Molecular Breeding最新文献

{"title":"Chengdan 3601, a new maize variety bred for grain and silage, is suitable for mechanized harvesting.","authors":"Yonghui Zhu, Lin Yang, Junpin Yang, Yan Li, Zhitao Liu, Zenghao Liang, Luchang Deng, Jie Chen, Jun Tan, Haitao Tang, Yuanyuan He, Wenzhu He","doi":"10.1007/s11032-025-01617-6","DOIUrl":"10.1007/s11032-025-01617-6","url":null,"abstract":"","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 11","pages":"91"},"PeriodicalIF":3.0,"publicationDate":"2025-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12596230/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145489351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic dissection of plant height in spring wheat from Pakistan using genome-wide SNPs and allele-specific <i>Rht</i> markers.","authors":"Hafiz Muhammad Suleman, Humaira Qayyum, Sana Ur Rehman, Khawar Majeed, Misbah Mukhtar, Saima Zulfiqar, Zahid Mahmood, Abdul Aziz, Muhammad Fayyaz, Ambreen Mehvish, Shuanghe Cao, Awais Rasheed, Zhonghu He","doi":"10.1007/s11032-025-01600-1","DOIUrl":"10.1007/s11032-025-01600-1","url":null,"abstract":"<p><p>Plant architecture and yield potential of wheat are significantly influenced by plant height (PH). In the present study, a diversity panel consisting of 199 Pakistani wheat cultivars was evaluated for PH in three environments, and a genome-wide association study (GWAS) was conducted to identify loci associated with reduced height. GWAS identified 19 loci associated with reduced height, of which 12 were consistently identified in all environments. Allelic variations were analyzed in the diversity panel for five <i>Rht</i> genes, including <i>Rht-B1</i>, <i>Rht-D1</i>, <i>Rht13</i>, <i>Rht25</i>, and <i>Rht26</i>, using diagnostic KASP markers. Furthermore, a KASP marker was developed to identify the dwarfing allele <i>Rht-B1p</i> in wheat. The GA-insensitive dwarfing allele <i>Rht-B1b</i> allelic frequency was pre-dominant (69.6%), followed by the GA-sensitive <i>Rht26</i> mutant allele (58.5%). Five dwarfing alleles of <i>Rht25</i>, including <i>Rht25c</i>, <i>Rht25d</i>, and <i>Rht25e</i> were rarely present in the cultivars, with frequencies of 1.5%, 1%, and 0.5%, respectively. The use of alternate dwarfing alleles to reduce PH can increase the genetic base of wheat cultivars by reducing selection pressure on the <i>Rht-B1b/Rht-D1b</i> haplotype and can lead to the development of wheat cultivars with improved characteristics such as reduced lodging risk, increased resource allocation to grain, improved harvest efficiency, enhanced crop stability, and adaptability.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01600-1.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 11","pages":"90"},"PeriodicalIF":3.0,"publicationDate":"2025-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12595168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145482579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and QTL analysis of stripe rust resistance in the common wheat cultivar Gaoyuan813.","authors":"Kanghui Zeng, Yuqin Li, Liuyang Shang, Yanling Hu, Zhenzhen Wei, Qiang Zhou, Lianquan Zhang, Dengcai Liu, Bo Zhang, Lin Huang","doi":"10.1007/s11032-025-01616-7","DOIUrl":"10.1007/s11032-025-01616-7","url":null,"abstract":"<p><p>Stripe rust, caused by <i>Puccinia striiformis</i> f. sp. <i>tritici</i> (<i>Pst</i>), is a major threat to global wheat production. Developing resistant wheat varieties is a crucial objective in wheat breeding programs. The Chinese common wheat cultivar Gaoyuan813 (GY813) has exhibited excellent resistance to <i>Pst</i> under field conditions for several years. The objective of this study was to identify quantitative trait loci (QTLs) associated with stripe rust resistance using a recombinant inbred line (RIL) population derived from a cross between Gaoyuan813 and the susceptible variety Fukuho. The RILs were evaluated for stripe rust resistance in four field environments with a mixture of Chinese prevalent <i>Pst</i> races (CYR32, CYR33, CYR34, Zhong4, and HY46) and in a growth chamber with race CYR34 and genotyped using the Wheat55K single nucleotide polymorphism array. Five QTLs for stripe rust resistances were mapped to chromosomes 1BL (2), 2AS (2), and 7DS (1), explaining 4.37%-25.44% of the phenotypic variance. <i>QYrsicau-2AS.1</i> and <i>QYrsicau-7DS</i> were stably detected across all field environments, whereas <i>QYrsicau-1BL.2</i> was only detected in the growth chamber test. <i>QYrsicau-1BL.1</i> and <i>QYrsicau-7DS</i> may correspond to the known resistance genes <i>Yr29</i> and <i>Yr18</i>, respectively, while <i>QYrsicau-1BL.2</i> and <i>QYrsicau-2AS.2</i> are likely novel. Additive effects were observed for the combination of <i>QYrsicau-1BL.1</i>, <i>QYrsicau-2AS.1</i>, and <i>QYrsicau-7DS</i>. KASP markers linked to <i>QYrsicau-1BL.2</i> (<i>KASP_AX-109878201</i>) and <i>QYrsicau-2AS.1</i> (<i>KASP_AX-110981112</i>) were developed and validated to facilitate the breeding use of genes for wheat improvement.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01616-7.