MdABCI17 acts as a positive regulator to enhance apple resistance to Botryosphaeria dothidea

IF 2.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2024-09-11 DOI:10.1007/s11032-024-01501-9

Ying Xiang, Yu-Wen Zhao, Jing-Jing Wu, Xue Bai, Chu-Kun Wang, Chang-Ning Ma, Quan Sun, Da-Gang Hu

{"title":"MdABCI17 acts as a positive regulator to enhance apple resistance to Botryosphaeria dothidea","authors":"Ying Xiang, Yu-Wen Zhao, Jing-Jing Wu, Xue Bai, Chu-Kun Wang, Chang-Ning Ma, Quan Sun, Da-Gang Hu","doi":"10.1007/s11032-024-01501-9","DOIUrl":null,"url":null,"abstract":"The ATP-binding cassette (ABC) superfamily is involved in numerous complex biological processes. However, the understanding of ABCs in plant pathogen defense, particularly against Botryosphaeria dothidea, remains limited. In this study, we identified MdABCI17 that plays a positive role in apple resistance to B. dothidea. Overexpression of MdABCI17 significantly enhanced the resistance of apple calli and fruits to B. dothidea. Our findings revealed that the jasmonic acid (JA) content and the expression of genes associated with JA biosynthesis and signal transduction were higher in stable MdABCI17-overexpressing apple calli than that of wild-type after inoculation with B. dothidea. Similar results were obtained for apple fruits with transient overexpression of MdABCI17. Our research indicates that MdABCI17 enhances apple resistance to B. dothidea through the JA signaling pathway. We further determined that MdABCI17 plays a crucial role in the apple’s response to JA signaling. Moreover, exogenous methyl jasmonate (MeJA) treatment significantly enhanced the effectiveness of MdABCI17 in boosting apple resistance to B. dothidea. We proposed a positive feedback regulatory loop between MdABCI17-mediated apple resistance to B. dothidea and JA signal. In summary, our study offers new insights into the role of ABC superfamily members in the control of plant disease resistance.","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Breeding","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11032-024-01501-9","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

The ATP-binding cassette (ABC) superfamily is involved in numerous complex biological processes. However, the understanding of ABCs in plant pathogen defense, particularly against Botryosphaeria dothidea, remains limited. In this study, we identified MdABCI17 that plays a positive role in apple resistance to B. dothidea. Overexpression of MdABCI17 significantly enhanced the resistance of apple calli and fruits to B. dothidea. Our findings revealed that the jasmonic acid (JA) content and the expression of genes associated with JA biosynthesis and signal transduction were higher in stable MdABCI17-overexpressing apple calli than that of wild-type after inoculation with B. dothidea. Similar results were obtained for apple fruits with transient overexpression of MdABCI17. Our research indicates that MdABCI17 enhances apple resistance to B. dothidea through the JA signaling pathway. We further determined that MdABCI17 plays a crucial role in the apple’s response to JA signaling. Moreover, exogenous methyl jasmonate (MeJA) treatment significantly enhanced the effectiveness of MdABCI17 in boosting apple resistance to B. dothidea. We proposed a positive feedback regulatory loop between MdABCI17-mediated apple resistance to B. dothidea and JA signal. In summary, our study offers new insights into the role of ABC superfamily members in the control of plant disease resistance.

Abstract Image

查看原文本刊更多论文

MdABCI17 作为一种正调控因子，可增强苹果对多瘤菌的抗性

ATP 结合盒（ABC）超家族参与了许多复杂的生物过程。然而，人们对 ABCs 在植物病原体防御中的作用，特别是对 Botryosphaeria dothidea 的作用的了解仍然有限。在这项研究中，我们发现 MdABCI17 在苹果对 B. dothidea 的抗性中发挥着积极作用。过表达 MdABCI17 能显著增强苹果胼胝体和果实对 B. dothidea 的抗性。我们的研究结果表明，在接种 B. dothidea 后，稳定的 MdABCI17 超表达苹果胼胝体中的茉莉酸（JA）含量以及与 JA 生物合成和信号转导相关的基因的表达量均高于野生型。瞬时过表达 MdABCI17 的苹果果实也得到了类似的结果。我们的研究表明，MdABCI17 通过 JA 信号通路增强了苹果对多刺卷叶螟的抗性。我们进一步确定，MdABCI17 在苹果对 JA 信号的响应中起着至关重要的作用。此外，外源茉莉酸甲酯（MeJA）处理能显著增强 MdABCI17 在提高苹果对多刺卷叶螟抗性方面的作用。我们提出了 MdABCI17 介导的苹果对多刺卷叶蛾的抗性与 JA 信号之间的正反馈调节环路。总之，我们的研究为了解 ABC 超家族成员在植物抗病性调控中的作用提供了新的视角。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.