Journal of Biophotonics最新文献

{"title":"Registration-Based Multimodal Image Calculator: A Novel Approach to the Coupling of Morphological and Gene Expression Features","authors":"Yixi Fu,&nbsp;Zibaidan Abulaiti,&nbsp;Meng Sun,&nbsp;Xu Wang,&nbsp;Xinrui Zeng,&nbsp;Zhong Zhou,&nbsp;Yaning Wang,&nbsp;Hongbo Zhang","doi":"10.1002/jbio.70300","DOIUrl":"10.1002/jbio.70300","url":null,"abstract":"<div>\u0000 \u0000 <p>Histological staining techniques offer complementary insights into tissue architecture and molecular expression. Co-registering images from the same section enables the integration of morphological and molecular features, thereby enhancing pathological diagnosis. However, achieving precise alignment remains challenging due to unreliable feature extraction or excessive degrees of freedom in current tools. To address this, we present the Registration-Based Multimodal Image Calculator (RBMIC), a software designed for accurate registration of immunofluorescence (IF) and hematoxylin and eosin (H&amp;E) images from sequentially stained tissue sections. RBMIC employs a landmark-driven framework that adaptively selects between nonlinear and linear registration algorithms to accommodate varying landmark counts and deformation complexity. By incorporating quantitative quality assessment and a hierarchical fault-tolerant mechanism, our method achieves robust, high-accuracy cross-modality registration. Applied to pathological characterization, RBMIC reveals that Down Syndrome is associated with impaired development of specific slow-twitch muscle fibers, suggesting a link to observed muscle dysfunction.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148145957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Refractive Index Spectral Fingerprints of Pathogenic Bacteria Revealed by Monte Carlo-Optimized Opto-Microfluidic Extinction Spectroscopy","authors":"Quoc-Thinh Dinh,&nbsp;Hsin-Yu Chuang,&nbsp;Dang Khoa Tong,&nbsp;Srishti Sen,&nbsp;Thi-Thu-Hien Pham,&nbsp;Thi-Hiep Nguyen,&nbsp;Cheng-Yen Kao,&nbsp;Cheng-Yang Liu","doi":"10.1002/jbio.70296","DOIUrl":"10.1002/jbio.70296","url":null,"abstract":"<p>Rapid optical differentiation of bacterial species remains challenging in turbid biological media due to strong scattering and absorption. Here, we present a physics-informed, label-free biophotonic framework for bacterial identification based on refractive index (RI) spectral signatures. An opto-microfluidic PDMS chip integrating air mirrors, microlenses, and fiber-optic probes was developed to measure transmission spectra from eight clinically relevant ESKAPEE pathogens. To address the inverse problem of optical parameter retrieval, a hybrid modeling approach combining Monte Carlo photon transport, Mie scattering theory, and a genetic algorithm was implemented to reconstruct wavelength-dependent RI spectra. The reconstructed spectra reveal clear interspecies differences, with higher RI values for <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> and lower values for <i>Staphylococcus aureus</i>. These physics-derived RI features were subsequently used for classification using a one-dimensional convolutional neural network, achieving 97.75% accuracy. This approach demonstrates the potential of RI spectral fingerprints for label-free microbial identification and on-chip biophotonic diagnostics.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.70296","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148145869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasibility of In Vivo Retinal Flavoprotein Autofluorescence Imaging in a Magnetic-Bead-Induced Rodent Glaucoma Model","authors":"Eun Ji Lee,&nbsp;Jiwon Kang,&nbsp;Jung-Ho Chung,&nbsp;Kyoung Min Lee,&nbsp;Yu Jin Roh,&nbsp;Dong Kyun Han","doi":"10.1002/jbio.70297","DOIUrl":"10.1002/jbio.70297","url":null,"abstract":"<p>Mitochondrial dysfunction and oxidative stress in retinal ganglion cells (RGCs) are critical events in the pathogenesis of glaucoma that occur prior to structural degeneration and visual field loss. Flavoprotein fluorescence (FPF) produced by oxidized mitochondrial flavoproteins offers a potential biomarker for early detection of cellular dysfunction. This study investigated the feasibility of performing FPF imaging in a rodent glaucoma model and its integration with a real-time optical coherence tomography (OCT) alignment monitor that verifies on-retina focus during fluorescence acquisition. A scanning laser ophthalmoscope-OCT system was constructed, with two photomultiplier tubes to differentiate FPF from lipofuscin autofluorescence. In the rodent glaucoma model induced by intracameral magnetic bead injection, FPF signals were detected under 450-nm excitation, while 505-nm excitation produced no signal. This proof-of-concept work demonstrates that in vivo retinal FPF imaging is feasible in this glaucoma model, which may facilitate early detection of RGC dysfunction at a cellular level.