Mycobiology最新文献_第8页

Are Current Aspergillus sojae Strains Originated from a Native Aflatoxigenic Aspergillus Species Population Also Present in California? 目前的大豆曲霉菌株是否起源于当地的黄曲霉种群?

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2217495

Perng-Kuang Chang, Sui Sheng T Hua

{"title":"Are Current Aspergillus sojae Strains Originated from a Native Aflatoxigenic Aspergillus Species Population Also Present in California?","authors":"Perng-Kuang Chang, Sui Sheng T Hua","doi":"10.1080/12298093.2023.2217495","DOIUrl":"https://doi.org/10.1080/12298093.2023.2217495","url":null,"abstract":"Aspergillus sojae has long been considered a domesticated strain of Aspergillus parasiticus. This study delineated relationships among the two species and an Aspergillus PWE36 isolate. Of 25 examined clustered aflatoxin genes of PWE36, 20 gene sequences were identical to those of A. sojae, but all had variations to those of A. parasiticus. Additionally, PWE36 developmental genes of conidiation and sclerotial formation, overall, shared higher degrees of nucleotide sequence identity with A. sojae genes than with A. parasiticus genes. Examination of defective cyclopiazonic acid gene clusters revealed that the PWE36 deletion pattern was identical only to those of A. sojae. Using A. sojae SMF134 genome sequence as a reference, visualization of locally collinear blocks indicated that PWE36 shared higher genome sequence homologies with A. sojae than with A. parasiticus. Phylogenetic inference based on genome-wide single nucleotide polymorphisms (SNPs) and total SNP counts showed that A. sojae strains formed a monophyletic clade and were clonal. Two (Argentinian and Ugandan) A. parasiticus isolates but not including an Ethiopian isolate formed a monophyletic clade, which showed that A. parasiticus population is genetically diverse and distant to A. sojae. PWE36 and A. sojae shared a most recent common ancestor (MRCA). The estimated divergence time for PWE36 and A. sojae was about 0.4 mya. Unlike Aspergillus oryzae, another koji mold that includes genetically diverse populations, the findings that current A. sojae strains formed a monophyletic group and shared the MRCA with PWE36 allow A. sojae to be continuously treated as a species for food safety reasons.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10288891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9706143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Asymbiotic Spore Production of Rhizoglomus intraradices in a Medium Containing Myristate. 在含有肉豆肉酸盐的培养基中，根状芽孢杆菌的非共生孢子产生。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2216500

Jae-Eui Cha, Ahn-Heum Eom

引用次数: 0

A Novel Acremonium Species Isolated from Air Samples in Korea. 从韩国空气样本中分离的一种新的顶孢菌。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2242646

Jung-Min Lee, Jae-Eui Cha, Young-Sil Yoon, Ahn-Heum Eom

引用次数: 0

Isolation and Characterization of Xylanase from a Novel Strain, Penicillium menonorum SP10. menonorum青霉SP10木聚糖酶的分离与鉴定。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2247221

Thi Thu Huong Luong, Supattra Poeaim, Narumon Tangthirasunun

{"title":"Isolation and Characterization of Xylanase from a Novel Strain, Penicillium menonorum SP10.","authors":"Thi Thu Huong Luong, Supattra Poeaim, Narumon Tangthirasunun","doi":"10.1080/12298093.2023.2247221","DOIUrl":"https://doi.org/10.1080/12298093.2023.2247221","url":null,"abstract":"Xylanase has been applied in various sectors, such as biomass conversion, paper, pulp, textiles, and pharmaceutical industries. This study aimed to isolate and screen potential xylanase-producing fungi from the soil of Suphan Buri Province, Thailand. Fifteen fungi were isolated, and their xylanase activities were tested by the qualitative method. The result showed that isolate SP3, SP10 and SP15 gave high xylanase activity with potency index (PI) of 2.32, 2.01 and 1.82, respectively. These fungi were selected for the xylanase quantitative test, isolate SP10 performed the highest xylanase activity with 0.535 U/mL. Through molecular methods using the β-tubulin gene, isolate SP10 was identified as Penicillium menonorum. The xylanase characteristics from P. menonorum SP10 were determined, including the xylanase isoforms and the optimum pH and temperature. The xylanase isoforms on SDS-PAGE indicated that P. menonorum SP10 produced two xylanases (45 and 54 kDa). Moreover, its xylanase worked optimally at pH 6 and 55 °C while reaching 61% activity at 65 °C. These results proposed P. menonorum SP10 as a good candidate for industrial uses, especially in poultry feed and pulp industries, to improve yield and economic efficiency under slightly acidic and high-temperature conditions.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/73/64/TMYB_51_2247221.PMC10498789.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10261165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Development and Optimization of Culture Medium for the Production of Glabridin by Aspergillus eucalypticola: An Endophytic Fungus Isolated from Glycyrrhiza glabra L. (Fabaceae). 光甘草内生真菌桉叶曲霉生产光甘草定培养基的开发与优化。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2225253

