{"title":"Identification and validation of a novel tiller inhibition locus (<i>tin7</i>) on chromosome 2BL in wheat.","authors":"Shuai Hou, Yuzhou Mou, Haojie Li, Caixia Li, Zhiqiang Wang, Yu Lin, Yueyue Liu, Yaxi Liu","doi":"10.1007/s11032-025-01567-z","DOIUrl":null,"url":null,"abstract":"<p><p>Tiller number is a key determinant of the number of spikes per plant, significantly influencing yield. Here, we identify and characterize a novel tiller inhibition line, N2496. Using an F<sub>2</sub> segregating population derived from crossing N2496 and CN16, we mapped this locus. The F<sub>1</sub> line demonstrated a high number of tillers, while the F<sub>2</sub> population exhibited segregated ratios of 3:1 in tiller number. BSR-Seq analysis indicated that only one locus controls tiller number, located on chromosome 2B (Chr. 2B). This genetic analysis confirmed the presence of a single recessive locus controlling the tiller inhibition trait within this population. Subsequently, we constructed a genetic map on Chr. 2B using a wheat 55 K single nucleotide polymorphism array. By combining recombinant analysis with the genotype and phenotype of the F<sub>2-3</sub> family, we identified and named a major and novel locus, <i>tiller inhibition gene</i> (<i>tin7</i>), mapped within a 2.43 cM interval. The influence of <i>tin7</i> was verified across six different background populations all sharing N2496 as a common parent. Using new recombinant lines from these six populations, we further narrowed down the interval of <i>tin7</i> to a genetic interval of 2.08 cM. Analysis of thousand grain weight and grain-related traits suggests that by regulating tiller number, <i>tin7</i> holds the potential to increase yield in wheat. Our research provides access to a novel tiller number locus and available markers for regulating tiller number, which could be used in developing new cultivars with an optimal number of tillers.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01567-z.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 5","pages":"47"},"PeriodicalIF":2.6000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12045912/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Breeding","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11032-025-01567-z","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
Abstract
Tiller number is a key determinant of the number of spikes per plant, significantly influencing yield. Here, we identify and characterize a novel tiller inhibition line, N2496. Using an F2 segregating population derived from crossing N2496 and CN16, we mapped this locus. The F1 line demonstrated a high number of tillers, while the F2 population exhibited segregated ratios of 3:1 in tiller number. BSR-Seq analysis indicated that only one locus controls tiller number, located on chromosome 2B (Chr. 2B). This genetic analysis confirmed the presence of a single recessive locus controlling the tiller inhibition trait within this population. Subsequently, we constructed a genetic map on Chr. 2B using a wheat 55 K single nucleotide polymorphism array. By combining recombinant analysis with the genotype and phenotype of the F2-3 family, we identified and named a major and novel locus, tiller inhibition gene (tin7), mapped within a 2.43 cM interval. The influence of tin7 was verified across six different background populations all sharing N2496 as a common parent. Using new recombinant lines from these six populations, we further narrowed down the interval of tin7 to a genetic interval of 2.08 cM. Analysis of thousand grain weight and grain-related traits suggests that by regulating tiller number, tin7 holds the potential to increase yield in wheat. Our research provides access to a novel tiller number locus and available markers for regulating tiller number, which could be used in developing new cultivars with an optimal number of tillers.
Supplementary information: The online version contains supplementary material available at 10.1007/s11032-025-01567-z.
期刊介绍：
Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer.
All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others.
Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards.
Molecular Breeding core areas:
Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.
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