Mycobiology最新文献_第3页

Two New Species of Collybiopsis (Omphalotaceae, Agaricales) from Southwestern Guizhou, China. 文章标题贵州西南部大腹虫属二新种（鞘翅目，鞘翅目）。

IF 2.5 4区生物学

Mycobiology Pub Date : 2026-05-22 eCollection Date: 2026-01-01 DOI: 10.1080/12298093.2026.2668165

Heng-Ting Chen, Zhu Guo-Sheng, Yang Tong-Jing, Li Biao, Gong Guang-Lu, Huang Wang-Bing, Shang Nian-Jie, Gui Yang

引用次数: 0

Performance of FTIR-Based IR Biotyper for Identification and Strain Discrimination of Cryptococcus Species. 基于ftir的隐球菌IR生物分型及菌株鉴别性能研究。

IF 2.5 4区生物学

Mycobiology Pub Date : 2026-05-19 eCollection Date: 2026-01-01 DOI: 10.1080/12298093.2026.2671533

Eun Jeong Won, Yukyung Jeon, Tae Yeul Kim, Yong-Sun Bahn, Heungsup Sung, Mi-Na Kim

{"title":"Performance of FTIR-Based IR Biotyper for Identification and Strain Discrimination of Cryptococcus Species.","authors":"Eun Jeong Won, Yukyung Jeon, Tae Yeul Kim, Yong-Sun Bahn, Heungsup Sung, Mi-Na Kim","doi":"10.1080/12298093.2026.2671533","DOIUrl":"10.1080/12298093.2026.2671533","url":null,"abstract":"The IR Biotyper is a commercialized real-time typing tool based on a Fourier-transform infrared spectroscopy system. To date, it has not been evaluated for use with pathogenic Cryptococcus species complexes. This study aimed to assess the utility of the IR Biotyper for strain typing of pathogenic Cryptococcus species isolated from both environmental and clinical sources. We analyzed 51 isolates of Cryptococcus species, including clinical samples from two university hospitals, environmental isolates, and reference strains. Using a modified protocol optimized for Cryptococcus, we generated a total of 765 spectra that demonstrated high intra-isolate consistency. When the spectrum of Cryptococcus neoformans isolates was analyzed, distinct clusters were formed, clearly separating them from Cryptococcus species other than C. neoformans (Cryptococcus gattii, Cryptococcus deuterogattii, and Cryptococcus deneoformans). However, spectra from C. deuterogattii and C. gattii overlapped, indicating their close relatedness. Spectral discrimination was not clear between environmental and clinical isolates of C. neoformans. A dendrogram analysis grouped the 51 isolates into 10 clusters at a cutoff value of 6.245, which could discriminate four sequence types of C. neoformans. The IR Biotyper is useful for the identification and strain-level discrimination of C. neoformans, but its resolution is insufficient for reliable discrimination within the C. gattii complex. This initial study demonstrates further validation with larger, more diverse training sets is promising.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 3","pages":"319-325"},"PeriodicalIF":2.5,"publicationDate":"2026-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13188572/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147988154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Four Unrecorded Species of Trichoderma (Hypocreales, Ascomycota) from Forest Soils in Korea Based on Morphological and Phylogenetic Analyses. 韩国森林土壤中四种未记录的木霉（下creales， Ascomycota）的形态和系统发育分析。

IF 2.5 4区生物学

Mycobiology Pub Date : 2026-04-28 eCollection Date: 2026-01-01 DOI: 10.1080/12298093.2026.2658373