Parisa Bahadori Ganjabadi, Mohsen Farzaneh, Mohammad Hossein Mirjalili

{"title":"Development and Optimization of Culture Medium for the Production of Glabridin by Aspergillus eucalypticola: An Endophytic Fungus Isolated from Glycyrrhiza glabra L. (Fabaceae).","authors":"Parisa Bahadori Ganjabadi, Mohsen Farzaneh, Mohammad Hossein Mirjalili","doi":"10.1080/12298093.2023.2225253","DOIUrl":"https://doi.org/10.1080/12298093.2023.2225253","url":null,"abstract":"Glabridin is a well-known active isoflavone found in the root of licorice (Glycyrrhiza glabra L.) that possess a wide range of biological activity. Plant cells, hairy roots, and fungal endophytes cultures are the most important alternative methods for plant resources conservation and sustainable production of natural compounds, which has received much attention in recent decades. In the present study, an efficient culture condition was optimized for the biomass accumulation and glabridin production from fungal endophyte Aspergillus eucalypticola SBU-11AE isolated from licorice root. Type of culture medium, range of pH, and licorice root extract (as an elicitor) were tested. The results showed that the highest and lowest biomass production was observed on PCB medium (6.43 ± 0.32 g/l) and peptone malt (5.85 + 0.11 g/l), respectively. The medium culture PCB was produced the highest level of glabridin (7.26 ± 0.44 mg/l), while the lowest level (4.47 ± 0.02 mg/l) was obtained from the medium peptone malt. The highest biomass (8.51 ± 0.43 g/l) and glabridin (8.30 ± 0.51 mg/l) production were observed from the PCB medium adjusted with pH = 6, while the lowest value of both traits was obtained from the same medium with pH = 7. The highest production of total glabridin (10.85 ± 0.84 mg/l) was also obtained from the culture medium treated with 100 mg/l of the plant root extract. This information can be interestingly used for the commercialization of glabridin production for further industrial applications.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/47/e8/TMYB_51_2225253.PMC10498790.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10261167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Wound Healing Potential of Lignosus rhinocerus and Other Ethno-myco Wound Healing Agents. 犀牛木与其他民族麦可伤口愈合剂的伤口愈合潜力。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2022.2164641

Hui-Yeng Y Yap, Mohammad Farhan Ariffeen Rosli, Soon-Hao Tan, Boon-Hong Kong, Shin-Yee Fung

{"title":"The Wound Healing Potential of Lignosus rhinocerus and Other Ethno-myco Wound Healing Agents.","authors":"Hui-Yeng Y Yap, Mohammad Farhan Ariffeen Rosli, Soon-Hao Tan, Boon-Hong Kong, Shin-Yee Fung","doi":"10.1080/12298093.2022.2164641","DOIUrl":"https://doi.org/10.1080/12298093.2022.2164641","url":null,"abstract":"Wound care has become increasingly important over the years. Various synthetic products for wound care treatment have been reported to cause toxic side effects and therefore natural products are in significant demand as they have minimal side effects. The presence of bioactive compounds in medicinal mushrooms contributes to various biological activities which assist in the early inflammatory phase, keratinocyte proliferation, and its migration enhancement which are pertinent to wound rehabilitation. Lignosus rhinocerus (tiger milk mushroom) can reduce the inflammation phase in wound healing by fighting off bacterial infection and modulating pro-inflammatory cytokines expression in the early stage to avoid prolonged inflammation and tissue damage. The antibacterial, immunomodulating, and anti-inflammatory activities exhibited by most macrofungi play a key role in enhancing wound healing. Several antibacterial and antifungal compounds sourced from traditional botanicals/products may prevent further complications and reoccurrence of injury to a wounded site. Scientific studies are actively underway to ascertain the potential use of macrofungi as a wound healing agent.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/df/54/TMYB_51_2164641.PMC9946334.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9342402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Current Studies on Bakanae Disease in Rice: Host Range, Molecular Identification, and Disease Management. 水稻Bakanae病研究进展:寄主范围、分子鉴定和病害防治

