Mycobiology最新文献

{"title":"<i>Humicola ovoideum</i> sp. nov. and <i>Pseudohumicola obscura</i> sp. nov., Two Novel Chaetomiaceae Species Reported in Korea.","authors":"Hae-Dam Kim, Mohammad Hamizan Azmi, Seong-Keun Lim, Gang-Won Jeong, Seung-Yeol Lee, Hee-Young Jung","doi":"10.1080/12298093.2026.2696652","DOIUrl":"10.1080/12298093.2026.2696652","url":null,"abstract":"<p><p>During an investigation of soil micro-fungi in Korea, two fungal strains designated as strains KNU-1816^T and KNUF-20-124^T were isolated from soil samples collected from Gyeongbuk province. The morphological features of strain KNU-1816^T included obovate greenish-yellow ascomata with bristle-like dark-brown hairs and clavate asci with 8 biseriate bilaterally flattened limoniform olivaceous brown ascospores containing an apical germ pore and subhyaline or pale brown conidia. While strain KNUF-20-124^T produced aleurioconidia-like conidia and hyaline, <i>Acremonium</i>-like conidiophores. Phylogenetic analyses based on the concatenated nucleotide sequences of the internal transcribed spacer (ITS) regions, partial sequences of the large subunit (<i>LSU</i>) 28S rRNA, RNA polymerase II largest subunit (<i>RPB</i>2), and beta-tubulin (<i>TUB</i>2) placed strains KNU-1816^T and KNUF-20-124^T within the family Chaetomiaceae, specifically in the genera <i>Humicola</i> and <i>Pseudohumicola</i>, respectively. Each occupied a distinct phylogenetic position separate from known species, thereby warranting their designation as novel species. Here, we describe and illustrate two new species, <i>Humicola ovoideum</i> sp. nov. KNU-1816^T and <i>Pseudohumicola obscura</i> sp. nov. KNUF-20-124^T, providing detailed morphological descriptions and phylogenetic insights.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 4","pages":"441-452"},"PeriodicalIF":2.5,"publicationDate":"2026-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13371474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148456501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arbuscular Mycorrhizal Fungal Community Shifts Driven by Heavy Metals in Cocoa Agroecosystems at Different Crop Age.","authors":"Cecil Eduardo Del Aguila-Torres, Maria Nelly Pezo-Torres, Sergio Sebastian Vega-Herrera, Carlos Verde-Girbau, Enrique Meseth, Mike Anderson Corazon-Guivin, Jorge Luis Paz-Urrelo","doi":"10.1080/12298093.2026.2688575","DOIUrl":"10.1080/12298093.2026.2688575","url":null,"abstract":"<p><p>Cocoa (<i>Theobroma cacao</i> L.) is an economically important tropical crop, but its production is increasingly constrained by strict regulations related to heavy metal accumulation in soils. Arbuscular mycorrhizal fungi (AMF) are a key biological component of cocoa agroecosystems due to their role in soil processes and plant nutrition. However, information on AMF diversity and their response to heavy metals remains limited. In this study, AMF communities were evaluated in cocoa plantations of different ages established under contrasting heavy metal conditions. A total of 53 AMF taxa were identified, indicating high fungal richness across all plantations. No significant differences were found in α-diversity indices or community composition among plantation ages. In contrast, multivariate analyses showed that variation in AMF community structure was primarily associated with soil heavy metal gradients, particularly cadmium (Cd) and zinc (Zn). Species-level models revealed contrasting responses among taxa, with both positive and negative associations along Cd and Zn gradients, suggesting differential tolerance strategies. Overall, these results indicate that edaphic factors, especially heavy metal concentrations, play a more important role than plantation age in shaping AMF community composition. These findings provide a basis for identifying AMF taxa adapted to metal-enriched soils and for developing management strategies that support sustainable cocoa production.