Plant, Cell & Environment最新文献

筛选
英文 中文
The Mysterious Mechanism of Aquaporin-Mediated CO₂ Transport. 水通道蛋白介导的CO₂运输的神秘机制。
IF 6.3 1区 生物学
Plant, Cell & Environment Pub Date : 2025-09-14 DOI: 10.1111/pce.70187
Hui Wang, Huayan Zhao
{"title":"The Mysterious Mechanism of Aquaporin-Mediated CO₂ Transport.","authors":"Hui Wang, Huayan Zhao","doi":"10.1111/pce.70187","DOIUrl":"https://doi.org/10.1111/pce.70187","url":null,"abstract":"","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145062944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cold-Induced Promoter Methylation Attenuates ScMYB7-Mediated Repression of the CBF Pathway: A Proposed Mechanism for Sugarcane Cold Adaptation. 冷诱导启动子甲基化减弱scmyb7介导的CBF通路抑制:甘蔗冷适应的一种可能机制
IF 6.3 1区 生物学
Plant, Cell & Environment Pub Date : 2025-09-14 DOI: 10.1111/pce.70180
Weiwei Liu, Qiuyue Ou, Meichang Feng, Yue Li, Linghan Huang, Xuan Peng, Xiaoqiang Zhong, Guoqiang Huang, Hengbo Wang, Jinlong Guo
{"title":"Cold-Induced Promoter Methylation Attenuates ScMYB7-Mediated Repression of the CBF Pathway: A Proposed Mechanism for Sugarcane Cold Adaptation.","authors":"Weiwei Liu, Qiuyue Ou, Meichang Feng, Yue Li, Linghan Huang, Xuan Peng, Xiaoqiang Zhong, Guoqiang Huang, Hengbo Wang, Jinlong Guo","doi":"10.1111/pce.70180","DOIUrl":"https://doi.org/10.1111/pce.70180","url":null,"abstract":"<p><p>Cold stress is a critical environmental factor adversely affecting plant growth and development. As a tropical-origin crop constituting the primary global source of sucrose, sugarcane (Saccharum spp. hybrid) exhibits particular vulnerability to suboptimal temperature conditions, with chilling injury substantially compromising its yield potential. Despite its agricultural significance, the molecular mechanisms underlying cold acclimation in sugarcane remain poorly characterized. Here, we report a cold-repressed 1R-MYB gene, ScMYB7, from sugarcane, whose promoter (pro-ScMYB7) contains multiple cis-acting elements, including two cytosine-phosphate diester-guanine (CpG) islands. Bisulfite sequencing PCR (BSP) and qPCR results showed that low-temperature treatment increased the methylation level of the CpG islands in the promoter to reduce the transcription of the ScMYB7 gene. The outcomes of GUS enzyme activity measurement of the promoter also indicated that low-temperature treatment inhibits the promoter's transcriptional activity, and methylation inhibitors could alleviate this inhibition. By generating transgenic Arabidopsis lines overexpressing ScMYB7, ScMYB7's roles in regulating cold tolerance were investigated. We observed that the transgenic plants reduced cold tolerance, featured by a decreased survival rate after recovery, fluctuated physiological traits, and significantly lower expression levels of the C-repeat binding factor (CBF)-dependent pathway genes (AtCBFs, AtCOR15, and AtRD29A). Yeast one-hybrid assays demonstrated direct binding of ScMYB7 to the AtCBF1 promoter, while repression of sugarcane ScDREB1A occurred indirectly. Furthermore, the dual-luciferase reporter assay indicated that ScMYB7 was able to inhibit the expression of the AtCBF1 or ScDREB1A. Taken together, we propose a model in which ScMYB7 acts as a repressor of cold tolerance via the CBF-dependent pathway. Under low-temperature stress, increased methylation of the pro-ScMYB7 promoter reduces ScMYB7 expression, thereby alleviating its repression of sugarcane DREB/CBF-type transcription factors and enhancing cold adaptation.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
S-Methyl-l-Cysteine Sulfoxide: A Hidden Layer of Defences Against Herbivorous Insects in Brassicaceae. s -甲基-l-半胱氨酸亚砜:十字花科植物抵御草食性昆虫的隐藏屏障。
IF 6.3 1区 生物学
Plant, Cell & Environment Pub Date : 2025-09-12 DOI: 10.1111/pce.70179
Laura Bellec, Célia Le Maire, Nathalie Marnet, Chrystelle Paty, Anne-Marie Cortesero, Maxime R Hervé
