Molecular Breeding最新文献

{"title":"Genetic diversity and population structure of sweet corn in China as revealed by mSNP.","authors":"Quannv Yang, Zifeng Guo, Yunbi Xu, Yunbo Wang","doi":"10.1007/s11032-024-01533-1","DOIUrl":"10.1007/s11032-024-01533-1","url":null,"abstract":"<p><p>Corn is a widely grown cereal crop that serves as a model plant for genetic and evolutionary studies. However, the heterosis pattern of sweet corn remains unclear. Here, we analysed the genetic diversity and population structure of 514 sweet corn inbred lines and 181 field corn inbred lines. The population structure study enabled the classification of sweet corn into four groups: temperate sweet corns 1 and 2, tropical sweet corn, and subtropical sweet corn, in addition to the temperate and tropical field corn groups. Temperate sweet corn groups 1 and 2 were merged into the temperate sweet corn cluster in the phylogenetic trees. Principal component analysis divided sweet corn into four groups: temperate groups 1 and 2, tropical, and subtropical. Sweet corn exhibited lower levels of genetic diversity, polymorphism information content, and minor allele frequency than field corn. The average genetic distances and differentiation coefficients between inbreds within each sweet corn group were lower than those within field corn groups, indicating a relatively narrow genetic base in sweet corn. Taken together, the 514 sweet corn inbred lines can be divided into four groups: temperate 1, temperate 2, tropical, and subtropical. The classification of sweet corn groups in this study provides a reference for the breeding of sweet corn.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01533-1.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 1","pages":"6"},"PeriodicalIF":2.6,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671667/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic identification of the <i>NF-Y</i> gene family in apple and functional analysis of <i>MdNF-YB18</i> involved in flowering transition.","authors":"Cai Gao, Pengyan Wei, Zushu Xie, Pan Zhang, Muhammad Mobeen Tahir, Turgunbayev Kubanychbek Toktonazarovich, Yawen Shen, Xiya Zuo, Jiangping Mao, Dong Zhang, Yanrong Lv, Xiaoyun Zhang","doi":"10.1007/s11032-024-01524-2","DOIUrl":"10.1007/s11032-024-01524-2","url":null,"abstract":"<p><p>Apple is a crucial economic product extensively cultivated worldwide. Its production and quality are closely related to the floral transition, which is regulated by intricate molecular and environmental factors. <i>Nuclear factor Y</i> (<i>NF-Y</i>) is a transcription factor that is involved in regulating plant growth and development, with certain <i>NF-Ys</i> play significant roles in regulating flowering. However, there is little information available regarding <i>NF-Ys</i> and their role in apple flowering development. In the present study, 51 NF-Y proteins were identified and classified into three subfamilies, including 11 MdNF-YAs, 26 MdNF-YBs, and 14 MdNF-YCs, according to their structural and phylogenetic features. Further functional analysis focused on <i>MdNF-YB18.</i> Overexpression of <i>MdNF-YB18</i> in <i>Arabidopsis</i> resulted in earlier flowering compared to the wild-type plants. Subcellular localization confirmed <i>MdNF-YB18</i> was located in the nuclear. Interaction between MdNFY-B18 and MdNF-YC3/7 was demonstrated through yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays. Yeast one-hybrid (Y1H) and the dual-luciferase reporter assays showed MdNF-YB18 could bind the promoter of <i>MdFT1</i> and activate its expression. Moreover, this activation was enhanced with the addition of MdNF-YC3 and MdNF-YC7. Additionally, MdNF-YB18 also could interact with <i>MdCOLs</i> (<i>CONSTANS Like</i>). This study lays the foundation for exploring the functional traits of MdNF-Y proteins, highlighting the crucial role of <i>MdNF-YB18</i> in activating <i>MdFT1</i> in <i>Malus</i>.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01524-2.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 1","pages":"4"},"PeriodicalIF":2.6,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11668704/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of superior haplotypes and candidate gene for seed size-related traits in soybean (<i>Glycine max</i> L.).","authors":"Ye Zhang, Xinjing Yang, Javaid Akhter Bhat, Yaohua Zhang, Moran Bu, Beifang Zhao, Suxin Yang","doi":"10.1007/s11032-024-01525-1","DOIUrl":"10.1007/s11032-024-01525-1","url":null,"abstract":"<p><p>Seed size is an economically important trait that directly determines the seed yield in soybean. In the current investigation, we used an integrated strategy of linkage mapping, association mapping, haplotype analysis and candidate gene analysis to determine the genetic makeup of four seed size-related traits viz., 100-seed weight (HSW), seed area (SA), seed length (SL), and seed width (SW) in soybean. Linkage mapping identified a total of 23 quantitative trait loci (QTL) associated with four seed size-related traits in the F₂ population; among them, 17 were detected as novel QTLs, whereas the remaining six