Mycobiology最新文献_第7页

Preussia jejuensis sp. nov., P. koreensis sp. nov., and P. isomera, Coprophilous Fungi Isolated from Horse Dung in Seopjikoji, Jeju Island in the Republic of Korea. 济州岛Seopjikoji马粪中分离的粪菌Preussia jejuensis sp. nov.、p.k reensis sp. nov.和p.r isomera .。

IF 1.6 4区生物学

Mycobiology Pub Date : 2025-02-07 eCollection Date: 2025-01-01 DOI: 10.1080/12298093.2025.2455214

HyeongJin Noh, Hyun Uk Cho, Seong Hwan Kim

{"title":"Preussia jejuensis sp. nov., P. koreensis sp. nov., and P. isomera, Coprophilous Fungi Isolated from Horse Dung in Seopjikoji, Jeju Island in the Republic of Korea.","authors":"HyeongJin Noh, Hyun Uk Cho, Seong Hwan Kim","doi":"10.1080/12298093.2025.2455214","DOIUrl":"10.1080/12298093.2025.2455214","url":null,"abstract":"The genus Preussia is widely distributed and includes species with ecological and biotechnological importance. In this study, morphological and phylogenetic analyses of the ITS and LSU rDNA sequences revealed two novel species, P. jejuensis sp. nov. and P. koreensis sp. nov., as well as one previously unrecorded species in Korea, P. isomera, from horse dung collected in Seopjikoji, Jeju Island, Republic of Korea. P. jejuensis sp. nov. is unique in producing conidia from conidiomata instead of teleomorphic structures, a feature not observed in any other known species of the Preussia genus. P. koreensis sp. nov. is morphologically distinguished by the absence of a neck, a smaller ascocarp diameter, smaller asci, and larger ascospores. In the phylogenetic analysis, P. jejuensis sp. nov. was closely related to P. isomera and S. minimoides, while P. koreensis sp. nov. was closely related to P. arizonica, P. persica, and S. minima. However, P. jejuensis sp. nov. and P. koreensis sp. nov. were clearly distinguished from their related species. This study expands the understanding of the biodiversity of coprophilous fungi and its distribution in Jeju Island, a region where horse breeding has been practiced for centuries, emphasizing the ecological importance of dung as a fungal habitat.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"53 2","pages":"200-213"},"PeriodicalIF":1.6,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Improved Sporulation Method for Producing Asexual Zoospores of Pythium aphanidermatum on Culture Medium. 一种在培养基上产生无性游动孢子的改进产孢方法。

IF 1.6 4区生物学

Mycobiology Pub Date : 2025-02-04 eCollection Date: 2025-01-01 DOI: 10.1080/12298093.2025.2452640

Yu Jin Kim, Seung Hyo Baek, Elena Volynchikova, Mee Kyung Sang, Ki Deok Kim

{"title":"An Improved Sporulation Method for Producing Asexual Zoospores of Pythium aphanidermatum on Culture Medium.","authors":"Yu Jin Kim, Seung Hyo Baek, Elena Volynchikova, Mee Kyung Sang, Ki Deok Kim","doi":"10.1080/12298093.2025.2452640","DOIUrl":"10.1080/12298093.2025.2452640","url":null,"abstract":"An improved sporulation method for Pythium aphanidermatum on 10% V8-juice agar was developed to generate substantial quantities of zoospores. We conducted experiments using a 4 × 4 factorial design to evaluate the effects of temperature (20, 25, 30, and 35 °C) and incubation time (2, 3, 4, and 5 days) on zoospore production from isolates HSv05 and KACC 48066. The results indicated that a temperature of 30 °C consistently yielded the highest numbers of zoospores across all incubation periods. Applying the same temperature (30 °C) and all incubation periods to other isolates (CCp03, CCp04, CCp05, HSv10, and KACC 40156) also resulted in higher numbers of zoospores regardless of incubation times compared with that under the control condition (25 °C and 2 days). Further virulence tests of isolate HSv05 revealed that high concentrations of zoospores (5 × 102 - 105 zoospores/ml) induced severe rot symptoms; however, a low concentration of 102 zoospores/ml produced only considerably weak symptoms on inoculated potato tubers. No symptoms appeared in tubers inoculated with 0 (uninoculated control) or 101 zoospores/ml. These findings suggest that our sporulation method can help obtain adequate zoospores for various basic and applied studies, including pathogen identification, virulence assessment, resistance resource screening, and control strategy development.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"53 1","pages":"120-127"},"PeriodicalIF":1.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11841104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Geoglossum subdifforme sp. nov. and G. simile, Two New Earth Tongues from South Korea. Geoglossum subdifforme sp.

