Molecular Breeding最新文献

{"title":"Introgression of tetraploid <i>Thinopyrum elongatum</i> 6EL segments enhances the stripe rust resistance of adult wheat plants.","authors":"Chunyan Zeng, Liangxi Li, Zaimei He, Wei Zhu, Lili Xu, Yiran Cheng, Yi Wang, Jian Zeng, Xing Fan, Lina Sha, Haiqin Zhang, Guoyue Chen, Yonghong Zhou, Dandan Wu, Houyang Kang","doi":"10.1007/s11032-024-01493-6","DOIUrl":"10.1007/s11032-024-01493-6","url":null,"abstract":"<p><p>Preventing the widespread occurrence of stripe rust in wheat largely depends on the identification of new stripe rust resistance genes and the breeding of cultivars with durable resistance. In previous study, we reported 6E of wheat-tetraploid <i>Thinopyrum elongatum</i> 6E (6D) substitution line contains adult-stage stripe rust resistance genes. In this study, three novel wheat-tetraploid <i>Th. elongatum</i> translocation lines were generated from the offspring of a cross between common wheat and the 6E (6D) substitution line. Genomic in situ hybridization (GISH), fluorescence in situ hybridization chromosome painting (FISH painting), repetitive sequential FISH, and 55 K SNP analyses indicated that K227-48, K242-82, and K246-6 contained 42 chromosomes and were 6DL·6ES, 2DL·6EL, and 6DS·6EL translocation lines, respectively. The assessment of stripe rust resistance revealed that K227-48 was susceptible to a mixture of <i>Pst</i> races, whereas the 6EL lines K242-82 and K246-6 were highly resistance to stripe rust at the adult stage. Thus, this resistance was due to the chromosome arm 6EL of tetraploid <i>Th. elongatum</i>. The improved agronomic performance of 6DS·6EL translocation line may be a useful novel germplasm resource for wheat breeding programs. For the application of marker-assisted selection (MAS), 47 simple sequence repeat (SSR) markers were developed, showing specific amplification on the chromosome 6E using the whole-genome sequence of diploid <i>Th. elongatum</i>. The 6DS·6EL translocation line and SSR markers have the potential to be deploy for future stripe rust resistance wheat breeding program.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01493-6.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 8","pages":"55"},"PeriodicalIF":2.6,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11327235/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breaking the association between gametocidal gene(s) and leaf rust resistance gene (<i>LrS2427</i>) in <i>Triticum aestivum</i>-<i>Aegilops speltoides</i> derivative by gamma irradiation.","authors":"R Ragini, Niranjana Murukan, Navpreet Kaur Sekhon, Chetna Chugh, Priyanka Agarwal, Prachi Yadav, Niharika Mallick, Shailendra Kumar Jha, Mir Asif Iquebal, Gitanjali Tandon, Aakriti Verma, Bhupinder Singh, Sherry Rachel Jacob, K Raghunandan, Kumble Vinod Prabhu, Shivmangal Singh Tomar, Vinod","doi":"10.1007/s11032-024-01491-8","DOIUrl":"10.1007/s11032-024-01491-8","url":null,"abstract":"<p><p>Utilization of crop wild relatives of wheat can be very effective in building the genetic diversity to cater to the evolving strains of disease pathogens. <i>Aegilops speltoides</i> is a rich source of rust resistance genes however transferring those to wheat genome can be tedious due to co-transfer and preferential transmission of undesirable genes causing gametocidal activity. Such an unholy association was observed in <i>Triticum aestivum</i>-<i>Ae. speltoides</i> derivative line Sel. 2427 which possess the broad-spectrum leaf rust seedling resistance gene (<i>LrS2427</i>). Molecular analysis based on 35 K wheat breeder's array revealed the maximum percentage of <i>Ae. speltoides</i> genome introgression on homoeologous group 2. In situ hybridization studies revealed the presence of S genome in Sel. 2427, showing six translocations on four chromosomes. Karyotyping using repetitive probe (AAG)₆ revealed that the two chromosomes involved are 2D and 2B. Genic regions causing gametocidal activity were identified by dissecting it into component traits and QTLs on 2D and 2B chromosomes were revealed in case of the trait seed shrivelling index. To break the inadvertent association of <i>LrS2427</i> with gametocidal genes, F₁(Agra Local X Sel. 2427) seeds were irradiated with gamma rays and stable leaf rust resistant mutants lacking gametocidal activity were developed. These mutants showed resistance to different races of leaf rust pathogen and showed superior agronomic performance as well. These mutants could be a great resource in wheat improvement for utilization of the leaf rust resistance gene <i>LrS2427</i> without any yield penalty.