Mycobiology最新文献

{"title":"Re-identification of Strains from <i>Aspergillus</i> Section <i>Aspergillus</i> and Description of Three Unrecorded Species from Korea.","authors":"Anbazhagan Mageswari, Daseul Lee, Le Dinh Thao, Donghun Kang, Dong-Hyun Kim, Seung-Beom Hong","doi":"10.1080/12298093.2024.2387952","DOIUrl":"10.1080/12298093.2024.2387952","url":null,"abstract":"<p><p>The section <i>Aspergillus</i> includes xerophilic fungi that are economically significant and broadly distributed in natural settings as well as human habitats and are recognized for their sustenance on substrates with low water activity. Accurate identification of fungal species is essential for any reliable advances in mycological research. In this study, 108 strains from the section <i>Aspergillus</i>, originating from Korea and conserved at the Korean Agricultural Culture Collection, were subjected to re-identification using a combined dataset that included partial sequences of β-tubulin (<i>BenA</i>), Calmodulin (<i>CaM</i>), and RNA polymerase II second largest subunit (<i>RPB2</i>) genes, along with their morphological characteristics. We confirmed the presence of 12 species among the 108 strains originally isolated from Korea. Of them, nine species have been formerly reported in Korea (<i>Aspergillus chevalieri</i>, <i>Aspergillus cibarius</i>, <i>Aspergillus cumulatus</i>, <i>Aspergillus glaucus</i>, <i>Aspergillus montevidensis</i>, <i>Aspergillus proliferans</i>, <i>Aspergillus pseudoglaucus</i>, <i>Aspergillus ruber</i>, and <i>Aspergillus tonophilus</i>), and 3 species (<i>Aspergillus aurantiacoflavus</i>, <i>Aspergillus intermedius</i>, and <i>Aspergillus niveoglaucus</i>) were found to be previously unreported to be isolated from Korea. Here, the detailed characteristic features of these three unexplored species are presented, including specific morphological traits, genetic variations, and ecological niches in Korea.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"52 5","pages":"267-277"},"PeriodicalIF":1.6,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11619013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Study of Mechanical and Biological Pretreatment for Releasing Spores of Black Truffle <i>Tuber aestivum</i>.","authors":"E V Malygina, N A Imidoeva, A Yu Belyshenko, M E Dmitrieva, V N Shelkovnikova, A A Vlasova, T Yu Telnova, M M Morgunova, A S Konovalov, D V Axenov-Gribanov","doi":"10.1080/12298093.2024.2391627","DOIUrl":"10.1080/12298093.2024.2391627","url":null,"abstract":"<p><p>It is well known that the number of true truffles in the wild is decreasing. The aim of the study was to develop an effective, simple and affordable method of asci disruption to release black truffle spores. It was shown that the spore release can be achieved by different ways, such as mechanical or biological destruction. Mechanical homogenization of fruiting bodies using an immersion blender in tandem with a ball mill was shown to be effective and led to destruction of at least 85% of asci and release of spores. Also, the first approach we applied was the biological method of spore activation performed by African and grape snails. As a result of digestion of truffle fruiting bodies, the spores not only lost their protective shells, but also changed their morphology, which promoted their germination <i>in vitro</i>. The spores obtained using these two methods are capable of forming mycelial hyphae on nutrient media. The results of our study can be used to prepare inoculum of <i>Tuber</i> spp. and to obtain their pure cultures in agriculture.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"52 5","pages":"278-286"},"PeriodicalIF":1.6,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11619011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetic and Morphological Analysis of a Novel Tolypocladium Species Isolated from Soil in South Korea","authors":"Gyeong-Mi Song, Leonid N. Ten, Seong-Keun Lim, Soo-Min Hong, Seung-Yeol Lee, Hee-Young Jung","doi":"10.1080/12298093.2024.2384776","DOIUrl":"https://doi.org/10.1080/12298093.2024.2384776","url":null,"abstract":"A fungal strain designated KNUF-22-014, belonging to the family Ophiocordycipitaceae, was isolated from a soil sample collected in Sobaeksan National Park, South Korea. Phylogenetic trees based on ...","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"93 