Mycobiology最新文献_第7页

Comparative Analysis of Anticancer and Antibacterial Activities among Seven Trametes Species. 7种蕨类植物抗癌和抗菌活性的比较分析。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2247218

Ha Thi Kim Nguyen, Jiwon Lee, Yejin Park, Hyon Jin Park, Soon Kil Ahn, Jae Kwang Kim, Dong-Ku Kang, Minkyeong Kim, Chorong Ahn, Changmu Kim, Jaehyuk Choi

{"title":"Comparative Analysis of Anticancer and Antibacterial Activities among Seven Trametes Species.","authors":"Ha Thi Kim Nguyen, Jiwon Lee, Yejin Park, Hyon Jin Park, Soon Kil Ahn, Jae Kwang Kim, Dong-Ku Kang, Minkyeong Kim, Chorong Ahn, Changmu Kim, Jaehyuk Choi","doi":"10.1080/12298093.2023.2247218","DOIUrl":"https://doi.org/10.1080/12298093.2023.2247218","url":null,"abstract":"Species in the genus Trametes (Basidiomycota, Polyporales) have been used in natural medicine for a long time. Many studies reported that mycelia or fruiting bodies of Trametes spp. exhibited effects of antioxidant, anti-inflammatory, anticancer, and antimicrobial activities. However, comparative analysis in this genus is scarce due to limitation of morphological identification and the sample number. In this study, the 19 strains of seven Trametes species were chosen to generate a five-gene-based phylogeny with the 31 global references. In addition, 39 culture extracts were prepared for 13 strains to test for anticancer and antibacterial activities. Strong anticancer activities were found in several extracts from T. hirsuta and T. suaveolens. Anticancer activities of T. suaveolens, T. cf. junipericola and T. trogii were first described here. The antibacterial ability of T. versicolor and T. hirsuta extracts has been confirmed. The antibacterial activities of T. suaveolens have been reported at the first time in this study. These results suggest an efficient application of the genus Trametes as the drug resources especially for anticancer agents.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/64/48/TMYB_51_2247218.PMC10498812.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10268142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lecanora neobarkmaniana (Lecanorales, Lecanoraceae), A New Lichen Species from South Korea. 文章题目韩国地衣一新种(Lecanora neobarkmaniana, Lecanorales, Lecanoraceae)。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2168349

Jung Shin Park, Sang-Kuk Han, Soon-Ok Oh

引用次数: 0

Determination and Analysis of Hyper-Variable A Mating Types in Wild Strains of Lentinula edodes in Korea. 韩国香菇野生菌株超变量A交配型的测定与分析。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2022.2161157

Mi-Jeong Park, Eunjin Kim, Yeun Sug Jeong, Mi-Young Son, Yeongseon Jang, Kang-Hyeon Ka

{"title":"Determination and Analysis of Hyper-Variable A Mating Types in Wild Strains of Lentinula edodes in Korea.","authors":"Mi-Jeong Park, Eunjin Kim, Yeun Sug Jeong, Mi-Young Son, Yeongseon Jang, Kang-Hyeon Ka","doi":"10.1080/12298093.2022.2161157","DOIUrl":"https://doi.org/10.1080/12298093.2022.2161157","url":null,"abstract":"The diversity of A mating type in wild strains of Lentinula edodes was extensively analyzed to characterize and utilize them for developing new cultivars. One hundred twenty-three A mating type alleles, including 67 newly discovered alleles, were identified from 106 wild strains collected for the past four decades in Korea. Based on previous studies and current findings, a total of 130 A mating type alleles have been found, 124 of which were discovered from wild strains, indicating the hyper-variability of A mating type alleles of L. edodes. About half of the A mating type alleles in wild strains were found in more than two strains, whereas the other half of the alleles were found in only one strain. About 90% of A mating type combinations in dikaryotic wild strains showed a single occurrence. Geographically, diverse A mating type alleles were intensively located in the central region of the Korean peninsula, whereas only allele A17 was observed throughout Korea. We also found the conservation of the TCCCAC motif in addition to the previously reported motifs, including ATTGT, ACAAT, and GCGGAG, in the intergenic regions of A mating loci. Sequence comparison among some alleles indicated that accumulated mutation and recombination would contribute to the diversification of A mating type alleles in L. edodes. Our data support the rapid evolution of A mating locus in L. edodes, and would help to understand the characteristics of A mating loci of wild strains in Korea and help to utilize them for developing new cultivars.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/57/68/TMYB_51_2161157.PMC9946336.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9342401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro α-Amylase, α-Glucosidase, Pancreatic Lipase, Xanthine Oxidase Inhibiting Activity of Agaricus bisporus Extracts. 双孢蘑菇提取物体外抑制α-淀粉酶、α-葡萄糖苷酶、胰脂肪酶、黄嘌呤氧化酶活性的研究

