Molecular Breeding最新文献

{"title":"Kemai 1609, a molecular designed wheat variety with stripe rust and leaf rust resistance.","authors":"Yongxing Chen, Xiuhua Zhu, Qiuhong Wu, Lingli Dong, Huaizhi Zhang, Hongkui Fu, Ping Lu, Miaomiao Li, Guanghao Guo, Keyu Zhu, Gaojie Wang, Chengguo Yuan, Hongjie Li, Xicheng Wang, Tingjie Cao, Zhiyong Liu","doi":"10.1007/s11032-025-01593-x","DOIUrl":"10.1007/s11032-025-01593-x","url":null,"abstract":"","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 9","pages":"69"},"PeriodicalIF":3.0,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12373560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial candidate gene, <i>orf206</i>, for cytoplasmic male sterility in Pear (<i>Pyrus pyrifolia</i>).","authors":"Hyeondae Han, Sewon Oh, Daeil Kim","doi":"10.1007/s11032-025-01591-z","DOIUrl":"10.1007/s11032-025-01591-z","url":null,"abstract":"<p><p>Cytoplasmic male sterility (CMS) caused by mitochondrial genome alterations in flowering plants plays a crucial role in hybrid breeding systems. In pear (<i>Pyrus</i> spp.), pollenless phenotypes have been consistently observed in progeny. However, the genetic basis and inheritance mechanisms of male sterility in pears remain poorly understood. To investigate the inheritance mode, we performed segregation analysis in four F₁ populations derived from crosses using a cultivar carrying CMS-type cytoplasm as the maternal parent. The observed male sterility segregation ratios confirmed a maternal inheritance pattern consistent with the CMS model and suggested differential effects of nuclear fertility restorer genes from various pollen parents. We analyzed whole-genome sequencing data from four pear accessions, identifying an 860 bp mitochondrial DNA sequence associated with male-sterile individuals. This sequence was located near <i>cox3</i> and <i>apt8</i>, commonly co-located with CMS loci in other plant species. Within this region, we identified <i>orf206</i>, a chimeric open reading frame composed of 113 bp from <i>nad3</i> in <i>Pyrus betulifolia</i> and 403 bp from <i>atp9-1</i> of <i>Malus × domestica</i>. The predicted protein encoded by <i>orf206</i> contained three transmembrane domains, which are typical features of CMS-associated proteins. Our results demonstrate that male sterility in pears is maternally inherited and support <i>orf206</i> as a strong candidate gene for CMS induction. Furthermore, we developed an InDel marker (CBpMtid03 and CBpMtid07) targeting the CMS-specific mitochondrial sequence enabling the efficient identification of CMS individuals in breeding programs. These findings provide insights into the molecular mechanisms underlying pollen sterility in pears and facilitate marker-assisted selection in pear breeding.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01591-z.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 8","pages":"68"},"PeriodicalIF":3.0,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144874182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Image-based GWAS identifies the genetic architecture of seed-related traits in a soybean mutant population.","authors":"Jung Min Kim, Jeong Woo Lee, Dae June Kim, Jae Il Lyu, JeongHo Baek, Bo-Keun Ha, Soon-Jae Kwon","doi":"10.1007/s11032-025-01584-y","DOIUrl":"https://doi.org/10.1007/s11032-025-01584-y","url":null,"abstract":"<p><p>Soybean [<i>Glycine max</i> (L.) Merr.] seed morphology markedly influences yield, productivity, and nutritional value. However, assessing quantitative traits remains challenging due to their complexity and strong genotype-by-environment interactions. In this study, a high-throughput phenotyping (HTP) system was used to evaluate 13 image-based traits and a hundred-seed weight in a soybean mutant diversity pool (MDP) comprising 192 genotypes. All traits exhibited significant variations within the mutant diversity pool across multiple environments. Correlation analysis revealed strong positive and negative correlations among the traits regarding seed size, shape, color, and weight. Genome-wide association studies (GWAS) were conducted using 37,249 single nucleotide polymorphisms (SNPs) generated through genotype-by-sequencing (GBS) to uncover the genetic architecture of seed-related traits. The image-based GWAS identified 79 significant quantitative trait nucleotides (QTNs) that were simultaneously detected under all environments. Notably, five novel pleiotropic QTNs were consistently mapped to chromosomes 7, 10, 15, 18, and 20, each associated with a specific candidate gene. These genes exhibited marked expression differences during the seed developmental stages between the wild-type cultivar and its mutant. The HTP-integrated GBS demonstrates a powerful approach for precise trait dissection and genomic selection. These findings provide critical insights into the genetic architecture underlying desirable seed morphology and offer valuable tools for advancing precision soybean breeding.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01584-y.