viz., <i>qHSW3-1</i>, <i>qHSW4-1</i>, <i>qHSW18-1</i>, <i>qHSW19-1</i>, <i>qSL4-1</i> and <i>qSW6-1</i> have been previously identified. Six out of 23 QTLs were major possessing phenotypic variation explained (PVE) ≥ 10%. Besides, the four QTL Clusters/QTL Hotspots harboring multiple QTLs for different seed size-related traits were identified on Chr.04, Chr.16, Chr.19 and Chr.20. Genome-wide association study (GWAS) identified a total of 62 SNPs significantly associated with the four seed size-related traits. Interestingly, the QTL viz., <i>qHSW18-1</i> was identified by both linkage mapping and GWAS, and was regarded as the most stable loci regulating HSW in soybean. <i>In-silico</i>, sequencing and qRT-PCR analysis identified the <i>Glyma.18G242400</i> as the most potential candidate gene underlying the <i>qHSW18-1</i> for regulating HSW. Moreover, three haplotype blocks viz., Hap2, Hap6A and Hap6B were identified for the SW trait, and one haplotype was identified within the <i>Glyma.18G242400</i> for the HSW. These four haplotypes harbor three to seven haplotype alleles across the association mapping panel of 350 soybean accessions, regulating the seed size from lowest to highest through intermediate phenotypes. Hence, the outcome of the current investigation can be utilized as a potential genetic and genomic resource for breeding the improved seed size in soybean.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01525-1.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 1","pages":"3"},"PeriodicalIF":2.6,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11663835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The pan-NLRome analysis based on 23 genomes reveals the diversity of NLRs in <i>Brassica napus</i>.","authors":"Weidong Ning, Wenzheng Wang, Zijian Liu, Weibo Xie, Hanchen Chen, Dengfeng Hong, Qing-Yong Yang, Shifeng Cheng, Liang Guo","doi":"10.1007/s11032-024-01522-4","DOIUrl":"10.1007/s11032-024-01522-4","url":null,"abstract":"<p><p><i>Brassica napus</i>, a globally significant oilseed crop, exhibits a wide distribution across diverse climatic zones. <i>B. napus</i> is being increasingly susceptible to distinct diseases, such as blackleg, clubroot and sclerotinia stem rot, leading to substantial reductions in yield. Nucleotide-binding site leucine-rich repeat genes (<i>NLRs</i>), the most pivotal family of resistance genes, can be effectively harnessed by identifying and uncovering their diversity to acquire premium disease-resistant gene resources. Here, we collected the genomes of 23 accessions and established the first comprehensive pan-NLRome in <i>B. napus</i> by leveraging multiple genomic resources. We observe significant variation in the number of <i>NLR</i> genes across different <i>B. napus</i> accessions, ranging from 189 to 474. Notably, <i>TNL</i> (TIR-NBS-LRR) genes constitute approximately half of the total count, indicating their predominant presence in <i>B. napus</i>. The number of <i>NLRs</i> in the C subgenome is significantly higher than that in the A subgenome, and chromosome C09 exhibits the highest density of <i>NLR</i> genes with featuring multiple <i>NLR</i> clusters. Domain analysis reveals that the integrated domains significantly enhance the diversity of NLRs, with B3 DNA binding, VQ, and zinc fingers being the most prevalent integrated domains. Pan-genomic analysis reveals that the core type of <i>NLR</i> genes, which is present in most accessions, constitutes approximately 58% of the total <i>NLRs</i>. Furthermore, we conduct a comparative analysis of the diversity of <i>NLR</i> genes across distinct ecotypes, leading to the identification of ecotype-specific NLRs and their integrated domains. In conclusion, our study effectively addresses the limitations of a single reference genome and provides valuable insights into the diversity of <i>NLR</i> genes in <i>B. napus</i>, thereby contributing to disease resistance breeding.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 12","pages":"2"},"PeriodicalIF":2.6,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11655762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abnormal transition from meiosis I to meiosis II induces male sterility in a seedless artificial hybrid of citrus.","authors":"Zhixiong Rao, Ruotian Sun, Shengjun Liu, Wanqi Ai, Lizhi Song, Xia Wang, Qiang Xu","doi":"10.1007/s11032-024-01521-5","DOIUrl":"10.1007/s11032-024-01521-5","url":null,"abstract":"<p><p>Male sterility is an important trait for breeding and for the seedless fruit production in citrus. We identified one seedling which exhibiting male sterility and seedlessness (named <i>ms1</i> hereafter), from a cross between two fertile parents, with sour orange (<i>Citrus aurantium</i>) as seed parent and Ponkan mandarin (<i>Citrus reticulata</i>) as pollen parent. Analysis using pollen viability staining, scanning electron microscopy (SEM), and transmission electron microscopy (TEM) revealed that the mature pollen of the <i>ms1</i> was aborted, displaying collapse and deformity. Further cytological analysis identified the abnormal formation of monad, dyad, and tetrad instead of the normal tetrad formation, leading to meiotic failure in the seedless hybrid. By comparative transcript profiling of meiotic anther of fertile and sterile hybrids, we observed significant downregulation of <i>CYCA1;2</i> (<i>TAM</i>) and <i>OSD1</i> genes in the hybrid, which known to control the transition from meiosis I to meiosis II in plants. These results indicated abnormal meiosis led to the male sterility of the seedless hybrid and that the decreased activities of kinases and cyclins may associated with the failure of the transition of meiosis I to meiosis II during anthers development.