Minseo Cho, Seokyoon Jang, Young Min Lee, Changmu Kim, Sun Lul Kwon, Jae-Jin Kim

{"title":"Four Unrecorded Species of Trichoderma (Hypocreales, Ascomycota) from Forest Soils in Korea Based on Morphological and Phylogenetic Analyses.","authors":"Minseo Cho, Seokyoon Jang, Young Min Lee, Changmu Kim, Sun Lul Kwon, Jae-Jin Kim","doi":"10.1080/12298093.2026.2658373","DOIUrl":"10.1080/12298093.2026.2658373","url":null,"abstract":"Trichoderma is a diverse genus in the family Hypocreaceae (Hypocreales) with significant ecological roles in forest ecosystem. They contribute to nutrient cycling through decomposition of organic matter and enhance plant growth. Owing to this importance, the morphological, molecular, and enzymatic characteristics of Trichoderma species have been extensively studied worldwide. Approximately, 495 species have been reported worldwide, whereas only 50 species have been recorded in Korea. To narrow this gap, 11 Trichoderma strains were isolated from soil samples collected from fir and deciduous forests in 2013 and 2017. Morphological observations and multi-marker phylogenetic analyses were subsequently conducted using the second largest subunit of RNA polymerase II (rpb2) and translation elongation factor 1-alpha (tef1) regions. Among the strains, four unrecorded Trichoderma species in Korea were identified: T. chlamydosporum, T. concentricum, T. hirsutum, and T. italicum. This study expands the known diversity of Trichoderma in Korea and provides detailed cultural characteristics and phylogenetic relationships of these four unrecorded species.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 2","pages":"279-292"},"PeriodicalIF":2.5,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13126943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147817651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Loop-Mediated Isothermal Amplification (LAMP) Methods for Rapid Identification of Nephrotoxic Amanita Species. 环介导等温扩增（LAMP）快速鉴定肾毒性毒金刚伞的方法建立。

IF 2.5 4区生物学

Mycobiology Pub Date : 2026-04-28 eCollection Date: 2026-01-01 DOI: 10.1080/12298093.2026.2657154

Mengmeng Lai, Pengping Duan, Zhengmi He, Ping Zhang, Zuohong Chen

{"title":"Development of Loop-Mediated Isothermal Amplification (LAMP) Methods for Rapid Identification of Nephrotoxic Amanita Species.","authors":"Mengmeng Lai, Pengping Duan, Zhengmi He, Ping Zhang, Zuohong Chen","doi":"10.1080/12298093.2026.2657154","DOIUrl":"10.1080/12298093.2026.2657154","url":null,"abstract":"Mushroom poisoning remains the leading cause of foodborne morbidity and mortality in China. The mushrooms that cause acute kidney injury (AKI) belong to the species of Amanita genus section Roanokenses. These species exhibit highly similar morphological characteristics, which is a challenge for their identification for non-specialists. Although conventional DNA barcoding identification is reliable, it is time-consuming for rapid diagnosis. In this study, we developed a loop-mediated isothermal amplification (LAMP) method based on the internal transcribed spacer (ITS) region to enable rapid identification of five species within A. sect. Roanokenses which can cause AKI. The optimized LAMP system was rigorously evaluated for specificity and sensitivity, with comparative analysis performed against conventional PCR. The results demonstrated that the established LAMP method achieved visual detection without specialized equipment, exhibiting high specificity and a detection limit of 10 pg per reaction. Critically, this approach can reduce identification time to approximately 1 h and provides sensitivity 100 times greater than that of the conventional PCR protocol.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 2","pages":"293-302"},"PeriodicalIF":2.5,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13126936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147817664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rediscovery of Saprolegniales in the Philippines Reveals New Phylogenetic and Ecological Insights. 在菲律宾重新发现的Saprolegniales揭示了新的系统发育和生态学见解。

IF 2.5 4区生物学

Mycobiology Pub Date : 2026-04-18 eCollection Date: 2026-01-01 DOI: 10.1080/12298093.2026.2649504

