Mycobiology最新文献_第6页

Two New Species of the Family Acarosporaceae from South Korea. 标题韩国粉孢科二新种

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-09-07 eCollection Date: 2023-01-01 DOI: 10.1080/12298093.2023.2249693

Jung Shin Park, Young-Nam Kwag, Sang-Kuk Han, Soon-Ok Oh

{"title":"Two New Species of the Family Acarosporaceae from South Korea.","authors":"Jung Shin Park, Young-Nam Kwag, Sang-Kuk Han, Soon-Ok Oh","doi":"10.1080/12298093.2023.2249693","DOIUrl":"10.1080/12298093.2023.2249693","url":null,"abstract":"Acarosporaceae is a crustose lichen and is known as a species that has more than 50 multi-spores, and has hyaline spores. Those taxa are often found in rock and soil in mountain areas or coastal regions in Korea, and very diverse forms and species are known. However, after an overall genetic phylogenetic analysis of carbonized ascomata in 2015, species consisting only of the morphological base are newly divided, and several species of Acarosporaceae in Korea are also being discovered in this situation. As a result of analysis using internal transcribed spacer (ITS) and nuLSU gene analysis, Korean species belonged to Acarospora and Sarcogyne clade, and Acarospora classified as the Acarospora clade was mixed with the Polysporina group and the Sarcogyne clade is mixed with the Acarospora. We identified two new species (Acarospora beangnokdamensis J. S. Park & S. O. Oh, sp. nov., Sarcogyne jejuensis J. S. Park & S. O. Oh, sp. nov.) through morphological, molecular, and secondary metabolite substance and found one new record (Sarcogyne oceanica K. Knudsen & Kocourk). We have made a classification key for Acarospora and Sarcogyne in Korea and reported all information together here.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10791086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45449210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 修正

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-08-03 eCollection Date: 2023-01-01 DOI: 10.1080/12298093.2023.2239652

引用次数: 0

Neocucurbitaria chlamydospora sp. nov.: A Novel Species of the Family Cucurbitariaceae Isolated from a Stink Bug in Korea. Neocucurbitaria chlamydospora sp：从韩国臭虫中分离出的葫芦科新物种。

IF 1.6 4区生物学

Mycobiology Pub Date : 2023-05-12 eCollection Date: 2023-01-01 DOI: 10.1080/12298093.2023.2203973

Soo-Min Hong, Kallol Das, Seong-Keun Lim, Sang Jae Suh, Seung-Yeol Lee, Hee-Young Jung

{"title":"Neocucurbitaria chlamydospora sp. nov.: A Novel Species of the Family Cucurbitariaceae Isolated from a Stink Bug in Korea.","authors":"Soo-Min Hong, Kallol Das, Seong-Keun Lim, Sang Jae Suh, Seung-Yeol Lee, Hee-Young Jung","doi":"10.1080/12298093.2023.2203973","DOIUrl":"10.1080/12298093.2023.2203973","url":null,"abstract":"The fungal strain KNUF-22-18B, belonging to Cucurbitariaceae, was discovered from a stink bug (Hygia lativentris) during the investigation of insect microbiota in Chungnam Province, South Korea. The colonies of the strain KNUF-22-18B were wooly floccose, white to brown in the center on oatmeal agar (OA), and the colonies were buff, margin even, and colorless, reverse white to yellowish toward the center on malt extract agar (MEA). The strain KNUF-22-18B produced pycnidia after 60 days of culturing on potato dextrose agar, but pycnidia were not observed on OA. On the contrary, N. keratinophila CBS 121759T abundantly formed superficial pycnidia on OA and MEA after a few days. The strain KNUF-22-18B produced chlamydospores subglobose to globose, mainly in the chain, with a small diameter of 4.4-8.8 μm. At the same time, N. keratinophila CBS 121759T displayed a globose terminal with a diameter of 8-10 μm. A multilocus phylogeny using the internal transcribed spacer regions, 28S rDNA large subunit, β-tubulin, and RNA polymerase II large subunit genes further validated the uniqueness of the strain. The detailed description and illustration of the proposed species as Neocucurbitaria chlamydospora sp. nov. from Korea was strongly supported by molecular phylogeny.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ee/6a/TMYB_51_2203973.PMC10288907.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10073191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity and community structure of ectomycorrhizal mycorrhizal fungi in roots and rhizosphere soil of Abies koreana and Taxus cuspidata in Mt. Halla. 哈拉山韩国杉和紫杉根系和根瘤土壤中外生菌根真菌的多样性和群落结构。