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01491-8.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 8","pages":"54"},"PeriodicalIF":2.6,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11322474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141988379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>WRKY75</i> regulates anthocyanin accumulation in juvenile citrus tissues.","authors":"Zhihao Lu, Jiaxian He, Jialing Fu, Yuping Huang, Xia Wang","doi":"10.1007/s11032-024-01490-9","DOIUrl":"10.1007/s11032-024-01490-9","url":null,"abstract":"<p><p>The anthocyanin accumulation in juvenile tissues can enhance the ornamental value, attract pollinators, and help improve abiotic stress. Although transcriptional regulation studies of anthocyanin have been relatively extensive, there are few reports on the mechanism of anthocyanin accumulation in young tissues. This study reveals that many juvenile citrus tissues (flowers, leaves, and pericarp) undergo transient accumulation of anthocyanins, exhibiting a red coloration. Using weighted gene co-expression network analysis (WGCNA) identified <i>CitWRKY75</i> as a candidate gene. After detecting the expression levels of <i>CitWRKY75</i> in various citrus juvenile tissues, the expression trend of <i>CitWRKY75</i> was highly consistent with the red exhibiting and fading. Overexpression of <i>CitWRKY75</i> in tobacco significantly increased the anthocyanin content. LUC and yeast one-hybrid assay demonstrated that <i>CitWRKY75</i> could bind to the promoter of <i>CitRuby1</i>(encoding the key transcription factor promoting anthocyanin accumulation) and promote its expression. Finally, comparing the expression levels of <i>CitWRKY75</i> and <i>CitRuby1</i> in the late development stage of blood orange found that <i>CitWRKY75</i> was not the main regulatory factor for anthocyanin accumulation in the later stage. This study used reverse genetics to identify a transcription factor, CitWRKY75, upstream of <i>CitRuby1</i>, which promotes anthocyanin accumulation in citrus juvenile tissues.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01490-9.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 8","pages":"52"},"PeriodicalIF":2.6,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11315850/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141917129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Map-based cloning reveals <i>Cpgp</i> gene encoding an APRR2 protein to regulate the green fruit peel formation in <i>Cucurbita pepo</i>.","authors":"Kiros Gebretsadik, Wanlu Chen, Ying Duan, Yapei Sun, Yadi He, Qinggui Liao, Changlin Wang, Kailiang Bo","doi":"10.1007/s11032-024-01492-7","DOIUrl":"10.1007/s11032-024-01492-7","url":null,"abstract":"<p><p>Fruit peel color is a major factor that influences fruit quality and customers' demand. However, the molecular mechanisms underlying the green fruit peel color trait of <i>Cucurbita pepo</i> L. remain unknown. Two parental lines, RP16 and RP38, were used to study the fruit peel color trait in <i>C. pepo</i>. The parental line RP16 shows white peel color, whereas RP38 exhibits green peel color. 384 F₂ populations were used to identify the inheritance pattern associated with green fruit and white fruit peel in <i>Cucurbita pepo</i> L. 293 F₂ individuals were white, and 91 F₂ individuals were green, resulting in a ratio of 3:1. Hence, white peel is dominant over the green fruit peel trait, and a single recessive green peel gene (<i>Cpgp</i>) controls the green fruit peel. The fruit chlorophyll (Chll) content decreases as fruit matures in the RP16 line. In contrast, Chll increases during the fruit growing periods on fruit peels of the RP38 line. The BSA-sequence analysis revealed the <i>Cpgp</i> locus on Chr5, within a 2.3 Mb region. Subsequent fine-mapping analysis, using 699 F₂ plants, narrowed down this region to 23.90 kb on the same chromosome. Within this region, two annotated genes, namely <i>Cp4.1LG05g02070</i> and <i>Cp4.1LG05g02060</i>, are present. These genes are predicted to encode a two-component Arabidopsis Pseudo-Response Regulator 2-like protein (APRR2), which may be involved in green pigmentation processes in plants. Consequently, sequence alignment and gene expression analyses at various fruit development stages supported that <i>Cp4.1LG05g02070</i> may be the primary candidate gene responsible for regulating the green fruit peel color trait in <i>Cucurbita pepo</i> L. This study may provide a basis for further study on the basic mechanisms that control the fruit peel colors in <i>Cucurbita spp</i>.