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 8","pages":"67"},"PeriodicalIF":3.0,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12339847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144847648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Navigating the genetic basis of cadmium accumulation: a comprehensive analysis of low-cadmium accumulation rice.","authors":"Deyi Shao, Lixin Yin, Jian Zhao, Zhengliang Luo, Weiguo Li, Yongka Wang, Xiangjie Liu, Bingchuan Tian, Xiaobo Long, Hexing Yin, Kun Zhou","doi":"10.1007/s11032-025-01575-z","DOIUrl":"10.1007/s11032-025-01575-z","url":null,"abstract":"<p><p>The presence of cadmium (Cd) in rice poses a significant health risk to consumers, highlighting the urgency of breeding rice varieties with low Cd accumulation. To identify genetic resources and potential genes for developing such rice varieties, a comprehensive genome-wide association study (GWAS) was conducted on 158 rice varieties, which tested between 2021 and 2023 in low cadmium accumulation testing framework, to identify candidate genes associated with cadmium content in brown rice. Based on their parental origin and genetic population structure analysis, we categorized these 158 varieties into four subgroups: Luohong, <i>lcd1</i>, intermediate and early indica series. Specifically, the four subgroups of low cadmium varieties were breeded based on <i>OsNramp5</i> mutants Luohong 3A/4A, <i>lcd1</i>, Lian 1S and Shaoxiang 100, respectively. GWAS analysis identified sixteen loci significantly associated with cadmium content, twelve of which showed consistent associations across multiple environments, these loci were mapped to chromosomes 1, 2, 5, 7, 11, and 12, suggesting their potential for further fine mapping and functional validation. Through gene function annotation analysis, candidate genes related to cadmium content in these loci were identified, including <i>Os05 g0382200</i>, <i>Os07 g0232800</i> (<i>OsZIP8</i>), <i>Os07 g0232900</i> (<i>OsHMA3</i>), <i>Os07 g0257200</i> (<i>OsNramp5</i>), <i>Os07 g0258400</i> (<i>OsNramp1</i>), <i>Os12 g0512100</i>, <i>Os12 g0512700</i>, and <i>Os12 g0514000</i>. These genes are implicated in the absorption, transport, and accumulation of heavy metals, particularly cadmium. Haplotype analysis of key genes <i>OsZIP8</i>, <i>OsHMA3</i>, <i>OsNramp5</i>, and <i>OsNramp1</i> identified specific low-cadmium dominant haplotypes. Notably, <i>OsHMA3</i>-Hap2 (GC), <i>OsNramp5</i>-Hap1 (DEL), and <i>OsNramp1</i>-Hap1 (DEL) were associated with Luohong-origin varieties, while <i>OsHMA3</i>-Hap1 (AC), <i>OsNramp5</i>-Hap2 (AA), and <i>OsNramp1</i>-Hap2 (GGG) were linked to <i>lcd1</i>-origin varieties. Overall, this study illustrated the genetic basis for breeding low-cadmium rice varieties and provided candidate loci to develop molecular markers to enhance food safety through reduced heavy metal content.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01575-z.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 8","pages":"66"},"PeriodicalIF":3.0,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12317945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144775828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of novel genomic regions associated with yield-related traits in cowpea (<i>Vigna unguiculata</i> [L.] Walp) landraces.","authors":"Lei Han, Baogen Wang, Xiaohua Wu, Xiaoyang Chen, Xiao Li, Ying Wang, Jian Wang, Qingya Tang, Zhongfu Lu, Guojing Li, Yong He, Xinyi Wu","doi":"10.1007/s11032-025-01585-x","DOIUrl":"https://doi.org/10.1007/s11032-025-01585-x","url":null,"abstract":"<p><p>Cowpea is an important multipurpose legume crop that used for food, feed and vegetable worldwide. Developing the high yield cultivars is the first target in cowpea breeding, however, the genetic basis of this complex trait is not yet well understood. To discover the genetic architecture of cowpea yield, a total of 215 cowpea landraces collected from Zhejiang Province were evaluated for four yield-related traits including branch number per plant (BNP), grain number per pod (GNP), pod length (PL), and pod number per plant (PNP). By resequencing this diversity panel, total of 3,880,169 high-confidence single nucleotide polymorphisms (SNPs) were identified, population structure analysis showed that these cowpea landraces were classified into four subpopulations and the subpopulation division was highly related to the pod length and pod-type. Through conducting a GWAS on the four traits, 24 genomic regions significantly associated with cowpea yield were detected and haplotype analysis showed the favorable genotypes of each locus has stronger genetic effect on the yield-related traits. Based on the cowpea G98 reference genome, six predicated genes (<i>VuG9806G022730</i>, <i>VuG9809G015960</i>, <i>VuG9801G022820</i>, <i>VuG9801G008990</i>, <i>VuG9801G016500</i>, <i>VuG9807G013020</i>) were identified as the likely candidate genes for BNP_6.2, BNP_9.1, GNP_1.1, PL_1.1, PNP_1.2 and PNP_7.1, respectively, which involving in multiple pathways such as auxin response and regulation, cell expansion and ovary development. These results will facilitate the molecular breeding of high yield cultivars in cowpea and benefit for improving the global food security and the nutritional structure of human diets.