-Level Bacteria","authors":"Eun-Bi Jang, Ji Young Lee, Hoeil Chung, Min-Young Lee","doi":"10.1002/smtd.70972","DOIUrl":"10.1002/smtd.70972","url":null,"abstract":"Rapid bacterial detection and timely antimicrobial susceptibility assessment are essential for effective infection management, yet remain challenging under clinically relevant low-concentration conditions. Here, we present a filtration-based surface-enhanced Raman scattering (SERS) platform based on a microenvironment engineering strategy, in which plasmonic nanostructures are dynamically formed within the bacterial microenvironment. Using a gold nanostructured glass microfiber membrane, the platform enables large-volume sample processing through size-selective bacterial capture, followed by in situ gold growth that generates bacteria-centered plasmonic hotspots directly around bacterial cells. This architecture fundamentally overcomes the intrinsic hotspot–bacteria mismatch in conventional substrate-based SERS systems, enabling highly sensitive and selective detection while effectively suppressing nonspecific interference in complex biological matrices. To further address sampling limitations at low concentrations, a minimal pre-culture strategy was introduced to enhance bacterial surface occupancy, improving the detection limit from 103 to 101 CFU/mL. In addition, SERS signal intensity directly reflects bacterial viability, allowing rapid differentiation of antimicrobial responses and enabling susceptibility assessment within 2 h. By integrating physical enrichment with microenvironment-driven signal amplification, this platform simultaneously achieves low-level detection and rapid antimicrobial susceptibility testing within a single system. These results highlight its potential as a next-generation integrated diagnostic platform for clinical sample analysis.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":"10 19","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/smtd.70972","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148757117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wearable Sensing Devices for Continuous Animal Health Monitoring 用于持续动物健康监测的可穿戴传感设备。

IF 8.7 2区材料科学

Small Methods Pub Date : 2026-10-07 Epub Date: 2026-08-18 DOI: 10.1002/smtd.70982

Keer Chen, Youmin Zheng, Yifan Gu, Guozhen Liu

{"title":"Wearable Sensing Devices for Continuous Animal Health Monitoring","authors":"Keer Chen, Youmin Zheng, Yifan Gu, Guozhen Liu","doi":"10.1002/smtd.70982","DOIUrl":"10.1002/smtd.70982","url":null,"abstract":"<div>\u0000 \u0000 Continuous, noninvasive animal health monitoring systems are increasingly required in livestock farming, companion animal care, and wildlife conservation. The translation of wearable sensing technologies across these sectors is complicated. Their practical performance depends not only on sensing accuracy but also on the variability of anatomical features, uncontrolled movement, environmental exposure, wireless connectivity, power supply, and animal welfare considerations. This review presents a system-level, design-oriented analysis of animal health monitoring sensing technologies. It classifies sensing modalities into physical and physiological, biochemical and molecular, environmental and spatial, and multimodal sensing, followed by examining their integration into on-animal and ingestible devices. Communication, edge and cloud computing, power management, and representative applications across livestock, companion animals, and wildlife are also discussed. The review further identifies key barriers to translation, including the need for signal stability, interoperability between systems, energy supply constraints, maintenance burden, and ethical and welfare concerns related to long-term monitoring. Future systems should prioritize species- and site-specific integration, long-term field validation, interoperable data architectures, welfare-conscious design, and clinically or managerially actionable outputs. This framework offers practical guidance for translating continuous sensing into scalable decisions for animal health and welfare.\u0000 </div>","PeriodicalId":229,"journal":{"name":"Small Methods","volume":"10 19","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148786389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vat Polymerization 3D Printing of Hydrogels With Tunable Porosity 孔隙度可调水凝胶的还原聚合3D打印。

IF 8.7 2区材料科学

Small Methods Pub Date : 2026-10-07 Epub Date: 2026-08-28 DOI: 10.1002/smtd.70988

Liwei Liu, Liwen Zhang, Xumin Huang, Yuan Xu, Jason R. Stokes, Xinyan Gu, Naufal Kabir Ahamed Nasar, Xiaoming Sun, Jiali Zhai, Thomas P. Davis, Ruirui Qiao