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.70297","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148059025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Accuracy of Entropy Based Image Analysis of Cervical Precancerous Cells","authors":"Jennifer Makin,&nbsp;Esther Elishaev,&nbsp;Steven M. Durbin,&nbsp;Robert A. Makin","doi":"10.1002/jbio.70294","DOIUrl":"10.1002/jbio.70294","url":null,"abstract":"<p>The objective of the study was to demonstrate the modified Bragg-Williams type analysis of digital microscopic images is an analytically valid methodology for classifying squamous cervical cytology. We analyzed 5318 single cell cervical cytology images from the SIPaKMeD and Herlev databases to calculate a Bragg-Williams order parameter (S²) using a pixel intensity histogram-based approach. A classification model for S² was created to differentiate normal, LSIL and HSIL. A set of test images from different databases were blindly classified using defined ranges to determine the sensitivity, specificity, and areas under the curve. Results showed distinct ranges of S² for Normal: 0.7181–0.8633 SD 0.1452, LSIL: 0.580–0.6626 SD 0.0826, and HSIL: 0.3289–0.550 SD 0.2211, with dysplastic cells exhibiting lower degrees of order. The S² classification model was found to have 100% sensitivity and specificity over the sample set, demonstrating the suitability of the methodology for cervical cytology classification.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.70294","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148059020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Photobiomodulation by Laser and Mechanical Systems on Low Back Pain: A Longitudinal, Randomized, Placebo-Controlled Clinical Trial","authors":"Adriana Teresa Silva Santos,&nbsp;Andreia Maria Silva Vilela Terra,&nbsp;Nivaldo Antonio Parizotto,&nbsp;Vanderlei Salvador Bagnato,&nbsp;Fernanda Rossi Paolillo","doi":"10.1002/jbio.70299","DOIUrl":"10.1002/jbio.70299","url":null,"abstract":"<div>\u0000 \u0000 <p>Approximately 80% of the world population has had, has, or will have back pain. The aim of this study is to investigate the acute and chronic effects of the synergistic application of photobiomodulation with mechanical systems on low back pain. Patients with low back pain were randomly distributed into 5 groups: 1- Vacuum therapy combined with Laser (VL); 2- Roller combined with Laser (RL); 3- Ultrasound combined with Laser (USL); 4- Punctual Laser (L); and 5- Placebo Laser (P). All treatments were performed in the posterior region of the trunk (lumbar, thoracic, and cervical), totaling 20 min, once a week for 7 sessions. Pain assessment, biomechanical measurement, kinesiological evaluation, and questionnaires to assess quality of life and well-being were performed. There were pain reduction and increased isometric strength, flexibility, and mobility, improving some aspects of life quality and well-being, especially when combined therapies were applied for rehabilitation of back pain.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148045169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On the Misinterpretation of Fluorescence-Based Quantification in Theranostic Corneal Cross-Linking","authors":"Ciro Caruso,&nbsp;Gaetano Barbaro","doi":"10.1002/jbio.70288","DOIUrl":"10.1002/jbio.70288","url":null,"abstract":"<p>We read with great interest the study by Lombardo et al. proposing a theranostic UV-A device for real-time assessment of intracorneal riboflavin concentration during corneal cross-linking (CXL) [<span>1</span>]. While the integration of diagnostic feedback into treatment delivery represents an important technological advance, several fundamental assumptions underlying this approach warrant critical reconsideration.</p><p>The central premise of the study is that fluorescence intensity can be used as a quantitative surrogate for intracorneal riboflavin concentration. However, fluorescence emission in biological tissues is not solely determined by fluorophore concentration but is strongly influenced by multiple confounding factors, including light scattering, absorption heterogeneity, and dynamic changes in tissue optical properties [<span>2, 3</span>]. In the corneal stroma, these factors evolve continuously during UV-A irradiation due to hydration changes and structural modification, directly affecting both excitation and emission pathways.</p><p>Furthermore, riboflavin is an active photosensitizer undergoing continuous photodegradation and participating in oxygen-dependent photochemical reactions. These processes introduce non-linear kinetics and concentration-dependent quenching effects, preventing a stable and direct relationship between fluorescence intensity and actual stromal concentration [<span>4, 5</span>]. Under such dynamic conditions, fluorescence should be considered a composite optical signal rather than a quantitative biomarker.