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01585-x.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 8","pages":"65"},"PeriodicalIF":3.0,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12307267/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144760472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of SNP panel for genetic diversity assessment, fingerprinting identification and backcross breeding in <i>Brassica oleracea</i>.","authors":"Xueqin Yao, Feng Hong, Guangqing Li, Lei Huang, Chunqing Liu, Jing Gong, Bo Wang, Juanjuan Li, Kede Liu, Zhujie Xie","doi":"10.1007/s11032-025-01586-w","DOIUrl":"10.1007/s11032-025-01586-w","url":null,"abstract":"<p><p>Broccoli (<i>Brassica oleracea</i> L. var. <i>italica</i>) is a globally important vegetable due to its rich nutrients as well as its anti-cancer effect. China is the world's largest producer and exporter of broccoli. However, since the research on commercial breeding of broccoli in China started relatively late, the level of genetic breeding in our country lags behind with more than 80% seeds imported. To assist broccoli breeding with molecular markers, we re-sequenced 41 representative broccoli inbred lines at high coverage depth and identified a total of 1,348,968 SNPs. From these SNPs, a genotyping-in-thousand by sequencing (GT-seq) SNP panel composed of 700 evenly distributed high-quality SNPs was developed. We assessed the genetic diversity, population structure, and kinship of 114 <i>B. oleracea</i> varieties bred in different institutions including broccolis, cabbages, cauliflowers and kales with this SNP panel, and found that the genetic diversity of these varieties was somewhat limited, with an average heterozygosity of 18.35% and an average Polymorphic Information Content (PIC) of 0.26. Population structure analysis divided the varieties into two main groups, consistent with the origin from two independent domestication events. The SNP panel was also employed to screen individuals with high background recovery rates in backcross breeding. Furthermore, the SNP panel was used to test seed purity of parental inbred lines and F1 hybrids, which could expedite the entry of hybrid seeds into the market. Overall, the developed GT-seq SNP panel is a valuable tool for various aspects of <i>B. oleracea</i> breeding and genetics studies.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01586-w.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 8","pages":"64"},"PeriodicalIF":3.0,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12297122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of QTLs for adult-plant stripe rust resistance in Chinese wheat landrace Yizhanghongkemai and assessment of their utility for decreasing yield loss.","authors":"Yumei Li, Jiaru Yang, Jing Zhang, Shuanglin Du, Hongli Ji, Zehou Liu, Hao Tang, Peixun Liu, Qin Wang, Haiqin Zhang, Wuyun Yang, Jun Li, Hongshen Wan","doi":"10.1007/s11032-025-01583-z","DOIUrl":"https://doi.org/10.1007/s11032-025-01583-z","url":null,"abstract":"<p><p>Stripe rust is prevalent in the wheat-growing region of southwestern China. Frequent changes in stripe rust pathogen virulence in this region lead to a rapid loss of disease resistance among wheat varieties. However, Chinese wheat landrace Yizhanghongkemai (YZHK) has exhibited adult-plant stripe rust resistance for more than one decade in a disease nursery in southwestern China. To elucidate the underlying genetic basis, quantitative trait loci (QTLs) for adult-plant stripe rust resistance in YZHK were analyzed using an inclusive composite interval mapping method. Six QTLs for adult-plant stripe rust resistance were detected on chromosomes 1BL, 2BL, 3DS, 5BL, 5DL, and 7DS in multiple environments. Notably, <i>QYrYZHK.saas-1B</i>, <i>QYrYZHK.saas-2B</i> and <i>QYrCY.saas-5D</i> were likely new disease resistance loci. By comparing the effects of QTL alleles on yield and its related components in field trials in which stripe rust was severe and effectively controlled, we determined that three QTLs significantly decreased yield losses due to stripe rust, among which the QTLs on chromosomes 1BL and 7DS were from YZHK, whereas the QTL on chromosome 5DL was from the other parent Chuanyu 12. These QTLs represent elite genetic resources for developing wheat varieties with adult-plant stripe rust resistance in the wheat-growing region of southwestern China.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01583-z.