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 3","pages":"424-439"},"PeriodicalIF":2.5,"publicationDate":"2026-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13347917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148422714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ice Nucleation Activity by <i>Fusarium tricinctum</i> Can Enhance Cold Stress: A Potential Contributor to Cold Injury in Apple Trees.","authors":"Jin-Sil Choi, Aung Htay Naing, Diane Avalos-Ruiz, Jova Riza Campol, Seong-Keun Lim, In-Kyu Kang, Chang-Kil Kim, Seung-Yeol Lee, Hee-Young Jung","doi":"10.1080/12298093.2026.2687921","DOIUrl":"10.1080/12298093.2026.2687921","url":null,"abstract":"<p><p>Cold stress is a major abiotic factor that adversely affects apple trees and associated dwarf rootstocks, increasing the susceptibility of apple trees and dwarf rootstocks to secondary biotic infections. Among these biotic factors, certain <i>Fusarium</i> species exhibit ice nucleation activity (INA), which can exacerbate cold injury. Therefore, this study aimed to evaluate the effects of INA-positive <i>Fusarium tricinctum</i> KNUF-21-F29 and INA-negative <i>F. solani</i> KNUF-21-F27 on the growth of <i>in vitro</i> apple dwarf rootstocks (cultivars Malling 9 and Malling 26). Apple shoots treated with KNUF-21-F29 culture filtrates and exposed to low temperatures showed significantly reduced growth compared with those treated with KNUF-21-F27, suggesting that the INA of <i>F. tricinctum</i> contributes to growth inhibition. Gene expression analysis revealed that KNUF-21-F29 filtrates upregulated genes associated with ethylene biosynthesis, antioxidant activity, and cold response. Furthermore, Malling 9 rootstocks treated with KNUF-21-F29 filtrates for 3 months had lower survival rates than both potato dextrose broth (PDB)-treated and untreated controls. These findings demonstrate that fungal INA can negatively affect apple growth and represent a critical biotic factor in the development of cold injury.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 3","pages":"396-406"},"PeriodicalIF":2.5,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13347845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148422761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taxonomy, Host Associations, and Life Cycles of Four Rust Fungi Associated with <i>Prunus</i> spp. in Korea.","authors":"Yoo Kyeong Lee, Jae Sung Lee, Seong-Jin Lee, Seung-Yeol Lee, Hee-Young Jung, Yongho Jeon, Hyeon-Dong Shin, Young-Joon Choi","doi":"10.1080/12298093.2026.2688579","DOIUrl":"10.1080/12298093.2026.2688579","url":null,"abstract":"<p><p>Rust fungi associated with <i>Prunus</i> spp. in Korea have been reported under different genera, yet their taxonomy, host associations, and life cycles have remained insufficiently clarified. In this study, rust specimens collected from <i>Prunus</i> species and their putative alternate hosts were examined using morphological observations and molecular phylogenetic analyses based on internal transcribed spacer (ITS) and large subunit (LSU) rDNA sequences. Four species belonging to three genera were identified: <i>Blastospora makinoi</i>, <i>Blastospora radiata</i>, <i>Leucotelium semiaquilegiae</i>, and <i>Tranzschelia discolor. Blastospora radiata</i> and <i>L. semiaquilegiae</i> are newly recorded in Korea. In addition, <i>Prunus salicina</i> and <i>Semiaquilegia adoxoides</i> are reported for the first time in Korea as hosts of rust fungi. Field investigations combined with sequence data clarified the heteroecious life cycles of <i>Blastospora</i> and <i>Leucotelium</i> species in Korea, confirming host alternation between <i>Prunus</i> spp. and alternate hosts including <i>Se. adoxoides</i> and <i>Smilax</i> spp. Historical records of rust fungi on <i>Prunus</i> in South Korea were taxonomically reassessed in light of these results. The congruence between molecular phylogenies and morphological characteristics provides an updated taxonomic framework for rust fungi associated with economically important <i>Prunus</i> species.