</p><p>The limitations of this assumption are reflected in the reported agreement between fluorescence-based measurements and spectrophotometric analysis, which deteriorates during UV-A exposure—the very phase in which real-time monitoring is intended to provide clinically meaningful information [<span>1</span>]. The factors invoked to explain this discrepancy, such as stromal thinning and increased scattering, are intrinsic to the CXL process and therefore represent fundamental constraints rather than correctable sources of error.</p><p>More broadly, the study highlights a critical methodological issue: the attempt to control a complex photochemical process using indirect optical signals that are not uniquely related to the underlying state variables. Corneal cross-linking is governed by the interaction between UV-A irradiance, riboflavin photochemistry, and oxygen availability, with kinetics that are highly sensitive to local environmental conditions [<span>4, 6</span>]. None of these variables are directly measured by fluorescence.</p><p>The authors have nothing to report.</p><p>The authors declare no conflicts of interest.</p><p>Data sharing is not applicable to this article as no datasets were generated or analyzed during the current study.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.70288","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148045175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accelerated SPAD-Based Diffuse Optical Tomography With Data-Driven View Optimization","authors":"Linlin Li,&nbsp;Kaiqi Kuang,&nbsp;Yang Lin,&nbsp;Jianru Zhang,&nbsp;Baile Chen,&nbsp;Jingjing Jiang,&nbsp;Jiahua Jiang,&nbsp;Claudio Bruschini,&nbsp;Edoardo Charbon,&nbsp;Wuwei Ren","doi":"10.1002/jbio.70289","DOIUrl":"10.1002/jbio.70289","url":null,"abstract":"<div>\u0000 \u0000 <p>Time-domain diffuse optical tomography (TD-DOT) is a non-invasive technique that utilizes near-infrared light to visualize the optical properties in tissues, showing promising applications in tumor diagnosis and functional brain imaging. The integration of advanced SPAD arrays delivers superior data quality characterized by picosecond timing precision and increased detection channels, enabling high-fidelity reconstruction of unmixed absorption and scattering properties. These gains, however, come with longer acquisition and computation times. We introduce a data-driven view-optimization strategy that exploits the statistical richness of time-resolved measurements to reduce the number of views without sacrificing image quality. We developed a noncontact TD-DOT combining a pulsed laser, a SPAD array, and a rotational stage for view selection. Simulations and phantom experiments show that the proposed method accelerates TD-DOT while maintaining robust reconstruction, comparable to those from full-view acquisition. The accelerated TD-DOT is valuable for monitoring biological dynamics, such as cerebral hemodynamics.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148045181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-Time Intravascular Photoacoustic/Ultrasound/Optical Coherence Tomography Imaging of Pulsing Vessels Ex Vivo With and Without “Blood” Flushing","authors":"Xiaoran Wang,&nbsp;Yi Tan,&nbsp;Chengyou Shu,&nbsp;Jinke Zhang,&nbsp;Riqiang Lin,&nbsp;Yongcheng Zhang,&nbsp;Mingjian Sun,&nbsp;Zhihua Xie,&nbsp;Xiaojing Gong","doi":"10.1002/jbio.70290","DOIUrl":"10.1002/jbio.70290","url":null,"abstract":"<div>\u0000 \u0000 <p>Atherosclerotic plaque rupture causes acute cardiovascular events, necessitating accurate vulnerable plaque identification by capturing comprehensive structural and functional features, which requires novel intravascular multi-modality imaging technologies. Currently, the application of the technologies is mainly limited by moderate speed. In this study, we developed a real-time IVPA/IVUS/OCT tri-modality system by overcoming synchronization challenges and suppressing electromagnetic interference via hardware/software improvements. Ex vivo pulsing vessels imaging was performed to obtain the comprehensive information without motion artifacts. Furthermore, the alive intravascular blood environment was simulated by flowing intralipid (comparable scattering factor with blood) inside the ex vivo vessels. Benefitted from the improved imaging speed, PA and OCT signal changes of the vessels were smoothly recorded throughout manual flushing and analyzed quantitatively. To the best of our knowledge, it's the first real-time intravascular tri-modality imaging system, enabling dynamic flushing observation and providing the possibility for systematic analysis of the signal levels and distributions.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148045126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148085882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148083156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}