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A Novel Phaeoacremonium Species Isolated from Galls on the Chinese Magnolia-Vine (Schisandra chinensis) in Korea. 从中国木兰花(Schisandra chinensis)瘿中分离的一种新的Phaeoacremonium。
IF 1.6 4区 生物学
Mycobiology Pub Date : 2025-02-14 eCollection Date: 2025-01-01 DOI: 10.1080/12298093.2024.2438463
Seong-Keun Lim, Mohammad Hamizan Azmi, Min-Ki Kim, Seung-Han Kim, Seung-Yeol Lee, Hee-Young Jung
{"title":"A Novel <i>Phaeoacremonium</i> Species Isolated from Galls on the Chinese Magnolia-Vine (<i>Schisandra chinensis</i>) in Korea.","authors":"Seong-Keun Lim, Mohammad Hamizan Azmi, Min-Ki Kim, Seung-Han Kim, Seung-Yeol Lee, Hee-Young Jung","doi":"10.1080/12298093.2024.2438463","DOIUrl":"10.1080/12298093.2024.2438463","url":null,"abstract":"<p><p>The fungal strain KNUF-24-9L1a, belonging to the genus <i>Phaeoacremonium</i>, was isolated from gall-midge (<i>Lasioptera</i> sp.; Diptera: Cecidomyiidae) larvae and their galleries on a Chinese magnolia-vine (<i>Schisandra chinensis</i>) sample collected in Mungyeong-si, Gyeongbuk province, Korea. Phylogenetic analyses based on concatenated nucleotide sequences of the beta-tubulin and actin genes revealed that the strain clustered with <i>Phaeoacremonium</i> species but occupied a distinct phylogenetic position. Morphological differences between strain KNUF-24-9L1a and closely related species were also observed. In this study, we provide detailed descriptions, illustrations, and discussions of the morphological and phylogenetic analyses of closely related species to support the novelty of this isolated species. The phylogenetic and morphological evidence suggests that strain KNUF-24-9L1a represents a novel species within the genus <i>Phaeoacremonium</i>, which we have designated this species as <i>Phaeoacremonium schisandrae</i> sp. nov.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"53 2","pages":"243-249"},"PeriodicalIF":1.6,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Digital Analysis of Mycelium Growth and Mycelium Density In Vitro of Pleurotus ostreatus with Submerged Fermentation as Substrate Treatment. 以深层发酵为底物处理的平菇体外菌丝生长和菌丝密度的数字分析。
IF 1.6 4区 生物学
Mycobiology Pub Date : 2025-02-13 eCollection Date: 2025-01-01 DOI: 10.1080/12298093.2025.2460291
Jorge Andres Magaña Amaya, Naoto Shimizu
{"title":"Digital Analysis of Mycelium Growth and Mycelium Density <i>In Vitro</i> of <i>Pleurotus ostreatus</i> with Submerged Fermentation as Substrate Treatment.","authors":"Jorge Andres Magaña Amaya, Naoto Shimizu","doi":"10.1080/12298093.2025.2460291","DOIUrl":"10.1080/12298093.2025.2460291","url":null,"abstract":"<p><p>Edible mushroom cultivation often involves sterilizing the substrate, or a similar heat process like pasteurization, to facilitate mycelial colonization. Chemical treatments are an alternative approach that is also employed in some regions. Mycelial growth and density were analyzed <i>in vitro</i> by capturing daily photographs using a digital camera, with sterilized substrate serving as the control treatment. Our findings revealed that both fermented and sterilized substrates had similar growth patterns, although fermented treatment required a longer incubation time for full substrate colonization. Mycelium in fermented substrate had a denser structure compared to sterilized treatment, reflecting the interactions with the naturally-present microorganisms within the substrate. Conversely, mycelium in sterilized treatment exhibited faster substrate colonization times but had a less dense mycelial structure. Yeast and bacterial colonies were present throughout the fermentation process and 7 days after <i>P. ostreatus</i> mycelium inoculation, indicating active microbial communities during colonization. An initial decrease in CFU on the 3<sup>rd</sup> day, followed by an increase by the 7<sup>th</sup> day, suggests a shift toward anaerobic and facultative microorganisms due to oxygen depletion during fermentation. Mold colonies disappeared by the end of fermentation. Despite the complex interactions between yeast, bacteria, and <i>P. ostreatus</i> mycelium, the naturally-present microorganisms in the substrate appear to have at least neutral effects, enabling mycelial growth.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"53 2","pages":"214-224"},"PeriodicalIF":1.6,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912283/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Generation of a Clean Host for Polyketide Production Using Agricultural Wastes in Ascochyta Rabiei. 利用农业废弃物生产聚酮清洁寄主的研究
