伽玛辐射处理黑木耳子实体菌丝体的转录分析。

IF 2 4区 生物学 Q2 AGRONOMY
Mycobiology Pub Date : 2025-08-03 eCollection Date: 2025-01-01 DOI:10.1080/12298093.2025.2532236
Jeong-Heon Kim, Youn-Jin Park, Myoung-Jun Jang
{"title":"伽玛辐射处理黑木耳子实体菌丝体的转录分析。","authors":"Jeong-Heon Kim, Youn-Jin Park, Myoung-Jun Jang","doi":"10.1080/12298093.2025.2532236","DOIUrl":null,"url":null,"abstract":"<p><p>Fungi have a high resistance to gamma radiation. Previous studies elucidating the resistance mechanisms to gamma radiation have mainly been conducted using yeast model organisms, but molecular-level mechanisms targeting fungi have been largely unexplored. In order to examine the response of <i>A. auricula-judae</i> to gamma radiation, we conducted comparative transcriptome analysis using RNA-Seq on fruit bodies derived from mycelium exposed with 200 Gray of gamma radiation. A total of 69,183 unigenes were generated, and annotation was performed through two libraries. According to GO analysis, catalytic activity, cell part, and cellular process groups were identified as the major groups responding to gamma radiation. Differentially expressed genes revealed inhibited expression in amino acid metabolism, carbohydrate metabolism, and cell cycle regulation genes. Furthermore, enhanced cell death promoted resistance by eliminating unstable cells. DNA repair genes were up-regulated, indicating a common defense mechanism of fungi that increases genome integrity by repairing DNA damaged by gamma radiation. Conversely, genes related to antioxidants and laccase were down-regulated, suggesting that resistance response through resolving oxidative stress induced by gamma radiation did not occur. Our study provides valuable data for understanding not only the response of <i>A. auricula-judae</i> to gamma radiation but also the resistance responses of edible mushrooms to gamma radiation.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"53 5","pages":"595-604"},"PeriodicalIF":2.0000,"publicationDate":"2025-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12322991/pdf/","citationCount":"0","resultStr":"{\"title\":\"Transcriptom Analysis of <i>Auricularia auriculla-judae</i> Fruit Body Treated with Gamma Radiation on Mycelium.\",\"authors\":\"Jeong-Heon Kim, Youn-Jin Park, Myoung-Jun Jang\",\"doi\":\"10.1080/12298093.2025.2532236\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Fungi have a high resistance to gamma radiation. Previous studies elucidating the resistance mechanisms to gamma radiation have mainly been conducted using yeast model organisms, but molecular-level mechanisms targeting fungi have been largely unexplored. In order to examine the response of <i>A. auricula-judae</i> to gamma radiation, we conducted comparative transcriptome analysis using RNA-Seq on fruit bodies derived from mycelium exposed with 200 Gray of gamma radiation. A total of 69,183 unigenes were generated, and annotation was performed through two libraries. According to GO analysis, catalytic activity, cell part, and cellular process groups were identified as the major groups responding to gamma radiation. Differentially expressed genes revealed inhibited expression in amino acid metabolism, carbohydrate metabolism, and cell cycle regulation genes. Furthermore, enhanced cell death promoted resistance by eliminating unstable cells. DNA repair genes were up-regulated, indicating a common defense mechanism of fungi that increases genome integrity by repairing DNA damaged by gamma radiation. Conversely, genes related to antioxidants and laccase were down-regulated, suggesting that resistance response through resolving oxidative stress induced by gamma radiation did not occur. Our study provides valuable data for understanding not only the response of <i>A. auricula-judae</i> to gamma radiation but also the resistance responses of edible mushrooms to gamma radiation.</p>\",\"PeriodicalId\":18825,\"journal\":{\"name\":\"Mycobiology\",\"volume\":\"53 5\",\"pages\":\"595-604\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-08-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12322991/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mycobiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/12298093.2025.2532236\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycobiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/12298093.2025.2532236","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0

摘要

真菌对伽马射线有很强的抵抗力。以前的研究主要是利用酵母模式生物来阐明对伽马辐射的抗性机制,但针对真菌的分子水平机制在很大程度上尚未探索。为了研究A. auricula-judae对γ辐射的反应,我们利用RNA-Seq方法对辐照200 Gray γ辐射的菌丝体进行了转录组比较分析。总共生成了69,183个unigenes,并通过两个库进行了注释。根据氧化石墨烯分析,催化活性、细胞部分和细胞过程组被确定为对伽马辐射有反应的主要组。差异表达基因显示氨基酸代谢、碳水化合物代谢和细胞周期调控基因的表达受到抑制。此外,细胞死亡的增强通过消除不稳定的细胞来促进耐药性。DNA修复基因被上调,表明真菌通过修复伽马辐射损伤的DNA来提高基因组完整性的共同防御机制。相反,与抗氧化剂和漆酶相关的基因被下调,表明通过解决伽马辐射诱导的氧化应激而产生的抗性反应没有发生。本研究不仅为了解auricula-judae对γ辐射的反应,也为了解食用菌对γ辐射的抗性反应提供了有价值的数据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Transcriptom Analysis of <i>Auricularia auriculla-judae</i> Fruit Body Treated with Gamma Radiation on Mycelium.

Transcriptom Analysis of <i>Auricularia auriculla-judae</i> Fruit Body Treated with Gamma Radiation on Mycelium.

Transcriptom Analysis of <i>Auricularia auriculla-judae</i> Fruit Body Treated with Gamma Radiation on Mycelium.

Transcriptom Analysis of Auricularia auriculla-judae Fruit Body Treated with Gamma Radiation on Mycelium.

Fungi have a high resistance to gamma radiation. Previous studies elucidating the resistance mechanisms to gamma radiation have mainly been conducted using yeast model organisms, but molecular-level mechanisms targeting fungi have been largely unexplored. In order to examine the response of A. auricula-judae to gamma radiation, we conducted comparative transcriptome analysis using RNA-Seq on fruit bodies derived from mycelium exposed with 200 Gray of gamma radiation. A total of 69,183 unigenes were generated, and annotation was performed through two libraries. According to GO analysis, catalytic activity, cell part, and cellular process groups were identified as the major groups responding to gamma radiation. Differentially expressed genes revealed inhibited expression in amino acid metabolism, carbohydrate metabolism, and cell cycle regulation genes. Furthermore, enhanced cell death promoted resistance by eliminating unstable cells. DNA repair genes were up-regulated, indicating a common defense mechanism of fungi that increases genome integrity by repairing DNA damaged by gamma radiation. Conversely, genes related to antioxidants and laccase were down-regulated, suggesting that resistance response through resolving oxidative stress induced by gamma radiation did not occur. Our study provides valuable data for understanding not only the response of A. auricula-judae to gamma radiation but also the resistance responses of edible mushrooms to gamma radiation.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Mycobiology
Mycobiology AGRONOMYMYCOLOGY-MYCOLOGY
CiteScore
3.90
自引率
5.30%
发文量
41
审稿时长
22 weeks
期刊介绍: Mycobiology is an international journal devoted to the publication of fundamental and applied investigations on all aspects of mycology and their traditional allies. It is published quarterly and is the official publication of the Korean Society of Mycology. Mycobiology publishes reports of basic research on fungi and fungus-like organisms, including yeasts, filamentous fungi, lichen fungi, oomycetes, moulds, and mushroom. Topics also include molecular and cellular biology, biochemistry, metabolism, developmental biology, environmental mycology, evolution, ecology, taxonomy and systematics, genetics/genomics, fungal pathogen and disease control, physiology, and industrial biotechnology using fungi.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信