Annrhe H Siscar, Reuel M Bennett

{"title":"Rediscovery of Saprolegniales in the Philippines Reveals New Phylogenetic and Ecological Insights.","authors":"Annrhe H Siscar, Reuel M Bennett","doi":"10.1080/12298093.2026.2649504","DOIUrl":"10.1080/12298093.2026.2649504","url":null,"abstract":"Saprolegniales (Phylum Oomycota), a group of fungus-like oomycetes, include economically important opportunistic pathogens responsible for disease outbreaks in aquatic organisms, yet their occurrence in tropical regions remains poorly documented. In this manuscript, five species of Saprolegniales were identified using an integrative taxonomic approach combining morphological characterization and phylogenetic analyses of nuclear (ITS, LSU) and mitochondrial (cox1, cox2) gene markers. Here we report the first Philippine records of Achlya glomerata and Saprolegnia ferax, and the second records of Achlya bisexualis, Achlya klebsiana, and Achlya prolifera. All species were isolated from both natural and artificial aquatic habitats, indicating broad ecological occurrence and potential relevance to aquatic health. Growth assays conducted at 15-35 °C demonstrated contrasting thermal responses, i.e., Achlya species exhibited enhanced growth with increasing temperature, whereas S. ferax showed reduced growth at warmer conditions. These patterns suggest that warm lowland aquaculture systems may be more vulnerable to Achlya-associated infections, while cooler highland environments may favor potential Saprolegnia-related outbreaks. Further, this study provides the first DNA sequence records of Philippine Saprolegniales, expanding molecular reference data and improving understanding of their diversity and distribution in tropical ecosystems.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 2","pages":"253-269"},"PeriodicalIF":2.5,"publicationDate":"2026-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13094230/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Querciphoma Phyllanthi-Emblicae, a Novel Leaf Endophyte in Amla (Phyllanthus Emblica L.) from Yunnan, China. 云南榆木属（Phyllanthus Emblica L.）一种新的叶内生植物——榆木栎属。

IF 2.5 4区生物学

Mycobiology Pub Date : 2026-04-02 eCollection Date: 2026-01-01 DOI: 10.1080/12298093.2026.2632471

Hongbo Jiang, Chengjie Li, Sheng Xu, Jayarama Darbhe Bhat, Turki M Dawoud, Laith Khalil Tawfeeq Al-Ani, Jianchu Xu, Rungtiwa Phookamsak

{"title":"Querciphoma Phyllanthi-Emblicae, a Novel Leaf Endophyte in Amla (Phyllanthus Emblica L.) from Yunnan, China.","authors":"Hongbo Jiang, Chengjie Li, Sheng Xu, Jayarama Darbhe Bhat, Turki M Dawoud, Laith Khalil Tawfeeq Al-Ani, Jianchu Xu, Rungtiwa Phookamsak","doi":"10.1080/12298093.2026.2632471","DOIUrl":"10.1080/12298093.2026.2632471","url":null,"abstract":"During our ongoing study of fungal endophytes associated with economic plants in Yunnan, China, Querciphoma phyllanthi-emblicae sp. nov. is introduced and illustrated. The new species was isolated from healthy leaves of Phyllanthus emblica (amla or Indian gooseberry) in Honghe County. Morphologically, the new species is typical of Querciphoma in having eustromatic, uni- to multi-loculate, setose conidiomata and ellipsoidal, hyaline, aseptate conidia. However, the new taxon can be distinguished from other Querciphoma species by producing long-necked pycnidia that are smaller than other Querciphoma species. Multigene phylogenetic analyses of a concatenated ITS, LSU, SSU, and tef1-α sequence matrix, based on maximum-likelihood and Bayesian inference criteria demonstrated that Q. phyllanthi-emblicae is a sister species to Q. foliicola in Leptosphaeriaceae. This is the first report of a fungal endophyte in Querciphoma occurring on Phyllanthus emblica. In addition, a synopsis of morphological features and geographic distributions of all Querciphoma species is provided in this article.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 2","pages":"217-225"},"PeriodicalIF":2.5,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13047844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147623472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of a bZIP Transcription Factor ZipD in Aspergillus flavus. 黄曲霉bZIP转录因子ZipD的研究。

IF 2 4区生物学

Mycobiology Pub Date : 2026-02-02 eCollection Date: 2026-01-01 DOI: 10.1080/12298093.2026.2622828