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2241247

Yu Na An, Chandrasekaran Murugesan, Hyowon Choi, Ki Deok Kim, Se-Chul Chun

{"title":"Current Studies on Bakanae Disease in Rice: Host Range, Molecular Identification, and Disease Management.","authors":"Yu Na An, Chandrasekaran Murugesan, Hyowon Choi, Ki Deok Kim, Se-Chul Chun","doi":"10.1080/12298093.2023.2241247","DOIUrl":"https://doi.org/10.1080/12298093.2023.2241247","url":null,"abstract":"The seed borne disease such as bakanae is difficult to control. Crop yield loss caused by bakanae depending on the regions and varieties grown, ranging from 3.0% to 95.4%. Bakanae is an important disease of rice worldwide and the pathogen was identified as Fusarium fujikuroi Nirenberg (teleomorph: Gibberella fujikuroi Sawada). Currently, four Fusaria (F. fujikuroi, F. proliferatum, F. verticillioides and F. andiyazi) belonging to F. fujikuroi species complex are generally known as the pathogens of bakanae. The infection occurs through both seed and soil-borne transmission. When infection occurs during the heading stage, rice seeds become contaminated. Molecular detection of pathogens of bakanae is important because identification based on morphological and biological characters could lead to incorrect species designation and time-consuming. Seed disinfection has been studied for a long time in Korea for the management of the bakanae disease of rice. As seed disinfectants have been studied to control bakanae, resistance studies to chemicals have been also conducted. Presently biological control and resistant varieties are not widely used. The detection of this pathogen is critical for seed certification and for preventing field infections. In South Korea, bakanae is designated as a regulated pathogen. To provide highly qualified rice seeds to farms, Korea Seed & Variety Service (KSVS) has been producing and distributing certified rice seeds for producing healthy rice in fields. Therefore, the objective of the study is to summarize the recent progress in molecular identification, fungicide resistance, and the management strategy of bakanae.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/59/63/TMYB_51_2241247.PMC10498795.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10268140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Identification of Fusarium Basal Rot Pathogens of Onion and Evaluation of Fungicides against the Pathogens. 洋葱基底腐病病原菌鉴定及杀菌剂评价。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2243759

Jong-Hwan Shin, Ha-Kyoung Lee, Chang-Gi Back, Soo-Hyun Kang, Ji-Won Han, Seong-Chan Lee, You-Kyoung Han

{"title":"Identification of Fusarium Basal Rot Pathogens of Onion and Evaluation of Fungicides against the Pathogens.","authors":"Jong-Hwan Shin, Ha-Kyoung Lee, Chang-Gi Back, Soo-Hyun Kang, Ji-Won Han, Seong-Chan Lee, You-Kyoung Han","doi":"10.1080/12298093.2023.2243759","DOIUrl":"https://doi.org/10.1080/12298093.2023.2243759","url":null,"abstract":"Onion (Allium cepa L.) is an economically important vegetable crop worldwide. However, various fungal diseases, including Fusarium basal rot (FBR), neck rot, and white rot, reduce onion production or bulb storage life. FBR caused by Fusarium species is among the most destructive onion diseases. In this study, we identified Fusarium species associated with FBR in Jeolla and Gyeongsang Provinces in South Korea and evaluated fungicides against the pathogens. Our morphological and molecular analyses showed that FBR in onions is associated with Fusarium commune, Fusarium oxysporum, and Fusarium proliferatum. We selected seven fungicides (fludioxonil, hexaconazole, mandestrobin, penthiopyrad, prochloraz-manganese, pydiflumetofen, and tebuconazole) and evaluated their inhibitory effects on mycelial growth of the pathogens at three different concentrations (0.01, 0.1, and 1 mg/mL). We found that prochloraz-manganese was highly effective, inhibiting 100% of the mycelial growth of the pathogens at all concentrations, followed by tebuconazole. Fludioxonil showed < 50% inhibition at 1 mg/mL for the tested isolates.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/59/87/TMYB_51_2243759.PMC10498799.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10268141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Development of a Transformation System for the Medicinal Fungus Sanghuangporus baumii and Acquisition of High-Value Strain. 药用真菌桑黄孢子菌转化体系的建立及高价值菌株的获得。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2220164