IF 1.6 4区 生物学
Mycobiology Pub Date : 2025-02-13 eCollection Date: 2025-01-01 DOI: 10.1080/12298093.2025.2460292
Hongbee Han, Rundong Liu, Jung-Jae Woo, Jae-Seoun Hur, Wonyong Kim
{"title":"Generation of a Clean Host for Polyketide Production Using Agricultural Wastes in <i>Ascochyta Rabiei</i>.","authors":"Hongbee Han, Rundong Liu, Jung-Jae Woo, Jae-Seoun Hur, Wonyong Kim","doi":"10.1080/12298093.2025.2460292","DOIUrl":"https://doi.org/10.1080/12298093.2025.2460292","url":null,"abstract":"<p><p>The plant pathogenic fungus <i>Ascochyta rabiei</i> produces solanapyrones, which are polyketide-derived secondary metabolites, during its saprobic growth on straws. Previously, we utilized <i>A. rabiei</i> as a heterologous host to produce a lichen-derived polyketide with anticancer activities. This study aimed to establish <i>A. rabiei</i> as a sustainable biorefinery for production of biologically active compounds through two objectives: optimizing culture conditions on agricultural waste substrates for polyketide production and generating a clean host using the Cre-loxP system for reusable antibiotic resistance markers. We found that soy hull is the most effective substrate among lignocellulosic materials. Neither light nor the addition of extra divalent cations were required for solanapyrone production in <i>A. rabiei</i>. Production of solanapyrones peaked 18 days after culture on soy hull pellets. To generate a clean host, we deleted the <i>pks1</i> gene, involved in melanin biosynthesis, and the <i>sol1</i> gene, responsible for solanapyrone production. Antibiotic resistance markers used in genetic transformation were recycled by establishing a Cre-loxP system in <i>A. rabiei</i>. In this system, Cre recombinase was expressed under the control of a promoter inducible during sporulation to mitigate the cytotoxicity of Cre. Here, we set the groundwork for developing <i>A. rabiei</i> as an environmentally-friendly biorefinery by generating a clean host with a Cre-loxP marker recycling system and optimizing growth conditions with soy hull pellets.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"53 2","pages":"225-235"},"PeriodicalIF":1.6,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12010432/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144013735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Trends in Candida albicans Bloodstream Infections and Antifungal Resistance in a Tertiary Care Hospital in South Korea (2013-2023). 韩国三级医院白色念珠菌血液感染和抗真菌耐药性趋势(2013-2023)
IF 1.6 4区 生物学
Mycobiology Pub Date : 2025-02-12 eCollection Date: 2025-01-01 DOI: 10.1080/12298093.2025.2461336
Ki-Yeon Kim, Ga-Yeon Kim, Jae Kyung Kim
{"title":"Trends in <i>Candida albicans</i> Bloodstream Infections and Antifungal Resistance in a Tertiary Care Hospital in South Korea (2013-2023).","authors":"Ki-Yeon Kim, Ga-Yeon Kim, Jae Kyung Kim","doi":"10.1080/12298093.2025.2461336","DOIUrl":"10.1080/12298093.2025.2461336","url":null,"abstract":"<p><p>In this study, we aimed to analyze the annual, age- and sex-specific frequencies and antifungal resistance rates of <i>Candida albicans</i> infections over an 11-year period (2013 - 2023) at a university hospital in South Chungcheong Province, South Korea. A total of 476 cases identified as <i>C. albicans</i> from 260,769 blood cultures were analyzed retrospectively. The annual number of <i>C. albicans</i> isolates increased by 161.1% in 2019 and 136.7% in 2022 compared with that in the previous year. The mean age of patients from whom <i>C. albicans</i> was isolated was 65.8 years, with 51.8% patients being aged 60-79 years. The number of isolates was higher in male patients than in female patients (male-to-female ratio: 1.4:1). Of the isolates, 6.6% were resistant to flucytosine and 4.5% were resistant to voriconazole, whereas all isolates were susceptible to caspofungin and micafungin. Overall, this study provides vital foundational data for tertiary general hospitals in the Chungcheong region and for developing national infection management strategies, thereby providing a valuable basis for future in-depth studies.