Molecular Breeding最新文献

{"title":"Four QTLs control stigma exsertion rate by changing stigma size in rice","authors":"Quanya Tan, Zhenpeng Gan, Liang Xiong, Lin Shao, Weifeng Yang, Xin Luan, Guodong Chen, Fangping Li, Yuerong Ni, Haitao Zhu, Guifu Liu, Suhong Bu, Shaokui Wang, Guiquan Zhang","doi":"10.1007/s11032-024-01499-0","DOIUrl":"https://doi.org/10.1007/s11032-024-01499-0","url":null,"abstract":"<p>The stigma exsertion rate (SER) is a key trait for the outcrossing ability of hybrid rice, which directly affects the yield of hybrid seeds in hybrid seed production. In previous studies, we have located 18 QTLs for SER using single-segment substitution lines in rice. In this study, we found that 4 of 18 QTLs for SER controlled stigma size (SS). On chromosome 1, a QTL <i>qSL-1</i> controlling stigma length (SL) was located at the same interval of <i>qSER-1b</i>. On chromosome 2, two QTLs for SS, <i>qSS-2a</i> and <i>qSS-2b</i>, linked closely within a 1288.0 kb region, were at the same positions of <i>qSER-2a</i> and <i>qSER-2b</i>, respectively. A QTL <i>qSL-12</i> controlling SL on chromosome 12 was at the same location of <i>qSER-12</i>. Additive effects of four QTLs for SS ranged from 0.12 mm to 0.38 mm, showing significant effects on SS. In pyramiding lines of QTLs for SS, SS enlarged with the increase of QTLs. The effect of QTLs on SER was consistent with their effect on SS, and SL had a greater positive effect on SER than the stigma width. Our findings demonstrate that SS is one of the important factors affecting SER in rice.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142226123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid breeding of an early maturing, high-quality, and high-y.ielding rice cultivar using marker‑assisted selection coupled with optimized anther culture.","authors":"Junxiao Chen, Sanhe Li, Lei Zhou, Wenjun Zha, Huashan Xu, Kai Liu","doi":"10.1007/s11032-024-01495-4","DOIUrl":"10.1007/s11032-024-01495-4","url":null,"abstract":"<p><p>With the global shift towards healthier eating habits, the focus of the rice industry has evolved from quantity to quality. In China, the Yangtze River Basin is the main area consuming long-grain and high-quality indica rice. Hubei Province, a significant rice-producing area, currently cultivates a limited range of rice varieties, risking degradation and diminishing economic returns. Therefore, it is imperative to cultivate elite rice varieties tailored to the local production conditions and can significantly enhance the added value. This study bred the novel rice cultivar \"Runxiangyu\", characterized by early maturity, high quality, and high yield. It is a hybrid of Ezhong 5, known for its moderate height and excellent quality, albeit with a long growth period and lack of fragrance, and Yuzhenxiang, renowned for its high quality, short growth period, and fragrance but limited by its tall stature and poor tillering ability. The breeding process utilized optimized anther culture coupled with molecular marker-assisted selection (MAS) and phenotype analysis. In the field, the developed cultivar was 120.9 cm tall and had an entire growth period of 117.5 days, demonstrating moderate disease resistance and excellent heat tolerance. Its grains are fragrant, meeting the national standard of grade two high-quality rice set by the Food Quality Supervision and Inspection Center of the Ministry of Agriculture and Rural Areas). Exhibiting superior agronomic traits, such as plant type, height, growth period, and stress resistance, along with and quality attributes, including grain shape, chalkiness, fragrance, and taste, \"Runxiangyu\" was certified by the Agricultural Crop Variety Certification Commission of Hubei in 2022. These findings suggested that molecular MAS coupled with optimized anther culture and multi-site phenotype analysis is an efficient and rapid method for crop breeding.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01495-4.