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2176020

Jung Han Kim, Myoung Jun Jang, Youn Jin Park

{"title":"In Vitro α-Amylase, α-Glucosidase, Pancreatic Lipase, Xanthine Oxidase Inhibiting Activity of Agaricus bisporus Extracts.","authors":"Jung Han Kim, Myoung Jun Jang, Youn Jin Park","doi":"10.1080/12298093.2023.2176020","DOIUrl":"https://doi.org/10.1080/12298093.2023.2176020","url":null,"abstract":"In this study, the α-amylase inhibitory activity, α-glucosidase inhibitory activity, pancreatic lipase inhibitory activity, and Xanthine Oxidase inhibitory activity of the fruiting body extracts of 5 varieties of Agaricus bisporus (AB) were confirmed. First, the α-amylase inhibitory activity of AB12, AB13, AB18, AB34, and AB40 methanol extracts was lower than that of acarbose, a positive control, in all concentration ranges. The α-glucosidase inhibitory activity of the AB40, AB13, and AB12 methanol extracts at the extract concentration of 1.0 mg/mL was 80.5%, 81.3%, and 78.5%, respectively, similar to that of acarbose, a positive control. The pancreatic lipase inhibitory activity of the methanol extract of Agaricus bisporus fruiting body was significantly lower than that of the positive control orlistat in the concentration range of 50 ∼ 1.000 (mg/mL). The Xanthine Oxidase inhibitory activity was 0.5 ∼ 8.0 mg/mL of each extract, which was significantly lower than that of the positive control allopurinol in the same concentration range. However, the Xanthine Oxidase inhibitory activity of AB13 and AB40 at 8.0 mg/mL was about 70%, which was higher than that of other mushrooms. In conclusion, five kinds of Agaricus bisporus fruiting bodies seem to have inhibitory effects on enzymes such as α-amylase, α-glucosidase, pancreatic lipase, and Xanthine Oxidase that degrade starch and protein. In particular, it has an inhibitory effect and a reduction effect on xanthine oxidase that causes gout, so it is expected that it can be developed and used as a food or health supplement with health functional properties through future research.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e0/93/TMYB_51_2176020.PMC9946297.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9358051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolite Chemical Composition of the Bletilla striata (Thunb.) Reichb. f. Endophyte Penicillium oxalicum. 白芨(Bletilla striata)的代谢物化学成分Reichb。f.内生真菌:草青霉。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2216944