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 11","pages":"89"},"PeriodicalIF":3.0,"publicationDate":"2025-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12589750/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145482591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperspectral imaging of grains uncovers the genetic architecture of nitrogen response of development in bread wheat.","authors":"Qiang Liu, Yinyin Zhang, Jiawei Shi, Wanneng Yang, Hui Feng, Weijuan Hu","doi":"10.1007/s11032-025-01609-6","DOIUrl":"10.1007/s11032-025-01609-6","url":null,"abstract":"<p><p>Unraveling the genetic architecture of nitrogen response of development is critical for improving wheat productivity while reducing nitrogen inputs. In this study, hyperspectral imaging (HSI) was applied to wheat grains obtained from nitrogen-deficient and normal conditions, combined with genome-wide association studies (GWAS), to investigate the nitrogen response of development in a diverse wheat panel. The 1,792 i-traits were acquired via hyperspectral imaging system, which reflect detailed phenotypic assessments of wheat development, capturing subtle variations in nitrogen response. A total of 3,556 significant loci and 3,648 candidate genes were identified. Key candidate genes involved in nitrogen uptake and utilization were identified by integrating agronomic traits with i-traits, including <i>TaARE1-7A, TaPTR9-7B, TaNAR2.1,</i> and <i>Rht-B1</i>. This approach underscores the potential of combining HSI on grains with GWAS to dissect complex traits like nitrogen response, offering valuable genetic insights for breeding nitrogen-efficient wheat varieties and enhancing sustainability in crop production.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01609-6.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 11","pages":"88"},"PeriodicalIF":3.6,"publicationDate":"2025-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12583357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145452135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide association study of seven agronomy traits under drought-stressed and well-watered in maize.","authors":"Zhibo Qu, Ning Zhang, Dandan Liu, Haoxiang Yang, Ziran Zhang, Ningning Wei, Wanchao Zhu, Jiquan Xue, Shutu Xu","doi":"10.1007/s11032-025-01599-5","DOIUrl":"10.1007/s11032-025-01599-5","url":null,"abstract":"<p><p>Drought is one of the main factors restricting the increase of maize yield. Many genes respond to drought at seedlings have been cloned but less were effective in field. So, more work of understanding the genetic basis of drought response in field experiment need to be done due to its complexity. Herein, we constructed an association panel to carry on genomic wide association mapping for seven important traits under well-watered at whole period and drought at flowering stage. Then, 117 SNPs were identified, 50 SNPs of which were co-located among these traits or treatments or environments, including 50 SNPs identified under drought and 67 SNPs under well-watered. After merging the co-located SNPs, 90 SNPs were obtained. Combining the RNA-seq data of maize inbred line B73 under drought stressed from the public database, 31 differential expressed genes around the associated SNP were considered as drought responsive genes. Through protein interaction analysis and Gene Ontology enrichment analysis, it was shown that these genes are involved in regulating biological processes such as the tricarboxylic acid cycle, glycolysis, cell mitosis, and flowering signaling. And as the aggregation of related favorable allele genes improves the drought tolerance of materials. These results provide some candidate genes for in-depth analyzing the drought resistance mechanism when the drought happened at flowering stage during field experiment.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01599-5.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 11","pages":"87"},"PeriodicalIF":3.6,"publicationDate":"2025-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12559503/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145401144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a common major quantitative trait locus controlling onion bulb weight and development of molecular markers for bulb weight selection.","authors":"Daisuke Sekine, Satoshi Oku, Utako Yamanouchi, Tatsumi Mizubayashi, Tsuyu Ando, Satoko Hiura, Hikaru Tsukazaki","doi":"10.1007/s11032-025-01614-9","DOIUrl":"10.1007/s11032-025-01614-9","url":null,"abstract":"<p><p>Bulb weight, crucial in onion breeding, is difficult to improve because of its low heritability and environmental variability, limiting phenotype-based selection. Previous studies have identified quantitative trait loci (QTL) for bulb shape; however, no significant QTLs have been detected for bulb weight. This study aimed to develop DNA markers for bulb weight selection through genetic analysis. A breeding line was evaluated in four environments (576 plants), each genotyped using 157 markers. Markers significantly associated with bulb weight were detected on chr8 using a genome-wide association study. An F2 population (245 plants) with a genetic background