Mycobiology最新文献_第10页

Metabolite Chemical Composition of the Bletilla striata (Thunb.) Reichb. f. Endophyte Penicillium oxalicum. 白芨(Bletilla striata)的代谢物化学成分Reichb。f.内生真菌:草青霉。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2216944

Ran Liu, Xuehua Han, Jing Gao, Min Luo, Dale Guo, Guangzhi Wang

{"title":"Metabolite Chemical Composition of the Bletilla striata (Thunb.) Reichb. f. Endophyte Penicillium oxalicum.","authors":"Ran Liu, Xuehua Han, Jing Gao, Min Luo, Dale Guo, Guangzhi Wang","doi":"10.1080/12298093.2023.2216944","DOIUrl":"https://doi.org/10.1080/12298093.2023.2216944","url":null,"abstract":"Penicillium oxalicum strain can be isolated from the Bletilla striata (Thunb.) Reichb. f. tubers. Its solid-state fermentation products are concentrated by percolation extraction. Separation and purification have been conducted to the ethyl acetate extracts by preparative HPLC. Based on the use of spectrometry, we have determined 17 known compounds, 12,13-dihydroxy-fumitremorgin C (1), pseurotin A (2), tyrosol (3), cyclo-(L-Pro-L-Val) (4), cis-4-hydroxy-8-O-methylmellein (5), uracil (6), cyclo-(L-Pro-L-Ala) (7), 1,2,3,4-tetrahydro-4-hydroxy-4-quinolin carboxylic acid (8), cyclo-(Gly-L-Pro) (9), 2'-deoxyuridine (10), 1-(β-D-ribofuranosyl)thymine (11), cyclo-(L-Val-Gly) (12), 2'-deoxythymidine (13), cyclo-(Gly-D-Phe) (14), cyclo-L-(4-hydroxyprolinyl)-D-leucine (15), cyclo-(L)-4-hydroxy-Pro-(L)-Phe (16), uridine (17). Here, we report compounds 1-3, 5, 7-8, 11-12, 14-17 are first found and isolated from this endophyte.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"51 3","pages":"148-156"},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d6/0b/TMYB_51_2216944.PMC10288904.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9714404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination and Analysis of Hyper-Variable A Mating Types in Wild Strains of Lentinula edodes in Korea. 韩国香菇野生菌株超变量A交配型的测定与分析。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2022.2161157

Mi-Jeong Park, Eunjin Kim, Yeun Sug Jeong, Mi-Young Son, Yeongseon Jang, Kang-Hyeon Ka

{"title":"Determination and Analysis of Hyper-Variable A Mating Types in Wild Strains of Lentinula edodes in Korea.","authors":"Mi-Jeong Park, Eunjin Kim, Yeun Sug Jeong, Mi-Young Son, Yeongseon Jang, Kang-Hyeon Ka","doi":"10.1080/12298093.2022.2161157","DOIUrl":"https://doi.org/10.1080/12298093.2022.2161157","url":null,"abstract":"The diversity of A mating type in wild strains of Lentinula edodes was extensively analyzed to characterize and utilize them for developing new cultivars. One hundred twenty-three A mating type alleles, including 67 newly discovered alleles, were identified from 106 wild strains collected for the past four decades in Korea. Based on previous studies and current findings, a total of 130 A mating type alleles have been found, 124 of which were discovered from wild strains, indicating the hyper-variability of A mating type alleles of L. edodes. About half of the A mating type alleles in wild strains were found in more than two strains, whereas the other half of the alleles were found in only one strain. About 90% of A mating type combinations in dikaryotic wild strains showed a single occurrence. Geographically, diverse A mating type alleles were intensively located in the central region of the Korean peninsula, whereas only allele A17 was observed throughout Korea. We also found the conservation of the TCCCAC motif in addition to the previously reported motifs, including ATTGT, ACAAT, and GCGGAG, in the intergenic regions of A mating loci. Sequence comparison among some alleles indicated that accumulated mutation and recombination would contribute to the diversification of A mating type alleles in L. edodes. Our data support the rapid evolution of A mating locus in L. edodes, and would help to understand the characteristics of A mating loci of wild strains in Korea and help to utilize them for developing new cultivars.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"51 1","pages":"26-35"},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/57/68/TMYB_51_2161157.PMC9946336.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9342401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro α-Amylase, α-Glucosidase, Pancreatic Lipase, Xanthine Oxidase Inhibiting Activity of Agaricus bisporus Extracts. 双孢蘑菇提取物体外抑制α-淀粉酶、α-葡萄糖苷酶、胰脂肪酶、黄嘌呤氧化酶活性的研究

