{"title":"标记辅助对豇豆优良品种C-152的白叶枯病和花叶病毒抗性的导入。","authors":"Hirenallur Chandappa Lohithaswa, Hebse Bhojappa Dinesh, Sidramappa Channappa Talekar, Kannalli Paramashivaiah Viswanatha, Mallana Goudra Mallikarjuna, Muntagodu Shreekanth Sowmya, Thaggihalli Veeranna Krishna, Poonam Singh, Dasannanamalige Siddeshi Ambika","doi":"10.1007/s11032-025-01570-4","DOIUrl":null,"url":null,"abstract":"<p><p>Cowpea is an important grain legume crop and a source of vegetarian protein. C-152, a popular and widely adapted variety of cowpea, became susceptible to major cowpea diseases, viz., bacterial leaf blight (BLB) and cowpea mosaic virus (CpMV). Thus, in the current investigation, we have introgressed BLB and CpMV resistance from V-16 and V-57817, respectively, to the C-152 variety. A marker assisted simultaneous and stepwise backcross breeding scheme was used to recover an improved version of C-152 with resistance to BLB and CpMV diseases. Foreground (CISP markers VuMt401 and VuMt397 for BLB and SSR markers MA15 and MA80 for CpMV) and background selections were practiced using gene-specific and recurrent genome specific (72 markers) polymorphic markers. Two independent BC<sub>2</sub>F<sub>4</sub> lines from each cross possessing <i>blb-1</i> and <i>cowpea mosaic resistance</i> gene with maximum genome recovery of the C-152 were inter-crossed to derive an inter-cross (IC) F<sub>4</sub> population. Among the 10 promising ICF<sub>4</sub> progenies, the line MC 17-2 (KBC-12), showing high yielding with resistance to BLB and CpMV, was selected. The superiority of the cowpea line MC 17-2 was evident in terms of a yield advantage of 8.68 to 28.68%, 9.30-47.00%, 1.10-8.10% over different check varieties in the initial varietal trial, advanced varietal trial (AVT)-I, and AVT-II, respectively. Further, the multi-location evaluation of KBC-12 (MC 17-2) with the check KBC-9 covering zones 5 and 6 of Karnataka reconfirmed the high-yielding potential and stability of KBC-12 across tested environments, as evident from AMMI and GGE biplots. Thus, the promising cowpea line KBC-12 was released for commercial cultivation in zones 5 and 6 in southern India during 2024 and can also be used as a donor (IC652010) of BLB and CpMV resistance. Our current study is one such examples that revealed the power of marker-assisted selection to deliver improved cultivars from lab to farmers' field.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01570-4.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 6","pages":"52"},"PeriodicalIF":3.0000,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12119430/pdf/","citationCount":"0","resultStr":"{\"title\":\"Marker assisted introgression of bacterial leaf blight and cowpea mosaic virus resistance into an elite cowpea cultivar C-152.\",\"authors\":\"Hirenallur Chandappa Lohithaswa, Hebse Bhojappa Dinesh, Sidramappa Channappa Talekar, Kannalli Paramashivaiah Viswanatha, Mallana Goudra Mallikarjuna, Muntagodu Shreekanth Sowmya, Thaggihalli Veeranna Krishna, Poonam Singh, Dasannanamalige Siddeshi Ambika\",\"doi\":\"10.1007/s11032-025-01570-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Cowpea is an important grain legume crop and a source of vegetarian protein. C-152, a popular and widely adapted variety of cowpea, became susceptible to major cowpea diseases, viz., bacterial leaf blight (BLB) and cowpea mosaic virus (CpMV). Thus, in the current investigation, we have introgressed BLB and CpMV resistance from V-16 and V-57817, respectively, to the C-152 variety. A marker assisted simultaneous and stepwise backcross breeding scheme was used to recover an improved version of C-152 with resistance to BLB and CpMV diseases. Foreground (CISP markers VuMt401 and VuMt397 for BLB and SSR markers MA15 and MA80 for CpMV) and background selections were practiced using gene-specific and recurrent genome specific (72 markers) polymorphic markers. Two independent BC<sub>2</sub>F<sub>4</sub> lines from each cross possessing <i>blb-1</i> and <i>cowpea mosaic resistance</i> gene with maximum genome recovery of the C-152 were inter-crossed to derive an inter-cross (IC) F<sub>4</sub> population. Among the 10 promising ICF<sub>4</sub> progenies, the line MC 17-2 (KBC-12), showing high yielding with resistance to BLB and CpMV, was selected. The superiority of the cowpea line MC 17-2 was evident in terms of a yield advantage of 8.68 to 28.68%, 9.30-47.00%, 1.10-8.10% over different check varieties in the initial varietal trial, advanced varietal trial (AVT)-I, and AVT-II, respectively. Further, the multi-location evaluation of KBC-12 (MC 17-2) with the check KBC-9 covering zones 5 and 6 of Karnataka reconfirmed the high-yielding potential and stability of KBC-12 across tested environments, as evident from AMMI and GGE biplots. Thus, the promising cowpea line KBC-12 was released for commercial cultivation in zones 5 and 6 in southern India during 2024 and can also be used as a donor (IC652010) of BLB and CpMV resistance. 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Marker assisted introgression of bacterial leaf blight and cowpea mosaic virus resistance into an elite cowpea cultivar C-152.
Cowpea is an important grain legume crop and a source of vegetarian protein. C-152, a popular and widely adapted variety of cowpea, became susceptible to major cowpea diseases, viz., bacterial leaf blight (BLB) and cowpea mosaic virus (CpMV). Thus, in the current investigation, we have introgressed BLB and CpMV resistance from V-16 and V-57817, respectively, to the C-152 variety. A marker assisted simultaneous and stepwise backcross breeding scheme was used to recover an improved version of C-152 with resistance to BLB and CpMV diseases. Foreground (CISP markers VuMt401 and VuMt397 for BLB and SSR markers MA15 and MA80 for CpMV) and background selections were practiced using gene-specific and recurrent genome specific (72 markers) polymorphic markers. Two independent BC2F4 lines from each cross possessing blb-1 and cowpea mosaic resistance gene with maximum genome recovery of the C-152 were inter-crossed to derive an inter-cross (IC) F4 population. Among the 10 promising ICF4 progenies, the line MC 17-2 (KBC-12), showing high yielding with resistance to BLB and CpMV, was selected. The superiority of the cowpea line MC 17-2 was evident in terms of a yield advantage of 8.68 to 28.68%, 9.30-47.00%, 1.10-8.10% over different check varieties in the initial varietal trial, advanced varietal trial (AVT)-I, and AVT-II, respectively. Further, the multi-location evaluation of KBC-12 (MC 17-2) with the check KBC-9 covering zones 5 and 6 of Karnataka reconfirmed the high-yielding potential and stability of KBC-12 across tested environments, as evident from AMMI and GGE biplots. Thus, the promising cowpea line KBC-12 was released for commercial cultivation in zones 5 and 6 in southern India during 2024 and can also be used as a donor (IC652010) of BLB and CpMV resistance. Our current study is one such examples that revealed the power of marker-assisted selection to deliver improved cultivars from lab to farmers' field.
Supplementary information: The online version contains supplementary material available at 10.1007/s11032-025-01570-4.
期刊介绍：
Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer.
All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others.
Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards.
Molecular Breeding core areas:
Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.
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