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"53 2","pages":"236-242"},"PeriodicalIF":1.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Shape-Shifting Mechanisms: Integrative Multi-Omics Insights Into Candida albicans Morphogenesis. 变形机制:对白色念珠菌形态发生的多指标综合洞察。
IF 1.6 4区 生物学
Mycobiology Pub Date : 2025-02-12 eCollection Date: 2025-01-01 DOI: 10.1080/12298093.2025.2460304
Kyunghun Min, Aerin Park
{"title":"Shape-Shifting Mechanisms: Integrative Multi-Omics Insights Into <i>Candida albicans</i> Morphogenesis.","authors":"Kyunghun Min, Aerin Park","doi":"10.1080/12298093.2025.2460304","DOIUrl":"10.1080/12298093.2025.2460304","url":null,"abstract":"<p><p>The ability of <i>Candida albicans</i> to switch among yeast, hyphal, and pseudohyphal forms underlies its adaptability and pathogenicity. While cAMP-dependent signaling has long been considered central to hyphal growth, recent multi-omics studies show that cAMP-independent mechanisms also drive morphological changes. Basal PKA activity, cyclin-dependent kinases (e.g., Cdc28), and other regulators can promote shape-shifting even without classical cAMP pathways. In addition, N-acetylglucosamine (GlcNAc) acts as a potent signal that induces hyphal growth independently of its metabolic role, directly connecting environmental cues to morphological states. By integrating transcriptomic, proteomic, and phosphoproteomic data, this review exposes the intricate networks controlling <i>C. albicans</i> morphogenesis. A clearer understanding of these complex regulatory circuits lays the groundwork for future studies that employ advanced multi-omics analyses. Such approaches will help elucidate how these pathways converge, how they respond to changing environments, and how they might be harnessed or disrupted to influence fungal behavior.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"53 2","pages":"250-257"},"PeriodicalIF":1.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912286/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Growth Characteristics of Pseudomonas putida and Pleurotus ostreatus After Co-Cultivation. 恶臭假单胞菌与平菇共培养后的生长特性。
IF 1.6 4区 生物学
Mycobiology Pub Date : 2025-02-11 eCollection Date: 2025-01-01 DOI: 10.1080/12298093.2024.2436774
Yu-Bin Park, Youn-Jin Park, Myoung-Jun Jang
{"title":"Growth Characteristics of <i>Pseudomonas putida</i> and <i>Pleurotus ostreatus</i> After Co-Cultivation.","authors":"Yu-Bin Park, Youn-Jin Park, Myoung-Jun Jang","doi":"10.1080/12298093.2024.2436774","DOIUrl":"10.1080/12298093.2024.2436774","url":null,"abstract":"<p><p>This study was conducted to investigate the growth characteristics and morphology of <i>Pleurotus ostreatus</i> mycelium during co-cultivation with <i>Pseudomonas putida</i>. For selection of the most effective <i>Pseudomona</i>s species in coculture, Three strains of <i>Pseudomonas putida</i> and one strain <i>of Pseudomonas fluorescens</i> were co-cultured with heuktari, one of the varieties of <i>P. ostreatus</i>, on NPDA agar medium to observe the growth pattern of mycelium. It was found that the microorganism affecting mycelium growth during co-cultivation was <i>P. putida</i> KACC 10275, and co-cultivation of these two species resulted in enhanced mycelium growth on both NPDA and sawdust agar media. Furthermore, while primordium formation and fruit body development did not occur on plates inoculated only with heuktari, fruit bodies were observed only on plates where heuktari and <i>P. putida</i> were co-cultured, as confirmed by cultivation in the growth chamber for the same duration.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"53 1","pages":"72-78"},"PeriodicalIF":1.6,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11841171/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inocybe crenata sp. nov. (subsec. Geophyllinae, sect. Tardae) from Conifer Dominated Forests of Pakistan. 赤藓属11月(亚组)地茶树科,跗科),产自巴基斯坦针叶林。
