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Journal of Biophotonics最新文献

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PolSpec: Polarisation-Based Detection for Versatile, Cost-Effective Rapid Hyperspectral Imaging PolSpec：基于偏振的多功能，经济高效的快速高光谱成像检测。
IF 2 3区 物理与天体物理
Journal of Biophotonics Pub Date : 2025-03-24 DOI: 10.1002/jbio.70012
Huihui Liu, Sunil Kumar, Edwin Garcia, Darren Ennis, Iain A. McNeish, Paul M. W. French
{"title":"PolSpec: Polarisation-Based Detection for Versatile, Cost-Effective Rapid Hyperspectral Imaging","authors":"Huihui Liu,&nbsp;Sunil Kumar,&nbsp;Edwin Garcia,&nbsp;Darren Ennis,&nbsp;Iain A. McNeish,&nbsp;Paul M. W. French","doi":"10.1002/jbio.70012","DOIUrl":"10.1002/jbio.70012","url":null,"abstract":"<p>“<i>PolSpec</i>” is a flexible, cost-effective approach for rapid (including single-shot) spectrally resolved imaging. While established approaches, e.g., using cascades of dichroic beamsplitters, diffractive image splitters, or mosaic filters, typically have pre-determined spectral detection bands with cost and experimental complexity scaling with the number of spectral channels, <i>PolSpec</i> uses polarisation optics to provide continuously varying transmission across a configurable spectral range to generate “spectral modulation vectors” that can represent specific spectral signatures with lower data volumes than full spectral profiles. It can be implemented with almost any detector. Here we demonstrate low-cost single-shot widefield <i>PolSpec</i>-based hyperspectral imaging using a polarisation-resolving camera.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.70012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143702487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Illuminating Immunity: A Systematic Review of Immune Cell Autofluorescence 照亮免疫：免疫细胞自发荧光系统综述》。
IF 2 3区 物理与天体物理
Journal of Biophotonics Pub Date : 2025-03-20 DOI: 10.1002/jbio.202400576
Aline Knab, Caroline Giardina, Shane T. Grey, Ewa M. Goldys, Jared M. Campbell
{"title":"Illuminating Immunity: A Systematic Review of Immune Cell Autofluorescence","authors":"Aline Knab,&nbsp;Caroline Giardina,&nbsp;Shane T. Grey,&nbsp;Ewa M. Goldys,&nbsp;Jared M. Campbell","doi":"10.1002/jbio.202400576","DOIUrl":"10.1002/jbio.202400576","url":null,"abstract":"<p>Immunophenotyping provides valuable prognostic and diagnostic information, but is technically complex and expensive. The assessment of autofluorescence is label-free and provides complex information on cell identity. However, research on its application to immunophenotyping has been heterogenous. This systematic review was carried out to identify and synthesise all available evidence on the use of autofluorescence for immunophenotyping. Eighty three full texts were included. There was a focus on neutrophils (20 papers) and macrophages (22 papers) with alveolar macrophages (13 papers) forming a subcategory. Seven studies investigated monocytes, three focused on microglia, two on dendritic cells, five on mast cells, nine on granulocytes, thirteen on eosinophils, one on erythrophagocytic cells, and one on natural killer cells. Eleven studies investigated uncategorised immune cell populations. Translation of findings into clinical immunophenotyping requires the application of reproducible methods, along with clear reporting of excitation and emission parameters, and a greater focus on clinical and primary samples.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400576","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143672122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhancing the Diagnostic Evaluation of Thyroid Functionality Using Diffuse Reflectance Spectroscopy and Regression Models 利用漫反射光谱和回归模型增强甲状腺功能的诊断评价。
IF 2 3区 物理与天体物理
Journal of Biophotonics Pub Date : 2025-03-20 DOI: 10.1002/jbio.70010
W. Anto Win Shalini, T. Rajalakshmi, S. Vasanthadev Suryakala
