BnaC09.tfl1 controls determinate inflorescence trait in Brassica napus.

IF 2.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2024-09-30 eCollection Date: 2024-10-01 DOI:10.1007/s11032-024-01503-7

Xutao Zhao, Lingxiong Zan, Niaofei He, Haidong Liu, Xiaorong Xing, Dezhi Du, Guoyong Tang, Kaixiang Li

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引用次数: 0

Abstract

Determinate inflorescence is indeed a pivotal agricultural characteristic in crops, notably impacting the architecture modification of Brassica napus (AACC, 2n = 38). Previous study identified a crucial gene Bnsdt2 that encodes the transcription factor BnaC09.TFL1 (Terminal Flower 1). Here by two alleles were cloned and sequenced from indeterminate 2982 and determinate 4769, respectively, we found that BnaC09.TFL1 harbors two T/C and G/C non-synonymous mutations in exon 1, and contains sixty-six differences in a 1.9 Kb promoter sequence. Subsequently, BnaC09.TFL1 was introduced into B. napus 571 line by genetic complementation and overexpression, transgenic plants 571^CTO lines and 571^TClines were all restored to the indeterminate inflorescence. Interestingly, after BnaC09.TFL1 was knocked out in 'Westar', transgenic plants Westar^Tcr lines were mutated to determinate inflorescences. Additionally, a NIL-4769 line was constructed to evaluate the effect of BnaC09.TFL1 on agronomic traits of Brassica napus, the results demonstrated that BnaC09.tfl1 reduced the plant height and increased the branch number and branch thousand grain weight of Brassica napus. Finally, we performed RT-qPCR, GUS staining and subcellular localization experiments to analyze the expression pattern of BnaC09.TFL1, the results showed that the expression of BnaC09.TFL1 at shoot apex of NIL-4769 was higher than that of 4769, GUS activity was detected at apical of Arabidopsis thaliana and BnC09.TFL1-GFP was detected in cell membrane, nucleus and cytoplasm. Our findings provide a firm molecular foundation for the study of rapeseed's molecular mechanism of determinate inflorescence formation, as well as theoretical guidance for the application of determinate inflorescence in rapeseed breeding.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-024-01503-7.

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BnaC09.tfl1 控制着甘蓝型油菜的决定性花序性状。

决定性花序确实是农作物的一个关键农业特征，尤其影响到甘蓝型油菜（AACC，2n = 38）的结构改造。先前的研究发现了一个关键基因 Bnsdt2，该基因编码转录因子 BnaC09.TFL1（顶花 1）。我们发现 BnaC09.TFL1 在外显子 1 中存在两个 T/C 和 G/C 非同义突变，在 1.9 Kb 的启动子序列中包含 66 个差异。随后，通过基因互补和过表达将 BnaC09.TFL1 导入油菜 571 株系，转基因植株 571CTO 株系和 571TC 株系均恢复为不定花序。有趣的是，在'Westar'中敲除 BnaC09.TFL1 后，转基因植物 WestarTcr 株系变异为不定花序。此外，我们还构建了一个NIL-4769品系来评估BnaC09.TFL1对甘蓝型油菜农艺性状的影响，结果表明BnaC09.TFL1降低了甘蓝型油菜的株高、增加了分枝数和分枝千粒重。最后，我们通过RT-qPCR、GUS染色和亚细胞定位实验分析了BnaC09.TFL1的表达模式，结果表明BnaC09.TFL1在NIL-4769芽顶的表达量高于4769，在拟南芥顶端检测到GUS活性，在细胞膜、细胞核和细胞质中检测到BnC09.TFL1-GFP。我们的发现为研究油菜确定性花序形成的分子机制提供了坚实的分子基础，也为油菜育种中确定性花序的应用提供了理论指导：在线版本包含补充材料，可查阅 10.1007/s11032-024-01503-7。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.