{"title":"S-Methyl-l-Cysteine Sulfoxide: A Hidden Layer of Defences Against Herbivorous Insects in Brassicaceae.","authors":"Laura Bellec, Célia Le Maire, Nathalie Marnet, Chrystelle Paty, Anne-Marie Cortesero, Maxime R Hervé","doi":"10.1111/pce.70179","DOIUrl":"https://doi.org/10.1111/pce.70179","url":null,"abstract":"<p><p>Plants defend themselves against herbivorous insects through diverse morphological and biochemical traits. Non-protein amino acids (NPAAs) are an important component of the plant metabolome, although their defensive function remains largely unexplored. Here, we investigated the role of S-methyl-l-cysteine sulfoxide (SMCSO), a sulphur-containing NPAA accumulated in Brassicaceae, in mediating plant defence against herbivorous insects. SMCSO was quantified in inflorescences, leaves (young and old) and roots (primary and secondary) of 14 Brassicaceae species. Additionally, feeding tests on artificial substrates supplemented with physiological SMCSO levels were conducted with both generalist and specialist herbivorous insects feeding on one of the different plant parts studied. In line with the optimal defence theory, we found higher SMCSO levels in reproductive than vegetative tissues, and in young leaves and primary roots compared to old leaves and secondary roots, respectively. SMCSO also exerted a consistent phagodeterrent effect on generalist herbivores, while specialists showed variable responses from deterrence to stimulation. This study provides the first evidence of the influence of this compound on herbivore feeding behaviour, with effects depending on the diet breadth. These findings broaden our understanding of plant chemical defence complexity and highlight the ecological role of NPAAs in plant-insect interactions.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Transcription Factor MYB8 Positively Regulates Flavonoid Biosynthesis of Scutellaria baicalensis in Response to Drought Stress. 转录因子MYB8正调控黄芩黄酮合成对干旱胁迫的响应
IF 6.3 1区 生物学
Plant, Cell & Environment Pub Date : 2025-09-11 DOI: 10.1111/pce.70178
Chong Chen, Xiaofang Zhou, Bo Cao, Shan Feng, Tiantian Bin, Yali Zhang, Pufan Gao, Yumeng Lu, Xian Li, Lianjin Liu, Suying Hu, Bowen Zheng, Guishuang Li, Chengke Bai
{"title":"The Transcription Factor MYB8 Positively Regulates Flavonoid Biosynthesis of Scutellaria baicalensis in Response to Drought Stress.","authors":"Chong Chen, Xiaofang Zhou, Bo Cao, Shan Feng, Tiantian Bin, Yali Zhang, Pufan Gao, Yumeng Lu, Xian Li, Lianjin Liu, Suying Hu, Bowen Zheng, Guishuang Li, Chengke Bai","doi":"10.1111/pce.70178","DOIUrl":"https://doi.org/10.1111/pce.70178","url":null,"abstract":"<p><p>Drought stress dynamically reprograms specialised metabolism in medicinal plants. However, the transcriptional regulatory modules governing stress-adaptive metabolite synthesis remain poorly characterised. Here, we identified SbMYB8 as a drought-responsive transcription factor showing nuclear localisation and dose-dependent induction under drought in Scutellaria baicalensis. SbMYB8 activation triggered coordinated upregulation of six baicalin biosynthetic genes, elevating total baicalin and aglycones. Heterologous overexpression in Arabidopsis thaliana revealed SbMYB8's conserved regulatory function, driving anthocyanin accumulation (2.3-fold), flavonoid hyperproduction (5.8-fold), and developmental plasticity through enhanced lateral root proliferation. Specifically, we established the first stable S. baicalensis genetic transformation system, enabling tissue-specific dissection of SbMYB8 function. Transgenic OE-SbMYB8 lines exhibited root architectural remodelling (thickened primary roots, increased lateral root density) and root-specific flavonoid amplification (baicalin 1.8-fold; total flavonoids 3.5-fold), coupled with hierarchical induction of 12 pathway genes. Low-dose PEG (2.5%) synergised with SbMYB8 to transiently boost aglycone synthesis, whereas high-dose stress (5%) disrupted this coordination, suppressing biosynthetic machinery and metabolite yields. Mechanistically, yeast one-hybrid and dual-luciferase assays revealed SbMYB8 directly binds cis-elements in target promoters to orchestrate pathway activation. Based on the above results, we propose a SbMYB8-mediated \"drought perception - transcriptional activation - metabolic response\" network and provide transformative tools for precision breeding of stress-resilient medicinal plants.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic Regulation Differences of VRS Genes in the Development of Lateral Spikelets in Two-Rowed Barley. 二棱大麦侧穗发育中VRS基因的遗传调控差异
