从韩国淡水环境中分离的四种未报道的青霉菌的分离和特性。

IF 2 4区 生物学 Q2 AGRONOMY
Mycobiology Pub Date : 2025-03-14 eCollection Date: 2025-01-01 DOI:10.1080/12298093.2025.2473141
Min-Gyu Kim, Seong-Keun Lim, Chang-Gi Back, Yoosun Oh, Wonsu Cheon, Hye Yeon Mun, Seung-Yeol Lee, Hee-Young Jung
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引用次数: 0

摘要

青霉菌属的真菌种类可以在各种不同的环境中找到。对青霉菌属的亚属分类进行了研究,并比较了其形态学和系统发育特征,将其划分为2个亚属、32个科和89个系。在这项研究中,从韩国的淡水环境、植物凋落物和附近的基质中分离出11株真菌菌株,并确定为以前未报道的物种。对内部转录间隔区(ITS)、β-微管蛋白(BenA)、钙调蛋白(CaM)和RNA聚合酶II亚基(RPB2)基因进行系统发育分析。然后利用连接的DNA序列构建邻居连接树,并将菌株与青霉属的近缘种进行比较。该菌株聚集在不同的系统发育谱系中,确定了它们的分类为P.污染型、P. jininfoshanicum、P. xuanhanense和P. soppii。NNIBRFG40229的分生孢子呈瓶状,是污染菌的特征;NNIBRFG1595为分生孢子,与P. jinfoshanicum一致;NNIBRFG5602呈绒毛状,有橙色色素沉着,类似于P. xuanhanense;NNIBRFG4602显示双轮生分生孢子,具圆柱形元,对应于soppii。本研究在国内首次报道了这些物种,增加了对它们的分类认识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation and Characterization of Four Unreported Penicillium Species Isolated from the Freshwater Environments in Korea.

The fungal species of the genus Penicillium can be found across a diverse array of environments. The infrageneric classification of the genus Penicillium has been studied with comparison of morphological and phylogenetical features, derived into two subgenus, 32 sections, and 89 series. In this study, 11 fungal strains were isolated from freshwater environments, plant litter, and nearby substrates in Korea and were identified as previously unreported species. The internal transcribed spacer (ITS) regions, β-tubulin (BenA), calmodulin (CaM), and RNA polymerase II subunit (RPB2) genes were analyzed for phylogenetic analyses. A neighbor-joining tree was then constructed using the concatenated DNA sequences, and the strains were compared with closely related species of the genus Penicillium. The strain clustered into distinct phylogenetic lineages, confirming their classification as P. contaminatum, P. jinfoshanicum, P. xuanhanense, and P. soppii. NNIBRFG40229 exhibits monoverticillate conidiophores with flask-shaped phialides, characteristic of P. contaminatum; NNIBRFG1595 presents divaricate conidiophores, consistent with P. jinfoshanicum; NNIBRFG5602 shows a velutinous texture with orange pigmentation, resembling P. xuanhanense; and NNIBRFG4602 shows biverticillate conidiophores with cylindrical metulae, corresponding to P. soppii. This study provides the first report of these species in Korea, enhancing taxonomic understanding.

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来源期刊
Mycobiology
Mycobiology AGRONOMYMYCOLOGY-MYCOLOGY
CiteScore
3.90
自引率
5.30%
发文量
41
审稿时长
22 weeks
期刊介绍: Mycobiology is an international journal devoted to the publication of fundamental and applied investigations on all aspects of mycology and their traditional allies. It is published quarterly and is the official publication of the Korean Society of Mycology. Mycobiology publishes reports of basic research on fungi and fungus-like organisms, including yeasts, filamentous fungi, lichen fungi, oomycetes, moulds, and mushroom. Topics also include molecular and cellular biology, biochemistry, metabolism, developmental biology, environmental mycology, evolution, ecology, taxonomy and systematics, genetics/genomics, fungal pathogen and disease control, physiology, and industrial biotechnology using fungi.
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