在烧瓶和生物反应器条件下产螨致病性枯黄赤色菌JEF-147胚孢子。

IF 2 4区 生物学 Q2 AGRONOMY
Mycobiology Pub Date : 2025-07-23 eCollection Date: 2025-01-01 DOI:10.1080/12298093.2025.2532235
Gahyeon Song, Mi Rong Lee, Jae Su Kim
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引用次数: 0

摘要

采用液体培养的方法制备了具有螨致病性的Akanthomyces attenuatus JEF-147胚芽孢子,并将其作为制剂的活性成分,这是一种比目前使用的固体培养方法更实用、更经济的方法,但抗病性仍是一个挑战。本研究首先采用淀粉或葡萄糖为基础的人工液体培养基,在烧瓶条件下培养具有高杀螨活性的JEF-147。淀粉基培养基产生的胚孢子数量明显高于葡萄糖基培养基。葡萄糖基培养基中的JEF-147胚孢子比淀粉基培养基中的JEF-147胚孢子发病更快。该液体培养在2.5 l生物反应器中按比例放大,葡萄糖基培养基和1.0 v/v/m供气。接种后36 h开始产生胚孢子,但在94 h也未观察到活跃的胚孢子产生。或者,在相同的生物反应器条件下,以葡萄糖为基础的培养基中添加1% (w/w)的硫酸镁,可以产生大量的胚孢子。本研究表明,葡萄糖基液体培养的JEF-147芽孢可用于螨病防治,在培养基中添加盐可能确保芽孢在规模化条件下产生。在规模化条件下对盐和培养参数进行具体优化,并进行实地研究,将是下一步的重要工作。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Production of Mite-Pathogenic <i>Akanthomyces attenuatus</i> JEF-147 Blastospores in Flask and Bioreactor Conditions.

Production of Mite-Pathogenic <i>Akanthomyces attenuatus</i> JEF-147 Blastospores in Flask and Bioreactor Conditions.

Production of Mite-Pathogenic <i>Akanthomyces attenuatus</i> JEF-147 Blastospores in Flask and Bioreactor Conditions.

Production of Mite-Pathogenic Akanthomyces attenuatus JEF-147 Blastospores in Flask and Bioreactor Conditions.

Mite-pathogenic Akanthomyces attenuatus JEF-147 was liquid-cultured to produce blastospores as an active ingredient of formulations, which is more practical and cost-effective method than currently used solid cultures although stress resistance remains a challenge. In this work, first starch- or glucose-based artificial liquid culture media were used to culture JEF-147 with high miticidal activity against two-spotted spider mites (Tetranychus urticae) in flask conditions. The starch-based medium produced significantly higher numbers of blastospores than the glucose-based medium. JEF-147 blastospores from the glucose-based medium showed faster pathogenesis than those from starch-based medium. This liquid cultured was scaled up in a 2.5-L bioreactor with the glucose-based medium and 1.0 v/v/m of air supply. Production of blastospores was initiated 36 h after inoculation, but active blastospore production was not observed even in 94 h. Alternatively, the glucose-based medium was supplemented with magnesium sulfate at 1% (w/w) in the same bioreactor conditions, and it produced large number of blastospores. This work suggests that JEF-147 blastospores from the glucose-based liquid culture could be used for mite control and the supplement of salt to the medium possibly ensures the production of blastospores in a scale-up conditions. Specific optimization of salt and culture parameters in the scale up conditions and field study would be essential works to be followed.

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来源期刊
Mycobiology
Mycobiology AGRONOMYMYCOLOGY-MYCOLOGY
CiteScore
3.90
自引率
5.30%
发文量
41
审稿时长
22 weeks
期刊介绍: Mycobiology is an international journal devoted to the publication of fundamental and applied investigations on all aspects of mycology and their traditional allies. It is published quarterly and is the official publication of the Korean Society of Mycology. Mycobiology publishes reports of basic research on fungi and fungus-like organisms, including yeasts, filamentous fungi, lichen fungi, oomycetes, moulds, and mushroom. Topics also include molecular and cellular biology, biochemistry, metabolism, developmental biology, environmental mycology, evolution, ecology, taxonomy and systematics, genetics/genomics, fungal pathogen and disease control, physiology, and industrial biotechnology using fungi.
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