Mycobiology最新文献

{"title":"A New Genus of <i>Myriangiales</i> Order Isolated from a Toenail.","authors":"Anbazhagan Mageswari, Kye Won Choe, Yoojeong Choi, Mi-Kyung Lee, Daseul Lee, Seung-Beom Hong","doi":"10.1080/12298093.2026.2735733","DOIUrl":"10.1080/12298093.2026.2735733","url":null,"abstract":"<p><p>Clarifying the phylogenetic positions of microorganisms is a primary area of expertise of microbial culture collection centers. In the present study, we aimed to elucidate the phylogenetic position of a fungal strain, previously reported from a human toenail. The earlier report was able to resolve its phylogeny only until genus level (<i>Elsinoë</i>). After being deposited in KACC as strain KACC 410304, a comprehensive examination was conducted which included studying its morphological features as well as sequences of the internal transcribed spacer (ITS), large subunit (LSU), partial translation elongation factor 1-alpha (<i>TEF1-α</i>) and partial DNA-directed RNA polymerase II second LSU (<i>RPB2</i>) to resolve its taxonomic position. Morphological and microscopic observations indicated colonies to be slow-growing, grayish sepia on potato dextrose agar, apricot to brown color on malt extract agar and produce enteroblastic, monophialidic, hyaline, smooth to verruculose, ampulliform to doliiform conidiogenous cells. Conidia were hyaline, granular, aseptate, guttulate, ellipsoid to oblong, apex obtuse, and club shaped. Maximum-likelihood phylogenetic tree based on the combined four nuclear markers (ITS, LSU, <i>TEF1-α</i>, and <i>RPB2</i>) revealed that the strain grouped within the order <i>Myriangiales</i> and formed an independent lineage. Therefore, we propose that the strain does not fall under family <i>Myriangiaceae</i> or <i>Elsinoaceae</i>, but separates into a distinct clade within the order <i>Myriangiales</i>. We describe here <i>Cerebroclavomyces unguium</i> gen nov. et sp. nov. as <i>incertae sedis</i> within <i>Myriangiales</i> using KACC 410304 as its ex-type culture.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 5","pages":"557-568"},"PeriodicalIF":2.5,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13665690/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149195374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taxonomic Reevaluation of <i>Blackwellomyces</i> Isolates Preserved in Korean Agricultural Culture Collection and Description of Two New Species.","authors":"Donghun Kang, Jae Sung Lee, Le Dinh Thao, Daseul Lee, Anbazhagan Mageswari, Jae Su Kim, Tae Young Shin, Seung-Beom Hong","doi":"10.1080/12298093.2026.2726644","DOIUrl":"10.1080/12298093.2026.2726644","url":null,"abstract":"<p><p><i>Blackwellomyces</i> is a genus of entomopathogenic fungi in the family Cordycipitaceae (Hypocreales, Sordariomycetes, Ascomycota) that primarily infects arthropod larvae, particularly those of Lepidoptera. Despite its ecological significance, this genus remains poorly studied in Korea. During the re-identification of Korean <i>Cordyceps</i> sensu lato isolates preserved in the Korean Agricultural Culture Collection (KACC), 12 strains were found to belong to the genus <i>Blackwellomyces</i>. Accordingly, this study aimed to accurately identify and clarify the taxonomic status of these isolates. The 12 <i>Blackwellomyces</i> strains were identified using molecular markers, including the internal transcribed spacer (ITS), large subunit rDNA (LSU), translation elongation factor 1-alpha (<i>TEF</i>), and the largest (<i>RPB1</i>) and second-largest (<i>RPB2</i>) subunits of RNA polymerase II. Phylogenetic analyses based on multilocus sequence data revealed that the isolates formed two distinct clades, which were further supported by morphological observations, representing novel species within the genus. Consequently, two new species, <i>Blackwellomyces koreensis</i> sp. nov. and <i>Blackwellomyces larvicola</i> sp. nov., are described from Korea.