Fine mapping of PmL270, a new powdery mildew resistance gene on chromosome 7AL in wheat.

IF 3 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2025-05-20 eCollection Date: 2025-06-01 DOI:10.1007/s11032-025-01574-0

Qianyuan Zhang, Anli Gao, Wanying Sun, Jiale Wang, Qiulian Tang, Xiaobei Chen, Pengtao Ma, Shanying Zhu, Hongjie Li, Huagang He

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引用次数: 0

Abstract

Wheat (Triticum aestivum) is one of the most important cereal crops, providing essential food and nutrition for humans. Wheat powdery mildew, caused by the biotrophic fungal pathogen Blumeria graminis f. sp. tritici (Bgt), seriously threatens wheat production by reducing yield and quality. Utilizing effective powdery mildew resistance (Pm) genes to develop resistant cultivars is a powerful means for controlling this disease. In this study, we identified a new resistance gene, PmL270, from the wheat line L270. By means of bulked segregant RNA‑Seq (BSR‑Seq) and molecular marker analysis, we fine-mapped PmL270 to a 0.1-cM interval on chromosome 7AL, flanked by the markers X7AL07 and X7AL09. This interval corresponds to a 630-kb region in the reference genome of Chinese Spring. Comparative analysis showed that PmL270 is distinct from other Pm genes previously reported on the same chromosome arm. A co-dominant marker, X7AL08, developed from a candidate NLR gene, co-segregated with PmL270 in the mapping population and showed high specificity for this gene. The mapping and development of co-segregation marker will facilitate the cloning of PmL270 and contribute to its rapid utilization in wheat resistance breeding.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-025-01574-0.

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小麦抗白粉病新基因PmL270在7AL染色体上的精细定位。

小麦（Triticum aestivum）是最重要的谷类作物之一，为人类提供必需的食物和营养。小麦白粉病是由生物营养真菌病原菌Blumeria graminis f. sp. tritici （Bgt）引起的小麦白粉病，严重威胁小麦产量和品质。利用有效的白粉病抗性基因培育白粉病抗性品种是防治白粉病的有力手段。本研究从小麦品系L270中鉴定出一个新的抗性基因PmL270。通过体积分离RNA - Seq （BSR - Seq）和分子标记分析，我们将PmL270精细定位到染色体7AL上的0.1 cm区间，两侧是标记X7AL07和X7AL09。该区间对应于中国春参考基因组中一个630-kb的区域。对比分析表明，PmL270与先前报道的同一染色体臂上的其他Pm基因不同。从候选NLR基因发展而来的共显性标记X7AL08，在定位群体中与PmL270共分离，显示出该基因的高特异性。共分离标记的定位和开发将为PmL270的克隆提供便利，并有助于其在小麦抗性育种中的快速利用。补充资料：在线版本提供补充资料，网址为10.1007/s11032-025-01574-0。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.