Yi Yang, Jian Luo, Jingyi Feng, Huihui Hu, Jianke Xiao, Chengqiang Ding, Vinay Nangia, Yang Liu
{"title":"根源反式玉米蛋白型细胞分裂素通过调节能量代谢提高水稻幼苗的抗寒性。","authors":"Yi Yang, Jian Luo, Jingyi Feng, Huihui Hu, Jianke Xiao, Chengqiang Ding, Vinay Nangia, Yang Liu","doi":"10.1111/pce.70190","DOIUrl":null,"url":null,"abstract":"<p><p>Rice seedlings are highly sensitive to low-temperature stress. Cytokinins are important endogenous signalling molecules in plants and play a critical role in regulating stress responses. However, the mechanism by which cytokinins mediate cold stress responses in rice seedlings remains unclear. In this study, we employed cold-tolerant and cold-sensitive rice cultivars, cytokinin-defective mutants and exogenous cytokinin supplementation to elucidate the mechanisms underlying cytokinin-mediated chilling adaptation. First, we compared the transcriptomic and metabolomic profiles of a cold-tolerant cultivar (HY73) and a cold-sensitive cultivar (WFY286) under low-temperature treatment (11°C for 8 h). The results revealed that cytokinins, along with energy metabolic pathways such as glycolysis and the tricarboxylic acid cycle, are closely associated with cold tolerance in rice seedlings. Compared with WFY286, HY73 presented higher levels of root-derived trans-zeatin (tZ)-type cytokinins in leaves, increased energy metabolism, elevated ATP content and increased energy charge. Furthermore, the tZ-type cytokinins transport-deficient mutant abcg18 presented reduced cold tolerance, lower energy metabolic activity and decreased ATP and energy charge levels, indicating that the transport of tZ-type cytokinins is crucial for cold stress responses. Leaf spraying with tZ significantly improved the energy metabolism and cold tolerance of WFY286 and abcg18. Taken together, our findings suggest that root-derived tZ-type cytokinins enhance low-temperature adaptation in rice seedlings by promoting energy metabolism and maintaining cellular energy homoeostasis. This study provides a theoretical basis for improving cold resistance in rice through manipulation of cytokinin signalling pathways.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3000,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Root-Derived Trans-Zeatin-Type Cytokinins Increase Cold Tolerance in Rice Seedlings by Regulating Energy Metabolism.\",\"authors\":\"Yi Yang, Jian Luo, Jingyi Feng, Huihui Hu, Jianke Xiao, Chengqiang Ding, Vinay Nangia, Yang Liu\",\"doi\":\"10.1111/pce.70190\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Rice seedlings are highly sensitive to low-temperature stress. Cytokinins are important endogenous signalling molecules in plants and play a critical role in regulating stress responses. However, the mechanism by which cytokinins mediate cold stress responses in rice seedlings remains unclear. In this study, we employed cold-tolerant and cold-sensitive rice cultivars, cytokinin-defective mutants and exogenous cytokinin supplementation to elucidate the mechanisms underlying cytokinin-mediated chilling adaptation. First, we compared the transcriptomic and metabolomic profiles of a cold-tolerant cultivar (HY73) and a cold-sensitive cultivar (WFY286) under low-temperature treatment (11°C for 8 h). The results revealed that cytokinins, along with energy metabolic pathways such as glycolysis and the tricarboxylic acid cycle, are closely associated with cold tolerance in rice seedlings. Compared with WFY286, HY73 presented higher levels of root-derived trans-zeatin (tZ)-type cytokinins in leaves, increased energy metabolism, elevated ATP content and increased energy charge. Furthermore, the tZ-type cytokinins transport-deficient mutant abcg18 presented reduced cold tolerance, lower energy metabolic activity and decreased ATP and energy charge levels, indicating that the transport of tZ-type cytokinins is crucial for cold stress responses. Leaf spraying with tZ significantly improved the energy metabolism and cold tolerance of WFY286 and abcg18. Taken together, our findings suggest that root-derived tZ-type cytokinins enhance low-temperature adaptation in rice seedlings by promoting energy metabolism and maintaining cellular energy homoeostasis. This study provides a theoretical basis for improving cold resistance in rice through manipulation of cytokinin signalling pathways.</p>\",\"PeriodicalId\":222,\"journal\":{\"name\":\"Plant, Cell & Environment\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":6.3000,\"publicationDate\":\"2025-09-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant, Cell & Environment\",\"FirstCategoryId\":\"2\",\"ListUrlMain\":\"https://doi.org/10.1111/pce.70190\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant, Cell & Environment","FirstCategoryId":"2","ListUrlMain":"https://doi.org/10.1111/pce.70190","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Root-Derived Trans-Zeatin-Type Cytokinins Increase Cold Tolerance in Rice Seedlings by Regulating Energy Metabolism.
Rice seedlings are highly sensitive to low-temperature stress. Cytokinins are important endogenous signalling molecules in plants and play a critical role in regulating stress responses. However, the mechanism by which cytokinins mediate cold stress responses in rice seedlings remains unclear. In this study, we employed cold-tolerant and cold-sensitive rice cultivars, cytokinin-defective mutants and exogenous cytokinin supplementation to elucidate the mechanisms underlying cytokinin-mediated chilling adaptation. First, we compared the transcriptomic and metabolomic profiles of a cold-tolerant cultivar (HY73) and a cold-sensitive cultivar (WFY286) under low-temperature treatment (11°C for 8 h). The results revealed that cytokinins, along with energy metabolic pathways such as glycolysis and the tricarboxylic acid cycle, are closely associated with cold tolerance in rice seedlings. Compared with WFY286, HY73 presented higher levels of root-derived trans-zeatin (tZ)-type cytokinins in leaves, increased energy metabolism, elevated ATP content and increased energy charge. Furthermore, the tZ-type cytokinins transport-deficient mutant abcg18 presented reduced cold tolerance, lower energy metabolic activity and decreased ATP and energy charge levels, indicating that the transport of tZ-type cytokinins is crucial for cold stress responses. Leaf spraying with tZ significantly improved the energy metabolism and cold tolerance of WFY286 and abcg18. Taken together, our findings suggest that root-derived tZ-type cytokinins enhance low-temperature adaptation in rice seedlings by promoting energy metabolism and maintaining cellular energy homoeostasis. This study provides a theoretical basis for improving cold resistance in rice through manipulation of cytokinin signalling pathways.
期刊介绍:
Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.