Journal of Biophotonics最新文献

{"title":"Raman spectroscopy with an improved support vector machine for discrimination of thyroid and parathyroid tissues","authors":"Jie Hu,&nbsp;Jinyu Xing,&nbsp;Pengfei Shao,&nbsp;Xiaopeng Ma,&nbsp;Peikun Li,&nbsp;Peng Liu,&nbsp;Ru Zhang,&nbsp;Wei Chen,&nbsp;Wang Lei,&nbsp;Ronald X. Xu","doi":"10.1002/jbio.202400084","DOIUrl":"10.1002/jbio.202400084","url":null,"abstract":"<p>The objective of this study was to discriminate thyroid and parathyroid tissues using Raman spectroscopy combined with an improved support vector machine (SVM) algorithm. In thyroid surgery, there is a risk of inadvertently removing the parathyroid glands. At present, there is a lack of research on using Raman spectroscopy to discriminate parathyroid and thyroid tissues. In this article, samples were obtained from 43 individuals with thyroid and parathyroid tissues for Raman spectroscopy analysis. This study employed partial least squares (PLS) to reduce dimensions of data, and three optimization algorithms are used to improve the classification accuracy of SVM algorithm model in spectral analysis. The results show that PLS-GA-SVM algorithm has higher diagnostic accuracy and better reliability. The sensitivity of this algorithm is 94.67% and the accuracy is 94.44%. It can be concluded that Raman spectroscopy combined with the PLS-GA-SVM diagnostic algorithm has significant potential for discriminating thyroid and parathyroid tissues.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141422207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An enhanced multimode phase imaging method based on the transport of intensity equation","authors":"Hong Cheng,&nbsp;HongYi Zhang,&nbsp;Wei Lu,&nbsp;QuanBing Zhang,&nbsp;Zijing Hu","doi":"10.1002/jbio.202400137","DOIUrl":"10.1002/jbio.202400137","url":null,"abstract":"<p>Label-free biological cell imaging relies on rapid multimode phase imaging of biological samples in natural settings. To improve image contrast, phase is encoded into intensity information using the differential interference contrast (DIC) and Zernike phase contrast (ZPC) techniques. To enable multimode contrast-enhanced observation of unstained specimens, this paper proposes an improved multimode phase imaging method based on the transport of intensity equation (TIE), which combines conventional microscopy with computational imaging. The ZPC imaging module based on adaptive aperture adjustment is applied when the quantitative phase results of biological samples have been obtained by solving the TIE. Simultaneously, a rotationally symmetric shear-based technique is used that can yield isotropic DIC. In this paper, we describe numerical simulation and optical experiments carried out to validate the accuracy and viability of this technology. The calculated Michelson contrast of the ZPC image in the resolution plate experiment increased from 0.196 to 0.394.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141422302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Label-free imaging diagnosis and collagen-optical evaluation of endometrioid adenocarcinoma with multiphoton microscopy","authors":"Bin Liu,&nbsp;Yan Liu,&nbsp;Wenju Liu,&nbsp;Tianyi Luo,&nbsp;Wei Chen,&nbsp;Cuibo Lin,&nbsp;Ling Lin,&nbsp;Shuangmu Zhuo,&nbsp;Yang Sun","doi":"10.1002/jbio.202400177","DOIUrl":"10.1002/jbio.202400177","url":null,"abstract":"<p>The assessment of tumor grade and pathological stage plays a pivotal role in determining the treatment strategy and predicting the prognosis of endometrial cancer. In this study, we employed multiphoton microscopy (MPM) to establish distinctive optical pathological signatures specific to endometrioid adenocarcinoma (EAC), while also assessing the diagnostic sensitivity, specificity, and accuracy of MPM for this particular malignancy. The MPM technique exhibits robust capability in discriminating between benign hyperplasia and various grades of cancer tissue, with statistically significant differences observed in nucleocytoplasmic ratio and second harmonic generation/two-photon excited fluorescence intensity. Moreover, by utilizing semi-automated image analysis, we identified notable disparities in six collagen signatures between benign and malignant endometrial stroma. Our study demonstrates that MPM can differentiate between benign endometrial hyperplasia and EAC without labels, while also quantitatively assessing changes in the tumor microenvironment by analyzing collagen signatures in the endometrial stromal tissue.