Ran Liu, Xuehua Han, Jing Gao, Min Luo, Dale Guo, Guangzhi Wang

{"title":"Metabolite Chemical Composition of the Bletilla striata (Thunb.) Reichb. f. Endophyte Penicillium oxalicum.","authors":"Ran Liu, Xuehua Han, Jing Gao, Min Luo, Dale Guo, Guangzhi Wang","doi":"10.1080/12298093.2023.2216944","DOIUrl":"https://doi.org/10.1080/12298093.2023.2216944","url":null,"abstract":"Penicillium oxalicum strain can be isolated from the Bletilla striata (Thunb.) Reichb. f. tubers. Its solid-state fermentation products are concentrated by percolation extraction. Separation and purification have been conducted to the ethyl acetate extracts by preparative HPLC. Based on the use of spectrometry, we have determined 17 known compounds, 12,13-dihydroxy-fumitremorgin C (1), pseurotin A (2), tyrosol (3), cyclo-(L-Pro-L-Val) (4), cis-4-hydroxy-8-O-methylmellein (5), uracil (6), cyclo-(L-Pro-L-Ala) (7), 1,2,3,4-tetrahydro-4-hydroxy-4-quinolin carboxylic acid (8), cyclo-(Gly-L-Pro) (9), 2'-deoxyuridine (10), 1-(β-D-ribofuranosyl)thymine (11), cyclo-(L-Val-Gly) (12), 2'-deoxythymidine (13), cyclo-(Gly-D-Phe) (14), cyclo-L-(4-hydroxyprolinyl)-D-leucine (15), cyclo-(L)-4-hydroxy-Pro-(L)-Phe (16), uridine (17). Here, we report compounds 1-3, 5, 7-8, 11-12, 14-17 are first found and isolated from this endophyte.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d6/0b/TMYB_51_2216944.PMC10288904.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9714404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Abiotic Factors on Fumosorinone Production from Cordyceps fumosorosea via Solid-State Fermentation. 非生物因素对烟孢虫草固态发酵产烟孢素酮的影响。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2216924

Tahir Khan, Dong-Hai Hou, Jin-Na Zhou, Yin-Long Yang, Hong Yu

{"title":"Effect of Abiotic Factors on Fumosorinone Production from Cordyceps fumosorosea via Solid-State Fermentation.","authors":"Tahir Khan, Dong-Hai Hou, Jin-Na Zhou, Yin-Long Yang, Hong Yu","doi":"10.1080/12298093.2023.2216924","DOIUrl":"https://doi.org/10.1080/12298093.2023.2216924","url":null,"abstract":"Cordyceps fumosorosea is an important species in the genus of Cordyceps, containing a variety of bioactive compounds, including fumosorinone (FU). This study was a ground-breaking assessment of FU levels in liquid and solid cultures. The present study focused on the impacts of solid-state fermentation (SSF) using solid substrates (wheat, oat, and rice), as well as the effects of fermentation parameters (pH, temperature, and incubation period), on the generation of FU. All the fermentation parameters had significant effects on the synthesis of FU. In a study of 25 °C, 5.5 pH, and 21 days of incubation period combinations calculated -to give maximal FU production, it was found that the optimal values were 25 °C, 5.5 pH, and 21 days, respectively. In a solid substrate medium culture, FU could be produced from SSF. At 30 days, a medium composed of rice yielded the most FU (798.50 mg/L), followed by a medium composed of wheat and oats (640.50 and 450.50 mg/L), respectively. An efficient method for increasing FU production on a large scale could be found in this approach. The results of this study might have multiple applications in different industrial fermentation processes.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0b/6e/TMYB_51_2216924.PMC10288913.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9714402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic Analysis of the Xanthoria elegans and Polyketide Synthase Gene Mining Based on the Whole Genome. 线虫黄原菌基因组分析及基于全基因组的聚酮合成酶基因挖掘。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2175428

