Journal of Biophotonics最新文献_第8页

Characterization and classification of pathogenic bacteria using native fluorescence and spectral deconvolution 利用原生荧光和光谱解卷积对病原菌进行表征和分类。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-06-07 DOI: 10.1002/jbio.202300566

Anandh Sundaramoorthy, Ganesan Bharanidharan, Aruna Prakasarao, Singaravelu Ganesan

{"title":"Characterization and classification of pathogenic bacteria using native fluorescence and spectral deconvolution","authors":"Anandh Sundaramoorthy, Ganesan Bharanidharan, Aruna Prakasarao, Singaravelu Ganesan","doi":"10.1002/jbio.202300566","DOIUrl":"10.1002/jbio.202300566","url":null,"abstract":"Identification and classification of pathogenic bacterial strains is of current interest for the early treatment of diseases. In this work, protein fluorescence from eight different pathogenic bacterial strains were characterized using steady state and time resolved fluorescence spectroscopy. The spectral deconvolution method was also employed to decompose the emission contribution from different intrinsic fluorophores and extracted various key parameters, such as intensity, emission maxima, emission line width of the fluorophores, and optical redox ratio. The change in average lifetime values across different bacterial strains exhibits good statistical significance (p ≤ 0.01). The variations in the photophysical characteristics of bacterial strains are due to the different conformational states of the proteins. The stepwise multiple linear discriminate analysis of fluorescence emission spectra at 280 nm excitation across eight different bacterial strains classifies the original groups and cross validated group with 100% and 99.5% accuracy, respectively.","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141285695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An in vitro feasibility study of 355 nm laser atherectomy for the treatment of peripheral atherosclerotic lesions 用于治疗外周动脉粥样硬化病变的 355 nm 激光动脉粥样切除术的体外可行性研究。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-05-13 DOI: 10.1002/jbio.202400110

Hui Wang, Hui Zuo, Dikang Pan, Yihui Cao, Yiqun Zhang, Duan Liu, Lianrui Guo, Jianming Guo

引用次数: 0

Towards non-invasive tissue hydration measurements with optical coherence tomography 利用光学相干断层扫描技术实现无创组织水合测量。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-05-12 DOI: 10.1002/jbio.202300532

Linda B. Neubrand, Ton G. van Leeuwen, Dirk J. Faber

{"title":"Towards non-invasive tissue hydration measurements with optical coherence tomography","authors":"Linda B. Neubrand, Ton G. van Leeuwen, Dirk J. Faber","doi":"10.1002/jbio.202300532","DOIUrl":"10.1002/jbio.202300532","url":null,"abstract":"The attenuation coefficient (<math>\u0000 <msub>\u0000 <mi>μ</mi>\u0000 <mi>OCT</mi>\u0000 </msub></math>) measured by optical coherence tomography (OCT) has been used to determine tissue hydration. Previous dual-wavelength OCT systems could not attain the needed precision, which we attribute to the absence of wavelength-dependent scattering of tissue in the underlying model. Assuming that scattering can be described using two parameters, we propose a triple/quadrupole-OCT system to achieve clinically relevant precision in water volume fraction. In this study, we conduct a quantitative analysis to determine the necessary precision of <math>\u0000 <msub>\u0000 <mi>μ</mi>\u0000 <mi>OCT</mi>\u0000 </msub></math> measurements and compare it with numerical simulation. Our findings emphasize that achieving a clinically relevant assessment of a 2% water fraction requires determining the attenuation coefficient with a remarkable precision of 0.01 <math>\u0000 <mi>m</mi>\u0000 <msup>\u0000 <mi>m</mi>\u0000 <mrow>\u0000 <mo>−</mo>\u0000 <mn>1</mn>\u0000 </mrow>\u0000 </msup></math>. This precision threshold is influenced by the chosen wavelength for attenuation measurement and can be enhanced through the inclusion of a fourth wavelength range.","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202300532","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140912136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Teachability of multispectral optoacoustic tomography 多光谱光声断层扫描的可教性。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-05-08 DOI: 10.1002/jbio.202400106

Yi Li, Janek Gröhl, Briain Haney, Milenko Caranovic, Eva Lorenz-Meyer, Nikolaos Papatheodorou, Julius Kempf, Adrian P. Regensburger, Emmanuel Nedoschill, Adrian Buehler, Gregor Siebenlist, Werner Lang, Michael Uder, Markus F. Neurath, Maximilian Waldner, Ferdinand Knieling, Ulrich Rother