distinct from that of the breeding line was evaluated, and QTL for bulb weight were detected by composite interval mapping using 348 genome-wide marker genotypes. The position of a major QTL was identical to those of the associated markers detected in the breeding line, suggesting that the QTL is common and stable, regardless of genetic background. Three indel markers were designed near the QTL peak, and their combinations were used for haplotype definition. Eight plants per genetic resource line and commercial F1 cultivar were genotyped, and proportions of haplotypes within each line/cultivar were summarized. Almost all genetic resource lines contained at least two haplotypes, and haplotype pairs were unfixed in several F1 cultivars. Haplotype selection and fixation contribute to bulb weight stability and uniformity, making these DNA markers useful for efficient breeding. This study provides the first stable QTL for bulb weight, offering genetic insights that could facilitate marker-assisted selection and improve onion breeding efficiency.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01614-9.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 11","pages":"86"},"PeriodicalIF":3.6,"publicationDate":"2025-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12553687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145378007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide association mapping for resistance against Septoria nodorum blotch and tan spot in a diverse wheat panel.","authors":"Dehua Wang, Xinyao He, Zhiying Deng, Matthew Reynolds, Susanne Dreisigacker, Pawan K Singh","doi":"10.1007/s11032-025-01602-z","DOIUrl":"10.1007/s11032-025-01602-z","url":null,"abstract":"<p><p>Septoria nodorum blotch (SNB) and tan spot (TS) are two globally significant foliar diseases affecting wheat, causing substantial reductions in both yield and grain quality. In this study, genome-wide association study (GWAS) was conducted using an elite diversity panel comprising 150 lines to identify genetic loci associated with resistance to SNB and TS. Resistance was evaluated in greenhouse experiments at the seedling stage, with two replicates for each disease. For SNB, the majority of lines demonstrated good level of resistance, with 53% rated as resistant or moderately resistant (R/MR). Similarly, for TS, 60% of the lines exhibited R/MR resistance. Some lines exhibited high resistance to both SNB and TS. The panel was genotyped with the Illumina Infinium 25 K BeadChip. GWAS revealed several significant marker-trait associations on chromosome 5B associated with SNB resistance, all of which were located in the vicinity of the <i>Tsn1</i> gene, suggesting its important role in conferring SNB susceptibility within this population. In addition, two quantitative trait loci (QTL) on chromosomes 2AL and 7AS were identified. For TS, significant markers were primarily found within a 20 Mb region on the long arm of chromosome 7B, with phenotypic variation explained ranging from 8.34% to 12.31%. Additional TS QTL with minor effects were identified on chromosomes 3A, 5A, 7A, and 7D. These resistant lines and identification of markers for SNB and TS resistance hold potential for use in wheat breeding programs aimed at improving resistance to the two diseases.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01602-z.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 11","pages":"85"},"PeriodicalIF":3.6,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12549505/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145378066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An artificial inoculation method for evaluating rice resistance to RBSDV.","authors":"Quanguang Sun, Hongliang Zhang, Jun He, Qi Li, Ling Jiang, Yunlong Wang, Yongsheng Wang, Yuqiang Liu, Jianmin Wan","doi":"10.1007/s11032-025-01612-x","DOIUrl":"10.1007/s11032-025-01612-x","url":null,"abstract":"<p><p>Rice black-streaked dwarf virus (RBSDV) disease, caused by rice black-streaked dwarf virus and transmitted by the small brown planthopper (<i>Laodelphax striatellus</i> Fallén, SBPH), is a devastating viral disease severely impacting global rice production. The cultivation and promotion of resistant varieties is considered the most economical and effective control strategy. However, effectiveness of field-based resistance identification is hindered by environmental variability, seasonal limitations of SBPH outbreaks, and inconsistent virus pressure, leading to unreliable results. Therefore, we developed an efficient and reliable artificial inoculation system for RBSDV-resistant rice materials screening under controllable laboratory conditions. This system encompasses optimized procedures for material preparation, inoculation methodology, disease phenotyping and resistance evaluation. We directly inoculated 1st-2nd instar SBPH nymphs onto the RBSDV infected plants and obtained nymphs with high viruliferous rates. Then, to generate a stable laboratory virus source, these viruliferous nymphs were used to inoculate highly susceptible rice seedlings (e.g., Suyunuo). Subsequent inoculation cycles using these viruliferous rice seedlings consistently produced SBPH populations with high viruliferous rates (average about 42.4%). This system enables continuous, year-round resistance evaluation, breaking through the limitation of environmental dependence in field identification. This method will significantly accelerate the breeding of RBSDV disease resistant rice varieties.