{"title":"Enhancing the Diagnostic Evaluation of Thyroid Functionality Using Diffuse Reflectance Spectroscopy and Regression Models","authors":"W. Anto Win Shalini,&nbsp;T. Rajalakshmi,&nbsp;S. Vasanthadev Suryakala","doi":"10.1002/jbio.70010","DOIUrl":"10.1002/jbio.70010","url":null,"abstract":"<div>\u0000 \u0000 <p>Thyroid dysfunction is a prevalent global health concern that necessitates the development of effective and non-invasive screening methods to enable early detection. The application of Diffuse Reflectance Spectroscopy (DRS) in conjunction with preprocessing and predictive models for thyroid dysfunction diagnosis is investigated. The raw spectral data captured from 31 individuals with thyroid dysfunction are subjected to spectral preprocessing techniques like, Standard Normal Variate (SNV), Multiplicative Scatter Correction (MSC), and Baseline Correction. The preprocessed data subjected to regression models like Partial Least Squares Regression (PLSR), Principal Component Regression (PCR), LASSO, Random Forest, Ridge Regression, Gaussian Process Regression (GPR), and Bayesian Regression were employed to analyse the efficacy of the models. The PLSR model in concurrence with SNV outperforms other regression models by achieving an <i>R</i>\u0000 <sup>2</sup> of 0.93, RMSE of 0.29, and MSE of 0.08, indicating low predictive error. The goodness of fit was also evaluated using Pearson's chi-squared test.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143672121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Variation of Structures and Blood Vessels of Visual Nervous System With Age 视觉神经系统结构及血管随年龄的变化。
IF 2 3区 物理与天体物理
Journal of Biophotonics Pub Date : 2025-03-18 DOI: 10.1002/jbio.70009
Ni He, Chenrui Li, Jiali Liu, Xiaolong Zhang, Liming Li, Cuixia Dai
{"title":"Variation of Structures and Blood Vessels of Visual Nervous System With Age","authors":"Ni He,&nbsp;Chenrui Li,&nbsp;Jiali Liu,&nbsp;Xiaolong Zhang,&nbsp;Liming Li,&nbsp;Cuixia Dai","doi":"10.1002/jbio.70009","DOIUrl":"10.1002/jbio.70009","url":null,"abstract":"<div>\u0000 \u0000 <p>Aging is a process of progressive functional decline associated with increasing age. The process and mechanism of aging have long been widely concerned, but long-term in vivo evaluations of the visual nervous system have not been previously reported. In this study, naturally aging mouse models were used for long-term serial evaluation, and the changes in structure and blood flow of the retina and cerebral cortex were systematically analyzed. Optical Coherence Tomography (OCT) and Optical Coherence Tomography Angiography (OCTA) were performed on mice at 3, 6, 9, and 12 months of age, respectively. Structural and vascular changes with age were quantitatively evaluated. Results show that the reduction of structural thickness and vascular density of the retinal and cerebral cortex is observed. A significant correlation is also found between structural and vascular changes in the retina and cerebral cortex, indicating a consistent impact of aging on the visual nervous system.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Advanced Intervention Effects of Pulsed and Steady Transcranial Photobiomodulation on Sleep, Mood, and EEG Signal Regulation 脉冲和稳定经颅光生物调节对睡眠、情绪和脑电图信号调节的高级干预作用。
IF 2 3区 物理与天体物理
Journal of Biophotonics Pub Date : 2025-03-18 DOI: 10.1002/jbio.70004
Xuran Zhang, Xiaojing Miao, Hui Jiang, Yi Ren, Longfei Huo, Muqing Liu, Houbo Chen
{"title":"Advanced Intervention Effects of Pulsed and Steady Transcranial Photobiomodulation on Sleep, Mood, and EEG Signal Regulation","authors":"Xuran Zhang,&nbsp;Xiaojing Miao,&nbsp;Hui Jiang,&nbsp;Yi Ren,&nbsp;Longfei Huo,&nbsp;Muqing Liu,&nbsp;Houbo Chen","doi":"10.1002/jbio.70004","DOIUrl":"10.1002/jbio.70004","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Transcranial photobiomodulation (tPBM) enhances cognitive and emotional states. We compared continuous-wave (CW) and pulsed-wave (PW) tPBM effects on 24 healthy males.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Method</h3>\u0000 \u0000 <p>Participants received 630 nm tPBM at 95 mW/cm<sup>2</sup> for 10 min: Sham, CW, or PW (500 Hz). Outcomes were assessed using the Karolinska Sleepiness Scale (KSS) (for measuring sleepiness), State-Trait Anxiety Inventory (STAI) (for assessing anxiety), Visual Analog Scale (VAS) (for measuring stress), and Beck Depression Inventory-II (BDI-II) (for evaluating depressive symptoms), and 32-channel EEG at baseline, treatment, and rest phases.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Paired <i>t</i>-tests showed PW tPBM significantly improved sleepiness, anxiety, stress, and depression scores post-intervention (<i>p</i> &lt; 0.05). ANOVA analyses indicated PW tPBM increased Alpha and Gamma band EEG power versus baseline (<i>p</i> &lt; 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>PW tPBM may improve cognitive and emotional outcomes and modulate brain activity, offering therapeutic insights.</p>\u0000 </section>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ElectroFluor Voltage-Sensitive Dyes: Comprehensive Analysis of Wavelength-Dependent Sensitivity and Cross-Channel Bleed-Through 电荧光电压敏感染料：波长依赖性灵敏度和跨通道透光的综合分析。
