一种在培养基上产生无性游动孢子的改进产孢方法。

IF 2 4区 生物学 Q2 AGRONOMY
Mycobiology Pub Date : 2025-02-04 eCollection Date: 2025-01-01 DOI:10.1080/12298093.2025.2452640
Yu Jin Kim, Seung Hyo Baek, Elena Volynchikova, Mee Kyung Sang, Ki Deok Kim
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引用次数: 0

摘要

研究了一种在10% V8-juice琼脂培养基上对蛇皮霉(Pythium aphanidermatum)进行改良的产孢方法,可产生大量游动孢子。我们采用4 × 4因子设计来评估温度(20、25、30和35℃)和孵育时间(2、3、4和5天)对分离HSv05和KACC 48066虫孢子产生的影响。结果表明,在30°C的温度下,在所有孵育期均产生最高数量的游动孢子。与对照条件(25℃,2天)相比,对其他分离株(CCp03、CCp04、CCp05、HSv10和KACC 40156)施加相同的温度(30℃)和相同的孵育期,无论孵育时间如何,也能产生更多的动物孢子。进一步的毒力试验表明,高浓度的游动孢子(5 × 102 ~ 105个游动孢子/ml)可引起严重的腐病症状;然而,102个游动孢子/ml的低浓度对接种的马铃薯块茎只产生相当弱的症状。接种0(未接种对照)或101个游动孢子/ml的块茎未出现症状。这些发现表明,我们的产孢方法可以帮助获得足够的游动孢子,用于各种基础和应用研究,包括病原体鉴定、毒力评估、抗性资源筛选和控制策略制定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

An Improved Sporulation Method for Producing Asexual Zoospores of <i>Pythium aphanidermatum</i> on Culture Medium.

An Improved Sporulation Method for Producing Asexual Zoospores of <i>Pythium aphanidermatum</i> on Culture Medium.

An Improved Sporulation Method for Producing Asexual Zoospores of <i>Pythium aphanidermatum</i> on Culture Medium.

An Improved Sporulation Method for Producing Asexual Zoospores of Pythium aphanidermatum on Culture Medium.

An improved sporulation method for Pythium aphanidermatum on 10% V8-juice agar was developed to generate substantial quantities of zoospores. We conducted experiments using a 4 × 4 factorial design to evaluate the effects of temperature (20, 25, 30, and 35 °C) and incubation time (2, 3, 4, and 5 days) on zoospore production from isolates HSv05 and KACC 48066. The results indicated that a temperature of 30 °C consistently yielded the highest numbers of zoospores across all incubation periods. Applying the same temperature (30 °C) and all incubation periods to other isolates (CCp03, CCp04, CCp05, HSv10, and KACC 40156) also resulted in higher numbers of zoospores regardless of incubation times compared with that under the control condition (25 °C and 2 days). Further virulence tests of isolate HSv05 revealed that high concentrations of zoospores (5 × 102 - 105 zoospores/ml) induced severe rot symptoms; however, a low concentration of 102 zoospores/ml produced only considerably weak symptoms on inoculated potato tubers. No symptoms appeared in tubers inoculated with 0 (uninoculated control) or 101 zoospores/ml. These findings suggest that our sporulation method can help obtain adequate zoospores for various basic and applied studies, including pathogen identification, virulence assessment, resistance resource screening, and control strategy development.

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来源期刊
Mycobiology
Mycobiology AGRONOMYMYCOLOGY-MYCOLOGY
CiteScore
3.90
自引率
5.30%
发文量
41
审稿时长
22 weeks
期刊介绍: Mycobiology is an international journal devoted to the publication of fundamental and applied investigations on all aspects of mycology and their traditional allies. It is published quarterly and is the official publication of the Korean Society of Mycology. Mycobiology publishes reports of basic research on fungi and fungus-like organisms, including yeasts, filamentous fungi, lichen fungi, oomycetes, moulds, and mushroom. Topics also include molecular and cellular biology, biochemistry, metabolism, developmental biology, environmental mycology, evolution, ecology, taxonomy and systematics, genetics/genomics, fungal pathogen and disease control, physiology, and industrial biotechnology using fungi.
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