{"title":"从韩国淡水环境中分离的四种未报道的青霉菌的分离和特性。","authors":"Min-Gyu Kim, Seong-Keun Lim, Chang-Gi Back, Yoosun Oh, Wonsu Cheon, Hye Yeon Mun, Seung-Yeol Lee, Hee-Young Jung","doi":"10.1080/12298093.2025.2473141","DOIUrl":null,"url":null,"abstract":"<p><p>The fungal species of the genus <i>Penicillium</i> can be found across a diverse array of environments. The infrageneric classification of the genus <i>Penicillium</i> has been studied with comparison of morphological and phylogenetical features, derived into two subgenus, 32 sections, and 89 series. In this study, 11 fungal strains were isolated from freshwater environments, plant litter, and nearby substrates in Korea and were identified as previously unreported species. The internal transcribed spacer (ITS) regions, β-tubulin (<i>BenA</i>), calmodulin (<i>CaM</i>), and RNA polymerase II subunit (<i>RPB</i>2) genes were analyzed for phylogenetic analyses. A neighbor-joining tree was then constructed using the concatenated DNA sequences, and the strains were compared with closely related species of the genus <i>Penicillium</i>. The strain clustered into distinct phylogenetic lineages, confirming their classification as <i>P. contaminatum</i>, <i>P. jinfoshanicum</i>, <i>P. xuanhanense</i>, and <i>P. soppii</i>. NNIBRFG40229 exhibits monoverticillate conidiophores with flask-shaped phialides, characteristic of <i>P. contaminatum</i>; NNIBRFG1595 presents divaricate conidiophores, consistent with <i>P. jinfoshanicum</i>; NNIBRFG5602 shows a velutinous texture with orange pigmentation, resembling <i>P. xuanhanense</i>; and NNIBRFG4602 shows biverticillate conidiophores with cylindrical metulae, corresponding to <i>P. soppii</i>. This study provides the first report of these species in Korea, enhancing taxonomic understanding.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"53 3","pages":"269-279"},"PeriodicalIF":2.0000,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912253/pdf/","citationCount":"0","resultStr":"{\"title\":\"Isolation and Characterization of Four Unreported <i>Penicillium</i> Species Isolated from the Freshwater Environments in Korea.\",\"authors\":\"Min-Gyu Kim, Seong-Keun Lim, Chang-Gi Back, Yoosun Oh, Wonsu Cheon, Hye Yeon Mun, Seung-Yeol Lee, Hee-Young Jung\",\"doi\":\"10.1080/12298093.2025.2473141\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The fungal species of the genus <i>Penicillium</i> can be found across a diverse array of environments. The infrageneric classification of the genus <i>Penicillium</i> has been studied with comparison of morphological and phylogenetical features, derived into two subgenus, 32 sections, and 89 series. In this study, 11 fungal strains were isolated from freshwater environments, plant litter, and nearby substrates in Korea and were identified as previously unreported species. The internal transcribed spacer (ITS) regions, β-tubulin (<i>BenA</i>), calmodulin (<i>CaM</i>), and RNA polymerase II subunit (<i>RPB</i>2) genes were analyzed for phylogenetic analyses. A neighbor-joining tree was then constructed using the concatenated DNA sequences, and the strains were compared with closely related species of the genus <i>Penicillium</i>. The strain clustered into distinct phylogenetic lineages, confirming their classification as <i>P. contaminatum</i>, <i>P. jinfoshanicum</i>, <i>P. xuanhanense</i>, and <i>P. soppii</i>. NNIBRFG40229 exhibits monoverticillate conidiophores with flask-shaped phialides, characteristic of <i>P. contaminatum</i>; NNIBRFG1595 presents divaricate conidiophores, consistent with <i>P. jinfoshanicum</i>; NNIBRFG5602 shows a velutinous texture with orange pigmentation, resembling <i>P. xuanhanense</i>; and NNIBRFG4602 shows biverticillate conidiophores with cylindrical metulae, corresponding to <i>P. soppii</i>. This study provides the first report of these species in Korea, enhancing taxonomic understanding.</p>\",\"PeriodicalId\":18825,\"journal\":{\"name\":\"Mycobiology\",\"volume\":\"53 3\",\"pages\":\"269-279\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-03-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912253/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mycobiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/12298093.2025.2473141\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycobiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/12298093.2025.2473141","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
Isolation and Characterization of Four Unreported Penicillium Species Isolated from the Freshwater Environments in Korea.
The fungal species of the genus Penicillium can be found across a diverse array of environments. The infrageneric classification of the genus Penicillium has been studied with comparison of morphological and phylogenetical features, derived into two subgenus, 32 sections, and 89 series. In this study, 11 fungal strains were isolated from freshwater environments, plant litter, and nearby substrates in Korea and were identified as previously unreported species. The internal transcribed spacer (ITS) regions, β-tubulin (BenA), calmodulin (CaM), and RNA polymerase II subunit (RPB2) genes were analyzed for phylogenetic analyses. A neighbor-joining tree was then constructed using the concatenated DNA sequences, and the strains were compared with closely related species of the genus Penicillium. The strain clustered into distinct phylogenetic lineages, confirming their classification as P. contaminatum, P. jinfoshanicum, P. xuanhanense, and P. soppii. NNIBRFG40229 exhibits monoverticillate conidiophores with flask-shaped phialides, characteristic of P. contaminatum; NNIBRFG1595 presents divaricate conidiophores, consistent with P. jinfoshanicum; NNIBRFG5602 shows a velutinous texture with orange pigmentation, resembling P. xuanhanense; and NNIBRFG4602 shows biverticillate conidiophores with cylindrical metulae, corresponding to P. soppii. This study provides the first report of these species in Korea, enhancing taxonomic understanding.
期刊介绍:
Mycobiology is an international journal devoted to the publication of fundamental and applied investigations on all aspects of mycology and their traditional allies. It is published quarterly and is the official publication of the Korean Society of Mycology. Mycobiology publishes reports of basic research on fungi and fungus-like organisms, including yeasts, filamentous fungi, lichen fungi, oomycetes, moulds, and mushroom. Topics also include molecular and cellular biology, biochemistry, metabolism, developmental biology, environmental mycology, evolution, ecology, taxonomy and systematics, genetics/genomics, fungal pathogen and disease control, physiology, and industrial biotechnology using fungi.