{"title":"Vat Polymerization 3D Printing of Hydrogels With Tunable Porosity","authors":"Liwei Liu, Liwen Zhang, Xumin Huang, Yuan Xu, Jason R. Stokes, Xinyan Gu, Naufal Kabir Ahamed Nasar, Xiaoming Sun, Jiali Zhai, Thomas P. Davis, Ruirui Qiao","doi":"10.1002/smtd.70988","DOIUrl":"10.1002/smtd.70988","url":null,"abstract":"Three-dimensional, interconnected hydrogel networks are central to tissue engineering and disease modeling, where tailored pore architecture and mechanical robustness are essential for supporting cellular functions. However, the limited ability to engineer microstructural features in vat polymerization 3D-printed natural hydrogels often compromises scaffold performance, as oversized pores reduce cell attachment and cell–cell interactions while smooth pore walls lack essential topographical cues. Here, we report an emulsion-based ink for vat polymerization 3D printing that enables the fabrication of hydrogels with finely tunable and highly interconnected porous architectures. An oil-in-water resin formulated using gelatin methacrylate (GelMA) contains stable solvent nanodroplets that act as sacrificial templates during photopolymerization. Removal of the dispersed phase yields additive-free porous hydrogels with pore sizes ranging from 0.66 to 46.15 µm and a 2.5-fold enhancement in compressive toughness. This strategy is compatible with digital light processing (DLP) and broadly applicable to multiple photocurable biopolymers, including alginate methacrylate (ALMA) and hyaluronic acid methacrylate (HAMA). The resulting porous scaffolds promote enhanced cell attachment, proliferation, and cell–cell interactions, highlighting the potential of this vat polymerization-compatible platform for advanced biofabrication.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":"10 19","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/smtd.70988","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148849517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two-Dimensional CuTCNQ Built From Supercritical CO₂-Induced Conformational Transform for Charge Transfer and Electron Modulation. 利用超临界co2诱导的电荷转移和电子调制构象变换建立二维cucnq。

IF 8.7 2区材料科学

Small Methods Pub Date : 2026-10-07 DOI: 10.1002/smtd.71101

Weiqian Kong, Pengfei Yan, Song Xu, Zongwei Chen, Xiaoli Zheng, Wei Liu, Wenzhuo Wu, Qingyong Tian, Qun Xu

{"title":"Two-Dimensional CuTCNQ Built From Supercritical CO2-Induced Conformational Transform for Charge Transfer and Electron Modulation.","authors":"Weiqian Kong, Pengfei Yan, Song Xu, Zongwei Chen, Xiaoli Zheng, Wei Liu, Wenzhuo Wu, Qingyong Tian, Qun Xu","doi":"10.1002/smtd.71101","DOIUrl":"https://doi.org/10.1002/smtd.71101","url":null,"abstract":"The two-dimensional (2D) metal-organic frameworks (MOFs) features intrinsic structural peculiarities and high structural design ability, which is considered as a unique platform for prominent electrical and optical applications. A deeper understanding of the electrical and optical properties that are determined by the function of 2D MOFs is crucial for advancing optoelectronic applications. Herein, we propose a novel strategy to enhance light utilization, charge transfer ability and electron dynamics of 2D MOFs by modulating the coordinative environment. The bulk copper tetracyanoquinodimethane (CuTCNQ) is converted into 2D nanosheets (2D CuTCNQ) via the assistance of supercritical carbon dioxide (SC CO2). The solvent effects of SC CO2 significantly tune the thermodynamical kinetic of bulk CuTCNQ, leading to partial detachment of ligands and the formation of 2D CuTCNQ with unique electron configuration. Simultaneously, 2D CuTCNQ exhibits broadened visible light absorption and accelerated electronic kinetics in comparison with bulk CuTCNQ. Further, the optimized 2D CuTCNQ was employed as a photoanode and it exhibits significantly enhanced photoelectrochemical (PEC) performance, highlighting the potential application in optoelectronic technology. Therefore, such coordinative environment modulation strategy via SC CO2 opens new avenues to optimize photoelectric properties, and as well as to provide rational design on multifunctional 2D MOFs.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e71101"},"PeriodicalIF":8.7,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149126205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights Into Mg²⁺-Driven Lattice Stabilization and Na⁺ Kinetics Regulation in O3-Type NaMn_0.5Fe_0.5O₂ Cathodes for Sodium-Ion Batteries. 钠离子电池用o3型NaMn0.5Fe0.5O2阴极中Mg2+驱动晶格稳定和Na+动力学调控的研究

