Small Methods最新文献_第9页

Identification of Multi-Landscape and Cell Interactions in the Tumor Microenvironment Through High-Coverage Single-Cell Sequencing. 通过高覆盖单细胞测序鉴定肿瘤微环境中的多景观和细胞相互作用。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-05-05 DOI: 10.1002/smtd.202500241

Wenlong Zhong, Ligang Wang, Chengjunyu Zhang, Tonglei Guo, Lihua Zhao, Daqin Wu, Fei Xie, Xiao Wang, Xiuxin Li, Fangxiao Wang, Minghui Li, Weiyue Gu, Tianxin Lin, Xu Chen

{"title":"Identification of Multi-Landscape and Cell Interactions in the Tumor Microenvironment Through High-Coverage Single-Cell Sequencing.","authors":"Wenlong Zhong, Ligang Wang, Chengjunyu Zhang, Tonglei Guo, Lihua Zhao, Daqin Wu, Fei Xie, Xiao Wang, Xiuxin Li, Fangxiao Wang, Minghui Li, Weiyue Gu, Tianxin Lin, Xu Chen","doi":"10.1002/smtd.202500241","DOIUrl":"https://doi.org/10.1002/smtd.202500241","url":null,"abstract":"Single-cell RNA sequencing (scRNA-seq) is a widely used method for classifying cell types and states and revealing disease mechanisms. However, most contemporary scRNA-seq platforms fail to explore the multilandscape of RNA. Here, a microfluidic chip is designed that combines oligo-dT primers and Random Bridging Co-labeling (RBCL) RNA sequencing to develop an innovative Chigene scRNA-seq technology that can identify gene expression, mutations, and RNA splicing landscapes at the single-cell level. The Chigene scRNA-seq platform demonstrated exceptional performance, with minimal doublet rates of 0.94% (Chigene V1) and 1.93% (Chigene V2). Both versions exhibit high sensitivity, with Chigene V2 achieving nearly 100% RNA coverage and detecting over 1800 genes per cell on average. Targeted capture of single-cell gene mutations enhances mutation detection sensitivity. Moreover, this Chigene V2 platform is validated in clinical samples for its ability to detect mutations, gene fusions, and alternative splicing. The reliability of the platform is further corroborated via known functional gene mutation (CDKN1A) and fusion (FGFR3-TACC). To validate this method's potential for discovering novel gene mutations in clinical samples, the investigation revealed an intriguing cell subpopulation carrying an ARHGAP5 mutation in urothelial carcinoma. These cells exhibited high-frequency mRNA splicing and exhibited specific crosstalk with T cells, distinguishing them from the subpopulation with the ARHGAP5 wild-type phenotype. Overall, this method provides a robust scRNA-seq platform suitable for comprehensive analyses of clinical specimens at different genetic information levels, thereby offering significant potential in the discovery of novel genes and interactions at the single-cell level.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500241"},"PeriodicalIF":10.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systematically Navigating Through the Parameter Space During the Lateral Manipulation of PTCDA on the Ag(111) Surface. Ag（111）表面PTCDA横向操纵过程中参数空间的系统导航。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-05-04 DOI: 10.1002/smtd.202500177

Tim Dierker, Paul Laubrock, Philipp Rahe

{"title":"Systematically Navigating Through the Parameter Space During the Lateral Manipulation of PTCDA on the Ag(111) Surface.","authors":"Tim Dierker, Paul Laubrock, Philipp Rahe","doi":"10.1002/smtd.202500177","DOIUrl":"https://doi.org/10.1002/smtd.202500177","url":null,"abstract":"The lateral manipulation of single perylene-3,4,9,10-tetracarboxylic dianhydride (PTCDA) molecules serves as a key model process for both building surface-supported nanostructures and enabling quantum sensing with single molecules attached to scanning probe microscopy tips. This work introduces an instructive procedure that guarantees the controlled lateral movement of single PTCDA molecules, in particular the isolation from molecular island edges. The lateral manipulation relies on establishing a specific bond between one of the molecular carboxylic oxygen atoms and the metallic tip of a combined scanning tunneling (STM) and atomic force microscope (AFM) before displacing the molecule by laterally moving the tip. From analyzing both the tip-position data during this movement and the STM imaging contrast after the manipulation, a categorization scheme containing four resulting tip-molecule-surface configurations is proposed. Together with transitions observed between some of these configurations, the complex tip-molecule-surface system parameter space during the manipulation procedure can be compressed into an instructive flowchart. Following through this flowchart guarantees the lateral isolation of a single PTCDA molecule in a systematic manner and without requirement for previous knowledge. Broad applicability is verified by also manipulating molecules from Ag(111) surface step-edges and from molecular island edges on the Au(111) surface.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500177"},"PeriodicalIF":10.7,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tailoring in-Plane Permittivity Gradients by Shadow Mask Molecular Beam Epitaxy. 利用阴影掩膜分子束外延裁剪面内介电常数梯度。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-05-03 DOI: 10.1002/smtd.202500361