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 4","pages":"547-556"},"PeriodicalIF":2.5,"publicationDate":"2026-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13573503/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148970081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Five New Records of Endophytic Fungi Isolated from <i>Sargassum</i> spp. in Korea.","authors":"Yu-Na Choi, Ji-Won Kim, Seong-Eun Kim, Ahn-Heum Eom","doi":"10.1080/12298093.2026.2719984","DOIUrl":"10.1080/12298093.2026.2719984","url":null,"abstract":"<p><p>Five endophytic fungal species were isolated from the brown macroalgae <i>Sargassum muticum</i> and <i>Sargassum thunbergii</i> collected from coastal regions of Korea. The isolates were identified based on morphological characteristics and phylogenetic analyses using combinations of internal transcribed spacer (ITS), large subunit ribosomal RNA (LSU), β-tubulin (TUB), translation elongation factor 1-alpha (TEF), calmodulin (CaM), and RNA polymerase II second largest subunit (RPB2) gene sequences. The identified species were <i>Hypoxylon cinnabarinum</i>, <i>Neopestalotiopsis asiatica</i>, <i>Allophoma hayatii</i>, <i>Sclerostagonospora cycadis</i>, and <i>Aspergillus occultus</i>. Phylogenetic analyses based on multilocus sequence data supported the taxonomic placement of all isolates, and their morphological characteristics were consistent with those of previously described species. This study represents the first report of <i>H. cinnabarinum</i>, <i>N. asiatica</i>, <i>A. hayatii</i>, <i>S. cycadis</i>, and <i>A. occultus</i> in Korea. These findings expand the known diversity of endophytic fungi associated with <i>Sargassum</i> species and provide baseline taxonomic information for future studies of seaweed-associated fungal communities in Korean coastal ecosystems.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 4","pages":"536-546"},"PeriodicalIF":2.5,"publicationDate":"2026-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13573538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148970057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of dsRNA in Soil-Derived Ascomycetes and Characterization of Cladosporium cladosporioides Partitivirus 1 Isolate IPBL11.","authors":"Seojin Moon, Dong Young Hwang, Robert H A Coutts, Ioly Kotta-Loizou, Tae Young Shin","doi":"10.1080/12298093.2026.2719329","DOIUrl":"10.1080/12298093.2026.2719329","url":null,"abstract":"<p><p>Mycoviruses can induce phenotypic and physiological changes in their fungal hosts, making them valuable biological resources. To harness this potential, it is crucial to gather comprehensive information on their distribution patterns, genomic and structural characteristics, and interactions with host fungi. In this study, we screened 64 ascomycete isolates collected from various soil environments in Korea to detect the presence of double-stranded RNA (dsRNA) elements. We identified dsRNA bands in three of these isolates. Among them, we determined the complete genome sequence of a bipartite dsRNA virus found in <i>Cladosporium anthropophilum</i>. Phylogenetic analyses based on the RNA-dependent RNA polymerase (RdRP) and capsid protein (CP) sequences indicated that this virus belongs to the genus <i>Gammapartitivirus</i> within the family <i>Partitiviridae</i>. Comparative sequence analyses suggested that this virus is best classified as a new isolate of Cladosporium cladosporioides partitivirus 1, which we designated as Cladosporium cladosporioides partitivirus 1 isolate IPBL11 (CcPV1-IPBL11).</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 4","pages":"527-535"},"PeriodicalIF":2.5,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13560416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148945028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variation in Culturable Rhizosphere Fungal Communities of <i>Abies koreana</i> in Mt. Jiri, Korea, in Relation to Topographic and Physicochemical Soil Properties.","authors":"Jong Myong Park, Minjeong Kim, Chang-Gi Back, Ji Won Hong, Jin-Soo Son, Ki-Eun Lee, Jin-Won Kim, Hyeon-Ho Myeong, Young-Hyun You","doi":"10.1080/12298093.2026.2706307","DOIUrl":"10.1080/12298093.2026.2706307","url":null,"abstract":"<p><p>This study was aimed at characterizing the culturable rhizosphere fungal community of <i>Abies koreana</i> on Mt. Jiri, Korea and to investigate the influence of