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 3","pages":"407-423"},"PeriodicalIF":2.5,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13347839/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148422724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole Genome Sequence of <i>Xylaria chamelensis</i> (Xylariales, Ascomycota), A New Species Associated with Fabaceae Pods.","authors":"Yulena Osorio-Navarro, Ricardo Valenzuela, César Hernández-Rodríguez, Tania Raymundo, Abigail Pérez-Valdespino, Hannya Chamorro-Martínez, Juan Alfredo Hernández-García","doi":"10.1080/12298093.2026.2675762","DOIUrl":"10.1080/12298093.2026.2675762","url":null,"abstract":"<p><p>The cosmopolitan genus <i>Xylaria</i> Hill ex Schrank (Ascomycota, Sordariomycetes), is the largest within the order Xylariales. While <i>Xylaria</i> species primarily exist as saprotrophs, they can also occur as endophytes or as phytopathogens. Specimens of <i>Xylaria</i> sp. were collected from a Fabaceae pod in the tropical dry forest in the state of Jalisco, Mexico. They were isolated and axenized in PDA culture medium. The genome of the strain was sequenced using Illumina NovaSeq technology and assembled. A phylogenomic approach was used to confirm its taxonomic position within the genus <i>Xylaria</i>. The resulting genome assembly is 51.93 Mb in size, featuring an N50 of 452,556 bp and a G + C content of 42.34%. Gene prediction with AUGUSTUS identified 11,238 protein-coding genes. Based on morphological and genomic evidence, these specimens are described here as a new species, <i>Xylaria chamelensis</i> sp. nov. Phylogenomic analysis incorporating available <i>Xylaria</i> genomes revealed that this strain forms a distinct, independent lineage within the <i>Xylaria</i> phylogenetic tree. Ultimately, these genomic data provide the scientific community with a valuable resource to advance genome-based taxonomy, unravel the molecular basis of plant-fungus associations, and further explore the diverse metabolism of the genus <i>Xylaria</i> across tropical and subtropical environments.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 3","pages":"384-395"},"PeriodicalIF":2.5,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13270877/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148272321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Conserved Lignocellulolytic Secretome with Quantitative Modulation in <i>Leucoagaricus gongylophorus</i>, the Mutualistic Fungus of Leaf-Cutting Ants.","authors":"Gabriela Cejas-Añón, Freddy Castillo-Alfonso, Ulises Carrasco-Navarro, Jorge Valenzuela-González, Roberto Olivares-Hernández, Gabriel Vigueras-Ramírez","doi":"10.1080/12298093.2026.2680805","DOIUrl":"10.1080/12298093.2026.2680805","url":null,"abstract":"<p><p><i>Leucoagaricus gongylophorus</i> is essential to the mutualistic relationship between leaf-cutting ants and their fungal gardens. Known for its ability to degrade complex plant polysaccharides, this fungus produces a range of CAZymes and FOLymes enzymes that have significant potential for biotechnological applications. We investigated the growth kinetics, enzymatic activities and quantitative secretome modulation of <i>L. gongylophorus</i> grown in submerged cultures containing glucose, carboxymethylcellulose (CMC), xylan or pectin as sole carbon sources. In the comparative analysis, glucose was the preferred carbon source for biomass accumulation reaching 8.6 g L^-1 at day 35, whereas cultures grown on pectin showed limited growth. In the case of enzyme activity, pectin cultures had the highest values in endo-<i>β</i>-1,4-glucanase, pectinase and laccase activities, reaching up to 50.7 U L^-1, 206.6 U L^-1 and 97.3 U L^-1, respectively. In addition, a quantitative proteomic analysis was performed across the different growth conditions and identified 290 proteins, of which 201 were assigned to the secretome. Presence-absence analysis revealed 93 proteins shared across substrates, including 43 consistently detected at both time points, while pectin cultures exhibiting a more diverse set of exclusive secreted proteins. A key difference between conditions was in protein abundance rather in terms of protein profiles, cellulolytic enzymes were preferentially enriched in CMC cultures, pectinolytic enzymes in pectin, and oxidative proteins in xylan-grown cultures. Based on our results, <i>L. gongylophorus</i> adapts to different polysaccharides primarily through quantitative regulation of a shared secretome, reflecting a finely tuned enzymatic strategy that is consistent with its mutualistic lifestyle.