IF 6.3 1区 生物学
Plant, Cell & Environment Pub Date : 2025-09-11 DOI: 10.1111/pce.70183
Liping Shen, Zhiwen Sun, Yangyang Liu, Kuocheng Shen, Zhimin Wang, Botao Ye, Ziying Wang, Zifeng Guo
{"title":"Genetic Regulation Differences of VRS Genes in the Development of Lateral Spikelets in Two-Rowed Barley.","authors":"Liping Shen, Zhiwen Sun, Yangyang Liu, Kuocheng Shen, Zhimin Wang, Botao Ye, Ziying Wang, Zifeng Guo","doi":"10.1111/pce.70183","DOIUrl":"https://doi.org/10.1111/pce.70183","url":null,"abstract":"<p><p>The barley (Hordeum vulgare L.) spike consists of one central and two lateral spikelets at each rachis node. In two-rowed barley, only the central spikelet is fertile, the lateral spikelets also produce grain while in six-rowed barley. Five SIX-ROWED SPIKE genes (VRS1-5) have been identified as regulators of lateral spikelet fertility in barley, but the underlying genetic mechanisms of these VRS genes remain unclear. In this study, we conducted a detailed observation of the development process of the lateral spikelets in two-rowed barley and performed comparative transcriptome analysis to investigate gene expression differences between vrs1-5 mutants and wild-type spikelets. This revealed the differences in the downstream pathways regulated by the VRS genes in lateral spikelet development and the correlation of the effects of different VRS genes on lateral spikelet fertility. Using chromatin immunoprecipitation sequencing (ChIP-seq) and RNA sequencing (RNA-seq), we identified 213 direct downstream genes of VRS1, including those involved in energy metabolism, hormone pathways, and transcription factors. We also discovered that VRS1 directly binds to the D-class gene HvMADS13 to regulate spikelet fertility. Further analysis of the six-rowed barley accessions revealed that a 1 bp deletion in the C-terminus of VRS1 disrupts its ability to repress transcription, leading to fertility in the lateral spikelets.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Epigenetic Regulation of Agronomic Traits and Environmental Adaptability in Brassicas. 芸苔属植物农艺性状的表观遗传调控及环境适应性。
IF 6.3 1区 生物学
Plant, Cell & Environment Pub Date : 2025-09-11 DOI: 10.1111/pce.70177
Daolei Zhang, Yin Lu, Wei Ma, Jianjun Zhao
{"title":"The Epigenetic Regulation of Agronomic Traits and Environmental Adaptability in Brassicas.","authors":"Daolei Zhang, Yin Lu, Wei Ma, Jianjun Zhao","doi":"10.1111/pce.70177","DOIUrl":"https://doi.org/10.1111/pce.70177","url":null,"abstract":"<p><p>As essential sources of vegetables, oilseeds, and forage, Brassica crops exhibit complex epigenetic regulation mechanisms involving histone modifications, DNA modifications, RNA modifications, noncoding RNAs, and chromatin remodelling. The agronomic traits and environmental adaptability of crops are regulated by both genetic and epigenetic mechanisms, while epigenetic variation can affect plant phenotypes without changing gene sequences. Furthermore, the impact of epigenetic modifications on plant phenotype has accelerated the crop breeding process. This review highlights the epigenetic mechanisms underlying agronomic and stress-related traits in Brassica crops, while systematically identifying and categorising RNA modification-associated proteins within these species. We further propose an innovative strategy for improving Brassica crops yield through epigenome editing technology. Finally, we discuss the prospects and challenges for the future application of epigenetics-mediated crop breeding (epibreeding) strategies in Brassica crops.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sucrose-Induced Transcription Factor IjSUSIBA2 Fine-Tunes Growth Transition Through the IjSVL2-IjFLCL1 Module in Evergreen Irises. 蔗糖诱导的转录因子IjSUSIBA2通过IjSVL2-IjFLCL1模块调控常绿鸢尾的生长转变。