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01521-5.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 1","pages":"1"},"PeriodicalIF":2.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11649890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilization of the <i>Dasypyrum</i> genus for genetic improvement of wheat.","authors":"Jie Zhang, Qian Chen, Fan Yang, Ying Wang, Jun Xiao, Hongxia Ding, Qiang Ma, Qian Deng, Yun Jiang","doi":"10.1007/s11032-024-01512-6","DOIUrl":"10.1007/s11032-024-01512-6","url":null,"abstract":"<p><p>The <i>Dasypyrum</i> genus species are found predominantly in the Mediterranean region. They possess an array of agronomically essential traits, such as resistance to biotic and abiotic stresses, high protein content, and better grain quality, and are thus a valuable genetic resources for wheat improvement. In recent decades, there has been significant progress in the development of wheat-<i>Dasypyrum</i> genetic stocks, leading to the successful transfer of beneficial genes from <i>Dasypyrum</i> into cultivated wheat. Notably, the chromosome-scale genome assembly of <i>Dasypyrum villosum</i> was preliminarily completed in 2023, laying the groundwork for functional genomics research and wheat-<i>Dasypyrum</i> introgression breeding. This article aims to provide a concise overview of the relationships between different species belonging to the <i>Dasypyrum</i> genus, the development of wheat-<i>Dasypyrum</i> genetic stocks, the desirable genes derived from <i>Dasypyrum</i>, and the molecular and cytogenetic markers that could be used to identify <i>Dasypyrum</i> chromatins. These insights can assist wheat breeders in utilizing the <i>Dasypyrum</i> genus in future wheat breeding endeavors.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 12","pages":"82"},"PeriodicalIF":2.6,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11646256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142837752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping of dwarfing gene and identification of mutant allele on plant height in wheat.","authors":"Xiaomei Xie, Yang Zhang, Le Xu, Hongchun Xiong, Yongdun Xie, Linshu Zhao, Jiayu Gu, Huiyuan Li, Jinfeng Zhang, Yuping Ding, Shirong Zhao, Huijun Guo, Luxiang Liu","doi":"10.1007/s11032-024-01515-3","DOIUrl":"10.1007/s11032-024-01515-3","url":null,"abstract":"<p><p>Plant height is one of the most critical factors influencing wheat plant architecture, and the application of Green Revolution genes has led to a reduction in plant height and an increase in yield. Discovering new dwarfing genes and alleles can contribute to enhance the genetic diversity of wheat. Here we obtained an EMS induced dwarf wheat mutant <i>je0166</i> with increased grain weight, which exhibited a reduction in plant height ranging from 46.47% to 49.40%, and its cell length was shorter. The mutant <i>je0166</i> was sensitive to exogenous gibberellin, but its sensitivity was lower than that of its wild type. Genetic analysis on plant height and gene mapping located the target region to a 4.07 cM interval on chr. 4AL. Within this interval, we identified a co-segregated mutation in <i>Rht-A1h</i>, which is a novel allele of the Green Revolution gene <i>Rht-A1</i>. We also found large fragment inversions in the genetic map of the mutant. The novel allele diversifies natural allelic variations and could be utilized in future wheat improvement. Furthermore, we demonstrated that chemical mutagen treatment led to large fragment inversion.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01515-3.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 11","pages":"79"},"PeriodicalIF":2.6,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome wide association study and transcriptome analysis identify candidate genes regulating wheat coleoptile length.","authors":"Yihan Men, Shan Lu, Ling Li, Chenran Wu, Nannan Sun, Yanju Huang, Tauqeer Ahmad Yasir, Yang Yang, Changhai Wang, Xuefei Gao, Huailong Lin, Lyudmila Zotova, Dauren Serikbay, Yangbin Liu, Yongan Yin, Chaowu Zeng, Yin-Gang Hu, Jianjiang Li, Liang Chen","doi":"10.1007/s11032-024-01520-6","DOIUrl":"10.1007/s11032-024-01520-6","url":null,"abstract":"<p><p>Coleoptile length, in wheat, is a significant agronomic trait impacting yield by facilitating the successful establishment of seedlings. In arid regions, varieties possessing longer coleoptile can evade harsh conditions by deep sowing, paving the way for improved yield. However, the study