{"title":"Teachability of multispectral optoacoustic tomography","authors":"Yi Li, Janek Gröhl, Briain Haney, Milenko Caranovic, Eva Lorenz-Meyer, Nikolaos Papatheodorou, Julius Kempf, Adrian P. Regensburger, Emmanuel Nedoschill, Adrian Buehler, Gregor Siebenlist, Werner Lang, Michael Uder, Markus F. Neurath, Maximilian Waldner, Ferdinand Knieling, Ulrich Rother","doi":"10.1002/jbio.202400106","DOIUrl":"10.1002/jbio.202400106","url":null,"abstract":"To date, the appropriate training required for the reproducible operation of multispectral optoacoustic tomography (MSOT) is poorly discussed. Therefore, the aim of this study was to assess the teachability of MSOT imaging. Five operators (two experienced and three inexperienced) performed repositioning imaging experiments. The inexperienced received the following introductions: personal supervision, video meeting, or printed introduction. The task was to image the exact same position on the calf muscle for seven times on five volunteers in two rounds of investigations. In the first session, operators used ultrasound guidance during measurements while using only photoacoustic data in the second session. The performance comparison was carried out with full-reference image quality measures to quantitatively assess the difference between repeated scans. The study demonstrates that given a personal supervision and hybrid ultrasound real-time imaging in MSOT measurements, inexperienced operators are able to achieve the same level as experienced operators in terms of repositioning accuracy.","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400106","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140891405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-frequency photoacoustic and ultrasound imaging for skin evaluation: Pilot study for the assessment of a chemical burn 用于皮肤评估的高频光声和超声成像：评估化学烧伤的试点研究。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-05-08 DOI: 10.1002/jbio.202300460

Juliana Benavides-Lara, Amanda P. Siegel, Maria M. Tsoukas, Kamran Avanaki

{"title":"High-frequency photoacoustic and ultrasound imaging for skin evaluation: Pilot study for the assessment of a chemical burn","authors":"Juliana Benavides-Lara, Amanda P. Siegel, Maria M. Tsoukas, Kamran Avanaki","doi":"10.1002/jbio.202300460","DOIUrl":"10.1002/jbio.202300460","url":null,"abstract":"Skin architecture and its underlying vascular structure could be used to assess the health status of skin. A non-invasive, high resolution and deep imaging modality able to visualize skin subcutaneous layers and vasculature structures could be useful for determining and characterizing skin disease and trauma. In this study, a multispectral high-frequency, linear array-based photoacoustic/ultrasound (PAUS) probe is developed and implemented for the imaging of rat skin in vivo. The study seeks to demonstrate the probe capabilities for visualizing the skin and its underlying structures, and for monitoring changes in skin structure and composition during a 5-day course of a chemical burn. We analayze composition of lipids, water, oxy-hemoglobin, and deoxy-hemoglobin (for determination of oxygen saturation) in the skin tissue. The study successfully demonstrated the high-frequency PAUS imaging probe was able to provide 3D images of the rat skin architecture, underlying vasculature structures, and oxygen saturation, water, lipids and total hemoglobin.","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202300460","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140891374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond life: Exploring hemodynamic patterns in postmortem mice brains 超越生命：探索死后小鼠大脑的血液动力学模式。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-05-07 DOI: 10.1002/jbio.202400017

Anton Sdobnov, Vassiliy Tsytsarev, Gennadi Piavchenko, Alexander Bykov, Igor Meglinski