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01492-7.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 8","pages":"53"},"PeriodicalIF":2.6,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11315825/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141917158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single- and multiple-trait quantitative trait locus analyses for seed oil and protein contents of soybean populations with advanced breeding line background.","authors":"Tu Huynh, Kyujung Van, M A Rouf Mian, Leah K McHale","doi":"10.1007/s11032-024-01489-2","DOIUrl":"10.1007/s11032-024-01489-2","url":null,"abstract":"<p><p>Soybean seed oil and protein contents are negatively correlated, posing challenges to enhance both traits simultaneously. Previous studies have identified numerous oil and protein QTLs via single-trait QTL analysis. Multiple-trait QTL methods were shown to be superior but have not been applied to seed oil and protein contents. Our study aimed to evaluate the effectiveness of single- and multiple-trait multiple interval mapping (ST-MIM and MT-MIM, respectively) for these traits using three recombinant inbred line populations from advanced breeding line crosses tested in four environments. Using original and simulated data, we found that MT-MIM did not outperform ST-MIM for our traits with high heritability (H² > 0.84). Empirically, MT-MIM confirmed only five out of the seven QTLs detected by ST-MIM, indicating single-trait analysis was sufficient for these traits. All QTLs exerted opposite effects on oil and protein contents with varying protein-to-oil additive effect ratios (-0.4 to -4.8). We calculated the economic impact of the allelic variations via estimated processed values (EPV) using the National Oilseed Processors Association (NOPA) and High Yield + Quality (HY + Q) methods. Oil-increasing alleles had positive effects on both EPV_NOPA and EPV_HY+Q when the protein-to-oil ratio was low (-0.4 to -0.7). However, when the ratio was high (-4.1 to -4.8), oil-increasing alleles increased EPV_NOPA and decreased EPV_HY+Q, which penalizes low protein meal. In conclusion, single-trait QTL analysis is adequately effective for high heritability traits like seed oil and protein contents. Additionally, the populations' elite pedigrees and varying protein-to-oil ratios provide potential lines for further yield assessment and direct integration into breeding programs.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01489-2.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 8","pages":"51"},"PeriodicalIF":2.6,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11306453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"QTL detection and candidate gene identification of qCTB1 for cold tolerance in the Yunnan plateau landrace rice","authors":"Haifeng Guo, Yongmei Guo, Yawen Zeng, Andong Zou, Najeeb Ullah Khan, Yunsong Gu, Jin Li, Xingming Sun, Zhanying Zhang, Hongliang Zhang, Youliang Peng, Huahui Li, Zhigang Wu, Pingrong Yuan, Jinjie Li, Zichao Li","doi":"10.1007/s11032-024-01488-3","DOIUrl":"https://doi.org/10.1007/s11032-024-01488-3","url":null,"abstract":"<p>Cold stress is one of the main abiotic stresses that affects rice growth and production worldwide. Dissection of the genetic basis is important for genetic improvement of cold tolerance in rice. In this study, a new source of cold-tolerant accession from the Yunnan plateau, Lijiangxiaoheigu, was used as the donor parent and crossed with a cold-sensitive cultivar, Deyou17, to develop recombinant inbred lines (RILs) for quantitative trait locus (QTL) analysis for cold tolerance at the early seedling and booting stages in rice. In total, three QTLs for cold tolerance at the early seedling stage on chromosomes 2 and 7, and four QTLs at the booting stage on chromosomes 1, 3, 5, and 7, were identified. Haplotype and linear