{"title":"以深层发酵为底物处理的平菇体外菌丝生长和菌丝密度的数字分析。","authors":"Jorge Andres Magaña Amaya, Naoto Shimizu","doi":"10.1080/12298093.2025.2460291","DOIUrl":null,"url":null,"abstract":"<p><p>Edible mushroom cultivation often involves sterilizing the substrate, or a similar heat process like pasteurization, to facilitate mycelial colonization. Chemical treatments are an alternative approach that is also employed in some regions. Mycelial growth and density were analyzed <i>in vitro</i> by capturing daily photographs using a digital camera, with sterilized substrate serving as the control treatment. Our findings revealed that both fermented and sterilized substrates had similar growth patterns, although fermented treatment required a longer incubation time for full substrate colonization. Mycelium in fermented substrate had a denser structure compared to sterilized treatment, reflecting the interactions with the naturally-present microorganisms within the substrate. Conversely, mycelium in sterilized treatment exhibited faster substrate colonization times but had a less dense mycelial structure. Yeast and bacterial colonies were present throughout the fermentation process and 7 days after <i>P. ostreatus</i> mycelium inoculation, indicating active microbial communities during colonization. An initial decrease in CFU on the 3<sup>rd</sup> day, followed by an increase by the 7<sup>th</sup> day, suggests a shift toward anaerobic and facultative microorganisms due to oxygen depletion during fermentation. Mold colonies disappeared by the end of fermentation. Despite the complex interactions between yeast, bacteria, and <i>P. ostreatus</i> mycelium, the naturally-present microorganisms in the substrate appear to have at least neutral effects, enabling mycelial growth.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"53 2","pages":"214-224"},"PeriodicalIF":2.0000,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912283/pdf/","citationCount":"0","resultStr":"{\"title\":\"Digital Analysis of Mycelium Growth and Mycelium Density <i>In Vitro</i> of <i>Pleurotus ostreatus</i> with Submerged Fermentation as Substrate Treatment.\",\"authors\":\"Jorge Andres Magaña Amaya, Naoto Shimizu\",\"doi\":\"10.1080/12298093.2025.2460291\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Edible mushroom cultivation often involves sterilizing the substrate, or a similar heat process like pasteurization, to facilitate mycelial colonization. Chemical treatments are an alternative approach that is also employed in some regions. Mycelial growth and density were analyzed <i>in vitro</i> by capturing daily photographs using a digital camera, with sterilized substrate serving as the control treatment. Our findings revealed that both fermented and sterilized substrates had similar growth patterns, although fermented treatment required a longer incubation time for full substrate colonization. Mycelium in fermented substrate had a denser structure compared to sterilized treatment, reflecting the interactions with the naturally-present microorganisms within the substrate. Conversely, mycelium in sterilized treatment exhibited faster substrate colonization times but had a less dense mycelial structure. Yeast and bacterial colonies were present throughout the fermentation process and 7 days after <i>P. ostreatus</i> mycelium inoculation, indicating active microbial communities during colonization. An initial decrease in CFU on the 3<sup>rd</sup> day, followed by an increase by the 7<sup>th</sup> day, suggests a shift toward anaerobic and facultative microorganisms due to oxygen depletion during fermentation. Mold colonies disappeared by the end of fermentation. Despite the complex interactions between yeast, bacteria, and <i>P. ostreatus</i> mycelium, the naturally-present microorganisms in the substrate appear to have at least neutral effects, enabling mycelial growth.</p>\",\"PeriodicalId\":18825,\"journal\":{\"name\":\"Mycobiology\",\"volume\":\"53 2\",\"pages\":\"214-224\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-02-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912283/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mycobiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/12298093.2025.2460291\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycobiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/12298093.2025.2460291","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
Digital Analysis of Mycelium Growth and Mycelium Density In Vitro of Pleurotus ostreatus with Submerged Fermentation as Substrate Treatment.
Edible mushroom cultivation often involves sterilizing the substrate, or a similar heat process like pasteurization, to facilitate mycelial colonization. Chemical treatments are an alternative approach that is also employed in some regions. Mycelial growth and density were analyzed in vitro by capturing daily photographs using a digital camera, with sterilized substrate serving as the control treatment. Our findings revealed that both fermented and sterilized substrates had similar growth patterns, although fermented treatment required a longer incubation time for full substrate colonization. Mycelium in fermented substrate had a denser structure compared to sterilized treatment, reflecting the interactions with the naturally-present microorganisms within the substrate. Conversely, mycelium in sterilized treatment exhibited faster substrate colonization times but had a less dense mycelial structure. Yeast and bacterial colonies were present throughout the fermentation process and 7 days after P. ostreatus mycelium inoculation, indicating active microbial communities during colonization. An initial decrease in CFU on the 3rd day, followed by an increase by the 7th day, suggests a shift toward anaerobic and facultative microorganisms due to oxygen depletion during fermentation. Mold colonies disappeared by the end of fermentation. Despite the complex interactions between yeast, bacteria, and P. ostreatus mycelium, the naturally-present microorganisms in the substrate appear to have at least neutral effects, enabling mycelial growth.
期刊介绍:
Mycobiology is an international journal devoted to the publication of fundamental and applied investigations on all aspects of mycology and their traditional allies. It is published quarterly and is the official publication of the Korean Society of Mycology. Mycobiology publishes reports of basic research on fungi and fungus-like organisms, including yeasts, filamentous fungi, lichen fungi, oomycetes, moulds, and mushroom. Topics also include molecular and cellular biology, biochemistry, metabolism, developmental biology, environmental mycology, evolution, ecology, taxonomy and systematics, genetics/genomics, fungal pathogen and disease control, physiology, and industrial biotechnology using fungi.