Molecular Breeding最新文献

{"title":"A novel functional allele of Ehd3 controls flowering time in rice","authors":"Zhipeng Hong, Yingxiang Liu, Mingliang He, Wenyan Zhou, Jingjing Sui, Xiaojie Tian, Qingjie Guan, Xinglong Yu, Kun Li, Qingyun Bu, Xiufeng Li","doi":"10.1007/s11032-024-01472-x","DOIUrl":"https://doi.org/10.1007/s11032-024-01472-x","url":null,"abstract":"<p>Rice flowering time determines its geographical distribution and yield traits. As a short-day plant, rice can grow in the northern long-day conditions due to the functional mutations of many photosensitive genes. In this study, to identify novel genes or alleles that regulate flowering time in high latitude region, two cultivar, Dongnong 413 (DN413) and Yukimochi (XN) showing extreme early flowering were used for investigation. DN413 is around 4.0 days earlier than XN, and both cultivars can be grown in II (2500 ℃–2700 ℃) to III (2300 ℃–2500 ℃) accumulated temperature zones. We found that the two cultivars shared the same genotype of heading date genes, including <i>Hd1/2/4/5/6/16/17/18</i>, <i>Ehd2</i>, <i>DTH2</i>, <i>SE5</i>, <i>Hd3a</i>. Importantly, a novel <i>Ehd3</i> allele characterized by a A1146C substitution was identified, which results in the E382D substitution, hereafter the 382 position E is defined as Hap_E and the 382 position D is defined as Hap_D. Association analysis showed that Hap_E is earlier flowering than Hap_D. Subsequently, we construct DN413 Hap_D line by three times back-crossing DN413 with XN, and found the heading date of DN413 Hap_D was 1.7–3.5 days later than DN413. Moreover, Hap_E and Hap_D of Ehd3 were transformed into <i>ehd3</i> mutant, respectively, and the <i>Ehd3pro:Ehd3D/ehd3</i> flowered later than that <i>Ehd3pro:Ehd3E/ehd3</i> by around 4.3 days. Furthermore, we showed <i>Ehd3</i> functions as a transcriptional suppressor and the substitution of Asp-382 lost the inhibition activity in protoplasts. Finally, a CAPS marker was developed and used for genotyping and marker assistant breeding. Collectively, we discovered a novel functional allele of <i>Ehd3</i>, which can used as a valuable breeding target.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"192 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140889334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsAAP8 mutation leads to significant improvement in the nutritional quality and appearance of rice grains","authors":"Bo Peng, Qingxi Zhang, Yan Liu, Qiang Zhao, Jinhui Zhao, Zhiguo Zhang, Xiaoyu Sun, Juan Peng, Yanfang Sun, Xiaohua Song, Guiying Guo, Yaqin Huang, Ruihua Pang, Wei Zhou, Quanxiu Wang","doi":"10.1007/s11032-024-01473-w","DOIUrl":"https://doi.org/10.1007/s11032-024-01473-w","url":null,"abstract":"<p>Members of the permease gene family are responsible for important biological functions in the growth and development of rice. Here, we show that <i>OsAAP8</i> is a constitutive expression gene, and its translated protein is localized on the cell membrane. Mutation of the <i>OsAAP8</i> can promote the expression of genes related to protein and amylopectin synthesis, and also promote the enlargement of protein bodies in its endosperm, leading to an increase in the protein, amylopectin, and total amino acid content of grains in <i>OsAAP8</i> mutants. Seeds produced by the <i>OsAAP8</i> mutant were larger, and the chalkiness traits of the <i>OsAAP8</i> mutants were significantly reduced, thereby improving the nutritional quality and appearance of rice grains. The OsAAP8 protein is involved in the transport of various amino acids; <i>OsAAP8</i> mutation significantly enhanced the root absorption of a range of amino acids and might affect the distribution of various amino acids. Therefore, <i>OsAAP8</i> is an important quality trait gene with multiple biological functions, which provides important clues for the molecular design of breeding strategies for developing new high-quality varieties of rice.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"60 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140889253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of two plastid transit peptides for construction of pollen-inactivation system in rice","authors":"Menglong Wang, Xiaoqun Peng, Changjian Wang, Xiaoyan Tang","doi":"10.1007/s11032-024-01471-y","DOIUrl":"https://doi.org/10.1007/s11032-024-01471-y","url":null,"abstract":"<p>Hybrid seed production technology (SPT) is achieved through the utilization of a recessive nuclear male-sterile mutant transformed with a transgenic cassette comprising three essential components: the wild-type gene to restore the fertility of the male-sterile mutant, an α-amylase gene to disrupt transgenic pollen grains, and red fluorescence protein gene <i>DsRed</i> to distinguish the transgenic seeds from the nontransgenic male sterile seeds. In rice, we establish the pollen disruption system by introducing an amyloplast targeting signal peptide (ASP) at the N-terminus of maize α-amylase protein