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01612-x.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 11","pages":"84"},"PeriodicalIF":3.6,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12549438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145378027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and application of functional markers based on the <i>CmAPRR2</i> gene for peel color improvement in Muskmelon (<i>Cucumis melo</i>).","authors":"Rong Fan, Meihua Li, Yongbing Zhang, Bin Liu, Xuejun Zhang, Chaohong Deng, Changchun Fang, Wei Liu, Yong Yang","doi":"10.1007/s11032-025-01604-x","DOIUrl":"10.1007/s11032-025-01604-x","url":null,"abstract":"<p><p>Peel is a crucial determinant of consumer preference and market value in melons, significantly influencing commercial viability. To overcome limitations in conventional breeding for melon peel color improvement, we used 'VZX' (green peel) and 'HP' (yellow peel) as parental materials to construct an F₂ (<i>n</i> = 414) and RIL (<i>n</i> = 116) populations for genetic dissection and mapping. Genetic analysis revealed that green peel color is a dominant trait controlled by a single gene. Using phenotypic data and whole-genome resequencing of the RIL population, we constructed a high-density genetic landscape and identified a major-effect quantitative trait locus (QTL) spanning 77 kb (499.6-576.5 kb on chromosome 4), with a LOD score of 14.85, explaining 41.76% of peel color phenotypic variation. Bulked segregant analysis (BSA) confirmed mapping accuracy. Within this interval, 13 candidate genes were annotated via Gene annotation analysis, including <i>EVM0008709</i>, which encodes the <i>APRR2</i> transcription factor previously associated with chlorophyll metabolism and pigment regulation in melon, watermelon, and other crops. Three foreground selection markers (RC1, RC2, and RC3) were developed based on <i>EVM0008709</i> and demonstrated 100% validation accuracy in both the F₂ and RIL populations. Additionally, leveraging resequencing data from 'VZX' and 'HP', we designed 204 kompetitive allele-specific PCR (KASP) markers, with 141 exhibiting robust genotyping performance for background selection. Using marker-assisted backcrossing (MABC) with 'HP' as the donor and 'VZX' as the recipient, we developed a novel yellow peel germplasm, 'YVZX', which retains the superior flesh quality of 'VZX'. This study establishes an integrated germplasm innovation pipeline spanning from gene mapping to marker development, and molecular breeding, thereby providing a reproducible technical framework for enhancement of melon quality traits.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01604-x.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 11","pages":"83"},"PeriodicalIF":3.6,"publicationDate":"2025-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12537625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145346154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"C13MP, a method using C13 isotope labeling to measure plant photosynthetic rate precisely.","authors":"Qing Xiong, Ruijie Mao, Yizhe Lu, Yanwen Wang, Zhengchang Wang, Zuxin Zhang, Zhihong Li, Jie Liu, Lei Liu","doi":"10.1007/s11032-025-01601-0","DOIUrl":"10.1007/s11032-025-01601-0","url":null,"abstract":"<p><p>Photosynthesis is the cornerstone of life on earth. Precisely evaluating the photosynthetic rate is critical to uncovering its underlying mechanisms. Numerous methods and tools have been developed to assess plant photosynthetic rate. However, these methods typically measure the carbon assimilation rate indirectly during photosynthesis, highlighting the need for a new method to determine plant photosynthetic rate precisely. This study developed an easy and reliable method using ¹³C isotope-labeled CO₂ and isotope element spectrometer technology to measure plant photosynthetic rate (C13MP). C13MP can precisely quantify the abundance of assimilated carbon by photosynthesis in plants. C13MP can also evaluate the carbon flow from leaves to the other organs by tracking the ¹³C isotope. The photosynthetic rates determined with C13MP were significantly correlated with maize biomass-related and yield-related phenotypes, suggesting the reliability of this method. In conclusion, C13MP can easily, precisely, and reliably evaluate carbon assimilation in various parts of the plant, providing a new method for measuring plant photosynthetic rate.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01601-0.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 11","pages":"82"},"PeriodicalIF":3.6,"publicationDate":"2025-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12537642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145346145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}