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 9","pages":"58"},"PeriodicalIF":2.6,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11377382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142154598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of quantitative trait loci from DJ123 (<i>aus</i>) independently affecting panicle structure traits in <i>indica</i> rice cultivar IR64.","authors":"Yoshiaki Ueda, Katsuhiko Kondo, Hiroki Saito, Juan Pariasca-Tanaka, Hideki Takanashi, Harisoa Nicole Ranaivo, Mbolatantely Rakotondramanana, Matthias Wissuwa","doi":"10.1007/s11032-024-01494-5","DOIUrl":"10.1007/s11032-024-01494-5","url":null,"abstract":"<p><p>The rice panicle is the principal organ to influence productivity and traits affecting panicle architecture determine sink size and yield potential. Improving panicle architecture may be effective in increasing yield under low-input conditions, but which traits are of importance under such conditions and how they are genetically controlled is not well understood. Using recombinant inbred lines (RILs) derived from a cross between a modern variety IR64 and a low fertility tolerant accession DJ123, quantitative trait locus (QTL) mapping was conducted under high soil fertility in Japan and low fertility in Madagascar. Among QTL for panicle length (PL) detected, the DJ123 allele increased rachis length at <i>qCL1</i> and <i>qPL9</i>, while the IR64 allele increased primary branch length at <i>qPL7</i>. DJ123 further contributed two QTL for grain width whereas IR64 contributed two grain length QTL. Analysis of lines carrying different combinations of detected QTL indicates that rachis and primary branch lengths are independently regulated, explaining strong transgressive segregation for PL. The positive effects of PL-related QTL were further confirmed by a genome-wide analysis of allelic states in two breeding lines that had been selected repeatedly for total panicle weight per plant under low input conditions. This study provides the genetic basis for complex panicle architecture in rice and will aid in designing an ideal panicle architecture that leads to increased yield under low fertility conditions.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01494-5.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 9","pages":"57"},"PeriodicalIF":2.6,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11366739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142126160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotype selection identified elite lines through quantitative trait loci mapping of agronomically important traits in wheat.","authors":"Yunlong Pang, Liming Wang, Linzhi Li, Xiaoqian Wang, Danfeng Wang, Meng Zhao, Chenhao Ma, Huirui Zhang, Qiang Yan, Yue Lu, Yunlong Liang, Xiangsheng Kong, Huaqiang Zhu, Xuecheng Sun, Yujie Zhao, Shubing Liu","doi":"10.1007/s11032-024-01496-3","DOIUrl":"10.1007/s11032-024-01496-3","url":null,"abstract":"<p><p>Wheat is one of the most important staple foods in the world. Genetic characterization of wheat agronomically important traits is crucial for yield improvement through molecular breeding. In this study, a recombinant inbred line (RIL) population was developed by crossing a local adapted high yield variety Jimai 22 (JM22) with an external variety Cunmai no.1 (CM1). A high-density genetic map containing 7,359 single nucleotide polymorphism (SNP) markers was constructed. Quantitative trait loci (QTL) mapping identified 61 QTL for eight yield-related traits under six environments (years). Among them, 17 QTL affecting spike number per plant, grain number per spike and thousand grain weight showed high predictability for theoretical yield per plant (TYP), of which, 12 QTL alleles positively contributed to TYP. Nine promising candidate genes for seven of the 12 QTL were identified including three known wheat genes and six rice orthologs. Four elite lines with TYP increased by 5.6%-15.2% were identified through genotype selection which carried 7-9 favorable alleles from JM22 and 2-3 favorable alleles from CM1 of the 12 QTL. Moreover, the linked SNPs of the 12 QTL were converted to high-throughput kompetitive allele-specific PCR (KASP) markers and validated in the population. The mapped QTL, identified promising candidate genes, developed elite lines and KASP markers are highly valuable in future genotype selection to improve wheat yield.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01496-3.