IF 1.6 4区生物学

Mycobiology Pub Date : 2025-01-24 eCollection Date: 2025-01-01 DOI: 10.1080/12298093.2024.2440974

Chang Sun Kim, Young-Nam Kwag, Dae Ho Kim

引用次数: 0

Development of Genomic Simple Sequence Repeat Markers for Evaluating Resources of Armillaira ostoyae and Their Transferability to Armillaira gallica. 利用基因组简单序列重复标记评价蜜环菌资源及其在高卢蜜环菌中的可移植性。

IF 1.6 4区生物学

Mycobiology Pub Date : 2025-01-22 eCollection Date: 2025-01-01 DOI: 10.1080/12298093.2024.2444013

Sohee Kim, Hwayong Lee

{"title":"Development of Genomic Simple Sequence Repeat Markers for Evaluating Resources of Armillaira ostoyae and Their Transferability to Armillaira gallica.","authors":"Sohee Kim, Hwayong Lee","doi":"10.1080/12298093.2024.2444013","DOIUrl":"10.1080/12298093.2024.2444013","url":null,"abstract":"In this study, we aimed to develop simple sequence repeat (SSR) markers for evaluating resources in Armillaria ostoyae and examine their transferability to Armillaria gallica, related species. SSR markers were developed using the released A. ostoyae whole-genome sequence (GenBank assembly accession: GCA_900157425.1). The SSR regions were analyzed using the MISA (MIcroSAtellite identification tool) program. A total of 2319 SSR loci consisting of 922 (39.76%) mononucleotide, 763 (32.90%) trinucleotide, and 517 (22.29%) dinucleotide motifs were identified. Marker design involved an arbitrary choice of 150 SSR loci, considering motif abundance. A total of 22 strains of A. ostoyae were analyzed using the developed markers, and 105 markers were successfully amplified. The mean values of major allele frequency, number of alleles, expected heterozygosity, observed heterozygosity, and polymorphism information content (PIC) values were approximately 5.89, 5.4, 0.541, 0.255, and 0.504, respectively. A. gallica was analyzed, and 52 markers (49.5%) were successfully amplified to evaluate the transferability of the developed SSR markers. When these markers were used, the mean values of major allele frequency, number of alleles, expected heterozygosity, observed heterozygosity, and PIC were calculated to be approximately 0.615, 4.3, 0.517, 0.133, and 0.502, respectively. In conclusion, SSR markers were developed using the genome of A. ostoyae, and some of these markers exhibited transferability to A. gallica. These results can be used for resource evaluation of A. ostoyae and A. gallica.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"53 1","pages":"57-71"},"PeriodicalIF":1.6,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11780701/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eight Fungal Species Associated with Ambrosia Beetles in Korea. 与韩国香甲虫有关的八种真菌。

IF 1.6 4区生物学

Mycobiology Pub Date : 2025-01-16 eCollection Date: 2025-01-01 DOI: 10.1080/12298093.2024.2391629

Mi Jin Jeon, Sangwook Park, Jong Chul Jeong, Jongok Lim, Youngeun Han, Won-Jae Chi, Soonok Kim

引用次数: 0

Exploring Diversity within Chytridiales and Rhizophydiales (Chytridiomycota) in Korea. 韩国壶菌门和根菌门（壶菌门）多样性研究。

IF 1.6 4区生物学

Mycobiology Pub Date : 2025-01-16 eCollection Date: 2025-01-01 DOI: 10.1080/12298093.2024.2436204

Thuong T T Nguyen, Sun Jeong Jeon, Do Young Song, Dong Hee Kim, Hyang Burm Lee

引用次数: 0

Biocontrol of Fusarium oxysporum f. sp. cepae on Indonesian Local Garlic Plants (Lumbu Hijau) Using a Consortium of Bacillus amyloliquefaciens B1 and Arbuscular Mycorrhizal Fungi. 解淀粉芽孢杆菌B1与丛枝菌根真菌联合拮抗印尼大蒜尖孢镰刀菌

IF 1.6 4区生物学

Mycobiology Pub Date : 2025-01-16 eCollection Date: 2025-01-01 DOI: 10.1080/12298093.2024.2433826

Fibrianti Shinta Dewi, Rifani Rusiana Dewi, Abdul Latief Abadi, Adi Setiawan, Luqman Qurata Aini, Muhammad Akhid Syib'li

