根源反式玉米蛋白型细胞分裂素通过调节能量代谢提高水稻幼苗的抗寒性。

IF 6.3 1区 生物学 Q1 PLANT SCIENCES
Yi Yang, Jian Luo, Jingyi Feng, Huihui Hu, Jianke Xiao, Chengqiang Ding, Vinay Nangia, Yang Liu
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引用次数: 0

摘要

水稻幼苗对低温胁迫非常敏感。细胞分裂素是植物体内重要的内源信号分子,在调控胁迫反应中起重要作用。然而,细胞分裂素介导水稻幼苗冷胁迫反应的机制尚不清楚。在这项研究中,我们利用耐冷和冷敏感的水稻品种、细胞分裂素缺陷突变体和外源细胞分裂素补充来阐明细胞分裂素介导的低温适应机制。首先,我们比较了耐冷品种(HY73)和冷敏感品种(WFY286)在低温(11°C, 8 h)处理下的转录组学和代谢组学特征。结果表明,细胞分裂素和糖酵解、三羧酸循环等能量代谢途径与水稻幼苗的耐寒性密切相关。与WFY286相比,HY73叶片中根源反式玉米素(tZ)型细胞分裂素水平升高,能量代谢增加,ATP含量升高,能量电荷增加。此外,z型细胞分裂素运输缺陷突变体abcg18的耐寒性降低,能量代谢活性降低,ATP和能量电荷水平降低,表明z型细胞分裂素的运输对冷胁迫反应至关重要。喷施tZ显著提高了WFY286和abcg18的能量代谢和耐寒性。综上所述,我们的研究结果表明,根系来源的z型细胞分裂素通过促进能量代谢和维持细胞能量平衡来增强水稻幼苗的低温适应性。本研究为通过调控细胞分裂素信号通路提高水稻抗寒性提供了理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Root-Derived Trans-Zeatin-Type Cytokinins Increase Cold Tolerance in Rice Seedlings by Regulating Energy Metabolism.

Rice seedlings are highly sensitive to low-temperature stress. Cytokinins are important endogenous signalling molecules in plants and play a critical role in regulating stress responses. However, the mechanism by which cytokinins mediate cold stress responses in rice seedlings remains unclear. In this study, we employed cold-tolerant and cold-sensitive rice cultivars, cytokinin-defective mutants and exogenous cytokinin supplementation to elucidate the mechanisms underlying cytokinin-mediated chilling adaptation. First, we compared the transcriptomic and metabolomic profiles of a cold-tolerant cultivar (HY73) and a cold-sensitive cultivar (WFY286) under low-temperature treatment (11°C for 8 h). The results revealed that cytokinins, along with energy metabolic pathways such as glycolysis and the tricarboxylic acid cycle, are closely associated with cold tolerance in rice seedlings. Compared with WFY286, HY73 presented higher levels of root-derived trans-zeatin (tZ)-type cytokinins in leaves, increased energy metabolism, elevated ATP content and increased energy charge. Furthermore, the tZ-type cytokinins transport-deficient mutant abcg18 presented reduced cold tolerance, lower energy metabolic activity and decreased ATP and energy charge levels, indicating that the transport of tZ-type cytokinins is crucial for cold stress responses. Leaf spraying with tZ significantly improved the energy metabolism and cold tolerance of WFY286 and abcg18. Taken together, our findings suggest that root-derived tZ-type cytokinins enhance low-temperature adaptation in rice seedlings by promoting energy metabolism and maintaining cellular energy homoeostasis. This study provides a theoretical basis for improving cold resistance in rice through manipulation of cytokinin signalling pathways.

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来源期刊
Plant, Cell & Environment
Plant, Cell & Environment 生物-植物科学
CiteScore
13.30
自引率
4.10%
发文量
253
审稿时长
1.8 months
期刊介绍: Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.
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