{"title":"小麦抗白粉病新基因PmL270在7AL染色体上的精细定位。","authors":"Qianyuan Zhang, Anli Gao, Wanying Sun, Jiale Wang, Qiulian Tang, Xiaobei Chen, Pengtao Ma, Shanying Zhu, Hongjie Li, Huagang He","doi":"10.1007/s11032-025-01574-0","DOIUrl":null,"url":null,"abstract":"<p><p>Wheat (<i>Triticum aestivum</i>) is one of the most important cereal crops, providing essential food and nutrition for humans. Wheat powdery mildew, caused by the biotrophic fungal pathogen <i>Blumeria graminis</i> f. sp. <i>tritici</i> (<i>Bgt</i>), seriously threatens wheat production by reducing yield and quality. Utilizing effective powdery mildew resistance (<i>Pm</i>) genes to develop resistant cultivars is a powerful means for controlling this disease. In this study, we identified a new resistance gene, <i>PmL270</i>, from the wheat line L270. By means of bulked segregant RNA‑Seq (BSR‑Seq) and molecular marker analysis, we fine-mapped <i>PmL270</i> to a 0.1-cM interval on chromosome 7AL, flanked by the markers <i>X7AL07</i> and <i>X7AL09</i>. This interval corresponds to a 630-kb region in the reference genome of Chinese Spring. Comparative analysis showed that <i>PmL270</i> is distinct from other <i>Pm</i> genes previously reported on the same chromosome arm. A co-dominant marker, <i>X7AL08</i>, developed from a candidate <i>NLR</i> gene, co-segregated with <i>PmL270</i> in the mapping population and showed high specificity for this gene. The mapping and development of co-segregation marker will facilitate the cloning of <i>PmL270</i> and contribute to its rapid utilization in wheat resistance breeding.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01574-0.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 6","pages":"48"},"PeriodicalIF":3.0000,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12092929/pdf/","citationCount":"0","resultStr":"{\"title\":\"Fine mapping of <i>PmL270</i>, a new powdery mildew resistance gene on chromosome 7AL in wheat.\",\"authors\":\"Qianyuan Zhang, Anli Gao, Wanying Sun, Jiale Wang, Qiulian Tang, Xiaobei Chen, Pengtao Ma, Shanying Zhu, Hongjie Li, Huagang He\",\"doi\":\"10.1007/s11032-025-01574-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Wheat (<i>Triticum aestivum</i>) is one of the most important cereal crops, providing essential food and nutrition for humans. Wheat powdery mildew, caused by the biotrophic fungal pathogen <i>Blumeria graminis</i> f. sp. <i>tritici</i> (<i>Bgt</i>), seriously threatens wheat production by reducing yield and quality. Utilizing effective powdery mildew resistance (<i>Pm</i>) genes to develop resistant cultivars is a powerful means for controlling this disease. In this study, we identified a new resistance gene, <i>PmL270</i>, from the wheat line L270. By means of bulked segregant RNA‑Seq (BSR‑Seq) and molecular marker analysis, we fine-mapped <i>PmL270</i> to a 0.1-cM interval on chromosome 7AL, flanked by the markers <i>X7AL07</i> and <i>X7AL09</i>. This interval corresponds to a 630-kb region in the reference genome of Chinese Spring. Comparative analysis showed that <i>PmL270</i> is distinct from other <i>Pm</i> genes previously reported on the same chromosome arm. A co-dominant marker, <i>X7AL08</i>, developed from a candidate <i>NLR</i> gene, co-segregated with <i>PmL270</i> in the mapping population and showed high specificity for this gene. The mapping and development of co-segregation marker will facilitate the cloning of <i>PmL270</i> and contribute to its rapid utilization in wheat resistance breeding.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01574-0.</p>\",\"PeriodicalId\":18769,\"journal\":{\"name\":\"Molecular Breeding\",\"volume\":\"45 6\",\"pages\":\"48\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2025-05-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12092929/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular Breeding\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s11032-025-01574-0\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/6/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Breeding","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11032-025-01574-0","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/6/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
Fine mapping of PmL270, a new powdery mildew resistance gene on chromosome 7AL in wheat.
Wheat (Triticum aestivum) is one of the most important cereal crops, providing essential food and nutrition for humans. Wheat powdery mildew, caused by the biotrophic fungal pathogen Blumeria graminis f. sp. tritici (Bgt), seriously threatens wheat production by reducing yield and quality. Utilizing effective powdery mildew resistance (Pm) genes to develop resistant cultivars is a powerful means for controlling this disease. In this study, we identified a new resistance gene, PmL270, from the wheat line L270. By means of bulked segregant RNA‑Seq (BSR‑Seq) and molecular marker analysis, we fine-mapped PmL270 to a 0.1-cM interval on chromosome 7AL, flanked by the markers X7AL07 and X7AL09. This interval corresponds to a 630-kb region in the reference genome of Chinese Spring. Comparative analysis showed that PmL270 is distinct from other Pm genes previously reported on the same chromosome arm. A co-dominant marker, X7AL08, developed from a candidate NLR gene, co-segregated with PmL270 in the mapping population and showed high specificity for this gene. The mapping and development of co-segregation marker will facilitate the cloning of PmL270 and contribute to its rapid utilization in wheat resistance breeding.
Supplementary information: The online version contains supplementary material available at 10.1007/s11032-025-01574-0.
期刊介绍：
Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer.
All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others.
Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards.
Molecular Breeding core areas:
Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.
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