拟南芥denadd酶DXO1调控植物免疫应答

IF 6.3 1区 生物学 Q1 PLANT SCIENCES
Anna Golisz-Mocydlarz, Monika Zakrzewska-Placzek, Michal Krzyszton, Nataliia Diachenko, Justyna Piotrowska, Wiktoria Kalbarczyk, Agnieszka Marasek-Ciolakowska, Joanna Kufel
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引用次数: 0

摘要

DXO1是拟南芥中唯一的DXO同源物,由于其植物特异性,表现出较强的denadd酶活性,但在5' QC中没有明显作用。尽管它对植物RNA代谢有重要贡献,但DXO1酶活性对细胞过程的直接影响却令人惊讶地有限。值得注意的是,到目前为止,在dxo1突变株中观察到的大多数分子和形态变化都依赖于该蛋白的植物特异性n端结构域。我们研究了DXO1在应对生物胁迫中的作用,特别是它对丁香假单胞菌的敏感性。研究结果揭示了DXO1酶活性在植物免疫应答中的重要性。我们观察到,表达无催化活性或缺乏n端结构域的DXO1 -2基因敲除突变体和转基因DXO1 -2细胞系对Pst的抗性增强,并伴有关键发病标志物表达的显著变化。此外,PAMPs elf18和flg22还能强烈诱导植物免疫的其他标志物,如胼胝质沉积和活性氧的产生。这些结果表明,DXO1的n端结构域及其催化位点都参与了植物免疫的调控。这是首次发现DXO1酶活性参与植物生理。此外,我们的分析表明,dxo1-2突变改变了一大组防御相关基因的表达,影响了mrna的稳定性,并延迟了MAP激酶的激活。因此,我们假设DXO1蛋白在转录和转录后水平上解除了对Pst感染的防御。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Arabidopsis deNADding Enzyme DXO1 Modulates the Plant Immunity Response.

DXO1, the only DXO homolog in Arabidopsis, due to its plant-specific features, exhibits strong deNADding enzymatic activity but has no apparent role in 5' QC. Despite its important contribution to plant RNA metabolism, the direct impact of DXO1 enzymatic activity on cellular processes appears surprisingly limited. Notably, most molecular and morphological changes observed so far in dxo1 mutant plants depended on the plant-specific N-terminal domain of the protein. Our investigation into the role of DXO1 in response to biotic stress, specifically its susceptibility to Pseudomonas syringae pv. tomato DC3000 infection, unexpectedly revealed the importance of DXO1 enzymatic activity in the plant immune response. We observed that dxo1-2 knockout mutant and transgenic dxo1-2 lines expressing a DXO1 variant either catalytically inactive or lacking the N-terminal domain exhibited enhanced resistance to Pst, accompanied by marked changes in the expression of key pathogenesis markers. Also, other markers of plant immunity, such as callose deposition and production of reactive oxygen species, were strongly induced by PAMPs elf18 and flg22. These results strongly suggest that both DXO1 features, the N-terminal domain and its catalytic site, contribute to the regulation of plant immunity. This is the first observation revealing the involvement of DXO1 enzymatic activity in plant physiology. Moreover, our analyses showed that dxo1-2 mutation altered the expression of a large group of defense-related genes, affected the stability of mRNAs, and delayed the activation of MAP kinases. Therefore, we postulate that DXO1 protein deregulates defense against Pst infection at both the transcriptional and posttranscriptional levels.

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来源期刊
Plant, Cell & Environment
Plant, Cell & Environment 生物-植物科学
CiteScore
13.30
自引率
4.10%
发文量
253
审稿时长
1.8 months
期刊介绍: Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.
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