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and Phylogenetic Characterization of Trichomerium koreanum sp. nov. and Exophiala radicis Belonging to Chaetothyriales Fungi in Korea","authors":"Seong-Keun Lim, Mohammad Hamizan Azmi, Jin-Sil Choi, Gwang-Jae Lim, Young-Kun Kim, Sang Jae Suh, Seung-Yeol Lee, Hee-Young Jung","doi":"10.1080/12298093.2024.2380553","DOIUrl":"https://doi.org/10.1080/12298093.2024.2380553","url":null,"abstract":"The order Chaetothyriales encompasses black yeasts and related fungi known for their diverse morphologies, asexual reproduction, and habitat preferences. In this study, we isolated two fungal strai...","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"67 14 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141883607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction.","authors":"","doi":"10.1080/12298093.2024.2384756","DOIUrl":"https://doi.org/10.1080/12298093.2024.2384756","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1080/12298093.2022.2038857.].</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"52 4","pages":"265-266"},"PeriodicalIF":1.6,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11494694/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Designing A Novel Primer Set for GAPDH Gene to Enhance Taxonomic and Phylogenetic Studies of Golovinomyces Species","authors":"Jun Hyuk Park, Young-Joon Choi","doi":"10.1080/12298093.2024.2379101","DOIUrl":"https://doi.org/10.1080/12298093.2024.2379101","url":null,"abstract":"Golovinomyces (Erysiphaceae, Ascomycota) is an obligate plant pathogenic group causing powdery mildew on diverse angiosperm plants, including economically significant crops. Despite advancements in...","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"20 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141741246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pythium aphanidermatum as a Novel Oomycete Pathogen Causing Leak on Potato (Solanum tuberosum) in Korea","authors":"Yu Jin Kim, Eui-Hwan Chung, Ki Deok Kim","doi":"10.1080/12298093.2024.2373506","DOIUrl":"https://doi.org/10.1080/12298093.2024.2373506","url":null,"abstract":"Potato (Solanum tuberosum L.) is one of the most important food crops in Korea. In July 2021 and 2022, dark black-rot symptoms with pink tinges were observed on field-grown potato tubers in Hongsun...","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"27 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141609689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Identification of a New Species of <i>Absidia</i> (Cunninghamellaceae, Mucorales) Isolated from Soil in Korea.","authors":"Seong-Keun Lim, Kallol Das, Gwang-Jae Lim, Jin-Sil Choi, Seung-Yeol Lee, Hee-Young Jung","doi":"10.1080/12298093.2024.2366047","DOIUrl":"10.1080/12298093.2024.2366047","url":null,"abstract":"<p><p>The species within the family Cunninghamellaceae during an investigation of soil microfungi in Korea, in which three strains were isolated from Gangwon, Chungbuk, and Gyeongbuk provinces, designated as KNUF-22-121A, KNUF-22-126A, and KNUF-22-316, respectively. Because the morphological and molecular analyses of these three strains were identical, KNUF-22-316 underwent further detailed study. Phylogenetic analyses based on the concatenated nucleotide sequences of the internal transcribed spacer region and the large subunit 28S rRNA gene revealed that the strain belonged to the genus <i>Absidia</i>, but occupied a distinct phylogenetic position. The strain KNUF-22-316 was compared with closely related species <i>Absidia radiata</i> CGMCC 3.16257^T and <i>Absidia yunnanensis</i> CGMCC 3.16259^T, morphologically different with shorter sporangiophores, smaller sporangia and columellae, and the consistent presence of collars. Here, we provide a detailed description and images of this proposed new species, which we have named <i>Absidia microsporangia</i> sp. nov.