Hongbee Han, Rundong Liu, Jung-Jae Woo, Jae-Seoun Hur, Wonyong Kim
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引用次数: 0
Abstract
The plant pathogenic fungus Ascochyta rabiei produces solanapyrones, which are polyketide-derived secondary metabolites, during its saprobic growth on straws. Previously, we utilized A. rabiei as a heterologous host to produce a lichen-derived polyketide with anticancer activities. This study aimed to establish A. rabiei as a sustainable biorefinery for production of biologically active compounds through two objectives: optimizing culture conditions on agricultural waste substrates for polyketide production and generating a clean host using the Cre-loxP system for reusable antibiotic resistance markers. We found that soy hull is the most effective substrate among lignocellulosic materials. Neither light nor the addition of extra divalent cations were required for solanapyrone production in A. rabiei. Production of solanapyrones peaked 18 days after culture on soy hull pellets. To generate a clean host, we deleted the pks1 gene, involved in melanin biosynthesis, and the sol1 gene, responsible for solanapyrone production. Antibiotic resistance markers used in genetic transformation were recycled by establishing a Cre-loxP system in A. rabiei. In this system, Cre recombinase was expressed under the control of a promoter inducible during sporulation to mitigate the cytotoxicity of Cre. Here, we set the groundwork for developing A. rabiei as an environmentally-friendly biorefinery by generating a clean host with a Cre-loxP marker recycling system and optimizing growth conditions with soy hull pellets.
期刊介绍:
Mycobiology is an international journal devoted to the publication of fundamental and applied investigations on all aspects of mycology and their traditional allies. It is published quarterly and is the official publication of the Korean Society of Mycology. Mycobiology publishes reports of basic research on fungi and fungus-like organisms, including yeasts, filamentous fungi, lichen fungi, oomycetes, moulds, and mushroom. Topics also include molecular and cellular biology, biochemistry, metabolism, developmental biology, environmental mycology, evolution, ecology, taxonomy and systematics, genetics/genomics, fungal pathogen and disease control, physiology, and industrial biotechnology using fungi.