Shagorika Mukherjee, Sai Rahul Sitaram, Mingyu Yu, Xi Wang, Stephanie Law

{"title":"Tailoring in-Plane Permittivity Gradients by Shadow Mask Molecular Beam Epitaxy.","authors":"Shagorika Mukherjee, Sai Rahul Sitaram, Mingyu Yu, Xi Wang, Stephanie Law","doi":"10.1002/smtd.202500361","DOIUrl":"https://doi.org/10.1002/smtd.202500361","url":null,"abstract":"Infrared (IR) gradient permittivity materials are the potential building blocks of miniature IR-devices such as an on-chip spectrometer. The manufacture of materials with permittivities that vary in the horizontal plane is demonstrated using shadow mask molecular beam epitaxy in Si:InAs films. However, to be useful, the permittivity gradient needs to be of high crystalline quality and its properties need to be tunable. In this paper, it is shown that it can control the permittivity gradient length and steepness by varying the shadow mask thickness. Samples grown with similar growth parameters and with 200 and 500 µm mask thicknesses show permittivity gradient widths of 18 and 39 µm on the flat mesa on one side and 11 and 23 µm on the film slope on the other side, respectively. The gradient steepnesses are 23.3 and 11.3 cm-1/µm on the flat mesa and 21.8 and 9.1 cm-1/µm on the film slope, for samples made with the 200 and 500 µm masks, respectively. This work clearly shows the ability to control the in-plane permittivity gradient in Si:InAs films, setting the stage for the creation of miniature IR devices.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500361"},"PeriodicalIF":10.7,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of Interfacial Reactions on Perovskite Optoelectronic Devices. 界面反应对钙钛矿光电器件的影响。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-05-03 DOI: 10.1002/smtd.202500438

Zhongcheng Yuan, Sai Bai, Feng Gao, Henry J Snaith

引用次数: 0

Unveiling the Mysterious Structure Growth of 2D and 3D All-Inorganic Perovskite Nanocrystals in Solution Phase Dynamically by Using Small-Wide Angle X-Ray Scattering Spectroscopy. 用小广角x射线散射光谱动态揭示二维和三维全无机钙钛矿纳米晶体在溶液中的神秘结构生长。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-05-03 DOI: 10.1002/smtd.202500065

Kuan-Chang Wu, Je-Wei Chang, Yi-Chia Chen, Yu-Dian Chen, Jou-Chun Lin, Ying-Huang Lai, Chun-Jen Su, Di-Yan Wang

{"title":"Unveiling the Mysterious Structure Growth of 2D and 3D All-Inorganic Perovskite Nanocrystals in Solution Phase Dynamically by Using Small-Wide Angle X-Ray Scattering Spectroscopy.","authors":"Kuan-Chang Wu, Je-Wei Chang, Yi-Chia Chen, Yu-Dian Chen, Jou-Chun Lin, Ying-Huang Lai, Chun-Jen Su, Di-Yan Wang","doi":"10.1002/smtd.202500065","DOIUrl":"https://doi.org/10.1002/smtd.202500065","url":null,"abstract":"Real-time analysis of the structural formation of 2D and 3D perovskites in solution is challenging due to the sensitivity of perovskite intermediates to environmental conditions and their rapid growth. Conventional techniques often require stringent sample preparation, limiting the ability to study dynamic behaviors in solution. In this study, small- and wide-angle X-ray scattering (SWAXS) is employed to analyze the morphology and dynamics of 2D and 3D perovskite nanostructures in their native colloidal state. Unlike previous studies that attribute CsPbI3 degradation to delta-phase formation, SWAXS revealed preexisting 2D Cs7Pb6I19 nanosheets in pristine CsPbI3 colloidal solutions. In situ SWAXS tracked the dynamic transformation of these structures during recrystallization in diluted solutions. Adding bis(trimethylsilyl)sulfide (TMS) disassembled the 2D nanosheets, while subsequent recrystallization in a poor solvent formed highly crystalline Cs7Pb6I19 nanosheets. The recrystallization dynamics aligned with crystal growth theory, with TMS concentration playing a critical role. Higher TMS concentrations slowed recrystallization, promoting stable lattice formation and enhanced crystallinity, resulting in bright yellow emission. Conversely, lower concentrations accelerated recrystallization, causing structural damage and limiting high-crystallinity growth. This study highlights the importance of controlling recrystallization rates through TMS concentration to optimize the crystallinity and optoelectronic properties of perovskites, offering insights into improving their performance.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500065"},"PeriodicalIF":10.7,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Controlling Quantum-Confined Stark Effect in Coupled II-VI Quantum Dots by Interface Engineering. 界面工程控制耦合II-VI量子点的量子受限Stark效应。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-05-03 DOI: 10.1002/smtd.202402262