IF 8.7 2区材料科学

Small Methods Pub Date : 2026-10-07 DOI: 10.1002/smtd.71086

Faseeh Ul Zaman, Saleem Sidra, Van Hien Hoa, Sang Won Lee, Ghulam Ali, Do Hwan Kim

{"title":"Insights Into Mg2+-Driven Lattice Stabilization and Na+ Kinetics Regulation in O3-Type NaMn0.5Fe0.5O2 Cathodes for Sodium-Ion Batteries.","authors":"Faseeh Ul Zaman, Saleem Sidra, Van Hien Hoa, Sang Won Lee, Ghulam Ali, Do Hwan Kim","doi":"10.1002/smtd.71086","DOIUrl":"https://doi.org/10.1002/smtd.71086","url":null,"abstract":"Sodium-ion batteries (SIBs) are emerging as cost-effective alternatives to lithium-ion systems owing to the natural abundance of sodium. Among layered cathodes, O3-type NaFe0.5Mn0.5O2 exhibits high theoretical capacity and environmental compatibility but suffers from structural degradation driven by O3→P3 transitions, volume variation, and transition-metal migration during cycling. Herein, Mg2+ is incorporated to regulate the lattice stability and electrochemical performance of NaFe0.5Mn0.5O2 via grain boundaries/interface difustion at solid state. The sample Mg4-NFM was synthesized via a solid-state route and systematically characterized. The x-ray diffraction combined with Williamson-Hall analysis reveals Mg-induced lattice modulation, micro-strain regulation, and crystallite-size variation, while SEM, FTIR, Raman spectroscopy, and BET measurements provide complementary insights into morphology, bonding, vibrational characteristics, and surface properties. Electrochemical evaluations demonstrate that the 0.48 At% Mg atoms in the hybrid sample deliver the optimal performance, achieving an initial discharge capacity of 83.3 mAh g- 1 with excellent capacity retention over 100 cycles at 0.1C and Coulombic efficiency exceeding 98.5%. Density functional theory calculations reveal that Mg incorporation enhances thermodynamic stability and increases the density of states near the Fermi level, indicating improved electronic conductivity. Furthermore, nudged elastic band calculations identify an ultralow Na+ diffusion barrier of 0.113 eV, confirming favorable ion-transport kinetics.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e71086"},"PeriodicalIF":8.7,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149126156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Power of Pristine Interfaces: Unmodified Glass Nanopores for Nucleic Acid Analysis 原始界面的力量：未经修饰的核酸分析玻璃纳米孔。

IF 8.7 2区材料科学

Small Methods Pub Date : 2026-10-07 Epub Date: 2026-09-08 DOI: 10.1002/smtd.71020

Zhaowei Guan, Dezhi Feng, Jinbo Zhu

{"title":"The Power of Pristine Interfaces: Unmodified Glass Nanopores for Nucleic Acid Analysis","authors":"Zhaowei Guan, Dezhi Feng, Jinbo Zhu","doi":"10.1002/smtd.71020","DOIUrl":"10.1002/smtd.71020","url":null,"abstract":"<div>\u0000 \u0000 Detection of nucleic acids at the single-molecule level is a cornerstone of next-generation diagnostics. Unmodified glass nanopore-based sensing stands out among single-molecule detection techniques for its simplicity and versatility. This review presents the key advantages of unmodified glass nanopore, including its chemically homogeneous interface, tunable conical geometry, and inherent ion current rectification, which together enable high-fidelity resistive pulse sensing of nucleic acids. We propose a classification framework that differentiates two categories of methods. Amplification-free strategies retain molecular fidelity and conformational dynamics, while amplification-coupled approaches overcome limitations of low target concentrations to achieve ultrasensitive detection. Furthermore, we highlight recent breakthroughs, including standardized encapsulation for long-term stability, automated declogging systems, and the combination of DNA nanotechnology approaches to enhance specificity. By integrating these advancements, this review provides a comprehensive framework for understanding unmodified glass nanopore technology and outlines a strategic roadmap for developing integrated, intelligent, and portable single-molecule analytical tools for precision medicine.\u0000 </div>","PeriodicalId":229,"journal":{"name":"Small Methods","volume":"10 19","pages":""},"PeriodicalIF":8.7,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148910136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0