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 3","pages":"371-383"},"PeriodicalIF":2.5,"publicationDate":"2026-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13244510/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148211911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Species and Record of <i>Erioscyphella</i> (Lachnaceae, Helotiales) from Korea.","authors":"Dae Ho Kim, Young-Nam Kwag, Hyung So Kim, Chang-Sun Kim, Sang-Kuk Han","doi":"10.1080/12298093.2026.2674375","DOIUrl":"10.1080/12298093.2026.2674375","url":null,"abstract":"<p><p>The genus <i>Erioscyphella</i> (Lachnaceae, Helotiales), characterized by minute apothecia, is distributed in subtropical regions of East Asia; however, relatively little is known about species in Korea. In this study, Korean collections of <i>Erioscyphella</i> were examined using morphological observations and multilocus phylogenetic analyses based on ITS, LSU, and RPB2 sequences. One new taxon, <i>Erioscyphella koreana</i> sp. nov., has been described, and <i>Erioscyphella hainanensis</i> has been newly recorded in Korea. The Korean specimens of <i>E. hainanensis</i> showed similar morphological characteristics to those of previously reported <i>E. hainanensis</i> and high sequence similarity in the combined ITS-LSU-RPB2 analysis. In the multilocus phylogeny, <i>E. koreana</i> was recovered as a distinct clade closely related to <i>Erioscyphella boninensis</i>, <i>Erioscyphella lushanensis</i>, and <i>Erioscyphella paralushanensis</i>. However, it was distinguishable from these species by a combination of morphological characteristics, including apothecial coloration, ascus morphology, and ascospore dimensions. Korean <i>Erioscyphella abnormis</i>, <i>E. hainanensis</i>, and <i>E. koreana</i> shared similar apothecial structures but differed in ascospore septation, paraphysis morphology, and substrate preference. These findings expand our knowledge of <i>Erioscyphella</i> diversity in Korea and highlight the value of integrative taxonomy for species delimitation.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 3","pages":"362-370"},"PeriodicalIF":2.5,"publicationDate":"2026-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13235234/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Transcriptome Analysis of <i>Xylaria grammica</i> Under Light and Dark Conditions Reveals Dark-Responsive Activation of a Patulin-like Locus Involved in Grammicin Biosynthesis.","authors":"Yebin Nam, Yoeguang Hue, Sunmin An, Jongbum Jeon, Jinyong Kim, Sook-Young Park, Soonok Kim, Won-Jae Chi, Ki-Tae Kim","doi":"10.1080/12298093.2026.2674329","DOIUrl":"10.1080/12298093.2026.2674329","url":null,"abstract":"<p><p><i>Xylaria grammica</i> is an endophytic fungus that produces grammicin, a polyketide reported as a structural isomer of the mycotoxin patulin. However, the environmental regulation of grammicin production remains unclear, and the full biosynthetic pathway has not yet been completely resolved. Genome re-annotation and biosynthetic gene cluster mining revealed high secondary metabolite potential, with 92 predicted clusters spanning major biosynthetic classes. Time-series RNA sequencing under shaking dark, static dark, and continuous light conditions showed strong condition-dependent transcriptome divergence. Light-grown cultures were enriched in functions associated with stress adaptation and detoxification, including oxidoreductase- and monooxygenase-related categories, whereas static dark cultures showed broader activation of secondary metabolism-associated genes at later stages. Among the predicted biosynthetic gene clusters, a patulin-like locus in <i>X. grammica</i> contained homologs corresponding to most genes of the <i>Aspergillus clavatus pat</i> cluster from <i>PatA</i> to <i>PatO</i>, although the gene order differed. Expression profiling revealed coordinated induction of patulin-like genes after 3 days post-inoculation under dark conditions, whereas continuous light strongly repressed their transcription. Quantitative RT-PCR analysis further confirmed this darkness-dependent activation pattern. Together, with previous functional evidence showing that <i>patK</i> and <i>patL</i> are required for grammicin production, these results indicate that darkness acts as a major regulatory switch for secondary metabolism in <i>X. grammica</i> and support the involvement of a patulin-like locus in grammicin biosynthesis. However, the downstream tailoring steps leading to grammicin remain unresolved, and further genetic and metabolomic analyses will be required to complete pathway reconstruction.