IF 1.6 4区生物学

Mycobiology Pub Date : 2023-01-04 eCollection Date: 2022-01-01 DOI: 10.1080/12298093.2022.2161974

Ji-Eun Lee, Ahn-Heum Eom

{"title":"Diversity and community structure of ectomycorrhizal mycorrhizal fungi in roots and rhizosphere soil of Abies koreana and Taxus cuspidata in Mt. Halla.","authors":"Ji-Eun Lee, Ahn-Heum Eom","doi":"10.1080/12298093.2022.2161974","DOIUrl":"10.1080/12298093.2022.2161974","url":null,"abstract":"In this study, the roots and rhizosphere soil of Abies koreana and Taxus cuspidata were collected from sites at two different altitudes on Mt. Halla. Ectomycorrhizal fungi (EMF) were identified by Illumina MiSeq sequencing. The proportion of EMF from the roots was 89% in A. koreana and 69% in T. cuspidata. Among EMF in rhizosphere soils, the genus Russula was the most abundant in roots of A. koreana (p < 0.05). The altitude did not affect the biodiversity of EMF communities but influenced fungal community composition. However, the host plants had the most significant effect on EMF communities. The result of the EMF community analysis showed that even if the EMF were isolated from the same altitudes, the EMF communities differed according to the host plant. The community similarity index of EMF in the roots of A. koreana was higher than that of T. cuspidata (p < 0.05). The results show that both altitude and host plants influenced the structure of EMF communities. Conifers inhabiting harsh sub-alpine environments rely strongly on symbiotic relationships with EMF. A. koreana is an endangered species with a higher host specificity of EMF and climate change vulnerability than T. cuspidata. This study provides insights into the EMF communities, which are symbionts of A. koreana, and our critical findings may be used to restore A. koreana.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c7/71/TMYB_50_2161974.PMC9848229.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10601998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taxonomy of Botryotrichum luteum sp. nov. based on Morphology and Phylogeny Isolated from Soil in Korea. 基于形态和系统发育的韩国黄体芽孢霉的分类。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2192613

Jung-Joo Ryu, Kallol Das, Seong-Keun Lim, Soo-Min Hong, Seung-Yeol Lee, Hee-Young Jung

{"title":"Taxonomy of Botryotrichum luteum sp. nov. based on Morphology and Phylogeny Isolated from Soil in Korea.","authors":"Jung-Joo Ryu, Kallol Das, Seong-Keun Lim, Soo-Min Hong, Seung-Yeol Lee, Hee-Young Jung","doi":"10.1080/12298093.2023.2192613","DOIUrl":"https://doi.org/10.1080/12298093.2023.2192613","url":null,"abstract":"In this study, a fungal strain KNUF-22-025 belonging to the genus Botryotrichum was isolated from the soil in Korea. The cultural and morphological characteristics of this strain differed from those of closely related species. On malt extract agar, strain KNUF-22-025 showed slower growth than most of the related species, except B. domesticum. The conidia size (9.6-21.1 × 9.9-18.4 µm) of strain KNUF-22-025 was larger than those of B. piluliferum, B. domesticum, and B. peruvianum but smaller than those of B. atrogriseum and B. iranicum. Conidiophores in strain KNUF-22-025 (137 µm) were longer than those in other closely related species but shorter than those in B. atrogriseum. Multi-locus analysis of molecular markers, such as ITS, 28S ribosomal DNA, RBP2, and TUB2 revealed that strain KNUF-22-025 was distinct from other Botryotrichum species. Thus, this strain is proposed as a novel species based on morphological characteristics along with molecular phylogeny and named Botryotrichum luteum sp. nov.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/78/46/TMYB_51_2192613.PMC10142302.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9397578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Culture Conditions for Mycelial Growth and Anti-Cancer Properties of Termitomyces. 白蚁菌丝生长的培养条件及抗癌特性研究。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2187614