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2176020

Jung Han Kim, Myoung Jun Jang, Youn Jin Park

{"title":"In Vitro α-Amylase, α-Glucosidase, Pancreatic Lipase, Xanthine Oxidase Inhibiting Activity of Agaricus bisporus Extracts.","authors":"Jung Han Kim, Myoung Jun Jang, Youn Jin Park","doi":"10.1080/12298093.2023.2176020","DOIUrl":"https://doi.org/10.1080/12298093.2023.2176020","url":null,"abstract":"In this study, the α-amylase inhibitory activity, α-glucosidase inhibitory activity, pancreatic lipase inhibitory activity, and Xanthine Oxidase inhibitory activity of the fruiting body extracts of 5 varieties of Agaricus bisporus (AB) were confirmed. First, the α-amylase inhibitory activity of AB12, AB13, AB18, AB34, and AB40 methanol extracts was lower than that of acarbose, a positive control, in all concentration ranges. The α-glucosidase inhibitory activity of the AB40, AB13, and AB12 methanol extracts at the extract concentration of 1.0 mg/mL was 80.5%, 81.3%, and 78.5%, respectively, similar to that of acarbose, a positive control. The pancreatic lipase inhibitory activity of the methanol extract of Agaricus bisporus fruiting body was significantly lower than that of the positive control orlistat in the concentration range of 50 ∼ 1.000 (mg/mL). The Xanthine Oxidase inhibitory activity was 0.5 ∼ 8.0 mg/mL of each extract, which was significantly lower than that of the positive control allopurinol in the same concentration range. However, the Xanthine Oxidase inhibitory activity of AB13 and AB40 at 8.0 mg/mL was about 70%, which was higher than that of other mushrooms. In conclusion, five kinds of Agaricus bisporus fruiting bodies seem to have inhibitory effects on enzymes such as α-amylase, α-glucosidase, pancreatic lipase, and Xanthine Oxidase that degrade starch and protein. In particular, it has an inhibitory effect and a reduction effect on xanthine oxidase that causes gout, so it is expected that it can be developed and used as a food or health supplement with health functional properties through future research.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"51 1","pages":"60-66"},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e0/93/TMYB_51_2176020.PMC9946297.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9358051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome Analysis of Antrodia cinnamomea Mycelia from Different Wood Substrates. 不同木质基质樟芝菌丝体转录组分析。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2175434

Jiao-Jiao Chen, Zhang Zhang, Yi Wang, Xiao-Long Yuan, Juan Wang, Yu-Ming Yang, Yuan Zheng

{"title":"Transcriptome Analysis of Antrodia cinnamomea Mycelia from Different Wood Substrates.","authors":"Jiao-Jiao Chen, Zhang Zhang, Yi Wang, Xiao-Long Yuan, Juan Wang, Yu-Ming Yang, Yuan Zheng","doi":"10.1080/12298093.2023.2175434","DOIUrl":"https://doi.org/10.1080/12298093.2023.2175434","url":null,"abstract":"Antrodia cinnamomea, an edible and medicinal fungus with significant economic value and application prospects, is rich in terpenoids, benzenoids, lignans, polysaccharides, and benzoquinone, succinic and maleic derivatives. In this study, the transcriptome of A. cinnamomea cultured on the wood substrates of Cinnamomum glanduliferum (YZM), C. camphora (XZM), and C. kanehirae (NZM) was sequenced using the high-throughput sequencing technology Illumina HiSeq 2000, and the data were assembled by de novo strategy to obtain 78,729 Unigenes with an N50 of 4,463 bp. Compared with public databases, about 11,435, 6,947, and 5,994 Unigenes were annotated to the Non-Redundant (NR), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genome (KEGG), respectively. The comprehensive analysis of the mycelium terpene biosynthesis-related genes in A. cinnamomea revealed that the expression of acetyl-CoA acetyltransferase (AACT), acyl-CoA dehydrogenase (MCAD), 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA), mevalonate pyrophosphate decarboxylase (MVD), and isopentenyl diphosphate isomerase (IDI) was significantly higher on NZM compared to the other two wood substrates. Similarly, the expression of geranylgeranyltransferase (GGT) was significantly higher on YZM compared to NZM and XZM, and the expression of farnesyl transferase (FTase) was significantly higher on XZM. Furthermore, the expressions of 2,3-oxidized squalene cyclase (OCS), squalene synthase (SQS), and squalene epoxidase (SE) were significantly higher on NZM. Overall, this study provides a potential approach to explore the molecular regulation mechanism of terpenoid biosynthesis in A. cinnamomea.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"51 1","pages":"49-59"},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ef/4f/TMYB_51_2175434.PMC9946338.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9358048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Are Current Aspergillus sojae Strains Originated from a Native Aflatoxigenic Aspergillus Species Population Also Present in California? 目前的大豆曲霉菌株是否起源于当地的黄曲霉种群?