regression analyses showed that QTL pyramiding based on the additive effect of these favorable loci has good potential for cold tolerance breeding. Effect assessment in the RIL and BC₃F₃ populations demonstrated that <i>qCTB1</i> had a stable effect on cold tolerance at the booting stage in the genetic segregation populations. Under different cold stress conditions, <i>qCTB1</i> was fine-mapped to a 341-kb interval between markers M3 and M4. Through the combination of parental sequence comparison, candidate gene-based association analysis, and tissue and cold-induced expression analyses, eight important candidate genes for <i>qCTB1</i> were identified. This study will provide genetic resources for molecular breeding and gene cloning to improve cold tolerance in rice.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"71 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141782644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A genome-wide association study of panicle blast resistance to Magnaporthe oryzae in rice","authors":"Hu Jinlong, Zhang Yu, Wang Ruizhi, Wang Xiaoyu, Feng Zhiming, Xiong Qiangqiang, Zhou Nianbing, Zhou Yong, Wei Haiyan, Zhang Hongcheng, Zhu Jinyan","doi":"10.1007/s11032-024-01486-5","DOIUrl":"https://doi.org/10.1007/s11032-024-01486-5","url":null,"abstract":"<p>Rice blast, caused by <i>Magnaporthe oryzae</i> (<i>M. oryzae</i>), is one of the most serious diseases worldwide. Developing blast-resistant rice varieties is an effective strategy to control the spread of rice blast and reduce the reliance on chemical pesticides. In this study, 477 sequenced rice germplasms from 48 countries were inoculated and assessed at the booting stage. We found that 23 germplasms exhibited high panicle blast resistance against <i>M. oryzae.</i> Genome-wide association analysis (GWAS) identified 43 quantitative trait loci (QTLs) significantly associated (<i>P</i> &lt; 1.0 × 10⁻⁴) with resistance to rice panicle blast. These QTL intervals encompass four genes (<i>OsAKT1</i>, <i>OsRACK1A</i>, <i>Bsr-k1</i> and <i>Pi25</i>/<i>Pid3</i>) previously reported to contribute to rice blast resistance. We selected QTLs with -Log10 (<i>P</i>-value) greater than 6.0 or those detected in two-year replicates, amounting to 12 QTLs, for further candidate gene analysis. Three blast resistance candidate genes (<i>Os06g0316800</i>, <i>Os06g0320000</i>, <i>Pi25</i>/<i>Pid3</i>) were identified based on significant single nucleotide polymorphisms (SNP) distributions within annotated gene sequences across these 12 QTLs and the differential expression levels among blast-resistant varieties after 72 h of inoculation. <i>Os06g0316800</i> encodes a glycine-rich protein, <i>OsGrp6</i>, an important component of plant cell walls involved in cellular stress responses and signaling. <i>Os06g0320000</i> encodes a protein with unknown function (DUF953), part of the thioredoxin-like family, which is crucial for maintaining reactive oxygen species (ROS) homeostasis in vivo, named as <i>OsTrxl1</i>. Lastly, <i>Pi25</i>/<i>Pid3</i> encodes a disease resistance protein, underscoring its potential importance in plant biology. By analyzing the haplotypes of these three genes, we identified favorable haplotypes for blast resistance, providing valuable genetic resources for future rice blast resistance breeding programs.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"25 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141585453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effective strategies for creating self-compatible B. rapa by introgression of mutated SRK and SCR/SP11 genes from B. napus","authors":"Xueli Zhang, Shuangping Heng, Chunxiu Xiao, Cong Liu, Liping Song, Liguang Tang, Congan He, Bincai Wang, Aihua Wang, Changbin Gao","doi":"10.1007/s11032-024-01487-4","DOIUrl":"https://doi.org/10.1007/s11032-024-01487-4","url":null,"abstract":"","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"1 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141547324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel regulator of wheat tillering <i>LT1</i> identified by using an upgraded BSA method, uni-BSA.","authors":"Yundong Yuan, Bo Lyu, Juan Qi, Xin Liu, Yuanzhi Wang, Pierre Delaplace, Yanfang Du","doi":"10.1007/s11032-024-01484-7","DOIUrl":"10.1007/s11032-024-01484-7","url":null,"abstract":"<p><p>Branching/tillering