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141422304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-dimensional morphological characterization of blood droplets during the dynamic coagulation process","authors":"Yao Li,&nbsp;Wangbiao Li,&nbsp;Xiaoman Zhang,&nbsp;Hui Lin,&nbsp;Dezi Li,&nbsp;Zhifang Li","doi":"10.1002/jbio.202400116","DOIUrl":"10.1002/jbio.202400116","url":null,"abstract":"<p>In this study, we employed a method integrating optical coherence tomography (OCT) with the U-Net and Visual Geometry Group (VGG)-Net frameworks within a convolutional neural network for quantitative characterization of the three dimensional whole blood during the dynamic coagulation process. VGG-Net architecture for the identification of blood droplets across three distinct coagulation stages including drop, gelation, and coagulation achieves an accuracy of up to 99%. In addition, the U-Net architecture demonstrated proficiency in effectively segmenting uncoagulated and coagulated portions of whole blood, as well as the background. Notably, parameters such as volume of uncoagulated and coagulated segments of the whole blood were successfully employed for the precise quantification of the coagulation process, which indicates well for the potential of future clinical diagnostics and analyses.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141422208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determining ideal offsets of spatially offset Raman spectroscopy for transcutaneous measurements—A Monte Carlo study","authors":"Keren Chen,&nbsp;Mengya sun,&nbsp;Shuo Chen","doi":"10.1002/jbio.202300564","DOIUrl":"10.1002/jbio.202300564","url":null,"abstract":"<p>Spatially offset Raman spectroscopy (SORS) is valuable for noninvasive bone assessment but requires a clearer understanding of how offset distances influence detection depth. To address this, our study devised a forward-adjoint Monte Carlo multi-layer (MCML) model to simulate photon paths in SORS, aiming to determine optimal offsets for various tissue types. We examined photon migration at offsets between 0 and 15 mm against layered phantoms of differing thicknesses and compositions to optimize the signal-to-noise ratio for bone layers. The findings highlight that optimal offsets are contingent on tissue characteristics: a metacarpal beneath 2.5 mm of tissue had an ideal offset of 6.7 mm, while a tibia with 5 mm of soft tissue required 10–11 mm. This precise calibration of SORS via MCML modeling promises substantial improvements in bone health diagnostics and potential for expansive medical applications.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141422303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic optical coherence elastography for skin burn assessment: A preliminary study on mice model","authors":"Heng Liu,&nbsp;Di Yang,&nbsp;Renfei Jia,&nbsp;Weike Wang,&nbsp;Jianwei Shang,&nbsp;Quanzhong Liu,&nbsp;Yanmei Liang","doi":"10.1002/jbio.202400028","DOIUrl":"10.1002/jbio.202400028","url":null,"abstract":"<p>Skin burns that include tissue coagulation necrosis imply variations in stiffness. Dynamic phase-sensitive optical coherence elastography (OCE) is used to evaluate the stiffness of burned skin nondestructively in this paper. The homemade dynamic OCE was initially verified through tissue-mimicking phantom experiments regarding Rayleigh wave speed. After being burned with a series of temperatures and durations, the corresponding structure and stiffness variations of mice skin were demonstrated by histological images, optical coherence tomography B-scans, and OCE elastic wave speed maps. The results clearly displayed the variation in elastic properties and stiffness of the scab edge extending in the lateral direction. Statistical analysis revealed that murine skin burned at temperatures exceeding 100°C typically exhibited greater stiffness than skin burned at temperatures below 100°C. The dynamic OCE technique shows potential application for incorporating elasticity properties as a biomechanical extension module to diagnose skin burn injuries.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141322199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnosis of dental caries based on attenuation coefficients analysis of optical coherence tomography images","authors":"Shuhao Fan,&nbsp;Huanhuan Yu,&nbsp;Zehua Guan,&nbsp;Fukang Lv,&nbsp;Zhuojun Zhou,&nbsp;Cuixia Dai","doi":"10.1002/jbio.202400031","DOIUrl":"10.1002/jbio.202400031","url":null,"abstract":"<p>Quantitative analysis of optical attenuation based on optical coherence tomography images will offer an effective method to enhance diagnostic capabilities. In this paper, the optical attenuation in demineralized caries specimens was calculated to distinguish between normal teeth and carious teeth and further to differentiate the severity of caries, and thus come to the half-automated diagnosis of dental caries. Results show that the attenuation coefficient in carious regions is approximately <span></span><math>\u0000 <mrow>\u0000 <mn>4.97</mn>\u0000 <mspace></mspace>\u0000 <msup>\u0000 <mi>mm</mi>\u0000 <mrow>\u0000 <mo>−</mo>\u0000 <mn>1</mn>\u0000 </mrow>\u0000 </msup>\u0000 <mfenced>\u0000 <mrow>\u0000 <mo>±</mo>\u0000 <mn>0.206</mn>\u0000 </mrow>\u0000 </mfenced>\u0000 </mrow></math>, while that of normal teeth is about <span></span><math>\u0000 <mrow>\u0000 <mn>3.69</mn>\u0000 <mspace></mspace>\u0000 <msup>\u0000 <mi>mm</mi>\u0000 <mrow>\u0000 <mo>−</mo>\u0000 <mn>1</mn>\u0000 </mrow>\u0000 </msup>\u0000 <mspace></mspace>\u0000 <mfenced>\u0000 <mrow>\u0000 <mo>±</mo>\u0000 <mn>0.231</mn>\u0000 </mrow>\u0000 </mfenced>\u0000 </mrow></math>. Attenuation coefficient of carious regions is 35% higher than that of normal teeth. Moreover, five classes of caries were qualified and classified based on the optical attenuation coefficient. Compared with the healthy teeth, there is a noticeable disparity in the attenuation coefficients of carious teeth, both on the surface and at the dentinoenamel junction. This study provides a method for accurate caries diagnosis, particularly in detection of early lesions and subtle structural changes.