Xiaolong Yuan, Yunqing Li, Ting Luo, Wei Bi, Jiaojun Yu, Yi Wang

{"title":"Genomic Analysis of the Xanthoria elegans and Polyketide Synthase Gene Mining Based on the Whole Genome.","authors":"Xiaolong Yuan, Yunqing Li, Ting Luo, Wei Bi, Jiaojun Yu, Yi Wang","doi":"10.1080/12298093.2023.2175428","DOIUrl":"https://doi.org/10.1080/12298093.2023.2175428","url":null,"abstract":"Xanthoria elegans is a lichen symbiosis, that inhabits extreme environments and can absorb UV-B. We reported the de novo sequencing and assembly of X. elegans genome. The whole genome was approximately 44.63 Mb, with a GC content of 40.69%. Genome assembly generated 207 scaffolds with an N50 length of 563,100 bp, N90 length of 122,672 bp. The genome comprised 9,581 genes, some encoded enzymes involved in the secondary metabolism such as terpene, polyketides. To further understand the UV-B absorbing and adaptability to extreme environments mechanisms of X. elegans, we searched the secondary metabolites genes and gene-cluster from the genome using genome-mining and bioinformatics analysis. The results revealed that 7 NR-PKSs, 12 HR-PKSs and 2 hybrid PKS-PKSs from X. elegans were isolated, they belong to Type I PKS (T1PKS) according to the domain architecture; phylogenetic analysis and BGCs comparison linked the putative products to two NR-PKSs and three HR-PKSs, the putative products of two NR-PKSs were emodin xanthrone (most likely parietin) and mycophelonic acid, the putative products of three HR-PKSs were soppilines, (+)-asperlin and macrolactone brefeldin A, respectively. 5 PKSs from X. elegans build a correlation between the SMs carbon skeleton and PKS genes based on the domain architecture, phylogenetic and BGC comparison. Although the function of 16 PKSs remains unclear, the findings emphasize that the genes from X. elegans represent an unexploited source of novel polyketide and utilization of lichen gene resources.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/09/8c/TMYB_51_2175428.PMC9946308.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9342404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erysiphe lonicerigena sp. nov., a Powdery Mildew Species Found on Lonicera harae. 金银花上发现的一种白粉病。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2182952

In-Young Choi, Lamiya Abasova, Joon-Ho Choi, Jung-Hee Park, Hyeon-Dong Shin

{"title":"Erysiphe lonicerigena sp. nov., a Powdery Mildew Species Found on Lonicera harae.","authors":"In-Young Choi, Lamiya Abasova, Joon-Ho Choi, Jung-Hee Park, Hyeon-Dong Shin","doi":"10.1080/12298093.2023.2182952","DOIUrl":"https://doi.org/10.1080/12298093.2023.2182952","url":null,"abstract":"A powdery mildew (Erysiphaceae) has been continuously collected on the leaves of Lonicera harae in the southern part of the Korean Peninsula, where this shrub is indigenous. Microscopic examination of the asexual morphs revealed that the current collections are differentiated from the all known Erysiphe species on Lonicera spp. by its longer conidiophores and longer conidia. Although the morphology of the chasmothecia is reminiscent of Erysiphe ehrenbergii and E. lonicerae, the specimens on L. harae differ from them in having smaller ascospores. A phylogenetic tree generated from a combined dataset of the internal transcribed spacer region and 28S rDNA gene sequences demonstrates that sequences obtained from three powdery mildew collections on L. harae clustered together as an independent species clade with high bootstrap values distant from other Erysiphe species on Lonicera, representing a species of its own. Based on morphological differences and molecular-phylogenetic results, the powdery mildew on L. harae is proposed as a new species, Erysiphe lonicerigena, and the holomorph of the fungus is described and illustrated in this study.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ab/70/TMYB_51_2182952.PMC10142395.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9397576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and Phylogenetic Analyses Reveal a New Species of Genus Monochaetia Belonging to the Family Sporocadaceae in Korea. 形态和系统发育分析揭示了韩国孢子科单菌属一新种。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2195735

Seong-Keun Lim, Kallol Das, Soo-Min Hong, Sang Jae Suh, Seung-Yeol Lee, Hee-Young Jung