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 7","pages":"61"},"PeriodicalIF":2.6,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12260146/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic analysis of trade-offs among disease resistance, yield, and quality traits employing genome-wide association mapping in indica rice (<i>Oryza sativa</i> L.).","authors":"Vinodkumar Naik Moode, Madhusudhan Puchakayala, Srividya K Gannavarapu, Madhavilatha Kommana, Lalam Krishna, Sivarama Lekkala, Navajeet Chakravartty, VBReddy Lachagari, Shaik Nafeez Umar, Srividhya Akkareddy, Issa Keerthi, Sreelakshmi Chintala, Nirmalkumar R Amjikarai, Lakshminarayana R Vemireddy","doi":"10.1007/s11032-025-01578-w","DOIUrl":"https://doi.org/10.1007/s11032-025-01578-w","url":null,"abstract":"<p><p>The genetic trade-offs among complex traits are often witnessed in rice, however, very little is known about the contributing genes and mechanisms to exploit in breeding programmes. Here, we aimed to understand the genetic trade-offs among disease resistance, quality, and yield traits employing genome-wide association mapping. In all, 78 common marker-trait associations (MTAs) were identified for the targeted traits. In addition, five pleiotropic MTAs, 17 tightly linked MTAs, and two pleiotropic and tightly linked MTAs were detected for various trait combinations. The majority of MTA clusters were observed for quality traits (15 clusters) followed by the combined yield and quality traits (5 clusters) while only one cluster was found for combined yield and disease resistance traits. Further, the prediction of candidate genes controlling MTA clusters by exploiting the publicly available rice genome databases, revealed D-type cyclin 3;1 and Xyloglucan endotransglucosylase were found to be responsible for controlling grain size traits. We found no significant large linkage drag blocks with major MTAs for the targeted traits indicating that the indica rice genotypes have fewer trade-offs compared to japonica. The current study provides deeper insights into the genetic trade-offs among complex traits in rice, aiding in the meticulous planning of future breeding strategies.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01578-w.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 7","pages":"60"},"PeriodicalIF":2.6,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12246346/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and fine-mapping of <i>qNCLB3.04</i> resistant to Northern Corn Leaf Blight.","authors":"Junhua Wu, Wencai Yang, Xiangdong Shi, Bao Zhang, Min Jiang, Xin Qi, Jun Ma, Jennifer S Jaqueth, Bailin Li, Mingqiu Dai, Yunling Peng, Zhibing Lai","doi":"10.1007/s11032-025-01581-1","DOIUrl":"https://doi.org/10.1007/s11032-025-01581-1","url":null,"abstract":"<p><p>Northern Corn Leaf Blight (NCLB), caused by the fungal pathogen <i>Setosphaeria turcica</i>, is a destructive disease on maize. Identification of resistance quantitative trait loci (QTLs) or genes is crucial for breeding maize varieties with durable resistance to NCLB. Although a lot of resistance QTLs against NCLB have been isolated, only a few have been fine-mapped to date. Here, a BC₁F₁ population was developed from a cross between the resistance line CIMBL75 and the susceptible line Liao3162. This population was inoculated with mixed conidia of six <i>S. turcica</i> races. Through five field trials, five resistance QTLs against NCLB were identified in this BC₁F₁ population. One of them, <i>qNCLB3.04</i> on bin3.04, was repeatedly detected across all five trials. It explained 4.8-9.3% of phenotypic variation. Furthermore, the <i>qNCLB3.04</i> locus was narrowed down to a 5.053 Mb region by using a progeny-based sequential fine-mapping strategy. Hence, <i>qNCLB3.04</i> holds significant potential for improving maize broad-spectrum resistance against NCLB.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01581-1.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 7","pages":"59"},"PeriodicalIF":2.6,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12240901/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering saline-alkali-tolerant apple rootstocks by overexpressing <i>MdHYL1</i> in M9-T337.","authors":"Tianle Fan, Shufan Song, Ningning Bian, Fang Zhi, Fengwang Ma, Qingmei Guan, Xuewei Li","doi":"10.1007/s11032-025-01579-9","DOIUrl":"10.1007/s11032-025-01579-9","url":null,"abstract":"<p><p>Apple trees are frequently subjected to varying degrees of salt stress. HYL1, a key protein involved in miRNA biosynthesis, has been shown to play critical roles in plant responses to cold, drought, and pathogen infection. However, the specific function of MdHYL1 in mediating salt-alkali stress tolerance in apple remains unknown. In this study, we demonstrated that overexpression of <i>MdHYL1</i> in M9-T337 rootstocks significantly enhanced salt-alkali stress tolerance, including improved growth performance, reduced Na⁺/K⁺ ratio, decreased membrane damage, enhanced photosynthetic, and antioxidant capacity, which significantly impairs their growth, fruit quality, and yield. Moreover, scions grafted onto <i>MdHYL1</i> OE rootstocks displayed superior saline-alkali stress tolerance compared to those grafted onto M9-T337 rootstocks. Taken together, our findings highlight <i>MdHYL1</i> as a promising candidate gene for improving saline-alkali stress tolerance in fruit trees through biotechnological approaches.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01579-9.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 7","pages":"58"},"PeriodicalIF":2.6,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12198086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}