IF 6.3 1区 生物学
Plant, Cell & Environment Pub Date : 2025-09-10 DOI: 10.1111/pce.70166
Lingmei Shao, Tong Xu, Xiaobin Wang, Xiaoxuan Chen, Runlong Zhang, Ziming Ren, Yun Wu, Qinsong Yang, Cheng Luo, Jiaping Zhang, David P Horvath, Yiping Xia, Danqing Li
{"title":"Sucrose-Induced Transcription Factor IjSUSIBA2 Fine-Tunes Growth Transition Through the IjSVL2-IjFLCL1 Module in Evergreen Irises.","authors":"Lingmei Shao, Tong Xu, Xiaobin Wang, Xiaoxuan Chen, Runlong Zhang, Ziming Ren, Yun Wu, Qinsong Yang, Cheng Luo, Jiaping Zhang, David P Horvath, Yiping Xia, Danqing Li","doi":"10.1111/pce.70166","DOIUrl":"https://doi.org/10.1111/pce.70166","url":null,"abstract":"<p><p>Sugar metabolism is commonly implicated as crucial in the transition between growth and cessation during winter; however, its exact role remains elusive. The evergreen iris (Iris japonica) ceases growth in winter without entering endodormancy, yet it continues to sustain sugar metabolism and transport throughout the season. Here, we elucidate the mechanisms underlying the sugar-mediated growth transition-the shift between growth and cessation-in I. japonica through integrative physiological and transcriptional analyses. We investigated the function and transcriptional regulation of FLOWERING LOCUS C-LIKE 1 (IjFLCL1) and demonstrated that IjFLCL1 promotes growth resumption. Additionally, ALPHA-AMYLASE-LIKE 3 (IjAMY3), a starch degradation gene downstream of IjFLCL1, inhibits growth in I. japonica, potentially by modulating endogenous starch granule dynamics. Moreover, sugar-induced transcription factor SUGAR SIGNALING IN BARLEY 2 (IjSUSIBA2) activates IjFLCL1 expression. Conversely, IjSUSIBA2 directly represses the well-known dormancy mediator SHORT VEGETATIVE PHASE-LIKE 2 (IjSVL2), which in turn inhibits IjFLCL1 expression. IjSUSIBA2 may be induced by both exogenous and endogenous sugar signals, subsequently regulating downstream genes. Overall, our results suggest sugar molecules might serve as both signals and energy to regulate growth transition through IjFLCL1-mediated novel pathways. These insights carry valuable implications for the cultivation and breeding of perennials to withstand the challenges posed by climate change.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Integrated Physiological, Transcriptomic, and Metabolomic Analyses Reveal the Response Mechanisms of Selenium (Se) and Boron (B) Under Lead (Pb) Stress in Tobacco. 综合生理、转录组学和代谢组学分析揭示了烟草对铅(Pb)胁迫下硒(Se)和硼(B)的响应机制
IF 6.3 1区 生物学
Plant, Cell & Environment Pub Date : 2025-09-09 DOI: 10.1111/pce.70172
Lingling Jin, Qian Qiao, Long Yang, Yun Gao
{"title":"Integrated Physiological, Transcriptomic, and Metabolomic Analyses Reveal the Response Mechanisms of Selenium (Se) and Boron (B) Under Lead (Pb) Stress in Tobacco.","authors":"Lingling Jin, Qian Qiao, Long Yang, Yun Gao","doi":"10.1111/pce.70172","DOIUrl":"https://doi.org/10.1111/pce.70172","url":null,"abstract":"<p><p>Selenium and boron can alleviate lead (Pb) toxicity in plants, but their stress resistance mechanisms in tobacco remain unclear. The aim of this study was to investigate the effects of Se/B application on lead-induced oxidative stress, subcellular distribution, cell wall properties, and Pb accumulation. Additionally, a comprehensive analysis of transcriptomics and metabolomics data was conducted. Under Pb stress, the combined application of exogenous addition of Se, B and Se + B to a hydroponic system led to an increase in leaf biomass, promoted photosynthesis, increased antioxidant enzyme activity in tobacco, and significantly reduced the accumulation of Pb in the shoots of tobacco by downregulating the gene expression of the ABC transporter and upregulating the expression of aquaporin (AQP). Additionally, the application of Se and B increased the activity of the phenylpropanoid and glutathione metabolism pathways, promoting the synthesis of secondary metabolites such as coumarin, chlorogenic acid, ferulaldehyde, and (5-l-glutamyl)-l-amino acids. This, in turn, increased the tolerance of tobacco plants to Pb stress. Overall, our research findings provide new theoretical evidence regarding the roles of Se and B in alleviating Pb accumulation and offer potential strategies for the use of Se and B in the remediation of Pb-contaminated soils in agricultural production.