is a critical process for plant architecture and grain yield. However, Branching is intricately controlled by both endogenous and environmental factors. The underlying mechanisms of tillering in wheat remain poorly understood. In this study, we identified <i>Less Tiller 1</i> (<i>LT1</i>) as a novel regulator of wheat tillering using an enhanced bulked segregant analysis (BSA) method, uni-BSA. This method effectively reduces alignment noise caused by the high repetitive sequence content in the wheat genome. Loss-of-function of <i>LT1</i> results in fewer tillers due to defects in axillary meristem initiation and bud outgrowth. We mapped <i>LT1</i> to a 6 Mb region on the chromosome 2D short arm and validated a nucleotide-binding (NB) domain encoding gene as <i>LT1</i> using CRISPR/Cas9. Furthermore, the lower sucrose concentration in the shoot bases of <i>lt1</i> might result in inadequate bud outgrowth due to disturbances in the sucrose biosynthesis pathways. Co-expression analysis suggests that <i>LT1</i> controls tillering by regulating <i>TaROX/TaLAX1</i>, the ortholog of the <i>Arabidopsis</i> tiller regulator <i>REGULATOR OF AXILLARY MERISTEM FORMATION</i> (<i>ROX</i>) or the rice axillary meristem regulator <i>LAX PANICLE1</i> (<i>LAX1</i>). This study not only offers a novel genetic resource for cultivating optimal plant architecture but also underscores the importance of our innovative BSA method. This uni-BSA method enables the swift and precise identification of pivotal genes associated with significant agronomic traits, thereby hastening gene cloning and crop breeding processes in wheat.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01484-7.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 7","pages":"47"},"PeriodicalIF":2.6,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11199477/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing genetic, racial, and geographic diversity among Ethiopian sorghum landraces and implications for heterotic potential for hybrid sorghum breeding.","authors":"Gezahegn Girma, Alemu Tirfessa, Tamirat Bejiga, Amare Seyoum, Moges Mekonen, Amare Nega, Adane Gebreyohannes, Getachew Ayana, Habte Nida, Tesfaye Mengiste, Gebisa Ejeta, Tesfaye Tesso","doi":"10.1007/s11032-024-01483-8","DOIUrl":"10.1007/s11032-024-01483-8","url":null,"abstract":"<p><p>The wealth of sorghum genetic resources in Africa has not been fully exploited for cultivar development in the continent. Hybrid cultivars developed from locally evolved germplasm are more likely to possess a well-integrated assembly of genes for local adaptation, productivity, quality, as well as for defensive traits and broader stability. A subset of 560 sorghum accessions of known fertility reaction representing the major botanical races and agro-ecologies of Ethiopia were characterized for genetic, agronomic and utilization parameters to lay a foundation for cultivar improvement and parental selection for hybrid breeding. Accessions were genotyped using a genotyping by sequencing (GBS) generating 73,643 SNPs for genetic analysis. Significant genetic variability was observed among accessions with Admixture and Discriminant Analysis of Principal Components where 67% of the accessions fell into K=10 clusters with membership coefficient set to > 0.6. The pattern of aggregation of the accessions partially overlapped with racial category and agro-ecological adaptation. Majority of the non-restorer (B-line) accessions primarily of the bicolor race from the wet highland ecology clustered together away from two clusters of fertility restorer (R-line) accessions. Small members of the B accessions were grouped with the R clusters and in vice-versa while significant numbers of both B and R accessions were spread between the major clusters. Such pattern of diversity along with the complementary agronomic data based information indicate the potential for heterosis providing the foundation for initiating hybrid breeding program based on locally adapted germplasm.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01483-8.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 7","pages":"46"},"PeriodicalIF":2.6,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11190104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141443025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}