Impact of Clove Oil on Biofilm Formation in Candida albicans and Its Effects on Mice with Candida Vaginitis.

IF 2 4区生物学 Q2 AGRONOMY

Mycobiology Pub Date : 2025-08-07 eCollection Date: 2025-01-01 DOI:10.1080/12298093.2025.2537477

Yuan Gao, Zhuo Wang, Xin Wang, Yan Xu, Jianghan Luo, Siming Li, Yang Yang, Lijun Yan, Xiang Zou

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引用次数: 0

Abstract

In this study, the effects of Clove oil (CEO) on biofilm formation of Candida albicans (C. albicans) were investigated using XTT and crystal violet staining methods. The therapeutic efficacy of CEO was studied using ICR mouse model with candida vaginitis. The potential targets and pathways of the CEO's effect against C. albicans were predicted by network pharmacology. The relative gene levels of CDC42, Ste11, Hst7, CEK1, and CPH1 were evaluated by qPCR. Subsequently, the protein levels of CDC42, CEK1, and p-CEK1 were determined by Western blot. The results indicated CEO can inhibit C. albicans adhesion, mycelium formation and biofilm maturation process. By regulating the related genes and proteins, the CEO can inhibit C. albicans biofilm maturation process, and weaken the ability of C. albicans to cause disease. For candida vaginitis ICR mouse model, CEO can lower inflammatory cytokines levels, alleviated the symptoms of vaginitis, and play a good therapeutic effect.

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丁香油对白色念珠菌生物膜形成的影响及其对小鼠念珠菌阴道炎的影响。

本研究采用XTT和结晶紫染色法研究丁香油（CEO）对白色念珠菌（C. albicans）生物膜形成的影响。采用ICR小鼠念珠菌性阴道炎模型，观察复方黄芪多糖对念珠菌性阴道炎的治疗效果。应用网络药理学方法预测了其抗白色念珠菌作用的潜在靶点和途径。采用qPCR检测CDC42、Ste11、Hst7、CEK1、CPH1基因的相对表达水平。Western blot检测CDC42、CEK1、p-CEK1蛋白水平。结果表明，CEO能抑制白色念珠菌粘附、菌丝形成和生物膜成熟过程。CEO通过调控相关基因和蛋白，抑制白色念珠菌生物膜成熟过程，削弱白色念珠菌致病能力。对于念珠菌阴道炎ICR小鼠模型，CEO能降低炎症因子水平，缓解阴道炎症状，起到良好的治疗效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Mycobiology AGRONOMYMYCOLOGY-MYCOLOGY

CiteScore

3.90

自引率

5.30%

发文量

审稿时长

22 weeks

期刊介绍： Mycobiology is an international journal devoted to the publication of fundamental and applied investigations on all aspects of mycology and their traditional allies. It is published quarterly and is the official publication of the Korean Society of Mycology. Mycobiology publishes reports of basic research on fungi and fungus-like organisms, including yeasts, filamentous fungi, lichen fungi, oomycetes, moulds, and mushroom. Topics also include molecular and cellular biology, biochemistry, metabolism, developmental biology, environmental mycology, evolution, ecology, taxonomy and systematics, genetics/genomics, fungal pathogen and disease control, physiology, and industrial biotechnology using fungi.