Suphachai Tharavecharak, Corina N D'Alessandro-Gabazza, Masaaki Toda, Taro Yasuma, Taku Tsuyama, Ichiro Kamei, Esteban C Gabazza

{"title":"Culture Conditions for Mycelial Growth and Anti-Cancer Properties of Termitomyces.","authors":"Suphachai Tharavecharak, Corina N D'Alessandro-Gabazza, Masaaki Toda, Taro Yasuma, Taku Tsuyama, Ichiro Kamei, Esteban C Gabazza","doi":"10.1080/12298093.2023.2187614","DOIUrl":"https://doi.org/10.1080/12298093.2023.2187614","url":null,"abstract":"Termitomyces sp. that grow in symbiosis with fungus-farming Termites have medicinal properties. However, they are rare in nature, and their artificial culture is challenging. The expression of AXL receptor tyrosine kinase and immune checkpoint molecules favor the growth of cancer cells. The study evaluated the optimal conditions for the artificial culture of Termitomyces and their inhibitory activity on AXL and immune checkpoint molecules in lung adenocarcinoma and melanoma cell lines. The culture of 45 strains of Termitomyces was compared. Five strains with marked growth rates were selected. Four of the selected strains form a single cluster by sequence analysis. The mycelium of 4 selected strains produces more fungal mass in potato dextrose broth than in a mixed media. The bark was the most appropriate solid substrate for Termitomyces mycelia culture. The mycelium of all five selected strains showed a higher growth rate under normal CO2 conditions. The culture broth, methanol, and ethyl acetate of one selected strain (T-120) inhibited the mRNA relative expression of AXL receptor tyrosine kinase and immune checkpoint molecules in cancer cell lines. Overall, these results suggest the potential usefulness of Termitomyces extracts as a co-adjuvant therapy in malignant diseases.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b2/43/TMYB_51_2187614.PMC10142329.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9392601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Steroid Components of Marine-Derived Fungal Strain Penicillium levitum N33.2 and Their Biological Activities. 海洋源真菌利维青霉N33.2甾体成分及其生物活性研究

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2248717

Chi K Hoang, Cuong H Le, Dat T Nguyen, Hang T N Tran, Chinh V Luu, Huong M Le, Ha T H Tran

{"title":"Steroid Components of Marine-Derived Fungal Strain Penicillium levitum N33.2 and Their Biological Activities.","authors":"Chi K Hoang, Cuong H Le, Dat T Nguyen, Hang T N Tran, Chinh V Luu, Huong M Le, Ha T H Tran","doi":"10.1080/12298093.2023.2248717","DOIUrl":"https://doi.org/10.1080/12298093.2023.2248717","url":null,"abstract":"Genus Penicillium comprising the most important and extensively studied fungi has been well-known as a rich source of secondary metabolites. Our study aimed to analyze and investigate biological activities, including in vitro anti-cancer, anti-inflammatory and anti-diabetic properties, of metabolites from a marine-derived fungus belonging to P. levitum. The chemical compounds in the culture broth of P. levitum strain N33.2 were extracted with ethyl acetate. Followingly, chemical analysis of the extract leaded to the isolation of three ergostane-type steroid components, namely cerevisterol (1), ergosterol peroxide (2), and (3β,5α,22E)-ergosta-6,8(14),22-triene-3,5-diol (3). Among these, (3) was the most potent cytotoxic against human cancer cell lines Hep-G2, A549 and MCF-7 with IC50 values of 2.89, 18.51, and 16.47 µg/mL, respectively, while the compound (1) showed no significant effect against tested cancer cells. Anti-inflammatory properties of purified compounds were evaluated based on NO-production in LPS-induced murine RAW264.7 macrophages. As a result, tested compounds performed diverse inhibitory effects on NO production by the macrophages, with the most significant inhibition rate of 81.37 ± 1.35% at 25 µg/mL by the compound (2). Interestingly, compounds (2) and (3) exhibited inhibitory activities against pancreatic lipase and α-glucosidase enzymes in vitro assays. Our study brought out new data concerning the chemical properties and biological activities of isolated steroids from a P. levitum fungus.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/20/0e/TMYB_51_2248717.PMC10498798.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10261171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Cell Wall Integrity MAP Kinase Signaling Pathway Is Required for Development, Pathogenicity, and Stress Adaption of the Pepper Anthracnose Fungus Colletotrichum scovillei. 细胞壁完整性MAP激酶信号通路是辣椒炭疽病真菌炭疽菌发育、致病性和胁迫适应所必需的。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2220171