{"title":"Beyond life: Exploring hemodynamic patterns in postmortem mice brains","authors":"Anton Sdobnov, Vassiliy Tsytsarev, Gennadi Piavchenko, Alexander Bykov, Igor Meglinski","doi":"10.1002/jbio.202400017","DOIUrl":"10.1002/jbio.202400017","url":null,"abstract":"We utilize Laser Speckle Contrast Imaging (LSCI) for visualizing cerebral blood flow in mice during and post-cardiac arrest. Analyzing LSCI images, we noted temporal blood flow variations across the brain surface for hours postmortem. Fast Fourier Transform (FFT) analysis depicted blood flow and microcirculation decay post-death. Continuous Wavelet Transform (CWT) identified potential cerebral hemodynamic synchronization patterns. Additionally, non-negative matrix factorization (NMF) with four components segmented LSCI images, revealing structural subcomponent alterations over time. This integrated approach of LSCI, FFT, CWT, and NMF offers a comprehensive tool for studying cerebral blood flow dynamics, metaphorically capturing the ‘end of the tunnel’ experience. Results showed primary postmortem hemodynamic activity in the olfactory bulbs, followed by blood microflow relocations between somatosensory and visual cortical regions via the superior sagittal sinus. This method opens new avenues for exploring these phenomena, potentially linking neuroscientific insights with mysteries surrounding consciousness and perception at life's end.","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140878192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical coherence elastography with osmotically induced strains: Preliminary demonstration for express detection of cartilage degradation 利用渗透诱导应变进行光学相干弹性成像：快速检测软骨退化的初步验证

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-05-04 DOI: 10.1002/jbio.202400016

Yulia M. Alexandrovskaya, Ekaterina M. Kasianenko, Alexander A. Sovetsky, Alexander L. Matveyev, Dmitry A. Atyakshin, Olga I. Patsap, Mikhail A. Ignatiuk, Artem V. Volodkin, Vladimir Y. Zaitsev

{"title":"Optical coherence elastography with osmotically induced strains: Preliminary demonstration for express detection of cartilage degradation","authors":"Yulia M. Alexandrovskaya, Ekaterina M. Kasianenko, Alexander A. Sovetsky, Alexander L. Matveyev, Dmitry A. Atyakshin, Olga I. Patsap, Mikhail A. Ignatiuk, Artem V. Volodkin, Vladimir Y. Zaitsev","doi":"10.1002/jbio.202400016","DOIUrl":"10.1002/jbio.202400016","url":null,"abstract":"Optical coherence elastography (OCE) demonstrated impressive abilities for diagnosing tissue types/states using differences in their biomechanics. Usually, OCE visualizes tissue deformation induced by some additional stimulus (e.g., contact compression or auxiliary elastic-wave excitation). We propose a new variant of OCE with osmotically induced straining (OIS-OCE) and demonstrate its application to assess various stages of proteoglycan content degradation in cartilage. The information-bearing signatures in OIS-OCE are the magnitude and rate of strains caused by the application of osmotically active solutions onto the sample surface. OCE examination of the induced strains does not require special tissue preparation, the osmotic stimulation is highly reproducible, and strains are observed in noncontact mode. Several minutes suffice to obtain a conclusion. These features are promising for intraoperative method usage when express assessment of tissue state is required during surgical operations. The “waterfall” images demonstrate the development of cumulative osmotic strains in control and degraded cartilage samples.","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140826859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial targeted delivery of riboflavin with a controlled corneal iontophoresis delivery system in theranostic-guided UV-A light photo-therapy 在治疗仪引导的紫外线-A 光光疗中使用可控角膜离子透入递送系统定向递送核黄素

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-05-02 DOI: 10.1002/jbio.202400068

Marco Lombardo, Sebastiano Serrao, Giuseppe Massimo Bernava, Giuseppe Lombardo

{"title":"Spatial targeted delivery of riboflavin with a controlled corneal iontophoresis delivery system in theranostic-guided UV-A light photo-therapy","authors":"Marco Lombardo, Sebastiano Serrao, Giuseppe Massimo Bernava, Giuseppe Lombardo","doi":"10.1002/jbio.202400068","DOIUrl":"10.1002/jbio.202400068","url":null,"abstract":"Seven human donor eye globes underwent corneal cross-linking using theranostic UV-A device with accessory corneal iontophoresis system for patterned delivery of a 0.22% riboflavin solution. Theranostic-guided UV-A light illumination assessed riboflavin distribution and treated corneas at 10 mW/cm2 for 9 min with a 5.0-mm beam size. Corneal topography maps were taken at baseline and 2-h post-treatment. Analysis utilized corneal topography elevation data, with results showing controlled riboflavin delivery led to a consistent gradient, with 40% higher levels centrally (248 ± 79 μg/cm3) than peripherally (180 ± 72 μg/cm3 at ±2.5 mm from the center). Theranostic-guided UV-A light irradiation resulted in significant changes in corneal topography, with a decrease in best-fit sphere value (−0.7 ± 0.2 D; p < 0.001) and consistent downward shift in corneal elevation map (−11.7 ± 3.7 μm). The coefficient of variation was 2.5%, indicating high procedure performance in achieving significant and reliable corneal flattening.","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140827088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiwavelength laser diode based portable photoacoustic and ultrasound imaging system for point of care applications 基于多波长激光二极管的便携式光声和超声成像系统，用于医疗点应用