IF 2 3区 物理与天体物理
Journal of Biophotonics Pub Date : 2025-03-18 DOI: 10.1002/jbio.70008
Srdjan D. Antic, Ping Yan, Corey D. Acker, Olivia T. Spagnola, Zehra Y. Erol, Ozge Baser, Leslie M. Loew
{"title":"ElectroFluor Voltage-Sensitive Dyes: Comprehensive Analysis of Wavelength-Dependent Sensitivity and Cross-Channel Bleed-Through","authors":"Srdjan D. Antic,&nbsp;Ping Yan,&nbsp;Corey D. Acker,&nbsp;Olivia T. Spagnola,&nbsp;Zehra Y. Erol,&nbsp;Ozge Baser,&nbsp;Leslie M. Loew","doi":"10.1002/jbio.70008","DOIUrl":"10.1002/jbio.70008","url":null,"abstract":"<div>\u0000 \u0000 <p>New voltage-sensitive ElectroFluor (EF) dyes that emit across the visible and near-infrared spectrum (e.g., 730 nm) were recently developed. We evaluated EF-530, EF-630, and EF-730p—dyes spectrally orthogonal to green fluorescent protein (GFP)—at excitation wavelengths outside the conventional 470 nm range used for GFP-based indicators. Although previously applied in cardiac voltage imaging, their performance in neuronal tissue remains untested. We performed side-by-side comparisons using population voltage imaging in mouse cerebral cortex slices at optimal excitation wavelengths (530, 630, and 730 nm) and assessed cross-channel signal bleed-through across four excitation wavelengths (475, 530, 630, and 730 nm). All dyes produced robust optical signals at their optimal wavelengths, though non-preferred channels exhibited bleed-through with distinct amplitudes, polarities, and photobleaching patterns. These results provide detailed quantifications of EF dye performance for neuronal population imaging.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Method for Selecting the Down-Sampling Factor of Photoacoustic Image by Using Cumulative Power Difference in Frequency Domain 利用频域累积功率差选择光声图像下采样因子的方法。
IF 2 3区 物理与天体物理
Journal of Biophotonics Pub Date : 2025-03-18 DOI: 10.1002/jbio.70013
Shihao Tang, Min Wan, Yameng Zhang, Jiani Li, Ling Tao, Weitao Li
{"title":"Method for Selecting the Down-Sampling Factor of Photoacoustic Image by Using Cumulative Power Difference in Frequency Domain","authors":"Shihao Tang,&nbsp;Min Wan,&nbsp;Yameng Zhang,&nbsp;Jiani Li,&nbsp;Ling Tao,&nbsp;Weitao Li","doi":"10.1002/jbio.70013","DOIUrl":"10.1002/jbio.70013","url":null,"abstract":"<div>\u0000 \u0000 <p>As a novel non-invasive imaging technology, a constraint of photoacoustic microscopy (PAM) is its imaging speed. Often, PAM utilizes sparse spatial sampling, which necessitates extensive prior experimentation to accurately select the down-sampling factors. To overcome this limitation, this study proposes a frequency-domain evaluation index, cumulative power difference (CPD), for rapid selection of the optimal down-sampling factor. We apply the proposed CPD to photoacoustic images of the ear and brain of the mouse. The result shows that as the down-sampling factor increases, there is a similar decreasing trend in the quality of the 20 images. CPD was significantly correlated with PCC/MSE/SSIM (<i>p</i> &lt; 0.001). The findings suggest that CPD, with its ability to evaluate the quality of photoacoustic images and quickly quantify the quality loss of down-sampled images without prior inspection. This study contributes to expanding the application range of PAM and supporting its clinical prospects.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Combination of Active-Targeted Photodynamic Therapy and Photoactivated Chemotherapy for Enhanced Cancer Treatment 将主动靶向光动力疗法和光激活化疗相结合，加强癌症治疗。
IF 2 3区 物理与天体物理
Journal of Biophotonics Pub Date : 2025-03-14 DOI: 10.1002/jbio.70005
Nkune Williams Nkune, Heidi Abrahamse