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141322198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gold/cobalt ferrite nanocomposite as a potential agent for photothermal therapy","authors":"Anna V. Motorzhina,&nbsp;Stanislav E. Pshenichnikov,&nbsp;Anton A. Anikin,&nbsp;Victor K. Belyaev,&nbsp;Alexander N. Yakunin,&nbsp;Sergey V. Zarkov,&nbsp;Valery V. Tuchin,&nbsp;Sonja Jovanović,&nbsp;Claudio Sangregorio,&nbsp;Valeria V. Rodionova,&nbsp;Larissa V. Panina,&nbsp;Kateryna V. Levada","doi":"10.1002/jbio.202300475","DOIUrl":"10.1002/jbio.202300475","url":null,"abstract":"<p>The study encompasses an investigation of optical, photothermal and biocompatibility properties of a composite consisting of golden cores surrounded by superparamagnetic CoFe₂O₄ nanoparticles. Accompanied with the experiment, the computational modeling reveals that each adjusted magnetic nanoparticle redshifts the plasmon resonance frequency in gold and nonlinearly increases the extinction cross-section at ~800 nm. The concentration dependent photothermal study demonstrates a temperature increase of 8.2 K and the photothermal conversion efficiency of 51% for the 100 μg/mL aqueous solution of the composite nanoparticles, when subjected to a laser power of 0.5 W at 815 nm. During an in vitro photothermal therapy, a portion of the composite nanoparticles, initially seeded at this concentration, remained associated with the cells after washing. These retained nanoparticles effectively heated the cell culture medium, resulting in a 22% reduction in cell viability after 15 min of the treatment. The composite features a potential in multimodal magneto-plasmonic therapies.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141312610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Model-based quantitative photoacoustic tomography with directional total variation","authors":"Jiaming Liu,&nbsp;Li Qi,&nbsp;Yanqiu Feng,&nbsp;Qiugen Hu,&nbsp;Shuangyang Zhang","doi":"10.1002/jbio.202400128","DOIUrl":"10.1002/jbio.202400128","url":null,"abstract":"<p>In photoacoustic tomography (PAT), acoustic inversion aims to recover the spatial distribution of light energy deposition within the imaging object from the signals captured by detectors. To achieve quantitative imaging, optical inversion is further employed to derive absorption coefficient (AC) images. However, limitations such as restricted detection angles and inherent noise lead to substantial artifacts and degradation in the quality of PAT images, consequently affecting the accuracy of optical inversion results. In this study, we propose a directional total variation constrained optical inversion model to reconstruct the AC image. By incorporating anatomy prior information into the optical inversion process, our method can effectively suppress artifacts in AC images while maintaining structural integrity. Simulation, phantom, and in vivo experimental results demonstrate that our method significantly improves the reconstructed AC image quality. Our method provides a reliable foundation for achieving high-quality quantitative PAT imaging.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141307695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photobiomodulation minimizes taste changes during hematopoietic cell transplantation: A randomized clinical trial","authors":"Mariana Henriques Ferreira,&nbsp;Letícia Mello Bezinelli,&nbsp;Fernanda de Paula Eduardo,&nbsp;Andrea Z. Pereira,&nbsp;Nelson Hamerschlak,&nbsp;Luciana Corrêa","doi":"10.1002/jbio.202400095","DOIUrl":"10.1002/jbio.202400095","url":null,"abstract":"<p>Prevention and treatment protocols for taste changes observed during hematopoietic cell transplantation (HCT) are not well-established. The purpose of this study was to assess the efficacy of photobiomodulation (PBM) in relieving taste changes and preventing lingual papillae atrophy. HCT patients received PBM (<i>n</i> = 42) on the tongue dorsum using an InGaAIP laser (660 nm, 100 mW, 1.1 W/cm², 8.8 J/cm²). During the HCT conditioning (T0), severe neutropenia (T1), and after neutrophil engraftment (T2), taste acuity for sweet, bitter, sour, and salty solutions, and clinical appearance of lingual papillae were compared with those of a placebo group (<i>n</i> = 43). PBM significantly reduced hypogeusia, ageusia, and parageusia at T1 and T2, and also successfully prevented papillae atrophy during all the analyzed HCT periods. In conclusion, PBM enhanced taste acuity during HCT. The decrease in papillae atrophy indicated a potential regenerative effect of this therapy on tongue mucosa.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141294084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}