topographic and physicochemical soil properties on them. Samples were collected from two contrasting sites: the Seseok Plateau (gentle-slope basin with poor drainage) and Oesamsinbong (steep slope with excellent drainage). A total of 620 isolates spanning four phyla, 11 classes, 21 orders, 34 families, and 51 genera were identified. <i>Penicillium</i> was the dominant genus, comprising 367 isolates. Shannon's diversity index increased as <i>Penicillium</i> dominance decreased, indicating that reduced exclusive dominance enhanced community evenness. Although both sites had loamy soils, their chemical properties varied significantly with topography. The Seseok Plateau showed extreme acidification (pH 3.80-4.20) and severe cation leaching with a very low average base saturation (BS) of 4.9%. In contrast, Oesamsinbong maintained higher BS (avg. 12.3%) because the steep terrain inhibited acid accumulation. <i>Penicillium</i> dominance was significantly higher in Oesamsinbong (72.7-76.0%) than in the Seseok Plateau (31.9-48.9%). Pearson's correlation revealed a strong positive relationship between the BS and <i>Penicillium</i> dominance (<i>r</i> = 0.957, <i>p</i> = 0.0028). Although available phosphate was higher in the Seseok Plateau, the high <i>Penicillium</i> dominance in Oesamsinbong supports a potential hypothesis regarding microbial contribution to phosphorus availability for the host. Overall, the reduced vigor of <i>A. koreana</i> in the Seseok Plateau was likely associated with impaired microbe-mediated nutrient uptake under extreme acidic stress. Enhancing the rhizosphere fungal ecosystem is crucial for ensuring the sustainability of endangered subalpine conifer forests.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 4","pages":"493-504"},"PeriodicalIF":2.5,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13435269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148674064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and Multilocus Phylogenetic Characterization of <i>Septoria</i> Species from <i>Dioscorea</i>, <i>Eclipta</i>, and <i>Taraxacum</i> in East Asia.","authors":"Joon Ho Choi, Bora Nam, Anysia Hedy Ujat, Chiharu Nakashima, Seong-Jin Lee, Young-Joon Choi, Hyeon-Dong Shin","doi":"10.1080/12298093.2026.2705054","DOIUrl":"10.1080/12298093.2026.2705054","url":null,"abstract":"<p><p>More than 90 species of <i>Septoria</i> have been reported from Korea, but the identity of many recorded taxa remains uncertain owing to the lack of detailed morphological documentation and multilocus sequence data. In this study, isolates collected from symptomatic leaves of economically and medicinally important plants between 2006 and 2024 were examined using morphological observations and multilocus phylogenetic analyses based on six loci (internal transcribed spacer (ITS) region, actin (ACT), β-tubulin (Btub), calmodulin (CAL), elongation factor 1-alpha (EF1-α), and RNA polymerase II second largest subunit (RPB2)). The concatenated dataset resolved three well-supported lineages corresponding to <i>Septoria dioscoreicola</i> on <i>Dioscorea polystachya</i>, <i>S. eclipticola</i> on <i>Eclipta prostrata</i>, and <i>S. miurae</i> on <i>Taraxacum officinale</i>, of which <i>S. dioscoreicola</i> and <i>S. eclipticola</i> are newly recorded in Korea, whereas Korean materials previously reported as <i>S. taraxaci</i> on <i>Taraxacum</i> are re-identified here as <i>S. miurae</i>. Multilocus analyses further demonstrated that <i>S. taraxaci</i> and <i>S. miurae</i> represent two distinct species associated with <i>Taraxacum</i>, with Korean and Japanese isolates corresponding to <i>S. miurae</i>, whereas the third <i>Taraxacum</i>-associated taxon, <i>S. taraxacicola</i>, is regarded as a synonym of <i>S. miurae</i>. Given the economic and medicinal importance of these hosts involved, the occurrence of Septoria leaf spot on these species may represent a potential concern for their cultivation in East Asia. These results provide an updated taxonomic basis for <i>Septoria</i> species occurring in Korea and clarify species boundaries within the <i>Taraxacum</i>-associated complex.