利用农业废弃物生产聚酮清洁寄主的研究

IF 2 4区 生物学 Q2 AGRONOMY
Mycobiology Pub Date : 2025-02-13 eCollection Date: 2025-01-01 DOI:10.1080/12298093.2025.2460292
Hongbee Han, Rundong Liu, Jung-Jae Woo, Jae-Seoun Hur, Wonyong Kim
{"title":"利用农业废弃物生产聚酮清洁寄主的研究","authors":"Hongbee Han, Rundong Liu, Jung-Jae Woo, Jae-Seoun Hur, Wonyong Kim","doi":"10.1080/12298093.2025.2460292","DOIUrl":null,"url":null,"abstract":"<p><p>The plant pathogenic fungus <i>Ascochyta rabiei</i> produces solanapyrones, which are polyketide-derived secondary metabolites, during its saprobic growth on straws. Previously, we utilized <i>A. rabiei</i> as a heterologous host to produce a lichen-derived polyketide with anticancer activities. This study aimed to establish <i>A. rabiei</i> as a sustainable biorefinery for production of biologically active compounds through two objectives: optimizing culture conditions on agricultural waste substrates for polyketide production and generating a clean host using the Cre-loxP system for reusable antibiotic resistance markers. We found that soy hull is the most effective substrate among lignocellulosic materials. Neither light nor the addition of extra divalent cations were required for solanapyrone production in <i>A. rabiei</i>. Production of solanapyrones peaked 18 days after culture on soy hull pellets. To generate a clean host, we deleted the <i>pks1</i> gene, involved in melanin biosynthesis, and the <i>sol1</i> gene, responsible for solanapyrone production. Antibiotic resistance markers used in genetic transformation were recycled by establishing a Cre-loxP system in <i>A. rabiei</i>. In this system, Cre recombinase was expressed under the control of a promoter inducible during sporulation to mitigate the cytotoxicity of Cre. Here, we set the groundwork for developing <i>A. rabiei</i> as an environmentally-friendly biorefinery by generating a clean host with a Cre-loxP marker recycling system and optimizing growth conditions with soy hull pellets.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"53 2","pages":"225-235"},"PeriodicalIF":2.0000,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12010432/pdf/","citationCount":"0","resultStr":"{\"title\":\"Generation of a Clean Host for Polyketide Production Using Agricultural Wastes in <i>Ascochyta Rabiei</i>.\",\"authors\":\"Hongbee Han, Rundong Liu, Jung-Jae Woo, Jae-Seoun Hur, Wonyong Kim\",\"doi\":\"10.1080/12298093.2025.2460292\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The plant pathogenic fungus <i>Ascochyta rabiei</i> produces solanapyrones, which are polyketide-derived secondary metabolites, during its saprobic growth on straws. Previously, we utilized <i>A. rabiei</i> as a heterologous host to produce a lichen-derived polyketide with anticancer activities. This study aimed to establish <i>A. rabiei</i> as a sustainable biorefinery for production of biologically active compounds through two objectives: optimizing culture conditions on agricultural waste substrates for polyketide production and generating a clean host using the Cre-loxP system for reusable antibiotic resistance markers. We found that soy hull is the most effective substrate among lignocellulosic materials. Neither light nor the addition of extra divalent cations were required for solanapyrone production in <i>A. rabiei</i>. Production of solanapyrones peaked 18 days after culture on soy hull pellets. To generate a clean host, we deleted the <i>pks1</i> gene, involved in melanin biosynthesis, and the <i>sol1</i> gene, responsible for solanapyrone production. Antibiotic resistance markers used in genetic transformation were recycled by establishing a Cre-loxP system in <i>A. rabiei</i>. In this system, Cre recombinase was expressed under the control of a promoter inducible during sporulation to mitigate the cytotoxicity of Cre. Here, we set the groundwork for developing <i>A. rabiei</i> as an environmentally-friendly biorefinery by generating a clean host with a Cre-loxP marker recycling system and optimizing growth conditions with soy hull pellets.</p>\",\"PeriodicalId\":18825,\"journal\":{\"name\":\"Mycobiology\",\"volume\":\"53 2\",\"pages\":\"225-235\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-02-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12010432/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mycobiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/12298093.2025.2460292\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycobiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/12298093.2025.2460292","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0

摘要

植物病原菌Ascochyta rabiei在秸秆上生长时产生一种聚酮衍生的次生代谢物——茄酮。在此之前,我们利用a . rabiei作为异源寄主制备了一种具有抗癌活性的地衣源性聚酮。本研究旨在通过优化农业废弃物底物的培养条件以生产聚酮,以及利用Cre-loxP系统生成可重复使用的抗生素耐药性标记物的清洁宿主两个目标,建立rabiei作为生产生物活性化合物的可持续生物精炼厂。我们发现大豆壳是木质纤维素材料中最有效的底物。不需要光照,也不需要添加额外的二价阳离子来产生龙葵酮。在大豆壳颗粒上培养18天后,solanapyrones的产量达到峰值。为了产生一个干净的宿主,我们删除了参与黑色素生物合成的pks1基因和负责产生茄酮的sol1基因。利用遗传转化中使用的抗生素耐药标记,建立了rabiei的Cre-loxP系统。在该系统中,Cre重组酶在产孢诱导启动子的控制下表达,以减轻Cre的细胞毒性。本研究通过利用Cre-loxP标记回收系统生成清洁宿主,并利用大豆壳颗粒优化生长条件,为开发rabiei作为环境友好型生物精炼厂奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Generation of a Clean Host for Polyketide Production Using Agricultural Wastes in Ascochyta Rabiei.

The plant pathogenic fungus Ascochyta rabiei produces solanapyrones, which are polyketide-derived secondary metabolites, during its saprobic growth on straws. Previously, we utilized A. rabiei as a heterologous host to produce a lichen-derived polyketide with anticancer activities. This study aimed to establish A. rabiei as a sustainable biorefinery for production of biologically active compounds through two objectives: optimizing culture conditions on agricultural waste substrates for polyketide production and generating a clean host using the Cre-loxP system for reusable antibiotic resistance markers. We found that soy hull is the most effective substrate among lignocellulosic materials. Neither light nor the addition of extra divalent cations were required for solanapyrone production in A. rabiei. Production of solanapyrones peaked 18 days after culture on soy hull pellets. To generate a clean host, we deleted the pks1 gene, involved in melanin biosynthesis, and the sol1 gene, responsible for solanapyrone production. Antibiotic resistance markers used in genetic transformation were recycled by establishing a Cre-loxP system in A. rabiei. In this system, Cre recombinase was expressed under the control of a promoter inducible during sporulation to mitigate the cytotoxicity of Cre. Here, we set the groundwork for developing A. rabiei as an environmentally-friendly biorefinery by generating a clean host with a Cre-loxP marker recycling system and optimizing growth conditions with soy hull pellets.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Mycobiology
Mycobiology AGRONOMYMYCOLOGY-MYCOLOGY
CiteScore
3.90
自引率
5.30%
发文量
41
审稿时长
22 weeks
期刊介绍: Mycobiology is an international journal devoted to the publication of fundamental and applied investigations on all aspects of mycology and their traditional allies. It is published quarterly and is the official publication of the Korean Society of Mycology. Mycobiology publishes reports of basic research on fungi and fungus-like organisms, including yeasts, filamentous fungi, lichen fungi, oomycetes, moulds, and mushroom. Topics also include molecular and cellular biology, biochemistry, metabolism, developmental biology, environmental mycology, evolution, ecology, taxonomy and systematics, genetics/genomics, fungal pathogen and disease control, physiology, and industrial biotechnology using fungi.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信