Da-Eun Jeong, He-Jin Cho, Hee-Soo Park

{"title":"Characterization of a bZIP Transcription Factor ZipD in Aspergillus flavus.","authors":"Da-Eun Jeong, He-Jin Cho, Hee-Soo Park","doi":"10.1080/12298093.2026.2622828","DOIUrl":"10.1080/12298093.2026.2622828","url":null,"abstract":"Aspergillus flavus produces aflatoxin B1, a Group 1 carcinogen that causes severe economic and agricultural losses and can lead to invasive aspergillosis in humans. During its growth, A. flavus develops hyphae that form a continuous mycelium, which is associated with host colonization and toxin production. Transcription factors are considered regulators of fungal development and pathogenicity. Among these families, bZIP proteins are associated with growth, metabolism, and stress tolerance. In A. fumigatus, the bZIP transcription factor ZipD regulates calcium signaling pathways and cell wall integrity. However, the ortholog of zipD in A. flavus has not been characterized. To address this gap, we generated a deletion mutant and a complemented strain of zipD in A. flavus. The zipD deletion mutant showed reduced colony growth and conidia production, but increased sclerotia formation. The ΔzipD strain exhibited hypersensitivity to osmotic stress-related agents, cell wall stress-related agents, and high calcium concentrations. Additionally, ΔzipD showed reduced conidial colonization in pathogenicity assays using corn, soybean, and soybean brick compared to the wild-type strain. All of these phenotypes were restored in the complemented strain. These results suggest that zipD functions as a regulator of growth, developmental processes, and pathogenic traits in A. flavus.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 1","pages":"208-216"},"PeriodicalIF":2.0,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12865826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146119349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revealing the Hidden Diversity of Aquatic and Terrestrial Fungi in Korea: 17 Newly Recorded Species. 揭示韩国水生和陆生真菌的隐藏多样性：17个新记录物种。

IF 2 4区生物学

Mycobiology Pub Date : 2026-01-22 eCollection Date: 2026-01-01 DOI: 10.1080/12298093.2026.2612803

Thuong T T Nguyen, Do Young Song, Su Jin Kim, Beom Seok Oh, Min Kyung Kim, Ki Hyun Kang, Young Jae Cho, Hye Won Yu, Sun Jeong Jeon, Dong Hee Kim, Hye Jin Hwang, Chang Soo Lee, Hye Yeon Mun, Hyang Burm Lee

{"title":"Revealing the Hidden Diversity of Aquatic and Terrestrial Fungi in Korea: 17 Newly Recorded Species.","authors":"Thuong T T Nguyen, Do Young Song, Su Jin Kim, Beom Seok Oh, Min Kyung Kim, Ki Hyun Kang, Young Jae Cho, Hye Won Yu, Sun Jeong Jeon, Dong Hee Kim, Hye Jin Hwang, Chang Soo Lee, Hye Yeon Mun, Hyang Burm Lee","doi":"10.1080/12298093.2026.2612803","DOIUrl":"10.1080/12298093.2026.2612803","url":null,"abstract":"This paper documents 17 newly recorded species belonging to the orders Eurotiales, Hypocreales, Pleosporales, Venturiales, and Wallemiales. These species were obtained from freshwater, brackish water, and soil habitats and identified using phenotypic techniques combined with DNA sequence data. Two species belonged to the order Eurotiales (Talaromyces calidominioluteus, and T. endophyticus); two species belonged to the order Hypocreales (Parasarocladium wereldwijsianum, and Simplicillium lamellicola); 11 species belonged to the order Pleosporales (Allophoma tropica, Alternaria longxiensis, Anteaglonium parvulum, Camarosporomyces flavigenus, Edenia achyranthi, Hongkongmyces thailandicus, Periconia imperatae, Pseudopithomyces pavgii, Septoriella huberti, Spegazzinia tessarthra, and Torula chiangmaiensis); one species belonged to the order Venturiales (Scolecobasidium terrestre); and one species belonged to the order Wallemiales (Wallemia mellicola). Detailed descriptions, illustrations, and phylogenetic analysis results of these species have been provided.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 1","pages":"178-207"},"PeriodicalIF":2.0,"publicationDate":"2026-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12829420/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146053214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nine Unrecorded Species of Phanerochaete (Polyporales, Basidiomycota) in Korea. 标题韩国原毛纲九种未记录种（多孢子目，担子菌科）。

IF 2 4区生物学

Mycobiology Pub Date : 2026-01-19 eCollection Date: 2026-01-01 DOI: 10.1080/12298093.2025.2610049

Minseo Cho, Yeonjae Yoo, Sang Hyun Lee, Dae Young Kwon, Hyeongsub Shin, Young Min Lee, Changmu Kim, Young Woon Lim, Sun Lul Kwon, Jae-Jin Kim

