Yuan Gao, Zhuo Wang, Xin Wang, Yan Xu, Jianghan Luo, Siming Li, Yang Yang, Lijun Yan, Xiang Zou
{"title":"丁香油对白色念珠菌生物膜形成的影响及其对小鼠念珠菌阴道炎的影响。","authors":"Yuan Gao, Zhuo Wang, Xin Wang, Yan Xu, Jianghan Luo, Siming Li, Yang Yang, Lijun Yan, Xiang Zou","doi":"10.1080/12298093.2025.2537477","DOIUrl":null,"url":null,"abstract":"<p><p>In this study, the effects of Clove oil (CEO) on biofilm formation of <i>Candida albicans</i> (<i>C. albicans</i>) were investigated using XTT and crystal violet staining methods. The therapeutic efficacy of CEO was studied using ICR mouse model with candida vaginitis. The potential targets and pathways of the CEO's effect against <i>C. albicans</i> were predicted by network pharmacology. The relative gene levels of CDC42, Ste11, Hst7, CEK1, and CPH1 were evaluated by qPCR. Subsequently, the protein levels of CDC42, CEK1, and p-CEK1 were determined by Western blot. The results indicated CEO can inhibit <i>C. albicans</i> adhesion, mycelium formation and biofilm maturation process. By regulating the related genes and proteins, the CEO can inhibit <i>C. albicans</i> biofilm maturation process, and weaken the ability of <i>C. albicans</i> to cause disease. For candida vaginitis ICR mouse model, CEO can lower inflammatory cytokines levels, alleviated the symptoms of vaginitis, and play a good therapeutic effect.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"53 5","pages":"661-675"},"PeriodicalIF":2.0000,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12337725/pdf/","citationCount":"0","resultStr":"{\"title\":\"Impact of Clove Oil on Biofilm Formation in <i>Candida albicans</i> and Its Effects on Mice with Candida Vaginitis.\",\"authors\":\"Yuan Gao, Zhuo Wang, Xin Wang, Yan Xu, Jianghan Luo, Siming Li, Yang Yang, Lijun Yan, Xiang Zou\",\"doi\":\"10.1080/12298093.2025.2537477\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In this study, the effects of Clove oil (CEO) on biofilm formation of <i>Candida albicans</i> (<i>C. albicans</i>) were investigated using XTT and crystal violet staining methods. The therapeutic efficacy of CEO was studied using ICR mouse model with candida vaginitis. The potential targets and pathways of the CEO's effect against <i>C. albicans</i> were predicted by network pharmacology. The relative gene levels of CDC42, Ste11, Hst7, CEK1, and CPH1 were evaluated by qPCR. Subsequently, the protein levels of CDC42, CEK1, and p-CEK1 were determined by Western blot. The results indicated CEO can inhibit <i>C. albicans</i> adhesion, mycelium formation and biofilm maturation process. By regulating the related genes and proteins, the CEO can inhibit <i>C. albicans</i> biofilm maturation process, and weaken the ability of <i>C. albicans</i> to cause disease. For candida vaginitis ICR mouse model, CEO can lower inflammatory cytokines levels, alleviated the symptoms of vaginitis, and play a good therapeutic effect.</p>\",\"PeriodicalId\":18825,\"journal\":{\"name\":\"Mycobiology\",\"volume\":\"53 5\",\"pages\":\"661-675\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-08-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12337725/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mycobiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/12298093.2025.2537477\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycobiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/12298093.2025.2537477","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
Impact of Clove Oil on Biofilm Formation in Candida albicans and Its Effects on Mice with Candida Vaginitis.
In this study, the effects of Clove oil (CEO) on biofilm formation of Candida albicans (C. albicans) were investigated using XTT and crystal violet staining methods. The therapeutic efficacy of CEO was studied using ICR mouse model with candida vaginitis. The potential targets and pathways of the CEO's effect against C. albicans were predicted by network pharmacology. The relative gene levels of CDC42, Ste11, Hst7, CEK1, and CPH1 were evaluated by qPCR. Subsequently, the protein levels of CDC42, CEK1, and p-CEK1 were determined by Western blot. The results indicated CEO can inhibit C. albicans adhesion, mycelium formation and biofilm maturation process. By regulating the related genes and proteins, the CEO can inhibit C. albicans biofilm maturation process, and weaken the ability of C. albicans to cause disease. For candida vaginitis ICR mouse model, CEO can lower inflammatory cytokines levels, alleviated the symptoms of vaginitis, and play a good therapeutic effect.
期刊介绍：
Mycobiology is an international journal devoted to the publication of fundamental and applied investigations on all aspects of mycology and their traditional allies. It is published quarterly and is the official publication of the Korean Society of Mycology. Mycobiology publishes reports of basic research on fungi and fungus-like organisms, including yeasts, filamentous fungi, lichen fungi, oomycetes, moulds, and mushroom. Topics also include molecular and cellular biology, biochemistry, metabolism, developmental biology, environmental mycology, evolution, ecology, taxonomy and systematics, genetics/genomics, fungal pathogen and disease control, physiology, and industrial biotechnology using fungi.
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