Mycobiology最新文献_第10页

Isolation and Cytotoxic Potency of Endophytic Fungi Associated with Dysosma difformis, a Study for the Novel Resources of Podophyllotoxin. 异虫病内生真菌的分离及细胞毒力——鬼臼毒素新资源的研究。

IF 1.9 4区生物学

Mycobiology Pub Date : 2022-09-29 eCollection Date: 2022-01-01 DOI: 10.1080/12298093.2022.2126166

Hoa Thi Tran, Giang Thu Nguyen, Hong Ha Thi Nguyen, Huyen Thi Tran, Quang Hong Tran, Quang Ho Tran, Ngoc Thi Ninh, Phat Tien Do, Ha Hoang Chu, Ngoc Bich Pham

{"title":"Isolation and Cytotoxic Potency of Endophytic Fungi Associated with Dysosma difformis, a Study for the Novel Resources of Podophyllotoxin.","authors":"Hoa Thi Tran, Giang Thu Nguyen, Hong Ha Thi Nguyen, Huyen Thi Tran, Quang Hong Tran, Quang Ho Tran, Ngoc Thi Ninh, Phat Tien Do, Ha Hoang Chu, Ngoc Bich Pham","doi":"10.1080/12298093.2022.2126166","DOIUrl":"https://doi.org/10.1080/12298093.2022.2126166","url":null,"abstract":"Endophytic fungi are promising sources for the production of podophyllotoxin-an important anticancer compound, replacing depleted medical plants. In this study, the endophytes associated with Dysosma difformis-an ethnomedicinal plant species were isolated to explore novel sources of podophyllotoxin. Fifty-three endophytic fungi were isolated and identified by morphological observation and ITS-based rDNA sequencing, assigning them to 27 genera in 3 divisions. Fusarium was found the most prevalent genus with a colonization frequency of 11.11%, followed by Trametes (9.26%) and Penicillium (7.41%). Phylogenetic trees were constructed for the endophytic fungi community in two collection sites, Ha Giang and Lai Chau, revealing the adaptation of the species to the specific tissues and habitats. Cytotoxic activity of endophytic fungal extracts was investigated on cancer cell lines such as SK-LU-1, HL-60, and HepG2, demonstrating strong anti-cancer activity of six isolates belonging to Penicillium, Trametes, Purpureocillium, Aspergillus, and Ganoderma with IC50 value of lower than 10 µg/mL. The presence of podophyllotoxin was indicated in Penicillium, Trametes, Aspergillus and for the first time in Purpureocillium and Ganoderma via high-performance liquid chromatography, which implied them as a potential source of this anti-cancer compound.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/51/19/TMYB_50_2126166.PMC9645267.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40714762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Nuclear Effectors in Plant Pathogenic Fungi. 植物病原真菌的核效应器

IF 1.6 4区生物学

Mycobiology Pub Date : 2022-09-20 eCollection Date: 2022-01-01 DOI: 10.1080/12298093.2022.2118928

Surajit De Mandal, Junhyun Jeon

引用次数: 0

Gene Expression Analyses of Mutant Flammulina velutipes (Enokitake Mushroom) with Clogging Phenomenon. 具有堵塞现象的金针菇突变体基因表达分析。

IF 1.9 4区生物学

Mycobiology Pub Date : 2022-09-19 eCollection Date: 2022-01-01 DOI: 10.1080/12298093.2022.2121497

Ju-Ri Woo, Doo-Ho Choi, Muhammed Taofiq Hamza, Kyung-Oh Doh, Chang-Yoon Lee, Yeon-Sik Choo, Sangman Lee, Jong-Guk Kim, Heeyoun Bunch, Young-Bae Seu

{"title":"Gene Expression Analyses of Mutant Flammulina velutipes (Enokitake Mushroom) with Clogging Phenomenon.","authors":"Ju-Ri Woo, Doo-Ho Choi, Muhammed Taofiq Hamza, Kyung-Oh Doh, Chang-Yoon Lee, Yeon-Sik Choo, Sangman Lee, Jong-Guk Kim, Heeyoun Bunch, Young-Bae Seu","doi":"10.1080/12298093.2022.2121497","DOIUrl":"https://doi.org/10.1080/12298093.2022.2121497","url":null,"abstract":"Regulation of proper gene expression is important for cellular and organismal survival, maintenance, and growth. Abnormal gene expression, even for a single critical gene, can thwart cellular integrity and normal physiology to cause diseases, aging, and death. Therefore, gene expression profiling serves as a powerful tool to understand the pathology of diseases and to cure them. In this study, the difference in gene expression in Flammulina velutipes was compared between the wild type (WT) mushroom and the mutant one with clogging phenomenon. Differentially expressed transcripts were screened to identify the candidate genes responsible for the mutant phenotype using the DNA microarray analysis. A total of 88 genes including 60 upregulated and 28 downregulated genes were validated using the real-time quantitative PCR analysis. In addition, proteomic differences between the WT and mutant mushroom were analyzed using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF). Interestingly, the genes identified by these genomic and proteomic analyses were involved in stress response, translation, and energy/sugar metabolism, including HSP70, elongation factor 2, and pyruvate kinase. Together, our data suggest that the aberrant expression of these genes attributes to the mutant clogging phenotype. We propose that these genes can be targeted to foster normal growth in F. velutipes.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/36/cb/TMYB_50_2121497.PMC9645268.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40485765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Re-Identification on Korean Penicillium Sequences in GenBank Collected by Software GenMine. GenMine软件收集的韩国青霉菌基因库序列再鉴定。