ZM-AA1^ΔSP (ZM-AA1 with the N-terminal signal peptide removed). The ASP facilitates the transport of ZM-AA1^ΔSP protein into amyloplast where it degrades starch, resulting in disruption of the pollen fertility. To obtain such signal peptides for rice, we searched the rice proteins homologous to the defined wheat amyloplast proteins followed by protein–protein interaction network predictions and targeting signal peptides prediction. These analyses enabled the identification of four candidate ASPs in rice, which were designated as ASP1, ASP2, ASP3, and ASP4, respectively. ASP1 and ASP2, when linked with ZM-AA1^ΔSP, exhibited the capability to disrupt transgenic pollen grains, whereas ASP3 and ASP4 did not produce this effect. Interestingly, the localization experiments showed that ASP3 and ASP4 were able to target the proteins into chloroplast. The ASP1 and ASP2 sequences provide valuable tools for genetic engineering of the rice male-sterile system, which will contribute to the hybrid rice breeding and production.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"176 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140810774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of QTLs responsible for culturability, and fine-mapping of QTL qCBT9 related to callus browning derived from Dongxiang common wild rice (Oryza rufipogon Griff.)","authors":"Xin Lou, Jingjing Su, Yuzhu Xiong, Min Chen, Qiaowen Zhang, Yanfang Luan, Chuanqing Sun, Yongcai Fu, Kun Zhang","doi":"10.1007/s11032-024-01470-z","DOIUrl":"https://doi.org/10.1007/s11032-024-01470-z","url":null,"abstract":"<p>Compared to <i>japonica</i>, the lower genetic transformation efficiency of <i>indica</i> is a technical bottleneck for rice molecular breeding. Specifically, callus browning frequently occurs during the culture of the elite <i>indica</i> variety 93-11, leading to poor culturability and lower genetic transformation efficiency. Here, 67 QTLs related to culturability were detected using 97 introgression lines (designated as 9DILs) derived from Dongxiang common wild rice (DXCWR, <i>Oryza rufipogon</i> Griff.) with 93-11 genetic background, explaining 4% ~12% of the phenotypic variations. The QTL <i>qCBT9</i> on chromosome 9 was a primary QTL for reducing callus browning derived from DXCWR. Five 9DILs with light callus browning and high differentiation were screened. We evaluated the callus browning index (CBI) of 100 F₂ population crossed of 93-11 and 9DIL71 and the recombinant plants screened from 3270 individuals. The <i>qCBT9</i> was delimited to a ~148kb region between the markers X16 and X23. RNA-seq analysis of DEGs between 9DIL71 and 93-11 showed three upregulated DEGs (Os09g0526500, Os09g0527900, Os09g0528200,) and three downregulated DEGs (Os09g0526700, Os09g0526800, Os09g0527700) were located in the candidate region of <i>qCBT9.</i> Furthermore, callus browning may be involved in cell senescence and death caused by oxidative stress. The differentiation of <i>indica</i> and <i>japonica</i> in this region suggested that <i>qCBT9</i> was possibly a vital QTL contributed to better culturability of <i>japonica</i>. Our results laid a foundation for further cloning of the gene for reduced callus browning in <i>O</i>. <i>rufipogon</i>, and also provided a new genetic resource and material basis for improving the culturability and genetic transformation efficiency of cultivated rice.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"16 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140810777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Huamoxiang 3, a variety bred for steaming and cooking type of whole grain black rice","authors":"Qinglu Zhang, Qifa Zhang, Jinghua Xiao, Hao Chen, Yanhua Li, Sibin Yu, Yuqing He","doi":"10.1007/s11032-024-01469-6","DOIUrl":"https://doi.org/10.1007/s11032-024-01469-6","url":null,"abstract":"","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"89 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140572674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of miRNA-SSR and target-SSR markers from yield-associate genes and their applicability in the assessment of genetic diversity and association mapping in rice (Oryza sativa L.)","authors":"Bavisetti Hemasai, Dinesh K. Kumbha, Vinodkumar Naik Modem, Srividya K. Gannavarapu, Rupeshkumar R. Bommaka, Shanthipriya Mallapuram, Sreelakshmi Chintala, Muga D. Sreevalli, Eswarayya Ramireddy, Lakshminarayana R. Vemireddy","doi":"10.1007/s11032-024-01462-z","DOIUrl":"https://doi.org/10.1007/s11032-024-01462-z","url":null,"abstract":"<p>The gene-derived functional markers are considered effective to use in marker-assisted breeding and genetic diversity analysis. As of now, no functional markers have been identified from miRNAs regulating yield traits. The miRNAs play a key role as regulators in controlling the candidate genes involved in grain yield improvement in rice. In this study, 13 miRNA-SSR and their target gene SSR markers were mined