Teng Fu, Sung Wook Kang, Yong-Won Song, Kyoung Su Kim

{"title":"The Cell Wall Integrity MAP Kinase Signaling Pathway Is Required for Development, Pathogenicity, and Stress Adaption of the Pepper Anthracnose Fungus Colletotrichum scovillei.","authors":"Teng Fu, Sung Wook Kang, Yong-Won Song, Kyoung Su Kim","doi":"10.1080/12298093.2023.2220171","DOIUrl":"https://doi.org/10.1080/12298093.2023.2220171","url":null,"abstract":"The cell wall integrity (CWI) signaling pathway plays important roles in the dissemination and infection of several plant pathogenic fungi. However, its roles in the pepper fruit anthracnose fungus Colletotrichum scovillei remain uninvestigated. In this study, the major components of the CWI signaling pathway-CsMCK1 (MAPKKK), CsMKK1 (MAPKK), and CsMPS1 (MAPK)-were functionally characterized in C. scovillei via homology-dependent gene replacement. The ΔCsmck1, ΔCsmkk1, and ΔCsmps1 mutants showed impairments in fungal growth, conidiation, and tolerance to CWI and salt stresses. Moreover, ΔCsmck1, ΔCsmkk1, and ΔCsmps1 failed to develop anthracnose disease on pepper fruits due to defects in appressorium formation and invasive hyphae growth. These results suggest that CsMCK1, CsMKK1, and CsMPS1 play important roles in mycelial growth, conidiation, appressorium formation, plant infection, and stress adaption of C. scovillei. These findings will contribute to a better understanding of the roles of the CWI signaling pathway in the development of pepper fruit anthracnose disease.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/62/1e/TMYB_51_2220171.PMC10288896.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9714406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Clarification of Auricularia heimuer Strains Bred and Cultivated in Korea Using the ITS and IGS1 rDNA Region Sequences. 利用ITS和IGS1 rDNA区域序列对韩国黑木耳选育品系的遗传澄清

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2182024

Nitesh Pant, HyeongJin Noh, Won-Ho Lee, Seong Hwan Kim

{"title":"Genetic Clarification of Auricularia heimuer Strains Bred and Cultivated in Korea Using the ITS and IGS1 rDNA Region Sequences.","authors":"Nitesh Pant, HyeongJin Noh, Won-Ho Lee, Seong Hwan Kim","doi":"10.1080/12298093.2023.2182024","DOIUrl":"https://doi.org/10.1080/12298093.2023.2182024","url":null,"abstract":"Auricularia is one of the broadly cultivated edible mushrooms in Korea. Most of the Korean Auricularia strains used for cultivation and breeding are known as A. auricula-judae. Recently, this species has been reported to belong to a species complex. Therefore, this study was carried out to genetically clarify the bred and cultivated Korean A. auricula-judae strains. The internal transcribed spacer (ITS) and IGS1 rDNA region sequences were determined from 10 A. auricula-judae strains by PCR and sequencing. Variation in the nucleotide sequence and sequence length of the two rDNA regions were found among the seven A. auricula-judae strains. A maximum-likelihood (ML) phylogenetic tree based on the ITS sequences clearly placed all the 10 Korean A. auricula-judae strains in the A. heimuer clade of the A. auricula-judae complex. A. heimuer is diverged from A. auricula-judae. An ML phylogenetic tree based on the IGS1 sequences revealed the close relationship between Korean A. heimuer strains to Chinese A. heimuer strains. But each strain could be distinguishable by the IGS1 sequence. Furthermore, progeny strains in the seven Korean strains could be differentiated from their parental strains by the IGS1 sequence based phylogenetic tree. Our results are expected to be used to complement the distinction of domestic Auricularia cultivars.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/bf/b0/TMYB_51_2182024.PMC10142399.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9392602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Re-Examination of Several Elsinoë Species Reported from Japan. 日本报道的若干Elsinoë种的再检验。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2219049

Anysia Hedy Ujat, Tsuyoshi Ono, Yukako Hattori, Chiharu Nakashima

引用次数: 0