以深层发酵为底物处理的平菇体外菌丝生长和菌丝密度的数字分析。

IF 2 4区 生物学 Q2 AGRONOMY
Mycobiology Pub Date : 2025-02-13 eCollection Date: 2025-01-01 DOI:10.1080/12298093.2025.2460291
Jorge Andres Magaña Amaya, Naoto Shimizu
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引用次数: 0

摘要

食用菌的培养通常涉及对基质消毒,或类似的加热过程,如巴氏灭菌,以促进菌丝体定植。化学处理是另一种方法,在一些地区也被采用。通过使用数码相机拍摄每日照片来分析菌丝生长和密度,并用灭菌的底物作为对照处理。我们的研究结果表明,发酵和灭菌的底物具有相似的生长模式,尽管发酵处理需要更长的孵育时间才能完全定殖。与灭菌处理相比,发酵底物中的菌丝体结构更致密,反映了与底物中自然存在的微生物的相互作用。相反,灭菌处理的菌丝体表现出更快的底物定植时间,但菌丝结构密度较低。酵母和细菌菌落存在于整个发酵过程和接种后7天,表明在定植过程中微生物群落活跃。CFU在第3天开始下降,随后在第7天增加,表明由于发酵过程中的氧气消耗,向厌氧和兼性微生物转变。霉菌菌落在发酵结束时消失。尽管酵母、细菌和P. ostreatus菌丝体之间存在复杂的相互作用,但底物中自然存在的微生物似乎至少具有中性作用,使菌丝体能够生长。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Digital Analysis of Mycelium Growth and Mycelium Density <i>In Vitro</i> of <i>Pleurotus ostreatus</i> with Submerged Fermentation as Substrate Treatment.

Digital Analysis of Mycelium Growth and Mycelium Density <i>In Vitro</i> of <i>Pleurotus ostreatus</i> with Submerged Fermentation as Substrate Treatment.

Digital Analysis of Mycelium Growth and Mycelium Density <i>In Vitro</i> of <i>Pleurotus ostreatus</i> with Submerged Fermentation as Substrate Treatment.

Digital Analysis of Mycelium Growth and Mycelium Density In Vitro of Pleurotus ostreatus with Submerged Fermentation as Substrate Treatment.

Edible mushroom cultivation often involves sterilizing the substrate, or a similar heat process like pasteurization, to facilitate mycelial colonization. Chemical treatments are an alternative approach that is also employed in some regions. Mycelial growth and density were analyzed in vitro by capturing daily photographs using a digital camera, with sterilized substrate serving as the control treatment. Our findings revealed that both fermented and sterilized substrates had similar growth patterns, although fermented treatment required a longer incubation time for full substrate colonization. Mycelium in fermented substrate had a denser structure compared to sterilized treatment, reflecting the interactions with the naturally-present microorganisms within the substrate. Conversely, mycelium in sterilized treatment exhibited faster substrate colonization times but had a less dense mycelial structure. Yeast and bacterial colonies were present throughout the fermentation process and 7 days after P. ostreatus mycelium inoculation, indicating active microbial communities during colonization. An initial decrease in CFU on the 3rd day, followed by an increase by the 7th day, suggests a shift toward anaerobic and facultative microorganisms due to oxygen depletion during fermentation. Mold colonies disappeared by the end of fermentation. Despite the complex interactions between yeast, bacteria, and P. ostreatus mycelium, the naturally-present microorganisms in the substrate appear to have at least neutral effects, enabling mycelial growth.

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来源期刊
Mycobiology
Mycobiology AGRONOMYMYCOLOGY-MYCOLOGY
CiteScore
3.90
自引率
5.30%
发文量
41
审稿时长
22 weeks
期刊介绍: Mycobiology is an international journal devoted to the publication of fundamental and applied investigations on all aspects of mycology and their traditional allies. It is published quarterly and is the official publication of the Korean Society of Mycology. Mycobiology publishes reports of basic research on fungi and fungus-like organisms, including yeasts, filamentous fungi, lichen fungi, oomycetes, moulds, and mushroom. Topics also include molecular and cellular biology, biochemistry, metabolism, developmental biology, environmental mycology, evolution, ecology, taxonomy and systematics, genetics/genomics, fungal pathogen and disease control, physiology, and industrial biotechnology using fungi.
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