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2217495

Perng-Kuang Chang, Sui Sheng T Hua

{"title":"Are Current Aspergillus sojae Strains Originated from a Native Aflatoxigenic Aspergillus Species Population Also Present in California?","authors":"Perng-Kuang Chang, Sui Sheng T Hua","doi":"10.1080/12298093.2023.2217495","DOIUrl":"https://doi.org/10.1080/12298093.2023.2217495","url":null,"abstract":"Aspergillus sojae has long been considered a domesticated strain of Aspergillus parasiticus. This study delineated relationships among the two species and an Aspergillus PWE36 isolate. Of 25 examined clustered aflatoxin genes of PWE36, 20 gene sequences were identical to those of A. sojae, but all had variations to those of A. parasiticus. Additionally, PWE36 developmental genes of conidiation and sclerotial formation, overall, shared higher degrees of nucleotide sequence identity with A. sojae genes than with A. parasiticus genes. Examination of defective cyclopiazonic acid gene clusters revealed that the PWE36 deletion pattern was identical only to those of A. sojae. Using A. sojae SMF134 genome sequence as a reference, visualization of locally collinear blocks indicated that PWE36 shared higher genome sequence homologies with A. sojae than with A. parasiticus. Phylogenetic inference based on genome-wide single nucleotide polymorphisms (SNPs) and total SNP counts showed that A. sojae strains formed a monophyletic clade and were clonal. Two (Argentinian and Ugandan) A. parasiticus isolates but not including an Ethiopian isolate formed a monophyletic clade, which showed that A. parasiticus population is genetically diverse and distant to A. sojae. PWE36 and A. sojae shared a most recent common ancestor (MRCA). The estimated divergence time for PWE36 and A. sojae was about 0.4 mya. Unlike Aspergillus oryzae, another koji mold that includes genetically diverse populations, the findings that current A. sojae strains formed a monophyletic group and shared the MRCA with PWE36 allow A. sojae to be continuously treated as a species for food safety reasons.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"51 3","pages":"139-147"},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10288891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9706143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Asymbiotic Spore Production of Rhizoglomus intraradices in a Medium Containing Myristate. 在含有肉豆肉酸盐的培养基中，根状芽孢杆菌的非共生孢子产生。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2216500

Jae-Eui Cha, Ahn-Heum Eom

引用次数: 0

A Novel Acremonium Species Isolated from Air Samples in Korea. 从韩国空气样本中分离的一种新的顶孢菌。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2242646

Jung-Min Lee, Jae-Eui Cha, Young-Sil Yoon, Ahn-Heum Eom

引用次数: 0

Isolation and Characterization of Xylanase from a Novel Strain, Penicillium menonorum SP10. menonorum青霉SP10木聚糖酶的分离与鉴定。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2247221

Thi Thu Huong Luong, Supattra Poeaim, Narumon Tangthirasunun

{"title":"Isolation and Characterization of Xylanase from a Novel Strain, Penicillium menonorum SP10.","authors":"Thi Thu Huong Luong, Supattra Poeaim, Narumon Tangthirasunun","doi":"10.1080/12298093.2023.2247221","DOIUrl":"https://doi.org/10.1080/12298093.2023.2247221","url":null,"abstract":"Xylanase has been applied in various sectors, such as biomass conversion, paper, pulp, textiles, and pharmaceutical industries. This study aimed to isolate and screen potential xylanase-producing fungi from the soil of Suphan Buri Province, Thailand. Fifteen fungi were isolated, and their xylanase activities were tested by the qualitative method. The result showed that isolate SP3, SP10 and SP15 gave high xylanase activity with potency index (PI) of 2.32, 2.01 and 1.82, respectively. These fungi were selected for the xylanase quantitative test, isolate SP10 performed the highest xylanase activity with 0.535 U/mL. Through molecular methods using the β-tubulin gene, isolate SP10 was identified as Penicillium menonorum. The xylanase characteristics from P. menonorum SP10 were determined, including the xylanase isoforms and the optimum pH and temperature. The xylanase isoforms on SDS-PAGE indicated that P. menonorum SP10 produced two xylanases (45 and 54 kDa). Moreover, its xylanase worked optimally at pH 6 and 55 °C while reaching 61% activity at 65 °C. These results proposed P. menonorum SP10 as a good candidate for industrial uses, especially in poultry feed and pulp industries, to improve yield and economic efficiency under slightly acidic and high-temperature conditions.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"51 4","pages":"239-245"},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/73/64/TMYB_51_2247221.PMC10498789.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10261165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Identification of Fusarium Basal Rot Pathogens of Onion and Evaluation of Fungicides against the Pathogens. 洋葱基底腐病病原菌鉴定及杀菌剂评价。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2243759