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-05-02 DOI: 10.1002/jbio.202400058

Mahaan Mitra, Abigail Haworth, Prameth Gaddale, Faisal Badran, Nuzhath Lagno, Colette Pameijer, Faisal Aziz, Sri-Rajasekhar Kothapalli

{"title":"Multiwavelength laser diode based portable photoacoustic and ultrasound imaging system for point of care applications","authors":"Mahaan Mitra, Abigail Haworth, Prameth Gaddale, Faisal Badran, Nuzhath Lagno, Colette Pameijer, Faisal Aziz, Sri-Rajasekhar Kothapalli","doi":"10.1002/jbio.202400058","DOIUrl":"10.1002/jbio.202400058","url":null,"abstract":"Vascular diseases are a leading cause of death and disability worldwide. Despite having precursor conditions like peripheral arterial disease (PAD), they are often only diagnosed after the onset of stroke or heart attack. Low-cost, portable, noninvasive, point-of-care (POC), label-free assessment of deep vascular function benefits PAD diagnosis, especially in resource poor settings of the world. Doppler ultrasound-based blood flow measurements can diagnose PAD, albeit with limited sensitivity and specificity. To overcome this, here, we propose the first-of-its-kind dual-modality photoacoustic-and-ultrasound (PAUS) imaging system that integrates a multiwavelength pulsed laser diode (PLD) with a compact ultrasound data acquisition unit. The mesoscopic imaging depth of the portable PLD-PAUS system was validated using tissue phantoms, and its multispectral photoacoustic imaging capabilities were validated using an atherosclerosis-mimicking phantom. Furthermore, we demonstrated high-contrast volumetric in vivo photoacoustic imaging of rodent abdominal vasculature and quantified vessel reactivity due to hypercapnia stimulation. The multiparametric functional and molecular imaging capabilities of the PLD-PAUS system holds promise for POC applications.","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400058","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140826854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An FBG-based optical pressure sensor for the measurement of radial artery pulse pressure 用于测量桡动脉脉压的基于 FBG 的光学压力传感器。

IF 2 3区物理与天体物理

Journal of Biophotonics Pub Date : 2024-05-02 DOI: 10.1002/jbio.202400083

Ranjith B. Gowda, Preeta Sharan, Saara K, Mona Braim, Abdullah N. Alodhayb

{"title":"An FBG-based optical pressure sensor for the measurement of radial artery pulse pressure","authors":"Ranjith B. Gowda, Preeta Sharan,  Saara K, Mona Braim, Abdullah N. Alodhayb","doi":"10.1002/jbio.202400083","DOIUrl":"10.1002/jbio.202400083","url":null,"abstract":"One of the diagnostic tool for clinical evaluation and disease diagnosis is a pulse waveform analysis. High fidelity radial artery pulse waveforms have been investigated in clinical research to compute central aortic pressure, which has been demonstrated to be predictive of cardiovascular diseases. The radial artery must be inspected from several angles in order to obtain the best pulse waveform for estimate and diagnosis. In this study, we present the design and experimental testing of an optical sensor based on Fiber Bragg Gratings (FBG). A 3D printed device along with the FBG is used to measure the radial artery pulses. The proposed sensor is used for the purpose of quantifying the radial artery pulse waveform across major pulse position point. The suggested optical sensing system can measure the pulse signal with good accuracy. The main characteristic parameters of the pulse can then be retrieved from the processed signal for their use in clinical applications. By conducting experiments under the direction of medical experts, the pulse signals are measured. In order to experimentally validate the sensor, we used it to detect the pulse waveforms at Guan position of the wrist's radial artery in accordance with the diagnostic standards. The findings show that combining optical technologies for physiological monitoring and radial artery pulse waveform monitoring using FBG in clinical applications are highly feasible.","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140856831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0