Zengcai Liu, Ruipeng Liu, Li Zou

{"title":"Development of a Transformation System for the Medicinal Fungus Sanghuangporus baumii and Acquisition of High-Value Strain.","authors":"Zengcai Liu, Ruipeng Liu, Li Zou","doi":"10.1080/12298093.2023.2220164","DOIUrl":"https://doi.org/10.1080/12298093.2023.2220164","url":null,"abstract":"To further explore the molecular mechanism of triterpenoid biosynthesis and acquire high-value strain of Sanghuangporus baumii, the Agrobacterium tumefaciens-mediated transformation (ATMT) system was studied. The key triterpenoid biosynthesis-associated gene isopentenyl diphosphate isomerase (IDI) was transformed into S. baumii by ATMT system. Then, the qRT-PCR technique was used to analyze gene transcript level, and the widely targeted metabolomics was used to investigate individual triterpenoid content. Total triterpenoid content and anti-oxidant activity were determined by spectrophotometer. In this study, we for the first time established an efficient ATMT system and transferred the IDI gene into S. baumii. Relative to the wild-type (WT) strain, the IDI-transformant (IT) strain showed significantly higher transcript levels of IDI and total triterpenoid content. We then investigated individual triterpenoids in S. baumii, which led to the identification of 10 distinct triterpenoids. The contents of individual triterpenoids produced by the IT2 strain were 1.76-10.03 times higher than those produced by the WT strain. The triterpenoid production showed a significant positive correlation with the IDI gene expression. Besides, IT2 strain showed better anti-oxidant activity. The findings provide valuable information about the biosynthetic pathway of triterpenoids and provide a strategy for cultivating high-value S. baumii strains.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/95/d1/TMYB_51_2220164.PMC10288903.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9714403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Characterization of Three Fusarium spp. Causing Wilt Disease of Cannabis sativa L. in Korea. 韩国三种引起大麻枯萎病的镰刀菌的鉴定。

IF 1.9 4区生物学

Mycobiology Pub Date : 2023-01-01 DOI: 10.1080/12298093.2023.2213911

Young Mo Koo, S M Ahsan, Hyong Woo Choi

{"title":"Characterization of Three Fusarium spp. Causing Wilt Disease of Cannabis sativa L. in Korea.","authors":"Young Mo Koo, S M Ahsan, Hyong Woo Choi","doi":"10.1080/12298093.2023.2213911","DOIUrl":"https://doi.org/10.1080/12298093.2023.2213911","url":null,"abstract":"In July 2021, wilting symptoms were observed in adult and seedling hemp (Cannabis sativa L. cv. Cherry Blossom) plants grown in a greenhouse. As the disease progressed, yellowing and wilting symptoms on the leaves developed, resulting in whole plant death. In seedling plants, typical damping-off symptoms were observed. To identify the pathogen, the roots of diseased plants were sampled, surface sterilized, and cultured on potato dextrose agar (PDA) media. From the culture, 4 different fungal isolates were recovered and purely cultured. Each fungal isolate showed distinct growth shapes and color development on malt extract agar, oatmeal agar, sabouraud dextrose agar, and PDA media. Microscopic observation and molecular identification using ribosomal DNA internal transcribed spacer sequencing identified them as 3 Fusarium spp. and 1 Thielaviopsis paradoxa. Additional sequencing of elongation factor 1-alpha and β-tubulin regions of 3 Fusarium spp. revealed that 2 of them are Fusarium solani, and the other one is Fusarium proliferatum. To examine which isolate can act as a causal agent of wilt disease of hemp, each isolate was tested for their pathogenicity. In the pathogenicity test, F. solani AMCF1 and AMCF2, and F. proliferatum AMCF3, but not T. paradoxa AMCF4, were able to cause wilting disease in hemp seedlings. Therefore, we report that F. solani AMCF1 and AMCF2, and F. proliferatum AMCF3 as causal agents of Fusarium wilt of hemp plants. To our knowledge, this is the first report of the wilt disease of C. sativa L. caused by Fusarium spp. in Korea.","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/03/ae/TMYB_51_2213911.PMC10288908.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9714405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0