IF 1.6 4区 生物学
Mycobiology Pub Date : 2025-02-10 eCollection Date: 2025-01-01 DOI: 10.1080/12298093.2024.2398273
Urooj Ashraf, Gordon Webster, Annum Razzaq, Najam-Ul-Sehar Afshan, Sarah R Christofides, Arooj Naseer, Muhammad Ali, Abdul Rehman Niazi, Andrew J Weightman, Abdul Nasir Khalid
{"title":"<i>Inocybe crenata</i> sp. nov. (subsec. <i>Geophyllinae</i>, sect. <i>Tardae</i>) from Conifer Dominated Forests of Pakistan.","authors":"Urooj Ashraf, Gordon Webster, Annum Razzaq, Najam-Ul-Sehar Afshan, Sarah R Christofides, Arooj Naseer, Muhammad Ali, Abdul Rehman Niazi, Andrew J Weightman, Abdul Nasir Khalid","doi":"10.1080/12298093.2024.2398273","DOIUrl":"10.1080/12298093.2024.2398273","url":null,"abstract":"<p><p><i>Inocybe crenata</i> sp. nov. (subsec. <i>Geophyllinae</i>, sect. <i>Tardae</i>) has been described from conifer dominated forests of Pakistan. <i>Inocybe crenata</i> differs from other related species by having a pale-yellow pileus with crenate margins and a bumpy or cracked center, with a fibrillose stipe. Phylogenetic analysis based on nuclear ribosomal large subunit (LSU) and internal transcribed spacer (nrITS) sequence data supported the identity of <i>Inocybe crenate</i> as a distinct taxon. A detailed description of this novel species is provided.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"53 2","pages":"135-143"},"PeriodicalIF":1.6,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912245/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phylogenetic and Taxonomic Study of Asteroconium saccardoi: A Fungal Species with Aster-shaped Spores Infecting Machilus thunbergii. Asteroconium saccardoi 的系统发育和分类研究:一种感染 Machilus thunbergii 的具有菊形孢子的真菌物种。
IF 1.6 4区 生物学
Mycobiology Pub Date : 2025-02-08 eCollection Date: 2025-01-01 DOI: 10.1080/12298093.2025.2457192
Dong Jae Lee, Ji-Hyun Park, Young-Joon Choi
{"title":"Phylogenetic and Taxonomic Study of <i>Asteroconium saccardoi</i>: A Fungal Species with Aster-shaped Spores Infecting <i>Machilus thunbergii</i>.","authors":"Dong Jae Lee, Ji-Hyun Park, Young-Joon Choi","doi":"10.1080/12298093.2025.2457192","DOIUrl":"10.1080/12298093.2025.2457192","url":null,"abstract":"<p><p>Small galls and white blisters with cup-like fungal structures were observed on the leaves of <i>Machilus thunbergii</i> (Lauraceae), a tree species native to East Asia. Based on its distinctive symptoms and aster-shaped spores, the causal agent was identified as <i>Asteroconium saccardoi</i>. Despite its unique characteristics, the genus <i>Asteroconium</i> remains taxonomically unresolved (incertae sedis) within the phylum Ascomycota due to the absence of both sequence data and available culture. The present study successfully obtained ITS and LSU rDNA sequences from its fresh spore masses formed on <i>M. thunbergii</i>. Phylogenetic analysis revealed that <i>A. saccardoi</i> belongs to the order Exobasidiales under Basidiomycota. Detailed morphological characteristics of basidia and basidiospores were documented, providing comprehensive insights into the taxonomy and phylogeny of <i>Asteroconium</i> within Exobasidiales.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"53 1","pages":"128-133"},"PeriodicalIF":1.6,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11841111/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preussia jejuensis sp. nov., P. koreensis sp. nov., and P. isomera, Coprophilous Fungi Isolated from Horse Dung in Seopjikoji, Jeju Island in the Republic of Korea. 济州岛Seopjikoji马粪中分离的粪菌Preussia jejuensis sp. nov.、p.k reensis sp. nov.和p.r isomera .。
IF 1.6 4区 生物学
Mycobiology Pub Date : 2025-02-07 eCollection Date: 2025-01-01 DOI: 10.1080/12298093.2025.2455214
HyeongJin Noh, Hyun Uk Cho, Seong Hwan Kim