{"title":"Morphological and Phylogenetic Analyses Reveal a New Species of Genus Monochaetia Belonging to the Family Sporocadaceae in Korea.","authors":"Seong-Keun Lim, Kallol Das, Soo-Min Hong, Sang Jae Suh, Seung-Yeol Lee, Hee-Young Jung","doi":"10.1080/12298093.2023.2195735","DOIUrl":"https://doi.org/10.1080/12298093.2023.2195735","url":null,"abstract":"The fungal strain belonging to the genus Monochaetia of the family Sporocadaceae was isolated from hairy long-horned toad beetle (Moechotypa diphysis) during the screening of microfungi associated with insects from Gangwon Province, Korea. The strain KNUF-6L2F produced white, light brown to dirty black surface, and olivaceous green colonies with the higher growth, while the closest strain M. ilicis KUMCC 15-0520T were light brown to brown, and M. schimae SAUCC 212201T light brown to brown toward center. The strain KNUF-6L2F produced shorter (5.7-14.0 μm) apical appendages than M. ilicis (6.0-24.0 μm), but similar to M. schimae (7.0-12.5 μm). Three median cells of KNUF-6L2F were light brown to olivaceous green, whereas brown and olivaceous cells were observed from M. ilicis and M. schimae, respectively. And the strain KNUF-6L2F produced larger conidiogenous cells than M. ilicis and M. schimae. Additionally, phylogenetic analyses based on molecular datasets of internal transcribed spacer (ITS) regions, translation elongation factor 1-alpha (TEF1α), and β-tubulin (TUB2) genes corroborated the strain's originality. Thus, the strain is different from other known Monochaetia species, according to molecular phylogeny and morophology, hence we suggested the new species Monochaetia mediana sp. nov. and provided a descriptive illustration.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/24/fa/TMYB_51_2195735.PMC10142316.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9397577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Transcriptome Analysis of Antrodia cinnamomea Mycelia from Different Wood Substrates. 不同木质基质樟芝菌丝体转录组分析。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2175434

Jiao-Jiao Chen, Zhang Zhang, Yi Wang, Xiao-Long Yuan, Juan Wang, Yu-Ming Yang, Yuan Zheng

{"title":"Transcriptome Analysis of Antrodia cinnamomea Mycelia from Different Wood Substrates.","authors":"Jiao-Jiao Chen, Zhang Zhang, Yi Wang, Xiao-Long Yuan, Juan Wang, Yu-Ming Yang, Yuan Zheng","doi":"10.1080/12298093.2023.2175434","DOIUrl":"https://doi.org/10.1080/12298093.2023.2175434","url":null,"abstract":"Antrodia cinnamomea, an edible and medicinal fungus with significant economic value and application prospects, is rich in terpenoids, benzenoids, lignans, polysaccharides, and benzoquinone, succinic and maleic derivatives. In this study, the transcriptome of A. cinnamomea cultured on the wood substrates of Cinnamomum glanduliferum (YZM), C. camphora (XZM), and C. kanehirae (NZM) was sequenced using the high-throughput sequencing technology Illumina HiSeq 2000, and the data were assembled by de novo strategy to obtain 78,729 Unigenes with an N50 of 4,463 bp. Compared with public databases, about 11,435, 6,947, and 5,994 Unigenes were annotated to the Non-Redundant (NR), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genome (KEGG), respectively. The comprehensive analysis of the mycelium terpene biosynthesis-related genes in A. cinnamomea revealed that the expression of acetyl-CoA acetyltransferase (AACT), acyl-CoA dehydrogenase (MCAD), 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA), mevalonate pyrophosphate decarboxylase (MVD), and isopentenyl diphosphate isomerase (IDI) was significantly higher on NZM compared to the other two wood substrates. Similarly, the expression of geranylgeranyltransferase (GGT) was significantly higher on YZM compared to NZM and XZM, and the expression of farnesyl transferase (FTase) was significantly higher on XZM. Furthermore, the expressions of 2,3-oxidized squalene cyclase (OCS), squalene synthase (SQS), and squalene epoxidase (SE) were significantly higher on NZM. Overall, this study provides a potential approach to explore the molecular regulation mechanism of terpenoid biosynthesis in A. cinnamomea.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ef/4f/TMYB_51_2175434.PMC9946338.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9358048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0