{"title":"Biocontrol of Fusarium oxysporum f. sp. cepae on Indonesian Local Garlic Plants (Lumbu Hijau) Using a Consortium of Bacillus amyloliquefaciens B1 and Arbuscular Mycorrhizal Fungi.","authors":"Fibrianti Shinta Dewi, Rifani Rusiana Dewi, Abdul Latief Abadi, Adi Setiawan, Luqman Qurata Aini, Muhammad Akhid Syib'li","doi":"10.1080/12298093.2024.2433826","DOIUrl":"10.1080/12298093.2024.2433826","url":null,"abstract":"Garlic (Allium sativum) is an indispensable ingredient for enriching and diversifying Indonesian cuisine taste. Indonesian people always use garlic for their daily dishes and any traditional foods. Due to its widespread culinary use, its availability in the market become critical. The main challenge to consistently growing this garlic is Fusarium oxysporum f. sp. cepae, which wilts Allium plants. The application of arbuscular mycorrhiza fungi (AMF) + Bacillus amyloliquefaciens B1 on local garlic varieties named Lumbu Hijau could effectively control F. oxysporum through in vitro and in vivo experiments. In the in vitro test, B. amyloliquefaciens B1 successfully suppressed the growth of F. oxysporum up to 53.41%. The consortium application in the greenhouse reduced disease incidence by up to 39.17%, and the efficacy of this biocontrol reached 84%. In addition, this approach also positively influenced plant growth, such as plant height, total wet shoot and root weight, and also tuber weight. As such, it is essential to use this consortium of microorganisms in field research and carry out a comprehensive investigation to identify any possible phenomena that may arise in the rhizosphere after application.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"53 1","pages":"18-26"},"PeriodicalIF":1.6,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11780700/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterologous Expression of Laccase1 from Cryphonectria parasitica in Saccharomyces cerevisiae. 酿酒酵母菌寄生漆膜酶1的异源表达。

IF 1.6 4区生物学

Mycobiology Pub Date : 2025-01-11 eCollection Date: 2025-01-01 DOI: 10.1080/12298093.2024.2439646

Kum-Kang So, Fatima Alejandra Hernandez Alvarado, Gui-Hwan Han, Jeong-Won Kim, Tae-Geum Kim, Dae-Hyuk Kim

{"title":"Heterologous Expression of Laccase1 from Cryphonectria parasitica in Saccharomyces cerevisiae.","authors":"Kum-Kang So, Fatima Alejandra Hernandez Alvarado, Gui-Hwan Han, Jeong-Won Kim, Tae-Geum Kim, Dae-Hyuk Kim","doi":"10.1080/12298093.2024.2439646","DOIUrl":"10.1080/12298093.2024.2439646","url":null,"abstract":"Laccases are enzymes capable of oxidizing phenolic compounds and are important tools in different industrial processes. Heterologous expression of laccases is of great interest in biotechnological applications but achieving high expression levels is challenging. Three different laccases have been identified in the chestnut blight fungus Cryphonectria parasitica, among which a tannic acid-inducible laccase (laccase3) was successfully expressed using Saccharomyces cerevisiae. To obtain high and stable expression of fungal laccases, we cloned the gene encoding an extracellular laccase (Laccase1) of C. parasitica into a yeast episomal vector, used the resulting vectors to transform S. cerevisiae, and optimized the culture conditions of the selected transformants for Laccase1 production. We also tested the significance of the signal peptide of Laccase1 in the secretion of expressed Laccase1 and compared it with the widely used rice amylase signal peptide. Among the four constructs tested using a yeast episomal vector, full-length Laccase1 containing an endogenous signal peptide, showed the highest laccase activity. Interestingly, the stability of the recombinant vector expressing laccase was lower than that of the mock transformant, suggesting a detrimental effect of the Laccase1-expressing vector on host cells. Thus, we optimized the culture conditions to produce Laccase1 and the resulting optimum culture conditions identified through one-factor-at-a -time (OFAT) were 2% sucrose; 3% yeast nitrogen base without amino acid; pH 5.0; and 30 °C. The laccase activity was found to be 2.2 U/mL in optimal culture conditions, resulting in a 6.5-fold increase compared to the conventional culture medium.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"53 1","pages":"36-46"},"PeriodicalIF":1.6,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11780702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endophytic Fungi Isolated from the Marine Macroalga Dictyopteris pacifica in Korea. 韩国太平洋巨藻内生真菌的分离。

IF 1.6 4区生物学

Mycobiology Pub Date : 2025-01-10 eCollection Date: 2025-01-01 DOI: 10.1080/12298093.2024.2440975

Ji-Won Kim, Yun-Jeong Kim, Ahn-Heum Eom

引用次数: 0

A New Species and Three New Records Belonging to Mucorales and Mortierellales from Korea. 标题韩国毛霉属和Mortierellales属一新种和三新记录。

IF 1.6 4区生物学

Mycobiology Pub Date : 2025-01-07 eCollection Date: 2024-01-01 DOI: 10.1080/12298093.2024.2434293

Thuong T T Nguyen, Ki Hyun Kang, Su Jin Kim, Min Kyung Kim, So Jeong Noh, Hyang Burm Lee

引用次数: 0