IF 1.9 4区生物学

Mycobiology Pub Date : 2022-09-05 eCollection Date: 2022-01-01 DOI: 10.1080/12298093.2022.2116816

Chang Wan Seo, Sung Hyun Kim, Young Woon Lim, Myung Soo Park

{"title":"Re-Identification on Korean Penicillium Sequences in GenBank Collected by Software GenMine.","authors":"Chang Wan Seo, Sung Hyun Kim, Young Woon Lim, Myung Soo Park","doi":"10.1080/12298093.2022.2116816","DOIUrl":"https://doi.org/10.1080/12298093.2022.2116816","url":null,"abstract":"Penicillium species have been actively studied in various fields, and many new and unrecorded species continue to be reported in Korea. Moreover, unidentified and misidentified Korean Penicillium species still exist in GenBank. Therefore, it is necessary to revise the Korean Penicillium inventory based on accurate identification. We collected Korean Penicillium nucleotide sequence records from GenBank using the newly developed software, GenMine, and re-identified Korean Penicillium based on the maximum likelihood trees. A total of 1681 Korean Penicillium GenBank nucleotide sequence records were collected from GenBank. In these records, 1208 strains with four major genes (Internal Transcribed Spacer rDNA region, β-tubulin, Calmodulin and RNA polymerase II) were selected for Penicillium re-identification. Among 1208 strains, 927 were identified, 82 were identified as other genera, the rest remained undetermined due to low phylogenetic resolution. Identified strains consisted of 206 Penicillium species, including 156 recorded species and 50 new species candidates. However, 37 species recorded in the national list of species in Korea were not found in GenBank. Further studies on the presence or absence of these species are required through literature investigation, additional sampling, and sequencing. Our study can be the basis for updating the Korean Penicillium inventory.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/45/1a/TMYB_50_2116816.PMC9467555.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33482490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seven Undescribed Aspergillus Species from Different Niches in Korea. 韩国不同生态位的7种未描述曲霉。

IF 1.9 4区生物学

Mycobiology Pub Date : 2022-09-05 eCollection Date: 2022-01-01 DOI: 10.1080/12298093.2022.2116158

Monmi Pangging, Thuong T T Nguyen, Hyang Burm Lee

引用次数: 4

Effect of Temperature, pH, and Media on the Mycelial Growth of Tuber koreanum. 温度、pH和培养基对韩国块茎菌丝生长的影响。

IF 1.9 4区生物学

Mycobiology Pub Date : 2022-09-01 eCollection Date: 2022-01-01 DOI: 10.1080/12298093.2022.2112586

Ju-Hui Gwon, Hyeok Park, Ahn-Heum Eom

引用次数: 1

Seven Unrecorded Indigenous Fungi from Mudeungsan National Park in Korea. 韩国无登山国立公园的7种未记录的本土真菌。

IF 1.9 4区生物学

Mycobiology Pub Date : 2022-08-25 eCollection Date: 2022-01-01 DOI: 10.1080/12298093.2022.2109269

Minseo Cho, Sun Lul Kwon, Young Mok Heo, Young Min Lee, Hanbyul Lee, Changmu Kim, Byoung Jun Ahn, Jae-Jin Kim

引用次数: 0

Characterization of Chitinolytic and Antifungal Activities in Marine-Derived Trichoderma bissettii Strains. 海洋衍生毛霉菌株的几丁质溶解和抗真菌活性表征

IF 1.6 4区生物学

Mycobiology Pub Date : 2022-08-24 eCollection Date: 2022-01-01 DOI: 10.1080/12298093.2022.2105509

Dawoon Chung, Yong Min Kwon, Ji Yeon Lim, Seung Sub Bae, Grace Choi, Dae-Sung Lee