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 4","pages":"481-492"},"PeriodicalIF":2.5,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13435289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148673995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revisiting Species Boundaries in <i>Salispina</i>: Morphological Plasticity in Mangrove-Associated Oomycetes, <i>S. lobata</i> and <i>S. hoi</i>.","authors":"Joshua Allen T Saliba, Reuel M Bennett","doi":"10.1080/12298093.2026.2697104","DOIUrl":"10.1080/12298093.2026.2697104","url":null,"abstract":"<p><p><i>Salispina</i> (<i>Salispinaceae</i>, <i>Rhipidiales</i>), a mangrove-associated oomycete, is characterized by uniquely forming aculeolate sporangia. In this study, six <i>Salispina</i> strains were isolated from fallen mangrove leaves collected in coastal sites in the Philippines. A combined strategy of morphology, morphometric analyses, physiology, and multilocus phylogeny assigned the isolates as <i>Salispina lobata</i> (USTCMS 1612, USTCMS 1613, USTCMS 1617) and <i>Salispina hoi</i> (USTCMS 1614, USTCMS 1615, USTCMS 1616). Multilocus phylogenetic analyses consistently assigned the isolates into two strongly supported clades corresponding to the two species. Despite the clear phylogenetic separation, both species exhibited extensive overlaps in sporangial morphology (i.e., shape and spine distribution) across culture conditions. Morphometric principal component analyses revealed broadly overlapping morphospaces, with culture conditions exhibiting a stronger influence on sporangial size than species identity. Agar-grown cultures frequently developed enlarged, lobate to irregular, and extensively aculeolate sporangia, whereas globose to obpyriform forms with apical spines predominated in water cultures. Temperature and salinity growth profiles were broadly overlapping between the two species, showing optimal growth at 30 <math><mo>°</mo> <mi>C</mi></math> and tolerance to moderate salinity levels. These findings demonstrate culture-dependent phenotypic plasticity in <i>Salispina</i> and highlight the importance of integrative taxonomy for delimiting closely related oomycete species with overlapping morphological traits.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 4","pages":"505-526"},"PeriodicalIF":2.5,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13435278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148673983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Species of <i>Sarcoscypha</i>, <i>S. orientalis</i> sp. nov., and a Newly Recorded Species, <i>S. vassiljevae</i>, from Korea.","authors":"Dae Ho Kim, Young-Nam Kwag, Hyung So Kim, Chang Sun Kim, Sang-Kuk Han","doi":"10.1080/12298093.2026.2699543","DOIUrl":"10.1080/12298093.2026.2699543","url":null,"abstract":"<p><p>Two species of <i>Sarcoscypha</i>, including the new species <i>S. orientalis</i> sp. nov. and the first Korean record of <i>S. vassiljevae</i>, are reported based on morphological observations and phylogenetic analyses of internal transcribed spacer (ITS), large subunit (LSU), and RNA polymerase II second largest subunit (RPB2) sequence data. Specimens of <i>S. orientalis</i> sp. nov. are morphologically similar to <i>S. occidentalis</i> but differ in possessing completely sessile apothecia. Phylogenetic analyses revealed that sequences previously attributed to <i>S. occidentalis</i> were separated into two geographically distinct clades corresponding to USA and East Asian lineages; the Korean specimens were placed within the East Asian lineage. Based on this morphological and phylogenetic evidence, the East Asian specimens are proposed as the new species, <i>Sarcoscypha orientalis</i> sp. nov. The remaining specimens were identified as <i>S. vassiljevae</i>, which is characterized by ivory to pale cream hymenial surfaces and ellipsoid ascospores containing a single large guttule. These specimens clustered with reference sequences of <i>S. vassiljevae</i> in the phylogenetic analyses. These findings expand the known diversity of the genus <i>Sarcoscypha</i> in Korea and provide new insights into the geographic differentiation and taxonomic diversity within the genus.