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 9","pages":"56"},"PeriodicalIF":2.6,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142109597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Introgression of tetraploid <i>Thinopyrum elongatum</i> 6EL segments enhances the stripe rust resistance of adult wheat plants.","authors":"Chunyan Zeng, Liangxi Li, Zaimei He, Wei Zhu, Lili Xu, Yiran Cheng, Yi Wang, Jian Zeng, Xing Fan, Lina Sha, Haiqin Zhang, Guoyue Chen, Yonghong Zhou, Dandan Wu, Houyang Kang","doi":"10.1007/s11032-024-01493-6","DOIUrl":"10.1007/s11032-024-01493-6","url":null,"abstract":"<p><p>Preventing the widespread occurrence of stripe rust in wheat largely depends on the identification of new stripe rust resistance genes and the breeding of cultivars with durable resistance. In previous study, we reported 6E of wheat-tetraploid <i>Thinopyrum elongatum</i> 6E (6D) substitution line contains adult-stage stripe rust resistance genes. In this study, three novel wheat-tetraploid <i>Th. elongatum</i> translocation lines were generated from the offspring of a cross between common wheat and the 6E (6D) substitution line. Genomic in situ hybridization (GISH), fluorescence in situ hybridization chromosome painting (FISH painting), repetitive sequential FISH, and 55 K SNP analyses indicated that K227-48, K242-82, and K246-6 contained 42 chromosomes and were 6DL·6ES, 2DL·6EL, and 6DS·6EL translocation lines, respectively. The assessment of stripe rust resistance revealed that K227-48 was susceptible to a mixture of <i>Pst</i> races, whereas the 6EL lines K242-82 and K246-6 were highly resistance to stripe rust at the adult stage. Thus, this resistance was due to the chromosome arm 6EL of tetraploid <i>Th. elongatum</i>. The improved agronomic performance of 6DS·6EL translocation line may be a useful novel germplasm resource for wheat breeding programs. For the application of marker-assisted selection (MAS), 47 simple sequence repeat (SSR) markers were developed, showing specific amplification on the chromosome 6E using the whole-genome sequence of diploid <i>Th. elongatum</i>. The 6DS·6EL translocation line and SSR markers have the potential to be deploy for future stripe rust resistance wheat breeding program.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01493-6.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 8","pages":"55"},"PeriodicalIF":2.6,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11327235/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breaking the association between gametocidal gene(s) and leaf rust resistance gene (<i>LrS2427</i>) in <i>Triticum aestivum</i>-<i>Aegilops speltoides</i> derivative by gamma irradiation.","authors":"R Ragini, Niranjana Murukan, Navpreet Kaur Sekhon, Chetna Chugh, Priyanka Agarwal, Prachi Yadav, Niharika Mallick, Shailendra Kumar Jha, Mir Asif Iquebal, Gitanjali Tandon, Aakriti Verma, Bhupinder Singh, Sherry Rachel Jacob, K Raghunandan, Kumble Vinod Prabhu, Shivmangal Singh Tomar, Vinod","doi":"10.1007/s11032-024-01491-8","DOIUrl":"10.1007/s11032-024-01491-8","url":null,"abstract":"<p><p>Utilization of crop wild relatives of wheat can be very effective in building the genetic diversity to cater to the evolving strains of disease pathogens. <i>Aegilops speltoides</i> is a rich source of rust resistance genes however transferring those to wheat genome can be tedious due to co-transfer and preferential transmission of undesirable genes causing gametocidal activity. Such an unholy association was observed in <i>Triticum aestivum</i>-<i>Ae. speltoides</i> derivative line Sel. 2427 which possess the broad-spectrum leaf rust seedling resistance gene (<i>LrS2427</i>). Molecular analysis based on 35 K wheat breeder's array revealed the maximum percentage of <i>Ae. speltoides</i> genome introgression on homoeologous group 2. In situ hybridization studies revealed the presence of S genome in Sel. 2427, showing six translocations on four chromosomes. Karyotyping using repetitive probe (AAG)₆ revealed that the two chromosomes involved are 2D and 2B. Genic regions causing gametocidal activity were identified by dissecting it into component traits and QTLs on 2D and 2B chromosomes were revealed in case of the trait seed shrivelling index. To break the inadvertent association of <i>LrS2427</i> with gametocidal genes, F₁(Agra Local X Sel. 2427) seeds were irradiated with gamma rays and stable leaf rust resistant mutants lacking gametocidal activity were developed. These mutants showed resistance to different races of leaf rust pathogen and showed superior agronomic performance as well. These mutants could be a great resource in wheat improvement for utilization of the leaf rust resistance gene <i>LrS2427</i> without any yield penalty.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01491-8.