from 29 yield-responsive miRNA along with their 29 target genes in rice. The validation of these markers showed that four miRNA-SSRs and one target gene SSR markers had shown polymorphism among 120 diverse rice genotypes. The PIC values ranged from 0.25 (OsARF18-SSR) to 0.72 (miR408-SSR, miR172b-SSR, and miR396f-SSR) with an average value of 0.57. These polymorphic markers grouped 120 rice genotypes into 3 main clusters based on the levels of high genetic diversity. These markers also showed significant association with key yield traits. Among all, miR172b-SSR showed a strong association with plant height in two seasons. This investigation suggests that this new class of molecular markers has great potential in the characterization of rice germplasm by genetic diversity and population structure and in marker-assisted breeding for the development of high-yielding varieties.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"22 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140603060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsPGL3A encodes a DYW-type pentatricopeptide repeat protein involved in chloroplast RNA processing and regulated chloroplast development","authors":"Min Xu, Xinying Zhang, Jinzhe Cao, Jiali Liu, Yiyuan He, Qingjie Guan, Xiaojie Tian, Jiaqi Tang, Xiufeng Li, Deyong Ren, Qingyun Bu, Zhenyu Wang","doi":"10.1007/s11032-024-01468-7","DOIUrl":"https://doi.org/10.1007/s11032-024-01468-7","url":null,"abstract":"<p>The chloroplast serves as the primary site of photosynthesis, and its development plays a crucial role in regulating plant growth and morphogenesis. The Pentatricopeptide Repeat Sequence (PPR) proteins constitute a vast protein family that function in the post-transcriptional modification of RNA within plant organelles. In this study, we characterized mutant of rice with pale green leaves (<i>pgl3a</i>). The chlorophyll content of <i>pgl3a</i> at the seedling stage was significantly reduced compared to the wild type (WT). Transmission electron microscopy (TEM) and quantitative PCR analysis revealed that <i>pgl3a</i> exhibited aberrant chloroplast development compared to the wild type (WT), accompanied by significant alterations in gene expression levels associated with chloroplast development and photosynthesis. The Mutmap analysis revealed that a single base deletionin the coding region of <i>Os03g0136700</i> in <i>pgl3a</i>. By employing CRISPR/Cas9 mediated gene editing, two homozygous <i>cr</i>-<i>pgl3a</i> mutants were generated and exhibited a similar phenotype to <i>pgl3a</i>, thereby confirming that <i>Os03g0136700</i> was responsible for <i>pgl3a.</i> Consequently, it was designated as <i>OsPGL3A</i>. <i>OsPGL3A</i> belongs to the DYW-type PPR protein family and is localized in chloroplasts. Furthermore, we demonstrated that the RNA editing efficiency of <i>rps8-182</i> and <i>rpoC2-4106</i>, and the splicing efficiency of <i>ycf3-1</i> were significantly decreased in <i>pgl3a</i> mutants compared to WT. Collectively, these results indicate that <i>OsPGL3A</i> plays a crucial role in chloroplast development by regulating the editing and splicing of chloroplast genes in rice.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"15 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140297564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the powdery mildew resistance locus in wheat breeding line Jimai 809 and its breeding application","authors":"Ya Zhao, Guohao Han, Yanmin Qie, Jianmin Song, Yan Zi, Bei Xiao, Jiaojiao Wang, Zejun Qian, Xiaomei Huang, Ruishan Liu, Jiadong Zhang, Lihong Song, Yuli Jin, Pengtao Ma","doi":"10.1007/s11032-024-01467-8","DOIUrl":"https://doi.org/10.1007/s11032-024-01467-8","url":null,"abstract":"<p>Powdery mildew, caused by <i>Blumeria graminis</i> f. sp. <i>tritici</i> (<i>Bgt</i>), is a severe disease that affects the yield and quality of wheat. Popularization of resistant cultivars in production is the preferred strategy to control this disease. In the present study, the Chinese wheat breeding line Jimai 809 showed excellent agronomic performance and high resistance to powdery mildew at the whole growth stage. To dissect the genetic basis for this resistance, Jimai 809 was crossed with the susceptible wheat cultivar Junda 159 to produce segregation populations. Genetic analysis showed that a single dominant gene, temporarily designated <i>PmJM809</i>, conferred the resistance to different <i>Bgt</i> isolates. <i>PmJM809</i> was then mapped on the chromosome arm 2BL and flanked by the markers <i>CISSR02g-1</i> and <i>CIT02g-13</i> with genetic distances 0.4 and 0.8 cM, respectively, corresponding to a physical interval of 704.12–708.24 Mb. <i>PmJM809</i> differed from the reported <i>Pm</i> genes on chromosome arm 2BL in origin, resistance spectrum, physical position and/or genetic diversity of the mapping interval, also suggesting <i>PmJM809</i> was located on a complex interval with multiple resistance genes. To analyze and