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 4","pages":"1-10"},"PeriodicalIF":2.5,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13425526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148653860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viable Spores Can be Obtained and Germinated from Commercially Obtained Dried Morel (<i>Morchella</i>) Mushrooms.","authors":"Anton Kubala, Ivy J Mutai, Samantha Mclean, Catherine E D Rees","doi":"10.1080/12298093.2026.2701586","DOIUrl":"10.1080/12298093.2026.2701586","url":null,"abstract":"<p><p>Morel mushrooms (<i>Morchella</i> sp.) are highly prized edible mushrooms that are still difficult to cultivate despite great interest and advancements made in recent years. Obtaining genetic material of morel mushrooms is necessary to carry out research but may be challenging depending on the region, time of the season, budget, or local legislation. This study assessed whether ascospores could be recovered and germinated from retail dried morel products and whether resulting isolates could be taxonomically identified. Four packets of dried morels from different suppliers were rehydrated, homogenized, and processed to recover spore suspensions. Hemocytometer counts yielded mean spore titers of 1.50 × 10⁵ (A), 4.24 × 10⁶ (B), 5.86 × 10⁶ (C), and 3.51 × 10⁵ (D) spores mL^-1. Aliquots were plated on Sabouraud dextrose agar (SDA) with chloramphenicol and incubated at 24 °C; colony counts indicated cultivable fractions corresponding to c. 2.2% germination for sample A and ∼10-20% for samples B-D. Representative colonies were subcultured, DNA was extracted, and ITS barcodes were generated by Sanger sequencing. Isolates from samples A, B, and D matched <i>Morchella sextelata</i>, while isolates from sample C matched <i>Morchella eximia</i>. The findings demonstrate that viable <i>Morchella</i> ascospores can persist through commercial drying and that dried products constitute a low‑cost source of cultivable material. Discrepancies between sequencing results and product labels highlighted possible country of origin concerns but were considered outside the scope of this work and were not exhaustively investigated.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 4","pages":"464-471"},"PeriodicalIF":2.5,"publicationDate":"2026-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13403543/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148605399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Species and a New Record of the Genus <i>Hydropunctaria</i> from South Korea, with an Updated Key to All Known Species.","authors":"Jizhen Jin, Santosh Joshi, Jae-Seoun Hur","doi":"10.1080/12298093.2026.2697398","DOIUrl":"10.1080/12298093.2026.2697398","url":null,"abstract":"<p><p><i>Hydropunctaria</i> (Verrucariaceae, Ascomycota) is currently circumscribed by 11 species, of which four are known from Asia. The taxonomy and species limits of this genus remain insufficiently understood, particularly in East Asia. Phylogenetic study based on three loci (ITS, mtSSU, and LSU) from populations collected at several localities in South Korea revealed the presence of a hitherto unrecognized species, here described as new species, <i>Hydropunctaria fluminicola</i> sp. nov., collected from fresh water regimes near the littoral zone. The species is characterized by well-developed, gray-green, subgelatinous thallus having distinct punctae and black ridges; algal cells are arranged in columns and perithecia conspicuously protrude above the thallus surface. In addition, a new country record of <i>Hydropunctaria nipponoamphibia</i> is reported. A taxonomic key to all known species of <i>Hydropunctaria</i> is also provided. The present study highlights the taxonomic importance of combining morphological and molecular evidence in resolving species boundaries within <i>Hydropunctaria</i> and contributes to a better understanding of lichen diversity in coastal regions of East Asia.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 4","pages":"452-462"},"PeriodicalIF":2.5,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13390162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}