Structural variation in the GH1 promoter regulates the hull color of rice.

IF 3 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2025-05-29 eCollection Date: 2025-06-01 DOI:10.1007/s11032-025-01569-x

Chengxing Du, Hao Wang, Guangrong Zhong, Xilan Yan, Kehan Di, Longkang Li, Senhai Zhang, Nangu Yang, Yangkai Wang, Weilan Chen, Hua Yuan, Bin Tu, Jiawei Xiong, Zhaohui Zhong, Yuping Wang, Shigui Li, Peng Qin, Bingtian Ma

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引用次数: 0

Abstract

Rice hull color (HC) is crucial for improving the mechanization efficiency of hybrid rice seed production. However, the genetic resources for hull color currently available for practical production are limited, highlighting an urgent need to explore natural variations that can be utilized in breeding. In this study, we conducted a genome-wide association study (GWAS) on the hull color of 301 rice germplasm resources and identified a significant quantitative trait locus (QTL) qHC3.2 on chromosome 3. In this QTL, we identified a 7.3 kb natural structural variation (SV) in the Golden Hull 1 (GH1) promoter region, which suppresses the expression of GH1 and leads to the golden hull phenotype. We have screened seven germplasm resources that contain this natural variation. By introducing GH1™ into U1S, the hull color of U1S™ remains stable and is unaffected by drying time, demonstrating its potential value for breeding applications. Our study provides valuable natural variations and germplasm resources for the mechanized production of hybrid rice.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-025-01569-x.

查看原文本刊更多论文

GH1启动子的结构变异调控稻壳颜色。

稻壳颜色是提高杂交水稻制种机械化效率的关键。然而，目前可用于实际生产的船体颜色遗传资源有限，因此迫切需要探索可用于育种的自然变异。本研究对301份水稻种质资源的壳色进行了全基因组关联分析，在3号染色体上发现了一个重要的数量性状位点qHC3.2。在这个QTL中，我们在金壳1 （Golden Hull 1, GH1）启动子区域发现了7.3 kb的自然结构变异（SV），该变异抑制了GH1的表达并导致了金壳表型。我们筛选了7种含有这种自然变异的种质资源。通过将GH1™引入到U1S中，U1S™的船体颜色保持稳定，不受干燥时间的影响，展示了其在育种应用中的潜在价值。本研究为杂交水稻的机械化生产提供了有价值的自然变异和种质资源。补充资料：在线版本包含补充资料，提供地址为10.1007/s11032-025-01569-x。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.