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 3","pages":"346-361"},"PeriodicalIF":2.5,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13215408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148055813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arbuscular Mycorrhizal Fungi Inoculation Mediated Changes in Phytohormones Under Drought Stress.","authors":"Murugesan Chandrasekaran, Iyyakkannu Sivanesan, Se Chul Chun","doi":"10.1080/12298093.2026.2670137","DOIUrl":"10.1080/12298093.2026.2670137","url":null,"abstract":"<p><p>Changes in climatic conditions around the world have increased day by day which leads to droughts in agricultural lands. Nowadays various technologies and methodologies are being used to protect plants from drought stress. Among various methods, arbuscular mycorrhizal fungi (AMF) mediated alleviation of drought stress was found to be a more efficient one. Most of the AMF studies revealed their importance for water status, gas exchange, and nutrient uptake, but they also act as potential candidates for the regulation of phytohormones. At present, there is no comprehensive and quantitative evaluation of the phytohormonal changes under drought stress. Therefore, we carried out a random-effects model of meta-analysis to explore the influences of AMF inoculation on phytohormones under drought stress. Results showed that stress hormones increased by 2% but were non-significant, whereas growth hormones significantly increased by 24% in AMF-inoculated plants. Among phytohormones, indole acetic acid (IAA) content increased significantly by 37%. Also increased content of jasmonic acid (JA) (25%), salicylic acid (SA) (25%), gibberellins (24%), and brassinosteroids (11%) compared to non-mycorrhizal plants. Due to low sample size, we treated results with caution. However, abscisic acid (ABA) contents decreased by 6% in AMF-inoculated plants. Among growth hormones, IAA showed more positive effect sizes in AMF-inoculated plants under drought stress conditions. Among phytohormones, JA and SA had a positive effect size, whereas ABA showed a negative effect size. These results conclude that depending upon the level of stress, AMF species and plant species determined the accumulation of phytohormones under drought stress.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 3","pages":"336-345"},"PeriodicalIF":2.5,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13202652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148043046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two New Species of <i>Collybiopsis</i> (Omphalotaceae, Agaricales) from Southwestern Guizhou, China.","authors":"Heng-Ting Chen, Zhu Guo-Sheng, Yang Tong-Jing, Li Biao, Gong Guang-Lu, Huang Wang-Bing, Shang Nian-Jie, Gui Yang","doi":"10.1080/12298093.2026.2668165","DOIUrl":"10.1080/12298093.2026.2668165","url":null,"abstract":"<p><p>Two new species of <i>Collybiopsis</i>, <i>Co. guizhouensis</i> and <i>Co. anlongensis</i>, are described from southwestern Guizhou Province, China, based on morphological and multi-gene phylogenetic analyses (internal transcribed spacer (ITS), nuclear large subunit ribosomal DNA (nrLSU), and translation elongation factor 1-α (<i>tef1-α</i>)). Phylogenetic analyses (ML/BI) of the combined ITS-nrLSU-<i>tef1-α</i> dataset placed each species within a distinct and well-supported clade: <i>Co. guizhouensis</i> (ML-BP = 100%; BI-PP = 0.94) and <i>Co. anlongensis</i> (ML-BP = 100%; BI-PP = 1.00). These molecular results, together with morphological distinctions, support their recognition as new species.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 3","pages":"326-335"},"PeriodicalIF":2.5,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13202682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148043064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}