Pingping Han, Tingli Du, Si Zhou, Jijun Zhao

引用次数: 0

Powder Methodology-An Effective Way to Suppress Sn²⁺ Oxidation in Narrow Bandgap Pb-Sn Perovskite. 粉末法——抑制窄带隙铅锡钙钛矿中Sn2+氧化的有效方法。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-05-02 DOI: 10.1002/smtd.202500522

Mahdi Gassara, Haixuan Yu, Ashish Kulkarni, Mingkui Wang, Samrana Kazim, Shahzada Ahmad

引用次数: 0

Biomimetic Membrane-Like Co/CoSe₂@CNF-CNT (L) with Selective Permeability for Enhanced Performance of Lithium-Sulfur Battery. 具有选择性磁导率的仿生膜状Co/CoSe2@CNF-CNT (L)提高锂硫电池性能

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-05-02 DOI: 10.1002/smtd.202500312

Siqi Wang, Kai Sun, Han Guo, Yiding Li, Yujun Fu, Xiaorong Fang, Ruoan Li, Chengtian Lv, Yinxin Yao, Zhouqing He, Dequan Liu, Deyan He

{"title":"Biomimetic Membrane-Like Co/CoSe2@CNF-CNT (L) with Selective Permeability for Enhanced Performance of Lithium-Sulfur Battery.","authors":"Siqi Wang, Kai Sun, Han Guo, Yiding Li, Yujun Fu, Xiaorong Fang, Ruoan Li, Chengtian Lv, Yinxin Yao, Zhouqing He, Dequan Liu, Deyan He","doi":"10.1002/smtd.202500312","DOIUrl":"https://doi.org/10.1002/smtd.202500312","url":null,"abstract":"Inspired by the multifunctionality and precise regulation of biological membranes, this work designs a biomimetic membrane-like structure for lithium-sulfur (Li-S) batteries. By controlling the selenization time, the composition of the metal catalyst and the material's microstructure is regulated, resulting in a self-supporting 3D porous conductive network of Co/CoSe2-modified carbon nanofiber (CNF) with in situ grown carbon nanotubes (CNT) (Co/CoSe2@CNF-CNT (L)). Drawing inspiration from the selective permeability of biological membranes, the Co/CoSe2@CNF-CNT (L) system achieves dual-functionality. On one hand, the Co/CoSe2 catalyst, possessing Lewis acid properties, provides a strong binding affinity for lithium polysulfides (LiPSs), effectively inhibiting their migration. Additionally, CNF and in situ grown CNT form a microporous structure, which effectively inhibits the shuttle effect. This behavior is analogous to specific proteins in biological membranes that selectively recognize and bind certain substances. On the other hand, the CNF-CNT 3D conductive network has numerous ion channels that ensure efficient lithium-ion (Li+) transport, mirroring the role of ion channels or carrier proteins in biological membranes. Consequently, the cell with the Co/CoSe2@CNF-CNT (L) exhibits a reversible capacity of 1425.21 mAh g-1 at 1 A g-1 and retains 875.33 mAh g-1 after 500 cycles. Furthermore, the cells exhibit excellent stability under high currents and prolonged cycling.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500312"},"PeriodicalIF":10.7,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Achieving Full Redispersion of Dried MXene Monoliths via Trace Metal Cation Intercalation. 通过微量金属阳离子嵌入实现干燥MXene单体的完全再分散。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-05-02 DOI: 10.1002/smtd.202500383