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 8","pages":"54"},"PeriodicalIF":2.6,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11322474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141988379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>WRKY75</i> regulates anthocyanin accumulation in juvenile citrus tissues.","authors":"Zhihao Lu, Jiaxian He, Jialing Fu, Yuping Huang, Xia Wang","doi":"10.1007/s11032-024-01490-9","DOIUrl":"10.1007/s11032-024-01490-9","url":null,"abstract":"<p><p>The anthocyanin accumulation in juvenile tissues can enhance the ornamental value, attract pollinators, and help improve abiotic stress. Although transcriptional regulation studies of anthocyanin have been relatively extensive, there are few reports on the mechanism of anthocyanin accumulation in young tissues. This study reveals that many juvenile citrus tissues (flowers, leaves, and pericarp) undergo transient accumulation of anthocyanins, exhibiting a red coloration. Using weighted gene co-expression network analysis (WGCNA) identified <i>CitWRKY75</i> as a candidate gene. After detecting the expression levels of <i>CitWRKY75</i> in various citrus juvenile tissues, the expression trend of <i>CitWRKY75</i> was highly consistent with the red exhibiting and fading. Overexpression of <i>CitWRKY75</i> in tobacco significantly increased the anthocyanin content. LUC and yeast one-hybrid assay demonstrated that <i>CitWRKY75</i> could bind to the promoter of <i>CitRuby1</i>(encoding the key transcription factor promoting anthocyanin accumulation) and promote its expression. Finally, comparing the expression levels of <i>CitWRKY75</i> and <i>CitRuby1</i> in the late development stage of blood orange found that <i>CitWRKY75</i> was not the main regulatory factor for anthocyanin accumulation in the later stage. This study used reverse genetics to identify a transcription factor, CitWRKY75, upstream of <i>CitRuby1</i>, which promotes anthocyanin accumulation in citrus juvenile tissues.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01490-9.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 8","pages":"52"},"PeriodicalIF":2.6,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11315850/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141917129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Map-based cloning reveals <i>Cpgp</i> gene encoding an APRR2 protein to regulate the green fruit peel formation in <i>Cucurbita pepo</i>.","authors":"Kiros Gebretsadik, Wanlu Chen, Ying Duan, Yapei Sun, Yadi He, Qinggui Liao, Changlin Wang, Kailiang Bo","doi":"10.1007/s11032-024-01492-7","DOIUrl":"10.1007/s11032-024-01492-7","url":null,"abstract":"<p><p>Fruit peel color is a major factor that influences fruit quality and customers' demand. However, the molecular mechanisms underlying the green fruit peel color trait of <i>Cucurbita pepo</i> L. remain unknown. Two parental lines, RP16 and RP38, were used to study the fruit peel color trait in <i>C. pepo</i>. The parental line RP16 shows white peel color, whereas RP38 exhibits green peel color. 384 F₂ populations were used to identify the inheritance pattern associated with green fruit and white fruit peel in <i>Cucurbita pepo</i> L. 293 F₂ individuals were white, and 91 F₂ individuals were green, resulting in a ratio of 3:1. Hence, white peel is dominant over the green fruit peel trait, and a single recessive green peel gene (<i>Cpgp</i>) controls the green fruit peel. The fruit chlorophyll (Chll) content decreases as fruit matures in the RP16 line. In contrast, Chll increases during the fruit growing periods on fruit peels of the RP38 line. The BSA-sequence analysis revealed the <i>Cpgp</i> locus on Chr5, within a 2.3 Mb region. Subsequent fine-mapping analysis, using 699 F₂ plants, narrowed down this region to 23.90 kb on the same chromosome. Within this region, two annotated genes, namely <i>Cp4.1LG05g02070</i> and <i>Cp4.1LG05g02060</i>, are present. These genes are predicted to encode a two-component Arabidopsis Pseudo-Response Regulator 2-like protein (APRR2), which may be involved in green pigmentation processes in plants. Consequently, sequence alignment and gene expression analyses at various fruit development stages supported that <i>Cp4.1LG05g02070</i> may be the primary candidate gene responsible for regulating the green fruit peel color trait in <i>Cucurbita pepo</i> L. This study may provide a basis for further study on the basic mechanisms that control the fruit peel colors in <i>Cucurbita spp</i>.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01492-7.