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"52 3","pages":"201-206"},"PeriodicalIF":1.6,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210407/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Eritadenine Production in Submerged Cultures of Shiitake (<i>Lentinula edodes</i> Berk. Pegler) Using Blue LED Light and Activated Charcoal. Revealing Eritadenine's Novel In Vitro Bioherbicidal Activity Against <i>Chrysanthemum morifolium</i>.","authors":"Byron Duran-Rivera, Felipe Rojas-Rodas, Wilber Silva López, Crhistian Gómez-Suárez, Dagoberto Castro Restrepo","doi":"10.1080/12298093.2024.2350207","DOIUrl":"10.1080/12298093.2024.2350207","url":null,"abstract":"<p><p>Eritadenine from shiitake mushroom is a secondary metabolite with hypocholesterolemic, hypotensive and antiparasitic properties, thus promising for pharmaceutical and agricultural applications. Eritadenine is obtained from submerged mycelial cultures of shiitake, but the actual yields remain unsatisfactory to explore potential applications or industrial-scale production. In this study, green and blue LED lights were tested to increase yields of eritadenine in submerged cultures of shiitake. Notably, blue LEDs increased yields by 13-14 times, reaching 165.7 mg/L, compared to darkness (11.2 mg/L) and green light (12.1 mg/L) (<i>p</i> < 0.05, Tukey test). Nitrogen sources yeast extract (YE) and peptone (at 2 g/L) increased eritadenine production. YE promoted 22.6 mg/L, while peptone 18.3 mg/L. The recovery of eritadenine was evaluated using amberlite and activated charcoal (AC) adsorption isotherms. AC demonstrated the highest adsorption rate, with 75 mg of eritadenine per gram of AC, according to the Freundlich isotherm. The desorption rate reached 93.95% at pH 10. The extract obtained from submerged cultures had eritadenine content of 63.31%, corresponding to 87.86% of recovery, according to HPLC analysis. Furthermore, the novel bioherbicidal potential of eritadenine was tested on <i>in vitro Chrysanthemum morifolium</i> plants. The cultures extract containing eritadenine had a detrimental impact on plant development, generating mortality of 100% at 3%, 0.5%, and 0.25%. Moreover, pure eritadenine exhibited a phytotoxic effect similar than glyphosate on leaves, stems and roots. These findings highlight the significant bioherbicidal properties of eritadenine. Further studies are needed to understand the biosynthetic pathway of eritadenine and its bioherbicidal properties on weeds and illicit crops.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"52 3","pages":"145-159"},"PeriodicalIF":1.6,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal Activity of 3-Hydrazinoquinoxaline-2-Thiol, a Novel Quinoxaline Derivative against Candida Species.","authors":"Abdelbagi Elfadil, Karem Ibrahem","doi":"10.1080/12298093.2024.2362497","DOIUrl":"10.1080/12298093.2024.2362497","url":null,"abstract":"<p><p><i>Candida</i> ranks as among the most frequently encountered fungal infections that associated with high morbidity and mortality. Quinoxaline derivatives are a group of small molecules that showed a promising antimicrobial activity. This study aimed to investigate the fungicidal effects of 3-hydrazinoquinoxaline-2-thiol against <i>Candida</i> in comparison with Amphotericin B <i>in vitro</i> as a reference. Also, we aim to assess the efficacy of 3-hydrazinoquinoxaline-2-thiol <i>in vivo</i> using mice oral candidiasis model. Fifty-six <i>Candida</i> isolates were subjected to susceptibility testing by broth microdilution method for 3-hydrazinoquinoxaline-2-thiol and Amphotericin B. Therefore, Minimal inhibitory concentrations (MIC) were assessed and compared. The oral candidiasis mice model was used to evaluate the activity of 3-hydrazinoquinoxaline-2-thiol <i>in vivo</i>. Microbiological evaluation of progression and ELISA were used in this study. 3-hydrazinoquinoxaline-2-thiol was more effective than Amphotericin B against most clinical isolates of <i>Candida albicans</i>. Higher effectiveness was seen against <i>Candida glabrata</i> and <i>Candida parapsilosis</i> isolates. However, the efficiency against <i>Candida tropicalis</i> isolates varies. 3-hydrazinoquinoxaline-2-thiol was also effective against <i>Pichia kudriavzevii</i> and <i>Clavispora lusitaniae</i>. 3-hydrazinoquinoxaline-2-thiol showed a good efficacy in mice model against <i>C. albicans</i> cells ATCC 10231. 3-hydrazinoquinoxaline-2-thiol has shown promising antifungal and anti-inflammatory activity against different <i>Candida</i> species. More tests and experiments are needed.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"52 3","pages":"191-200"},"PeriodicalIF":1.6,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}