{"title":"The Combination of Active-Targeted Photodynamic Therapy and Photoactivated Chemotherapy for Enhanced Cancer Treatment","authors":"Nkune Williams Nkune,&nbsp;Heidi Abrahamse","doi":"10.1002/jbio.70005","DOIUrl":"10.1002/jbio.70005","url":null,"abstract":"<p>Scientists have been actively investigating novel therapies that can effectively eradicate cancer cells with negligible side effects in normal tissues when used alone or in a combinatorial approach. Photodynamic therapy has emerged as a promising non-invasive therapy that integrates photosensitizer, oxygen, and a specific wavelength of light for the treatment of cancer. Despite encouraging outcomes yielded by PDT, conventional PSs are faced with longstanding challenges such as poor water solubility, a short half-life, and off-target toxicity. Development of nanotherapeutics has shown great potential in overcoming this issue. The tumor microenvironment is inherently hypoxic, and this promotes tumor resistance to PDT, as it is oxygen-dependent. Photoactivated chemotherapy, an oxygen-independent light-based therapy, utilizes chemotherapeutic regimens that remain inert until exposed to light, allowing target-specific activation while minimizing off-target toxicity. Integration of these techniques can improve selectivity and yield synergistic cytotoxic effects that could improve cancer treatment.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.70005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143627044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diagnosis and Post-Treatment Follow-Up Evaluation of Melasma Using Optical Coherence Tomography and Deep Learning 利用光学相干断层扫描和深度学习对黄褐斑进行诊断和治疗后随访评估。
IF 2 3区 物理与天体物理
Journal of Biophotonics Pub Date : 2025-03-14 DOI: 10.1002/jbio.70006
Xinyuan Cao, Yifeng Lu, Tingting Zhu, Zhilong Yan, Ke Li, Jianhua Mo
{"title":"Diagnosis and Post-Treatment Follow-Up Evaluation of Melasma Using Optical Coherence Tomography and Deep Learning","authors":"Xinyuan Cao,&nbsp;Yifeng Lu,&nbsp;Tingting Zhu,&nbsp;Zhilong Yan,&nbsp;Ke Li,&nbsp;Jianhua Mo","doi":"10.1002/jbio.70006","DOIUrl":"10.1002/jbio.70006","url":null,"abstract":"<div>\u0000 \u0000 <p>Melasma is a common pigmentary disorder accompanied by tissue changes in composition and structure through the epidermis and dermis. In this study, we propose to employ optical coherence tomography (OCT) combined with deep learning techniques for melasma diagnostics. Specifically, a portable spectral domain OCT system with a handheld probe was developed for clinical skin imaging. Then, a diagnostic model was built based on the VGG16 neural network by adding a spatial attention mechanism. The results show that a good differentiation with an accuracy of 94.2% can be achieved among health datasets from healthy volunteers, and melasma and tissue-around-melasma datasets from melasma patients. Moreover, the same trained model was applied to treatment evaluation, showing a good capability to assess antivascular medicine treatment. Thus, it can be concluded that OCT combined with deep learning techniques has a good potential to aid in clinical diagnosis and treatment evaluation of melasma.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optomechanical Properties of Swine Skin Tissue Treated With a Nontoxic Optical Clearing Agent 无毒光清除剂处理猪皮组织的光力学性能。
IF 2 3区 物理与天体物理
Journal of Biophotonics Pub Date : 2025-03-13 DOI: 10.1002/jbio.70007
Noemi Correa, Minji Suh, Abdul Mohizin, Jinu Sudhakaran, Kee Sung Lee, Jung Kyung Kim
{"title":"Optomechanical Properties of Swine Skin Tissue Treated With a Nontoxic Optical Clearing Agent","authors":"Noemi Correa,&nbsp;Minji Suh,&nbsp;Abdul Mohizin,&nbsp;Jinu Sudhakaran,&nbsp;Kee Sung Lee,&nbsp;Jung Kyung Kim","doi":"10.1002/jbio.70007","DOIUrl":"10.1002/jbio.70007","url":null,"abstract":"<p>This study evaluated the optomechanical and structural properties of individual macroscopic layers in swine skin tissues treated with a nontoxic optical clearing agent. The clearing agent was prepared by dissolving 2,2′-thiodiethanol in a phosphate-buffered solution and applied for up to 6 days. Prolonged clearing increased both the total and unscattered transmittance. Peaks associated with deoxygenated hemoglobin, oxygenated hemoglobin, and water showed marked reduction with extended clearing times. Histopathological examination revealed no significant structural alterations. A clearing time of approximately 4–5 days is recommended for optimal imaging, as it minimizes changes in mechanical properties. These findings support the use of optical clearing in deep tissue imaging, particularly when preserving macroscopic mechanical properties is essential, despite the inherent limitation of sample opacity.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"18 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.70007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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