Jong-Hwan Shin, Ha-Kyoung Lee, Chang-Gi Back, Soo-Hyun Kang, Ji-Won Han, Seong-Chan Lee, You-Kyoung Han

{"title":"Identification of Fusarium Basal Rot Pathogens of Onion and Evaluation of Fungicides against the Pathogens.","authors":"Jong-Hwan Shin, Ha-Kyoung Lee, Chang-Gi Back, Soo-Hyun Kang, Ji-Won Han, Seong-Chan Lee, You-Kyoung Han","doi":"10.1080/12298093.2023.2243759","DOIUrl":"https://doi.org/10.1080/12298093.2023.2243759","url":null,"abstract":"Onion (Allium cepa L.) is an economically important vegetable crop worldwide. However, various fungal diseases, including Fusarium basal rot (FBR), neck rot, and white rot, reduce onion production or bulb storage life. FBR caused by Fusarium species is among the most destructive onion diseases. In this study, we identified Fusarium species associated with FBR in Jeolla and Gyeongsang Provinces in South Korea and evaluated fungicides against the pathogens. Our morphological and molecular analyses showed that FBR in onions is associated with Fusarium commune, Fusarium oxysporum, and Fusarium proliferatum. We selected seven fungicides (fludioxonil, hexaconazole, mandestrobin, penthiopyrad, prochloraz-manganese, pydiflumetofen, and tebuconazole) and evaluated their inhibitory effects on mycelial growth of the pathogens at three different concentrations (0.01, 0.1, and 1 mg/mL). We found that prochloraz-manganese was highly effective, inhibiting 100% of the mycelial growth of the pathogens at all concentrations, followed by tebuconazole. Fludioxonil showed < 50% inhibition at 1 mg/mL for the tested isolates.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"51 4","pages":"264-272"},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/59/87/TMYB_51_2243759.PMC10498799.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10268141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Wound Healing Potential of Lignosus rhinocerus and Other Ethno-myco Wound Healing Agents. 犀牛木与其他民族麦可伤口愈合剂的伤口愈合潜力。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2022.2164641

Hui-Yeng Y Yap, Mohammad Farhan Ariffeen Rosli, Soon-Hao Tan, Boon-Hong Kong, Shin-Yee Fung

{"title":"The Wound Healing Potential of Lignosus rhinocerus and Other Ethno-myco Wound Healing Agents.","authors":"Hui-Yeng Y Yap, Mohammad Farhan Ariffeen Rosli, Soon-Hao Tan, Boon-Hong Kong, Shin-Yee Fung","doi":"10.1080/12298093.2022.2164641","DOIUrl":"https://doi.org/10.1080/12298093.2022.2164641","url":null,"abstract":"Wound care has become increasingly important over the years. Various synthetic products for wound care treatment have been reported to cause toxic side effects and therefore natural products are in significant demand as they have minimal side effects. The presence of bioactive compounds in medicinal mushrooms contributes to various biological activities which assist in the early inflammatory phase, keratinocyte proliferation, and its migration enhancement which are pertinent to wound rehabilitation. Lignosus rhinocerus (tiger milk mushroom) can reduce the inflammation phase in wound healing by fighting off bacterial infection and modulating pro-inflammatory cytokines expression in the early stage to avoid prolonged inflammation and tissue damage. The antibacterial, immunomodulating, and anti-inflammatory activities exhibited by most macrofungi play a key role in enhancing wound healing. Several antibacterial and antifungal compounds sourced from traditional botanicals/products may prevent further complications and reoccurrence of injury to a wounded site. Scientific studies are actively underway to ascertain the potential use of macrofungi as a wound healing agent.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"51 1","pages":"1-15"},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/df/54/TMYB_51_2164641.PMC9946334.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9342402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2