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CRISPR RNP-Mediated Transgene-Free Genome Editing in Plants: Advances, Challenges and Future Directions for Tree Species. CRISPR rnp介导的植物无转基因基因组编辑:进展、挑战和未来方向
IF 6.3 1区 生物学
Plant, Cell & Environment Pub Date : 2025-09-09 DOI: 10.1111/pce.70176
Muthusamy Ramakrishnan, Rashmi Kaul, Anket Sharma, Zishan Ahmad, Venkatesan Vijayakanth, Krishnamurthi Keerthana, Zhipeng Gao, Mingbing Zhou, Qiang Wei
{"title":"CRISPR RNP-Mediated Transgene-Free Genome Editing in Plants: Advances, Challenges and Future Directions for Tree Species.","authors":"Muthusamy Ramakrishnan, Rashmi Kaul, Anket Sharma, Zishan Ahmad, Venkatesan Vijayakanth, Krishnamurthi Keerthana, Zhipeng Gao, Mingbing Zhou, Qiang Wei","doi":"10.1111/pce.70176","DOIUrl":"https://doi.org/10.1111/pce.70176","url":null,"abstract":"<p><p>CRISPR ribonucleoprotein (RNP)-mediated genome editing offers a transgene-free platform for precise genetic modification in diverse herbaceous and tree species, including rice, wheat, apple, poplar, oil palm, rubber tree and grapevine. However, its application in woody plants faces distinct challenges, notably inefficient delivery and regeneration difficulties, particularly in species such as bamboo. While some of these issues also occur in herbaceous plants, they are often significantly more complex in woody species due to factors such as intricate cell wall architecture, widespread recalcitrant genotypes and inherent limitations of current delivery platforms. This review presents the first in-depth, critical re-evaluation of recent advancements in RNP-mediated editing in woody plants, highlighting these obstacles that warrant focused attention. Unlike plasmid-based CRISPR systems, RNP editing utilises Cas9/Cas12a protein-guide RNA complexes without integrating foreign DNA. This enables a DNA-free editing strategy that simplifies regulatory approval and minimises off-target effects due to the transient presence and rapid degradation of RNPs within plant cells. While PEG-mediated protoplast transfection and particle bombardment remain the primary reported methods for RNP delivery in trees, we evaluate promising alternative strategies such as lipofection, electroporation, cell-penetrating peptides and nanoparticle-based systems for targeted RNP delivery. Despite their promise, these advanced methods remain largely untested in woody species. Finally, we outline future research directions, including the development of tree-specific RNP delivery systems and regeneration protocols to enhance efficiency and minimise cytotoxicity. These innovations are essential for unlocking the full potential of RNP-mediated genome editing in long-lived tree species. This review provides a focused and timely roadmap for expanding the application of RNP technology across diverse woody plants.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Repeated Soil Droughts Induce Differential Responses Across Leaflets of a Compound Leaf. 重复土壤干旱诱导复叶叶片间的差异响应。
IF 6.3 1区 生物学
Plant, Cell & Environment Pub Date : 2025-09-08 DOI: 10.1111/pce.70175
Tomasz Hura, Katarzyna Hura, Agnieszka Ostrowska, Karolina Urban, Kinga Dziurka, Bożena Pawłowska
{"title":"Repeated Soil Droughts Induce Differential Responses Across Leaflets of a Compound Leaf.","authors":"Tomasz Hura, Katarzyna Hura, Agnieszka Ostrowska, Karolina Urban, Kinga Dziurka, Bożena Pawłowska","doi":"10.1111/pce.70175","DOIUrl":"https://doi.org/10.1111/pce.70175","url":null,"abstract":"","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信