{"title":"<i>Preussia jejuensis</i> sp. nov., <i>P. koreensis</i> sp. nov., and <i>P. isomera,</i> Coprophilous Fungi Isolated from Horse Dung in Seopjikoji, Jeju Island in the Republic of Korea.","authors":"HyeongJin Noh, Hyun Uk Cho, Seong Hwan Kim","doi":"10.1080/12298093.2025.2455214","DOIUrl":"10.1080/12298093.2025.2455214","url":null,"abstract":"<p><p>The genus <i>Preussia</i> is widely distributed and includes species with ecological and biotechnological importance. In this study, morphological and phylogenetic analyses of the ITS and LSU rDNA sequences revealed two novel species, <i>P. jejuensis</i> sp. nov. and <i>P. koreensis</i> sp. nov., as well as one previously unrecorded species in Korea, <i>P. isomera</i>, from horse dung collected in Seopjikoji, Jeju Island, Republic of Korea. <i>P. jejuensis</i> sp. nov. is unique in producing conidia from conidiomata instead of teleomorphic structures, a feature not observed in any other known species of the <i>Preussia</i> genus. <i>P. koreensis</i> sp. nov. is morphologically distinguished by the absence of a neck, a smaller ascocarp diameter, smaller asci, and larger ascospores. In the phylogenetic analysis, <i>P. jejuensis</i> sp. nov. was closely related to <i>P. isomera</i> and <i>S. minimoides</i>, while <i>P. koreensis</i> sp. nov. was closely related to <i>P. arizonica</i>, <i>P. persica</i>, and <i>S. minima</i>. However, <i>P. jejuensis</i> sp. nov. and <i>P. koreensis</i> sp. nov. were clearly distinguished from their related species. This study expands the understanding of the biodiversity of coprophilous fungi and its distribution in Jeju Island, a region where horse breeding has been practiced for centuries, emphasizing the ecological importance of dung as a fungal habitat.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"53 2","pages":"200-213"},"PeriodicalIF":1.6,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An Improved Sporulation Method for Producing Asexual Zoospores of Pythium aphanidermatum on Culture Medium. 一种在培养基上产生无性游动孢子的改进产孢方法。
IF 1.6 4区 生物学
Mycobiology Pub Date : 2025-02-04 eCollection Date: 2025-01-01 DOI: 10.1080/12298093.2025.2452640
Yu Jin Kim, Seung Hyo Baek, Elena Volynchikova, Mee Kyung Sang, Ki Deok Kim
{"title":"An Improved Sporulation Method for Producing Asexual Zoospores of <i>Pythium aphanidermatum</i> on Culture Medium.","authors":"Yu Jin Kim, Seung Hyo Baek, Elena Volynchikova, Mee Kyung Sang, Ki Deok Kim","doi":"10.1080/12298093.2025.2452640","DOIUrl":"10.1080/12298093.2025.2452640","url":null,"abstract":"<p><p>An improved sporulation method for <i>Pythium aphanidermatum</i> on 10% V<sub>8</sub>-juice agar was developed to generate substantial quantities of zoospores. We conducted experiments using a 4 × 4 factorial design to evaluate the effects of temperature (20, 25, 30, and 35 °C) and incubation time (2, 3, 4, and 5 days) on zoospore production from isolates HSv05 and KACC 48066. The results indicated that a temperature of 30 °C consistently yielded the highest numbers of zoospores across all incubation periods. Applying the same temperature (30 °C) and all incubation periods to other isolates (CCp03, CCp04, CCp05, HSv10, and KACC 40156) also resulted in higher numbers of zoospores regardless of incubation times compared with that under the control condition (25 °C and 2 days). Further virulence tests of isolate HSv05 revealed that high concentrations of zoospores (5 × 10<sup>2</sup> - 10<sup>5</sup> zoospores/ml) induced severe rot symptoms; however, a low concentration of 10<sup>2</sup> zoospores/ml produced only considerably weak symptoms on inoculated potato tubers. No symptoms appeared in tubers inoculated with 0 (uninoculated control) or 10<sup>1</sup> zoospores/ml. These findings suggest that our sporulation method can help obtain adequate zoospores for various basic and applied studies, including pathogen identification, virulence assessment, resistance resource screening, and control strategy development.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"53 1","pages":"120-127"},"PeriodicalIF":1.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11841104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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