Juyun Lee, Sung Ho Cho, Jeong Min Jang, Seung Hee Woo, Yun Chan Kang, Seon Joon Kim

{"title":"Achieving Full Redispersion of Dried MXene Monoliths via Trace Metal Cation Intercalation.","authors":"Juyun Lee, Sung Ho Cho, Jeong Min Jang, Seung Hee Woo, Yun Chan Kang, Seon Joon Kim","doi":"10.1002/smtd.202500383","DOIUrl":"https://doi.org/10.1002/smtd.202500383","url":null,"abstract":"MXenes, a class of 2D transition metal carbides and nitrides, exhibit exceptional electrical conductivity and solution dispersibility, making them promising materials for various applications. However, their long-term stability remains a critical challenge due to oxidation in aqueous dispersions. While the transformation of these dispersions into water-redispersible dry monoliths is highly desirable, achieving this has proven difficult. This study introduces a facile approach to enhance the redispersion yield of dried MXene monoliths by incorporating trace amounts of metal cations (Li+, Mg2+, and Al3+) into aqueous dispersions prior to lyophilization. These cations intercalate between MXene sheets, acting as atomic pillars that inhibit face-to-face restacking and facilitate water infiltration during redispersion. Systematic investigations reveal that optimal cation concentrations significantly improve redispersion efficiency without inducing flocculation, achieving yields of up to 100% for Li+-modified MXenes. Characterization of redispersed MXene nanosheets confirms preserved morphology and structural integrity. Furthermore, compared to the pristine MXene counterparts, MXene films made from cation-aided redispersions show higher electrical conductivity and electromagnetic interference shielding performances. This simple yet effective strategy addresses key challenges in MXene storage and processing, enabling reliable solution-based fabrication for energy storage, sensing, and electronic applications.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500383"},"PeriodicalIF":10.7,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low Temperature Complexation Approach for Immobilization of Single Copper Atom Catalyst in Stacked Polytriazine for Click Cycloaddition Reaction. 低温络合法固定化单铜原子催化剂在叠聚三嗪上的点击环加成反应。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-05-02 DOI: 10.1002/smtd.202500655

Pratibha Kiran Giri, Anuj Rawat, Mukaddar Sk, Bishnupriya Swain, Ranjit Thapa, Paritosh Mohanty

{"title":"Low Temperature Complexation Approach for Immobilization of Single Copper Atom Catalyst in Stacked Polytriazine for Click Cycloaddition Reaction.","authors":"Pratibha Kiran Giri, Anuj Rawat, Mukaddar Sk, Bishnupriya Swain, Ranjit Thapa, Paritosh Mohanty","doi":"10.1002/smtd.202500655","DOIUrl":"https://doi.org/10.1002/smtd.202500655","url":null,"abstract":"A significant research gap in the field of synthesis of single atom catalysts (SACs) is addressed by developing a low-temperature complexation approach to stabilize the single metal atoms on stacked polytiazine matrix (g-C3N4) with a good metal loading. Unlike conventional high-energy (400-700 °C) and time-intensive (120-300 min) methods typically used for embedding SACs in g-C3N4 matrices, the present synthesis utilizes a facile, microwave-assisted method that operates at a low temperature of 140 °C and completes within 30 min. Comprehensive analysis reveal that complexation of the Cu2+/Cu+ ions with nitrogen in the polytriazine structure facilitates layer stacking. Specifically, Cu⁺ ions promote sheet formation in co-ordination with two nearby N atoms, while Cu2+ ions stabilize the stacked layers of the polytriazine framework through co-ordination with four N atoms. The resulting SAC exhibits a Cu metal loading up to 3.5 wt.%, with a specific surface area (SABET) of 330 m2 g-1 and pore size distribution centered at 1.9 and 5 nm. The SAC demonstrates excellent catalytic performance for click cycloaddition reactions under base-free conditions, with a high turnover frequency (TOF) of 120 h-1, a broad substrate scope, and reusability across seven cycles without detectable Cu leaching, making it a promising SAC for triazole synthesis.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500655"},"PeriodicalIF":10.7,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0