of genes involved in coleoptile development is insufficient. In this study, a high-density 660 K SNP array was used for genome-wide association study (GWAS) on coleoptile length in 150 wheat varieties. The findings revealed the detection of 353 significantly associated SNPs across all environments. The integration of linkage disequilibrium analysis and haplotype analysis mined 23 core QTLs capable responsible for the stable regulating coleoptile length in wheat. In wheat varieties characterized by extended coleoptile length, 6,600, 11,524, and 6,059 genes were found to be differentially expressed at three distinct developmental stages within the coleoptile, respectively. Through GWAS, gene expression levels, and functional annotation, we concluded the identification of two candidate genes (<i>TraesCS2B02G423500</i>, <i>TraesCS2B02G449200</i>) regulating wheat coleoptile length. By employing WGCNA and protein interactions prediction, discovered that the 19 genes were found to interact with candidate genes and participate in plant hormone metabolism and signaling, cell elongation or proliferation, which collectively contributing to coleoptile elongation. Additionally, two KASP markers were developed which can be used in breeding. These results offer a basis for understanding the genetic regulatory network responsible for wheat coleoptile length formation. The QTLs and candidate genes identified in this study can be further utilized for genetic improvement of wheat coleoptile length.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01520-6.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 11","pages":"78"},"PeriodicalIF":2.6,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11561208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent progress in the understanding of Citrus Huanglongbing: from the perspective of pathogen and citrus host.","authors":"Kun Yang, Bin Hu, Wang Zhang, Tao Yuan, Yuantao Xu","doi":"10.1007/s11032-024-01517-1","DOIUrl":"10.1007/s11032-024-01517-1","url":null,"abstract":"<p><p>Citrus Huanglongbing (HLB) is a devastating disease spread by citrus psyllid, causing severe losses to the global citrus industry. The transmission of HLB is mainly influenced by both the pathogen and the citrus psyllid. The unculturable nature of the HLB bacteria (<i>Candidatus</i> Liberibacter asiaticus, <i>C</i>Las) and the susceptibility of all commercial citrus varieties made it extremely difficult to study the mechanisms of resistance and susceptibility. In recent years, new progress has been made in understanding the virulence factors of <i>C</i>Las as well as the defense strategies of citrus host against the attack of <i>C</i>Las. This paper reviews the recent advances in the pathogenic mechanisms of <i>C</i>Las, the screening of agents targeting the <i>C</i>Las, including antimicrobial peptides, metabolites and chemicals, the citrus host defense response to <i>C</i>Las, and strategies to enhance citrus defense. Future challenges that need to be addressed are also discussed.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 11","pages":"77"},"PeriodicalIF":2.6,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving cabbage resistance to <i>Sclerotinia sclerotiorum</i> via crosses with <i>Brassica incana</i>.","authors":"Qinfei Li, Jiaqin Yang, Xiaoyun Liu, Jiabing Wu, Ao Peng, Jun Si, Xuesong Ren, Jiaqin Mei, Wei Qian, Honghao Lv, Zujun Tang, Hongyuan Song","doi":"10.1007/s11032-024-01513-5","DOIUrl":"https://doi.org/10.1007/s11032-024-01513-5","url":null,"abstract":"<p><p>Cabbage is a widely cultivated leafy vegetable, but head rot disease caused by the fungus <i>Sclerotina sclerotiorum</i> can seriously reduce its yield and quality. There are currently not any cabbage varieties that are completely immune to the disease, but its wild relative <i>Brassica incana</i> is very resistant. In this study, cabbage resistance was improved by backcrossing a highly resistant <i>B. incana</i> accession (C01) with a susceptible cabbage cultivar (F416). Although C01 lacks a leafy head formation, highly resistant plants appeared in the fourth backcrossing generation (BC₄F₁) that had a similar leafy head to F416. The individuals with strong resistance were purified by self-pollination. Inbred lines that maintained a relatively stable resistance at BC₄F₃ were developed and had significantly higher resistance to <i>S. sclerotiorum</i> than F416. In addition, hybrids created from a cross between of BC₄F₃ and E2 had higher resistances to <i>S. sclerotiorum</i> and similar agronomic characteristics to Xiyuan 4. The results demonstrated that new F416 lines that are resistant to <i>S. sclerotiorum</i> can be developed, and that these lines could be used to create new cabbage varieties with superior head rot resistance.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01513-5.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 11","pages":"76"},"PeriodicalIF":2.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11538228/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142605518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}