screen the candidate gene(s) of <i>PmJM809</i>, six genes related to disease resistance in the candidate interval were evaluated their expression patterns using an additional set of wheat samples and time-course analysis post-inoculation of the <i>Bgt</i> isolate E09. As a result, four genes were speculated as the key candidate or regulatory genes. Considering its comprehensive agronomic traits and resistance findings, <i>PmJM809</i> was expected to be a valuable gene resource in wheat disease resistance breeding. To efficiently transfer <i>PmJM809</i> into different genetic backgrounds, 13 of 19 closely linked markers were confirmed to be suitable for marker-assisted selection. Using these markers, a series of wheat breeding lines with harmonious disease resistance and agronomic performance were selected from the crosses of Jimai 809 and several susceptible cultivars.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"21 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140297669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms underlying the signal perception and transduction during seed germination","authors":"Huibin Xu, Fuxiang Wang, Rebecca Njeri Damari, Xiaofeng Chen, Zhongyuan Lin","doi":"10.1007/s11032-024-01465-w","DOIUrl":"https://doi.org/10.1007/s11032-024-01465-w","url":null,"abstract":"<p>QuerySeed germination is a vital step in the life cycle of a plant, playing a significant role in seedling establishment and crop yield potential. It is also an important factor in the conservation of plant germplasm resources. This complex process is influenced by a myriad of factors, including environmental conditions, the genetic makeup of the seed, and endogenous hormones. The perception of these environmental signals triggers a cascade of intricate signal transduction events that determine whether a seed germinates or remains dormant. Despite considerable progress in uncovering the molecular mechanisms governing these processes, many questions remain unanswered. In this review, we summarize the current progress in the molecular mechanisms underlying the perception of environmental signals and consequent signal transduction during seed germination, and discuss questions that need to be addressed to better understand the process of seed germination and develop novel strategies for germplasm improvement.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"48 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140200358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mapping and candidate gene identification of two major quantitative trait loci associated with silique length in oilseed rape (Brassica napus L.)","authors":"","doi":"10.1007/s11032-024-01464-x","DOIUrl":"https://doi.org/10.1007/s11032-024-01464-x","url":null,"abstract":"<h3>Abstract</h3> <p>Rapeseed is a significant global source of plant oil. Silique size, particularly silique length (SL), impacts rapeseed yield. SL is a typical quantitative trait controlled by multiple genes. In our previous study, we constructed a DH population of 178 families known as the 158A-SGDH population. In this study, through SL QTL mapping, we identified twenty-six QTL for SL across five replicates in two environments. A QTL meta-analysis revealed eight consensus QTL, including two major QTL: <em>cqSL.A02-1</em> (11.32–16.44% of PVE for SL), and <em>cqSL.C06-1</em> (10.90–11.95% of PVE for SL). Based on biparental resequencing data and microcollinearity analysis of target regions in <em>Brassica napus</em> and <em>Arabidopsis</em>, we identified 11 candidate genes at <em>cqSL.A02-1</em> and 6 candidate genes at <em>cqSL.C06-1</em>, which are potentially associated with silique development. Furthermore, transcriptome analysis of silique valves from both parents on the 14th, 21st, and 28th days after pollination (DAP) combined with gene function annotation revealed three significantly differentially expressed genes at <em>cqSL.A02-1</em>, <em>BnaA02G0058500ZS</em>, <em>BnaA02G0060100ZS</em>, and <em>BnaA02G0060900ZS</em>. Only the gene <em>BnaC06G0283800ZS</em> showed significant differences in parental transcription at <em>cqSL.C06-1</em>. Two tightly linked insertion-deletion markers for the <em>cqSL.A02-1</em> and <em>cqSL.C06-1</em> loci were developed. Using these two QTL, we generated four combinations: A02^SGDH284C06^158A, A02^SGDH284C06^SGDH284, A02^158AC06^158A, and A02^158AC06^SGDH284. Subsequent analysis identified an ideal QTL combination, A02^158AC06^SGDH284, which exhibited the longest SL of this type, reaching 6.06 ± 0.10 cm, significantly surpassing the other three combinations. The results will provide the basis for the cloning of SL-related genes of rapeseed, along with the development of functional markers of target genes and the breeding of rapeseed varieties.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"21 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140166884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}