{"title":"Nine Unrecorded Species of Phanerochaete (Polyporales, Basidiomycota) in Korea.","authors":"Minseo Cho, Yeonjae Yoo, Sang Hyun Lee, Dae Young Kwon, Hyeongsub Shin, Young Min Lee, Changmu Kim, Young Woon Lim, Sun Lul Kwon, Jae-Jin Kim","doi":"10.1080/12298093.2025.2610049","DOIUrl":"10.1080/12298093.2025.2610049","url":null,"abstract":"Phanerochaete is one of the largest genera of the family Phanerochaetaceae within the order Polyporales, characterized by membranaceous basidiome and ellipsoid to cylindrical basidiospores. As white-rot saprotrophs, Phanerochaete species produce ligninolytic enzymes to degrade lignin and cellulosic materials. Several species have been applied to bioremediation, biopulping, and bioethanol industries due to their enzymatic abilities. While 123 Phanerochaete species have been reported globally, only 13 have been identified in Korea. To narrow this gap, we conducted a nationwide collection of specimens from 2008 to 2024. Subsequently, a comprehensive morphological and multi-marker phylogenetic analyses were performed using the nuclear ribosomal internal transcribed spacer (ITS) and nuclear large subunit ribosomal DNA (nLSU) regions. From 17 specimens, nine unrecorded Phanerochaete species were identified, namely P. albocremea, P. australis, P. cystidiata, P. fusca, P. parmastoi, P. porostereoides, P. rhizomorpha, P. subcarnosa, and P. taiwaniana. This study contributes to expand the known diversity of Phanerochaete in Korea, elucidating the morphological features and phylogenetic relationships of these previously unrecorded species.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 1","pages":"133-145"},"PeriodicalIF":2.0,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12818329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146019090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ergosterol Attenuates Palmitate-Induced Tenocyte Apoptosis Through the Suppression of ER Stress and Myocyte-Derived IL-15/METRNL-Mediated Crosstalk. 麦角甾醇通过抑制内质网应激和肌细胞来源的IL-15/ metrnl介导的串扰，减轻棕榈酸盐诱导的细胞凋亡。

IF 2 4区生物学

Mycobiology Pub Date : 2026-01-19 eCollection Date: 2026-01-01 DOI: 10.1080/12298093.2025.2612421

Min Kyung Pyo, Jun Hwi Ko, A M Abd El-Aty, Ji Hoon Jeong, Kyoung-Tae Lee, Tae Woo Jung

{"title":"Ergosterol Attenuates Palmitate-Induced Tenocyte Apoptosis Through the Suppression of ER Stress and Myocyte-Derived IL-15/METRNL-Mediated Crosstalk.","authors":"Min Kyung Pyo, Jun Hwi Ko, A M Abd El-Aty, Ji Hoon Jeong, Kyoung-Tae Lee, Tae Woo Jung","doi":"10.1080/12298093.2025.2612421","DOIUrl":"10.1080/12298093.2025.2612421","url":null,"abstract":"Ergosterol (EG), the main bioactive ingredient in Wolfiporia hoelen, has been shown to suppress inflammation and oxidative stress in various disease models. However, the effects of EG on endoplasmic reticulum (ER) stress and apoptosis in tenocytes in in vitro obesity models have not been studied. Protein expression in tenocytes was assessed by Western blotting. Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining was performed to distinguish apoptotic cells, and ELISA was conducted to quantify the protein content in the tenocyte culture medium. The autophagosomes were visualized as punctate structures via monodansylcadaverine (MDC) staining. In this study, we found that treatment with EG prevented apoptosis, collagen degradation, and ER stress and enhanced cell migration in tenocytes treated with palmitate. EG treatment augmented peroxisome proliferator-activated receptor delta (PPARδ) expression and the expression of autophagy markers. PPARδ small interfering RNA (siRNA) or 3-methyladenine, an autophagy inhibitor, abolished the effects of EG on palmitate-mediated tenocyte dysfunction. Interleukin 15 (IL-15) and meteorin-like protein (METRNL) from C2C12 myocytes treated with EG also ameliorated tenocyte injury caused by palmitate. Collectively, our findings suggest that EG exerts protective effects against palmitate-induced metabolic stress in tendinopathy by alleviating ER stress through the PPARδ/autophagy axis and enhancing IL-15- and METRNL-mediated muscle-tendon crosstalk, supporting its potential as a natural compound-based therapeutic candidate.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"54 1","pages":"167-177"},"PeriodicalIF":2.0,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12818288/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146018759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0