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 8","pages":"53"},"PeriodicalIF":2.6,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11315825/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141917158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single- and multiple-trait quantitative trait locus analyses for seed oil and protein contents of soybean populations with advanced breeding line background.","authors":"Tu Huynh, Kyujung Van, M A Rouf Mian, Leah K McHale","doi":"10.1007/s11032-024-01489-2","DOIUrl":"10.1007/s11032-024-01489-2","url":null,"abstract":"<p><p>Soybean seed oil and protein contents are negatively correlated, posing challenges to enhance both traits simultaneously. Previous studies have identified numerous oil and protein QTLs via single-trait QTL analysis. Multiple-trait QTL methods were shown to be superior but have not been applied to seed oil and protein contents. Our study aimed to evaluate the effectiveness of single- and multiple-trait multiple interval mapping (ST-MIM and MT-MIM, respectively) for these traits using three recombinant inbred line populations from advanced breeding line crosses tested in four environments. Using original and simulated data, we found that MT-MIM did not outperform ST-MIM for our traits with high heritability (H² > 0.84). Empirically, MT-MIM confirmed only five out of the seven QTLs detected by ST-MIM, indicating single-trait analysis was sufficient for these traits. All QTLs exerted opposite effects on oil and protein contents with varying protein-to-oil additive effect ratios (-0.4 to -4.8). We calculated the economic impact of the allelic variations via estimated processed values (EPV) using the National Oilseed Processors Association (NOPA) and High Yield + Quality (HY + Q) methods. Oil-increasing alleles had positive effects on both EPV_NOPA and EPV_HY+Q when the protein-to-oil ratio was low (-0.4 to -0.7). However, when the ratio was high (-4.1 to -4.8), oil-increasing alleles increased EPV_NOPA and decreased EPV_HY+Q, which penalizes low protein meal. In conclusion, single-trait QTL analysis is adequately effective for high heritability traits like seed oil and protein contents. Additionally, the populations' elite pedigrees and varying protein-to-oil ratios provide potential lines for further yield assessment and direct integration into breeding programs.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01489-2.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 8","pages":"51"},"PeriodicalIF":2.6,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11306453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"QTL detection and candidate gene identification of qCTB1 for cold tolerance in the Yunnan plateau landrace rice","authors":"Haifeng Guo, Yongmei Guo, Yawen Zeng, Andong Zou, Najeeb Ullah Khan, Yunsong Gu, Jin Li, Xingming Sun, Zhanying Zhang, Hongliang Zhang, Youliang Peng, Huahui Li, Zhigang Wu, Pingrong Yuan, Jinjie Li, Zichao Li","doi":"10.1007/s11032-024-01488-3","DOIUrl":"https://doi.org/10.1007/s11032-024-01488-3","url":null,"abstract":"<p>Cold stress is one of the main abiotic stresses that affects rice growth and production worldwide. Dissection of the genetic basis is important for genetic improvement of cold tolerance in rice. In this study, a new source of cold-tolerant accession from the Yunnan plateau, Lijiangxiaoheigu, was used as the donor parent and crossed with a cold-sensitive cultivar, Deyou17, to develop recombinant inbred lines (RILs) for quantitative trait locus (QTL) analysis for cold tolerance at the early seedling and booting stages in rice. In total, three QTLs for cold tolerance at the early seedling stage on chromosomes 2 and 7, and four QTLs at the booting stage on chromosomes 1, 3, 5, and 7, were identified. Haplotype and linear regression analyses showed that QTL pyramiding based on the additive effect of these favorable loci has good potential for cold tolerance breeding. Effect assessment in the RIL and BC₃F₃ populations demonstrated that <i>qCTB1</i> had a stable effect on cold tolerance at the booting stage in the genetic segregation populations. Under different cold stress conditions, <i>qCTB1</i> was fine-mapped to a 341-kb interval between markers M3 and M4. Through the combination of parental sequence comparison, candidate gene-based association analysis, and tissue and cold-induced expression analyses, eight important candidate genes for <i>qCTB1</i> were identified. This study will provide genetic resources for molecular breeding and gene cloning to improve cold tolerance in rice.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"71 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141782644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}