{"title":"Characterization of Chitinolytic and Antifungal Activities in Marine-Derived Trichoderma bissettii Strains.","authors":"Dawoon Chung, Yong Min Kwon, Ji Yeon Lim, Seung Sub Bae, Grace Choi, Dae-Sung Lee","doi":"10.1080/12298093.2022.2105509","DOIUrl":"10.1080/12298093.2022.2105509","url":null,"abstract":"Trichoderma fungi have been intensively studied for mycoparasitism, and the latter is closely related to their cell-wall degrading enzymes including chitinase. Here, we studied marine-derived Trichoderma spp., isolated from distinct sources and locations, for chitinolytic and antifungal activity. Based on morphological and phylogenetic analyses, two strains designated GJ-Sp1 and TOP-Co8 (isolated from a marine sponge and a marine alga, respectively) were identified as Trichoderma bissettii. This species has recently been identified as a closely related species to Trichoderma longibrachiatum. The extracellular crude enzymes of GJ-Sp1 and TOP-Co8 showed activities of chitobiosidase and β-N-acetylglucosaminidase (exochitinase) and chitotriosidase (endochitinase). The optimum chitinolytic activity of the crude enzymes was observed at 50 °C, pH 5.0, 0-0.5% NaCl concentrations, and the activities were stable at temperatures ranging from 10 to 40 °C for 2 h. Moreover, the crude enzymes showed inhibitory activity against hyphal growth of two filamentous fungi Aspergillus flavus and Aspergillus niger. To the best of our knowledge, this is the first report of the chitinolytic and antifungal activity of T. bissettii.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/51/38/TMYB_50_2105509.PMC9467547.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33482920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Draft Genome Sequence of the Reference Strain of the Korean Medicinal Mushroom Wolfiporia cocos KMCC03342. 韩国药用菌cocos Wolfiporia KMCC03342参考菌株基因组序列草图

IF 1.9 4区生物学

Mycobiology Pub Date : 2022-08-24 eCollection Date: 2022-01-01 DOI: 10.1080/12298093.2022.2109874

Bogun Kim, Byoungnam Min, Jae-Gu Han, Hongjae Park, Seungwoo Baek, Subin Jeong, In-Geol Choi

{"title":"Draft Genome Sequence of the Reference Strain of the Korean Medicinal Mushroom Wolfiporia cocos KMCC03342.","authors":"Bogun Kim, Byoungnam Min, Jae-Gu Han, Hongjae Park, Seungwoo Baek, Subin Jeong, In-Geol Choi","doi":"10.1080/12298093.2022.2109874","DOIUrl":"https://doi.org/10.1080/12298093.2022.2109874","url":null,"abstract":"Wolfiporia cocos is a wood-decay brown rot fungus belonging to the family Polyporaceae. While the fungus grows, the sclerotium body of the strain, dubbed Bokryeong in Korean, is formed around the roots of conifer trees. The dried sclerotium has been widely used as a key component of many medicinal recipes in East Asia. Wolfiporia cocos strain KMCC03342 is the reference strain registered and maintained by the Korea Seed and Variety Service for commercial uses. Here, we present the first draft genome sequence of W. cocos KMCC03342 using a hybrid assembly technique combining both short- and long-read sequences. The genome has a total length of 55.5 Mb comprised of 343 contigs with N50 of 332 kb and 95.8% BUSCO completeness. The GC ratio was 52.2%. We predicted 14,296 protein-coding gene models based on ab initio gene prediction and evidence-based annotation procedure using RNAseq data. The annotated genome was predicted to have 19 terpene biosynthesis gene clusters, which was the same number as the previously sequenced W. cocos strain MD-104 genome but higher than Chinese W. cocos strains. The genome sequence and the predicted gene clusters allow us to study biosynthetic pathways for the active ingredients of W. cocos.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/58/60/TMYB_50_2109874.PMC9467534.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33482921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Fourteen Unrecorded Species of Agaricales Underw. (Agaricomycetes, Basidiomycota) from the Republic of Korea. 来自大韩民国的 14 个未记录的姬松茸物种（姬松茸目，担子菌科）。(Agaricomycetes, Basidiomycota) from the Republic of Korea.

IF 1.6 4区生物学

Mycobiology Pub Date : 2022-08-09 eCollection Date: 2022-01-01 DOI: 10.1080/12298093.2022.2097364

Shinnam Yoo, Yoonhee Cho, Ji Seon Kim, Minkyeong Kim, Young Woon Lim

{"title":"Fourteen Unrecorded Species of Agaricales Underw. (Agaricomycetes, Basidiomycota) from the Republic of Korea.","authors":"Shinnam Yoo, Yoonhee Cho, Ji Seon Kim, Minkyeong Kim, Young Woon Lim","doi":"10.1080/12298093.2022.2097364","DOIUrl":"10.1080/12298093.2022.2097364","url":null,"abstract":"Agaricales species form pileate-stipitate fruiting bodies and play important roles in maintaining the terrestrial ecosystem as decomposers, symbionts, and pathogens. Approximately 23,000 Agaricales species have been known worldwide, and 937 species have been recorded in the Republic of Korea. However, most of them were identified solely based on morphological characteristics that often led to misidentifications. The specimens collected from 2018 to 2020 in the Republic of Korea were identified based on phylogenetic analysis of the internal transcribed spacer (ITS) sequences. Their identities were confirmed by microscopic characteristics. As a result, 14 Agaricales species were discovered for the first time in the Republic of Korea. They belonged to nine genera: Agaricus, Calocybe, Cortinarius, Hygrocybe, Inocybe, Lepista, Leucoagaricus, Marasmius, and Psathyrella. Detailed macroscopic and microscopic descriptions were provided to help distinguish these species. The morphological and molecular data provided in this study will serve as reliable references for the identification of Agaricales species.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b4/12/TMYB_50_2097364.PMC9467565.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33482487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0